CN210808588U

CN210808588U - Rice double-drum drought-resisting identification device based on deep and shallow root stress treatment

Info

Publication number: CN210808588U
Application number: CN201921514089.8U
Authority: CN
Inventors: 楼巧君; 吴国平; 陈亮
Original assignee: SHANGHAI AGROBIOLOGICAL GENE CENTER
Current assignee: SHANGHAI AGROBIOLOGICAL GENE CENTER
Priority date: 2019-09-11
Filing date: 2019-09-11
Publication date: 2020-06-23
Anticipated expiration: 2029-09-11

Abstract

The utility model belongs to the technical field of crops resistance appraisal, a rice binocular drought resisting appraisal device based on deep and shallow root coercion is handled is disclosed, including cultivation device and cultivation sleeve, and cultivation device arranges in and cultivates sleeve directly over and the center pin coincidence, still includes box and control valve. The device is used for sowing, sprouting, sand filling, transplanting, cultivating and processing, respectively controlling the growth environment of the inner root system and the outer root system of the culture sleeve to carry out stress processing according to the seedling stage, the tillering stage, the flowering stage or the grouting stage of rice, and regularly observing and recording growth data and the root amount of the inner cylinder and the outer cylinder. The utility model discloses a telescopic simple structure and unique, the deep root is whole to fall into the inner tube, and shallow root is whole to fall into in the urceolus, and the deep shallow root of analysis biological function separately can provide technical support for the block-based research root system function.

Description

Rice double-drum drought-resisting identification device based on deep and shallow root stress treatment

Technical Field

The utility model belongs to the technical field of the crops resistance appraisal, concretely relates to rice binocular drought resisting appraisal device based on deep shallow root is coerced respectively and is handled, stresses respectively through deep root to rice and shallow root and realizes the research root system of blocking.

Background

The plant root system has special morphological characteristics such as density, depth, range and the like of root system distribution, and the characteristics have great effect on stress resistance of plants, particularly drought resistance stress, so that the development of root system structure research and the refinement of the influence of the root stress at different positions on the plants have important practical significance. However, the prior cultivation and observation means can not respectively process and investigate deep roots and shallow roots, and similar technical reports are not found in documents and patents.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a rice binocular drought resisting identification device based on the root is coerced respectively in depth layer is handled, to the different angle depth layer roots of rice coerce respectively handle the cultivation and special design obtains for rice root system structural research especially refines the influence that different positions root coerces to rice growth.

The above object of the utility model is realized through following technical scheme:

a rice double-drum drought resisting identification device based on deep and shallow root respective stress treatment comprises:

the cultivation device is of a rice basket type structure with a round flat bottom and uniform holes on the surface;

the cultivation sleeve consists of an outer cylinder and an inner cylinder, the outer cylinder and the inner cylinder are mutually isolated, the bottom of the outer cylinder is provided with a water through port I, the inner diameter of the inner cylinder is the same as the diameter of the round flat bottom of the cultivation device, and the inner diameter of the outer cylinder is the same as the diameter of the upper edge of the cultivation device; wherein:

the cultivation device is arranged right above the cultivation sleeve, the round flat bottom of the cultivation device is tightly contacted with the opening of the inner cylinder, and the central axes of the outer cylinder, the inner cylinder and the cultivation device are kept coincident; the height of the cultivation device is smaller than the height difference between the inner cylinder and the outer cylinder;

further comprising: the box body is used for placing the culture sleeve, and the control valve is used for controlling the water inlet I and the water outlet II to input and output water/or nutrient solution.

Furthermore, the bottom area of the box body is larger than that of the culture sleeve, a plurality of culture cylinders can be placed, the height of the box body is not lower than that of the water through port, and the water level can be set according to the treatment requirement.

Further, the height of the cultivation device is at least 3cm smaller than the height difference between the inner cylinder and the outer cylinder.

Furthermore, the water through opening II is located on the side wall of the bottom of the inner barrel, a PVC pipe is connected to extend through the wall of the outer barrel, the water through opening II is isolated from the outer barrel, and the PVC pipe is connected with a hose.

Furthermore, the cultivation device is made of plastic or stainless steel materials.

Further, the control valve is a clamp.

The utility model has the advantages that: the double-cylinder drought-resistant identification device for rice is simple and unique in structure, the culture sleeve is placed in a large box body through the sleeve type structural design, and the water or nutrient solution level in the box body is controlled, so that the moisture and nutrient components of culture mediums of the inner cylinder and the outer cylinder can be independently and accurately controlled without influencing each other, and the consistency of culture conditions of different plants is ensured. The design through interior outer tube makes the deep root of plant all fall into the inner tube, and the shallow root is all fallen into in the urceolus, can be more simple and convenient carry out the coerce processing respectively to the deep and shallow root of plant, can provide technical support for the regional research root system function through the respective biological function of analysis deep and shallow root.

Drawings

FIG. 1 is a front view of a double-barreled drought resistance identification device for rice; wherein: 1-a cultivation device; 2-culture sleeve, 201-outer cylinder, 202-inner cylinder, 203-water inlet I, 204-water inlet II.

FIG. 2 is a plan view of a rice binocular drought resistance testing apparatus.

FIG. 3 shows rice seedlings grown for 25 days.

FIG. 4 shows rice seedlings being subjected to drought treatment.

FIG. 5 shows IRAT109 rice plants drought-treated for 35 days; wherein: the left 3 strains were T1 and the right 3 strains were T2.

FIG. 6 is a photograph of a root washing process.

FIG. 7 is a photograph of washed roots; wherein: the left side is Minghui 63-T3, and the right side is IRAT 109-T3.

Detailed Description

The present invention will be further explained with reference to the drawings and examples.

Referring to fig. 1 and 2, the double-drum drought resistance identification device for rice based on deep and shallow root stress treatment comprises:

the cultivation device 1 is of a rice basket type structure with a round flat bottom and uniform holes on the surface;

the cultivation sleeve 2 consists of an outer cylinder 201 with a water through port I203 at the bottom and an inner cylinder 202 with a water through port II 204 at the bottom which are mutually isolated, the diameter of the round flat bottom of the cultivation device 1 is the same as the inner diameter of the inner cylinder 202, the diameter of the upper edge of the cultivation device is the same as the inner diameter of the outer cylinder 201, and the height of the cultivation device is smaller than the height difference between the inner cylinder 202 and the outer cylinder 201;

the culture device also comprises a box body for placing the culture sleeve 2 and a control valve for controlling the water inlet I203 and the water outlet II 204 to input and output water/nutrient solution; wherein:

the cultivation device 1 is placed right above the cultivation sleeve 2, and the round flat bottom thereof is in contact with the opening of the inner cylinder 202, so that the central axes of the outer cylinder 201, the inner cylinder 202 and the cultivation device 1 are kept coincident.

In one embodiment, the bottom area of the case is larger than that of the culture sleeve 2, and three culture cartridges can be placed.

In one embodiment, the height of the cultivation apparatus 1 is at least 3cm smaller than the height difference between the inner cylinder 202 and the outer cylinder 201.

In one embodiment, the water passage II 204 is located on the bottom side wall of the inner barrel 202, and is connected with a PVC pipe extending through the wall of the outer barrel 201, the water passage II 204 is isolated from the outer barrel 201, and the PVC pipe is connected with a hose.

The method for carrying out the drought resistance identification on the rice by using the double-drum drought resistance identification device for the rice comprises the following specific steps:

(1) seeding and Germination

The plump seeds of the Minghui 63 and the IRAT109 are selected, soaked in clear water for one day, germinated under the condition of 28 ℃ plus illumination for 12 hours, and can be prepared for transplantation after 10 days.

(2) Sand irrigation and transplantation

Fine sand with uniform screening particles is filled in the culture sleeve shown in figure 2 and reaches the upper edge of the inner cylinder. The bottom surface of the plastic flat-bottomed basket with the small holes is flatly placed on the inner cylinder. The inner diameter of the upper edge of the basket is the same as that of the outer cylinder, the diameter of the bottom of the basket is the same as that of the inner cylinder, and the height of the basket is at least 3cm smaller than the height difference of the inner cylinder and the outer cylinder.

And adding sand after watering, and finally, enabling the height of the sand to be flush with the upper edge of the basket.

Selecting strong and tidy seedlings, transplanting the seedlings to the center of a basket, and sowing the seedlings with the depth about 2 cm below the surface. After transplanting, the seedlings normally grow for 25 days, and the conditions of the seedlings are shown in figure 3.

(3) Cultivation and treatment

The plants are cultivated under the temperature and illumination environment required by the normal growth of the plants, and nutrient solution or fertilizer is added periodically. The experiment starts water cut-off treatment from 35 days after transplantation, and the period is the most sensitive period of rice drought, namely the booting period.

4 treatments were set: CK (water in both inner and outer cylinders), T1 (no water in inner cylinder and no water in outer cylinder), T2 (no water in inner cylinder and no water in outer cylinder) and T3 (no water in both inner and outer cylinders). When the water supply is cut off, the clamp on the hose is opened, and the water in the cylinder needing drought is naturally drained through the hose. Meanwhile, the barrel is placed in a large box body, water with a certain depth is added in the box, clamps on 203 and/or 204 outer hoses corresponding to the inner barrel and/or the outer barrel to be watered are opened, the hoses are immersed in the water, the barrel to be watered and the box body outside are connected through the hoses, the water level of the barrel to be watered and the water level of the box body outside are kept consistent, and the water level heights of all the box bodies are kept consistent and are controlled to be 10 cm.

(4) Observing and recording

Data such as plant height, tillering, leaf rolling, moisture content, biological yield, etc. were regularly observed and recorded as shown in table 1.

Table 1: phenotypic data after 3 drought treatments for two rice varieties

Fig. 4 and 5 are photographs of the drought treatment process. After the treatment, the agronomic characteristics such as root quantity of the inner cylinder and the outer cylinder can be respectively investigated by a root washing method, and the root washing process and the results are shown in fig. 6 and 7.

As shown in table 1, comparing the different materials, the deep root ratio and drought resistance of the rice variety IRAT109 were significantly greater than those of the bright recovered 63 (table 1 and fig. 7) regardless of the treatments, and the bright recovered 63 was more susceptible to drought, and when T3 was completely drought, it was not possible to shoot ears and the setting percentage was zero. IRAT109 can maintain a high level of fruit set when the deep root or the shallow root is drought alone. Comparing the different treatments of the same material, the fruit set rate was higher for the T2 treatment than for both the T1 and T3 treatments, indicating that drum water, i.e. deep root water, is more important for plant yield development. The response difference of deep and shallow roots to drought is large, samples need to be further expanded for research, the method successfully shows the difference influence of the drought stress of roots at different positions of different materials on crops, and the method has good application value in plant root system research.

Claims

1. A double-drum drought-resistant identification device for rice based on deep and shallow root stress treatment is characterized in that,

the method comprises the following steps: the cultivation device is of a rice basket type structure with a round flat bottom and uniform surface with small holes;

the cultivation sleeve consists of an outer cylinder and an inner cylinder, the outer cylinder and the inner cylinder are mutually isolated, the bottom of the outer cylinder is provided with a water through port I, the inner diameter of the inner cylinder is the same as the diameter of the round flat bottom of the cultivation device, and the inner diameter of the outer cylinder is the same as the diameter of the upper edge of the cultivation device; wherein: the cultivation device is arranged right above the cultivation sleeve, the round flat bottom of the cultivation device is tightly contacted with the upper opening of the inner cylinder, the central axes of the outer cylinder, the inner cylinder and the cultivation device are kept coincident, and the height of the cultivation device is smaller than the height difference between the inner cylinder and the outer cylinder;

2. The binocular drought resistance assessment device for rice according to claim 1,

the diameter and the depth of the outer cylinder are both larger than those of the inner cylinder, the outer cylinder and the inner cylinder are isolated from each other, and the circle centers of the bottoms of the outer cylinder and the inner cylinder are overlapped.

3. The binocular drought resistance assessment device for rice according to claim 1,

the height of the cultivation device is at least 3cm smaller than the height difference between the inner cylinder and the outer cylinder.

4. The binocular drought resistance assessment device for rice according to claim 1,

the water through opening II is positioned on the bottom side wall of the inner barrel and is isolated from the outer barrel;

the water through port II is connected with a PVC pipe which extends through the outer cylinder wall to be connected with a hose.

5. The binocular drought resistance assessment device for rice according to claim 1,

the cultivation device is made of plastic or stainless steel materials.

6. The binocular drought resistance assessment device for rice according to claim 1,

the control valve is a clamp.