CN212833791U - Micro-fluidic chip for cell function detection - Google Patents

Abstract

The utility model discloses a micro-fluidic chip that cell function detected, including upper substrate, middle level substrate and lower floor's substrate, be provided with culture medium and add the hole and culture medium outflow hole, first runner has all concatenated cultivates the chamber, cultivates the chamber and still all corresponds the intercommunication and have the second runner, and the middle part that the middle level substrate corresponds cultivates the chamber is provided with the baffle, the baffle is provided with the through-hole. The three-layer structure is overlapped, the culture cavity is provided with a partition plate to enable the culture medium and the cell sap to be layered and isolated, the cell sap below the partition plate of the culture cavity and the culture medium above the partition plate of the culture cavity have the defect of preventing the cells from flowing out along with the culture medium, and the culture cavity and the culture medium outflow hole, the cell sap adding hole and the cell sap discharge hole are all multiple, so that the drug detection on different cells is facilitated.

Description

Micro-fluidic chip for cell function detection

Technical Field

The utility model belongs to the technical field of micro-fluidic chip, especially, relate to a micro-fluidic chip that cell function detected.

Background

The microfluidic chip technology, also called microfluidic chip laboratory or lab-on-a-chip, refers to a chemical or biological laboratory constructed on a chip of several square centimeters, which integrates basic operation units related to the fields of chemistry and biology, such as sample preparation, reaction, separation, detection, cell culture, sorting, lysis, etc. onto a very small chip, and a network is formed by microchannels, so that a controllable fluid penetrates through the whole system to realize biological, chemical, medical diagnosis, and medical research and development (the initial design of the product is designed for anti-cancer drug sensitive drug toxicity experiments).

The basic characteristics and the greatest advantages of the microfluidic chip technology are as follows: the multiple unit structures can be flexibly combined on a tiny chip platform, so that the chip design is flexible and changeable and the functions are complete; the detection sample amount required by the internal structural unit of the micro chip is very small, and the large specific surface area of the micro structural unit allows the internal reagent to be rapidly diffused so as to realize rapid reaction and detection; the micro-fluidic chip technology can reduce the technical requirements on medical detection personnel, reduce the human errors of detection and further reduce the medical detection cost of patients; because the instrument is adopted for automatic completion, more accurate and sensitive detection data can be obtained.

The existing medicine has a plurality of medicines, and has a 'cocktail therapy' in cancer treatment, in particular to the test of the anticancer effect of different medicines on cancer, the stress response of different cancer cells to the same anticancer medicine is different, and the human body test is unrealistic in order to quickly detect the treatment effect of different cancer cells on the same medicine. The existing micro-fluidic chip does not correspond to the same drug, stress reaction of different cells is realized, so that the operation is very complicated, and the error of an experimental result is caused by the error of preparation of a parallel experiment due to the concentration of the drug. The existing micro-fluidic chip with multi-channel operation has the situations of cell mistaken entering a culture medium flow channel, blockage and the like in the actual operation process. Therefore, the utility model discloses it does not have a micro-fluidic chip at present to mainly solve, corresponds different cells simultaneously and carries out the problem that same medicine carries out the equivalent detection.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a micro-fluidic chip that cell function detected, it is mainly solved that there is not a micro-fluidic chip at present, corresponds different cells simultaneously and carries out the problem that same medicine carries out the equivalent and detects.

In order to solve the technical problem, the utility model discloses a realize through following technical scheme:

micro-fluidic chip for cell function detection

The plasma discharge plasma display panel comprises an upper substrate, a middle substrate and a lower substrate, wherein the upper substrate, the middle substrate and the lower substrate are sequentially overlapped and attached, and the substrates are built through plasma discharge, and the whole substrate is rectangular;

the culture medium feeding holes and the culture medium outflow holes penetrate through the upper substrate and extend to the middle substrate, first flow channels are arranged between the culture medium feeding holes and the culture medium outflow holes, and the first flow channels are arranged on one side, corresponding to the middle substrate, of the upper substrate;

the first flow passages are connected with culture cavities in series, the culture cavities are arranged in rows and correspond to the culture medium outflow holes one by one, and the culture cavities penetrate through the middle substrate and respectively extend to the upper substrate and the lower substrate;

the culture cavities are correspondingly communicated with second flow channels, the culture cavities are positioned in the middle parts corresponding to the second flow channels, cell sap adding holes and cell sap discharging holes are respectively arranged at two ends of the second flow channels, the cell sap adding holes and the cell sap discharging holes correspondingly penetrate through the upper-layer substrate and the middle-layer substrate and extend to the lower-layer substrate, and the second flow channels are arranged on one sides of the lower-layer substrate corresponding to the middle-layer substrate;

the middle part of the middle substrate corresponding to the culture cavity is provided with a partition plate, and the partition plate is provided with a through hole.

Further, the culture cavity is prism-shaped, and the cross section of the culture cavity is diamond-shaped.

Further, the second flow channel is in a herringbone shape, and the cell fluid adding holes and the cell fluid discharging holes are arranged on two sides of the herringbone in rows.

Further, the number of the culture cavities is 8.

Further, the upper substrate, the middle substrate and the lower substrate are all made of PMMA or PDMS.

The utility model discloses following beneficial effect has:

(1) different from the prior double-layer structure, the cell culture device is formed by superposing three layers, a partition plate is arranged in a culture cavity to separate and isolate a culture medium and a cell sap, the cell sap is positioned below the partition plate of the culture cavity, the culture medium is positioned above the partition plate of the culture cavity, and cells in the cell sap cannot pass through a through hole to enter the culture medium at the upper part, so that the defect that the cells flow out along with the culture medium is overcome.

(2) The separated culture medium and the cell sap are favorable for uniformity of the cell sap, when the cell sap enters the lower layer of the culture cavity, the cell sap is stood firstly so that the cell sap is solidified under the action of gel to form a stable space structure, and the cell sap is separated by adopting the partition plate, so that the culture medium is favorable for full and uniform contact with the cell sap, and the cell metabolism efficiency is improved.

(3) The culture cavity, the culture medium outlet, the cell sap feeding hole and the cell sap discharging hole are all multiple, so that drug detection on different cells is facilitated, and the culture cavity, the cell sap feeding hole and the cell sap discharging hole are mainly used for stress reaction of cancer cells with different genotypes relative to the same drug.

(4) Different from the traditional 2-dimensional cell function detection experiment, the chip can enable the cells to be in a non-adherent 3-dimensional culture state, and the cell form of the chip is closer to the state in a human body.

Of course, it is not necessary for any particular product to achieve all of the above-described advantages at the same time.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.

FIG. 1: the structure of the utility model is disassembled.

FIG. 2: the utility model discloses the structure chart bottom view.

FIG. 3: the utility model discloses cultivate chamber local structure picture enlargedly.

FIG. 4: the utility model discloses the structure chart.

FIG. 5: the utility model discloses the structure chart.

In the drawings, the components represented by the respective reference numerals are listed below:

an upper substrate 1, a middle substrate 2, a lower substrate 3, a medium inlet hole 11, a medium outlet hole 12, a first channel 13, a culture chamber 4, a second channel 31, a cell fluid inlet hole 32, a cell fluid outlet hole 33, a partition plate 41, and a through hole 42.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "open hole", "upper", "lower", "thickness", "top", "middle", "length", "inner", "peripheral", and the like, indicate positional or positional relationships, and are merely for convenience of description and simplicity of description, and do not imply that the referenced assembly or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

As shown in fig. 1-5: micro-fluidic chip for cell function detection

The plasma display panel comprises an upper substrate 1, a middle substrate 2 and a lower substrate 3, wherein the upper substrate 1, the middle substrate 2 and the lower substrate 3 are sequentially overlapped and attached, and the substrates are built through plasma discharge, and the whole substrate is rectangular;

the upper substrate 1 is provided with a culture medium feeding hole 11 and a culture medium outflow hole 12 on opposite sides of the rectangle, and the culture medium is a liquid culture medium. The culture medium feeding holes 11 and the culture medium feeding holes 12 penetrate through the upper substrate 1 and extend to the middle substrate 2, first flow channels 13 are arranged between the culture medium feeding holes 11 and the culture medium feeding holes 12, and the first flow channels 13 are arranged on one side, corresponding to the middle substrate 2, of the upper substrate 1;

the first flow passages 13 are connected with culture cavities 4 in series, the culture cavities 4 are arranged in rows and correspond to the culture medium outflow holes 12 one by one, and the culture cavities 4 penetrate through the middle substrate 2 and extend to the upper substrate 1 and the lower substrate 3 respectively; here, the culture chambers and the medium outlet holes are arranged in a row, which facilitates marking with a marker pen.

The culture chambers 4 are correspondingly communicated with second flow passages 31, the culture chambers 4 are positioned in the middle parts of the corresponding second flow passages 31, two ends of each second flow passage 31 are respectively provided with a cell sap adding hole 32 and a cell sap discharging hole 33, the cell sap adding hole 32 and the cell sap discharging hole 33 correspondingly penetrate through the upper substrate 1 and the middle substrate 2 and extend to the lower substrate 3, and the second flow passages 31 are arranged on one sides of the lower substrates 3 corresponding to the middle substrate 2;

the first flow channel and the second flow channel are circular holes, a template is etched on a polished silicon wafer through a photoetching machine, and then gel for manufacturing the substrate is poured on the silicon wafer to form the biochip with the passage. Wherein all of the medium outlet holes were designed to have an etched diameter of 2.5mm, the first flow channel diameter was 2.4mm, the cell fluid addition hole and the cell fluid outlet hole were designed to have an etched diameter of 2mm, and the second flow channel diameter was 1.12 mm.

The middle part of the middle substrate 2 corresponding to the culture cavity 4 is provided with a partition plate 41, and the partition plate 41 is provided with a through hole 42. The designed etching diameter of the middle culture cavity is 1mm, and the punching diameter of the through hole is 0.1 mm.

The utility model discloses a disposable cell culture micro-fluidic chip structure, be different from bilayer structure in the past, adopt the stack of three layer construction to form here, though first runner and second runner all communicate the culture chamber, but the position of intercommunication is different, and the culture chamber sets up the baffle and makes culture medium and cell sap layering, keep apart, be located the cell sap below the culture chamber baffle, be located the culture medium more than the culture chamber baffle, cell in the cell sap can't pass the culture medium that the through-hole got into upper portion, have the drawback that prevents the cell and accompany the culture medium and together flow.

The separated culture medium and the cell sap are beneficial to uniformity of the cell sap, the cell sap is injected firstly when the cell sap is used, a pipette is adopted to pressurize and squeeze the cell sap, gel is mixed in the cell sap, when the cell sap enters the lower layer of the culture cavity, the cell sap is firstly kept still to enable the cell sap to be solidified under the action of the gel to form a stable space structure, and the cell sap is separated by a partition plate, so that the cell sap is beneficial to preventing the first flow channel from being blocked by the cell sap, and further, the circulation of the culture medium is blocked. This also facilitates the full and even contact of the culture medium with the cell sap, improving the cell metabolism efficiency.

It should be noted that, here, culture chamber and culture medium outflow hole, cell sap add-in hole and cell sap discharge hole are all a plurality ofly, are convenient for carry out the drug detection to different cells simultaneously, and here mainly used in the stress reaction of different kinds of cancer cells relative to same kind of medicine, and the medicine dissolves in the culture medium, adopts the syringe pump to supply the culture medium constantly when using, and the culture medium that passes through cell metabolism has culture medium outflow hole to flow out, collects and detects.

As shown in fig. 3: the culture chamber 4 is prism-shaped, and the cross section of the culture chamber is diamond-shaped. The prismatic culture cavity is beneficial to the central part of the cell cavity to have no air bubbles, and the cells can be ensured to be fully contacted with the culture medium.

As shown in fig. 1: the second flow path 31 has a chevron shape, and the cell liquid addition holes 32 and the cell liquid discharge holes 33 are located in rows on both sides of the chevron shape. Because the runners and the through holes of the upper substrate, the middle substrate and the lower substrate are all modeled in a pouring mode, the built chip cavity is filled with air, and the cell sap discharge hole is arranged for air pressure balance, so that cell sap can smoothly enter the culture cavity.

As shown in fig. 1: the culture chambers 4 are provided with 8. The kit is used for simultaneously carrying out drug detection on multiple kinds of cells.

The upper substrate 1, the middle substrate 2 and the lower substrate 3 are all made of PMMA or PDMS.

It should be noted that: PDMS is biological material, and mainly used silicon board is established a model after photoetching and is pour and use gel, and itself has the gas exchange effect, does the utility model discloses mainly use the material under the majority condition, and PMMA is hard material, and is uncomfortable to build a model after photoetching with silicon board and pour, does not have the gas exchange effect, need additionally do gas exchange device, and the runner that sets up on it and perforation also all are through mechanical engraving, slightly have the difference in the precision, do not influence whole effect.

In the description herein, references to the description of "one embodiment," "an example," "a specific example," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and its practical applications, to thereby enable others skilled in the art to best understand the invention for and utilize the invention. The present invention is limited only by the claims and their full scope and equivalents.

The above embodiments are merely illustrative of the product of the present invention, and are not intended to limit the present invention in any form, and any equivalent schemes using the above disclosed technical content to make simple changes do not depart from the technical scope of the present invention.

Claims (5)

1. A microfluidic chip for cell function detection is characterized in that:

the plasma display panel comprises an upper substrate (1), a middle substrate (2) and a lower substrate (3), wherein the upper substrate (1), the middle substrate (2) and the lower substrate (3) are sequentially overlapped and attached, and the substrates are built through plasma discharge, and the whole substrate is rectangular;

the culture medium feeding device is characterized in that a plurality of culture medium feeding holes (11) and a plurality of culture medium outflow holes (12) are formed in the opposite sides of the rectangle of the upper substrate (1), the culture medium outflow holes (12) are uniformly arranged in rows, the culture medium feeding holes (11) and the culture medium outflow holes (12) penetrate through the upper substrate (1) and extend to the middle substrate (2), first flow channels (13) are arranged between the culture medium feeding holes (11) and the culture medium outflow holes (12), and the first flow channels (13) are arranged on one side, corresponding to the middle substrate (2), of the upper substrate (1);

the first flow channels (13) are connected with culture cavities (4) in series, the culture cavities (4) are arranged in rows and correspond to the culture medium outflow holes (12) one by one, and the culture cavities (4) penetrate through the middle substrate (2) and extend to the upper substrate (1) and the lower substrate (3) respectively;

the culture cavities (4) are also correspondingly communicated with second flow channels (31), the culture cavities (4) are positioned in the middle of the second flow channels (31), cell sap adding holes (32) and cell sap discharging holes (33) are respectively arranged at two ends of the second flow channels (31), the cell sap adding holes (32) and the cell sap discharging holes (33) correspondingly penetrate through the upper-layer substrate (1) and the middle-layer substrate (2) and extend to the lower-layer substrate (3), and the second flow channels (31) are arranged on one sides of the lower-layer substrate (3) corresponding to the middle-layer substrate (2);

the middle substrate (2) is provided with a partition plate (41) corresponding to the middle part of the culture cavity (4), and the partition plate (41) is provided with a through hole (42).

2. The microfluidic chip for cell function detection according to claim 1, wherein: the culture cavity (4) is prism-shaped, and the cross section of the culture cavity is diamond-shaped.

3. The microfluidic chip for cell function detection according to claim 1, wherein: the second flow channel (31) is in a herringbone shape, and the cell fluid feeding holes (32) and the cell fluid discharging holes (33) are arranged on both sides of the herringbone in rows.

4. The microfluidic chip for cell function detection according to claim 1, wherein: the number of the culture cavities (4) is 8.

5. The microfluidic chip for cell function detection according to claim 1, wherein: the upper substrate (1), the middle substrate (2) and the lower substrate (3) are all made of PMMA or PDMS.

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