CN211043419U

CN211043419U - Fluorescence immunoassay appearance

Info

Publication number: CN211043419U
Application number: CN201921403770.5U
Authority: CN
Inventors: 邹武; 樊晨慧; 王立威; 杨振; 刘建源
Original assignee: Relia Biotechnologyjiangsu Co ltd
Current assignee: Relia Biotechnologyjiangsu Co ltd
Priority date: 2019-08-27
Filing date: 2019-08-27
Publication date: 2020-07-17
Anticipated expiration: 2029-08-27

Abstract

The utility model discloses a fluorescence immunoassay appearance, including sample storage rack, the test paper box group, the scanning rifle, pipettor tip head storage rack, dehumidification chamber, incubation unit, be used for detecting optical detection module, three-dimensional arm and the frame of reading to the test paper box of the cartridge clip structure that has the radio frequency card, sample storage rack, pipettor tip head storage rack, incubation unit, dehumidification chamber, optical detection module set up respectively in the frame, test paper box group sets up on the dehumidification chamber, three-dimensional arm sets up in the top of frame. Has the advantages that: the full-automatic detection device can be used for fully automatically detecting the antigen to be detected in human serum, plasma, whole blood and urine, and is convenient to operate, efficient and rapid.

Description

Fluorescence immunoassay appearance

Technical Field

The invention belongs to the field of in-vitro diagnostic instruments, and particularly relates to a fluorescence immunoassay analyzer.

Background

At present, in the existing detector, there are usually only 1-3 detection channels or inlets, a large number of patients need to be detected in a large hospital, the number of detection items is large, the traditional detector can detect at most 3 items, the detection is continuously calculated according to 8-hour system, the detection is carried out for 150-160 persons at most one day, the detection time is long, manual operation is needed in the detection process, the labor is wasted, the efficiency is not high, and in addition, part 1 is required according to the safety requirements of GB 4793.1-2007' measuring, controlling and laboratory electrical equipment; general requirements, GB/T14710-: the main performance and technical indexes of the detector are formulated as follows according to the requirements of standards such as special requirements of In Vitro Diagnosis (IVD) medical equipment and the like and the clinical practical use requirements: 1) linear range: and 6 test boxes are used for testing, namely the standard test boxes 1# -6#, and the linear correlation coefficient r is more than or equal to 0.95.

2) Resolution requirements: the resolution of the analyzer was < 20 fM. 3) Repeatability: in a qualified analyzer, the coefficient of variation CV of repeated measurements should not exceed 5%. 4) The accuracy is as follows: the CRP reference substance with three concentrations is adopted for detection, and the relative deviation of the detection result is not more than +/-15.0%.

Aiming at the requirements, how to provide a detection device which can carry out full-automatic detection, convenient operation, high efficiency and rapidness on the antigen to be detected in human serum, plasma, whole blood and urine becomes the technical problem which needs to be solved urgently at present, and can fill the domestic blank.

Disclosure of Invention

The invention aims to overcome the problems in the background technology and provides a fluorescence immunoassay analyzer, which is realized by the following technical scheme:

the fluorescence immunoassay analyzer comprises a sample storage rack, a test paper box group with a cartridge structure of a radio frequency card, a scanning gun, a pipettor tip head storage rack, a dehumidification cavity, an incubation unit, an optical detection module for detecting and reading the test paper box, a three-dimensional mechanical arm and a rack, wherein the sample storage rack, the pipettor tip head storage rack, the incubation unit, the dehumidification cavity and the optical detection module are respectively arranged on the rack, the test paper box group is arranged on the dehumidification cavity, and the three-dimensional mechanical arm is arranged at the top of the rack.

The fluorescence immunoassay appearance's further design lies in, test paper box group has 3 groups of cartridge clips, and 3 cartridge clips export the test paper box simultaneously, and 20 test paper boxes are deposited and are equipped with the air flue that is used for passing through dry gas to every cartridge clip.

The fluorescence immunoassay analyzer is further designed to further comprise a test paper box conveying device, wherein the test paper box conveying device comprises an x-axis track, a ferry block connected to the x-axis track in a sliding mode and a shifting piece arranged on the ferry block, and the shifting piece is driven by an electromagnet to move in the direction perpendicular to the x axis.

The fluorescence immunoassay analyzer is further designed in that the three-dimensional mechanical arm comprises: the X-axis horizontal movement mechanism comprises two parallel guide rails and an X-axis synchronous belt, and each guide rail is provided with a corresponding guide rail sliding seat; the y-axis horizontal movement mechanism comprises a sliding support, a y-axis synchronous belt, a sliding rail, an x-axis sliding seat and a y-axis sliding seat, the y-axis synchronous belt is arranged on one end face of the sliding support, the y-axis sliding seat can be connected to the y-axis synchronous belt in a sliding manner along the sliding rail in a transmission manner, and the x-axis sliding seat is arranged on the other end face of the sliding support and is in transmission connection with the x-axis synchronous belt; elevating system includes synchronous pulley group, y axle slide, lead screw, vertical guide rail, trace move liquid pump, fixed bolster, lifting support, step motor and contains the elevator of screw hole, the fixed bolster is fixed in on the y axle slide, the lead screw rotationally vertical support in on the fixed bolster, step motor sets up on the fixed bolster, the lead screw pass through synchronous pulley group transmission connect in the output shaft of motor, the elevator passes through screw hole connects with the lead screw soon, and the trace moves the liquid pump and passes through lifting support and connect in the elevator.

The fluorescence immunoassay analyzer is further designed in that the lifting mechanism further comprises a vertical sliding rail, the vertical sliding rail is vertically arranged, the upper end and the lower end of the vertical sliding rail are respectively and correspondingly connected with the y-axis sliding seat and the fixed support, and the vertical sliding rail is of a cubic structure.

The fluorescence immunoassay analyzer is further designed in that the lifting block is a rectangular block; the lifting support is composed of an installation surface, a positioning surface and a pump connection surface, wherein the positioning surface is orthogonal to the installation surface, the lifting support is connected with the lifting block through the installation surface, the positioning surface is parallel to and attached to the end surface of one side, corresponding to the lifting block and the vertical sliding rail, of the lifting block, and the lifting block slides along the screw rod under the restraint of the positioning surface.

The fluorescence immunoassay analyzer is further designed in that the positions set in the x-axis horizontal movement mechanism, the y-axis horizontal movement mechanism and the lifting mechanism are all provided with optical sensors.

The fluorescence immunoassay analyzer is further designed in that the incubation unit is a three-piece peltier strip, provided with 16 resting incubation sites.

The fluorescence immunoassay appearance's further design lies in, frame one side that the sample storage rack corresponds is equipped with the scanning rifle, and the sample storage rack adopts the sheet metal construction, and the flux is 48 test tubes, is equipped with the bar code on the test tube.

The utility model has the advantages that:

the utility model discloses a fluorescence immunoassay appearance can carry out full automated inspection to waiting to detect antigen in human serum, plasma, whole blood and the urine, convenient operation, high-efficient swift.

Drawings

FIG. 1 is a schematic view of a fluorescence immunoassay analyzer.

Fig. 2 is a schematic structural diagram of a three-dimensional robot arm device.

Fig. 3 is a schematic structural diagram of the x-axis horizontal movement mechanism.

Fig. 4 is a schematic structural diagram of the y-axis horizontal movement mechanism.

Fig. 5 is a schematic structural diagram of the lifting mechanism.

FIG. 6 is a schematic diagram of the structure of the incubation unit.

Fig. 7 is a schematic structural view of the test paper box conveying device.

Detailed Description

The invention is described in detail below with reference to the drawings and two specific embodiments.

The fluorescence immunoassay analyzer of the embodiment mainly comprises a sample storage rack 7, a test paper box group 5 with a cartridge structure of a radio frequency card, a pipette tip head storage rack 8, a dehumidification cavity 6, an incubation unit 15, an optical detection module for detecting and reading the test paper box, a three-dimensional mechanical arm and a rack 10. The sample storage rack 7, the pipette tip storage rack 8, the incubation unit 12, the dehumidification cavity 6 and the optical detection module are respectively arranged on the frame 7. The test paper box group 5 is arranged on the dehumidifying cavity 6, and the dehumidifying cavity of the embodiment is a cubic cavity. The three-dimensional robot arm is disposed on the top of the frame 10.

The test paper box group of this embodiment has 3 groups of cartridge clips, and 3 cartridge clips export the test paper box simultaneously, and 20 test paper boxes are deposited and are equipped with the air flue that is used for passing through dry gas to every cartridge clip, and every cartridge clip is equipped with the radio frequency card that includes corresponding test item information.

As in FIG. 6, the incubation unit 15 is a three-piece Peltier 151, provided with 16 resting incubation sites.

As shown in fig. 7, the fluorescence immunoassay analyzer of the present embodiment further includes a test paper cassette conveying device 16. The test paper box conveying device 16 mainly comprises an x-axis track 161, a ferry block 162 slidably connected to the x-axis track, and a shifting piece (not shown in the figure) arranged on the ferry block. The ferry block 162 is used for receiving the test paper box of the test paper box group 5. The paddle is driven by an electromagnet 163 to move in a direction perpendicular to the x-axis for transporting the test paper cassettes on the ferry block 162 to the incubation position in the incubation unit 15.

Referring to fig. 2, the three-dimensional arm device adopted in this embodiment mainly includes an x-axis horizontal movement mechanism 1, a y-axis horizontal movement mechanism 2, and a lifting mechanism 3.

As shown in fig. 3, the x-axis horizontal movement mechanism 1 of the present embodiment is mainly composed of two parallel guide rails 11 and an x-axis synchronous belt 12. Each guide rail 11 is provided with a guide rail slide 13, and the guide rail slide 13 can slide along the guide rail 11.

The y-axis horizontal movement mechanism 2 of the present embodiment is mainly composed of a sliding bracket 21, a y-axis synchronous belt 22, a sliding rail 23, an x-axis sliding base 25 and a y-axis sliding base 24. The y-axis timing belt 22 is disposed on one end surface of the slide holder 21 having a rectangular plate shape, and the slide rail 23 is disposed on the corresponding end surface on one side of the y-axis timing belt 22 in parallel with the distribution direction of the y-axis timing belt 22. The y-axis slide 24 is slidably drivingly connected to the y-axis timing belt 22 along the slide rail 23. The x-axis slide carriage 25 is arranged on the other end face of the slide bracket 21 and is in transmission connection with the x-axis synchronous belt 12. The guide rail sliding seat 13 is detachably connected to the end face of the sliding support 21 corresponding to one side of the x-axis sliding seat 25, so that the sliding support is guided by two linear guide rails when sliding in the x-axis direction, and the moving stability of the whole mechanism is improved.

As shown in fig. 4, the lifting mechanism 3 of the present embodiment mainly includes a synchronous pulley set 31, a y-axis slide 32, a screw 33, a vertical guide 34, a micro-displacement pump 35, a fixed bracket 36, a lifting bracket 30, a stepping motor 37, and a lifting block 38 having a threaded hole. The fixed bracket 36 is connected to the y-axis carriage 24. The screw 33 is rotatably supported vertically on the fixing bracket 36. The stepping motor 37 is provided on the fixed bracket 31. The screw 33 is in transmission connection with an output shaft of a stepping motor 37 through a synchronous pulley set 31. The lifting block 38 is screwed with the screw rod 33 through a threaded hole. The micropipette pump 35 is connected to the lift block 38 via the lift bracket 30. On the other hand, the lifting mechanism realizes simple force feedback control by monitoring the change of the current of the stepping motor 37.

In order to ensure the stability of the micro-pipetting pump 35 during the lifting process, a vertical slide rail 34 is additionally arranged on the lifting mechanism 3, the vertical slide rail 34 is vertically arranged, and the upper end and the lower end of the vertical slide rail 34 are respectively and correspondingly connected with the y-axis slide carriage 32 and the fixed bracket 36. The vertical slide rail 34 of the present embodiment is of a cubic structure.

As shown in fig. 5, the lifting block 38 used in the present embodiment is a rectangular block. Correspondingly, the lifting bracket 30 consists of a mounting surface 302 and a positioning surface 301 orthogonal to the mounting surface, and the lifting bracket 30 is fixedly connected with the lifting block 38 through the mounting surface 302. The positioning surface 301 is parallel to the end surfaces of the corresponding sides of the lifting block 38 and the vertical slide rail 34 respectively and is attached to realize that the lifting block slides along the screw rod under the constraint of the positioning surface. The connection of the micropipette pump 35 to the mounting surface 302 provides a folded mounting that saves space. The micro-pipetting pump 35 collects samples, drives the samples to test paper in the test paper box by the three-dimensional mechanical arm, and conveys the results to a peripheral control system of the analyzer after the samples are incubated and the degrees are detected by the optical detection module.

The synchronous belt wheel set and the screw rod transmission two-stage motion form the embodiment, so that the rigidity of the screw rod transmission is fully utilized, and the motion speed of the shaft is ensured through the synchronous belt transmission.

In this embodiment, a light sensor is provided on the vertical slide 34 at a set position in the path of travel relative to the positioning surface, so that when the positioning surface is moved to the set position, an interrupt trigger signal is sent to the control system to indicate that the micropipette pump has reached the set height (y-axis position).

The mounting surface of the three-dimensional mechanical arm device is provided with the screw hole, and the lifting mechanism can be locked by penetrating and connecting the screw in the screw, so that the whole three-dimensional mechanical arm device is fixed.

In the embodiment, the x-axis horizontal motion mechanism and the y-axis horizontal motion mechanism are respectively provided with the synchronous belt tensioning unit for adjusting the tensioning degree of the synchronous belt, so that the tensioning degree of the synchronous belt is relatively consistent when each machine leaves a factory.

The x-axis horizontal movement mechanism and the y-axis horizontal movement mechanism are also provided with optical sensors at set positions of the supports in the mechanism, and in-place interrupt signals are sent out when the corresponding sliding seats run to the set positions.

The sample storage rack of this embodiment adopts the sheet metal construction, the flux IS 48 test tubes, be equipped with the bar code on the test tube, the analysis appearance passes through built-in scanning gun discernment bar code, from user L IS (L IS full name L organisational Information management system, be a set of laboratory Information management system specially designed for hospital clinical laboratory, can constitute the network with laboratory apparatus and computer, make the complicated operation processes such as patient's sample login, experimental data access, report audit, printing distribution, experimental data statistical analysis realize intellectuality, automation and standardized management) and obtain Information such as corresponding detection project.

The fluorescence immunoassay analyzer of the embodiment can carry out full-automatic detection on the antigen to be detected in human serum, plasma, whole blood and urine, and is convenient to operate, efficient and rapid.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.

Claims

1. The fluorescence immunoassay analyzer is characterized by comprising a sample storage rack, a test paper box group with a cartridge structure of a radio frequency card, a scanning gun, a pipettor tip head storage rack, a dehumidification cavity, an incubation unit, an optical detection module for detecting and reading the test paper box, a three-dimensional mechanical arm and a rack, wherein the sample storage rack, the pipettor tip head storage rack, the incubation unit, the dehumidification cavity and the optical detection module are respectively arranged on the rack, the test paper box group is arranged on the dehumidification cavity, and the three-dimensional mechanical arm is arranged at the top of the rack.

2. The fluoroimmunoassay analyzer of claim 1, wherein the strip magazine has 3 sets of cartridges, 3 cartridges being simultaneously discharged from the test strip magazine, each cartridge storing 20 test strip magazines and being provided with an air passage for passing a dry gas.

3. The fluoroimmunoassay analyzer of claim 2, further comprising a test paper box conveying device, wherein the test paper box conveying device comprises an x-axis track, a ferry block slidably connected to the x-axis track, and a shifting piece arranged on the ferry block, and the shifting piece is driven by an electromagnet to move along a direction perpendicular to the x-axis.

4. The fluoroimmunoassay analyzer of claim 1, wherein the three-dimensional robotic arm comprises: the X-axis horizontal movement mechanism comprises two parallel guide rails and an X-axis synchronous belt, and each guide rail is provided with a corresponding guide rail sliding seat; the y-axis horizontal movement mechanism comprises a sliding support, a y-axis synchronous belt, a sliding rail, an x-axis sliding seat and a y-axis sliding seat, the y-axis synchronous belt is arranged on one end face of the sliding support, the y-axis sliding seat can be connected to the y-axis synchronous belt in a sliding manner along the sliding rail in a transmission manner, and the x-axis sliding seat is arranged on the other end face of the sliding support and is in transmission connection with the x-axis synchronous belt; elevating system includes synchronous pulley group, y axle slide, lead screw, vertical guide rail, trace move liquid pump, fixed bolster, lifting support, step motor and contains the elevator of screw hole, the fixed bolster is fixed in on the y axle slide, the lead screw rotationally vertical support in on the fixed bolster, step motor sets up on the fixed bolster, the lead screw pass through synchronous pulley group transmission connect in the output shaft of motor, the elevator passes through screw hole connects with the lead screw soon, and the trace moves the liquid pump and passes through lifting support and connect in the elevator.

5. The fluoroimmunoassay analyzer of claim 4, wherein the lifting mechanism further comprises a vertical slide rail, the vertical slide rail is vertically disposed, the upper and lower ends of the vertical slide rail are correspondingly connected to the y-axis slide carriage and the fixed bracket, respectively, and the vertical slide rail is of a cubic structure.

6. The fluoroimmunoassay analyzer of claim 4, wherein the elevating block is a rectangular block; the lifting support is composed of an installation surface, a positioning surface and a pump connection surface, wherein the positioning surface is orthogonal to the installation surface, the lifting support is connected with the lifting block through the installation surface, the positioning surface is parallel to and attached to the end surface of one side, corresponding to the lifting block and the vertical sliding rail, of the lifting block, and the lifting block slides along the screw rod under the restraint of the positioning surface.

7. The fluoroimmunoassay analyzer of claim 4, wherein the x-axis horizontal movement mechanism, the y-axis horizontal movement mechanism and the elevating mechanism are provided with optical sensors at predetermined positions.

8. The fluoroimmunoassay analyzer of claim 1, wherein the incubation unit is a three-piece peltier strip provided with 16 resting incubation positions.

9. The fluorescence immunoassay analyzer of claim 1, wherein a scanning gun is disposed on one side of the rack corresponding to the sample storage rack, the sample storage rack is of a sheet metal structure, the flux is 48 test tubes, and bar codes are disposed on the test tubes.