CN212199262U - Germ detecting instrument based on laser spectrum technology - Google Patents

Abstract

The utility model provides a germ detecting instrument based on laser spectrum technique, including pulse generator, pulse generator triggers laser instrument transmission ultraviolet laser, is equipped with coupling lens, Y type optic fibre, light filter and beam splitting system along the light path in proper order, and wherein ultraviolet laser gets into the transmitting terminal of Y type optic fibre through the coupling lens coupling, through the fluorescence signal that ultraviolet laser arouses the liquid to be measured and produce is received to the probe of Y type optic fibre, and this fluorescence signal incides the light filter through the receiving terminal of Y type optic fibre, and the light signal after the filtering processing incides beam splitting system, and the light signal after the beam splitting carries out photoelectric signal by the image intensifier and enlargies, the image intensifier is connected with the high-voltage source, by the high-voltage source provides high-voltage power source, the high-voltage source is controlled by pulse generator, the image intensifier is connected with CCD, and spectral signal formation of image is on the CCD surface, the CCD is connected with a signal processing system. The detection instrument has the advantages of small volume, portability, high detection speed and the like.

Description

Germ detecting instrument based on laser spectrum technology

Technical Field

The utility model relates to a germ detecting instrument technical field especially relates to a germ detecting instrument based on laser spectroscopy technique.

Background

At present, a rapid detection method for viruses and bacteria has become a hot point of research, and the current real-time fluorescence quantitative PCR nucleic acid detection method can be applied to the detection of bacteria and viruses, but has the following disadvantages: the whole operation process is complicated, long in time consumption, needs to be operated by professional personnel, needs to be subjected to centralized inspection, and the detection result is easily influenced by various factors.

The fluorescence spectroscopy has the advantages of high sensitivity, convenience and quickness in detection and the like, and is widely applied to detection of bacteria and viruses, but when toxic microorganisms are detected by adopting an instrument for detecting germs by adopting the fluorescence spectroscopy in the prior art, the toxic microorganisms are small in size and low in microorganism content in a detection sample, so that a fluorescence signal is weak, and a more accurate detection result cannot be achieved.

SUMMERY OF THE UTILITY MODEL

In order to solve the problem that exists among the prior art, the utility model provides a germ detecting instrument based on laser spectrum technique, it has advantages such as small, portable, detection speed is fast, can be used for harmful microorganism's on-the-spot short-term test.

In order to achieve the above object, the utility model provides a germ detecting instrument based on laser spectrum technology, including pulse generator, pulse generator triggers laser instrument transmission ultraviolet laser, is equipped with coupling lens, Y type optic fibre, light filter and beam splitting system along the light path in proper order, and wherein ultraviolet laser gets into the transmitting terminal of Y type optic fibre through the coupling lens coupling, receives the fluorescence signal that ultraviolet laser arouses the liquid to be measured and produce through the probe of Y type optic fibre, and this fluorescence signal incides the light filter through the receiving terminal of Y type optic fibre, and the light signal after the filtering processing incides beam splitting system, and the light signal after the beam splitting is carried out photoelectric signal by the image intensifier and is enlargied, the image intensifier is connected with the high-pressure source, by the high-pressure source provides the high-pressure power supply, the high-pressure source is controlled by pulse generator, the image intensifier is connected with CCD, the spectrum signal is imaged on the surface of the CCD, and the CCD is connected with a signal processing system.

The utility model has the advantages that the germ detecting instrument based on the laser spectrum technology has the advantages of small volume, portability, high detecting speed and the like, and can be used for the on-site rapid detection of harmful microorganisms; ultraviolet laser with proper wavelength is selected to excite different microorganisms to generate characteristic fluorescence spectra, the detection instrument is adopted to detect, and the types of the microorganisms can be identified through spectral analysis.

Drawings

Fig. 1 shows a schematic structural diagram of a pathogen detection apparatus based on laser spectroscopy according to the present invention.

Reference numerals: 1-pulse generator, 2-laser, 3-coupling lens, 4-Y type optical fiber, 5-optical filter, 6-light splitting system, 7-high voltage source, 8-image intensifier, 9-CCD, 10-signal processor and A-liquid to be measured.

Detailed Description

The following description will further describe embodiments of the present invention with reference to the accompanying drawings.

As shown in fig. 1, the pathogen detection apparatus based on laser spectroscopy technology of the present invention comprises a pulse generator 1, wherein the pulse generator 1 triggers a laser 2 to emit ultraviolet laser, a coupling lens 3, a Y-shaped optical fiber 4, an optical filter 5 and a light splitting system 6 are sequentially disposed along a light path, wherein the ultraviolet laser enters a transmitting end of the Y-shaped optical fiber 4 through the coupling lens 3, a probe of the Y-shaped optical fiber 4 detects a liquid a to be detected, specifically, the liquid a to be detected can be in a test tube, on a flat plate or on other carriers, the probe of the Y-shaped optical fiber 4 receives a fluorescence signal generated by exciting the liquid a to be detected by the ultraviolet laser, the fluorescence signal is incident to the optical filter 5 through a receiving end of the Y-shaped optical fiber 4 for light filtering treatment, then incident to the light splitting system 6 for light splitting, and an optical signal after light splitting is amplified by an image intensifier 8, the image intensifier 8 is connected with a high-voltage source 7, the high-voltage source 7 provides high-voltage power, the high-voltage source 7 is controlled by the pulse generator 1, namely the pulse generator 1 triggers the image intensifier 8 to work, the image intensifier 8 is connected with a CCD9, a spectrum signal is imaged on the surface of a CCD9, and the CCD9 is connected with a signal processing system 10, and the spectrum signal is input into the signal processing system 10 to be processed so as to detect spectrum information for identifying germs.

In order to improve fluorescence intensity, increase fluorescence detection sensitivity, the utility model discloses mainly adopt two kinds of measures to improve instrument detection performance, firstly laser source adopts ultraviolet laser, and laser is not only monochromatic good, and intensity is far more than light sources such as ultraviolet LED, ultraviolet lamp moreover, has consequently increased fluorescence intensity. In addition, the low-light-level image intensifier is used as a spectrum detector, and the sensitivity of the detection instrument is greatly improved through synchronous time sequence control of the laser pulse and the image intensifier.

The utility model relates to a germ detecting instrument based on laser spectrum technology, which has the advantages of small volume, portability, high detecting speed and the like, and can be used for the on-site rapid detection of harmful microorganisms; ultraviolet laser with proper wavelength is selected to excite different microorganisms to generate characteristic fluorescence spectra, the detection instrument is adopted to detect, and the types of the microorganisms can be identified through spectral analysis.

At present, the research work of inducing the biological substance to generate the fluorescence spectrum by using laser (especially ultraviolet laser) has achieved related results, and the wavelength range of the fluorescence spectrum of the biological substance is mainly 300-800 nm. Therefore, by selecting a proper ultraviolet laser wavelength, high-efficiency fluorescence excitation of the virus sample can be realized, and currently selectable laser excitation wavelengths comprise: 266nm, 355nm, 405nm, 450nm, etc. The fluorescence spectrum of an organic substance having a single component does not change due to the wavelength of the excitation light; in the case of more complex biological mixtures, such as fungi, bacteria, viral pollen, spores, etc., these are composed of fluorescent molecules such as coenzymes, tryptophan, riboflavin, tyrosine, etc., which differ greatly in their proportions and structures. Therefore, the fluorescence spectrum of the mixed organic substance is obtained by superimposing fluorescence spectra generated by various fluorescent molecules.

Claims (1)

1. A germ detecting instrument based on laser spectrum technology is characterized in that: the device comprises a pulse generator, wherein the pulse generator triggers a laser to emit ultraviolet laser, a coupling lens, a Y-shaped optical fiber, an optical filter and a light splitting system are sequentially arranged along a light path, the ultraviolet laser enters the emitting end of the Y-shaped optical fiber through the coupling lens in a coupling mode, a probe of the Y-shaped optical fiber receives a fluorescent signal generated by exciting liquid to be detected by the ultraviolet laser, the fluorescent signal enters the optical filter through the receiving end of the Y-shaped optical fiber, an optical signal after optical filtering processing enters the light splitting system, the optical signal after optical splitting is subjected to photoelectric signal amplification by an image intensifier, the image intensifier is connected with a high-voltage source, the high-voltage source is provided with a high-voltage power source, the high-voltage source is controlled by the pulse generator, the image intensifier is connected with a CCD, a spectrum signal is imaged on the surface of the CCD, and the CCD.

Images