CN211678270U

CN211678270U - Leukemia lymphocyte separation and extraction device

Info

Publication number: CN211678270U
Application number: CN202020205523.0U
Authority: CN
Inventors: 朱娟; 付菊花
Original assignee: Third Xiangya Hospital of Central South University
Current assignee: Third Xiangya Hospital of Central South University
Priority date: 2020-02-25
Filing date: 2020-02-25
Publication date: 2020-10-16
Anticipated expiration: 2030-02-25

Abstract

The utility model discloses a leukemia lymphocyte separation extraction element, concretely relates to lymphocyte draws technical field, which comprises a rotary table, carousel bottom fixedly connected with pivot, the carousel bottom is equipped with the motor, motor output shaft and pivot bottom fixed connection, the carousel top is equipped with draws the mechanism, draw the mechanism and include a plurality of reagent pipes, it is a plurality of the carousel top is all run through to reagent socle end. The utility model discloses a motor work drives the pivot and rotates to drive the carousel and rotate, carry out centrifugal separation to the blood sample in the reagent pipe, lymphocyte adsorbs on the glass fiber column layer, pour the cell mixed liquid in the reagent pipe through the discharging pipe, normal saline is added to the reagent pipe to the rethread inlet pipe, clear up the reagent pipe, add the eluant after the clearance is accomplished, the adsorbed lymphocyte is dissolved in the eluant on the glass fiber column layer, and easy operation is little to the cell harm, and the extraction volume is big.

Description

Leukemia lymphocyte separation and extraction device

Technical Field

The utility model relates to a lymphocyte draws technical field, concretely relates to leukemia lymphocyte separation and extraction device.

Background

The lymphocyte is one of the white blood cells, is the white blood cell with the smallest volume, is generated by a lymphatic organ, is mainly present in lymphatic fluid circulating in a lymphatic vessel, is an important cell component of an immune response function of an organism, is a main executor of almost all immune functions of a lymphatic system, is a first-line 'soldier' for resisting external infection and monitoring in-vivo cell variation, is a cell line with an immune recognition function, can be divided into a T lymphocyte, a B lymphocyte and a natural killer cell according to the difference of migration, surface molecules and functions, and is sometimes extracted from a medical experiment to perform observation and research.

The existing leukemia lymphocyte separation and extraction device has the disadvantages of complex and tedious operation, small extraction amount of lymphocytes and low extraction efficiency.

SUMMERY OF THE UTILITY MODEL

Therefore, the embodiment of the utility model provides a leukemia lymphocyte separation extraction element, it rotates to drive the pivot through motor work, thereby it rotates to drive the carousel, carry out centrifugal separation to the blood sample in the reagent pipe, lymphocyte adsorbs on the glass fiber column layer, pour the mixed liquid of cell in the reagent pipe through the discharging pipe, normal saline is added to the reagent pipe to the rethread inlet pipe, clear up the reagent pipe, add the eluant after the clearance is accomplished, adsorbed lymphocyte dissolves in the eluant on the glass fiber column layer, and the operation is thus simple, little to the cell damage, the extraction volume is big, with the complicated loaded down with trivial details of operation among the solution prior art, the problem that the lymphocyte extraction volume is few.

In order to achieve the above object, the embodiment of the present invention provides the following technical solutions: a leukemia lymphocyte separating and extracting device comprises a rotary table, wherein a rotary shaft is fixedly connected with the bottom of the rotary table, a motor is arranged at the bottom of the rotary table, an output shaft of the motor is fixedly connected with the bottom of the rotary shaft, and an extracting mechanism is arranged at the top of the rotary table;

it includes a plurality of reagent pipes, and is a plurality of the carousel top is all run through to reagent socle end, and is a plurality of the reagent socle top all is equipped with the screw thread lid, and is a plurality of the reagent pipe passes through threaded connection with relevant position's screw thread lid, and is a plurality of all be equipped with the rubber circle between reagent pipe and the relevant position's the screw thread lid, and is a plurality of the reagent intraduct all is equipped with the connecting rod, and is a plurality of the connecting rod top respectively with relevant position's screw thread lid bottom fixed connection, a plurality of the equal fixedly connected with glass fiber column layer in connecting rod bottom, a plurality of the equal fixedly connected with inlet pipe of reagent pipe one side, it is a plurality of the equal fixedly connected with discharging pipe of reagent pipe opposite side, it is a plurality of discharging pipe and inlet pipe all are located the carousel top, and are a plurality of the one end that inlet pipe and discharging.

Furthermore, a plurality of first through grooves are formed in the turntable, and the first through grooves are respectively located on two sides of the reagent tube at corresponding positions.

Further, it is a plurality of first logical inslot portion all is equipped with the spring, and is a plurality of spring one end all with carousel fixed connection.

Further, a plurality of the equal fixedly connected with rubber slab of spring other end, it is a plurality of the rubber slab contacts with the reagent pipe of relevant position respectively.

Further, a base is arranged between the rotary table and the motor, and one end of the rotating shaft penetrates through the base.

Furthermore, the sliding groove is formed in the top end of the base, the bottom end of the rotating disc is fixedly connected with two sliding columns, and the bottom ends of the two sliding columns extend into the sliding groove.

Further, four support columns are fixedly connected to the bottom end of the base, and the motor is located between the four support columns.

Furthermore, a plurality of second through grooves are formed in the rotary table, the second through grooves are respectively located at the bottom ends of the reagent tubes at corresponding positions, a temperature controller is arranged in each second through groove, and the temperature controller is fixedly connected with the rotary table.

The embodiment of the utility model provides a have following advantage:

the utility model discloses a motor work drives the pivot and rotates, thereby it rotates to drive the carousel, carry out centrifugal separation to the blood sample in the reagent pipe, lymphocyte adsorbs on the glass fiber column layer, pour the mixed liquid of cell in the reagent pipe through the discharging pipe, the normal saline is added to the reagent pipe to the rethread inlet pipe, clear up the reagent pipe, add the eluant after the clearance is accomplished, the adsorbed lymphocyte is dissolved in the eluant on the glass fiber column layer, compared with the prior art, the operation is thus simple, little to the cell harm, the extraction volume is big.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.

The structure, ratio, size and the like shown in the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by people familiar with the technology, and are not used for limiting the limit conditions which can be implemented by the present invention, so that the present invention has no technical essential significance, and any structure modification, ratio relationship change or size adjustment should still fall within the scope which can be covered by the technical content disclosed by the present invention without affecting the efficacy and the achievable purpose of the present invention.

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a sectional view of the overall structure of the present invention;

FIG. 3 is a cross-sectional view of the reagent vessel of the present invention;

FIG. 4 is an enlarged view of the structure A in FIG. 2 according to the present invention;

FIG. 5 is a perspective view of the base of the present invention;

in the figure: 1 carousel, 2 travellers, 3 bases, 4 support columns, 5 motors, 6 pivot, 7 connecting rods, 8 reagent pipes, 9 discharging pipes, 10 inlet pipes, 11 screw caps, 12 glass fiber column layers, 13 springs, 14 first logical groove, 15 rubber slab, 16 spouts, 17 second logical groove, 18 temperature controller.

Detailed Description

The present invention is described in terms of specific embodiments, and other advantages and benefits of the present invention will become apparent to those skilled in the art from the following disclosure. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to the attached drawings 1-5 of the specification, the leukemia lymphocyte separating and extracting device of the embodiment comprises a rotary table 1, a rotary shaft 6 is fixedly connected to the bottom end of the rotary table 1, a motor 5 is arranged at the bottom of the rotary table 1, an output shaft of the motor 5 is fixedly connected to the bottom end of the rotary shaft 6, an extracting mechanism is arranged at the top of the rotary table 1, the extracting mechanism comprises a plurality of reagent tubes 8, the bottom ends of the plurality of reagent tubes 8 penetrate through the top end of the rotary table 1, threaded covers 11 are arranged at the top ends of the plurality of reagent tubes 8, the plurality of reagent tubes 8 are in threaded connection with the threaded covers 11 at corresponding positions, rubber rings are arranged between the plurality of reagent tubes 8 and the threaded covers 11 at corresponding positions, connecting rods 7 are arranged inside the plurality of reagent tubes 8, the top ends of the plurality of connecting rods 7 are respectively fixedly connected to the bottom ends of the threaded covers 11 at corresponding positions, and glass fiber column layers 12 are fixedly connected, one side of each of the reagent tubes 8 is fixedly connected with a feeding tube 10, the other side of each of the reagent tubes 8 is fixedly connected with a discharging tube 9, the discharging tubes 9 and the feeding tubes 10 are positioned at the top of the turntable 1, nuts are arranged at the ends of the feeding tubes 10 opposite to the discharging tubes 9, and the feeding tubes 10 and the discharging tubes 9 are respectively in threaded connection with the nuts at corresponding positions;

further, a plurality of first through grooves 14 are formed in the turntable 1, and the plurality of first through grooves 14 are respectively located on two sides of the reagent tube 8 at corresponding positions;

further, springs 13 are arranged inside the first through grooves 14, and one ends of the springs 13 are fixedly connected with the rotary table 1;

furthermore, the other ends of the springs 13 are fixedly connected with rubber plates 15, and the rubber plates 15 are respectively contacted with the reagent tubes 8 at corresponding positions, so that the fixing effect is good;

furthermore, a base 3 is arranged between the turntable 1 and the motor 5, and one end of the rotating shaft 6 penetrates through the base 3, so that the supporting effect is good;

furthermore, a sliding groove 16 is formed in the top end of the base 3, two sliding columns 2 are fixedly connected to the bottom end of the rotary table 1, and the bottom ends of the two sliding columns 2 extend into the sliding groove 16, so that the stability is improved;

furthermore, the bottom end of the base 3 is fixedly connected with four support columns 4, and the motor 5 is positioned among the four support columns 4, so that the stability is improved;

furthermore, a plurality of second through grooves 17 are formed in the turntable 1, the second through grooves 17 are respectively located at the bottom ends of the reagent tubes 8 at corresponding positions, a temperature controller 18 is arranged in the second through grooves 17, and the temperature controller 18 is fixedly connected with the turntable 1 to adjust the temperature and facilitate lymphocyte separation.

The implementation scenario is specifically as follows: the staff adds the separation liquid into the reagent tube 8 first, then add the blood sample, the screw thread lid 11 is rotated to fix the screw thread lid 11 and the reagent tube 8, then the rubber ring is sleeved between the reagent tube 8 and the screw thread lid 11 to seal the reagent tube 8 and the screw thread lid 11, then the reagent tube 8 is put into the turntable 1, the reagent tube 8 moves in the turntable 1, when the reagent tube 8 contacts with the rubber plate 15, the rubber plate 15 is extruded by the reagent tube 8 to start to be away from the reagent tube 8, thereby compressing the spring 13, when the reagent tube 8 stops moving, the spring 13 rebounds to extrude the reagent tube 8 by the rubber plate 15, thereby fixing the reagent tube 8, the fixing effect is good, then the temperature controller 18 is started, the temperature of the bottom of the reagent tube 8 is always at the constant temperature of 37 ℃ when the temperature controller 18 works, the lymphocyte separation is convenient, then the motor 5 is started, the motor 5 works to drive the rotating shaft 6 to rotate, thereby driving the rotary table 1 to rotate, carrying out centrifugal separation on blood samples in the reagent tube 8, adsorbing lymphocytes on the glass fiber column layer 12, simultaneously, the rotary table 1 rotates to drive the sliding column 2 to move in the sliding groove 16, improving the stability of the rotary table 1, rotating uniformly, when the lymphocytes are adsorbed on the glass fiber column layer 12, taking out the reagent tube 8, opening the screw cap on the discharge tube 9, pouring the cell mixed liquid in the reagent tube 8, opening the screw cap on the feed tube 10, adding physiological saline into the reagent tube 8, cleaning the reagent tube 8, after cleaning, adding eluent into the reagent tube 8 through the feed tube 10, dissolving the lymphocytes adsorbed on the glass fiber column layer 12 into the eluent, separating and extracting the lymphocytes, having simple operation, little damage to cells, large extraction amount, being arranged in a plurality of groups of reagent tubes 8 at the same time, having high extraction efficiency, and particularly solving the complex and tedious operation in the prior art, the extraction amount of lymphocytes is small, and the extraction efficiency is low.

The working principle is as follows:

referring to fig. 1-5 of the specification, a worker first adds a separation solution and a blood sample to a reagent vessel 8, rotates a screw cap 11 to fix the screw cap 11 to the reagent vessel 8, then the reagent tube 8 is put into the rotary disk 1, the spring 13 rebounds to press the rubber plate 15 to the reagent tube 8, thereby fixing the reagent tube 8, starting the motor 5, driving the rotating shaft 6 to rotate by the motor 5, thereby driving the rotating disc 1 to rotate, the blood sample in the reagent tube 8 is centrifugally separated, the lymphocyte is adsorbed on the glass fiber column layer 12, when the lymphocyte is adsorbed on the glass fiber column layer 12, the cell mixed liquid in the reagent tube 8 is poured out through the discharging tube 9, and then the normal saline is added into the reagent tube 8 through the feeding tube 10, and cleaning the reagent tube 8, adding an eluent into the reagent tube 8 after the cleaning is finished, and dissolving the lymphocytes adsorbed on the glass fiber column layer 12 in the eluent.

Although the invention has been described in detail with respect to the general description and the specific embodiments, it will be apparent to those skilled in the art that modifications and improvements can be made based on the invention. Therefore, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. A leukemia lymphocyte separation and extraction device comprises a rotary disc (1), and is characterized in that: the bottom end of the rotary table (1) is fixedly connected with a rotating shaft (6), the bottom of the rotary table (1) is provided with a motor (5), an output shaft of the motor (5) is fixedly connected with the bottom end of the rotating shaft (6), and the top of the rotary table (1) is provided with an extraction mechanism;

the extraction mechanism comprises a plurality of reagent tubes (8), the bottom ends of the reagent tubes (8) penetrate through the top end of the turntable (1), the top ends of the reagent tubes (8) are provided with threaded covers (11), the reagent tubes (8) are in threaded connection with the threaded covers (11) of corresponding positions, rubber rings are arranged between the reagent tubes (8) and the threaded covers (11) of corresponding positions, connecting rods (7) are arranged inside the reagent tubes (8), the top ends of the connecting rods (7) are fixedly connected with the bottom ends of the threaded covers (11) of corresponding positions respectively, the bottom ends of the connecting rods (7) are fixedly connected with a glass fiber column layer (12), one sides of the reagent tubes (8) are fixedly connected with inlet tubes (10), the other sides of the reagent tubes (8) are fixedly connected with outlet tubes (9), and the outlet tubes (9) and the inlet tubes (10) are located at the top of the turntable (1), a plurality of one end that inlet pipe (10) and discharging pipe (9) carried on the back mutually all is equipped with the nut, and is a plurality of inlet pipe (10) and discharging pipe (9) respectively with relevant position's nut threaded connection.

2. The device for separating and extracting leukemia lymphocytes according to claim 1, wherein: a plurality of first through grooves (14) are formed in the turntable (1), and the first through grooves (14) are located on two sides of the reagent tube (8) at corresponding positions respectively.

3. The device for separating and extracting leukemia lymphocytes according to claim 2, wherein: a plurality of first logical groove (14) inside all is equipped with spring (13), and is a plurality of spring (13) one end all with carousel (1) fixed connection.

4. The device for separating and extracting leukemia lymphocytes according to claim 3, wherein: a plurality of equal fixedly connected with rubber slab (15) of spring (13) other end, it is a plurality of rubber slab (15) contact with reagent pipe (8) of relevant position respectively.

5. The device for separating and extracting leukemia lymphocytes according to claim 1, wherein: a base (3) is arranged between the turntable (1) and the motor (5), and one end of the rotating shaft (6) penetrates through the base (3).

6. The apparatus for separating and extracting leukemia lymphocytes according to claim 5, wherein: spout (16) have been seted up on base (3) top, two traveller (2) of carousel (1) bottom fixedly connected with, two inside traveller (2) bottom all extended into spout (16).

7. The apparatus for separating and extracting leukemia lymphocytes according to claim 5, wherein: four support columns (4) of base (3) bottom fixedly connected with, motor (5) are located between four support columns (4).

8. The device for separating and extracting leukemia lymphocytes according to claim 1, wherein: the reagent tube assembly is characterized in that a plurality of second through grooves (17) are formed in the turntable (1), the second through grooves (17) are located at the bottom ends of the reagent tubes (8) at corresponding positions respectively, a temperature controller (18) is arranged in each second through groove (17), and the temperature controller (18) is fixedly connected with the turntable (1).