CN212913171U

CN212913171U - Fluorescent camera based on indocyanine green

Info

Publication number: CN212913171U
Application number: CN202021646449.2U
Authority: CN
Inventors: 李斌
Original assignee: Second Hospital Iaxing
Current assignee: Second Hospital Iaxing
Priority date: 2020-08-10
Filing date: 2020-08-10
Publication date: 2021-04-09
Anticipated expiration: 2030-08-10

Abstract

The utility model discloses a fluorescence camera based on indocyanine green, the fluorescence camera includes: the light source emitting module can emit near-infrared light with the wavelength of 750nm-810nm, a low-coherence light source of the near-infrared light is utilized to irradiate a region to be scanned of an imaging target, and indocyanine green molecules in the imaging target are excited to generate fluorescence; the fluorescence information acquisition module is used for collecting and analyzing the fluorescence signals; an imaging module that converts the fluorescence signal into a vascular image of an imaging target organ; the display module displays the blood vessel imaging on a screen; the image output module outputs the picture of the blood vessel imaging through a USB output port or directly through a printing device; and the battery module is used for providing electric energy for each module. The fluorescence camera is mainly used for collecting, observing and evaluating the blood vessel condition in and after a target organ operation, and has the advantages of simple operation, convenient carrying, high imaging speed, good imaging effect and the like.

Description

Fluorescent camera based on indocyanine green

Technical Field

The utility model belongs to the technical field of fluorescence imaging, concretely relates to fluorescence camera based on indocyanine green.

Background

The OCT technology utilizes the basic principle of weak coherent light interference to detect back reflection or several scattering signals of different depth layers of biological tissues to incident weak coherent light, and can obtain depth signals of the biological tissues by scanning and reconstruct two-dimensional or three-dimensional structural images so as to obtain depth information of organisms. The fluorescence imaging technology uses a fluorescence probe to excite the probe by an excitation light source to generate a fluorescence signal and collects the fluorescence signal.

Various endogenous chromophores (such as deoxyhemoglobin, oxyhemoglobin, melanin, lipids) can be used as optical imaging contrast agents, which allows us to study brain function and tumor microcirculation. However, in most cases, specific biomolecules that reflect physiological changes in the disease do not produce an effective signal upon laser excitation; the use of exogenous optical probes to enhance the signal such that specific biomolecules associated with a disease but "invisible" are detected will aid in the detection and treatment of such diseases. Currently, indocyanine green (ICG) is widely used in angiography, which is a great help for diagnosis and treatment of diseases. Compared with other contrast methods (X-ray, CT, MRI, PET, etc.), ICG can be widely applied to the imaging radiography of the operation conveniently and with reduced economic cost.

Currently, imaging for ICG is typically accomplished using relatively complex fluorescence imaging equipment. A surgical visualization and medical imaging device and method using near-infrared fluorescent polymers is disclosed in the invention application No. 201880026010.9, which uses indocyanine green dye in combination with plastic and is used in enhanced surgical imaging applications, such as robot-assisted surgery. A near infrared light source, such as a 805nm laser, may be used to excite the surgical device, causing the device to emit 835nm light. Both excitation and emission wavelengths penetrate tissue and blood and provide enhanced surgical imaging. The resulting fluoroscopic image enables the user to easily determine relative tissue depth, identify tissue inhomogeneities, detect bumps or tissue irregularities, pinpoint anatomical holes, and visualize breaches.

As another example, application No. 201811641552.5 discloses a fluorescent-OCT dual-mode imaging method and an imaging device based on a green indocyanine nano-material, wherein the imaging method includes combining the green indocyanine material with a phospholipid polyethylene glycol to form a nano indocyanine material, and modifying a biological targeting molecule at the end of the polyethylene glycol molecule to obtain an ICG-PL-PEG-FA solution as a contrast agent of the fluorescent-OCT dual-mode imaging system; injecting the ICG-PL-PEG-FA solution into an imaging target, irradiating a region to be scanned of the imaging target with a low-coherence light source of near infrared light and exciting ICG-PL-PEG-FA molecules within the imaging target using a light source of 780nm wavelength to generate fluorescence; and opening an OCT imaging optical path to acquire an OCT imaging structural image with a depth range of the imaging target. The imaging device comprises a solution preparation module, a biological targeting molecule and a fluorescence-OCT dual-mode imaging system, wherein the solution preparation module is used for combining indocyanine green and phosphatided polyethylene glycol to form indocyanine green nano particles, and modifying the tail end of polyethylene glycol molecules with the biological targeting molecule to finally obtain an ICG-PL-PEG-FA solution as a contrast agent of the fluorescence-OCT dual-mode imaging system; a light source module for injecting the ICG-PL-PEG-FA solution to an imaging target, irradiating a region to be scanned of the imaging target with a low-coherence light source of near infrared light and exciting ICG-PL-PEG-FA molecules within the imaging target using a light source of 780nm wavelength to generate fluorescence; and the imaging module is used for opening an OCT imaging optical path to acquire a structural image with a depth range formed by OCT imaging of the imaging target.

In the above prior art, angiography is performed by using fluorescence reaction of indocyanine green, but the apparatus is generally complicated and inconvenient to carry and use.

Disclosure of Invention

To the defect that exists among the prior art, the utility model aims at providing a fluorescence camera based on indocyanine green, can extensively be used for the blood vessel condition collection, observation, aassessment in the target organ operation, after, have easy operation, convenient to carry, the imaging speed is fast, the imaging advantage such as effectual.

The utility model provides a technical scheme that technical problem adopted is: a fluorescent camera based on indocyanine green, the fluorescent camera essentially comprising: the device comprises a light source emitting module, a fluorescence information acquisition module, an imaging module, a display module and an image output module; wherein the content of the first and second substances,

the light source emission module can emit near-infrared light with the wavelength of 750nm-810nm, irradiate the area to be scanned of the imaging target by using a low-coherence light source of the near-infrared light, and excite indocyanine green molecules in the imaging target by using the near-infrared light source to generate fluorescence;

the fluorescence information acquisition module is used for collecting and analyzing fluorescence signals generated by indocyanine green molecules in the imaging target;

the imaging module is used for converting the fluorescence signals acquired by the fluorescence information acquisition module into blood vessel imaging of an imaging target organ;

the display module displays the blood vessel image converted by the imaging module on a screen for a user to watch in real time;

the image output module outputs the picture of the blood vessel imaging through a USB output port or directly through a printing device;

the battery module is used for providing electric energy for other modules in the fluorescent camera;

the light source emitting module, the fluorescence information acquisition module, the imaging module, the display module, the image output module and the battery module are all integrated in the fluorescence camera, and the fluorescence camera can be carried about for use.

Further, the indocyanine green is diluted according to a certain proportion, an imaging target is achieved in an intravenous injection mode, and imaging can be achieved through a fluorescence camera 3-5 minutes after injection.

Further, the indocyanine green is dissolved by using sterilized water for injection, and the solubility after dilution is 2.5 mg/ml; the injection dosage of the indocyanine green diluent is 0.1-0.5mg/Kg, the dosage is calculated according to the body weight of an examiner, and the medicine needs to be prepared for use.

Further, the wavelength of the near-infrared light emitted by the light source emission module is 780 nm.

The imaging method of the fluorescence camera comprises the following steps: firstly, injecting a diluted indocyanine green solution into a vein of a patient, and waiting for 3-5 minutes after injection; emitting near infrared light by using a light source emitting module in the fluorescence camera, irradiating a band scanning area of an imaging target and exciting indocyanine green molecules in the imaging target by using the near infrared light of 750nm-810nm to generate fluorescence; the generated fluorescence signal is collected and analyzed by a fluorescence information acquisition module; converting the blood vessel imaging of the imaging target organ through an imaging module; the display module is used for displaying the information on a screen for a user to check in real time; the image of the blood vessel is output through a USB output port or directly through a printing device, and a user carries out medical evaluation and diagnosis on a patient through the output image.

The utility model has the advantages that: compared with the prior art, the fluorescent camera based on indocyanine green can be widely used for collecting, observing and evaluating the blood vessel conditions in and after target organ operations, including blood supply conditions at two ends of a gastrointestinal anastomosis, blood vessel evaluation in and after amputated limb replantation, blood vessel evaluation of target organ tumors, sentinel lymph node imaging and in-vitro lymph node picking imaging, and has the advantages of being simple in operation, convenient to carry, high in imaging speed, good in imaging effect and the like.

Drawings

Fig. 1 is a block diagram illustrating a structure of a fluorescence camera according to the present invention.

Wherein, 1-battery module; 2-a light source emitting module; 3-a fluorescence information acquisition module; 4-an imaging module; 5-a display module; 6-image output module.

Detailed Description

The present invention will be further described with reference to the following specific embodiments. These examples are intended to illustrate the invention and are not intended to limit the scope of the invention.

As shown in fig. 1, a fluorescence camera based on indocyanine green mainly comprises: the system comprises a light source emitting module 2, a fluorescence information acquisition module 3, an imaging module 4, a display module 5 and an image output module 6; the light source emitting module 2 can emit near-infrared light with the wavelength of 780nm, irradiate the area to be scanned of the imaging target by using a low-coherence light source of the near-infrared light, and excite indocyanine green molecules in the imaging target by using the near-infrared light source to generate fluorescence; the fluorescence information acquisition module 3 is used for collecting and analyzing fluorescence signals generated by indocyanine green molecules in the imaging target; the imaging module 4 is used for converting the fluorescence signals acquired by the fluorescence information acquisition module 3 into blood vessel imaging of an imaging target organ; the display module 5 displays the blood vessel image converted by the imaging module 4 on a screen for a user to watch in real time; the image output module 6 outputs the picture of the blood vessel imaging through a USB output port or directly through a printing device; the battery module 1 is used for providing electric energy for other modules in the fluorescent camera; the light source emitting module 2, the fluorescence information collecting module 3, the imaging module 4, the display module 5, the image output module 6 and the battery module 1 are all integrated in a fluorescence camera, and the fluorescence camera can be carried about for use.

The indocyanine green is diluted according to a certain proportion, an imaging target is achieved in an intravenous injection mode, and imaging can be achieved through a fluorescence camera 3-5 minutes after injection.

Dissolving indocyanine green by using sterile water for injection, wherein the solubility of the diluted indocyanine green is 2.5 mg/ml; the injection dosage of the indocyanine green diluent is 0.1-0.5mg/Kg, the dosage is calculated according to the body weight of an examiner, and the medicine needs to be prepared for use. Need to fast before the inspection, band and drink more than 4 hours, stop intravenous administration more than 1 hour, inquire whether have iodine allergy history, prepare salvage drugs such as antiallergic medicine, choose arm vein puncture injection for use, the scalp acupuncture more than 7 is pushed with normal saline earlier, it injects right amount of dilution indocyanine green fast to prove that the vein does not have after the external leakage, within 10s, later reuse normal saline wash the pipe, observe the reaction of inspection simultaneously.

The imaging method of the fluorescence camera comprises the steps of firstly injecting diluted indocyanine green solution into the vein of a patient, and waiting for 3-5 minutes after injection; emitting near infrared light by using a light source emission module 2 in a fluorescence camera, irradiating a band scanning area of an imaging target and exciting indocyanine green molecules in the imaging target by using 780nm near infrared light to generate fluorescence; the generated fluorescence signal is collected and analyzed by a fluorescence information collecting module 3; converting the blood vessel imaging of the imaging target organ through the imaging module 4; and the data is displayed on a screen through a display module 5 for a user to check in real time; the image of the blood vessel is output through a USB output port or directly through a printing device, and a user carries out medical evaluation and diagnosis on a patient through the output image.

The utility model provides a tracing blood flow condition is realized through indocyanine green to the fluorescence camera, makes observation target blood vessel visual. For example, the evaluation of blood supply conditions at two ends of an anastomotic stoma of the gastrointestinal tract, anastomotic leakage is a common complication after gastrointestinal tract resection, and anastomotic leakage blood supply obstacle is an important reason for causing anastomotic leakage, so that the evaluation of anastomotic blood supply in the surgery is vital, and currently, the evaluation mainly depends on the experience of an operator to judge, including the colors of gastrointestinal tract walls before and after anastomosis, the bleeding conditions of the broken end of the anastomosis and whether the artery pulsation exists, and the like, and lacks objective basis, but the fluorescence camera provided by the utility model can evaluate blood vessels at two ends of the anastomotic stoma respectively before and after gastrointestinal tract anastomosis is completed, a pre-excision line is selected and marked through clinical judgment before cutting off the intestinal tract, indocyanine green is injected intravenously, the image of the fluorescence camera is used, the blood vessel condition is evaluated according to the blood vessel image, the boundary of perfusion tissues and non-perfusion tissues of the intestinal tract are marked, so as to, after the anastomotic stoma is finished, intravenous injection is carried out again, images of the fluorescence camera are used, and blood supply at two ends of the anastomotic stoma is evaluated again, so that good blood supply of the anastomotic stoma is ensured, and anastomotic leakage is reduced. The utility model provides a fluorescence camera can provide objective blood vessel image as the foundation, provides powerful help for the clinic.

The above embodiments are only used for illustrating the present invention, and not for limiting the present invention, and those skilled in the relevant technical field can make various changes and modifications without departing from the spirit and scope of the present invention, so that all equivalent technical solutions also belong to the scope of the present invention, and the protection scope of the present invention should be defined by the claims.

Claims

1. A fluorescence camera based on indocyanine green, characterized in that it essentially comprises: the device comprises a light source emitting module, a fluorescence information acquisition module, an imaging module, a display module and an image output module; wherein the content of the first and second substances,

the light source emission module can emit near-infrared light with the wavelength of 750nm-810nm, irradiate a region to be scanned of an imaging target by using a low-coherence light source of the near-infrared light, and excite indocyanine green molecules in the imaging target by using the near-infrared light source to generate fluorescence;

2. A fluorescent camera based on indocyanine green as claimed in claim 1, characterized in that: the wavelength of the near-infrared light emitted by the light source emission module is 780 nm.