CN210736738U - Automatic sample application device for microfluidic gene detection chip - Google Patents
Automatic sample application device for microfluidic gene detection chip Download PDFInfo
- Publication number
- CN210736738U CN210736738U CN201921613171.6U CN201921613171U CN210736738U CN 210736738 U CN210736738 U CN 210736738U CN 201921613171 U CN201921613171 U CN 201921613171U CN 210736738 U CN210736738 U CN 210736738U
- Authority
- CN
- China
- Prior art keywords
- sample application
- array
- gene detection
- spotting
- microfluidic gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 19
- 238000001514 detection method Methods 0.000 title claims abstract description 18
- 239000000523 sample Substances 0.000 claims abstract description 114
- 230000007246 mechanism Effects 0.000 claims abstract description 25
- 230000006978 adaptation Effects 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 3
- 238000002493 microarray Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 238000005842 biochemical reaction Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108020004518 RNA Probes Proteins 0.000 description 1
- 239000003391 RNA probe Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000012775 microarray technology Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- -1 tissues Proteins 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an automatic sample application device for micro-fluidic gene detection chip, its technical scheme is: the sample application device comprises a sample application array, wherein the sample application array is arranged on one side of a lifting mechanism, a sample application table is arranged below the sample application array, and a probe pool corresponding to the sample application array is arranged in the sample application table; the lifting mechanism comprises a stepping motor and a linear sliding table mechanism connected with the stepping motor, and the sample application array is fixedly connected with the linear sliding table mechanism. The utility model discloses a sample application array and sample application platform probe cell can dismantle the replacement to the micro-fluidic runner, reaches the purpose of the different runners of adaptation.
Description
Technical Field
The utility model relates to a bioengineering technical field especially relates to an automatic sample application device for micro-fluidic gene detection chip.
Background
Micro, automatic and high-throughput parallel detection is one of the development trends of biomedical detection technology, and microarray technology is a representative technology reflecting the trend. Microarray chip technology has been applied to the detection of genes, proteins, tissues, polypeptides, sugars, etc. It fixes several biochemical probes on the substrate (glass, nitrocellulose, nylon, etc.) in the form of tiny liquid drops to form micro-array, then makes the whole array and the sample to be tested undergo the related biochemical reaction operation, every liquid drop is an independent reaction system, and the reaction result is presented in the form of fluorescent array. It is essentially a parallel operation of a large number of biochemical reactions to achieve high throughput detection. The spotting instrument is a special equipment for preparing a microarray chip, which disperses a liquid reagent into minute droplets and arranges the droplets on a substrate in an array. The inventor finds that the sample applicator is few in types, most of the sample applicator is expensive, and the sample applicator brings difficulty to small and medium-sized scientific research institutions.
SUMMERY OF THE UTILITY MODEL
In order to overcome the not enough of prior art, the utility model provides an automatic sample application device for micro-fluidic gene detection chip, applicable in the contact sample application of most gene slide, and sample application array and sample application platform probe cell can dismantle the replacement to the micro-fluidic runner, reach the purpose of the different runners of adaptation.
The utility model adopts the following technical proposal:
an automatic sample application device for a microfluidic gene detection chip comprises a sample application array, wherein the sample application array is arranged on one side of a lifting mechanism, a sample application table is arranged below the sample application array, and a probe pool corresponding to the sample application array is arranged in the sample application table; the lifting mechanism comprises a stepping motor and a linear sliding table mechanism connected with the stepping motor, and the sample application array is fixedly connected with the linear sliding table mechanism.
Further, the sample application array is provided with sample application needles distributed in multiple rows and multiple columns, and the sample application needles are detachable.
Further, the sample application table is placed above the base, and a door-shaped support is fixedly arranged above the base; step motor arranges in door type support top perpendicularly, and sharp slip table mechanism is located inside the door type support.
Furthermore, the sample application array is connected with the linear sliding table mechanism through the fixed table, and the sample application array is embedded in the fixed table through the clamping groove.
Further, the linear sliding table mechanism comprises a lead screw connected with the stepping motor, a nut in threaded connection with the lead screw, and a sliding block arranged on the outer side of the nut.
Furthermore, the end part of the lead screw is connected with the door-shaped bracket through a bearing.
Furthermore, the sample application array is connected with an air pump through a connecting pipe.
Compared with the prior art, the beneficial effects of the utility model are that:
(1) the position of the probe pool of the utility model corresponds to the sample application array, and different types of probes can be simultaneously sample applied after different types of sample application needles are arranged in the probe pool; the probe pool and the sample application array can be disassembled and replaced, so that the aim of adapting to different flow channels is fulfilled;
(2) the utility model can realize automatic sample application through the stepping motor and the controller thereof; and after the sample application is finished, the residual probe liquid can be automatically cleaned.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the application and, together with the description, serve to explain the application and are not intended to limit the application.
Fig. 1 is a perspective view of a first embodiment of the present invention;
fig. 2 is a front view of a first embodiment of the present invention;
fig. 3 is a side view of a first embodiment of the present invention;
fig. 4 is a top view of a first embodiment of the present invention;
FIG. 5 is a top view of the sample application region according to the first embodiment of the present invention;
the device comprises a stepping motor 1, a mounting seat 2, a lead screw 3, a lead screw 4, a nut 5, a sample application array 6, a sample application table 7, a base 8, a sliding block 9, a fixing plate 10, a side plate 11, a fixing table 12, a lead 13, a connecting pipe 14, a bearing 15, a sample application needle 16, a probe pool 17, a bottom plate 18 and a top plate.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an", and/or "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof;
for convenience of description, the words "up", "down", "left" and "right" when appearing in this application are intended only to designate directions that are consistent with the up, down, left and right directions of the drawings themselves, and not to limit the structure, but merely to facilitate description of the invention and to simplify description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be considered as limiting the application.
The terms "mounted", "connected", "fixed", and the like in the present application should be understood broadly, and for example, the terms "mounted", "connected", and "fixed" may be fixedly connected, detachably connected, or integrated; the two components can be connected mechanically or electrically, directly or indirectly through an intermediate medium, or connected internally or in an interaction relationship, and specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The first embodiment is as follows:
the present invention will be described in detail with reference to the accompanying drawings 1-5, and specifically, the structure is as follows:
the embodiment discloses an automatic sample application device for a microfluidic gene detection chip, which comprises a sample application array 5, a sample application table 6, a door-shaped support and a lifting mechanism, wherein the lifting mechanism is vertically installed with the door-shaped support, the sample application array 5 is arranged on one side of the door-shaped support and connected with the lifting mechanism, and the sample application table 6 is positioned below the sample application array 5; the lifting mechanism can drive the sample application array 5 to move up and down to realize sample application operation.
Further, the door-shaped bracket comprises a top plate 18, a base 7, a fixing plate 9 and two side plates 10, wherein the fixing plate 9 is connected between the two side plates 10 and forms a structure with one side (the side opposite to the fixing plate 9) opened; the top plate 18 is fixed above the side plate 10 and the fixing plate 9, and the base 7 is arranged at the bottom of the side plate 10 and the fixing plate 9. The side plate 10 is provided with a plurality of slotted holes for observing the height of the sample application array 5.
Elevating system includes step motor 1 and the sharp slip table mechanism that links to each other with step motor 1, spotting array 5 and sharp slip table mechanism fixed connection. Step motor 1 is fixed in the top of door type support roof 18 through mount pad 2, step motor 1 passes through wire 12 external connection driver and controller, realizes automatic programming, realizes the function of automatic rising sample application.
Further, inside sharp slip table mechanism located door type support, it includes lead screw 3, nut 4 and slider 8, 3 one end of lead screw passes through the shaft coupling with step motor 1's motor shaft and links to each other, and the lead screw 3 other end passes through bearing 14 and links to each other with base 7.
The nut 4 is in threaded connection with the lead screw 3, and the lead screw 3 rotates to drive the nut 4 to move up and down along the lead screw; the outside of nut 4 is fixed with slider 8, slider 8 links to each other with sample application array 5 through fixed station 11, and sample application array 5 moves up and down along lead screw 3 along with fixed station 11 together. In this embodiment, the fixed station 11 has been seted up the draw-in groove, and the sample application array 5 inlays in the fixed station 11, conveniently changes sample application array 5.
The sample application array 5 comprises a plurality of sample application needles 15, the sample application needles 15 are distributed in a plurality of rows and columns, the pore size of the sample application needles 15 can be changed according to the diameter of the microfluidic flow channel, and different types of probes can be simultaneously applied with samples after the sample application needles 15 are placed into the probe pool 16. The sample application array 5 is connected with an air pump through a connecting pipe 13, and the function of automatically cleaning residual probes is realized.
The sample application platform 6 comprises a bottom plate 17 and a probe pool 16 arranged above the bottom plate 17, and the probe pool 16 is detachably connected with the bottom plate 17. The probe pool 16 and the sample array 15 can realize the individualized design of the position and the number of the probes according to different flow channels, can be disassembled and replaced, and achieve the purpose of sample application on different substrates.
The working principle of the embodiment is as follows:
after injecting different RNA or mi RNA probe liquid into the sample application pool 16, adjusting the parameters of the stepping motor 1, connecting the stepping motor 1 with a driver and a controller through a lead 12, adjusting the downlink parameters of the controller according to the distance between the sample application array 5 and the probe pool 16, and enabling the sample application needle 15 to be in contact with the probe pool 16 to realize the liquid suction of the sample application needle due to the liquid siphoning effect. And adjusting the uplink parameters of the controller, placing the gene slide in the sample application table 6 during the uplink period of the sample application array 5, and setting the downlink parameters of the controller again to realize contact sample application between the sample application array 5 and the gene slide.
After the sample application is finished, the sample application array 5 is externally connected with an air pump through a connecting pipe 13, and the effect of automatically cleaning residual probe liquid is realized.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims (7)
1. An automatic sample application device for a microfluidic gene detection chip is characterized by comprising a sample application array, wherein the sample application array is arranged on one side of a lifting mechanism, a sample application table is arranged below the sample application array, and a probe pool corresponding to the sample application array is arranged in the sample application table; the lifting mechanism comprises a stepping motor and a linear sliding table mechanism connected with the stepping motor, and the sample application array is fixedly connected with the linear sliding table mechanism.
2. The automated spotting device for microfluidic gene detection chips of claim 1 wherein the spotting array has spotting pins distributed in multiple rows and columns and the spotting pins are detachable.
3. The automatic sample application device for the microfluidic gene detection chip as claimed in claim 1, wherein the sample application table is placed above the base, and a gate-shaped bracket is fixedly installed above the base; step motor arranges in door type support top perpendicularly, and sharp slip table mechanism is located inside the door type support.
4. The automatic sample application device for the microfluidic gene detection chip as claimed in claim 3, wherein the sample application array is connected to the linear sliding table mechanism through a fixing table, and the sample application array is embedded in the fixing table through a clamping groove.
5. The automatic spotting device for microfluidic gene detection chips of claim 4, wherein the linear sliding table mechanism comprises a lead screw connected with a stepper motor, a nut in threaded connection with the lead screw, and a slide block installed outside the nut.
6. The automated spotting device for microfluidic gene detection chips of claim 5 wherein the end of the lead screw is connected to the gantry by a bearing.
7. The automated spotting device for microfluidic gene detection chips of claim 1 wherein the spotting array is connected to an air pump through a connecting tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921613171.6U CN210736738U (en) | 2019-09-25 | 2019-09-25 | Automatic sample application device for microfluidic gene detection chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921613171.6U CN210736738U (en) | 2019-09-25 | 2019-09-25 | Automatic sample application device for microfluidic gene detection chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210736738U true CN210736738U (en) | 2020-06-12 |
Family
ID=70981970
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921613171.6U Expired - Fee Related CN210736738U (en) | 2019-09-25 | 2019-09-25 | Automatic sample application device for microfluidic gene detection chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210736738U (en) |
-
2019
- 2019-09-25 CN CN201921613171.6U patent/CN210736738U/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6589483B1 (en) | Liquid dispenser | |
| US6764648B1 (en) | Robotic microchannel bioanalytical instrument | |
| JP6857671B2 (en) | Weighing head, weighing device with weighing head, and weighing method using weighing head | |
| EP1344050B1 (en) | Apparatus and method for filling and cleaning channels and inlet ports in microchips used for biological analysis | |
| DE60214851T2 (en) | MICROFLUIDIC DEVICE AND METHOD FOR CHEMICAL TRIALS | |
| US7541001B2 (en) | Automatic pipetting and analyzing device | |
| RU2014136713A (en) | DEVICE AND METHOD FOR AUTOMATIC ANALYSIS OF BIOLOGICAL SAMPLES | |
| WO1996042004A2 (en) | Method and system for inhibiting cross-contamination in fluids of combinatorial chemistry device | |
| JP2021505204A (en) | Polymerase chain reaction system | |
| WO2020181825A1 (en) | Nucleic acid extraction apparatus | |
| CN210736738U (en) | Automatic sample application device for microfluidic gene detection chip | |
| CN116496881B (en) | Full-automatic gene processing method | |
| CN112284865A (en) | Slide pretreatment system | |
| WO2017203744A1 (en) | Nucleic acid examination device | |
| US20110132109A1 (en) | Device and process for the formation of microdepositions | |
| CN113234585B (en) | Integrated single-cell sample preparation device and method for performing cell reaction by adopting same | |
| CN215586536U (en) | Liquid suction device for liquid transfer instrument | |
| CN104962454A (en) | Full-automatic nucleic acid molecule hybridization instrument | |
| CN212864734U (en) | Automatic nucleic acid extraction instrument | |
| CN114214185A (en) | Nucleic acid extraction instrument | |
| CN215140044U (en) | Automatic liquid transfer device | |
| US7235163B2 (en) | Loading features for channel array | |
| CN116410843A (en) | Biochip all-in-one | |
| CN210065777U (en) | Sequencer for chromosome detection | |
| JP2015232577A (en) | Analyzer assembly platform |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200612 |