CN212755725U

CN212755725U - Mouse fecaluria separation and collection device

Info

Publication number: CN212755725U
Application number: CN202020733448.5U
Authority: CN
Inventors: 安艳; 周淼; 黄琳; 周吉全; 段佩辰
Original assignee: Suzhou University
Current assignee: Suzhou University
Priority date: 2020-05-07
Filing date: 2020-05-07
Publication date: 2021-03-23
Anticipated expiration: 2030-05-07

Abstract

A mouse fecaluria separation and collection device comprises a bracket, a fixing device and a collector; the bracket comprises a bracket base and a bracket rod, the bracket rod is vertically arranged on the bracket base, and a detachable fixing clamp is arranged on the bracket rod; the fixing device is fixed on the bracket through the fixing clamp; the collector is the tube-shape pipeline, and tube-shape pipeline one side is equipped with the decurrent branch pipe of slope, and tube-shape pipeline is inside to be provided with inverted funnel form pipeline, and the bottom edge of inverted funnel form pipeline closely laminates with the lower edge department of branch pipe interface, and inverted funnel form pipeline card is located inside the tube-shape pipeline. The utility model provides a mouse fecaluria separation and collection device with low preparation cost, convenient disassembly and cleaning and capable of reducing fecaluria loss.

Description

Mouse fecaluria separation and collection device

Technical Field

The utility model relates to an animal experiment technical field especially relates to a mouse fecaluria separation and collection device.

Background

In animal experiments, researchers often use mice as experimental animals. During the experiment, urine and feces of the mice are often required to be detected to analyze the metabolism and pathological conditions of the drugs in the mice, and particularly, the urine and feces are often used in pharmacokinetic research. Therefore, most animal laboratories require a mouse feces and urine separation and collection device.

At present, the mouse excrement and urine separation and collection device on the market is high in preparation cost and difficult to disassemble and clean, and excrement and urine samples to be detected are easy to lose in the experimental process. The simple excrement and urine separating and collecting device self-made by a laboratory often cannot achieve the effect of completely separating excrement and urine, and especially when mice excrete loose excrement, the excrement and urine mixture is collected.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to provide a mouse fecaluria separation and collection device, its low in preparation cost, dismantlement wash convenient, can reduce the fecaluria loss, realize mouse fecaluria separation and collection simultaneously.

In order to solve the technical problem, the utility model provides a technical scheme: a mouse fecaluria separation and collection device comprises a bracket, a fixing device and a collector;

the bracket comprises a bracket base and a bracket rod, the bracket rod is vertically arranged on the bracket base, and a detachable fixing clamp is arranged on the bracket rod;

the fixing device is fixed on the bracket through the fixing clamp;

the collector is the tube-shape pipeline, tube-shape pipeline one side is equipped with the decurrent branch pipe of slope, tube-shape pipeline inside is provided with the funnel form pipeline of falling, the bottom edge of funnel form pipeline of falling with divide pipe interface department of following closely to laminate down, the funnel form pipeline of falling card is located inside the tube-shape pipeline.

Preferably, the fixing means comprises a support table, a first holder and a second holder.

Preferably, the first fixer and the second fixer are semi-closed ring structures, and through holes are respectively formed in the left side and the right side of the semi-closed ring structures. The first fixer and the second fixer are designed to be semi-closed ring structures, so that the semi-closed ring structures are more favorable for fixing mice with different body types, and the devices cannot be replaced due to the different body types of the mice. The through holes are formed in the first fixing device and the second fixing device so as to clamp four limbs of a mouse, the upper limbs of the mouse are clamped in the left through hole and the right through hole of the first fixing device, and the lower limbs of the mouse are clamped in the left through hole and the right through hole of the second fixing device. Through the design of semi-closed loop configuration and the interval setting between first fixer and the second fixer, make the belly of mouse expose outside, can make things convenient for the experimenter to massage the mouse belly, help its defecation.

Preferably, a concave sliding groove is arranged in the vertical direction of the supporting platform.

Preferably, a first movable base is arranged on the peripheral surface of the first fixer, and the first movable base is clamped in the concave sliding groove; a second movable base is arranged on the outer peripheral surface of the second fixer and clamped in the concave sliding groove; the first fixer and the second fixer respectively realize the movement in the direction of the concave sliding groove through the slidable moving base. Design into the structure that can reciprocate along the direction of spill spout respectively with first fixer and second fixer, the experimenter can be according to the size of each mouse, fixes first fixer and second fixer in suitable position through sliding of moving the base respectively, and the convenience is fixed the mouse.

Preferably, the fixing clip is provided with a rotating piece. By the rotation of the rotating member, the fixing device is turned over forward or backward. When the fixing device is turned to a proper angle, if the fixing device is turned forwards, an included angle of 30 degrees is formed between the fixing device and the horizontal plane, tail vein injection can be directly performed on the mouse on the fixing device, integration of feces and urine separation and tail vein injection is realized, and the workload of experimental technicians is reduced. Optionally, the fixing device can be turned to other angles, so as to facilitate other subsequent experimental operations. The terms "forward" and "backward" are used in the present specification as general terms, and in order to avoid misunderstandings by other technicians, "forward" herein means counterclockwise from a right side view, and "backward" herein means clockwise from a right side view.

Preferably, the bottom edge of the inverted funnel-shaped pipeline is wrapped with a waterproof layer. The waterproof layer is tightly attached to the lower edge of the branch pipe connector. The waterproof layer is a rubber soft ring prepared from a waterproof coating, and the rubber soft ring can be waterproof and can also play a role in sealing. On the one hand, when urine flows to the waterproof layer, the urine cannot penetrate through the waterproof layer and penetrate into a space formed by the lower half part of the cylindrical pipeline and the inverted funnel-shaped pipeline. On the other hand, the characteristic of the soft rubber ring enables the bottom edge of the inverted funnel-shaped pipeline to be attached to the inner wall of the cylindrical pipeline more tightly, and urine can be prevented from remaining in the space through the gap.

Preferably, the pipe diameter of the cylindrical pipeline gradually narrows from the waterproof layer to the bottom. Mouse excrement and urine falls into behind the funnel form pipeline of falling, directly downwards, becomes the pipe diameter of tube-shape pipeline and narrows down gradually below the waterproof layer, can make the excrement and urine flow to tube-shape pipeline lower port that is out of shape, splashes, finally flows into in the excrement collecting container. The unformed feces are referred to as loose stools.

Preferably, the pipe diameter of the branch pipe is gradually narrowed. The design of the gradually narrowed branch pipe can accelerate the flow of urine in the branch pipe and shorten the urine collection time. Moreover, the branch pipe mouth is small, so that the subsequent urine collection is convenient.

Preferably, the diameter of the upper port of the inverted funnel-shaped pipeline is 5-10 mm. Under the normal condition, the anus of mouse and the distance of urethral orifice are no longer than 10mm, therefore the diameter of design funnel form pipeline upper port is less than 10mm, can guarantee when the upper port of the funnel form pipeline of the falling of collector moves to mouse anus department, and the upper port only covers mouse anus completely, and mouse urethral orifice then falls outside the upper port, through this design, make the excrement and urine of mouse fall into the funnel form pipeline, and the urine then falls into the tube-shape pipeline in the funnel form pipeline outside. If the diameter is designed to be larger than 10mm, it is likely that the upper port of the inverted funnel-shaped conduit covers the anus and the urethral orifice of the mouse at the same time during the experiment, and the effect of separating the excrement from the urine is not achieved finally.

Preferably, the orifice of the branch pipe is connected with a urine collecting container, and the lower orifice of the cylindrical pipeline is connected with a feces collecting container.

Further, the urine collection container is a graduated container. The container may be a measuring cylinder. The reading of urine volume can be directly carried out by replacing the ordinary container with the graduated container, and the loss of urine caused by the replacement of the container can also be prevented.

Further, the manure collection container is an EP tube.

Compared with the prior art, the utility model discloses an advantage and effect:

1. the utility model discloses a fixing device fixes the mouse, then collects the fecaluria of mouse. It is direct to connect fecaluria collection device in the breed cage of mouse among the prior art usually, and the mouse bruxism can cause fodder etc. to fall into fecaluria collection device to disturb and collect the sample, and the drippage of the water of feeding also can dilute the urine. The utility model discloses carry out water load to the mouse before the sampling point, then be fixed in fixing device with the mouse on, avoid directly collecting the interference of the sample that the fecaluria brought in breeding the cage. By adopting the fixing device, the influence of the feeding of the mouse on the sampling and the quantification of the fecaluria can be avoided, and the fecaluria loss caused by the movement of the mouse is reduced to a great extent. Moreover, through the design of the fixer and the concave sliding groove, the fixing device of the utility model is suitable for separating and collecting the excrement and urine of mice with different body types.

2. The utility model discloses a collector is the composite design of tube-shape pipeline and inverted funnel form pipeline. The utility model designs the upper port of the inverted funnel-shaped pipeline into an opening with the diameter of 5-10 mm by utilizing the distance between the anus and the urethral orifice of the mouse, so that the upper port completely covers the anus of the mouse but cannot cover the urethral orifice, and the excrement of the mouse falls into an excrement collecting container along the inverted funnel-shaped pipeline; and the urine enters the cylindrical pipeline on the outer side of the inverted funnel-shaped pipeline, finally flows out through the branch pipe and is collected by a urine collecting container. In the prior art, the excrement and the urine are not usually separated, and even if the excrement and the urine are separated, the excrement and the urine are difficult to be completely separated. Especially when the mice excrete loose stools, a mixture of manure and urine is collected. The utility model discloses can separate excrement and urine completely, then directly detect the urine, carry out research such as excrement occult blood to excrement and urine.

3. The utility model discloses fixing device passes through the rotation of swivel spare on the fixation clamp, can overturn to each angle towards the place ahead or towards the rear. The convenience is before gathering the fecaluria or after to being fixed in the mouse on the fixing device and carry out the multinomial experiment, like tail intravenous route etc. realizes a plurality of experimental procedure integration operations, has simplified the experiment flow, has shortened the experimental time, provides very big facility for the experimenter.

4. The utility model discloses most of parts of device all can directly obtain in the laboratory, can freely assemble, dismantle, wash, and it is very simple and convenient to operate. The utility model discloses the device has low, dismantles the convenient advantage of washing of preparation cost, and the experimenter separates and collects experimental animals like the fecaluria of mouse in the specially adapted laboratory. If the inverted funnel-shaped pipeline can be replaced by a funnel, the cylindrical pipeline can also be obtained by modifying the funnel. After the experiment, can wash support, fixing device, collector etc. the inverted funnel form pipeline in the collector can directly be taken out the dismantlement and wash from the tube-shape pipeline top. Only need during the next experiment put into the tube-shape pipeline with the funnel form pipeline of falling, the chucking, the equipment is accomplished promptly, can directly use.

Drawings

FIG. 1 is a schematic structural view of a device for separating and collecting mouse feces and urine according to an embodiment of the present invention;

fig. 2 is a schematic structural view of a fixing device in an embodiment of the present invention;

fig. 3 is a schematic structural diagram of a collector in an embodiment of the present invention.

Schematic symbol description:

1. a support; 2. a fixing device; 3. a collector; 4. a bracket base; 5. a support rod; 6. a fixing clip; 7. a tubular conduit; 8. pipe distribution; 9. an inverted funnel-shaped conduit; 10. a support table; 11. a first holder; 12. a second holder; 13. a through hole; 14. a concave chute; 15. a first mobile base; 16. a second mobile base; 17. a rotating member; 18. a waterproof layer; 19. a urine collection container; 20. a manure collection container.

Detailed Description

The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings of the present invention. The embodiments of the present invention are merely preferred embodiments, and the present invention is not limited thereto.

Examples

As shown in figures 1 and 3, the mouse excrement and urine separating and collecting device comprises a bracket 1, a fixing device 2 and a collector 3.

The support 1 comprises a support base 4 and a support rod 5, the support rod 5 is vertically arranged on the support base 4, and a detachable fixing clamp 6 is arranged on the support rod 5;

the fixing device 2 is fixed on the bracket 1 through a fixing clamp 6;

collector 3 is tube-shape pipeline 7, and tube-shape pipeline 7 one side is equipped with a slope decurrent branch pipe 8, and tube-shape pipeline 7 is inside to be provided with inverted funnel form pipeline 9, and the bottom edge of inverted funnel form pipeline 9 closely laminates with branch pipe 8 interface lower limb department, and inverted funnel form pipeline 9 card is located inside tube-shape pipeline 7.

As shown in fig. 2, the fixing device 2 includes a support table 10, a first holder 11, and a second holder 12.

The first holder 11 and the second holder 12 are semi-closed ring structures, and through holes 13 are respectively formed at left and right sides of the semi-closed ring structures.

A concave chute 14 is provided in the vertical direction of the support table 10.

A first movable base 15 is arranged on the outer peripheral surface of the first fixer 11, and the first movable base 15 is clamped in the concave sliding groove 14; a second movable base 16 is arranged on the outer peripheral surface of the second fixer 12, and the second movable base 16 is clamped in the concave sliding groove 14; the first holder 11 and the second holder 12 are each displaceable in the direction of the female runner 14 by means of a displaceable base.

The fixing clip 6 is provided with a rotary member 17.

The bottom edge of the inverted funnel-shaped pipeline 9 is wrapped with a waterproof layer 18. The waterproof layer 18 is a rubber soft ring.

The pipe diameter of the cylindrical pipe 7 gradually narrows from below the waterproof layer 18.

The pipe diameter of the branch pipe 8 gradually becomes narrow.

The diameter of the upper port of the inverted funnel-shaped pipe 9 is 6 mm.

The orifice of the branch pipe 8 is connected with a urine collecting container 19, and the lower orifice of the cylindrical pipe 7 is connected with a feces collecting container 20. The urine collection container 19 is a graduated cylinder and the feces collection container 20 is an EP tube.

After the water load is applied to the mouse, the face of the mouse is controlled to face forward, and the left and right forelimbs are respectively clamped into the left and right through holes 13 of the first fixator 11, and the left and right hind limbs are respectively clamped into the left and right through holes 13 of the second fixator 12. The first holder 11 and the second holder 12 are adjusted to appropriate heights, respectively, so that the mouse is fixed on the support table 10. Massage the abdomen of the mouse to promote defecation. The upper port of the inverted funnel-shaped duct 9 is aligned with the anus of the mouse to completely cover it, and the urethral orifice of the mouse is not covered by the upper port. Excrement and urine excreted by the mouse falls into the inverted funnel-shaped pipeline 9, urine falls into the cylindrical pipeline 7 on the outer side of the inverted funnel-shaped pipeline 9, and the excrement and urine is collected by the excrement collecting container 20. The urine flows down through the branch pipe 8 and is finally collected by the urine collection container 19.

After the feces and urine are collected, the rotating piece 17 on the fixing clamp 6 is rotated, the fixing device 2 is turned forwards, an included angle of 30 degrees is formed between the fixing device 2 and the horizontal plane, the rotating piece 17 is screwed again, the fixing device 2 is fixed at the included angle, and then the mice are subjected to tail vein injection of drugs, so that the feces and urine separation and tail vein injection integration are realized. After the experiment is finished, the support 1, the fixing device 2, the collector 3 and the like can be cleaned, and the inverted funnel-shaped pipeline 9 in the collector 3 can be directly taken out from the upper part of the cylindrical pipeline 7, detached and cleaned. During the next experiment, the inverted funnel-shaped pipeline 9 is only required to be placed into the cylindrical pipeline 7 and clamped, namely, the assembly is completed, and the inverted funnel-shaped pipeline can be directly used.

The support, funnel, graduated flask, EP pipe etc. that adopt in the above embodiment are laboratory commonly used laboratory supplies, but the utility model discloses but not only be limited to this, still can use the laboratory supplies similar with above-mentioned function as the replacement. The waterproof layer adopted in the embodiment is a rubber soft ring, and actually, the waterproof layer can also be made of other materials. The embodiment of the utility model provides an experimental animals uses the mouse as an example, can also be applied to the fecaluria separation and the collection of experimental animals such as rat in fact. The integration of excrement and urine separation and tail vein injection is realized in the above embodiment, and other operations required by experiments can be actually carried out by adjusting the angle position of the fixing device before and after excrement and urine separation, which is not limited to tail vein injection and is not described herein. In the above embodiments, the urine collection container and the feces collection container are used, and in practice, the separation and collection of feces and urine can be realized by sealing the branch pipe and the lower port of the cylindrical pipeline. However, in consideration of convenience in subsequent experiments, the nozzles are designed to be open and used for collecting urine and feces separately, and the present invention is not limited thereto.

The above-mentioned embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention is not limited thereto. Equivalent substitutes or changes made by the technical personnel in the technical field on the basis of the utility model are all within the protection scope of the utility model. The protection scope of the present invention is subject to the claims.

Claims

1. A mouse fecaluria separation and collection device is characterized by comprising a bracket, a fixing device and a collector;

the fixing device is fixed on the bracket through the fixing clamp;

2. The mouse excrement and urine separation and collection device according to claim 1, wherein said fixing means comprises a support table, a first holder and a second holder.

3. The mouse excrement and urine separating and collecting device according to claim 2, wherein the first and second retainers have a semi-closed ring structure, and through holes are formed at left and right sides of the semi-closed ring structure, respectively.

4. The mouse excrement and urine separating and collecting device according to claim 2, wherein said supporting platform has a concave chute formed in a vertical direction.

5. The device for separating and collecting mouse feces and urine according to claim 4, wherein a first movable base is provided on the outer peripheral surface of the first holder, and the first movable base is clamped in the concave chute; a second movable base is arranged on the outer peripheral surface of the second fixer and clamped in the concave sliding groove; the first fixer and the second fixer respectively realize the movement in the direction of the concave sliding groove through the slidable moving base.

6. The device for separating and collecting mouse feces and urine according to claim 1, wherein a rotating member is provided on the fixing clip.

7. The mouse manure separation and collection device of claim 1 wherein the bottom edge of the inverted funnel shaped conduit is wrapped with a waterproof layer.

8. The device for separating and collecting mouse excrement and urine according to claim 1, wherein the tube-shaped pipe has a diameter gradually narrowed from a portion below the waterproof layer.

9. The device for separating and collecting mouse excrement and urine according to claim 1, wherein the diameter of said branch pipe is gradually narrowed.

10. The device for separating and collecting mouse excrement and urine according to claim 1, wherein the diameter of the upper end of said inverted funnel-shaped pipe is 5-10 mm.

11. The device for separating and collecting mouse excrement and urine according to claim 1, wherein a urine collection container is connected to a nozzle of said branched pipe, and a excrement collection container is connected to a lower nozzle of said cylindrical pipe.