CN210982468U - Siphon type liquid-discharging washing container and immunoblotting instrument - Google Patents
Siphon type liquid-discharging washing container and immunoblotting instrument Download PDFInfo
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- CN210982468U CN210982468U CN201921764344.4U CN201921764344U CN210982468U CN 210982468 U CN210982468 U CN 210982468U CN 201921764344 U CN201921764344 U CN 201921764344U CN 210982468 U CN210982468 U CN 210982468U
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Abstract
The utility model provides a washing container and immunity trace appearance of hydrocone type flowing back. The washing container comprises a main tank body, a first siphon branch and a second siphon branch. After washing is finished, liquid is continuously added into the main tank body only by the aid of the liquid adding mechanism or the sample membrane clamping module extends into the deeper depth of the main tank body to improve the liquid level and promote siphon liquid drainage conditions; utilize the siphon principle, can be with the washing container in the whole evacuation of waste liquid to the waste liquid collecting vat, need not to rely on special imbibition pump or control valve, can retrench the structure of relevant professional equipment, reduced manufacturing cost, prevent the weeping, improve the laboratory cleanliness.
Description
Technical Field
The utility model relates to a biological assay auxiliary equipment, especially a washing container and immunity trace appearance of hydrocone type flowing back.
Background
In some biological experiments, samples need to be placed in different test solutions for incubation reaction, and in order to improve the reaction effect, after incubation in one test solution is completed, washing needs to be performed in a washing solution for multiple times. For example, in the immunoblotting test, the total proteins of cells or tissues after electrophoretic separation are transferred from the gel to the NC membrane or PVDF membrane as a solid carrier, and then the proteins or polypeptides on the solid carrier are used as antigens to perform immunoreaction with corresponding antibodies, and then the antibodies are reacted with enzyme or isotope-labeled secondary antibodies, and finally the protein components expressed by specific target genes after electrophoretic separation are detected by substrate color development or autoradiography. In the whole immune reaction experiment, the solid phase carrier membrane needs to be subjected to washing, incubation and washing operations of multiple test solutions and multiple times.
In the cleaning operation, the liquid needs to be changed for many times for washing, and the current means for discharging the washing waste liquid is to install a liquid discharge pipe on a washing container to be connected to a liquid discharge pump, and then install an electromagnetic valve on the liquid discharge pipe to control the on-off of the liquid discharge pipe. The liquid discharge mode is suitable for the conditions of large size, less washing samples and less washing containers. And to in the immunoblotting experiment, appearance membrane size is not big, and appearance membrane sample is many, need dispose the small-size washing container of a large amount in immunoblotting appearance, if every washing container all disposes positive displacement pump and solenoid valve, must cause the pipeline to arrange in a jumble, and manufacturing cost is high, easily causes the weeping moreover, influences the device cleanliness.
SUMMERY OF THE UTILITY MODEL
In order to overcome the defects of the prior art, the utility model aims to provide a siphon-type liquid-discharging washing container and an immunity tracing instrument.
In order to achieve the above object, the technical solution of the present invention is: a siphon type liquid drainage washing container comprises a main groove body, a first siphon branch and a second siphon branch, wherein the bottom of the main groove body is communicated with the bottom of the first siphon branch through a first water through hole, the upper part of the first siphon branch is communicated with the upper part of the second siphon branch through a second water through hole, and the height of a lower water outlet of the second siphon branch is lower than that of the first water through hole; during washing, the liquid level of the washing liquid in the main tank body is lower than the height of the lower edge of the second water passing hole; when flowing back, make earlier the internal liquid level of main tank surpass the top edge height in the water hole is crossed to the second, afterwards the internal liquid of main tank is crossed the water hole and is passed through first siphon branch road and the discharge of second siphon branch road in order from the first of main tank body bottom.
The utility model discloses compare in prior art, after the washing is accomplished, utilize the siphon principle, can with the washing container in the whole evacuation of waste liquid to the waste liquid collecting vat, need not to rely on special imbibition pump or control valve, can retrench the structure of relevant professional equipment, reduced manufacturing cost, prevent the weeping, improve the laboratory cleanliness.
Further, main cell body, first siphon branch road and second siphon branch road formula structure as an organic whole, first siphon branch road and second siphon branch road are adjacent to be set up side by side in the side of main cell body.
Adopt above-mentioned preferred scheme, the length direction of first siphon branch road and second siphon branch road sets up along the length direction of main cell body, adopts the integral type structure, has retrencied the structure, makes things convenient for washing container's installation.
An immunoblotter comprising:
the slot block is provided with a plurality of rows of slots for inserting and matching the reagent box and the washing container;
the sample membrane clamping module is used for clamping a sample membrane to be reacted;
the first moving mechanism is used for driving the sample membrane clamping module to move between the reagent kit and the washing container on the slot block;
a liquid adding mechanism for adding a washing liquid to the washing container.
Adopt above-mentioned technical scheme, according to the immunoblotting experiment demand, insert on the slot piece and join in marriage corresponding kit and washing container, the centre gripping of appearance membrane is on appearance membrane centre gripping module, and first moving mechanism drives the appearance membrane and moves between kit and washing container according to the experimental procedure, adds the washing liquid through liquid feeding mechanism toward washing container in, and after the single washing was accomplished, through hydrocone type discharge washing waste liquid, waste liquid discharge back, rethread liquid feeding mechanism adds the washing liquid in order to wait next washing action. The sample membrane is movable by adopting hanging and clamping, can be fully immersed in the reaction test solution, can fully react, and can effectively save the use amount of the test solution. Adopt the washing container of above-mentioned hydrocone type flowing back, only need continue to add liquid or stretch into the deeper degree of washing container with the help of appearance membrane clamping module with the help of liquid feeding mechanism to promote the liquid level, promote the emergence of siphon flowing back condition, it is more convenient to drain liquid.
Further, the sample membrane clamping module comprises a suspension plate, a support piece, a pressing piece and a locking element, the sample membrane is attached to the support piece, the top end of the sample membrane is clamped between the pressing piece and the support piece, and the support piece and the pressing piece are installed on the suspension plate through the locking element.
Further, the first moving mechanism comprises a front-back translation mechanism and an up-down lifting mechanism, the suspension plate of the sample membrane clamping module is mounted on the moving block of the front-back translation mechanism, and the base of the front-back translation mechanism is mounted on the moving block of the up-down lifting mechanism.
Further, the first moving mechanism further comprises a left-right translation mechanism, and a base of the up-down lifting mechanism is mounted on a moving block of the left-right translation mechanism.
By adopting the preferable scheme, the stable transfer and the oscillating and uniform mixing of the sample membrane between each kit and the washing container can be realized.
Further, liquid feeding mechanism includes that the washing liquid stores bucket, liquid feeding pump, liquid feeding head and second moving mechanism, the washing liquid is stored and is linked together through the pipeline between bucket, liquid feeding pump and the liquid feeding head, the liquid feeding pump is injected into the washing container with the washing liquid in the washing liquid storage bucket through the liquid feeding head, second moving mechanism can drive the removal of liquid feeding head.
By adopting the preferable scheme, when a plurality of washing containers are inserted and matched on the same row of slots on the slot block, the same liquid adding head can be driven to move through the second moving mechanism, and liquid is added into each washing container according to the requirement.
Further, a waste liquid collecting tank is arranged below the slot inserting block, and a lower water outlet of a second siphon branch on the washing container is right opposite to the waste liquid collecting tank.
Adopt above-mentioned preferred scheme, a plurality of washing containers share a waste liquid collecting vat, make things convenient for the waste liquid to collect the processing.
A method for draining waste liquid in a washing container comprises the following steps: firstly, adding a washing liquid into a main tank body of a washing container, moving a sample membrane into the main tank body for washing, wherein the liquid level of the washing liquid in the main tank body is lower than the height of the lower edge of the second water passing hole during washing; when the washing is finished and liquid needs to be drained, a certain amount of liquid is continuously added into the main tank body, so that the liquid level in the main tank body exceeds the height of the upper edge of the second water passing hole, and the liquid in the main tank body is drained from the first water passing hole in the bottom of the main tank body through the first siphon branch and the second siphon branch in sequence.
Another method of draining the waste liquid in the washing vessel: firstly, adding a washing liquid into a main tank body of a washing container through a liquid adding mechanism, clamping a sample membrane on a sample membrane clamping module, driving a supporting sheet of the sample membrane clamping module and the sample membrane into the washing container through a first moving mechanism for washing, and during washing, enabling the liquid level of the washing liquid in the main tank body of the washing container to be lower than the height of the lower edge of a second water through hole; when needing the flowing back after the washing is accomplished, the support piece that drives a kind membrane centre gripping module through first moving mechanism continues to move down in the washing container with a kind membrane to make the interior liquid level of main tank body surpass the upper edge height in second water hole of crossing in the washing container, the interior liquid of main tank body is from the first water hole of crossing of main tank body bottom in order through first siphon branch road and second siphon branch road discharge promptly.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic view of a washing container according to the present invention;
FIG. 2 is a top view of the wash vessel;
FIG. 3 is a cross-sectional view taken along line A-A of FIG. 2;
FIG. 4 is a cross-sectional view taken along line B-B of FIG. 2;
FIG. 5 is a cross-sectional view taken along line C-C of FIG. 2;
FIG. 6 is a schematic structural view of the immunoblotting apparatus of the present invention;
FIG. 7 is a schematic diagram of the structure of one embodiment of a membrane-holding module;
FIG. 8 is a schematic structural view of another embodiment of the membrane-holding module.
Names of corresponding parts represented by numerals and letters in the drawings:
10-a washing vessel; 101-a main trough body; 102-a first siphon branch; 103-a second siphon branch; 104-a first water through hole; 105-a second water through hole; 106-lower water outlet; 20-a slot block; 30-a membrane clamping module; 301-a suspension plate; 302-a supporting sheet; 303-tabletting; 304-a locking element; 40-a first movement mechanism; 401-a front-to-back translation mechanism; 402-an up-down lifting mechanism; 50-a liquid adding mechanism; 501-liquid adding head; 502-a second movement mechanism; 60-a waste liquid collecting tank; 70-kit.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
As shown in fig. 1 to 5, an embodiment of the present invention is: a siphon type liquid drainage washing container comprises a main tank body 101, a first siphon branch 102 and a second siphon branch 103, wherein the bottom of the main tank body 101 is communicated with the bottom of the first siphon branch 102 through a first water through hole 104, the upper part of the first siphon branch 102 is communicated with the upper part of the second siphon branch 103 through a second water through hole 105, and the height of a lower water outlet 106 of the second siphon branch is lower than that of the first water through hole 104; during washing, the liquid level of the washing liquid in the main tank body 101 is lower than the height of the lower edge of the second water through hole 105; when liquid is drained, the liquid level in the main tank body 101 is enabled to exceed the height of the upper edge of the second water through hole 105, and then the liquid in the main tank body is drained from the first water through hole 104 at the bottom of the main tank body through the first siphon branch 102 and the second siphon branch 103 in sequence.
The beneficial effect of adopting above-mentioned technical scheme is: after the washing is accomplished, utilize the siphon principle, can empty the waste liquid in the washing container to the waste liquid collecting vat in, need not to rely on special imbibition pump or control valve, can retrench the structure of relevant professional equipment, reduced manufacturing cost, prevent the weeping, improve the laboratory cleanliness.
In other embodiments of the present invention, the main tank body 101, the first siphon branch 102 and the second siphon branch 103 are of an integral structure, and the first siphon branch 102 and the second siphon branch 103 are adjacently arranged on the side of the main tank body 101. The first siphon branch 102 and the second siphon branch 103 are disposed along the longitudinal direction of the main tank 101. The beneficial effect of adopting above-mentioned technical scheme is: and the structure is simplified by adopting an integrated structure, so that the washing container is convenient to install.
As shown in fig. 6, an immunoblotter comprising:
a plurality of rows of slots for inserting and matching the reagent kit 70 and the washing container 10 are arranged on the slot block 20;
a sample membrane holding module 30 for holding a sample membrane to be reacted;
a first moving mechanism 40 for driving the sample membrane holding module to move between the reagent kit 70 and the washing container 10 on the slot block;
a dosing mechanism 50 for adding a wash liquor to the wash vessel 10.
Adopt above-mentioned technical scheme, according to the immunoblotting experiment demand, insert on the slot piece and join in marriage corresponding kit and washing container, the centre gripping of appearance membrane is on appearance membrane centre gripping module, and first moving mechanism drives the appearance membrane and moves between kit and washing container according to the experimental procedure, adds the washing liquid through liquid feeding mechanism toward washing container in, and after the single washing was accomplished, through hydrocone type discharge washing waste liquid, waste liquid discharge back, rethread liquid feeding mechanism adds the washing liquid in order to wait next washing action. The sample membrane is movable by adopting hanging and clamping, can be fully immersed in the reaction test solution, can fully react, and can effectively save the use amount of the test solution. Adopt the washing container of above-mentioned hydrocone type flowing back, only need continue to add liquid or stretch into the deeper degree of washing container with the help of appearance membrane clamping module with the help of liquid feeding mechanism to promote the liquid level, promote the emergence of siphon flowing back condition, it is more convenient to drain liquid.
As shown in fig. 7, in other embodiments of the present invention, the sample membrane holding module 30 includes a hanging plate 301, a supporting plate 302, a pressing plate 303 and a locking element 304, the sample membrane is attached to the supporting plate 302, the top end of the sample membrane is held between the pressing plate 303 and the supporting plate 302, and the supporting plate 302 and the pressing plate 303 are mounted on the hanging plate 301 through the locking element 304. A plurality of mounting positions for mounting the supporting sheet can be arranged on the suspension plate 301, so that a plurality of groups of experiments can be carried out simultaneously.
As shown in fig. 6, in other embodiments of the present invention, the first moving mechanism 40 includes a front-rear translation mechanism 401 and an up-down lifting mechanism 402, the suspension plate 301 of the sample membrane holding module 30 is mounted on the moving block of the front-rear translation mechanism 401, and the base of the front-rear translation mechanism 401 is mounted on the moving block of the up-down lifting mechanism 402. The specific implementation forms of the front-back translation mechanism 401 and the up-down lifting mechanism 402 are not limited, and can be selected from the prior art, such as a lead screw pair matching guide rail mode, a transmission belt matching linear guide rail mode, a gear rack mode and the like. In order to improve the stability, the front-back translation mechanism 401 and the up-down lifting mechanism 402 may adopt a moving mechanism with a guide rail matched with a screw pair. Can realize the stable transfer and the oscillation mixing of the sample membrane between each reagent kit and the washing container.
The utility model discloses an in other embodiments, appearance membrane clamping module can adopt the chuck form, the first moving mechanism of immunity trace plotter still includes left right translation mechanism, elevating system's base is installed on left right translation mechanism's movable block, the chuck is installed on front and back translation mechanism's movable block, as shown in FIG. 8, every appearance membrane adopts a set of support piece 302, preforming 303 and locking element 304 to carry out the centre gripping, appearance membrane and support piece are placed in the reagent box of reaction during the reaction, when needing to remove, adopt the chuck to press from both sides to get support piece 302 and change appearance membrane reaction position.
As shown in fig. 6, in other embodiments of the utility model, liquid feeding mechanism 50 includes that the washing liquid stores the bucket (not shown in the figure), the liquid feeding pump (not shown in the figure), liquid feeding head 501 and second moving mechanism 502, the bucket is stored to the washing liquid, through the pipeline intercommunication between liquid feeding pump and the liquid feeding head, the washing liquid of liquid feeding pump in with the washing liquid storage bucket pours into washing container 10 into through liquid feeding head 501 into, second moving mechanism 502 can drive liquid feeding head 501 and remove, for reasonable layout structure, second moving mechanism 502 sets up the below at slot piece 20 place platform, second moving mechanism adopts the motor, gear train and driving belt's transmission mode, liquid feeding head is connected with driving belt, the motor removes about driving belt drives the liquid feeding head. When a plurality of washing containers are inserted and matched on the same row of slots on the slot block, the same liquid adding head can be driven to move through the second moving mechanism, and liquid is added into each washing container according to the requirement.
As shown in fig. 6, in other embodiments of the present invention, the waste liquid collecting groove 60 is disposed below the slot block 20, and the lower water outlet 106 of the second siphon branch on the washing container 10 is opposite to the waste liquid collecting groove 60. A plurality of washing containers share a waste liquid collecting tank, and waste liquid collection and treatment are facilitated.
The following describes the operation of an embodiment of the immunoblot analyzer, in conjunction with the flow of immunoblot experiments with a biotin-labeled secondary antibody.
Immunoblotting experimental scheme for biotin-labeled secondary antibody:
the operation and action process of the clamping type immunoblot analyzer is as follows:
1. adding sufficient No. 1 liquid 1 × TBST washing liquid into a washing liquid storage barrel in a liquid adding mechanism;
2. clamping the sample membrane to a sample membrane clamping module;
3. the front and the back groove positions of the inserting groove block are respectively inserted with a reagent box containing No. 2-6 liquid, and the groove position below the liquid adding head is inserted with a washing container;
4. and the immunoblotting instrument drives the sample membrane to perform incubation reaction and washing in each kit and washing container in sequence according to a preset program until the reaction is finished.
In other embodiments of the present invention, a liquid draining method is used for draining waste washing liquid in a washing container, wherein a washing liquid is first added into a main tank body of the washing container, a sample membrane is moved into the main tank body for washing, and during washing, the liquid level of the washing liquid in the main tank body is lower than the height of the lower edge of a second water through hole; when the washing is finished and liquid needs to be drained, a certain amount of liquid is continuously added into the main tank body, so that the liquid level in the main tank body exceeds the height of the upper edge of the second water passing hole, and the liquid in the main tank body is drained from the first water passing hole in the bottom of the main tank body through the first siphon branch and the second siphon branch in sequence.
In other embodiments of the present invention, a liquid draining method is used for draining a washing waste liquid in a washing container, firstly adding a washing liquid into a main tank body of the washing container through a liquid adding mechanism, clamping a sample membrane on a sample membrane clamping module, driving a supporting sheet of the sample membrane clamping module and the sample membrane into the washing container through a first moving mechanism for washing, wherein during washing, the liquid level of the washing liquid in the main tank body of the washing container is lower than the height of the lower edge of a second water passing hole; when needing the flowing back after the washing is accomplished, the support piece that drives a kind membrane centre gripping module through first moving mechanism continues to move down in the washing container with a kind membrane to make the interior liquid level of main tank body surpass the upper edge height in second water hole of crossing in the washing container, the interior liquid of main tank body is from the first water hole of crossing of main tank body bottom in order through first siphon branch road and second siphon branch road discharge promptly.
The above embodiments are only for illustrating the technical conception and the features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and the protection scope of the present invention can not be limited thereby, and all equivalent changes or modifications made according to the spirit of the present invention should be covered in the protection scope of the present invention.
Claims (8)
1. A siphon type liquid drainage washing container is characterized by comprising a main tank body, a first siphon branch and a second siphon branch, wherein the bottom of the main tank body is communicated with the bottom of the first siphon branch through a first water through hole, the upper part of the first siphon branch is communicated with the upper part of the second siphon branch through a second water through hole, and the lower water outlet of the second siphon branch is lower than the first water through hole; during washing, the liquid level of the washing liquid in the main tank body is lower than the height of the lower edge of the second water passing hole; when flowing back, make earlier the internal liquid level of main tank surpass the top edge height in the water hole is crossed to the second, afterwards the internal liquid of main tank is crossed the water hole and is passed through first siphon branch road and the discharge of second siphon branch road in order from the first of main tank body bottom.
2. The siphonic draining washing container according to claim 1, wherein the main tank body, the first siphon branch and the second siphon branch are of an integrated structure, and the first siphon branch and the second siphon branch are adjacently arranged on the side surface of the main tank body side by side.
3. An immunoblotter, comprising:
a slot block, wherein a plurality of rows of slots for inserting and matching the reagent kit and the washing container with the siphon type drainage of claim 1 or 2 are arranged on the block body;
the sample membrane clamping module is used for clamping a sample membrane to be reacted;
the first moving mechanism is used for driving the sample membrane clamping module to move between the reagent kit and the washing container on the slot block;
a liquid adding mechanism for adding a washing liquid to the washing container.
4. The immunoblotter of claim 3 wherein the membrane-like holding module comprises a hanging plate, a support plate, a pressing plate and a locking element, the membrane-like body is attached to the support plate, the top end of the membrane-like body is held between the pressing plate and the support plate, and the support plate and the pressing plate are mounted on the hanging plate by the locking element.
5. The immunoblotting instrument of claim 4 wherein said first moving mechanism comprises a forward and backward translation mechanism and an up and down lifting mechanism, said sample membrane holding module suspension plate is mounted on the moving block of the forward and backward translation mechanism, and said forward and backward translation mechanism base is mounted on the moving block of the up and down lifting mechanism.
6. The immunoblotter of claim 5 wherein the first movement mechanism further comprises a side-to-side translation mechanism, the base of the up-down lift mechanism being mounted on the moving block of the side-to-side translation mechanism.
7. The immunoblotting instrument of claim 3, wherein the liquid feeding mechanism comprises a washing liquid storage barrel, a liquid feeding pump, a liquid feeding head and a second moving mechanism, the washing liquid storage barrel, the liquid feeding pump and the liquid feeding head are communicated through a pipeline, the liquid feeding pump injects the washing liquid in the washing liquid storage barrel into the washing container through the liquid feeding head, and the second moving mechanism can drive the liquid feeding head to move.
8. The immunoblotting instrument of claim 7 wherein a waste collection tank is positioned below said slot block, and a lower outlet of a second siphon branch on said washing container is opposite to said waste collection tank.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921764344.4U CN210982468U (en) | 2019-10-21 | 2019-10-21 | Siphon type liquid-discharging washing container and immunoblotting instrument |
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| CN201921764344.4U CN210982468U (en) | 2019-10-21 | 2019-10-21 | Siphon type liquid-discharging washing container and immunoblotting instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112239033A (en) * | 2020-09-29 | 2021-01-19 | 李晓娟 | Experimental waste liquid treatment device for disease control center |
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2019
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112239033A (en) * | 2020-09-29 | 2021-01-19 | 李晓娟 | Experimental waste liquid treatment device for disease control center |
| CN112239033B (en) * | 2020-09-29 | 2022-05-06 | 李晓娟 | Experimental waste liquid treatment device for disease control center |
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