CN211505222U

CN211505222U - Fluorescence immunoassay appearance

Info

Publication number: CN211505222U
Application number: CN201921926392.9U
Authority: CN
Inventors: 林华青; 李俊玲; 易展飞; 甄克敏; 阮云艳
Original assignee: Zhuhai Dl Medical Biotech Co ltd
Current assignee: Zhuhai deer Bioengineering Co.,Ltd.
Priority date: 2019-11-08
Filing date: 2019-11-08
Publication date: 2020-09-15
Anticipated expiration: 2029-11-08

Abstract

The utility model relates to an immunology detects the field, discloses a fluorescence immunoassay appearance, including the storehouse body, the internal reaction unit that sets up in storehouse, magnetism adsorption equipment, fluorescence detection device and reagent transfer device, reaction unit holds portion and reagent reaction portion including reagent, reagent transfer device includes the straw, the head of straw can stretch into reagent and hold in portion or the reagent reaction portion with absorption or release reagent, magnetism adsorption equipment can adsorb the compound that has magnetic field response that the reaction back generated in the reagent reaction portion in the bottom of reagent reaction portion, fluorescence detection device is used for detecting the fluorescence intensity that has the compound of magnetic field response. The analyzer is fast and simple to operate, the condition of reagent overturn or reagent error addition cannot occur, the separation and the fluorescence detection of the compound with the magnetic field response are completed in one set of equipment, the influence on the separation process of the compound is reduced, and the separation effect of the compound and the accuracy of the fluorescence detection result are improved.

Description

Fluorescence immunoassay appearance

Technical Field

The utility model relates to an immunology detects the field, especially relates to a fluorescence immunoassay appearance.

Background

The fluorescence immunoassay technology is the earliest developed technology in the labeled immunoassay technology, and is a technology for labeling an antibody with a fluorescent substance to perform antigen localization. In some existing fluorescence immunoassay techniques, after various reagents are mixed with a sample to be detected for reaction, the sample to be detected is cleaved to generate substances required for fluorescence immunoassay, such as nucleic acid. The substance can continuously react with the reagent to combine and generate a complex with magnetic field response, and after the complex with magnetic field response is separated, the concentration of the substance required by the fluorescence immunoassay in the sample to be detected can be obtained by detecting the fluorescence intensity of the complex with magnetic field response.

In the prior art, a plurality of reagents are added into a sample to be detected by a worker, the sample is placed into an incubation chamber for incubation for a certain time, then a compound with magnetic field response is separated out, and finally the fluorescence intensity is detected on a detection platform. The whole process is complex to operate, the separation process of the compound is affected, the detection speed is low, and the detection result is easy to have large deviation. In addition, in the operation process, the situations of reagent overturn, reagent adding mistake and the like are easy to happen to workers, so that waste is caused or a correct diagnosis result cannot be obtained.

SUMMERY OF THE UTILITY MODEL

For solving one of the technical problem that exists among the prior art at least, the utility model provides a fluorescence immunoassay appearance, its operation is quick simple and convenient, can improve the accuracy of testing result.

The utility model provides a technical scheme that its technical problem adopted does:

a fluorescence immunoassay instrument comprises a bin body, wherein a reaction device, a magnetic adsorption device and a fluorescence detection device are sequentially arranged along the X-axis direction below the interior of the bin body, a reagent transfer device is arranged above the interior of the bin body, the reaction device is detachably and slidably mounted in the bin body, the reaction device is arranged along the Y-axis direction in a plurality and can slide along the X-axis direction, the reaction device comprises a reagent containing part and a reagent reaction part, the reagent transfer device can move along the X-axis direction and the Z-axis direction, the reagent transfer device comprises a straw, the head of the straw can extend into the reagent containing part or the reagent reaction part to absorb or release a reagent, the magnetic adsorption device can move along the Z-axis direction to be close to the bottom of the reagent reaction part, and a compound with magnetic field response generated after reaction in the reagent reaction part can be adsorbed at the bottom of the reagent reaction part, the fluorescence detection device is used for detecting the fluorescence intensity of the complex with the magnetic field response.

Has the advantages that: when the analyzer is used for fluorescence immunoassay, reagents required by various reactions are placed in the reagent containing part, placing a sample to be tested in the reagent reaction part, then installing the reaction device in the chamber body, extending a suction pipe on the reagent transfer device into the reagent containing part to suck the reagent, and the reagent is transferred into the reagent reaction part to be released, after various reagents react with the sample to be tested, the magnetic adsorption device is lifted to be close to the bottom of the reagent reaction part along the Z-axis direction, the compound with magnetic field response generated by the reaction is adsorbed at the bottom of the reagent reaction part, the suction pipe extends into the reagent reaction part to suck waste liquid, transferring the waste liquid to a reagent containing part, sucking the detergent from the reagent containing part to the reagent reaction part for releasing, and (3) washing the compound with the magnetic field response, and repeating the operation for multiple times to separate the compound with the magnetic field response with higher purity. Because the reaction device is provided with a plurality of groups, a plurality of groups of operations can be carried out simultaneously, and a plurality of groups of compounds with magnetic field response are obtained by separation. After the compound with the magnetic field response is separated, the magnetic adsorption device descends along the Z-axis direction to give way, the reaction device moves along the X-axis direction to be close to the fluorescence detection device, and the fluorescence detection device detects the fluorescence intensity of the compound. The analyzer is fast and simple to operate, the reagent transfer device completes the operation of adding the reagent, and the condition of reagent overturn or reagent adding error can not occur. And the separation and the fluorescence detection of the compound with the magnetic field response are finished in one set of equipment, so that the influence on the separation process of the compound is greatly reduced, and the separation effect of the compound and the accuracy of a fluorescence detection result are improved.

As an improvement of the technical scheme, the reaction device further comprises a straw containing part, the reagent containing part and the reagent reaction part are distributed along the X-axis direction, and the reagent transfer device can take and place the straw from the straw containing part. The straw holds and has placed unused new straw in the portion, and reaction unit installs into the storehouse internal back, holds the portion interior straw of taking from the straw by reagent transfer device, and after the reaction was accomplished and was extracted the complex that has magnetic field response, reagent transfer device will make used straw put back the straw again and hold in the portion to in the change is used, can avoid reagent to pollute.

As an improvement of the above technical solution, the magnetic adsorption means includes a magnet. When the magnetic adsorption device rises along the Z-axis direction, the magnet is close to the bottom of the reagent reaction part, so that the compound with magnetic field response can be adsorbed at the bottom of the reagent reaction part, and when the suction pipe extends into the reagent reaction part to suck waste liquid, the compound with magnetic field response is tightly attached to the bottom of the reagent reaction part and cannot be sucked into the suction pipe.

As an improvement of the technical scheme, the reaction device is connected with the bin body in a sliding mode through a tray, a plurality of sliding grooves are formed in the tray, and the reaction device is detachably connected with the sliding grooves through sliding blocks. After the required reagent and the sample to be detected are placed in the reaction device, the reaction device can be installed on the sliding groove, and then the tray is pushed, so that the tray drives the reaction device to enter the warehouse body, and the subsequent steps can be conveniently carried out. After the analysis and detection are finished, the tray drives the reaction device to slide out of the bin body so as to be convenient for replacing a new reaction device.

As an improvement of the technical scheme, the tray is provided with a limiting part for limiting the moving range of the reaction device. The limiting part can prevent the reaction device from sliding out of the tray when the reaction device is installed, and can also prevent the reaction device from sliding when reagent is transferred.

As an improvement of the technical scheme, the reagent transfer device further comprises a first Z-axis moving plate, a second Z-axis moving plate is connected to the first Z-axis moving plate in a sliding mode, the second Z-axis moving plate is connected with the tail portion of the piston rod, and the head portion of the piston rod is connected with the tail portion of the suction tube in a matched mode. When reagent shifts, descend by first Z axle movable plate earlier for the head of straw stretches into in reagent holds the portion or the reagent reaction portion, and the head that drives the piston rod by second Z axle movable plate again rises or descends, makes the head of piston rod aspirate in the cavity of straw afterbody, thereby realizes the absorption or the release of reagent, utilizes the cooperation motion between piston rod and the straw, and under the effect of atmospheric pressure, reagent will not drip and spill, thereby avoids the storehouse body or tray to be corroded by reagent.

As an improvement of the technical scheme, a constant temperature system is further arranged in the bin body. Under the action of the constant temperature system, the reaction between the reagent and the sample to be detected can be facilitated, so that the sample to be detected can be fully cracked, and a compound with magnetic field response required by fluorescence detection can be generated in a combined manner.

Drawings

The present invention will be further described with reference to the accompanying drawings and specific embodiments, wherein:

fig. 1 is a schematic overall structure diagram of an embodiment of the present invention;

fig. 2 is a partial structural schematic diagram of an embodiment of the present invention;

FIG. 3 is a diagram showing the positional relationship between the reaction device and the magnetic adsorption device according to the embodiment of the present invention;

FIG. 4 is a schematic structural diagram of a reaction apparatus according to an embodiment of the present invention;

FIG. 5 is a schematic view of a part of a reaction apparatus according to an embodiment of the present invention;

FIG. 6 is a schematic view of a part of the structure of a fluorescence detection device according to an embodiment of the present invention;

fig. 7 is a schematic view of a partial structure of a reagent transfer device according to an embodiment of the present invention.

In the drawings: 1 storehouse body, 2 reaction unit, 3 magnetic adsorption device, 4 fluorescence detection device, 5 reagent transfer device, 6 trays, 11 first motors, 12 second motors, 13 third motors, 14 fourth motors, 15 fifth motors, 16 first X axle guide rail, 17 second X axle guide rail, 21 reagent holds the portion, 22 reagent reaction portion, 23 straw holds the portion, 24 slider, 31 magnet, 41 transmission optical assembly, 42 receives optical assembly, 43 sweeps a yard subassembly, 44 light intensity detection subassembly, 45 radiator unit, 51 straw, 52 first Z axle movable plate, 521 second Z axle movable plate, 522 piston rod, 523 sixth motor, 61 spout, 62 spacing portion.

Detailed Description

Referring to fig. 1 and 2, an embodiment of the present invention provides a fluorescence immunoassay analyzer, which includes a chamber body 1, a reaction device 2, a magnetic adsorption device 3 and a fluorescence detection device 4 are sequentially disposed in the chamber body 1 along an X-axis direction at a lower portion, a reagent transfer device 5 is disposed in the chamber body 1 at an upper portion, the reaction device 2 is slidably mounted in the chamber body 1 in a detachable manner, the reaction device 2 is arranged in a plurality along the Y-axis direction and can slide along the X-axis direction, in this embodiment, the reaction device 2 includes 6 reagent holding portions 21 and reagent reaction portions 22, the reagent transfer device 5 can move along the X-axis direction and the Z-axis direction, the reagent transfer device 5 includes a suction tube 51, a head portion of the suction tube 51 can extend into the reagent holding portion 21 or the reagent reaction portion 22 to suck or release a reagent, the magnetic adsorption device 3 can move along the Z-axis direction to be close to a bottom portion of the reagent reaction portion 22, the magnetic adsorption means 3 can adsorb the compound having a magnetic field response generated after the reaction in the reagent reaction part 22 to the bottom of the reagent reaction part 22, and the fluorescence detection means 4 is used to detect the fluorescence intensity of the compound having a magnetic field response. In this embodiment, the storehouse body 1 is provided with three stations along the Y axle direction, all is provided with reaction unit 2, magnetic adsorption device 3 and reagent transfer device 5 on each station correspondingly, and fluorescence detection device 4 then can only set up a set of, can reduce economic cost, and under the drive of third motor 13, fluorescence detection device 4 can be followed the Y axle direction and removed to carry out fluorescence detection at the station of difference.

Referring to fig. 2, when the fluorescence immunoassay analyzer is used, reagents required for various reactions are placed in the reagent containing part 21, a sample to be measured is placed in the reagent reaction part 22, and then the reaction device 2 is installed in the cartridge body 1. Referring to fig. 2, a first X-axis guide rail 16 is arranged in the bin body 1, the first X-axis guide rail 16 is slidably connected to the reagent transferring device 5, the reagent transferring device 5 is driven by a fourth motor 14 to move along the first X-axis guide rail 16, and then the reagent transferring device 5 is driven by a fifth motor 15 to move along the Z-axis direction, so that a suction pipe 51 on the reagent transferring device 5 extends into the reagent containing part 21 to suck the reagent, and the reagent is transferred into the reagent reaction part 22 to be released. In this embodiment, the magnetic adsorption device 3 includes a magnet 31, after various reagents react with a sample to be tested, the magnetic adsorption device 3 is driven by the second motor 12 to ascend along the Z-axis direction, the magnet 31 is close to the bottom of the reagent reaction part 22 to adsorb a compound with magnetic field response generated by the reaction at the bottom of the reagent reaction part 22, the test pipette 51 is inserted into the reagent reaction part 22 to aspirate waste liquid and transfer the waste liquid to the reagent containing part 21, and then aspirate a detergent from the reagent containing part 21 into the reagent reaction part 22 to be released, and the compound with magnetic field response is washed. Because the reaction device 2 is provided with a plurality of groups, a plurality of groups of operations can be carried out simultaneously, and a plurality of groups of compounds with required magnetic field response can be separated and obtained simultaneously.

Referring to fig. 2 and 6, after the compound with magnetic field response is separated, the second motor 12 drives the magnetic adsorption device 3 to lower and give way along the Z-axis direction, the first motor 11 drives the reaction device 2 to move along the second X-axis guide rail 17 to approach the fluorescence detection device 4, and the fluorescence detection device 4 detects the fluorescence intensity of the compound. In this embodiment, the fluorescence detection device 4 includes an emitting light component 41, a receiving light component 42, a code scanning component 43, a light intensity detection component 44, and a heat dissipation component 45, where the emitting light component 41 is used to excite fluorescence, the receiving light component 42 receives the fluorescence and then transmits the fluorescence to the light intensity detection component 44 to perform light intensity detection, the code scanning component 43 can identify a barcode on the reaction device 2 to obtain information of the reaction device 2, and the heat dissipation component 45 can prevent the local temperature of the fluorescence detection device 4 from being too high and causing adverse effects on the detection result. The analyzer is fast and simple to operate, the reagent transfer device 5 completes the reagent adding operation, and the condition of reagent overturn or reagent adding error can not occur. And the separation and the fluorescence detection of the compound with the magnetic field response are finished in one set of equipment, so that the influence on the separation process of the compound is greatly reduced, and the separation effect of the compound and the accuracy of a fluorescence detection result are improved.

Referring to fig. 3, 4 and 5, in some embodiments, the reaction device 2 further includes a pipette containing portion 23, a reagent containing portion 21 and a reagent reaction portion 22, which are sequentially distributed along the X axis, a new unused pipette 51 is placed in the pipette containing portion 23, after the reaction device 2 is installed in the chamber 1, the pipette 51 is taken out from the pipette containing portion 23 by the reagent transferring device 5, and after the reaction is completed and the compound with magnetic field response is extracted, the used pipette 51 is placed back into the pipette containing portion 23 by the reagent transferring device 5 for replacement and use, so as to prevent reagent contamination.

Referring to fig. 3, 4 and 5, in some embodiments, the reaction device 2 is slidably connected to the bin body 1 through the tray 6, under the driving of the first motor 11, the tray 6 can bear the reaction device 2 to move along the second X-axis guide rail 17, the tray 6 is provided with a plurality of chutes 61, the reaction device 2 is detachably connected to the chutes 61 through the sliders 24, after the required reagent and the sample to be detected are placed in the reaction device 2, the reaction device 2 can be installed on the chutes 61, and then the tray 6 is pushed to drive the reaction device 2 to enter the bin body 1 by the tray 6, so as to facilitate the subsequent steps. After the analysis and detection are finished, the tray 6 drives the reaction device 2 to slide out of the bin body 1, so that the new reaction device 2 can be replaced. The tray 6 is provided with a limit portion 62 for limiting the moving range of the reaction device 2, so that the reaction device 2 can be prevented from sliding out of the tray 6 when the reaction device 2 is installed, and the reaction device 2 can be prevented from sliding when reagent is transferred.

Referring to fig. 7, in some embodiments, the reagent transferring device 5 further comprises a first Z-axis moving plate 52, a second Z-axis moving plate 521 is slidably connected to the first Z-axis moving plate 52, the second Z-axis moving plate 521 is connected to the tail of a piston rod 522, and the head of the piston rod 522 is fittingly connected to the tail of the pipette 51. When reagent is transferred, the fifth motor 15 drives the first Z-axis moving plate 52 to descend, so that the head of the straw 51 extends into the reagent containing part 21 or the reagent reaction part 22, and the sixth motor 523 drives the second Z-axis moving plate 521 to drive the head of the piston rod 522 to ascend or descend, so that the head of the piston rod 522 is sucked in the cavity at the tail part of the straw 51, thereby sucking or releasing the reagent, and by utilizing the matching motion between the piston rod 522 and the straw 51, the reagent cannot drip out under the action of air pressure, thereby preventing the bin body 1 or the tray 6 from being corroded by the reagent. In addition, the storehouse body 1 is provided with a constant temperature system, in some embodiments, a heat source can be arranged in the storehouse body 1 to heat the air in the whole storehouse body 1, so that the air in the storehouse body 1 is in a constant temperature state, and under the action of the constant temperature system, the reaction between the reagent and the sample to be detected can be facilitated, so that the sample to be detected can be fully cracked, and a compound with magnetic field response required by fluorescence detection can be obtained by combination.

It should be noted that all the directional indicators (such as upper, lower, left, right, front and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indicator is changed accordingly. In addition, descriptions such as "first", "second", and the like in the embodiments of the present invention are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

The above description is only a preferred embodiment of the present invention, but the present invention is not limited to the above embodiments, and the technical effects of the present invention should be all included in the protection scope of the present invention as long as the technical effects are achieved by any of the same or similar means.

Claims

1. A fluorescence immunoassay analyzer is characterized in that: comprises a bin body (1), a reaction device (2), a magnetic adsorption device (3) and a fluorescence detection device (4) are sequentially arranged at the lower part in the bin body (1) along the X-axis direction, a reagent transfer device (5) is arranged at the upper part in the bin body (1), the reaction device (2) is detachably and slidably mounted in the bin body (1), the reaction device (2) is arranged along the Y-axis direction and can slide along the X-axis direction, the reaction device (2) comprises a reagent containing part (21) and a reagent reaction part (22), the reagent transfer device (5) can move along the X-axis direction and the Z-axis direction, the reagent transfer device (5) comprises a straw (51), the head part of the straw (51) can extend into the reagent containing part (21) or the reagent reaction part (22) to absorb or release the reagent, the magnetic adsorption device (3) can move along the Z-axis direction to be close to the bottom of the reagent reaction part (22), the magnetic adsorption device (3) can adsorb the compound with magnetic field response generated after reaction in the reagent reaction part (22) to the bottom of the reagent reaction part (22), and the fluorescence detection device (4) is used for detecting the fluorescence intensity of the compound with magnetic field response.

2. The fluoroimmunoassay analyzer of claim 1, wherein: reaction unit (2) still include the straw and hold portion (23), the straw holds portion (23), reagent and holds portion (21) and reagent reaction portion (22) and distribute along the X axle direction, reagent transfer device (5) can hold portion (23) from the straw and go up to get and put straw (51).

3. The fluoroimmunoassay analyzer of claim 1, wherein: the magnetic adsorption device (3) comprises a magnet (31).

4. The fluoroimmunoassay analyzer of claim 1, wherein: the reaction device (2) is connected with the bin body (1) in a sliding mode through a tray (6), a plurality of sliding grooves (61) are formed in the tray (6), and the reaction device (2) is detachably connected with the sliding grooves (61) through a sliding block (24).

5. The fluoroimmunoassay analyzer of claim 4, wherein: the tray (6) is provided with a limiting part (62) for limiting the moving range of the reaction device (2).

6. The fluoroimmunoassay analyzer of claim 1, wherein: the reagent transfer device (5) further comprises a first Z-axis moving plate (52), a second Z-axis moving plate (521) is connected to the first Z-axis moving plate (52) in a sliding mode, the second Z-axis moving plate (521) is connected with the tail portion of the piston rod (522), and the head portion of the piston rod (522) is connected with the tail portion of the sucker (51) in a matched mode.

7. The fluoroimmunoassay analyzer according to any one of claims 1 to 6, wherein: a constant temperature system is also arranged in the bin body (1).