CN212741350U - Seeding tank system and mycelial ball crushing tank for culturing filamentous microorganisms - Google Patents
Seeding tank system and mycelial ball crushing tank for culturing filamentous microorganisms Download PDFInfo
- Publication number
- CN212741350U CN212741350U CN202020399446.7U CN202020399446U CN212741350U CN 212741350 U CN212741350 U CN 212741350U CN 202020399446 U CN202020399446 U CN 202020399446U CN 212741350 U CN212741350 U CN 212741350U
- Authority
- CN
- China
- Prior art keywords
- tank body
- liquid
- closed tank
- tank
- closed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The application discloses a seeding tank system and a mycelial ball crushing tank for culturing filamentous microorganisms. The system comprises: the seeding tank can contain filamentous microorganism culture solution and can culture filamentous microorganisms in the filamentous microorganism culture solution; the mycelium pellet crushing tank comprises a closed tank body, wherein the closed tank body is communicated with the interior of the seed tank through a liquid pipeline, and a mycelium pellet crushing device is arranged in the closed tank body. This application separates the filamentous microorganism culture solution of part in the seed jar to the independent breakage of mycelium pellet is carried out to the airtight jar outside the seed jar internally, be convenient for through the structural design of the airtight jar of adjustment body, improve crushing effect, through the rational design to the fluid pipeline, the limit circulates the limit and breaks, can gradually accomplish the breakage to mycelium pellet in the culture solution, in the time of convenient to use, can discharge the culture solution again, reasonable control is carried out to backward flow etc. to improve the inoculation quality of filamentous microorganism culture solution.
Description
Technical Field
The utility model relates to a microbial cultivation equipment technical field, a seeding tank system is used in filamentous microbial cultivation specifically says so.
Background
In the process of liquid culture, filamentous microorganisms are easy to form mycelium pellets, which are relatively compact mycelium forms. According to literature reports, when the mycelium pellet is proper in size, product synthesis can be promoted, and yield can be improved. However, when the diameter of the mycelium pellet is too large, the production of the product is affected, and the yield is lowered. This is because the larger diameter of the mycelium pellet hinders the transport of oxygen, nutrients and products, which ultimately affects product formation and reduces yield. In the process of strain propagation, the diameter of the mycelium pellet is increased along with the increase of the propagation stage number, and when the mycelium pellet is inoculated into a fermentation tank, the diameter of the mycelium pellet is enough to influence the product synthesis.
SUMMERY OF THE UTILITY MODEL
To the defect that exists among the prior art, the utility model aims to provide a filamentous microorganism is dona tub system for cultivation, this system can expand the mycelium pellet of cultivateing the in-process formation with the bacterial and smash into tiny hypha piece, and the hypha piece can germinate into a strain of filamentous microbial mycelium, improves the inoculation amount of next-level seed to finally control the mycelium pellet diameter in the dona tub at the synthetic length of suitable result.
In order to achieve the above purpose, the utility model adopts the technical proposal that:
the utility model provides a dona tub system is used in filamentous microorganism cultivation, include:
a seeding tank which can contain filamentous microorganism culture solution and can culture filamentous microorganisms in the filamentous microorganism culture solution,
the mycelium pellet crushing tank comprises a closed tank body, wherein the closed tank body is communicated with the interior of the seed tank through a liquid pipeline, and a mycelium pellet crushing device is arranged in the closed tank body.
In a preferred embodiment, the mycelium pellet disruption apparatus comprises a cutting blade comprising a blade portion capable of radial rotation within a specific horizontal plane in the lower and/or middle portion of the closed tank body.
In a preferred embodiment, the blade portion is bent at 160 ° along 100 °, so as to improve the cutting efficiency of the mycelium pellet.
In a preferred embodiment, the mycelium pellet breaking means comprises a rotary shaft vertically extending into the closed tank body, and one end of the cutting blade is fixed to an outer wall of the rotary shaft and rotates with the axial rotation of the rotary shaft.
In a preferred embodiment, the rotating shaft is driven by a first motor located above the closed tank;
and/or, the mycelium pellet crushing device comprises a base, the base is fixed on the inner bottom of the closed tank body, and the lower tail end of the rotating shaft is fixed on the base.
In a preferred embodiment, the liquid pipeline comprises a first liquid pipeline, the seed tank is provided with a first liquid discharge port, the closed tank body is provided with a first liquid inlet, and the first liquid discharge port is communicated with the first liquid inlet through the first liquid pipeline;
the first liquid discharge port is arranged at the bottom of the seed tank so as to discharge liquid conveniently;
the first liquid inlet is arranged in the middle or at the upper part of the closed tank body;
and a first liquid valve and/or a first circulating pump are arranged on the first liquid pipeline.
In a preferred embodiment, the liquid pipeline further comprises a second liquid pipeline, the seed tank is provided with a second liquid inlet, the closed tank body is provided with a second liquid outlet, and the second liquid inlet and the second liquid outlet are communicated through the second liquid pipeline;
the second liquid inlet is arranged at the upper part of the seed tank;
the second liquid outlet is arranged in the middle or at the upper part of the closed tank body;
and a second liquid valve and/or a second circulating pump are arranged on the second liquid pipeline.
In a preferred embodiment, the seed tank comprises a stirring device, the stirring device comprises a stirring paddle vertically extending into the seed tank and a second motor positioned above the outside of the seed tank, the stirring paddle can rotate axially under the driving of the second motor, and a paddle is arranged in the middle and/or the lower part of the stirring paddle; and defoaming slurry is arranged at the upper part of the stirring paddle.
In a preferred embodiment, the jacket type heat exchanger is arranged on the outer wall of the tank body of the seeding tank. So as to adjust the culture temperature of the filamentous microorganism culture solution in the seeding tank.
In a preferred embodiment, a first discharge hole is formed in the upper part of the seed tank, and a first discharge hole is formed in the bottom of the seed tank;
and/or a second discharge hole is arranged at the upper part of the closed tank body, and a second discharge hole is arranged at the bottom of the closed tank body.
The utility model also provides a mycelium pellet crushing jar, include: the device comprises a closed tank body, wherein a mycelium pellet crushing device is arranged in the closed tank body and comprises a cutting blade, the cutting blade comprises a blade part, and the blade part can rotate radially in a specific horizontal plane at the lower part and/or the middle part in the closed tank body; the blade part is bent at 100 DEG and 160 deg.
In the above mycelium pellet crushing tank, the mycelium pellet crushing device comprises a rotating shaft vertically extending into the closed tank body, and one end of the cutting blade is fixed on the outer wall of the rotating shaft and rotates along with the axial rotation of the rotating shaft;
the rotating shaft is driven by a first motor positioned above the closed tank body;
the mycelium pellet crushing device comprises a base, the base is fixed on the inner bottom of the closed tank body, and the lower tail end of the rotating shaft is fixed on the base;
and/or a first liquid inlet is arranged at the middle part or the upper part of the closed tank body, and a second liquid outlet is arranged at the middle part or the upper part of the closed tank body;
and/or a second discharge hole is arranged at the upper part of the closed tank body, and a second discharge hole is arranged at the bottom of the closed tank body.
The advantages of the utility model:
because the seed jar is liquid too much, the mycelium pellet is dispersed relatively, it is not good to directly carry out crushing effect to the mycelium pellet in the seed jar, in addition, the too high temperature in the seed jar, pH and other cultivation condition reasons make broken part impaired easily, reduce life. Separate the independent breakage of mycelium pellet to the airtight jar outside the seed jar with the filamentous microorganism culture solution of part in the seed jar is internal, be convenient for through the structural design of the airtight jar of adjustment body, improve crushing effect, through reasonable pipeline design, the limit is backward flow broken simultaneously, can progressively accomplish the breakage to mycelium pellet in the culture solution, in the time of convenient to use, can discharge culture solution again, return flow etc. carries out reasonable control to improve the inoculation quality of filamentous microorganism culture solution.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the application and together with the description serve to explain the application and not to limit the application. In the drawings:
FIG. 1 is a schematic view showing the construction of a seed tank system for culturing filamentous microorganisms.
Fig. 2 is a plan view of the cutting blade mounted on the rotating shaft.
The labels in the figure are as follows:
1 dona tub, 2 filiform microorganism culture solution, 3 airtight jar bodies, 4 liquid pipeline, 6 cutting blade, 7 cutting parts, 8 rotation axes, 9 first motor, 10 bases, 11 first liquid pipeline, 12 first drain outlets, 13 first inlet, 14 first liquid valve, 15 first circulating pump, 16 second liquid pipeline, 17 second inlet, 18 second drain outlets, 19 second liquid valve, 20 stirring rake, 21 second motor, 22 paddle, 23 defoaming thick liquid, 24 jacketed heat exchanger, 25 first drain outlets, 26 first discharge outlets, 27 second drain outlets, 28 second discharge outlets.
Detailed Description
In order to more clearly explain the overall concept of the present application, the following detailed description is given by way of example in conjunction with the accompanying drawings.
As shown in FIG. 1, the utility model provides a dona tub system for filamentous microorganism culture includes:
a seeding tank 1, which can contain filamentous microorganism culture solution 2 inside and can culture filamentous microorganisms in the filamentous microorganism culture solution 2,
the mycelium pellet crushing tank comprises a closed tank body 3, wherein the closed tank body 3 is communicated with the interior of a seed tank 1 through a liquid pipeline 4, and a mycelium pellet crushing device is arranged in the closed tank body 3.
In a preferred embodiment, the mycelium pellet crushing device comprises a cutting blade 6, wherein the cutting blade 6 comprises a blade part 7, and the blade part 7 can rotate radially in a specific horizontal plane at the lower part and/or the middle part in the closed tank body 3.
In a preferred embodiment, the blade portion 7 is bent at an angle of 100-.
In a preferred embodiment, the mycelium pellet crushing device comprises a rotating shaft 8, the rotating shaft 8 vertically extends into the closed tank 3, and one end of the cutting blade 6 is fixed on the outer wall of the rotating shaft 8 and rotates along with the axial rotation of the rotating shaft.
In a preferred embodiment, a first motor 9 is arranged above the closed tank 3, and the rotating shaft 8 is driven by the first motor 9;
and/or the mycelium pellet crushing device further comprises a base 10, the base 10 is fixed on the inner bottom of the closed tank body 3, and the lower tail end of the rotating shaft 8 is fixed on the base 10.
In a preferred embodiment, the liquid pipeline 4 comprises a first liquid pipeline 11, the seeding tank 1 is provided with a first liquid discharge port 12, the closed tank body 3 is provided with a first liquid inlet 13, and the first liquid discharge port 12 is communicated with the first liquid inlet 13 through the first liquid pipeline 11;
the first liquid discharge port 12 is arranged at the bottom of the seed tank 1; so as to facilitate the discharge of the liquid into the closed tank 3;
the first liquid inlet 13 is arranged in the middle or at the upper part of the closed tank body 3;
the first liquid pipeline 11 is provided with a first liquid valve 14 and/or a first circulating pump 15.
In a preferred embodiment, the liquid pipeline 4 further comprises a second liquid pipeline 16, the seeding tank 1 is provided with a second liquid inlet 17, the closed tank body 3 is provided with a second liquid outlet 18, and the second liquid inlet 17 and the second liquid outlet 18 are communicated through the second liquid pipeline 16;
the second liquid inlet 17 is arranged at the upper part of the seed tank 1;
the second liquid outlet 18 is arranged at the middle part or the upper part of the closed tank body 3;
the second liquid line 16 is provided with a second liquid valve 19 and/or a second circulation pump (not shown).
The seeding tank 1 comprises a stirring device, the stirring device comprises a stirring paddle 20 vertically extending into the seeding tank 1 and a second motor 21 positioned above the outside of the seeding tank 1, the stirring paddle 20 is driven by the second motor 21 to axially rotate, and blades 22 are arranged in the middle and/or the lower part of the stirring paddle 20; the upper part of the stirring paddle 20 is provided with defoaming paddle 23; a jacketed heat exchanger 24 is arranged on the outer wall of the seeding tank 1;
in a preferred embodiment, a first discharge port 25 is arranged at the upper part of the seeding tank 1, and a first discharge port 26 is arranged at the bottom of the seeding tank 1;
and/or a second discharge port 27 is arranged at the upper part of the closed tank body 3, and a second discharge port 28 is arranged at the bottom of the closed tank body 3, so that part of liquid can be taken out conveniently, and the broken condition of the mycelium pellets can be observed.
As shown in fig. 2, the number of the cutting blades 6 is 3, and the cutting blades are uniformly arranged at one height of the rotating shaft 8 in a wheel shape, and may also be different heights, and the number of the cutting blades 6 may also be 2, 4 or 5, which is determined according to actual needs.
The following examples illustrate the method of use of the present invention:
after the filamentous microorganism culture solution 2 in the seeding tank 1 is cultured for a period of time, the first liquid valve 14 and the first circulating pump 15 are opened, the filamentous microorganism culture solution 2 at the bottom of the seeding tank 1 flows through the closed tank body 3, meanwhile, the mycelium pellet crushing device is started, the mycelium pellets are crushed by the cutting blade 6 in the rotating process, then the second liquid valve 19 is opened, the crushed mycelium pellets and the culture solution thereof flow back to the seeding tank 1 from the upper part of the seeding tank 1, the mycelium pellets in the culture solution 2 are crushed while circulating, and finally, the reproductive capacity or the inoculation quality of the filamentous microorganism culture solution 2 in the seeding tank 1 is improved.
Those not described in detail in this specification are within the skill of the art. The above description is only an example of the present application and is not intended to limit the present application. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.
Claims (10)
1. A seeding tank system for filamentous microorganism culture, the system comprising:
a seeding tank which can contain filamentous microorganism culture solution and can culture filamentous microorganisms in the filamentous microorganism culture solution,
the mycelium pellet crushing tank comprises a closed tank body, wherein the closed tank body is communicated with the interior of the seed tank through a liquid pipeline, and a mycelium pellet crushing device is arranged in the closed tank body.
2. A seeding tank system for culturing filamentous microorganisms according to claim 1, wherein the mycelium pellet crushing means comprises a cutting blade comprising an edge part capable of radial rotation in a specific horizontal plane at the lower part and/or the middle part of the closed tank body.
3. The seeding tank system for filamentous microorganism culture as claimed in claim 2, wherein the blade is bent at 100-160 °.
4. A seeding tank system for culturing filamentous microorganisms according to claim 2, wherein the mycelium pellet crushing means comprises a rotating shaft vertically extending into the closed tank body, and one end of the cutting blade is fixed to an outer wall of the rotating shaft and rotates with the axial rotation of the rotating shaft.
5. The seeding tank system for culturing filamentous microorganisms according to claim 4, wherein a first motor is arranged above the closed tank body, and the rotating shaft is driven by the first motor;
and/or, the mycelium pellet crushing device comprises a base, the base is fixed on the inner bottom of the closed tank body, and the lower tail end of the rotating shaft is fixed on the base.
6. The seeding tank system for culturing the filamentous microorganisms according to claim 1 or 2, wherein the liquid pipeline comprises a first liquid pipeline, the seeding tank is provided with a first liquid discharge port, the closed tank body is provided with a first liquid inlet, and the first liquid discharge port is communicated with the first liquid inlet through the first liquid pipeline;
the first liquid discharge port is arranged at the bottom of the seed tank;
the first liquid inlet is arranged in the middle or at the upper part of the closed tank body;
and a first liquid valve and/or a first circulating pump are arranged on the first liquid pipeline.
7. The seeding tank system for culturing the filamentous microorganisms as claimed in claim 6, wherein the liquid pipeline further comprises a second liquid pipeline, the seeding tank is provided with a second liquid inlet, the closed tank body is provided with a second liquid outlet, and the second liquid inlet and the second liquid outlet are communicated through the second liquid pipeline;
the second liquid inlet is arranged at the upper part of the seed tank;
the second liquid outlet is arranged in the middle or at the upper part of the closed tank body;
and a second liquid valve and/or a second circulating pump are arranged on the second liquid pipeline.
8. The seeding tank system for culturing filamentous microorganisms according to claim 1 or 2, wherein the seeding tank comprises a stirring device, the stirring device comprises a stirring paddle vertically extending into the seeding tank and a second motor located above and outside the seeding tank, the stirring paddle can rotate axially under the drive of the second motor, a paddle is arranged in the middle and/or lower part of the stirring paddle, and defoaming paddle is arranged at the upper part of the stirring paddle;
and/or a jacketed heat exchanger is arranged on the outer wall of the tank body of the seed tank;
and/or a first discharge port is arranged at the upper part of the seed tank, and a first discharge port is arranged at the bottom of the seed tank;
and/or a second discharge hole is arranged at the upper part of the closed tank body, and a second discharge hole is arranged at the bottom of the closed tank body.
9. The utility model provides a mycelial ball crushing jar which characterized in that: the method comprises the following steps: the device comprises a closed tank body, wherein a mycelium pellet crushing device is arranged in the closed tank body and comprises a cutting blade, the cutting blade comprises a blade part, and the blade part can rotate radially in a specific horizontal plane at the lower part and/or the middle part in the closed tank body; the blade part is bent at 100 DEG and 160 deg.
10. The mycelial sphere disruption tank of claim 9, wherein: the mycelium pellet crushing device comprises a rotating shaft, the rotating shaft vertically extends into the closed tank body, and one end of the cutting blade is fixed on the outer wall of the rotating shaft and rotates along with the axial rotation of the rotating shaft;
the rotating shaft is driven by a first motor positioned above the closed tank body;
the mycelium pellet crushing device comprises a base, the base is fixed on the inner bottom of the closed tank body, and the lower tail end of the rotating shaft is fixed on the base;
and/or a first liquid inlet is arranged at the middle part or the upper part of the closed tank body, and a second liquid outlet is arranged at the middle part or the upper part of the closed tank body;
and/or a second discharge hole is arranged at the upper part of the closed tank body, and a second discharge hole is arranged at the bottom of the closed tank body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020399446.7U CN212741350U (en) | 2020-03-25 | 2020-03-25 | Seeding tank system and mycelial ball crushing tank for culturing filamentous microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020399446.7U CN212741350U (en) | 2020-03-25 | 2020-03-25 | Seeding tank system and mycelial ball crushing tank for culturing filamentous microorganisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212741350U true CN212741350U (en) | 2021-03-19 |
Family
ID=74985606
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020399446.7U Active CN212741350U (en) | 2020-03-25 | 2020-03-25 | Seeding tank system and mycelial ball crushing tank for culturing filamentous microorganisms |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212741350U (en) |
-
2020
- 2020-03-25 CN CN202020399446.7U patent/CN212741350U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101671625B (en) | Method and device for preparing trichoderma conidium by liquid state submerged fermentation | |
| CN112251338B (en) | Enzyme preparation microbial strain liquid fermentation device and fermentation process thereof | |
| CN108795704B (en) | Oxygen supply fermentation system | |
| CN207828283U (en) | A kind of biological fermentation tank with highly effective oxygen dissolving effect | |
| CN102321537A (en) | Bubbling bioreactor used for plant tissue culture | |
| CN212741350U (en) | Seeding tank system and mycelial ball crushing tank for culturing filamentous microorganisms | |
| CN108220139B (en) | Differential double-screw stirring integrated solid fermentation device | |
| CN209428528U (en) | A kind of edible fungus fermented tank | |
| CN117229881B (en) | Bioreactor for filamentous fungi and application thereof | |
| CN207062292U (en) | A kind of filamentous fungi fermentation fermentation tank | |
| CN209759442U (en) | Liquid fermentation tank with double stirrers | |
| CN115558579B (en) | Fermentation equipment | |
| CN107382401A (en) | A kind of organic fertilizer high temperature rapid fermenting device | |
| CN113812302B (en) | Method for preparing liquid edible fungus strain | |
| CN206680461U (en) | A kind of penicillin production installation for fermenting | |
| CN210237605U (en) | Energy-saving fermentation tank | |
| CN208604121U (en) | A kind of spawn fermentation tank | |
| CN208200973U (en) | A kind of production equipment of serialization supply logarithmic growth phase bacterium solution | |
| CN107603870B (en) | Digestion and filtration device for microcarrier cell culture | |
| CN218969125U (en) | Microbial fertilizer preparation fermentation tank | |
| CN220685097U (en) | Multifunctional strain fermentation tank | |
| CN205275614U (en) | Cell is agitating unit for jar | |
| CN218404162U (en) | Strain fermentation tank | |
| CN211832184U (en) | Edible mushroom liquid strain air-lift fermentation device | |
| CN220183274U (en) | Energy-saving efficient magnetic stirring driving type probiotics fermentation culture device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |