CN212077070U - Draw liquid system and nucleic acid extraction device add - Google Patents

Abstract

The utility model provides a liquid adding and pumping system, which comprises a reagent conveying device, a liquid pumping control device, a liquid adding and pumping arm and a liquid adding and pumping arm transfer device, wherein the liquid adding and pumping arm transfer device is used for driving the liquid adding and pumping arm to move along the horizontal direction and the vertical direction; each reagent dropper is respectively communicated with a reagent conveying device, and the reagent conveying device is used for conveying an extraction reagent to each reagent dropper, wherein the extraction reagent is a cracking solution, a binding solution, a rinsing solution and an eluent; each suction head is respectively communicated with a liquid pumping control device which is used for controlling each suction head to pump waste liquid. A nucleic acid extraction device comprises the liquid adding and extracting system. The utility model provides a with drawing liquid system not only can add various reagent that draw to the reaction tube, can take away the waste liquid after the reaction in the reaction tube simultaneously for nucleic acid extraction element's work efficiency has improved.

Description

Draw liquid system and nucleic acid extraction device add

Technical Field

The utility model belongs to the technical field of nucleic acid extraction, more specifically say, relate to a add drawing liquid system and nucleic acid extraction element.

Background

Along with the popularization of gene detection, personalized drug delivery, prenatal diagnosis and the like, the traditional DNA extraction method is more and more obviously limited today when high flux and automation are pursued in various fields of the biological industry. Because the magnetic bead method for extracting nucleic acid can realize automatic extraction and large-scale operation, and has simple operation and short time, the magnetic bead method for extracting nucleic acid is more and more emphasized.

Generally, the extraction steps for extracting nucleic acid by the magnetic bead method mainly comprise: (1) cracking; (2) combining; (3) rinsing; (4) and (4) eluting. Specifically, the DNA/RNA is released from cells or tissues under the action of a lysis solution, and the surface-modified superparamagnetic silica nano magnetic beads are specifically combined with the released DNA/RNA to form a nucleic acid-magnetic bead compound. Adding a rinsing liquid into the nucleic acid-magnetic bead compound, washing off impurities such as non-specifically adsorbed protein and polysaccharide, separating the nucleic acid-magnetic bead compound with the impurities removed from the magnetic bead in an eluent, and finally obtaining a nucleic acid substance to be extracted. If the steps of cracking, combining, rinsing and eluting are not mixed uniformly in the nucleic acid extraction process, the reagent is not fully contacted with the sample, the intracellular nucleic acid is not completely released, the magnetic beads are easy to settle, and the extraction efficiency is greatly reduced.

At present, a plurality of extraction steps of nucleic acid extraction by a magnetic bead method are carried out in different reaction tubes, so that the nucleic acid extraction of each sample needs to be provided with a plurality of reaction tubes, if a plurality of samples need to be simultaneously subjected to nucleic acid extraction, more reaction tubes need to be arranged, and due to the increase of the number of the reaction tubes, more nucleic acid extraction work cannot be carried out at one time, so that the nucleic acid extraction efficiency is reduced, the cost is high, and the plastic consumables have pressure on environmental protection.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a add drawing liquid system to the nucleic acid extraction of solving every sample that exists among the prior art need set up a plurality of reaction tubes and lead to carrying out the inefficiency of a plurality of sample nucleic acid extraction work simultaneously and with high costs technical problem.

In order to achieve the above object, the utility model adopts the following technical scheme: the liquid adding and extracting system comprises a reagent conveying device, a liquid extracting control device, a liquid adding and extracting arm and a liquid adding and extracting arm transfer device, wherein the liquid adding and extracting arm transfer device is used for driving the liquid adding and extracting arm to move along the horizontal direction and the vertical direction; each reagent dropper is respectively communicated with the reagent conveying device, and the reagent conveying device is used for conveying an extraction reagent to each reagent dropper, wherein the extraction reagent is a lysis solution, a binding solution, a rinsing solution and an eluent; and each suction head is respectively communicated with the liquid pumping control device, and the liquid pumping control device is used for controlling each suction head to pump waste liquid.

Optionally, the reagent conveying device includes a plurality of reagent bottles and a liquid path conveying assembly, different extraction reagents are respectively stored in each reagent bottle, input ports of the liquid path conveying assembly are respectively connected with each reagent bottle in a one-to-one correspondence manner, an output port of the liquid path conveying assembly is respectively connected with each reagent dropper through a multi-way pipe, the liquid path conveying assembly is used for branching and conveying the reagent in each reagent bottle to each reagent dropper according to a preset manner, and the liquid adding and extracting arm transfer device is used for driving each reagent dropper to move so as to drop each reagent into the reaction tube.

Optionally, the liquid path conveying assembly includes a first liquid path plate and a plurality of second liquid path plates, each of the second liquid path plates is sequentially stacked along a first direction, the first liquid path plate is respectively attached to and connected with each of the second liquid path plates along a second direction, and the first direction is perpendicular to the second direction; each input port is arranged on the first liquid circuit board and distributed along the first direction, each output port is distributed along the second direction, each second liquid circuit board is provided with two output ports, each input port can be respectively communicated with each output port, and the output ports are mutually communicated in each second liquid circuit board.

Optionally, a plurality of liquid inlet holes and a plurality of first connecting holes are formed in the first liquid circuit board, a plurality of second connecting holes and a plurality of third connecting holes are formed in the second liquid circuit board, the input port is arranged at one end of the liquid inlet hole, the other end of the liquid inlet hole is connected with one end of the first connecting hole through a first injection pump, the other end of the first connecting hole is connected with one end of the second connecting hole through a first liquid channel, the first liquid channel extends along the second direction, the other end of the second connecting hole is connected with one end of the third connecting hole through a second injection pump, the other end of the third connecting hole is connected with the output port, a normally closed first electromagnetic valve is respectively arranged on each first liquid channel, the second connecting holes are sequentially communicated, and the third connecting holes are sequentially communicated.

Optionally, the liquid suction control device includes air pipes respectively communicated with the suction heads, and liquid suction motors respectively connected with the air pipes and used for controlling the suction heads to form negative pressure to draw waste liquid, and second electromagnetic valves are respectively arranged in the air pipes.

Optionally, the pumping arm adding and transferring device comprises a horizontal conveying mechanism and a lifting mechanism arranged on the horizontal conveying mechanism in a sliding manner, and the pumping arm adding is arranged at the output end of the lifting mechanism.

Optionally, the horizontal conveying mechanism and the lifting mechanism are both belt conveying mechanisms.

Optionally, add the drawing liquid arm including install in elevating system goes up and along the connecting plate of vertical direction extension, and install in the mount pad of connecting plate bottom, seted up one row of first mounting hole and one row of second mounting hole on the mount pad, first mounting hole with the second mounting hole sets up side by side, first mounting hole is used for installing the reagent burette, the second mounting hole is used for installing the suction head.

Optionally, install the connector in the second mounting hole, the below of mount pad is equipped with the push jack, the bottom of connector is followed the bottom surface of push jack is worn out, the suction head cover is located be located on the connector the position of push jack below, the push jack can move down in order to incite somebody to action the suction head is followed push up down on the connector.

The utility model also provides a nucleic acid extraction element, including the above-mentioned drawing liquid system that adds.

The utility model provides a with drawing liquid system's beneficial effect lies in: compared with the prior art, the utility model discloses an add the drawing liquid system and include reagent conveyor, drawing liquid controlling means, add the drawing liquid arm and add drawing liquid arm transfer device, make and to add the drawing liquid system and not only can add the extraction reagent to the reaction tube, waste liquid after the reaction can be taken away in the reaction tube simultaneously, thus, make the work of drawing of a nucleic acid can independently be accomplished to same reaction tube, the quantity of reaction tube is the quantity that this nucleic acid extraction device can carry out nucleic acid extraction simultaneously promptly, thereby make this nucleic acid extraction device can carry out the quantity of nucleic acid extraction work simultaneously more, and then improved nucleic acid extraction device's work efficiency. Meanwhile, each reagent dropper and each sucker are integrated on the same liquid adding and pumping arm, and the liquid adding and pumping arm is driven to move by the same liquid adding and pumping arm transfer device, so that the whole liquid adding and pumping system is optimized and simple in structure, small in occupied space and low in cost. In addition, the nucleic acid extraction device provided by the embodiment has higher nucleic acid extraction efficiency through the arrangement of the liquid adding and extracting system.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the embodiments or the prior art descriptions will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive labor.

FIG. 1 is a schematic structural view of a nucleic acid extraction apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic view of the fluid transport assembly of FIG. 1;

FIG. 3 is a schematic structural view of the first and second fluid plates in FIG. 2;

FIG. 4 is a schematic structural view of the fluid pumping arm transfer device shown in FIG. 1;

FIG. 5 is a schematic view of the fluid pumping arm of FIG. 1;

figure 6 is another perspective view of the fluid application arm of figure 5.

Wherein, in the figures, the respective reference numerals:

10-a reagent delivery device; 20-a liquid extraction control device; 30-adding a liquid pumping arm; 40-adding a liquid pumping arm transfer device; 50-reagent dropper; 60-a suction head; 70-a base frame; 11-a liquid path conveying assembly; 31-a connecting plate; 32-a mounting seat; 33-a push sheet; 34-a second drive motor; 35-a screw rod; 41-horizontal conveying mechanism; 42-a lifting mechanism; 43-a mounting frame; 71-a mounting plate; 111-a first fluid pathway plate; 112-a second liquid circuit board; 113-a first syringe pump; 114-a second syringe pump; 115-a first solenoid valve; 321-a first mounting hole; 322-a second mounting hole; 323-connecting head; 331-a third mounting hole; 411 — first drive motor; 412-a drive wheel; 413-a conveyor belt; 414-driven wheel; 415-a guide rail; 1110-an input port; 1111-liquid inlet hole; 1112-a first connection hole; 1113-first fluid channel; 1114 — a first via; 1120-an output port; 1121 — second connection hole; 1122-third connecting hole; 1123-a second fluid passage; 1124-screw hole; 200-reaction tube.

Detailed Description

In order to make the technical problem, technical solution and advantageous effects to be solved by the present invention more clearly understood, the following description is given in conjunction with the accompanying drawings and embodiments to illustrate the present invention in further detail. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.

It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in an orientation or positional relationship indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

Referring to fig. 1, a fluid pumping system according to the present invention will now be described. The liquid adding and extracting system is used in a nucleic acid extracting device, and is specifically used for conveying a sample reagent and an extraction reagent to the reaction tube 200 and extracting waste liquid after reaction in the reaction tube 200.

The liquid adding and extracting system comprises a reagent conveying device 10, a liquid extracting control device 20, a liquid adding and extracting arm 30 and a liquid adding and extracting arm transfer device 40, wherein the liquid adding and extracting arm transfer device 40 is used for driving the liquid adding and extracting arm 30 to move along the horizontal direction and the vertical direction, eight reagent droppers 50 and eight suction heads 60 are arranged on the liquid adding and extracting arm 30, and the number of the reagent droppers 50 is the same as that of the suction heads 60. It is understood that in other embodiments of the present invention, the number of the reagent droppers 50 and the pipette tips 60 may be five, six, seven or more than seven, which is not limited herein.

Each reagent dropper 50 is respectively communicated with the reagent conveying device 10, and the reagent conveying device 10 is used for conveying extraction reagents to each reagent dropper 50, wherein the extraction reagents are lysis solution, binding solution, rinsing solution and eluent. Specifically, the sample reagent is a cell or tissue solution, the extraction reagent is a lysis solution, a binding solution, a rinsing solution or an eluent, the lysis solution is used for releasing (nucleic acid) DNA/RNA in the cell or tissue solution through lysis, the binding solution is used for binding magnetic beads and nucleic acid, the rinsing solution is used for washing impurities such as nonspecifically adsorbed proteins and polysaccharides in the nucleic acid, the nucleic acid can be washed only after 1-3 times of washing, and the eluent is used for eluting the nucleic acid from the magnetic beads to complete the extraction of the nucleic acid.

In actual operation, the extracting reagent is transferred to each reagent dropper 50 by the reagent transferring device 10, the pipetting arm 30 is driven by the pipetting arm transferring device 40 to move in the horizontal direction and the vertical direction until the pipetting arm 30 is positioned right above each reaction tube 200, and then each reagent dropper 50 is controlled by the reagent transferring device 10 to add the extracting reagent into each reaction tube 200.

The suction heads 60 are respectively communicated with the liquid suction control device 20, and the liquid suction control device 20 is used for controlling the suction heads 60 to suck waste liquid. During specific work, the liquid adding and extracting arm 30 is driven by the liquid adding and extracting arm transfer device 40 to move along the horizontal direction and the vertical direction until the liquid adding and extracting arm 30 is positioned right above each reaction tube 200, and then each suction head 60 is controlled by the liquid extracting control device 20 to extract waste liquid after reaction in the reaction tube 200.

The utility model provides an add drawing liquid system includes reagent conveyor 10, drawing liquid controlling means 20, add drawing liquid arm 30 and add drawing liquid arm transfer device 40, make this add drawing liquid system not only can add in to reaction tube 200 and draw reagent, waste liquid after the reaction can be taken away in the reaction tube 200 simultaneously, thus, make the work of drawing of a nucleic acid can independently be accomplished to same reaction tube 200, the quantity of reaction tube 200 is the quantity that this nucleic acid extraction device can carry out nucleic acid extraction simultaneously promptly, thereby make this nucleic acid extraction device can carry out the quantity of nucleic acid extraction work simultaneously more, and then improved nucleic acid extraction device's work efficiency. Meanwhile, the reagent droppers 50 and the suckers 60 are integrated on the same liquid adding and extracting arm 30, and the liquid adding and extracting arm 30 is driven to move by the same liquid adding and extracting arm transfer device 40, so that the whole liquid adding and extracting system is optimized and simple in structure, small in occupied space and low in cost.

In this embodiment, referring to fig. 1 and fig. 2, the reagent conveying system 10 includes five reagent bottles (not shown) and a liquid path conveying assembly 11, wherein each reagent bottle stores an extraction reagent, the liquid path conveying assembly 11 has six input ports 1110 and six output ports 1120, the input ports 1110 of the liquid path conveying assembly 11 are correspondingly connected to each reagent bottle, respectively, the output ports 1120 of the liquid path conveying assembly 11 are respectively connected to each reagent dropper 12 through a multi-way pipe, specifically, the multi-way pipe is an eight-way pipe, which has eight liquid outlets, and the eight liquid outlets are correspondingly connected to the eight reagent droppers 12, respectively. In this embodiment, five extraction reagents are required according to the extraction requirements, so only five of the input ports are selected, while in other embodiments, four or six input ports may be selected according to the actual requirements. In addition, in the present embodiment, eight reagent dripping nozzles 12 are required, and therefore, the respective output ports 1120 are respectively communicated with the respective reagent dripping nozzles 12 through the octant tubes, while in other embodiments, the types of the multi-channel tubes may be changed according to the number of the reagent dripping nozzles 12, which is not limited herein.

The liquid path conveying assembly 11 is used for conveying the reagent in each reagent bottle to each reagent dropper 12 in a branching manner according to a preset mode, and the liquid adding arm transfer device 40 is used for driving each reagent dropper 12 to move so as to drop each reagent into the reaction tube. Therefore, the reagent bottle can be controlled to be respectively conveyed to the eight reagent droppers 12 in the same time through the same liquid path conveying assembly 11, the reagent is added into the reaction tubes through the reagent droppers 12, then the reagent droppers 12 are driven to horizontally move through the liquid adding and pumping arm transfer device 40, liquid adding is sequentially completed for the forty-eight reaction tubes, and the liquid adding device is simple in structure. It is understood that in other embodiments of the present application, the number of reagent bottles, the number of input ports 1110 and the number of output ports 1120, such as five or seven reagent bottles, are correspondingly adjusted according to the number of reagents to be added, and are not limited herein.

In this embodiment, referring to fig. 3, the liquid path conveying assembly 11 includes a first liquid path plate 111 and three second liquid path plates 112, each of the second liquid path plates 112 is sequentially stacked along a first direction X, the first liquid path plate 111 is respectively attached to each of the second liquid path plates 112 along a second direction Y, the first direction X is perpendicular to the second direction Y, specifically, the first direction X is a vertical direction in fig. 3, and the second direction Y is a horizontal direction in fig. 3. Each input port 1110 is opened on the first liquid path plate 111 and distributed along the first direction X, each output port 1120 is distributed along the second direction Y, and each second liquid path plate 112 is opened with two output ports 1120, each input port 1110 can be respectively communicated with each output port 1120, and each output port 1120 is communicated with each other in each second liquid path plate 112. In this embodiment, the first liquid channel plate 111 and the three second liquid channel plates 112 are fixed in a mutually abutting manner, and the first liquid channel plate 111 and the second liquid channel plate 112 are provided with holes to form branch conveying of liquid channels, so that a long hole extending from the first liquid channel plate 111 to the second liquid channel plate 112 can be divided into a two-section short hole connection mode, and a long hole communicating with each output port 1120 can be divided into three-section short holes to be connected, so that the problem that the long hole is difficult to drill can be solved, the processing difficulty of the liquid channel conveying assembly 11 is reduced, the processing efficiency and the yield of the liquid channel conveying assembly 11 are improved, and further the efficiency of nucleic acid extraction is improved. It is understood that in other embodiments of the present application, the number of the second liquid path plates 112 may also be adjusted according to the number of the output ports 1120, as long as two output ports 1120 are ensured on each second liquid path plate 112, which is not limited herein.

In the embodiment, please refer to fig. 3, six liquid inlet holes 1111 and six first connection holes 1112 are formed on the first liquid channel plate 111, and six second connection holes 1121 and six third connection holes 1122 are formed on the second liquid channel plate 112, wherein the number of the input ports 1110, the liquid inlet holes 1111, the reagent bottles, the first connection holes 1112 and the second connection holes 1121 is the same, the number of the third connection holes 1122 and the output ports 1120 is the same, the liquid inlet holes 1111 penetrate through the first liquid channel plate 111 along a third direction, the first connection holes 1112, the second connection holes 1121 and the third connection holes 1122 are blind holes extending along the third direction, and the openings are disposed at a side away from the reagent bottles, specifically, the first direction X, the second direction Y and the third direction are perpendicular to each other, and the third direction is the front-back direction in fig. 6. It is understood that in other embodiments of the present invention, the number of the input ports 1110, the liquid inlet holes 1111, the reagent bottles, the first connecting holes 1112 and the second connecting holes 1121 may be correspondingly changed along with the number of the reagent bottles, and the number of the third connecting holes 1122 and the output ports 1120 may also be correspondingly changed according to the number of the reaction tubes in each row, which is not limited herein.

The input port 1110 is disposed at one end of the liquid inlet hole 1111, the other end of the liquid inlet hole 1111 is connected to one end of the first connection hole 1112 through the first syringe pump 113, the other end of the first connection hole 1112 is connected to the first liquid channel 1113 through one end of the second connection hole 1121, the first liquid channel 1113 extends along the second direction Y, the other end of the second connection hole 1121 is connected to the second syringe pump 114 through one end of the third connection hole 1122, the other end of the third connection hole 1122 is connected to the output port 1120, specifically, the other end of the third connection hole 1122 is connected to the output port 1120 through the second liquid channel 1123, and the second liquid channel 1123 extends along the second direction Y. Each first fluid passage 1113 is provided with a normally closed first solenoid valve 115, and each second connection hole 1121 is sequentially communicated along the first direction X, and each third connection hole 1122 is sequentially communicated along the first direction X. In a specific operation, when the reagent in a certain reagent bottle needs to be delivered to each reagent dropper 12, the first electromagnetic valve 115 corresponding to the reagent bottle needs to be opened, and the corresponding injection pump is started, so that the reagent in the reagent bottle can be sequentially delivered to each reagent dropper 12 through the first liquid channel 1113 and the second liquid channel 1123.

Referring to fig. 3, the first liquid path plate 111 is provided with a first through hole 1114, the first through hole 1114 penetrates through the first liquid path plate 111 along the second direction Y, each second liquid path plate 112 is provided with a screw hole 1124, the screw holes 1124 extend along the second direction Y and open towards the first liquid path plate 111, the first liquid path plate 111 and each second liquid path plate 112 are locked by bolts, the bolts sequentially pass through the first through hole 1114 and the screw holes 1124 and are locked, a gasket is supported between the first liquid path plate 111 and the second liquid path plate 112, and the gasket is sleeved on the bolts, so that the first liquid path 1113 is sealed, and the reagent in the first liquid path 1113 is prevented from leaking out between the first liquid path plate 111 and the second liquid path plate 112. In addition, the first through hole 1114 and the first liquid passage 1113 are arranged in a staggered manner in the first direction X.

Similarly, the second fluid plates 112 are fastened together by fasteners, such as the fasteners sequentially penetrate through the second fluid plates 112 in the first direction X and are fastened together.

Referring to fig. 2, each first electromagnetic valve 115 is mounted on a side of the first fluid path plate 111 away from the reagent bottle, each first syringe pump 113 is mounted on a side of the first fluid path plate 111 away from the reagent bottle and locked by a fastener, and each second syringe pump 114 is mounted on a side of the second fluid path plate 112 away from the fluid storage tube and locked by a fastener.

In this embodiment, the liquid pumping control device 20 includes a liquid pumping motor (not shown) and eight air pipes, the number of the air pipes is the same as that of the suction heads 60, each air pipe is respectively communicated with each suction head 60, the liquid pumping motor is respectively connected with each air pipe, and the liquid pumping motor is used for controlling each air pipe and each suction head 60 to form negative pressure, and enabling each suction head 60 to respectively pump waste liquid generated after reaction in different reaction tubes 200. In addition, a second electromagnetic valve is respectively arranged in each air pipe, the second electromagnetic valve is a normally open electromagnetic valve, and the communication state of each air pipe and the suction head 60 can be controlled through the second electromagnetic valve, so that whether the suction head 60 can suck waste liquid or not is controlled.

Referring to fig. 4, in the present embodiment, the pipetting arm transferring apparatus 40 includes a horizontal conveying mechanism 41 and a lifting mechanism 42, the horizontal conveying mechanism 41 is mounted on the base frame 70, the lifting mechanism 42 is slidably disposed on the horizontal conveying mechanism 41, the pipetting arm 30 is mounted at an output end of the lifting mechanism 42, each pipette tip 60 and each reagent pipette 50 are respectively mounted on the pipetting arm 30, the horizontal conveying mechanism 41 is configured to horizontally move the lifting mechanism 42, each pipette tip 60 and each reagent pipette 50 as a whole, the lifting mechanism 42 is configured to convey each pipette tip 60 and each reagent pipette 50 to or out of the reaction tube 200, the number of pipette tips 60, the number of reagent pipette tips 50 and the number of reaction tubes 200 in each row are equal, the pipette tips 60 are configured to pipette waste liquid after reaction in the reaction tube 200, and the reagent pipette tips 50 are configured to add reagent to the reaction tubes 200. Specifically, after the extraction reaction in the reaction tube 200 is finished, the horizontal transport mechanism 41 is driven to horizontally transport the lifting mechanism 42, the pipette tips 60 and the reagent pipette tips 50 to the position right above the reaction tube 200, then the pipette tips 60 and the reagent pipette tips 50 are transported to the reaction tubes 200 one by the lifting mechanism 42, after the pipette tips 60 completely suck the waste liquid in the reaction tube 200 and the reagent pipette tips 50 add new reagent into the reaction tube 200, the pipette tips 60 and the reagent pipette tips 50 are withdrawn from the reaction tube 200 by the lifting mechanism 42, then the waste liquid in the reaction tubes 200 is completely sucked and added with liquid in sequence according to the above method, and finally the pipette tips 60 and the reagent pipette tips 50 are transported to the initial position by the lifting mechanism 42 and the horizontal transport mechanism 41.

Referring to fig. 4, the base frame 70 has a mounting plate 71 at an upper portion thereof, the mounting plate 71 is vertically disposed and extends in a left-right direction, two guide rails 415 are disposed on the mounting plate 71 at intervals in the vertical direction, the horizontal conveying mechanism 41 is mounted on the mounting plate 71, and the horizontal conveying mechanism 41 horizontally conveys along the guide rails 415.

In the present embodiment, the horizontal conveying mechanism 41 and the lifting mechanism 42 are both belt conveying mechanisms. Next, the horizontal transfer mechanism 41 is described as an example, and the horizontal transfer mechanism 41 includes a first drive motor 411, a drive pulley 412, a conveyor belt 413, a driven pulley 414, and a guide rail 415. First driving motor 411 is installed in the rear side of mounting panel 71, action wheel 412 is installed in the front side of mounting panel 71 and is connected with first driving motor 411's motor shaft, follow driving wheel 414 install on mounting panel 71 and with action wheel 412 along left and right direction interval setting, drive wheel 412 and follow driving wheel 414 are located to the conveyer belt 413 cover respectively, elevating system 42 installs on horizontal feed mechanism 41 through a mounting bracket 43, specifically, mounting bracket 43 installs on conveyer belt 413 and moves about along conveyer belt 413, and mounting bracket 43 and guide rail 415 cooperation, lead and the gravity supports mounting bracket 43 through guide rail 415. It is understood that in other embodiments of the present application, the horizontal conveying mechanism 41 and the lifting mechanism 42 may also adopt other linear conveying mechanisms for conveying, such as a ball screw mechanism, which is not limited herein.

Referring to fig. 5 and 6, in the present embodiment, the liquid adding and extracting arm 30 includes a connecting plate 31 and a mounting seat 32, the connecting plate 31 is mounted at the output end of the lifting mechanism 42 and extends along the vertical direction, the mounting seat 32 is mounted at the bottom end of the connecting plate 31, the mounting seat 32 is flat and extends along the horizontal direction, a row of first mounting holes 321 and a row of second mounting holes 322 are formed in the mounting seat 32, the first mounting holes 321 and the second mounting holes 322 are arranged in parallel, the first mounting holes 321 and the second mounting holes 322 both penetrate through the mounting seat 32 along the vertical direction, the first mounting holes 321 are used for mounting the reagent dripping tubes 50, and the second mounting holes 322 are used for mounting the pipette tips 60. The liquid adding arm 30 of the embodiment is provided with the connecting plate 31, so that the installation seat 32 and the lifting mechanism 42 cannot interfere in the vertical direction, and meanwhile, the reagent dropper 50 and the sucker 60 are both installed on the same installation seat 32 through the installation seat 32, so that the liquid adding arm 30 can transfer once to complete two processes of liquid extracting and liquid adding simultaneously, and the liquid adding arm is simple in structure, convenient to drive and low in cost.

Referring to fig. 5 and 6, in the present embodiment, the connecting head 323 is installed in the second installation hole 322, the push plate 33 is installed below the installation seat 32, a third installation hole 331 is opened on the push plate 33 corresponding to the second installation hole 322, the bottom end of the connecting head 323 passes through the third installation hole 331 and penetrates out from the bottom surface of the push plate 33, the suction head 60 is sleeved on the connecting head 323 at a position below the push plate 33, and the push plate 33 can move downward to push the suction head 60 downward from the connecting head 323. In the initial state, the suction head 60 is sleeved at the bottom end of the connecting head 323, and the push sheet 33 and the mounting seat 32 are overlapped; when the suction head 60 is completely sucked up with waste liquid and the suction head 60 returns to the initial position, the push plate 33 will move downward and push the suction head 60 to drop the suction head 60 from the connecting head 323, and then the suction head 60 is replaced with a new one.

Specifically, the connecting plate 31 is provided with a second driving motor 34, the second driving motor 34 is connected with a screw rod 35, the screw rod 35 is vertically arranged, the screw rod 35 penetrates through the mounting seat 32 and is connected with the push sheet 33, and the screw rod 35 can be driven to rotate through the second driving motor 34 so as to drive the push sheet 33 to move up and down.

The utility model also provides a nucleic acid extraction element, including the above-mentioned drawing liquid system that adds. The nucleic acid extraction device of the cartridge of the present embodiment improves the nucleic acid extraction efficiency of the nucleic acid extraction device and reduces the cost by providing the pumping system.

The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.

Claims (10)

1. The liquid adding and extracting system is characterized by comprising a reagent conveying device, a liquid extracting control device, a liquid adding and extracting arm and a liquid adding and extracting arm transfer device, wherein the liquid adding and extracting arm transfer device is used for driving the liquid adding and extracting arm to move along the horizontal direction and the vertical direction; each reagent dropper is respectively communicated with the reagent conveying device, and the reagent conveying device is used for conveying an extraction reagent to each reagent dropper, wherein the extraction reagent is a lysis solution, a binding solution, a rinsing solution and an eluent; and each suction head is respectively communicated with the liquid pumping control device, and the liquid pumping control device is used for controlling each suction head to pump waste liquid.

2. The fluid adding and pumping system as claimed in claim 1, wherein the reagent delivering device comprises a plurality of reagent bottles and a fluid path delivering assembly, each reagent bottle stores a different extraction reagent, an input port of the fluid path delivering assembly is connected with each reagent bottle in a one-to-one correspondence manner, an output port of the fluid path delivering assembly is connected with each reagent dropper through a multi-way pipe, the fluid path delivering assembly is used for branching and delivering the reagent in each reagent bottle to each reagent dropper in a preset manner, and the fluid adding and pumping arm transferring device is used for driving each reagent dropper to move so as to drop each reagent into the reaction tube.

3. The fluid pumping system as claimed in claim 2, wherein the fluid path conveying assembly comprises a first fluid path plate and a plurality of second fluid path plates, the second fluid path plates are sequentially stacked along a first direction, the first fluid path plate is respectively attached to the second fluid path plates along a second direction, and the first direction is perpendicular to the second direction; each input port is arranged on the first liquid circuit board and distributed along the first direction, each output port is distributed along the second direction, each second liquid circuit board is provided with two output ports, each input port can be respectively communicated with each output port, and the output ports are mutually communicated in each second liquid circuit board.

4. The fluid pumping system as claimed in claim 3, wherein the first fluid passage plate has a plurality of fluid inlets and a plurality of first connecting holes, the second liquid circuit board is provided with a plurality of second connecting holes and a plurality of third connecting holes, the input port is arranged at one end of the liquid inlet hole, the other end of the liquid inlet hole is connected with one end of the first connecting hole through a first injection pump, the other end of the first connecting hole and one end of the second connecting hole are connected with a first liquid channel, the first liquid channel extends along a second direction, the other end of the second connecting hole and one end of the third connecting hole are connected with a second injection pump, the other end of the third connecting hole is connected with the output port, a normally closed first electromagnetic valve is arranged on each first liquid channel, the second connecting holes are communicated in sequence, and the third connecting holes are communicated in sequence.

5. The fluid pumping system as claimed in any one of claims 1 to 4, wherein the pumping control means comprises air pipes respectively communicating with the suction heads, and a pumping motor respectively connected to the air pipes for controlling the suction heads to generate a negative pressure to pump waste fluid, and a second solenoid valve is provided in each air pipe.

6. The fluid pumping system as defined in any one of claims 1 to 4, wherein the pumping arm transfer device comprises a horizontal conveying mechanism and a lifting mechanism slidably disposed on the horizontal conveying mechanism, and the pumping arm is mounted at an output end of the lifting mechanism.

7. The fluid pumping system as defined in claim 6, wherein the horizontal conveying mechanism and the lifting mechanism are belt conveying mechanisms.

8. The fluid pumping system as claimed in claim 6, wherein the fluid pumping arm comprises a connecting plate mounted on the lifting mechanism and extending in a vertical direction, and a mounting seat mounted at a bottom end of the connecting plate, wherein the mounting seat is provided with a row of first mounting holes and a row of second mounting holes, the first mounting holes are arranged in parallel with the second mounting holes, the first mounting holes are used for mounting the reagent dropper, and the second mounting holes are used for mounting the suction head.

9. The fluid pumping system as defined in claim 8, wherein a connection head is installed in the second installation hole, a push plate is installed below the installation seat, a bottom end of the connection head penetrates out of a bottom surface of the push plate, the suction head is sleeved on the connection head at a position below the push plate, and the push plate can move downwards to push the suction head down from the connection head.

10. The nucleic acid extraction apparatus, characterized by comprising the drawing system according to any one of claims 1 to 9.

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