CN211718609U - Objective lens combination and full-glass digital acquisition system - Google Patents
Objective lens combination and full-glass digital acquisition system Download PDFInfo
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- CN211718609U CN211718609U CN202020097882.9U CN202020097882U CN211718609U CN 211718609 U CN211718609 U CN 211718609U CN 202020097882 U CN202020097882 U CN 202020097882U CN 211718609 U CN211718609 U CN 211718609U
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Abstract
The application discloses objective combination and full slide digital acquisition system, this objective combination includes: the device comprises a piezoelectric ceramic mounting plate, an objective lens mounting frame, a transverse and vertical displacement conversion mechanism, a piezoelectric ceramic combination, a first objective lens and a second objective lens; the transverse and vertical displacement conversion mechanism is formed on the piezoelectric ceramic mounting plate, the objective lens mounting frame is horizontally mounted on the transverse and vertical displacement conversion mechanism, the piezoelectric ceramic combination is mounted in the transverse and vertical displacement conversion mechanism, and the first objective lens and the second objective lens are mounted on the objective lens mounting frame in parallel; the piezoelectric ceramic combination comprises a piezoelectric ceramic device, and the transverse-vertical displacement conversion mechanism converts the size change of the piezoelectric ceramic device in the X-axis direction into the vertical movement of the objective lens mounting frame in the Z-axis direction, so that the first objective lens and the second objective lens are driven to displace up and down, and the fine adjustment of the focal length is realized. By adopting the method and the device, the image definition in the slide scanning process can be greatly improved.
Description
Technical Field
The application relates to the technical field of slide scanning, in particular to an objective lens combination and a full-slide digital acquisition system.
Background
In the past, doctors observed cells or tissues carried on slides through a microscope to obtain information about patients, which is often a complicated process, not only is the slides not easy to store and carry, but also the slides slowly fade over time.
At present, the combination of electromechanical automatic control and image analysis technology makes the high-definition digital acquisition and analysis of slides become a trend, and digital acquisition systems are known. However, the applicant has appreciated that existing digital acquisition systems tend to be primarily targeted at higher scanning speeds, resulting in the problem of lower image sharpness during slide scanning.
SUMMERY OF THE UTILITY MODEL
The technical problem to be solved by the present application is to provide an objective lens combination and a full-slide digital acquisition system, which can greatly improve the image definition in the slide scanning process, aiming at the above defects in the prior art.
The technical scheme adopted by the application for solving the technical problem is as follows:
according to one aspect of the present application, an objective lens assembly includes: the device comprises a piezoelectric ceramic mounting plate, an objective lens mounting frame, a transverse and vertical displacement conversion mechanism, a piezoelectric ceramic combination, a first objective lens and a second objective lens; the transverse and vertical displacement conversion mechanism is formed on the piezoelectric ceramic mounting plate, the objective lens mounting frame is horizontally mounted on the transverse and vertical displacement conversion mechanism, the piezoelectric ceramic combination is mounted in the transverse and vertical displacement conversion mechanism, and the first objective lens and the second objective lens are mounted on the objective lens mounting frame in parallel; the piezoelectric ceramic combination comprises a piezoelectric ceramic device, and the transverse-vertical displacement conversion mechanism converts the size change of the piezoelectric ceramic device in the X-axis direction into the vertical movement of the objective lens mounting frame in the Z-axis direction, so that the first objective lens and the second objective lens are driven to displace up and down, and the fine adjustment of the focal length is realized.
In some embodiments, the lateral-vertical displacement conversion mechanism comprises: a left upper arm, a right upper arm, a left lower arm, a right lower arm and a suspension part; the transverse-vertical displacement conversion mechanism is of a symmetrical structure; two symmetrical gaps are arranged on the peripheries of the left upper arm, the right upper arm, the left lower arm, the right lower arm and the suspension part; the left upper arm, the right upper arm, the left lower arm and the right lower arm are integrated to form a piezoelectric ceramic accommodating cavity for correspondingly installing the piezoelectric ceramic combination.
In some embodiments, the left upper arm, the right upper arm, the left lower arm and the right lower arm together form a vertical and horizontal displacement conversion bridge, and the vertical and horizontal displacement conversion bridge converts the dimension change of the piezoceramic device in the X-axis direction into the up-and-down motion of the combination of the left lower arm and the right lower arm in the Z-axis direction.
In some embodiments, the bottom of the hanging part is provided with a notch, and the shape of the notch is matched with the shape of the bottom of the two gaps, so that the hanging part can be displaced up and down.
In some embodiments, further comprising: the mounting adjusting bolt is used for forcing the suspension part to move upwards so as to facilitate the mounting of the piezoelectric ceramic combination; the bottom of the hanging part is provided with an adjusting bolt accommodating cavity for accommodating the mounting adjusting bolt correspondingly.
In some embodiments, the piezoelectric ceramic combination further comprises: the first insulating part, the second insulating part and the supporting part; wherein, the first insulator and the second insulator are respectively arranged at the left end and the right end of the piezoelectric ceramic device; the support member is disposed between the second insulator and the structure on the piezoelectric ceramic mounting plate.
In some embodiments, the second insulator is ceramic and is bowl-shaped, and the support is cup-shaped.
In some embodiments, the first insulator is made of plastic.
In some embodiments, further comprising: a base plate; the vertical piezoelectric ceramic mounting plate is mounted on the vertical bottom plate.
According to one aspect of the application, a full-glass digital acquisition system comprises the objective lens combination; the first objective lens is an objective lens with relatively low magnification, the second objective lens is an objective lens with relatively high magnification, and the first objective lens and the second objective lens are switched into scanning objective lenses under the action of the objective lens combined displacement mechanism so as to scan a scanning area where an object to be detected is located.
The beneficial effects of this application lie in, ingenious through reaching the installation of piezoceramics combination and the realization of horizontal and vertical displacement conversion mechanism with the piezoceramics mounting panel, both can install two objective side by side together, can realize the focus fine setting of high magnification's second objective again through controlling piezoceramics device, can improve the image definition in the slide scanning process greatly.
Drawings
The present application will be further described with reference to the accompanying drawings and examples, in which:
FIG. 1 is a schematic diagram of the structure of the whole slide digital acquisition system of the present application.
Fig. 2 is a schematic perspective view of the objective lens assembly of the present application.
Fig. 3 is a front view of the objective lens assembly of the present application.
Fig. 4 is an exploded view of the objective lens assembly of the present application.
FIG. 5 is a front view of a piezoelectric ceramic mounting plate in the objective lens assembly of the present application.
FIG. 6 is a flow chart illustrating a slide scanning process of the present application.
Wherein the reference numerals are as follows: 10 full slide digital acquisition system 1 base 2 stage 3 slide 4 stage displacement mechanism 5 objective combination displacement mechanism 6 objective combination 7 optical system 8 oil drip system 61 bottom plate 62 piezoceramic mounting plate 63 objective mounting rack 631, 632 mounting hole 633, 634 notch 64 horizontal and vertical displacement switching mechanism 641 upper left arm 642 upper right arm 643 upper left arm 644 lower right arm 645 suspension 646 piezoceramic receiving cavity 647 gap 648 notch 649 adjusting bolt receiving cavity 65 piezoceramic combination 651 piezoceramic device 652 first insulator 653 second insulator 654 support 66 mounting adjusting bolt 67 first objective 68 second objective.
Detailed Description
The preferred embodiments of the present application will now be described in detail with reference to the accompanying drawings.
Referring to fig. 1, fig. 1 is a schematic diagram of the structure of the whole slide digital acquisition system of the present application. The present application provides a full-slide digital acquisition system 10, which includes: a base 1, a stage 2, a slide 3, a stage displacement mechanism 4, an objective lens combination displacement mechanism 5, an objective lens combination 6, an optical system 7, an oil dropping system 8 and a circuit system (not shown).
The base 1 is the physical carrying mechanism of the full-slide digital acquisition system 10. For example, the base 1 takes the form of a marble base, which possesses a very low motion profile and a stability on the order of nanometers within the motion stroke.
The stage 2 is used for carrying a slide 3, and the stage displacement mechanism 4 is used for driving the stage 2 to displace on a horizontal plane formed by an X axis and a Y axis. For example, the stage displacement mechanism 4 has an X-axis control motor for driving the stage 2 to displace on the X-axis; the stage displacement mechanism 4 further comprises a Y-axis control motor for driving the stage 2 to displace on the Y-axis.
The objective lens assembly 6 is used for scanning an object to be inspected carried on the slide 3. The object to be detected may be a tissue or a cell. The objective combination 6 has two objective lenses in parallel: and the first objective lens and the second objective lens can be switched under the action of the objective lens combined displacement mechanism 4 to be used as scanning objective lenses for scanning a scanning area where an object to be detected is located. Wherein the first objective lens is an objective lens with a relatively low magnification and the second objective lens is an objective lens with a relatively high magnification. For example, for tissue as the object to be detected, an objective lens with a lower magnification may be applicable; for cells as the object to be detected, a higher magnification objective lens is used. Optionally, the first objective lens is a 20-fold objective lens, and the second objective lens is a 40-fold objective lens; alternatively, the first objective lens is a 10-fold objective lens and the second objective lens is a 100-fold objective lens. Which will be described in detail later.
The objective lens combination displacement mechanism 5 is used for driving the objective lens combination 6 to displace on a vertical plane formed by a Z axis and an X axis. For example, the objective lens combination displacement mechanism 5 has a Z-axis control motor, which is used for displacing the objective lens combination 6 on the Z-axis, that is, driving the two objective lenses to move downward close to the slide 3 or move upward away from the slide 3, so that the coarse adjustment of the focal length of the objective lenses can be realized by means of the Z-axis control motor, which can also be considered as a coarse focusing mechanism of the system; in addition, the objective lens combination displacement mechanism further has an X-axis control motor for driving the objective lens combination 6 to displace on the X-axis, that is, driving the two objective lenses to move transversely, so as to select which objective lens of the two objective lenses is currently aligned with the slide 3, in other words, the two objective lenses can be switched relative to the scanning area of the slide 3 where the object to be detected is located through transverse movement. In this embodiment, the X-axis control motor is a stepping motor and is matched with a lead screw for transmission.
The optical system 7 further processes the optical signal captured by the objective combination 6 and converts it into an electrical signal. The optical system 7 includes, for example, a digital camera.
The oil dripping system 8 is used for carrying out oil dripping treatment on the slide 3 on the object stage 2 so as to obtain a better detection effect. The oil dripping system 8 includes, for example, an oil storage container, an oil pipe connected to the oil storage container, and an electrically controlled pump disposed in a passage of the oil pipe and controlled to be opened or closed to thereby control whether to drip oil onto the upper surface of the slide 3.
The circuit system is used for controlling the work of the objective table displacement mechanism 4, the objective combination displacement mechanism 5, the objective combination 6 and the oil dripping system 8, receiving the electric signals provided by the optical system 7 and obtaining the scanning image of the object to be detected. For example, the circuitry includes: the piezoelectric motor controller comprises a piezoelectric motor driver, a piezoelectric motor controller, a communication interface, a positioning control system and a peripheral circuit, wherein the peripheral circuit further comprises a power supply circuit, an interface circuit of a grating encoder and a DSP (Digital Signal Processing) chip, a piezoelectric motor start-stop, linear motion and frequency modulation speed regulation circuit and the like.
The full-glass digital acquisition system 10 adopts a piezoelectric nano flexible hinge mechanism (namely, the combination of a piezoelectric ceramic mounting plate 62 and a piezoelectric ceramic combination 65 which will be introduced later) and a high-resolution displacement sensor (such as SGS or a capacitor) as a fine focusing mechanism of the system, and the fine focusing mechanism has ms (millisecond) level small signal response time and completely frictionless motion characteristics, so that rapid Z-axis switching during single-point acquisition is ensured; the scanning of the different magnifications (i.e. the first objective lens 67 and the second objective lens 68, which will be described later) is all controlled by the linear grating scale controller, the position during the fully automatic dynamic scanning is precisely controlled and the dynamic following focusing of the fine focusing mechanism during the scanning is ensured.
Referring to fig. 2, 3 and 4, fig. 2 is a schematic perspective view of the objective lens assembly of the present application. Fig. 3 is a front view of the objective lens assembly of the present application. Fig. 4 is an exploded view of the objective lens assembly of the present application. The present application provides an objective lens assembly 6, comprising: the device comprises a base plate 61, a piezoelectric ceramic mounting plate 62, an objective lens mounting frame 63, a transverse-vertical displacement conversion mechanism 64, a piezoelectric ceramic combination 65, a mounting adjusting bolt 66, a first objective lens 67 and a second objective lens 68. In the present embodiment, the first objective lens 67 is a 20-fold objective lens; the second objective lens 68 is a 40-fold objective lens.
A vertical piezo ceramic mounting plate 62 is mounted on the vertical base plate 61. The vertical and horizontal displacement conversion mechanisms 64 are formed on the piezoelectric ceramic mounting plate 62. The objective lens mount 63 is horizontally mounted on the vertical and horizontal displacement conversion mechanism 64. The piezoelectric ceramic combination 65 is installed in the vertical and horizontal displacement conversion mechanism 64. The first objective lens 67 and the second objective lens 68 are mounted side by side on the objective lens mount 63, with the first objective lens 67 and the second objective lens 68 facing the slide 3 along the Z-axis direction (see also fig. 1).
The objective mounting bracket 63 has two mounting holes 631 and 632 for respectively mounting the first objective 67 and the second objective 68. Notches 633 and 634 are formed on the outer sides of the mounting holes 631 and 632, and fasteners inserted through the notches 633 and 634 can mount and fix the first objective lens 67 and the second objective lens 68 in the two mounting holes 631 and 632, respectively.
The piezoelectric ceramic composition 65 includes: a piezoceramic device 651, a first insulator 652, a second insulator 653, and a support 654. The first insulator 652 and the second insulator 653 are respectively disposed at the left and right ends of the piezoelectric ceramic device 651, and function as a support and an insulation. A first insulator 652 is provided between the left end of the electroceramic device 651 and the structure on the piezoceramic mounting plate 62. A second insulator 653 is provided between the right end of the device 651 and the support 654, and the support 654 is provided between the second insulator 653 and the structure on the piezo ceramic mounting plate 62, to provide the necessary support.
For example, the first insulator 652 is a plastic material. The second insulating member 653 is made of ceramic and is bowl-shaped. The support 654 is cup-shaped. The bottom of the support 654 is bonded to the piezoceramic mounting plate 62, and the bottom of the second insulating member 653 is received in the receiving cavity of the support 654. The left and right ends of the first insulator 652 are bonded to the piezoelectric ceramic mounting plate 62 and the piezoelectric ceramic device 651, respectively.
The piezo-ceramic device 651 extends horizontally along the X-axis, and when the control voltage thereon changes, it will deform in an extending-retracting manner along the X-axis direction, and the dimensional change in the X-axis direction can be converted into the up-and-down movement of the objective lens mounting bracket 63 in the Z-axis direction through the transmission of the horizontal-vertical displacement conversion mechanism 64, so as to drive the first objective lens 67 and the second objective lens 68 to move up and down, thereby achieving the fine tuning of the objective lens focal length. The vertical and horizontal displacement conversion mechanism 64 will be described in detail below.
Referring to fig. 5, fig. 5 is a front view of a piezoelectric ceramic mounting plate in the objective lens assembly of the present application. The present application proposes a lateral-to-vertical displacement conversion mechanism 64 formed on a piezoelectric ceramic mounting plate 62, which includes: a left upper arm 641, a right upper arm 642, a left lower arm 643, a right lower arm 644, and a suspension 645. The vertical-horizontal displacement conversion mechanism 64 has a symmetrical structure.
Two symmetrical gaps 647 are provided around the periphery of the upper left arm 641, the upper right arm 642, the lower left arm 643, the lower right arm 644 and the suspension 645. One of the gaps 647 is disposed on the periphery of the left upper arm 641, the left lower arm 643 and the left half of the hanging portion 645; another gap 647 is provided around the periphery of the right upper arm 642, the right lower arm 644 and the right half of the suspension portion 645 to form a gap 647.
The left upper arm 641, the right upper arm 642, the left lower arm 643 and the right lower arm 644 are enclosed to form a piezoelectric ceramic receiving cavity 646 for correspondingly installing the piezoelectric ceramic assembly 65. It is understood that the upper left arm 641, the upper right arm 642, the lower left arm 643 and the lower right arm 644 together form a vertical-horizontal displacement conversion bridge, which can convert the dimension change of the piezoceramic device 651 in the X-axis direction into the vertical-horizontal movement of the joint (and the suspension portion 645 connected to the joint) of the lower left arm 643 and the lower right arm 644 in the Z-axis direction (for example, the vertical-horizontal movement has a dimension of 125000 nm to 140000 nm, and the deviation is derived from the processing process of each piezoceramic mounting plate 62). A suspension portion 645 is provided below the vertical and horizontal displacement conversion bridge, and the objective lens mount 63 described above is fixed to the suspension portion 645.
The bottom of the hanging portion 645 is provided with a notch 648. The shape of the notch 648 is matched with the shapes of the bottoms of the two gaps 647, so that the suspension 645 is almost completely separated from the limit of other parts on the piezoelectric ceramic mounting plate 62 (the other parts on the piezoelectric ceramic mounting plate 62 have a certain limit function by virtue of the structure between the notch 648 and the gap 647), and the up-and-down displacement of the suspension 645 is facilitated.
The bottom of the hanging portion 645 is provided with an adjustment bolt receiving cavity 649 for receiving the aforementioned mounting adjustment bolt 66.
It should be noted that, by adjusting the mounting adjustment bolt 66 (turning inward), the hanging portion 645 is forced to move upward, and further the lateral dimension of the piezoelectric ceramic receiving cavity 646 is forced to increase, so as to open the piezoelectric ceramic receiving cavity 646 and facilitate the mounting of the piezoelectric ceramic assembly 65. Once the piezo-ceramic combination 65 has been installed in place, the installation adjustment screw 66 is adjusted (screwed outward) to remove the upward displacement force it exerts on the suspension 645.
With reference to fig. 1 and 6, the slide scanning operation of the full-slide digital acquisition system 10 of the present application generally includes:
1) after the full-glass digital acquisition system 10 is started, initializing, and controlling the objective table displacement mechanism 4 and the objective lens combined displacement mechanism 5 to recover to the initial positions;
2) the object stage 2 is driven by the object stage displacement mechanism 4 to be in a first position so as to receive and place a slide 3 carrying an object to be detected;
3) positioning a scanning area of an object to be detected carried on the slide 3 by using a digital camera in the optical system 7; that is, the digital camera can know the position of the object to be detected on the slide, and at this time, the area where the position of the object to be detected is located is regarded as the scanning area where the object to be detected needs to be scanned is located. In the slide scanning process, the scanning area may be further subdivided into a plurality of sub-scanning areas, which is not limited herein.
4) The oil dripping system 8 is controlled to drip oil to the slide 3;
5) for an object to be detected, switching between the first objective lens 67 and the second objective lens 68 in the objective lens combination 6 is performed by the objective lens combination displacement mechanism 5, and one of the objective lenses is selected as a scanning objective lens to be ready for scanning; that is, for different objects to be detected, objective lenses with different magnification factors can be selected as scanning objective lenses, so that the image definition in the slide scanning process is ensured;
6) the objective table 2 is driven by the objective table displacement mechanism 4 to be at a second position to wait for the scanning objective lens to scan about the scanning area;
7) under the driving of an objective lens combination displacement mechanism 5 (a Z-axis control motor and an X-axis control motor are matched with a piezoelectric ceramic combination 65), the scanning objective lens carries out point-by-point coarse focusing and fine focusing on a scanning area where an object to be detected is located so as to form a curved surface in a fitting manner;
8) the full-glass digital acquisition system 10 scans the scanning area where the object to be detected is located according to the curved surface formed by fitting, and acquires a scanning image; the scanning mode may be S-shaped, that is, progressive scanning, and is not limited herein;
9) the stage 2 is driven by the stage displacement mechanism 4 to a first position for removal of the scanned slide 3.
To this end, the full slide digital acquisition system 10 completes a slide scanning process.
The beneficial effect of this application lies in, ingeniously through reaching the installation of piezoceramics combination 65 and the realization of horizontal and vertical displacement shifter 64 with piezoceramics mounting panel 62, both can be in the same place two objective 67, 68 of different magnification are installed side by side, can realize the fine setting of objective focus through controlling piezoceramics device 651 again to improve the image definition in the slide scanning process effectively.
In other words, the full-glass digital acquisition system 10 of the present application adopts the objective lens combination 6 (i.e. the real dual objective lens) in combination with the piezoelectric ceramic to perform the rough focusing and the fine focusing on the object to be detected, and has: high resolution, high precision, strong anti-electromagnetic interference capability, high response speed, simple and reliable control and the like.
It should be understood that the above embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the same, and those skilled in the art may modify the technical solutions described in the above embodiments or substitute some technical features thereof; and such modifications and substitutions are intended to fall within the scope of the appended claims.
Claims (10)
1. An objective lens assembly, comprising: the device comprises a piezoelectric ceramic mounting plate, an objective lens mounting frame, a transverse and vertical displacement conversion mechanism, a piezoelectric ceramic combination, a first objective lens and a second objective lens; the transverse and vertical displacement conversion mechanism is formed on the piezoelectric ceramic mounting plate, the objective lens mounting frame is horizontally mounted on the transverse and vertical displacement conversion mechanism, the piezoelectric ceramic combination is mounted in the transverse and vertical displacement conversion mechanism, and the first objective lens and the second objective lens are mounted on the objective lens mounting frame in parallel; the piezoelectric ceramic combination comprises a piezoelectric ceramic device, and the transverse-vertical displacement conversion mechanism converts the size change of the piezoelectric ceramic device in the X-axis direction into the vertical movement of the objective lens mounting frame in the Z-axis direction, so that the first objective lens and the second objective lens are driven to displace up and down, and the fine adjustment of the focal length is realized.
2. An objective lens assembly as recited in claim 1, wherein the lateral-to-vertical displacement converting mechanism comprises: a left upper arm, a right upper arm, a left lower arm, a right lower arm and a suspension part; the transverse-vertical displacement conversion mechanism is of a symmetrical structure; two symmetrical gaps are arranged on the peripheries of the left upper arm, the right upper arm, the left lower arm, the right lower arm and the suspension part; the left upper arm, the right upper arm, the left lower arm and the right lower arm are integrated to form a piezoelectric ceramic accommodating cavity for correspondingly installing the piezoelectric ceramic combination.
3. An objective lens assembly as recited in claim 2, wherein the left upper arm, the right upper arm, the left lower arm and the right lower arm together form a vertical-horizontal displacement conversion bridge for converting the dimension change of the piezoceramic device in the X-axis direction into the vertical movement of the joint of the left lower arm and the right lower arm in the Z-axis direction.
4. An objective lens assembly as recited in claim 2, wherein the suspension portion has a notch formed at a bottom thereof, the notch having a shape matching a shape of a bottom of the two gaps to facilitate the up-and-down displacement of the suspension portion.
5. An objective lens combination as recited in claim 1, further comprising: the mounting adjusting bolt is used for forcing the suspension part to move upwards so as to facilitate the mounting of the piezoelectric ceramic combination; the bottom of the hanging part is provided with an adjusting bolt accommodating cavity for accommodating the mounting adjusting bolt correspondingly.
6. An objective combination as recited in claim 1, characterized in that the piezo-ceramic combination further comprises: the first insulating part, the second insulating part and the supporting part; wherein, the first insulator and the second insulator are respectively arranged at the left end and the right end of the piezoelectric ceramic device; the support member is disposed between the second insulator and the structure on the piezoelectric ceramic mounting plate.
7. An objective lens assembly as recited in claim 6, wherein the second insulating member is ceramic and is bowl-shaped, and the support member is cup-shaped.
8. An objective lens assembly as recited in claim 6, wherein the first insulator is made of plastic.
9. An objective combination as claimed in any one of claims 1 to 8, further comprising: a base plate; the vertical piezoelectric ceramic mounting plate is mounted on the vertical bottom plate.
10. A full-slide digital acquisition system comprising the objective lens assembly of any one of claims 1 to 9; the first objective lens is an objective lens with relatively low magnification, the second objective lens is an objective lens with relatively high magnification, and the first objective lens and the second objective lens are switched into scanning objective lenses under the action of the objective lens combined displacement mechanism so as to scan a scanning area where an object to be detected is located.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111045203A (en) * | 2020-01-15 | 2020-04-21 | 深圳市生强科技有限公司 | Objective lens combination and full-glass digital acquisition system |
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