CN210742392U - System for adjusting power frequency magnetic field intensity according to animal cell biological indexes - Google Patents
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Abstract
The utility model provides a system for adjust power frequency magnetic field intensity according to animal cell biology index, include: the device comprises a power frequency magnetic field generating device, a magnetic field intensity measuring device, an animal neuroma blast test box and a processor; the magnetic field intensity measuring device is connected with the power frequency magnetic field generating device; the animal neuroma blast test box is arranged in the power frequency magnetic field generating device and is used for exposing the animal neuroma blast in the animal neuroma blast test box to the magnetic field generated by the power frequency magnetic field generating device; the processor is connected with the magnetic field intensity measuring device and the power frequency magnetic field generating device, and is used for receiving magnetic field intensity data sent by the magnetic field intensity measuring device, controlling the power frequency magnetic field generating device to generate a magnetic field with preset intensity according to biological index data of the animal neuroma blast in the magnetic field measured in real time, and determining the magnetic field intensity corresponding to the maximum value of the biological index data as the optimal magnetic field intensity.
Description
Technical Field
The utility model relates to a power frequency magnetic field technical field particularly, relates to a system for adjust power frequency magnetic field intensity according to animal cell biology index.
Background
An Extremely low frequency electromagnetic field (ELF EMF) refers to an alternating electromagnetic field with a frequency of 300Hz or less, and is generally generated by transmission and transformation engineering and various household appliances in daily life. In recent years, research finds that ELF EMF exposure shows good influence and excellent application prospect in the biological field. Meanwhile, in order to avoid the damage effect of overexposure of a large dose of ELF EMF on organisms, the definition of the exposure intensity threshold of the ELF EMF has important scientific significance and application value, however, no corresponding system for adjusting the exposure intensity of the extremely-low-frequency electromagnetic field exists in the prior art.
Disclosure of Invention
In view of this, the utility model provides a system for adjust power frequency magnetic field intensity according to animal cell biological index aims at solving among the prior art problem that the extremely low frequency electromagnetic field exposes intensity and is difficult to the accurate regulation.
The utility model provides a system for adjust power frequency magnetic field intensity according to animal cell biology index, include: the device comprises a power frequency magnetic field generating device, a magnetic field intensity measuring device, an animal neuroma blast test box and a processor; the magnetic field intensity measuring device is connected with the power frequency magnetic field generating device and used for detecting the magnetic field intensity generated by the power frequency magnetic field generating device; the animal neuroma blast test box is arranged in the power frequency magnetic field generating device and is used for exposing the animal neuroma blast in the animal neuroma blast test box to the magnetic field generated by the power frequency magnetic field generating device; the processor is connected with the magnetic field intensity measuring device and the power frequency magnetic field generating device and used for receiving magnetic field intensity data sent by the magnetic field intensity measuring device, controlling the power frequency magnetic field generating device to generate a magnetic field with preset intensity according to real-time measured biological index data of the animal neuroma blast in the magnetic field, and determining the magnetic field intensity corresponding to each biological index data when the biological index data reaches the maximum value as the optimal magnetic field intensity.
Further, in the system for adjusting the power frequency magnetic field strength according to the biological index of the animal cell, the power frequency magnetic field generating device includes: an alternating current power supply and a field coil; the output end of the alternating current power supply is connected with the magnetic field coil and used for generating a power frequency magnetic field with adjustable strength around the magnetic field coil.
Furthermore, in the system for adjusting the power frequency magnetic field intensity according to the animal cell biological index, the contour of the magnetic field coil is square, and the side length of the magnetic field coil is greater than or equal to 0.4 m.
Furthermore, in the system for adjusting the power frequency magnetic field intensity according to the animal cell biological index, the magnetic field measuring device is provided with the three-dimensional magnetic field probe for measuring the magnetic field intensity in different areas away from the magnetic field coil.
Furthermore, in the system for adjusting the power frequency magnetic field strength according to the animal cell biological index, the maximum magnetic field strength which can be generated by the power frequency magnetic field generating device is greater than or equal to 2 mT.
Further, in the system for adjusting the power frequency magnetic field strength according to the animal cell biological index, the power frequency magnetic field generating device generates a magnetic field with a preset strength by adjusting the voltage.
Further, in the system for adjusting the power frequency magnetic field strength according to animal cell biological indexes, the biological indexes comprise the content of A β in the supernatant, the phosphorylation level of Tau protein in cells and the oxidative stress level in cells.
Furthermore, in the system for adjusting the power frequency magnetic field strength according to the animal cell biological index, the biological index data is the variation between the animal neuroma blast exposure group and the control group.
The utility model provides a system according to power frequency magnetic field intensity is adjusted to animal cell biology index, produce the magnetic field of predetermineeing intensity through treater control power frequency magnetic field generating device, and confirm optimum magnetic field intensity through the change volume of the biological index data of the animal neuroma mother cell that exposes in this magnetic field, and, it is short to test exposure time through the cell, detection speed is fast, can confirm optimum magnetic field intensity fast, research and development for various medical instrument provides theoretical support and practice and guides, industrially have extensive using value.
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Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the invention. Also, like reference numerals are used to refer to like parts throughout the drawings. In the drawings:
fig. 1 is a schematic structural diagram of a system for adjusting power frequency magnetic field intensity according to animal cell biological indicators, provided by an embodiment of the present invention.
Detailed Description
Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art. It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict. The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
Referring to fig. 1, a system for adjusting power frequency magnetic field intensity according to animal cell biological indicators according to an embodiment of the present invention includes: the device comprises a power frequency magnetic field generating device 1, a magnetic field intensity measuring device, an animal neuroma blast test box 2 and a processor; the magnetic field intensity measuring device is connected with the power frequency magnetic field generating device 1 and used for detecting the magnetic field intensity generated by the power frequency magnetic field generating device 1; the animal neuroma blast test box 2 is arranged in the power frequency magnetic field generating device 1 and is used for exposing the animal neuroma blast in the animal neuroma blast test box 2 to the magnetic field generated by the power frequency magnetic field generating device; the processor is connected with the magnetic field intensity measuring device and the power frequency magnetic field generating device 1, and is used for receiving magnetic field intensity data sent by the magnetic field intensity measuring device, controlling the power frequency magnetic field generating device 1 to generate a magnetic field with preset intensity according to biological index data of the animal neuroma blast in the magnetic field measured in real time, and determining the corresponding magnetic field intensity as the optimal magnetic field intensity when the biological index data reach the maximum value.
Specifically, the power frequency magnetic field generating device 1 may be any one of the magnetic field generating devices in the prior art, and may generate a stable power frequency magnetic field. In this embodiment, the maximum magnetic field intensity that the power frequency magnetic field generating device 1 can generate is greater than or equal to 2 mT. It should be noted that, in the embodiment of the present invention, the power frequency magnetic field generating device includes itself and the magnetic field region generated around itself.
More specifically, the power frequency magnetic field generating apparatus 1 may include: an ac power supply 11 and a field coil 12; the output end of the alternating current power supply 11 is connected with the field coil 12, and is used for generating a power frequency magnetic field with adjustable strength around the field coil 12. The contour of the magnetic field coil 12 may be square, circular, or the like, and in the present embodiment, the contour of the magnetic field coil 12 is square, and the side length thereof is 0.4m or more. The magnetic field measuring device is provided with a three-dimensional magnetic field probe used for measuring the magnetic field intensity of different areas of the distance magnetic field coil.
Animal neuroma blast test box 2 can be any kind of test box that can deposit animal cell among the prior art, the utility model discloses do not do any restriction to its shape, size and material. The animal neuroma blast can be a mouse neuroma blast (N2 a), and the exposure time of the mouse neuroma blast to the magnetic field can be determined according to actual conditions, such as 8 hours.
The embodiment of the utility model provides an in, in order to confirm optimum power frequency magnetic field intensity, need test respectively in the magnetic field of multiunit intensity, in every experimental group, including exposing group and contrast group test box, in corresponding every experimental group, expose the animal neuroma mother cell test box in group and the contrast group and can be a plurality of, for example 22, wherein, design 11 as exposing the group, 11 are as contrast group, can use hydrogen peroxide solution (H2 a) in advance with the mouse neuroma mother cell (N2 a) that exposes group and contrast group2O2) Inducing cell oxidative stress or over-expressing tau protein or A β, and incubating to simulate the state in vivo, thereby detecting the initial values of various biological indexes of the mouse neuroma blast.
Since the magnetic field intensity is different in different regions from the magnetic field coil, the plurality of animal neuroma blast test cartridges in each set of intensity test can be placed in a plurality of regions having different distances from the center of the magnetic field coil. For example, when testing, the animal neuroma blast test kit of one of the exposed groups can be placed in the center of the magnetic field coil, and the animal neuroma blast test kits of the other exposed groups can be placed in different areas outside the coil for subsequent testing. The 11 comparison groups are respectively and correspondingly placed at the 11 exposure groups, but the 11 comparison groups are not processed by a power frequency magnetic field, the biological index data of the animal neuroma blast in the 22 test boxes are compared and analyzed, after the local superior magnetic field intensity range is determined, the test boxes are reselected according to smaller step length in the local superior magnetic field intensity range and are respectively placed in different magnetic field regions, the above tests are repeated, and the like are repeated until the optimal power frequency magnetic field intensity is found from the multiple local superior magnetic field intensities.
The biological indexes can comprise the content of A β in supernatant, the phosphorylation level of Tau protein in cells and the oxidation stress level in cells, the biological index data of animal neuroblastoma mother cells are the variation between an animal neuroblastoma mother cell exposed group and a control group, the maximum value of the biological index data is the maximum variation between the animal neuroblastoma mother cell exposed group and the control group, of course, the variation is beneficial variation, namely, the content of A β in supernatant, the phosphorylation level of Tau protein in cells and the reduction of the oxidation stress level in cells in the exposed group and the control group are all the maximum, in practice, each group of experiment can be repeated for many times, the average value of the variation between the exposed group and the control group is taken, and the comparison between the groups is carried out by adopting a statistical variance analysis method, the P value of less than 0.05 is considered to have statistical difference between the two groups, wherein the intervention strength with the maximum variation is the optimal magnetic field strength.
Taking a 500 mu T magnetic field exposure as an example, in animal neuroma blast cells cultured in vitro, an AD cell model is constructed by over-expressing Tau protein, the cells are placed in a 50Hz/500 mu T magnetic field and a 0 mu T control magnetic field, and the cells are exposed for 8 hours, after the exposure is finished, cell protein is collected, and the phosphorylation level of Tau protein in the cells and the expression of kinase esterase (GSK 3 β, PP2AC and CDK 5) at the upstream are detected by a Western blot method.
In practice, can also adopt the system of multiunit regulation power frequency magnetic field intensity to different magnetic field intensity are exposed the experiment to a plurality of animal neuroma blast simultaneously, can further shorten test cycle, with the more swift optimal magnetic field intensity that obtains.
It can be clearly seen from the above that, in the system for adjusting the power frequency magnetic field strength according to the biological index of the animal cell provided in this embodiment, the processor controls the power frequency magnetic field generating device to generate the magnetic field with the preset strength, and determines the optimal magnetic field strength according to the variation of the biological index data of the animal neuroma blast exposed in the magnetic field, so as to provide theoretical support and practical guidance for the research and development of various medical instruments, and have a wide application value in industry.
The embodiment of the utility model provides a use method according to device of animal cell biology index regulation power frequency magnetic field intensity includes following step:
and step S1, placing the animal neuroma blast test box in a power frequency magnetic field generating device, and exposing the animal neuroma blast in the animal neuroma blast test box in a magnetic field with preset intensity for preset time.
Specifically, N magnetic field strengths may be set in advance, where N is an integer of 2 or more; under each preset magnetic field intensity, respectively placing a plurality of animal neuroma blast test boxes in a magnetic field area with preset intensity generated by a power frequency magnetic field generating device for exposure for a preset time, wherein the magnetic field intensity at each position far away from the center of the magnetic field area is reduced in sequence. The magnetic field intensity of different positions far away from the center of the magnetic field area is detected by a magnetic field measuring device, and the animal neuroma blast test boxes are respectively placed at corresponding positions.
During specific implementation, the power supply can be pre-regulated to enable the magnetic field intensity of the central area of the power frequency magnetic field generating device to be a first preset value, the magnetic field intensity of different areas away from the center of the coil is different, the position where the magnetic field intensity is 0 can be found outside the coil of the power frequency generating device through the magnetic field measuring device, a plurality of magnetic field intensity values are found between the first preset value and 0 at preset magnetic field intervals, then a plurality of animal neuroma mother cell test boxes are taken to be placed at the positions of different magnetic field intensities, and the biological indexes of the neuroma mother cells of the animals can be changed after being exposed for a period of time. The exposure time can be determined according to actual conditions, and the biological indexes of the animal neuroblastoma blast can be changed. The exposure time of the animal neuroma blast to the magnetic field can be determined as a practical matter, for example 8 hours. Meanwhile, the same number of animal neuroma blast test boxes are selected as a control group, and the magnetic field intensity of the animal neuroma blast test boxes of the control group is 0.
The animal neuroma blast test kit may be a plurality of samples, for example, 22, wherein 11 are designed as an exposure group and 11 are designed as a control group, and since the magnetic field strength is different from different regions of the magnetic field coil, the plurality of animal neuroma blast test kits may be respectively placed in a plurality of regions having different distances from the center of the magnetic field coil. For example, when testing, the animal neuroma blast test kit of one of the exposed groups can be placed in the center of the magnetic field coil, and the animal neuroma blast test kits of the other exposed groups can be placed in different areas outside the coil for subsequent testing. The 11 samples of the control group are respectively correspondingly placed at the positions of the 11 samples of the exposure group, but the 11 samples of the control group are not processed by the power frequency magnetic field.
And step S2, detecting the biological index of the animal neuroma blast after the preset time of exposure.
Specifically, the biological indicators of the animal neuroblastoma mother cells can comprise the content variation of A β in supernatant, the variation of phosphorylation level of Tau protein in cells and oxidative stress water in cellsThe amount of flat variation. In this example, the test was carried out using mouse neuroma blast (N2 a), and hydrogen peroxide (H2 a) was previously used for mouse neuroma blast (N2 a)2O2) Inducing cell oxidative stress or over-expressing tau protein or A β incubation to simulate the state in vivo, thereby detecting the initial values of various biological indexes of the mouse neuroma blast in the exposure group and the control group.
In this embodiment, the means for detecting each biological indicator of mouse neuroma blast is not limited, and may include, but is not limited to, the following steps:
(1) collecting cell supernatant, and detecting the level of A β in the supernatant by enzyme-linked immunosorbent assay (without detection when incubating by A β);
(2) detecting the level of ROS in the cells by a DC-FHDA probe and using flow cytometry, detecting the apoptosis occurrence rate by Annexin V/PI double staining and adopting flow cytometry;
(3) extracting cell protein, detecting expression of pathway protein related to regulation and control of oxygen free radical generation and elimination in cells and expression of pathway protein related to apoptosis by Western blot, and simultaneously detecting phosphorylation levels of different sites of tau protein in cells and expression of regulation and control of kinase esterase, expression of A β major pathway protein, etc.
And step S3, controlling the power frequency magnetic field generating device to generate a magnetic field which changes in a preset step length, taking another animal neuroma blast test box, and repeating the step 1-2 before finishing the preset N groups of magnetic field exposure tests with the strength, wherein N is an integer more than or equal to 2.
Specifically, the step size in the present embodiment refers to magnetic field strength of several sizes, for example, 200 μ T, 50 μ T, 10 μ T, 1 μ T, and the like. Since the step size is sufficiently small, it can be considered that the magnetic field strength continuously changes. In practice, the magnetic field intensity can be increased by presetting a step length, the magnetic field intensity can also be reduced by presetting a step length, the maximum value and the minimum value of the magnetic field intensity can be preset, and the magnetic field intensity can be adjusted according to the proper step length so as to carry out the magnetic field exposure test on the animal neuroma blast in different animal neuroma blast test boxes. In order to ensure the accuracy of the test result, multiple groups of magnetic field strengths are often selected for exposure tests, each group of exposure tests can correspond to one magnetic field strength value, and multiple test boxes can be selected for the magnetic field exposure tests with each group of strength for testing, so that the result of the magnetic field exposure tests with the specific strength is more accurate. In addition, multiple animal neuroma blast test cartridges may be tested in multiple replicates while varying the magnetic field strength in larger increments of field strength (e.g., 200 μ T).
Step S4, in a preset N groups of magnetic field exposure tests, determining the corresponding magnetic field intensity when the biological index data of the animal neuroma blast reaches the maximum value as the optimal magnetic field intensity.
Specifically, the biological index data of the animal neuroma blast is the variation between the exposed group of the animal neuroma blast and the control group. The control group in the magnetic field exposure test of each set of intensities was the same number of test cartridges as the exposure group except that the control group did not receive the magnetic field exposure. The method comprises the steps that corresponding biological index data results can be obtained in magnetic field exposure tests of each group of strength, local superior magnetic field strength in each group of tests can be determined according to the biological index data, N local superior magnetic field strengths can be determined in N groups of exposure tests, and the optimal magnetic field strength is determined from the N local superior magnetic field strengths, namely the magnetic field strength of the position where a test box where the biological index data of the animal neuroma mother cells reach the maximum value is determined as the optimal magnetic field strength. In practice, each group of experiments can be repeated for a plurality of times, the mean value of the variation between the exposure group and the control group is taken, statistical variance analysis is adopted for comparison between the groups, the P value of less than 0.05 is considered that the two groups have statistical difference, and the intervention strength with the maximum beneficial variation is the optimal power frequency magnetic field strength.
Taking 11 animal neuroma blast boxes in the exposure group as an example, if the preset maximum magnetic field intensity is 2mT and the minimum magnetic field intensity is 0mT, during the test, firstly, the voltage of an alternating current power supply is adjusted to ensure that the magnetic field intensity in the middle area of a magnetic field coil is 2 mT; measuring the magnetic field intensity in different areas away from the center of the magnetic field coil by a magnetic field measuring device, then placing the test box containing the animal neuroma blast in the magnetic field areas with different intensities, as shown in figure 1, placing the 1 st test box in the magnetic field area with the magnetic field intensity of 2mT, placing the 2 nd test box in the magnetic field area with the magnetic field intensity of 1.8mT, placing the 3 rd test box in the magnetic field area with the magnetic field intensity of 1.6mT, … …, until the 11 th test box is placed in the area with the magnetic field intensity close to 0mT, and detecting various biological indexes in the exposure group after continuous exposure for 8 hours; after the animal neuroblastoma test boxes in 11 control groups are placed for 8 hours without being treated by a power frequency magnetic field, the biological indexes of the animal neuroblastoma blast cells in each control group are detected, and the biological index data of the exposed group and the biological index data of the control group are statistically compared. If the comparison shows that under the exposure of the magnetic field strength in a certain interval, various biological indexes have obvious beneficial variable quantity, the magnetic field strength exposure effect in the interval is considered to be better, the magnetic field strength can be changed by changing the voltage of an alternating current power supply, and an exposure test is further carried out at an interval of 50 muT or less, such as 10 muT, so that the optimal magnetic field strength is obtained.
In conclusion, the system for adjusting the power frequency magnetic field strength according to the biological indexes of the animal cells controls the power frequency magnetic field generating device to generate a magnetic field with preset strength through the processor, determines the optimal magnetic field strength through the variation of the biological index data of the animal neuroma blasts exposed in the magnetic field, can quickly determine the optimal magnetic field strength through short exposure time and high detection speed of cell tests, provides theoretical support and practical guidance for the research and development of various medical instruments, and has wide application value in industry; the use method is simple, the operation is easy, and the accuracy of the test result is high.
It will be apparent to those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (7)
1. A system for adjusting power frequency magnetic field strength according to animal cell biological indicators, comprising: the device comprises a power frequency magnetic field generating device, a magnetic field intensity measuring device, an animal neuroma blast test box and a processor; wherein,
the magnetic field intensity measuring device is connected with the power frequency magnetic field generating device and is used for detecting the magnetic field intensity generated by the power frequency magnetic field generating device; the maximum magnetic field intensity which can be generated by the power frequency magnetic field generating device is equal to 2 mT;
the animal neuroma blast test box is arranged in the power frequency magnetic field generating device and is used for exposing the animal neuroma blast in the animal neuroma blast test box to the magnetic field generated by the power frequency magnetic field generating device;
the processor is connected with the magnetic field intensity measuring device and the power frequency magnetic field generating device and used for receiving magnetic field intensity data sent by the magnetic field intensity measuring device, controlling the power frequency magnetic field generating device to generate a magnetic field with preset intensity according to real-time measured biological index data of the animal neuroma blast in the magnetic field, and determining the magnetic field intensity corresponding to each biological index data when the biological index data reaches the maximum value as the optimal magnetic field intensity.
2. The system for regulating power frequency magnetic field intensity according to animal cell biological indicators of claim 1, wherein the power frequency magnetic field generating device comprises: an alternating current power supply and a field coil; wherein,
and the output end of the alternating current power supply is connected with the magnetic field coil and used for generating a power frequency magnetic field with adjustable strength around the magnetic field coil.
3. The system for regulating power frequency magnetic field strength according to animal cell biological indicators of claim 2, wherein the magnetic field coil has a square outline with a side length of 0.4m or more.
4. The system for regulating power frequency magnetic field strength according to animal cell biological indicators of claim 2, wherein the magnetic field strength measuring device is provided with a three-dimensional magnetic field probe for measuring the magnetic field strength in different areas from the magnetic field coil.
5. The system for regulating power frequency magnetic field intensity according to animal cell biological indicators of claim 1, wherein the power frequency magnetic field generator generates a magnetic field with a preset intensity by adjusting voltage.
6. The system for regulating power frequency magnetic field strength according to animal cell biological indicators of claim 1, wherein the biological indicators comprise a content of A β in supernatant, a phosphorylation level of Tau protein in cells, and an oxidative stress level in cells.
7. The system of claim 6, wherein the biological indicator data is the amount of change between the animal neuroma blast exposure group and the control group.
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