CN212833710U

CN212833710U - Simple nucleic acid extraction device

Info

Publication number: CN212833710U
Application number: CN202020913681.1U
Authority: CN
Inventors: 邵建永
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-05-27
Filing date: 2020-05-27
Publication date: 2021-03-30
Anticipated expiration: 2030-05-27

Abstract

The utility model provides a simple and easy nucleic acid extraction element, including nucleic acid extraction box, nucleic acid preservation pipe joint portion and nucleic acid preservation pipe. The utility model provides a simple and easy nucleic acid extraction element novel in design is reasonable, and is small, easily carries, through utilizing annotating the liquid hole toward different cavity pre-installation treatment fluid, the flow direction of cooperation sealing bolt, water conservancy diversion baffle control treatment fluid can easily and simply accomplish nucleic acid extraction purification steps such as schizolysis, absorption, washing and elution, need not to match professional and technical personnel and extra instrument and equipment, is applicable to the outside instant nucleic acid extraction needs of laboratory, does benefit to the realization of nucleic acid instant detection, has wider application scene.

Description

Simple nucleic acid extraction device

Technical Field

The utility model relates to a biological assay equipment technical field, concretely relates to simple and easy nucleic acid extraction element.

Background

Nucleic acids are carriers of genetic information of living bodies and are essential constituent materials for all known life forms. Nucleic acids include both deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), and are mainly present in the nucleus of cells and in a state of being bound to proteins. With the rapid development of molecular biology, research and analysis of nucleic acid are being promoted and applied in clinical diagnosis, food safety, environmental detection, agriculture, forestry, animal husbandry and other fields.

Nucleic acid extraction is a very important circle in life science research, biotechnology application and gene diagnosis, and early nucleic acid extraction mainly removes impurities by phenol and chloroform extraction and combines ethanol to precipitate nucleic acid, but the method is complex to operate. At present, nucleic acid extraction reagents in the market are mainly built on a solid phase adsorption method, i.e. a purification method of adsorbing nucleic acid molecules on the surface of a certain solid phase adsorbent under specific conditions (such as pH, specific salt, etc.) and then eluting the nucleic acid molecules, and mainly comprise the following two methods: 1) centrifugal column method: the method has high extraction efficiency and good extraction effect, is widely used at present, but needs repeated centrifugation, and is not beneficial to high-flux and automatic operation; 2) magnetic bead method: the method is based on magnetic adsorption, is easy to realize automatic and high-flux operation, but has higher cost and easily influenced extraction efficiency. In addition, the two methods are complicated to operate, require certain operation skills at the same time, often need to be matched with professional technicians, need to be matched with more instruments and equipment, cannot leave a laboratory environment, are not suitable for an outdoor environment, are difficult to deal with emergency situations, and are not beneficial to the realization of the instant nucleic acid detection (POCT).

In view of the above, it is necessary to develop a nucleic acid isolation apparatus which is easy to carry and easy to handle and which can satisfy the requirement for nucleic acid isolation.

SUMMERY OF THE UTILITY MODEL

In order to solve the problem, the utility model provides a simple and easy nucleic acid extraction element, the device reasonable in design, the volume is less, easily carries, and is easy and simple to handle, need not to match professional technical personnel and extra instrument and equipment, is applicable to the outside instant nucleic acid extraction needs of laboratory, does benefit to the realization of nucleic acid instant detection (POCT).

In order to achieve the above purpose, the utility model adopts the following technical scheme: a simple nucleic acid extraction device comprises a nucleic acid extraction box, a nucleic acid preservation tube combining part and a nucleic acid preservation tube, wherein the nucleic acid extraction box comprises a first chamber, a second chamber, a third chamber, a fourth chamber and a fifth chamber; the first chamber is positioned in the center of the upper end of the nucleic acid extraction box, the top of the first chamber is provided with a first opening, the bottom of the first chamber is provided with a second opening, the first chamber is provided with a first sealing bolt, and the first sealing bolt is sleeved in the first opening and the second opening in a penetrating manner; the second chamber is positioned on the left side of the first chamber and is communicated with the first chamber through a second opening, a third opening is formed in the top of the second chamber, a fourth opening is formed in the bottom of the second chamber, a second sealing bolt is arranged in the second chamber, and the second sealing bolt penetrates through the third opening and the fourth opening to be sleeved in the second chamber; the third chamber is located below the second chamber and is communicated with the second chamber through a fourth opening, a fifth opening communicated with the binding part of the nucleic acid storage tube is arranged at the bottom of the third chamber, a first flow guide partition plate and a second flow guide partition plate are arranged on the inner wall of the third chamber, the first end of the first flow guide partition plate is arranged on the inner wall of the left side of the fourth opening, the second end of the first flow guide partition plate is arranged on the right side of the fourth opening and is arranged right above the fifth opening in a hanging manner, one end of the second flow guide partition plate is arranged on the inner wall of the left side of the fifth opening and extends leftwards, and forms a separation chamber with the inner wall of the third chamber, wherein the separation chamber is provided with an upward opening. The fourth cavity is positioned on the right side of the first cavity, a sixth opening is formed in the top of the fourth cavity, a seventh opening is formed in the bottom of the fourth cavity, a third sealing bolt is arranged in the fourth cavity, and the third sealing bolt penetrates through the sixth opening and the seventh opening and is sleeved in the sixth opening and the seventh opening; the fifth chamber is positioned below the fourth chamber and is communicated with the fourth chamber through a seventh opening, and the bottom of the fifth chamber is provided with an eighth opening which is communicated with the binding part of the nucleic acid storage tube; the nucleic acid storage tube is detachably sleeved in the binding part of the nucleic acid storage tube.

In a preferred embodiment of the present invention, the nucleic acid extraction cassette is made of one or more of polyurethane, polyethylene, polypropylene, polymethyl methacrylate, polycarbonate, cyclic olefin copolymer, polystyrene, acrylic acid, epoxy resin, rubber, and fluoroplastic.

In a preferred embodiment of the present invention, the nucleic acid extraction cassette is made of a transparent material.

In a preferred embodiment of the present invention, the nucleic acid extraction cassette has a cubic structure.

In a preferred embodiment of the present invention, the first chamber, the second chamber, and the fourth chamber further include a liquid injection hole.

In a preferred embodiment of the present invention, the first chamber is pre-filled with a sample lysis solution.

In a preferred embodiment of the present invention, the second chamber is pre-filled with a magnetic bead binding solution.

In a preferred embodiment of the present invention, the fourth chamber is pre-filled with a washing solution.

In a preferred embodiment of the present invention, the first opening, the second opening, the third opening, the fourth opening, the sixth opening and the seventh opening are all provided with a screw mosquito.

In a preferred embodiment of the present invention, the first sealing bolt, the second sealing bolt and the third sealing bolt are all screwed into the corresponding openings.

In a preferred embodiment of the present invention, a liquid outlet is disposed at the bottom of the separation chamber in the third chamber.

In a preferred embodiment of the present invention, the inner wall of the binding portion of the nucleic acid storage tube is provided with a screw thread.

In a preferred embodiment of the present invention, the outer wall of the nucleic acid storage tube is provided with a thread, and the detachable sleeve is disposed in the joint portion of the nucleic acid storage tube.

In a preferred embodiment of the present invention, the nucleic acid storage tube is a universal 1.5mL centrifuge tube or 2mL centrifuge tube with a screw cap.

The utility model has the advantages that:

1. easy to carry: the design is novel and reasonable, the volume is small, and the carrying is easy;

2. the operation is simple and convenient: the simple nucleic acid extraction device provided by the utility model pre-installs corresponding treatment liquid to different cavities through the liquid injection hole, controls the flow direction of the treatment liquid through the sealing bolt and the flow guide clapboard, and can easily and simply complete nucleic acid extraction;

3. does not need to match with professional technicians and extra instruments and equipment, is suitable for the requirement of instant nucleic acid extraction outside a laboratory, and is beneficial to the realization of instant nucleic acid detection (POCT).

Drawings

FIG. 1 is a schematic structural diagram of a simple nucleic acid extraction device according to an embodiment of the present invention;

Detailed Description

In the following, the present invention is described in further detail with reference to the drawings and the detailed description of the invention, it should be noted that, for a person skilled in the art, a plurality of improvements and decorations can be made without departing from the principle of the present invention, and these improvements and decorations are also considered as the protection scope of the present invention.

As shown in FIG. 1, the utility model provides a simple nucleic acid extracting device, which comprises a nucleic acid extracting box 1, a nucleic acid storing tube combining part 2 and a nucleic acid storing tube 3, wherein the nucleic acid extracting box 1 comprises a first chamber 11, a second chamber 12, a third chamber 13, a fourth chamber 14 and a fifth chamber 15; the first chamber 11 is located at the center of the upper end of the nucleic acid extraction cassette 1, the top of the first chamber 11 is provided with a first opening 111, the bottom of the first chamber 11 is provided with a second opening 112, the first chamber 11 is provided with a first sealing bolt 113, and the first sealing bolt 113 is sleeved in the first opening 111 and the second opening 112 in a penetrating manner; the second chamber 12 is located on the left side of the first chamber 11 and is communicated with the first chamber 11 through a second opening 112, a third opening 121 is arranged at the top of the second chamber 12, a fourth opening 122 is arranged at the bottom of the second chamber, a second sealing bolt 123 is arranged in the second chamber 12, and the second sealing bolt 123 is sleeved in the third opening 121 and the fourth opening 122 in a penetrating manner; the third chamber 13 is located below the second chamber 12 and is communicated with the second chamber 12 through a fourth opening 122, a fifth opening 131 communicated with the nucleic acid storage tube combining part 2 is arranged at the bottom of the third chamber 13, a first guide partition plate 132 and a second guide partition plate 133 are arranged on the inner wall of the third chamber 13, one end of the first guide partition plate 132 is arranged on the inner wall of the left side of the fourth opening 122, the other end of the first guide partition plate 132 is arranged on the right side of the fourth opening 122 and is suspended over the fifth opening 131, one end of the second guide partition plate 133 is arranged on the inner wall of the left side of the fifth opening 131 and extends leftwards, and a separation chamber 134 with an upward opening is formed by the third chamber 13 and the inner wall. The fourth chamber 14 is located at the right side of the first chamber 11, a sixth opening 141 is arranged at the top of the fourth chamber 14, a seventh opening 142 is arranged at the bottom of the fourth chamber 14, a third sealing bolt 143 is arranged in the fourth chamber 14, and the third sealing bolt 143 is sleeved in the sixth opening 141 and the seventh opening 142 in a penetrating manner; the fifth chamber 15 is positioned below the fourth chamber 14 and is communicated with the fourth chamber 14 through a seventh opening 142, the bottom of the fifth chamber 15 is provided with an eighth opening 151, and the eighth opening 151 is communicated with the nucleic acid storage tube combining part 2; the nucleic acid storage tube 3 is detachably sleeved in the nucleic acid storage tube combining part 2.

Preferably, the nucleic acid extraction cassette 1 is one or more of polyurethane, polyethylene, polypropylene, polymethyl methacrylate, polycarbonate, cyclic olefin copolymer, polystyrene, acrylic acid, epoxy resin, rubber, and fluoroplastic.

Preferably, the nucleic acid extraction box is made of transparent materials, so that operators can conveniently perform visual experiment operation.

Preferably, the nucleic acid extraction cassette 1 has a cubic structure.

Preferably, the first chamber 11, the second chamber 12 and the fourth chamber 14 further comprise a liquid injection hole 16.

Preferably, the first chamber 11 is pre-filled with a sample lysis solution.

Preferably, the second chamber 12 is pre-filled with a magnetic bead binding solution.

Preferably, the fourth chamber 14 is pre-filled with a washing liquid.

Preferably, the first opening 111, the second opening 112, the third opening 121, the fourth opening 122, the sixth opening 141 and the seventh opening 142 are all provided with screws.

Preferably, the first sealing bolt 113, the second sealing bolt 123 and the third sealing bolt 143 are all inserted into the corresponding openings by screw structures.

Preferably, the bottom of the separation chamber 134 in the third chamber 13 is provided with a liquid outlet 135.

Preferably, the nucleic acid preservation tube combining part 2 is provided with threads on the inner wall.

Preferably, the outer wall of the nucleic acid storage tube 3 is provided with a thread which is detachably sleeved in the nucleic acid storage tube combining part 2.

Preferably, the nucleic acid storage tube 3 is a universal 1.5mL centrifuge tube with a screw cap or a 2mL centrifuge tube.

The simple nucleic acid extraction device has the following use method:

1) pre-filling lysis solution, magnetic bead binding solution and washing solution into the first chamber 11, the second chamber 12 and the fourth chamber 14 through the injection hole 16, and simultaneously, rotatably sleeving the nucleic acid storage tube 3 on the nucleic acid storage tube binding part 2 of the nucleic acid extraction box 1;

2) adding a sample to be detected into a first chamber 11 pre-filled with a lysis solution, and carrying out sample lysis;

3) when the sample is fully lysed and the nucleic acid is released into the solution, the first sealing plug 113 is rotated to open the second opening 112, and the released nucleic acid flows into the second chamber 12 along with the lysis solution and is uniformly mixed with the magnetic bead binding solution;

4) after the magnetic beads in the magnetic bead binding solution fully adsorb the released nucleic acid, the nucleic acid extraction cassette 1 is erected in the forward direction, the second sealing plug 123 is rotated, the fourth opening 122 is opened, so that the liquid in the second cavity 12 flows into the third cavity 13, flows to the fifth opening 131 through a flow channel L1 formed by the diversion partition board of the third cavity 13 and the inner wall of the third cavity, and finally flows into the nucleic acid storage tube 3;

5) the nucleic acid storage tube 3 of the simple nucleic acid extraction device is placed in a magnetic rack, after the magnetic beads are separated from the liquid, the whole simple nucleic acid extraction device placed in the magnetic rack is inclined towards the left, and at the moment, the liquid in the nucleic acid storage tube 3 is completely transferred into the separation chamber 134 of the third chamber through the diversion partition plate of the third chamber and the flow channel L2 formed by the inner wall of the third chamber and is discharged to the outside of the box through the liquid outlet 135 at the bottom of the third chamber 13;

6) the third sealing plug 143 is screwed to open the seventh opening 142, so that the washing solution in the fourth chamber 14 flows into the fifth chamber 15, and finally flows into the nucleic acid storage tube 3 through the eighth opening 151 of the fifth chamber 15;

7) the nucleic acid storage tube 3 of the simple nucleic acid extraction device is moved out of the magnetic frame and is inclined rightwards, after the magnetic beads are shaken and washed for a plurality of times, the simple nucleic acid extraction device is erected, and the magnetic beads flow into the nucleic acid storage tube 3;

8) and (5) repeating the step 5) to obtain magnetic beads after extraction and purification of the nucleic acid-bound samples, detaching the nucleic acid preservation tube 3, adding a nucleic acid eluent to elute the nucleic acids bound on the magnetic beads, and preserving for later use.

The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.

Claims

1. A simple nucleic acid extraction device is characterized by comprising a nucleic acid extraction box, a nucleic acid storage tube combining part and a nucleic acid storage tube, wherein the nucleic acid extraction box comprises a first chamber, a second chamber, a third chamber, a fourth chamber and a fifth chamber; the first chamber is positioned in the center of the upper end of the nucleic acid extraction box, the top of the first chamber is provided with a first opening, the bottom of the first chamber is provided with a second opening, the first chamber is provided with a first sealing bolt, and the first sealing bolt is sleeved in the first opening and the second opening in a penetrating manner; the second chamber is positioned on the left side of the first chamber and is communicated with the first chamber through a second opening, a third opening is formed in the top of the second chamber, a fourth opening is formed in the bottom of the second chamber, a second sealing bolt is arranged in the second chamber, and the second sealing bolt penetrates through the third opening and the fourth opening to be sleeved in the second chamber; the third chamber is positioned below the second chamber and is communicated with the second chamber through a fourth opening, a fifth opening communicated with the binding part of the nucleic acid storage tube is arranged at the bottom of the third chamber, a first flow guide partition plate and a second flow guide partition plate are arranged on the inner wall of the third chamber, the first end of the first flow guide partition plate is arranged on the inner wall of the left side of the fourth opening, the second end of the first flow guide partition plate is arranged on the right side of the fourth opening and is arranged right above the fifth opening in a hanging manner, one end of the second flow guide partition plate is arranged on the inner wall of the left side of the fifth opening and extends leftwards, and forms a separation chamber with an upward opening with the inner wall of the third chamber; the fourth cavity is positioned on the right side of the first cavity, a sixth opening is formed in the top of the fourth cavity, a seventh opening is formed in the bottom of the fourth cavity, a third sealing bolt is arranged in the fourth cavity, and the third sealing bolt penetrates through the sixth opening and the seventh opening and is sleeved in the sixth opening and the seventh opening; the fifth chamber is positioned below the fourth chamber and is communicated with the fourth chamber through a seventh opening, and the bottom of the fifth chamber is provided with an eighth opening which is communicated with the binding part of the nucleic acid storage tube; the nucleic acid storage tube is detachably sleeved in the binding part of the nucleic acid storage tube.

2. The simple nucleic acid extracting apparatus of claim 1, wherein the nucleic acid extracting cassette is made of one or more of polyurethane, polyethylene, polypropylene, polymethyl methacrylate, polycarbonate, cyclic olefin copolymer, polystyrene, acrylic acid, epoxy resin, rubber, and fluoroplastic.

3. The simple nucleic acid isolation apparatus according to claim 1, wherein the nucleic acid isolation cassette has a cubic structure.

4. The simple nucleic acid isolation apparatus of claim 1, wherein the first chamber, the second chamber, and the fourth chamber further comprise a liquid injection hole.

5. The simple nucleic acid isolation apparatus of claim 1, wherein the first opening, the second opening, the third opening, the fourth opening, the sixth opening, and the seventh opening are provided with a mosquito coil.

6. The simple nucleic acid extracting apparatus of claim 1, wherein the first sealing plug, the second sealing plug and the third sealing plug are disposed in the corresponding openings by screws.

7. The simple nucleic acid extraction device of claim 1, wherein the bottom of the separation chamber in the third chamber is provided with a liquid outlet.

8. The simple nucleic acid isolation apparatus according to claim 1, wherein the inner wall of the binding portion of the nucleic acid storage tube is provided with a screw thread.

9. The simple nucleic acid extracting apparatus according to claim 1, wherein the outer wall of the nucleic acid storage tube is provided with a screw thread and detachably fitted in the binding portion of the nucleic acid storage tube.

10. The simple nucleic acid isolation apparatus according to claim 9, wherein the nucleic acid storage tube is a universal 1.5mL centrifuge tube with a screw cap or a 2mL centrifuge tube.