CN212532998U - Microorganism sampling device - Google Patents

Abstract

The utility model discloses a microorganism sampling device, include: a sterile culture vessel having a culture medium in an interior chamber; the pumping and injecting device is used for pumping a water sample and injecting the water sample in the pumping and injecting device into the sterile culture container; wherein, two ends of the three-way sterile tube are respectively communicated with the sterile culture container and the pumping and injecting device, and the other end of the three-way sterile tube is used for sampling; and the control device is used for controlling the on-off of each flow path of the three-way sterile pipe. The utility model provides a microorganism sampling device can be at the aseptic culture medium of adding of water sample that the sampling scene was gathered, and it is high to detect the accuracy.

Description

Microorganism sampling device

Technical Field

The utility model relates to a water treatment field especially relates to microorganism sampling device.

Background

The collection of water samples is an important component of water quality microbiological testing, and is used for testing the number and the condition of samples. In order to avoid pollution, the collected water sample containing microorganisms needs to be added into a culture medium for culture in an aseptic environment, and the aseptic environment does not exist in a sampling site.

The water sample is collected from a sampling site and then can be transported back to a microorganism sterile laboratory within a certain transportation time, the microorganism detection is an experiment with very strong timeliness, and the sample can not be guaranteed to arrive at the laboratory within a specified time due to factors such as sampling place, distance and the like, so that the accuracy of a detection result is influenced.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a microorganism sampling device can be at the aseptic culture medium of adding of water sample of sampling scene with gathering, and it is high to detect the accuracy.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model discloses a microorganism sampling device, include:

a sterile culture vessel having a culture medium in an interior chamber;

the pumping and injecting device is used for pumping a water sample and injecting the water sample in the pumping and injecting device into the sterile culture container;

wherein, two ends of the three-way sterile tube are respectively communicated with the sterile culture container and the pumping and injecting device, and the other end of the three-way sterile tube is used for sampling;

and the control device is used for controlling the on-off of each flow path of the three-way sterile pipe.

The utility model has the advantages that: during sampling, the control device is operated, firstly, a flow path between the three-way sterile pipe and the sterile culture container is blocked, the sampling end of the three-way sterile pipe is communicated with the flow path of the pumping device, a water sample is pumped into the pumping device through the pumping device, after the required amount is reached, the control device is operated again, the flow path between the sterile culture container on the three-way sterile pipe and the pumping device is communicated, the flow path between the pumping device and the sampling end on the three-way sterile pipe is blocked, the collected water sample is injected into the sterile culture container through the pumping device, the collected water sample can be aseptically added into a culture medium at a sampling site, and the detection accuracy is high.

Further, sterile culture container includes that the top is by the sealed hyaline tube of rubber buffer and the blast pipe that is provided with the filtration gas, the culture medium is in the hyaline tube, the blast pipe set up in on the rubber buffer, the both ends of blast pipe communicate respectively the upper portion inner chamber and the atmosphere of hyaline tube, it is used for preventing the live bacteria in the atmosphere to pass through to strain the gas the blast pipe gets into the hyaline tube, the one end of aseptic pipe of tee bend has the syringe needle, the syringe needle inserts make in the rubber buffer the hyaline tube inner chamber with aseptic pipe of tee bend communicates.

The beneficial effect of adopting the further scheme is that: the design of syringe needle is convenient for the sealing connection between aseptic culture vessel and the tee bend aseptic tube, and before arriving the on-the-spot sampling, can dismantle and carry, it is more convenient to carry, and the blast pipe does benefit to the water sample and gets into in the hyaline tube.

Further, the side wall of the transparent tube is provided with a first scale.

The beneficial effect of adopting the further scheme is that: the culture medium and the amount of the water sample filled in the transparent tube can be conveniently mastered.

Further, still include disinfection spray bottle, disinfection spray bottle is used for advance the appearance needle insert before the rubber buffer right advance the appearance needle disinfect.

The beneficial effect of adopting the further scheme is that: the aseptic environment can be kept, and the aseptic environment in the transparent tube can be prevented from being damaged when the sampling needle is inserted into the rubber plug.

Further, the transparent tube is made of polyethylene.

The beneficial effect of adopting the further scheme is that: the material property is stable, and the transparent tube can be prevented from being damaged due to collision in the field environment.

Furthermore, the pumping and injecting device is a sterile injector, and the front end of the sterile injector is communicated with one end of the three-way sterile pipe.

The beneficial effect of adopting the further scheme is that: low cost and convenient operation.

Further, the side wall of the sterile syringe is provided with a second scale.

The beneficial effect of adopting the further scheme is that: the water sample collected and the amount of the water sample injected into the sterile culture container can be conveniently mastered.

Furthermore, the control device is a three-way valve, and the three-way valve is arranged at the intersection of each flow path of the three-way aseptic tube.

The beneficial effect of adopting the further scheme is that: the operation is convenient.

Further, the three-way sterile pipe is a hose.

The beneficial effect of adopting the further scheme is that: is convenient for storage, carrying and operation.

Further, the control device is a pipe clamp matched with a flow path of the three-way sterile pipe.

The beneficial effect of adopting the further scheme is that: low cost, simple structure and convenient manufacture.

Drawings

Fig. 1 is a schematic view of an embodiment of the present invention in which the control device is a three-way valve;

FIG. 2 is a schematic view of a suction device according to an embodiment of the present invention with the control device being a tube clamp;

FIG. 3 is a schematic view of a water sample being added to a sterile culture vessel by a suction device according to an embodiment of the present invention in which the control device is a tube clamp;

in the figure: 1. a sterile culture vessel; 11. a transparent tube; 12. a rubber plug; 13. a first scale; 14. a culture medium; 15. an exhaust pipe; 16. filtering gas; 2. a three-way sterile tube; 21. a sample injection needle; 22. a sampling needle; 3. a sterile syringe; 31. a second scale; 41. a three-way valve; 42. a pipe clamp; 5. a sterilizing spray bottle;

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings.

As shown in fig. 1-3, the embodiment of the microorganism sampling apparatus disclosed in the present invention comprises:

a sterile culture container 1 with a culture medium 14 in the inner cavity;

a pumping and injecting device for pumping the water sample and injecting the water sample in the sterile culture container 1;

wherein, both ends are respectively communicated with the sterile culture container 1 and the pumping and injecting device, the other end is used for a three-way sterile tube 2 for sampling, and the three-way sterile tube 2 is preferably a hose and can be a PVC hose;

and a control device for controlling the on-off of each flow path of the three-way sterile pipe 2.

Preferably, the sterile culture container 1 includes a transparent tube 11 sealed by a rubber plug 12 at the top and an exhaust tube 15 provided with a filtered gas 16, the culture medium 14 is in the transparent tube 11, the exhaust tube 15 is disposed on the rubber plug 12, two ends of the exhaust tube 15 are respectively communicated with an upper inner cavity of the transparent tube 11 and the atmosphere, the filtered gas 16 can be selected from 0.22 micron medical filtered gas, the filtered gas is disposed at an opening of the exhaust tube 15 communicated with the atmosphere, the filtered gas 16 is used for preventing viable bacteria in the atmosphere from entering the transparent tube 11 through the exhaust tube 15, the gas replaced in the transparent tube 11 can enter the atmosphere through the filtered gas 16, one end of the three-way sterile tube 2 is provided with a sample injection needle 21, the sample injection needle 21 is inserted into the rubber plug 12 to communicate the inner cavity of the transparent tube 11 with the three-way sterile tube 2, a sampling needle 22 can also be disposed at.

A transparent tube 11 having a negative pressure inside and sealed at the top by a rubber stopper 12 may also be used as the sterile culture container 1.

The transparent tube 11 can be a test tube made of polyethylene material, and the side wall of the transparent tube 11 is provided with a first scale 13.

As the further scheme of above-mentioned embodiment, still include disinfection spray bottle 5, disinfection spray bottle 5 is used for disinfecting injection needle 21 before injection needle 21 inserts rubber buffer 12, and disinfection spray bottle 5 can independently set up. Before each part arrives at the sampling site, the parts can be sterilized by ethylene oxide and then are aseptically packaged, the parts are carried by aseptic packaging bags during carrying, after arriving at the sampling site, the aseptic packaging bags are disassembled, and the parts such as the rubber plug 12, the sample injection needle 21 and the like are sterilized by the sterilizing spray bottle 5 and then are assembled.

Specifically, the suction and injection device is a sterile injector 3, the front end of the sterile injector 3 is communicated with one end of a three-way sterile tube 2, the front end of the sterile injector 3 can be directly welded with the three-way sterile tube 2, the front end of the sterile injector can also be connected through a joint, and the side wall of the sterile injector 3 is provided with second scales 31.

The specific control device may be a three-way valve 41, the three-way valve 41 is disposed at the intersection of the flow paths of the three-way aseptic tube 2, and the three-way valve 41 can control the on-off of the flow path between the aseptic culture container 1 and the suction device and the on-off of the flow path between the suction device and the sample injection end.

When the device is used, the sample introduction end is communicated with the pumping and injecting device through the three-way valve 41, the pumping and injecting device is disconnected with the sterile culture container 1, the pumping and injecting device collects a water sample from a water body and stores the water sample in the pumping and injecting device, after the required amount is reached, the pumping and injecting device is communicated with the sterile culture container 1 through the three-way valve 41, the sample introduction end is disconnected with the pumping and injecting device, the water sample in the pumping and injecting device is injected into the sterile culture container 1 for culture, after the water sample is injected, the sample introduction needle 21 is pulled out, and the sterile culture container 1 can be placed into a 37-degree incubator.

When the three-way aseptic tube 2 is a hose, the control device may be a pipe clamp 42 adapted to the flow path of the three-way aseptic tube 2.

When the device is used, the pipe clamp 42 is clamped on a flow path of the three-way sterile pipe 2 communicated with the sterile culture container 1 to block the flow path entering the sterile culture container 1, a pumping and injecting device is used for pumping a water sample into the pumping and injecting device through the sample introduction end, the pipe clamp 42 communicated with the flow path of the sterile culture container 1 is taken down after the water sample is pumped to a required amount, the pipe clamp 42 is clamped on the flow path of the three-way sterile pipe 2 communicated with the sample introduction end to block the flow path of the sample introduction end, the water sample collected in the pumping and injecting device is injected into the sterile culture container 1 through the pumping and injecting device, the sample introduction needle 21 is pulled out after the water sample injection is completed, and the sterile culture container 1 can be placed into a 37-degree.

Besides the sterile injector 3, the pumping device can also be selected from the existing pump devices with temporary storage cavities.

Of course, the present invention may have other embodiments, and those skilled in the art may make various corresponding changes and modifications according to the present invention without departing from the spirit and the essence of the present invention, and these corresponding changes and modifications should fall within the protection scope of the appended claims.

Claims (10)

1. A microorganism sampling apparatus, characterized by: the method comprises the following steps:

a sterile culture vessel (1) having a culture medium (14) in the interior thereof;

a pumping and injecting device for pumping a water sample and injecting the water sample in the sterile culture container (1);

wherein, two ends of the three-way aseptic tube (2) are respectively communicated with the aseptic culture container (1) and the pumping and injecting device, and the other end is used for sampling;

and the control device is used for controlling the on-off of each flow path of the three-way sterile pipe (2).

2. The microbial sampling device of claim 1, wherein: aseptic culture container (1) include the top by transparent pipe (11) that rubber buffer (12) are sealed and be provided with blast pipe (15) of straining gas (16), culture medium (14) are in transparent pipe (11), blast pipe (15) set up in on rubber buffer (12), the both ends of blast pipe (15) communicate respectively the upper portion inner chamber and the atmosphere of transparent pipe (11), strain gas (16) and be arranged in preventing the live bacteria in the atmosphere to pass through blast pipe (15) get into transparent pipe (11), the one end of tee bend aseptic tube (2) has and advances kind needle (21), advance kind needle (21) and insert make in rubber buffer (12) transparent pipe (11) inner chamber with tee bend aseptic tube (2) intercommunication.

3. The microbial sampling device of claim 2, wherein: the side wall of the transparent tube (11) is provided with a first scale (13).

4. The microbial sampling device of claim 2, wherein: still include disinfection spray bottle (5), disinfection spray bottle (5) are used for advance injection needle (21) to insert before rubber buffer (12) right advance injection needle (21) disinfect.

5. The microbial sampling device of claim 2, wherein: the transparent tube (11) is made of polyethylene.

6. The microbial sampling device of claim 1, wherein: the drawing and injecting device is a sterile injector (3), and the front end of the sterile injector (3) is communicated with one end of the three-way sterile pipe (2).

7. The microbial sampling device of claim 6, wherein: the side wall of the sterile syringe (3) is provided with a second scale (31).

8. The microbial sampling device of any one of claims 1-7, wherein: the control device is a three-way valve (41), and the three-way valve (41) is arranged at the intersection of each flow path of the three-way aseptic tube (2).

9. The microbial sampling device of any one of claims 1-7, wherein: the three-way sterile pipe (2) is a hose.

10. The microbial sampling device of claim 9, wherein: the control device is a pipe clamp (42) matched with a flow path of the three-way sterile pipe (2).

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