CN211503427U - Aseptic liquid nitrogen system - Google Patents

Abstract

The utility model discloses an aseptic liquid nitrogen system, which comprises a nitrogen generating device used for generating nitrogen; the sterilizing device is matched with the nitrogen generating device through a first pipeline and is used for sterilizing the nitrogen conveyed by the first pipeline; and the liquid nitrogen generating device is matched with the sterilizing device through a second pipeline and is used for compressing the sterilized nitrogen to form sterile liquid nitrogen. The liquid nitrogen generated by the utility model is sterile and nontoxic, and when the cells are frozen and cultured, the cells are not polluted, so that the survival rate of the cells is guaranteed; and the sterile liquid nitrogen has wide application range and can meet the requirements of various fields.

Description

Aseptic liquid nitrogen system

Technical Field

The utility model belongs to the technical field of biotechnology, especially, relate to an aseptic liquid nitrogen system.

Background

The liquid nitrogen is widely used in the biological field for cell culture, embryo freezing and other technologies due to the characteristics of stability, no toxicity and low temperature under normal pressure; at present, liquid nitrogen produced by biological laboratories and the like is directly purchased from the market, and manufacturers for producing the liquid nitrogen load the liquid nitrogen into a tank body and then transport the liquid nitrogen to required laboratories or places needing to use the liquid nitrogen.

However, since the directly purchased liquid nitrogen is produced by other manufacturers, and the produced liquid nitrogen is not only applied to the field of biology, the requirements on the sterility and the non-toxicity of the liquid nitrogen in the production are not strict, the produced liquid nitrogen is easy to cause germ pollution, and meanwhile, the liquid nitrogen is easy to be polluted by a storage tank and a pipeline for conveying nitrogen when the liquid nitrogen is filled and conveyed, so most of the directly purchased liquid nitrogen cannot reach the sterile and non-toxic conditions required by the biological manufacture, and cells are easy to be polluted.

SUMMERY OF THE UTILITY MODEL

The utility model provides a system for producing sterile and nontoxic sterile liquid nitrogen, which overcomes the defects of the prior art.

In order to achieve the above purpose, the utility model adopts the following technical scheme: a sterile liquid nitrogen system comprises

The nitrogen generating device is used for generating nitrogen;

the sterilizing device is matched with the nitrogen generating device through a first pipeline and is used for sterilizing the nitrogen conveyed by the first pipeline;

and the liquid nitrogen generating device is matched with the sterilizing device through a second pipeline and is used for compressing the sterilized nitrogen to form sterile liquid nitrogen.

The nitrogen generator generates nitrogen, the nitrogen is conveyed to the sterilizing device through the first pipeline for sterilization and disinfection, and the nitrogen after sterilization and disinfection is conveyed to the liquid nitrogen generator through the second pipeline to be compressed into liquid nitrogen; the nitrogen generated by the nitrogen generating device is filtered and sterilized, so that the purity of the nitrogen is high, and liquid nitrogen formed after the nitrogen is compressed has a sterile and nontoxic effect, so that cells are not polluted when being frozen and cultured, and the survival rate of the cells is guaranteed; meanwhile, the liquid nitrogen produced by the sterile liquid nitrogen system has wide application range and can meet the requirements of various fields; and the nitrogen generator and the liquid nitrogen generator can be directly obtained from the market, and have the advantages of small volume and high efficiency.

Preferably, the sterilizing device is used for supplying gas and/or liquid to the first pipeline to sterilize the inside of the first pipeline; the inner wall of the first pipeline is sterilized and disinfected by introducing gas or liquid into the first pipeline, so that the positive cells of the part left in the delivered nitrogen are adhered to the inner wall of the first pipeline, the sterilization device provides gas or liquid to kill the positive cells of the inner wall of the first pipeline continuously, and the nitrogen is ensured to be in an aseptic and non-toxic condition after being liquefied.

Preferably, the sterilization device is a hydrogen peroxide generator for supplying hydrogen peroxide gas to the interior of the first pipeline; in the middle of producing hydrogen peroxide gas and letting in first pipeline through the hydrogen peroxide generator, hydrogen peroxide gas has strong oxidizing property, and its cell wall that can oxidize positive cell causes positive cell death to realize the effect of disinfecting, its bactericidal effect is good, and hydrogen peroxide gas is pollution-free, and the feature of environmental protection is high.

Preferably, the degerming device comprises a lower tank body, a filter element detachably connected with the lower tank body and an upper tank body sleeved on the filter element, wherein the upper tank body is detachably connected with the lower tank body.

Preferably, the lower tank body is provided with an air inlet pipeline and an air outlet pipeline, and the air inlet pipeline is provided with a sealing element which is in sealing fit with the inner wall of the first pipeline; through set up the sealing member on the intake pipe to contact between this sealing member and the pipeline inner wall, the displacement can not take place for the sealing member this moment, and it has ensured the leakproofness of sealing member between to intake pipe and first pipeline.

Preferably, the sealing element is provided with an inclined wall which is inclined from top to bottom; the side wall of the sealing element is set to be the inclined wall, the whole area of the sealing element is large, when the air inlet pipe is connected with the first pipeline, the contact area of the sealing element is large, the sealing effect is good, nitrogen leakage can be effectively prevented, and external bacteria can be completely prevented from entering the first pipeline from the connection position of the air inlet pipe and the first pipeline.

Preferably, a plurality of sealing convex ribs with C-shaped sections are arranged on the sealing element, and the sealing convex ribs are abutted with the inner wall of the first pipeline; through setting into C shape structure with sealed protruding muscle, it is when inserting the front portion of intake pipe in the middle of the first pipeline, sealed protruding muscle can take place deformation and expand, and it has ensured to be in the state that contacts all the time between sealed protruding muscle and the first pipeline inner wall, and when it had improved the leakproofness, the life that still secret seal was.

Preferably, the air outlet pipe is provided with an ultraviolet disinfection device for irradiating nitrogen by ultraviolet rays; through setting up ultraviolet ray degassing unit can carry out the secondary disinfection to nitrogen gas, it has further strengthened nitrogen gas and is in aseptic nontoxic state.

Preferably, the ultraviolet disinfection device comprises a lamp holder and a plurality of ultraviolet lamp tubes arranged on the lamp holder, and the lamp holder is abutted against the inner wall of the air outlet tube; through setting up lamp stand and a plurality of ultraviolet tube, it ensures that ultraviolet tube carries out even irradiation to nitrogen gas, therefore bactericidal effect is good.

In conclusion, the liquid nitrogen generated by the utility model is sterile and nontoxic, and when the cells are frozen and cultured, the cells are not polluted, so that the survival rate of the cells is guaranteed; and the sterile liquid nitrogen has wide application range and can meet the requirements of various fields.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic structural diagram of the sterilization apparatus of the present invention.

Fig. 3 is an exploded view of the bacteria removing device of the present invention.

Fig. 4 is a schematic structural diagram of the sealing member of the present invention.

Fig. 5 is a partial cross-sectional view of the sealing member of the present invention.

Fig. 6 is a schematic structural view of the ultraviolet disinfection device of the present invention.

Detailed Description

In order to make the technical field personnel understand the utility model discloses the scheme, will combine the drawing in the embodiment of the utility model below, to the technical scheme in the embodiment of the utility model carries out clear, complete description.

As shown in fig. 1-6, an aseptic liquid nitrogen system comprises a nitrogen gas generating device 1, a sterilizing device 2 and a liquid nitrogen generating device 3, wherein the nitrogen gas generating device 1 is an existing nitrogen gas generator in the market, which can be purchased directly, and the nitrogen gas generating device 1 generates nitrogen gas; the sterilizing device 2 is used for filtering and sterilizing the nitrogen generated by the nitrogen generating device 1, and the nitrogen generated by the nitrogen generating device 1 is conveyed to the sterilizing device 2 through the first pipeline 5, namely, the sterilizing device 2 is used for sterilizing and filtering the nitrogen conveyed by the first pipeline 5; wherein the first pipeline 5 is a metal pipe; the nitrogen gas sterilized by the sterilizing device 2 is conveyed to a liquid nitrogen generator 3 through a second pipeline 6 to be compressed into sterile liquid nitrogen, wherein the liquid nitrogen generator 3 is an existing liquid nitrogen generator in the current market and is the prior art; the second pipeline 6 is a metal pipe.

In order to ensure that positive cells are not left on the inner wall of the first pipeline 5 when the nitrogen gas passes through the first pipeline 5, the positive cells are cells except cells needing to be cultured, the sterile liquid nitrogen system also adopts a sterilization device 7 to introduce gas into the first pipeline 5 to kill bacteria on the inner wall of the first pipeline 5; in other embodiments, the inner wall of the first pipeline 5 can be sterilized by introducing liquid; specifically, the sterilization device 7 is a hydrogen peroxide generator, which is an existing hydrogen peroxide generator in the existing market, and is the prior art, and therefore, detailed description thereof is omitted; the gas outlet of the hydrogen peroxide generator corresponds to the branch pipes, and the generated hydrogen peroxide gas is conveyed to the first pipeline 5 through the branch pipes to sterilize the inner wall of the first pipeline 5.

Further, the sterilization device 2 comprises a lower tank body 21, an upper tank body 22 and a filter element, wherein the lower tank body 21 is a metal tank, the filter element is a filter element in an existing sterilization filter in the current market, and the filter element is detachably connected with the lower tank body 21; the upper tank body 22 is a metal tank, the upper tank body 22 is sleeved on the filter element, and the upper tank body 22 is detachably connected with the lower tank body 21.

Specifically, an air inlet pipe 25 and an air outlet pipe 26 are arranged on the lower tank body 21, wherein the air inlet pipe 25 and the air outlet pipe 26 are both metal pipes, nitrogen is blown in through the air inlet pipe 25, and then is blown out through the air outlet pipe 26 after passing through a filter element; a sealing element 27 is sleeved on the end part of the air inlet pipe 25, the sealing element 27 is a rubber ring, and when the air inlet pipe 25 is connected with the first pipeline 5, the sealing element 27 is abutted against the inner wall of the first pipeline 5; the end face of the sealing element 27 is inclined, and the inner wall of the front end of the first pipeline 5 is an inclined plane; a plurality of circles of sealing convex ribs 29 are arranged on the sealing element 27, the sealing convex ribs 29 are rubber rings with C-shaped sections, and when the air inlet pipe is inserted into the first pipeline, the sealing convex ribs 29 are unfolded to be in contact with the inner wall of the first pipeline 5; meanwhile, the first pipeline 5 and the air inlet pipe 25 are connected through a bolt connecting flange.

Further, an ultraviolet disinfection device 8 is arranged in the air outlet pipe 26, and nitrogen is disinfected and sterilized by ultraviolet rays emitted by the ultraviolet disinfection device 8; specifically, the ultraviolet disinfection device 8 comprises a lamp holder 81 and a plurality of ultraviolet lamp tubes 82, the lamp holder 81 is a metal holder, and when the lamp holder 81 is placed in the air outlet tube 26, the outer wall of the lamp holder 81 is abutted against the inner wall of the air outlet tube 26; the ultraviolet lamp 82 is an ultraviolet tube, and the ultraviolet lamp 82 is placed on the lamp holder 81; meanwhile, the control circuit board and the switch of the lamp tube are positioned outside the air outlet pipe 26.

It is obvious that the described embodiments are only some of the embodiments of the present invention, and not all of them. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts shall belong to the protection scope of the present invention.

Claims (9)

1. A sterile liquid nitrogen system, comprising: comprises that

A nitrogen generating device (1) for generating nitrogen;

the sterilizing device (2) is matched with the nitrogen generating device (1) through a first pipeline (5) and is used for sterilizing the nitrogen conveyed by the first pipeline (5);

and the liquid nitrogen generating device (3) is matched with the sterilizing device (2) through a second pipeline (6) and is used for compressing the sterilized nitrogen to form sterile liquid nitrogen.

2. A sterile liquid nitrogen system according to claim 1, wherein: further comprising sterilization means (7) for supplying gas and/or liquid to the first line (5) for sterilizing the interior of the first line (5).

3. A sterile liquid nitrogen system as claimed in claim 2, wherein: the sterilization device (7) is a hydrogen peroxide generator for supplying hydrogen peroxide gas to the interior of the first pipeline (5).

4. A sterile liquid nitrogen system according to claim 1, wherein: degerming device (2) including down jar body (21), with down jar body (21) can dismantle the filter core of connection and overlap and locate last jar body (22) on the filter core, go up jar body (22) with down jar body (21) can dismantle the connection.

5. The sterile liquid nitrogen system of claim 4, wherein: be equipped with intake pipe (25) and outlet duct (26) on lower jar body (21), be equipped with on intake pipe (25) with sealing member (27) of first pipeline (5) inner wall seal fit.

6. The sterile liquid nitrogen system of claim 5, wherein: the sealing element (27) is provided with an inclined wall (28) which is inclined from top to bottom.

7. The sterile liquid nitrogen system of claim 6, wherein: the sealing element (27) is provided with a plurality of sealing convex ribs (29) with C-shaped sections, and the sealing convex ribs (29) are abutted to the inner wall of the first pipeline (5).

8. The sterile liquid nitrogen system of claim 5, wherein: an ultraviolet ray disinfection device (8) used for ultraviolet ray to irradiate nitrogen is arranged on the air outlet pipe (26).

9. A sterile liquid nitrogen system according to claim 8, wherein: the ultraviolet disinfection device (8) comprises a lamp holder (81) and a plurality of ultraviolet lamp tubes (82) arranged on the lamp holder (81), and the lamp holder (81) is abutted to the inner wall of the air outlet pipe (26).

Images