CN211847925U - Culture medium nitrogen charging device - Google Patents
Culture medium nitrogen charging device Download PDFInfo
- Publication number
- CN211847925U CN211847925U CN201922432714.0U CN201922432714U CN211847925U CN 211847925 U CN211847925 U CN 211847925U CN 201922432714 U CN201922432714 U CN 201922432714U CN 211847925 U CN211847925 U CN 211847925U
- Authority
- CN
- China
- Prior art keywords
- nitrogen
- syringe needle
- exhaust
- culture medium
- needle head
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a culture medium nitrogen charging device, which is used for connecting a nitrogen charging device for charging nitrogen after a thioglycollate fluid culture medium is filled into a penicillin bottle, and comprises a nitrogen charging needle head, an exhaust needle head, a baffle plate and a nitrogen supply device; fill nitrogen syringe needle, exhaust syringe needle and pass a side of baffle, have two horizontal screw in the baffle, accessible bolt will fill nitrogen syringe needle, exhaust syringe needle respectively and be fixed in on the baffle side, when filling nitrogen, will fill nitrogen syringe needle and connect the confession nitrogen device, fill nitrogen syringe needle, exhaust syringe needle adjusting position, make exhaust syringe needle lower extreme be less than and fill nitrogen syringe needle lower extreme, fill nitrogen syringe needle lower extreme and be less than the plug lower surface, extract fast perpendicularly during sealed and fill nitrogen syringe needle and exhaust syringe needle, in addition the aluminium lid can be accomplished, whole nitrogen filling process is simple and fast, fills nitrogen effectually, enables the nitrogen gas layer and keeps on the upper portion of culture medium container, avoids the influence of outside air, has showing technical progress in this area.
Description
Technical Field
The utility model belongs to culture medium processing field especially relates to a nitrogen charging device of bottled liquid culture medium.
Background
The thioglycollate culture medium is rich in nutrient components, sodium thioglycollate and glucose are added as reducing agents to absorb oxygen in the culture medium, a lower oxidation-reduction potential is provided for anaerobic bacteria growth, and the thioglycollate serving as an oxidation-reduction indicator can indicate the oxygen-containing degree (light red in the presence of oxygen and colorless in the absence of oxygen) in the culture medium so as to facilitate sterile detection. The thioglycollate medium is mainly used for the sterility test of medicines or foods and can also be used for the culture of pathogenic microorganisms. Because of its comprehensive nutrient components, it can culture most of aerobe and anaerobe, and its preparation is simple, so that it can be extensively used in pharmaceutical industry, medical department and scientific research institute. However, the components of the culture medium are very easy to oxidize, and a pink oxidation layer is easy to appear on the upper layer of the culture medium liquid in the preparation process, so that the effect of the aseptic examination is influenced. At present, the common practice at home and abroad is to boil the culture medium to eliminate an oxide layer before inoculation, but the method consumes energy and labor, and nutrient components are destroyed after the culture medium is subjected to high-temperature treatment again, so that the authenticity and the accuracy of sterility inspection or pathogenic bacteria culture can be directly influenced.
Disclosure of Invention
For solving the above-mentioned problem that current product and technique exist, the utility model provides a culture medium nitrogen charging device, the structure includes: a nitrogen filling needle head, an exhaust needle head, a baffle plate and a nitrogen supply device; the nitrogen filling needle head and the exhaust needle head penetrate through one side edge of the baffle plate, two transverse screw holes are formed in the baffle plate, and the nitrogen filling needle head and the exhaust needle head can be fixed to the side edge of the baffle plate through bolts; the upper end of the nitrogen filling needle head is connected with a nitrogen supply device, and the upper end of the exhaust needle head is communicated with the air.
Preferably, the distance between the nitrogen filling needle and the lower end of the exhaust needle is 2-5 cm.
Preferably, the outer surface of the lower part of the nitrogen filling needle head is provided with length scales, so that the thickness of the rubber plug can be conveniently measured to match the procedure of filling the rubber plug into one third of the depth during nitrogen filling.
Preferably, the outer surface of the lower part of the exhaust needle head is provided with length scales.
Preferably, the nitrogen supply device comprises a nitrogen storage device and a gas valve.
The utility model discloses when filling nitrogen, will fill nitrogen syringe needle and connect the confession nitrogen device, hug closely plug upper surface the baffle lower surface side is hugged closely fill nitrogen syringe needle, exhaust syringe needle, plug and fill in the third degree of depth, will fill nitrogen syringe needle, exhaust syringe needle adjusting position, make and fill nitrogen syringe needle lower extreme and be less than the plug lower surface a little, exhaust syringe needle lower extreme is less than and fills nitrogen syringe needle lower extreme and be a little higher than the culture medium liquid level, during sealed, extracts rapidly perpendicularly and fills nitrogen syringe needle and exhaust syringe needle, in addition the aluminium lid can be accomplished, and whole nitrogen filling process is simple and efficient, and it is effectual to fill nitrogen, enables the nitrogen gas layer and keeps on the upper portion of culture medium container, avoids the influence of outside air, has showing technical progress in this area.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Detailed Description
Example 1
The culture medium nitrogen charging device of the utility model has the structure that: a nitrogen filling needle head 1, an exhaust needle head 2, a baffle plate 3 and a nitrogen supply device; the nitrogen filling needle head 1 and the exhaust needle head 2 penetrate through one side edge of the baffle plate 3, two transverse screw holes are formed in the baffle plate 3, and the nitrogen filling needle head 1 and the exhaust needle head 2 can be respectively fixed on the side edge of the baffle plate 3 through bolts 4; the upper end of the nitrogen filling needle head 1 is connected with a nitrogen supply device, and the upper end of the exhaust needle head 2 is communicated with the air.
More specifically, the distance between the nitrogen filling needle 1 and the lower end of the exhaust needle 2 is 2-5 cm.
More specifically, the outer surface of the lower part of the nitrogen filling needle head 1 is provided with length scales.
More specifically, the outer surface of the lower part of the exhaust needle head 2 is provided with length scales.
More specifically, the nitrogen supply device comprises a nitrogen storage device 5 and a gas valve 6.
More specifically, the baffle is made of polyurethane material, and the bolt is a plastic bolt.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A culture medium nitrogen charging device is characterized in that: the structure includes: a nitrogen filling needle head, an exhaust needle head, a baffle plate and a nitrogen supply device; the nitrogen filling needle head and the exhaust needle head penetrate through one side edge of the baffle plate, two transverse screw holes are formed in the baffle plate, and the nitrogen filling needle head and the exhaust needle head can be fixed to the side edge of the baffle plate through bolts; the upper end of the nitrogen filling needle head is connected with a nitrogen supply device, and the upper end of the exhaust needle head is communicated with the air.
2. The culture medium nitrogen-charging device according to claim 1, characterized in that: the distance between the nitrogen filling needle and the lower end of the exhaust needle is 2-5 cm.
3. The culture medium nitrogen-charging device according to claim 2, characterized in that: the outer surface of the lower part of the nitrogen filling needle head is provided with length scales.
4. The culture medium nitrogen-charging device according to claim 3, characterized in that: the outer surface of the lower part of the exhaust needle head is provided with length scales.
5. The culture medium nitrogen-charging device according to claim 1, characterized in that: the nitrogen supply device comprises a nitrogen storage device and a gas valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922432714.0U CN211847925U (en) | 2019-12-30 | 2019-12-30 | Culture medium nitrogen charging device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922432714.0U CN211847925U (en) | 2019-12-30 | 2019-12-30 | Culture medium nitrogen charging device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211847925U true CN211847925U (en) | 2020-11-03 |
Family
ID=73208350
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922432714.0U Active CN211847925U (en) | 2019-12-30 | 2019-12-30 | Culture medium nitrogen charging device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211847925U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111088150A (en) * | 2019-12-30 | 2020-05-01 | 江苏益玛生物科技有限公司 | Culture medium nitrogen filling method |
-
2019
- 2019-12-30 CN CN201922432714.0U patent/CN211847925U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111088150A (en) * | 2019-12-30 | 2020-05-01 | 江苏益玛生物科技有限公司 | Culture medium nitrogen filling method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101348826B (en) | Detecting method for aerogenic bacterium in soy sauce | |
| CN211847925U (en) | Culture medium nitrogen charging device | |
| CN211853522U (en) | Culture medium nitrogen filling needle | |
| CN106635766A (en) | Disposable membrane sterile microbiological culture dish | |
| CN106544265A (en) | A kind of device of quick detection microorganism | |
| WO2021135539A1 (en) | Fermented beverage and preparation method therefor | |
| CN111088150A (en) | Culture medium nitrogen filling method | |
| CN106434838B (en) | A kind of beer spoilage bacteria selective medium and its application | |
| CN207072950U (en) | A kind of continuous Biofermentation system based on intelligent control technology | |
| CN205443285U (en) | Body fluid blake bottle | |
| CN205710716U (en) | Ferment at the beginning of a kind of Microbiological detection of foods and increase bacterium test kit | |
| CN109022536A (en) | The method of aerogenic bacteria in bromocresol purple and Du Shi tubule combined highly effective detection vinegar | |
| CN204779581U (en) | Aseptic detection adapter of cell culture | |
| CN202379987U (en) | Heat-resisting coliform test bottle | |
| CN205152210U (en) | Sulphur glycollate culture medium nitrogen charging arrangement | |
| CN201648404U (en) | Novel two-phase culture medium of bacillus tuberculosis typus bovinus | |
| CN206862907U (en) | A kind of Microbiological detection of foods device | |
| CN204169940U (en) | The convenient vasculum of clinical laboratory | |
| CN207793267U (en) | A kind of blood culture detection report male device | |
| CN207107268U (en) | Blood gas analysis/blood specimen transmission cylinder | |
| CN207193279U (en) | A kind of culture transferring bottle | |
| CN201574151U (en) | Novel blood sample biphasic culture bottle | |
| CN110607243A (en) | Culture medium for separating fungi and yeast-like fungi and preparation method thereof | |
| CN202379985U (en) | Bacteria culture flask for multiple sampling | |
| CN217765372U (en) | Support for penicillin bottle plugging and capping sealing integrity test |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |