CN211814490U - Liquid changing culture box and automatic liquid changing device - Google Patents
Liquid changing culture box and automatic liquid changing device Download PDFInfo
- Publication number
- CN211814490U CN211814490U CN201922231200.9U CN201922231200U CN211814490U CN 211814490 U CN211814490 U CN 211814490U CN 201922231200 U CN201922231200 U CN 201922231200U CN 211814490 U CN211814490 U CN 211814490U
- Authority
- CN
- China
- Prior art keywords
- culture
- culture tank
- liquid
- changing
- base
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of medical appliances, and provides a liquid-changing culture box and an automatic liquid-changing device, wherein the liquid-changing culture box comprises a culture tank base and a culture tank cover covering the culture tank base, a growth cavity for accommodating nerves to be grown is formed between the culture tank base and the culture tank cover, and a gas exchange channel communicated with the growth cavity is formed between the two side ends of the culture tank base and the culture tank cover; the utility model provides a trade liquid culture box and trade liquid device automatically has following advantage: through making culture tank base and culture tank lid components of a whole that can function independently to adopt the mode that the lid closed to set up the culture tank lid on the culture tank base, convenient to use person opens the culture tank lid, will wait to grow the nerve and arrange the growth chamber in, and this growth chamber intercommunication has gas exchange channel, makes this growth chamber communicate in the outside, more accords with the demand of growing.
Description
Technical Field
The utility model relates to the technical field of medical appliances, more specifically say, relate to a trade liquid culture box and automatic liquid device that trades.
Background
Peripheral nerve injury repair is a common concern in multiple disciplines and research fields, such as tissue engineering, rehabilitation engineering, neurosurgery, and the like. At present, tissue engineering scaffolds, blood vessels or other nerve bundles are implanted into defective nerves to perform nerve repair mainly by methods such as tissue engineering, autografting and the like. However, autografting is limited by donors, and most tissue engineering scaffolds create an environment suitable for nerve regeneration, and defects which can be repaired by means of damaged nerve regeneration are limited. Therefore, in vitro culture and acquisition of nerve bundles by electrical stimulation, mechanical stimulation, chemical induction and the like become hot spots traced by everyone. At present, there are a lot of documents reporting from multiple levels of results, mechanisms, technical methods, etc., that electrical stimulation, mechanical stimulation and chemical intervention have effects on promoting nerve regeneration and neurite growth in vitro.
The method of engineering, chemistry and the like is adopted to intervene the growth and differentiation of nerve cells, bone cells and skin cells, and is the development direction of a plurality of fields such as the field of tissue engineering, the field of nerve engineering and the like. The utility model mainly aims at the long-term growth of mechanical intervention nerve tissues, and can also be expanded to be used for the long-term growth and differentiation of engineering means such as mechanics, electricity, chemistry and the like to intervene other kinds of cells.
The liquid changing culture tank for culturing the nerves in the prior art can not conveniently change the nerves to be grown, and has the defects of complex structure and complex operation.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a trade liquid and cultivate box to solve the complicated and complex operation's of structure technical problem who exists among the prior art.
In order to achieve the above object, the utility model discloses a technical scheme be a trade liquid culture box, including culture tank base and lid in culture tank lid on the culture tank base, the culture tank base with it is used for accommodating the growth chamber of treating the neural growth to form between the culture tank lid, the culture tank base with form between the culture tank lid communicate outside with the gas exchange passageway in growth chamber.
In one embodiment, the culture tank base comprises two oppositely arranged base bodies and two oppositely arranged bridge bodies connected between the two base bodies, and the distance between the two base bodies and the distance between the two bridge bodies are matched with the culture tank cover.
In one embodiment, a side surface of the base body close to the bridge is provided with a limit platform, the culture tank cover abuts against the limit platform when covering the culture tank base, and the limit platform is used for enabling the culture tank cover and the culture tank base to be separated by a preset distance to form the gas exchange channel.
In one embodiment, the cross section of the bridge body is in an L shape, the abutting surface of the limit table abutting against the culture tank cover is higher than the top surface of the bridge body, the bent and extended gas exchange channel is formed between the culture tank cover and the bridge body, and one end of the gas exchange channel, which is communicated with the growth cavity, is higher than one end of the gas exchange channel, which is communicated with the outside.
In one embodiment, the bridge is provided with a plurality of vent holes along the length direction thereof, and the vent holes are opposite to the two side ends of the culture tank cover.
In one embodiment, two of the seat bodies and two of the bridge bodies enclose a culture opening for disposing a culture membrane.
In one embodiment, the culture tank base is provided with a first annular step around the culture opening and a second annular step arranged between the first annular step and the culture opening, the height of the step surface of the second annular step is higher than that of the step surface of the first annular step, and the step surface of the second annular step is provided with a liquid discharge hole.
In one embodiment, a liquid discharge port is arranged on the base of the culture tank far away from the liquid discharge hole, a liquid discharge pipeline is communicated between the liquid discharge hole and the liquid discharge port, the liquid discharge pipeline is in a wave shape, and the height of the liquid discharge port is lower than that of the liquid discharge hole.
In one embodiment, the cover of the culture tank is provided with a viewing port communicated with the growth cavity.
Another object of the utility model is to provide an automatic trade liquid device, include as above trade liquid culture box.
The utility model provides an automatic trade liquid device's beneficial effect lies in:
through making culture tank base and culture tank lid components of a whole that can function independently to adopt the mode that the lid closed to set up the culture tank lid on the culture tank base, convenient to use person opens the culture tank lid, will wait to grow the nerve and arrange the growth chamber in, and this growth chamber intercommunication has gas exchange channel, makes this growth chamber communicate in the outside, more accords with the demand of growing.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a perspective view of a liquid-changing culture box according to an embodiment of the present invention;
FIG. 2 is a cross-sectional view of a liquid-changing culture box according to an embodiment of the present invention;
FIG. 3 is an exploded view of a perspective of a liquid change incubator according to an embodiment of the present invention;
FIG. 4 is an exploded view of another perspective of the liquid change incubator according to the embodiment of the present invention;
FIG. 5 is an enlarged view at "A" of FIG. 4;
FIG. 6 is another sectional view of the liquid-changing culture box according to the embodiment of the present invention.
The figures are numbered:
a culture tank base-1; a seat body-11; a bridge-12; a limiting table-13; -14, a vent hole; culture hatch-15; a first annular step-16; a second annular step-17; a liquid discharge hole-18; a liquid discharge interface-19;
a culture tank cover-2; a viewing port-21;
a gas exchange channel 3.
Detailed Description
In order to make the technical problem, technical solution and advantageous effects to be solved by the present invention more clearly understood, the following description is given in conjunction with the accompanying drawings and embodiments to illustrate the present invention in further detail. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected or indirectly connected to the other element.
It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in an orientation or positional relationship indicated in the drawings for convenience in describing the invention, and are not intended to indicate that a device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the invention.
Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating relative importance or as indicating a number of technical features. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise. The following describes the specific implementation of the present invention in more detail with reference to specific embodiments:
as shown in FIG. 1 and FIG. 2, the embodiment of the present invention provides a liquid-changing culture box, which comprises a culture tank base 1 and a culture tank cover 2 covering the culture tank base 1, wherein a growth chamber for accommodating the nerve to be grown is formed between the culture tank base 1 and the culture tank cover 2, and a gas exchange channel 3 communicating the growth chamber is formed between the two side ends of the culture tank base 1 and the culture tank cover 2.
The working principle of the liquid-changing culture box of the embodiment is as follows:
opening the culture tank cover 2, placing the nerve to be grown into the culture tank base 1, and covering the culture tank cover 2 on the culture tank base 1, so that a growth cavity is formed between the culture tank cover 2 and the culture tank base 1, and the nerve can be stimulated by various stimuli in the growth cavity to grow; meanwhile, the growth cavity is communicated with the gas exchange channel 3, and the gas exchange channel 3 is communicated with the outside, so that the nerve placed in the growth cavity can be kept in a communicated state with the outside gas.
The automatic liquid changing device provided by the embodiment has the beneficial effects that:
through making culture tank base 1 and the components of a whole that can function independently of culture tank cover 2 to adopt the mode that the lid closed to set up culture tank cover 2 on culture tank base 1, convenient to use person opens culture tank cover 2, will wait to grow the nerve and arrange growth chamber in, and this growth chamber intercommunication has gas exchange channel 3, makes this growth chamber communicate in the outside, more accords with the demand of growing.
In one embodiment, the culture tank base 1 comprises two oppositely disposed housing bodies 11 and two oppositely disposed bridge bodies 12 connecting between the two housing bodies 11, the distance between the two housing bodies 11 and the distance between the two bridge bodies 12 being matched with the culture tank cover 2. Specifically, the distance between the two seat bodies 11 is equal to the length of the culture tank cover 2, and the distance between the two bridge bodies 12 is smaller than or equal to the width of the culture tank cover 2, so that the culture tank cover 2 can be exactly fitted over the culture tank base 1.
Referring to fig. 2 to 3, in one embodiment, a position-limiting platform 13 is disposed on a side surface of the base 11 close to the bridge 12, the cover 2 abuts against the position-limiting platform 13 when covering the base 1, and the position-limiting platform 13 is used to space a predetermined distance between the cover 2 and the base 1 to form the gas exchange channel 3. Specifically, the position restricting stage 13 is used to space the culture tank cover 2 and the bridge 12 a predetermined distance apart to form the gas exchange channel 3. It can be understood that the design of the limiting table 13 enables the culture tank cover 2 to form the gas exchange channel 3 when covering the culture tank base 1, and the gas exchange channel 3 does not need to be formed by other complex structures, so the liquid change culture box with the gas exchange channel 3 in the embodiment has a simple structure.
In one embodiment, the cross section of the limiting platform 13 is in a shape of Chinese character 'pin', the cross section of the bridging body 12 is in a shape of Chinese character 'L', the limiting platform 13 abuts against the culture tank cover 2, the abutting surface of the limiting platform is higher than the top surface of the bridging body 12, a bent and extended gas exchange channel 3 is formed between the culture tank cover 2 and the bridging body 12, the gas exchange channel 3 is in a shape of Chinese character 'Z', and one end of the gas exchange channel 3, which is communicated with a growth cavity, is higher than one end of the gas exchange channel 3, which is communicated with the outside. It can be understood that, through the gas exchange channel 3 with the set shape, when external air enters the growth cavity, the growth cavity can be kept in a state of being communicated with the external air through the bent gas exchange channel 3, and external air is prevented from directly flowing into the growth cavity to cause bacteria contamination.
In one embodiment, the bridge member 12 is provided with a plurality of vent holes 14 along its length, and the vent holes 14 are formed at opposite ends of the cover 2. Specifically, the vent hole 14 is communicated with the growth cavity, and the vent hole 14 is communicated with the gas exchange channel 3, so that the growth cavity can be communicated with external gas as much as possible by arranging a plurality of vent holes 14, and gas circulation is smoother.
Referring to fig. 4 to 6, in an embodiment, the two seat bodies 11 and the two bridge bodies 12 enclose a culture opening 15, and the culture opening 15 is used for disposing a culture membrane. It is understood that the culture opening 15 is closed by attaching a culture film having good biocompatibility, such as an Aclar film (barrier film), to the culture opening 15, and tissue culture is performed on the culture film.
In one embodiment, the culture tank base 1 is provided with a first annular step 16 around the culture opening 15 and a second annular step 17 provided between the first annular step 16 and the culture opening 15, the height of the step surface of the second annular step 17 is higher than that of the first annular step 16, and the liquid discharge hole 18 is provided on the step surface of the second annular step 17. It can be understood that the first annular step 16 is provided to facilitate the attachment of the culture membrane to the culture tank base 1 while avoiding the bottom from being unstable after the attachment of the membrane. In addition, the second annular step 17 is higher than the first annular step 16 by 0.5mm, so the distance between the liquid discharge hole 18 arranged on the second annular step 17 and the culture film attached on the second annular step 17 is 0.5mm, the possibility of blocking the liquid discharge hole 18 when the culture film is attached is avoided, meanwhile, the distance between the liquid discharge hole 18 and the culture film is only 0.5mm, the height of the liquid discharge hole 18 is reasonable when waste liquid is extracted, and all waste liquid in the gap of the culture film can be extracted by utilizing liquid tension.
In one embodiment, a liquid discharge port 19 is provided on the culture tank base 1 at a position away from the liquid discharge hole 18, a liquid discharge pipe is provided in communication between the liquid discharge hole 18 and the liquid discharge port 19, the liquid discharge pipe is in a wave shape, and the height of the liquid discharge port 19 is lower than that of the liquid discharge hole 18. It will be appreciated that the return of waste liquid or the ingress of external bacteria during movement is prevented by the provision of a corrugated drain.
In one embodiment, cover 2 is provided with a viewing port 21 communicating with the growth chamber. It will be appreciated that the growth of the nerve in the growth chamber can be directly observed through the viewing port 21, and preferably a transparent film is provided at the viewing port 21.
Another object of the utility model is to provide an automatic trade liquid device, include as above trade liquid culture box.
The automatic liquid changing device is used for changing a culture medium for the environment for growing nerves in the process of extracting long-section nerves.
In the existing nerve treatment technology, "autografting" is regarded as the "gold standard" of clinical treatment of peripheral nerve injury. However, "autografting" is often limited to donor sources, and most currently involve the use of nerve bundles in other, non-fit critical functional areas of the patient as donors, and sacrifice of other nerve functions in the patient. Another approach is to use body tubular tissue such as blood vessels, trachea, etc. for "bridging" to create an environment suitable for nerve growth, but is limited to the donor as in the above approach. With the development of tissue engineering technology, various types of tissue engineering scaffolds are created, and the surface of the tissue engineering scaffold is modified by designing a proper tissue engineering scaffold to induce nerve regeneration. Some institutional workers have had more attempts and efforts in this regard to using chitosan as a scaffolding material and have demonstrated that this approach does not treat defects exceeding 30 mm. In view of the above problems, a method for obtaining long nerve segments in vitro has been proposed: the axon bundle emitted from the dorsal root ganglion is pulled in one direction by external force to obtain long-section active nerve bundles, and then the long-section active nerve bundles are grafted and transplanted by combining a tissue engineering method. Through experimental verification, nerve can be elongated through the mode that the tractive was cultivateed, but owing to need cultivate for a long time in the experimentation, through a period of cultivation back, because the accumulation of the consumption of nutrition and metabolic waste needs to be changed the culture medium, and manual liquid changing is not only loaded down with trivial details and has the fungus risk of dying, consequently, the utility model discloses an automatic liquid changing device has mainly been designed to this kind of condition.
The utility model discloses mainly be applied to and acquire long-range nerve bundle, but also can be used to the long-term culture of other biological tissue simultaneously.
The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. The utility model provides a trade liquid and cultivate box, its characterized in that, including the culture trough base with cover in culture trough cover on the culture trough base, the culture trough base with it is used for acceping the growth chamber of treating the neural growth to form between the culture trough cover, the culture trough base with it communicates the outside with to form between the culture trough cover the gas exchange passageway in growth chamber.
2. The broth changing culture cassette according to claim 1, wherein the culture tank base comprises two oppositely disposed housing bodies and two oppositely disposed bridge bodies connected between the two housing bodies, and a distance between the two housing bodies and a distance between the two bridge bodies are fitted to the culture tank cover.
3. The liquid-changing culture box according to claim 2, wherein a position-limiting platform is provided on a side of the base body close to the bridge member, the culture tank cover abuts against the position-limiting platform when covering the culture tank base, and the position-limiting platform is used for spacing a predetermined distance between the culture tank cover and the culture tank base to form the gas exchange channel.
4. The liquid-changing culture box according to claim 3, wherein the cross section of the bridge body is in an L shape, the abutting surface of the limiting table abutting on the culture tank cover is higher than the top surface of the bridge body, the gas exchange channel extending in a bending manner is formed between the culture tank cover and the bridge body, and one end of the gas exchange channel, which is communicated with the growth cavity, is higher than one end of the gas exchange channel, which is communicated with the outside.
5. The liquid-changing culture box according to claim 2, wherein the bridge member is provided with a plurality of vent holes along its own length, and the vent holes are opposed to both side ends of the culture tank cover.
6. The liquid-changing culture box according to claim 2, wherein the two seat bodies and the two bridge bodies enclose a culture opening for arranging a culture membrane.
7. The liquid-changing culture box according to claim 6, wherein the culture tank base is provided with a first annular step around the culture opening and a second annular step provided between the first annular step and the culture opening, the height of the step surface of the second annular step is higher than that of the step surface of the first annular step, and a liquid discharge hole is provided in the step surface of the second annular step.
8. The liquid-changing culture box according to claim 7, wherein a liquid discharge port is provided on the bottom of the culture tank at a position away from the liquid discharge hole, a liquid discharge pipe is provided in communication between the liquid discharge hole and the liquid discharge port, the liquid discharge pipe is in a wave shape, and the height of the liquid discharge port is lower than the height of the liquid discharge hole.
9. The broth changing culture cassette according to any one of claims 1 to 8, wherein the culture tank cover is provided with a viewing port communicating with the growth chamber.
10. An automatic liquid-changing apparatus comprising the liquid-changing culture cassette according to any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922231200.9U CN211814490U (en) | 2019-12-12 | 2019-12-12 | Liquid changing culture box and automatic liquid changing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922231200.9U CN211814490U (en) | 2019-12-12 | 2019-12-12 | Liquid changing culture box and automatic liquid changing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211814490U true CN211814490U (en) | 2020-10-30 |
Family
ID=73140975
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922231200.9U Active CN211814490U (en) | 2019-12-12 | 2019-12-12 | Liquid changing culture box and automatic liquid changing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211814490U (en) |
-
2019
- 2019-12-12 CN CN201922231200.9U patent/CN211814490U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104726332B (en) | One kind cerebral tissue external printing culture systems and method | |
| CA2346191A1 (en) | Process for producing a vascularized bioartificial tissue and an experimental reactor for carrying out the process | |
| WO2009118140A2 (en) | Perfusable bioreactor for the production of human or animal tissues | |
| CN106635961A (en) | Cell culture medium for preparing human skin flbroblast sheet and using method of cell culture medium | |
| CN208883905U (en) | The adjustable cell culture apparatus of medical experiment oxygen-supplying amount | |
| WO2011105818A2 (en) | Cell culture unit and cell culture device including the same | |
| CN104178422A (en) | Nerve axon stretching grower | |
| CN211814490U (en) | Liquid changing culture box and automatic liquid changing device | |
| US6623956B1 (en) | Embryo-culturing incubator assembly | |
| CN207294809U (en) | A kind of neural stem cell culture flask | |
| EP2800807B1 (en) | Bioreactor composed of watertight chamber and internal matrix for the generation of cellularized medical implants | |
| CN207987246U (en) | A kind of biological perfusion reaction system applied to bone tissue engineer micro-assembly robot culture | |
| CN201587946U (en) | In vitro co-culture apparatus for multiple types of cells | |
| CN218388966U (en) | Batch cultivation box for traditional Chinese medicine seedlings | |
| CN110951612A (en) | Automatic liquid changing device | |
| CN211645181U (en) | Culture medium storage box and automatic liquid changing device | |
| CN109757312A (en) | Cordyceps militaris cultivates equipment | |
| CN215935771U (en) | Wild glossy ganoderma is with multi-functional artificial containers | |
| CN101676382B (en) | Biomimetic tissue culture room | |
| CN204968716U (en) | Wheat breeding is with cultivating basin | |
| CN213977678U (en) | Constant temperature and humidity incubator | |
| CN211035948U (en) | Modular three-dimensional perfusion cell culture system | |
| CN105368712B (en) | A kind of organization engineering skin industrialization culture apparatus | |
| CN204058481U (en) | A kind of neural axon tractive growing apparatus | |
| CN209722173U (en) | A kind of skin keratin formation cell gas-liquid face culture systems |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |