CN211522209U

CN211522209U - Biochemical culture apparatus for producing laccase by coriolus versicolor in optimized environment

Info

Publication number: CN211522209U
Application number: CN201922151396.0U
Authority: CN
Inventors: 尚洁
Original assignee: North Minzu University
Current assignee: North Minzu University
Priority date: 2019-12-05
Filing date: 2019-12-05
Publication date: 2020-09-18
Anticipated expiration: 2029-12-05

Abstract

The utility model relates to an environment-optimized biochemical culture device for producing laccase by coriolus versicolor, which comprises a box body, wherein a box wall inner container is arranged on the inner side wall of the box body, a fixing frame is arranged on the upper end surface of the box wall inner container through an installation fixing block, a plurality of rows of fixing holes are longitudinally arranged on the fixing frame along the circumferential direction of the fixing frame, the number of each row of fixing holes is two or more, a culture dish placing tray is movably clamped on the fixing frame through a clamping rod which is arranged on the culture dish placing tray and matched with the fixing holes, a plurality of culture lamps are movably arranged on the box wall inner container, a gas circulation device is arranged below the inner side of the box wall inner container, a plurality of air pipes are arranged at the upper end of the gas circulation device, a high-low temperature conversion device is arranged on the inner side surface of the box wall inner container, the height of the culture dish placing tray, can provide a good and uniform temperature environment for laccase, and the laccase production rate is high.

Description

Biochemical culture apparatus for producing laccase by coriolus versicolor in optimized environment

The technical field is as follows:

the utility model relates to an environment-optimized biochemical culture device for producing laccase by Coriolus versicolor.

Background art:

the coriolus versicolor laccase production is that coriolus versicolor is selected as white rot fungus for producing laccase, the influence of initial pH, carbon source concentration, temperature, nitrogen source type and nitrogen source concentration of a culture medium on the fermentation of a wheat straw liquid culture medium to produce laccase is respectively researched, and on the basis of a single-factor test, the response surface is adopted to research the optimal conditions for producing the laccase by the coriolus versicolor.

The devices needed in the experimental process for producing laccase by coriolus versicolor comprise: the device comprises an ultraviolet-visible spectrophotometer, a biochemical incubator, an autoclave and an ultra-clean workbench, wherein the biochemical incubator is an important instrument in the process of producing laccase by coriolus versicolor, and the biochemical incubator needs to be kept stand and cultured in a dark place in the process of culturing laccase. However, the existing biochemical culture device for producing laccase by coriolus versicolor still has the problems that the culture dish placing plate is inconvenient to adjust, the culture dish placing is not fixed, the laccase is easy to shake and damage during taking and placing, a good and uniform temperature environment cannot be provided for the laccase, and the laccase production rate is low.

Therefore, the invention of the biochemical culture device for producing laccase by Coriolus versicolor with an optimized environment is very necessary.

The utility model has the following contents:

the utility model discloses a remedy prior art not enough, provide a biochemical culture apparatus of laccase is produced to coriolus versicolor who optimizes the environment, it conveniently adjusts the culture dish and places a set height, and the culture dish is placed fixedly, is difficult to rock when getting and destroys the laccase production, can provide a good even temperature environment for laccase, and the laccase production rate is high, has solved the problem that exists among the prior art.

The utility model discloses a solve the technical scheme that above-mentioned technical problem adopted and be:

the biochemical culture device comprises a box body, wherein a box wall inner container is arranged on the inner side wall of the box body, a fixing frame is arranged on the upper end face of the box wall inner container through an installation fixing block, a plurality of rows of fixing holes are longitudinally formed in the fixing frame along the circumferential direction of the fixing frame, the number of each row of fixing holes is two or more, a culture dish placing disc is movably clamped on the fixing frame through a clamping rod which is arranged on the culture dish placing disc and matched with the fixing holes, a plurality of culture lamps are movably arranged on the box wall inner container, a gas circulating device is arranged below the inner side of the box wall inner container, a plurality of air pipes are arranged at the upper end of the gas circulating device, a high-low temperature conversion device is arranged on one side face of the inner side of the box wall inner container, and the constant temperature device adopts the model of HD.

Be provided with the sucking disc that a plurality of materials are silica gel on the inside up end of placing the dish at the culture dish, the diameter of every sucking disc all sets up to 2.4cm, all inhales the culture dish that has circular glass material in the top of every sucking disc, and the bottom of every culture dish all is provided with the diameter and is 2.5cm and the degree of depth is 0.5cm inhales the groove.

And a plurality of air outlets are formed in the peripheral surface of each air pipe, and each air pipe surrounds the inner container surface of the box wall and is fixed through a buckle.

The cultivation lamp all adopts circular LED to cultivate the lamp, and every cultivation lamp all imbeds on the tank wall inner bag face, is connected with the baffle at the inboard one end of just cultivateing the lamp, installs rotatory fixed the knot in the baffle bottom, cultivates the lamp and drives the slip gear through manual application of force and slide, cultivates the lamp and slides the back and detain fixedly through rotating rotatory fixed.

The outer side of the box body is provided with a box door through a hinge, an observation window is arranged on the surface of the box door, and the outer side of the box door is connected with a handle through a bolt.

By adopting the scheme, the utility model, through installing the culture dish placing disc on the fixing frame, the culture dish placing disc can be disassembled through pulling, through pushing, embedding and installing, the culture dish placing disc is convenient to install and disassemble for disinfection, the culture dish placing disc is convenient to fix, the height of the culture dish placing disc can be adjusted according to needs, and the culture dish placing disc is firm and convenient to use; the culture dish is fixed through the sucking disc, and be favorable to conveniently placing, and the setting of sucking disc does benefit to and prevents that the inside laccase of culture dish from rocking the production that arouses destruction laccase placing the in-process, provides good condition of stewing for the laccase, improves the output of laccase, and the tuber pipe distributes on the global all around of tank wall inner bag, can effectively make the inside air normal cycle of tank wall inner bag, and be favorable to making inside temperature even, provides the production environment of laccase, makes laccase have a good temperature environment, promotes the production of laccase, improves the whole quality of laccase.

Description of the drawings:

fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic sectional structure diagram of the present invention.

Fig. 3 is a schematic view of the fixing frame of the present invention.

Fig. 4 is a schematic view of the top view structure of the culture dish placing plate of the present invention.

Fig. 5 is a schematic view of the cross-sectional structure of the culture dish placing tray of the present invention.

Fig. 6 is a schematic view of the structure of the culture dish of the present invention.

Fig. 7 is a schematic view of the cross-sectional enlarged structure at a of the present invention.

In the figure, 1, a box body, 2, a box wall inner container, 3, a mounting and fixing block, 4, a fixing frame, 5, a fixing hole, 6, a fixing block, 7, a culture dish placing plate, 71, a transparent disc, 72, a sucking disc, 73, a clamping rod, 74, a culture dish, 741, a shell, 742, a sucking groove, 743, a sealing cover, 8, a culture lamp, 81, a baffle, 82, a sliding gear, 83, a rotary fixing buckle, 9, a gas circulation device, 10, an air pipe, 11, a constant temperature device, 12, a high and low temperature conversion device, 13, a box door, 14, a glass observation window, 15, a box door handle and 16 moving wheels.

The specific implementation mode is as follows:

in order to clearly illustrate the technical features of the present invention, the present invention is explained in detail by the following embodiments in combination with the accompanying drawings.

As shown in fig. 1 to 7, a biochemical culture device for producing laccase from coriolus versicolor for environment optimization comprises a box body 1, a box wall inner container 2 is arranged on the inner side wall of the box body 1, a fixing frame 4 is arranged on the upper end surface of the box wall inner container 2 through a mounting fixing block 3, a plurality of rows of fixing holes 5 are longitudinally arranged on the fixing frame 4 along the circumferential direction of the fixing frame 4, the number of each row of fixing holes 5 is two or more, a culture dish placing disc 7 is movably clamped on the fixing frame 4 through a clamping rod 73 which is arranged on the culture dish placing disc and matched with the fixing holes 5, a plurality of culture lamps 8 are movably arranged on the box wall inner container 2, a gas circulation device 9 is arranged below the inner side of the box wall inner container 2, a plurality of air pipes are arranged at the upper end of the gas circulation device 9, a high-low temperature conversion device 12 is arranged on one side surface of the inner side surface of the box wall inner container, the work of disinfecting is dismantled in easy to assemble, and the dish is placed to convenient fixed culture dish, can place the height of dish and adjust as required to the culture dish, and is fixed durable, facilitates the use.

Be provided with the sucking disc 72 that a plurality of materials are silica gel on the inside up end of placing dish 7 at the culture dish, the diameter of every sucking disc 72 all sets up to 2.4cm, culture dish 74 that circular glass material has all been inhaled in the top of every sucking disc 72, the bottom of every culture dish 74 all is provided with the diameter and is 2.5cm and the degree of depth is 0.5 cm's suction groove 742, all be provided with closing cap 743 in the upper end of every culture dish 74, all be provided with the closing cap in the upper end of every culture dish, the culture dish is fixed through the sucking disc, be favorable to conveniently placing, and the setting of sucking disc does benefit to and prevents that the inside laccase of culture dish from rocking the production that arouses destruction laccase in placing the process, provide good condition of standing for laccase, improve the output of laccase.

The peripheral surface of each air pipe 10 is provided with a plurality of air outlets, each air pipe 10 is surrounded on the surface of the tank wall inner container 2 and is fixed through a buckle, air inside the tank wall inner container can be effectively circulated normally, the internal temperature is uniform, a laccase generating environment is provided, the laccase has a good temperature environment, the generation of the laccase is promoted, and the integral quality of the laccase is improved.

Cultivate lamp 8 and all adopt circular LED to cultivate the lamp, every is cultivateed lamp 8 and all imbeds on 2 faces of tank wall inner bag, is connected with baffle 81 in the inboard one end of cultivateing lamp 8, installs rotatory fixed knot 83 in baffle 81 bottom, cultivates lamp 8 and drives sliding gear 82 through manual application of force and slide, cultivates lamp 8 and slides the back and detains 83 fixed convenient removal through rotating rotatory fixed, can adjust the position of cultivateing the lamp as required, obtain the optimum temperature in the messenger laccase production, improve laccase output.

The outer side of the box body 1 is provided with a box door 13 through a hinge, an observation window 14 is arranged on the surface of the box door 13, the outer side of the box door 13 is connected with a handle 15 through a bolt, the inner condition can be conveniently detected through the observation window, and the laccase production condition can be conveniently checked at any time.

When the refrigerator is used, the outside of the refrigerator body 1 and the inside of the tank wall liner 2 are cleaned, the fixed clamping block 6 at one end of the fixed frame 4 is taken out from the groove of the fixed block 3 for cleaning and then is installed, after the cleaning is finished, the refrigerator door 13 is closed, the power supply is switched on to open the high-low temperature conversion device 12, the internal temperature of the tank wall liner 2 gradually reaches a set value, and after the temperature is proper, the power supply is temporarily closed; opening a sealing cover 743 at one end of a culture dish 74, manually placing the culture dish 74 on one end face of a culture dish placing plate 7, sucking a sucking disc 72 in a sucking groove 742 at the bottom end of the culture dish 74 for fixation, placing Coriolus versicolor in the culture dish 74, covering the sealing cover 743, lightly fixing the culture dish placing plate 7 on one side face of a fixing frame 4, inserting a clamping rod 73 into a fixing hole 5 for fixation as required during fixation, and closing a box door 13 after fixation; the device is powered on, the high-temperature and low-temperature conversion device 12 is turned on through a power button to set the temperature required by the laccase, when the temperature in the box body 1 reaches a set value, the constant temperature device 11 is turned on to enable the temperature in the box body 1 to be in a balanced state, then the gas circulation device 9 is turned on, the gas circulation device 9 conveys gas to the interior of the air pipe 10 and then the gas is discharged from a plurality of air outlet controls on one side of the air pipe 10, so that the temperature in the box body is uniform, a laccase generation environment is provided, the laccase has a good temperature environment, the generation of the laccase is promoted, and the overall quality of the laccase is improved.

The above-mentioned specific embodiments can not be regarded as the restriction to the scope of protection of the utility model, to technical personnel in this technical field, it is right the utility model discloses any replacement improvement or transform that embodiment made all fall within the scope of protection of the utility model.

The parts of the present invention not described in detail are the known techniques of those skilled in the art.

Claims

1. The biochemical culture device for producing laccase by Coriolus versicolor for optimizing environment is characterized in that: the incubator comprises a box body, an inner wall of the box body is provided with an inner wall of the box body, a fixing frame is arranged on the upper end face of the inner wall of the box body through an installation fixing block, a plurality of rows of fixing holes are longitudinally formed in the fixing frame along the circumferential direction of the fixing frame, the number of each row of fixing holes is two or more, a culture dish placing plate is movably clamped on the fixing frame through clamping rods which are arranged on the culture dish placing plate and matched with the fixing holes, a plurality of culture lamps are movably arranged on the inner wall of the box body, a gas circulating device is arranged below the inner side of the inner wall of the box body, a plurality of air pipes are arranged at the upper end of the gas circulating device, a high-low temperature conversion device is arranged on one side face of the.

2. The environment-optimized biochemical culture device for the coriolus versicolor laccase production according to claim 1, wherein the environment-optimized biochemical culture device comprises: be provided with the sucking disc that a plurality of materials are silica gel on the inside up end of placing the dish at the culture dish, the diameter of every sucking disc all sets up to 2.4cm, all inhales the culture dish that has circular glass material in the top of every sucking disc, and the bottom of every culture dish all is provided with the diameter and is 2.5cm and the degree of depth and be 0.5cm inhales the groove, all is provided with the closing cap in the upper end of every culture dish.

3. The biochemical culture device for the coriolus versicolor laccase production with the optimized environment according to claim 1 or 2, wherein the biochemical culture device comprises: and a plurality of air outlets are formed in the peripheral surface of each air pipe, and each air pipe surrounds the inner container surface of the box wall and is fixed through a buckle.

4. The environment-optimized biochemical culture device for the coriolus versicolor laccase production according to claim 1, wherein the environment-optimized biochemical culture device comprises: the cultivation lamp all adopts circular LED to cultivate the lamp, and every cultivation lamp all imbeds on the tank wall inner bag face, is connected with the baffle in the inboard one end of cultivation lamp, installs rotatory fixed knot in the baffle bottom, cultivates the lamp and drives the slip gear through manual application of force and slide, cultivates the lamp and slides the back and detain fixedly through rotating rotatory fixed.

5. The environment-optimized biochemical culture device for the coriolus versicolor laccase production according to claim 1, wherein the environment-optimized biochemical culture device comprises: the outer side of the box body is provided with a box door through a hinge, an observation window is arranged on the surface of the box door, and the outer side of the box door is connected with a handle through a bolt.