CN212207402U - Test strip for detecting blood sample - Google Patents

Abstract

The utility model provides a test strip for detecting blood sample, this test strip include that the sample applys the pad, have colored granule's mark bed course and porous film layer and the layer that absorbs water, wherein apply the sample that the layer includes the multilayer structure that absorbs water at the sample and apply the pad, set up some microchannels between two-layer application pad. These microchannels may be used to collect or block red blood cells from the blood sample, and the bibulous layer itself may absorb the fluid, thus reducing the blockage of red blood cells.

Description

Test strip for detecting blood sample

Technical Field

The utility model relates to a test strip, in particular to an immunity test strip for detecting blood samples.

Background

The following background is provided to aid the reader in understanding the present invention and is not admitted to be prior art.

The immunity test strip is a daily product for health condition detection in modern society, and is generally applied due to low cost and high detection speed. The test strip is an essential part of the in vitro rapid test. In immunoassays, a general test strip includes a sample application site, a labeling site of a colored particle site, and a porous membrane site and a water absorption site. Typically, the sample application site is glass fiber, the colored particles are latex or colloidal gold particles, and are treated on a polyester film, the colored particles are typically linked to an antibody or antigen, and the porous film is typically a nitrocellulose or nylon film, on which the antibody or antigen is treated. The immune test strip is made through liquid capillary force, and the test strip flows through the sample applying part and the mark part successively to dissolve the mark particle, flows to the film to react with the antibody on the film and finally stays in the water absorbing part. The reaction is generally completed in 2 to 3 minutes.

In a conventional test strip, one end of a sample application pad 106 is typically superimposed on one end of a marker particle pad 103, while the other end of the marker particle pad is superimposed on a porous film 100, such as a nitrocellulose film, while the other end of the porous film is superimposed on one end of a bibulous paper 101, such as the structure illustrated in FIG. 1. Test lines 104 and control lines 102 are typically included on the membrane. The marking pad has metal colloid or latex particles with color particles. However, the types of samples are various, and generally, blood samples, urine samples and saliva samples are used. The properties of each sample are different, and in particular, a blood sample, such as a whole blood sample, flows on a test strip, and the substance to be analyzed is often an antibody or an antigen, regardless of red blood cells, due to the presence of red blood cells and the like. While the red blood cells are in flow, the solution breaks down releasing substances that may interfere with the final reaction result. In addition, red blood cells generally tend to clog the sample pad, the label pad, the capillaries and the micropores of nitrocellulose, thereby causing clogging and affecting the flow of liquid.

In the conventional technology, the antibody against red blood cells is generally added to the sample pad to adsorb red blood cells, but the antibody is expensive, and in addition, an additional processing step is required, which increases the production cost. There is a need for improved structures for existing test strips that reduce the interference associated with red blood cell detection.

Disclosure of Invention

The utility model discloses a solve some problems of prior art, provide a new test strip structure, this test strip includes that the sample is applyed the pad, is had colored granule's mark bed course and porous film layer and the layer that absorbs water, wherein apply the sample that the layer includes multilayer water absorption structure at the sample and apply the pad, apply at two-layer and set up some microchannels between the pad, these microchannels can be used for collecting or block the red blood cell of blood sample, and the layer that absorbs water itself can adsorb liquid, has reduced the jam of red blood cell like this.

In some embodiments, the sample application pad comprises two absorbent layers, one on top of the other, with capillary channels formed between the absorbent layers. In some forms, the capillary channel has a dimension of between 0.5 and 4 millimeters. By utilizing the principle, although the water absorbing layer has capillary force, the size of the capillary tube is mostly between 0.1 and 0.5, and if a blood sample is dripped on the sample pad, the capillary tube can be blocked due to the accumulation of red blood cells, so that water molecules and small molecular substances cannot flow. And a plurality of capillary channels are arranged between the two layers, so that the red blood cells are positioned in the capillary channels, and the capillary force of the red blood cells and the capillary channels is equivalent to a filtering function, so that the red blood cells are positioned in the capillary channels, and the filtered solution, such as a serum sample, can easily flow on the sample pad. The capillary channel for filtering the red blood cells does not need to be manually arranged, but is used for filtering by means of a gap between two sample pads.

In some embodiments, a marker pad is held between two water absorbent layers, wherein one end of the marker layer is held by the two carriers and the other end of the marker layer is superposed with one end of the porous membrane layer.

In some modes, two water-absorbing layers are respectively provided with a hydrophilic waterproof film, and capillary channels for accommodating red blood cells are formed by means of the two hydrophilic waterproof films. In some embodiments, a water absorbent layer is disposed between two hydrophilic water impermeable films, the water absorbent layer being in contact with or superposed on the marking pad. This results in a red blood cell filter on the one hand and the filtered sample being guided through the water-absorbent layer to the sample pad on the other hand.

In some preferred forms, the sample pad comprises a plurality of sample pads, each sample pad having a capillary channel therebetween. Wherein the size of the capillary channel is larger than the size of the water-absorbing layer. When the sample pad is inserted into a blood sample, the sample first enters the capillary channel because the size of the capillary channel is larger than the capillary size of the absorbent pad itself, the filtered sample flows forward along the sample pad, and the filtered red blood cells stay in the capillary channel.

In another aspect, the present invention provides a test strip for testing a blood sample, the test strip comprising a test pad for absorbing the blood sample, a marker pad with colored particles, and a nitrocellulose membrane, wherein one end of the test pad is superimposed on one end of the marker pad, and the other end of the marker pad is superimposed on the nitrocellulose membrane, wherein the marker pad comprises a plurality of elongated water-absorbent materials, each two water-absorbent materials having a capillary channel therebetween for filling the blood sample, and the capillary channel has a capillary size larger than that of the water-absorbent materials.

In some preferred forms, the capillary passage has a diameter dimension of between 0.5 and 4 mm.

Advantageous effects

The utility model discloses improved the structural design of sample pad, not caught the erythrocyte with traditional chemical reagent, but adopted the mode of physical structure to go on, can hold back the erythrocyte in capillary passage for the flow of sample has reduced the interference of erythrocyte to the detection simultaneously.

Drawings

FIG. 1 is a schematic diagram of a prior art test strip configuration.

Fig. 2 is a schematic structural diagram of an embodiment of the present invention.

Fig. 3 is a schematic structural diagram of another embodiment of the present invention.

Fig. 4 is a schematic structural diagram of another embodiment of the present invention.

Fig. 5 is a schematic diagram for explaining the principle of structural design of another embodiment of the present invention.

Detailed Description

In some embodiments, such as that shown in FIG. 2, the sample pad has a two-layer structure, an upper layer 106-2 and a lower layer 106-1, with a capillary channel 109 therebetween, as seen in the figure, which is larger than the native capillary channel of the upper and lower layers. Generally, the upper and lower layers are made of dense glass cellulose filter paper, and the capillary channel is intentionally arranged on the basis of the upper and lower layers. The diversion layer is added at one end of the two layers, when the sample test strip is inserted into a blood sample, due to the fact that the capillary tubes are different in size, a part of a sample with red blood cells enters the capillary tube 109, and a part of the sample flows through the capillary force of the upper layer and the lower layer of the sample pad. These filtered samples flow through the channel strips 108 onto the marker pad 103, thus completing the corresponding test.

The strips 108 may be of the same material as the sample pad or of a different material, but the capillary dimensions of the material are much smaller than those of the capillary channel. For example, the conducting strips are made of dense filter paper. The size of the capillary channel is about 0.5-4 mm, preferably between 2-3 mm, but due to material constraints the capillary dimensions are typically small, much smaller than 0.5 mm. Such a configuration would naturally act as a filter for red blood cells, allowing the sample without red blood cells to flow into the label pad and then onto the nitrocellulose membrane, thereby completing the entire test.

In other embodiments, such as that shown in FIG. 3, a hydrophilic membrane 107.1,107-2 covers the sample pad between the upper and lower layers; a flow-guiding layer 108 is provided between the hydrophilic membranes. Therefore, the blood sample can rapidly flow into the capillary channel by means of the acting force of the capillary tube and the acting force of the hydrophilic layer. When these hydrophilic membranes are impervious to water, rely on conducting strip 108 to transmit liquid, in fact the effect of conducting strip 108 plays the effect of transmitting liquid one, also plays the effect of filtering simultaneously, and the capillary size of conducting strip is far less than the size of capillary channel 109, so, red blood cell generally is difficult to pass through the conducting strip, but serum etc. can pass through the conducting strip and transmit. The material of the conducting strip can be filter paper or a material which is compact in fiber and can absorb water.

In other embodiments, such as that shown in fig. 4, the absorbent pad is formed by a plurality of fine elongated absorbent materials distributed at the absorbent pad, and about 2-3 mm capillary channels (capillary gaps) are left between every two fine absorbent pads, and the gaps are opened at one end and blocked by the transversely arranged marking pad at the other end. When the absorbent pad end of the test strip is inserted into a blood sample, the blood sample rapidly enters the slit because the size of the slit is larger than the capillary size of the absorbent material, part of the blood sample continuously flows through the absorbent material, the blood sample entering the slit simultaneously receives capillary force and moves on the fine and long absorbent material, and red blood cells basically stay in the long and long slit and are filtered. Thus, the filtered red blood cells do not substantially flow onto the label pad. The material of general mark pad and the material of water-absorbing material are similar, and are all compacter, when the red blood cell moves in these compact materials, can block capillary duct, move slowly in these compact materials like this to just can filter the red blood cell in the gap. The specific principle is explained in figure 5. When a blood sample enters a capillary gap and flows through the water absorbing material, the size of the capillary gap is larger than the property of the water absorbing material, so that the capillary gap can be distinguished from the property of the water absorbing material, the blood sample can move faster in the common gap, the whole gap is filled with the faster blood sample, the area contacting the water absorbing material is increased easily, more samples can enter the water absorbing materials 1061 and 106-3, even if red blood cells can block a capillary channel, the area is increased, more red blood cells can be intercepted outside, and the liquid sample filtered by the red blood cells can enter the water absorbing material, so that the liquid amount required by the flow of a test strip is met, and the detection and the assay can be completed smoothly.

The invention shown and described herein may be practiced in the absence of any element or elements, limitation or limitations, which is specifically disclosed herein. The terms and expressions which have been employed are used as terms of description and not of limitation, and there is no intention in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, and it is recognized that various modifications are possible within the scope of the invention. It should therefore be understood that although the present invention has been specifically disclosed by various embodiments and optional features, modification and variation of the concepts herein described may be resorted to by those skilled in the art, and that such modifications and variations are considered to be within the scope of this invention as defined by the appended claims.

The contents of the articles, patents, patent applications, and all other documents and electronically available information described or cited herein are hereby incorporated by reference in their entirety to the same extent as if each individual publication was specifically and individually indicated to be incorporated by reference. Applicants reserve the right to incorporate into this application any and all materials and information from any such articles, patents, patent applications, or other documents.

Claims (8)

1. A test strip for testing a blood sample, the test strip comprising a test pad for absorbing the blood sample, a marker pad with colored particles, and a nitrocellulose membrane, wherein one end of the test pad is superimposed on one end of the marker pad, and the other end of the marker pad is superimposed on the nitrocellulose membrane, characterized in that the marker pad comprises a capillary channel for filling the blood sample, the capillary channel having a capillary size larger than that of the marker pad.

2. The test strip of claim 1, wherein the marker pad comprises upper and lower layers of absorbent material, the upper and lower layers of absorbent material defining the capillary channel therebetween, the capillary channel having a capillary dimension greater than the capillary dimensions of the upper and lower layers of absorbent material.

3. The test strip of claim 2, wherein a wicking strip is positioned between the upper and lower absorbent materials for wicking fluid to the marker pad.

4. The test strip of claim 3, wherein each of the upper and lower layers of hydrophilic material is covered with a water-impermeable affinity layer, and the flow-directing strip is positioned between the two hydrophilic layers.

5. The test strip of any one of claims 1-4, wherein the capillary channel has a diameter dimension of 0.5-4 mm.

6. A test strip for testing a blood sample, the test strip comprising a test pad for absorbing the blood sample, a marker pad with colored particles, and a nitrocellulose membrane, wherein one end of the test pad is superimposed on one end of the marker pad, and the other end of the marker pad is superimposed on the nitrocellulose membrane, characterized in that the marker pad comprises a plurality of elongated bibulous materials, each two bibulous materials having a capillary channel therebetween for filling the blood sample, the capillary channel having a capillary dimension greater than that of the bibulous materials.

7. The test strip of claim 6, wherein the capillary channel has a diameter dimension of 0.5-4 mm.

8. The test strip of claim 7, wherein the bibulous material is a filter paper material.

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