CN210538461U

CN210538461U - Vitrification freezing carries pole

Info

Publication number: CN210538461U
Application number: CN201921014499.6U
Authority: CN
Inventors: 毕方成
Original assignee: Taipingyang Kangtai Scientific Apparatus Jinan Co ltd
Current assignee: Taipingyang Kangtai Scientific Apparatus Jinan Co ltd
Priority date: 2019-06-30
Filing date: 2019-06-30
Publication date: 2020-05-19
Anticipated expiration: 2029-06-30

Abstract

The utility model relates to a pole is carried in vitrification refrigeration, including the body of rod and sleeve pipe, the body of rod is including the pole body, toper pole and the cell slide glass that link up in order, and the one end that toper pole and cell slide glass are connected is the path end. The sleeve can be sleeved outside the cell slide and the conical rod. The cross section of the cell slide is rectangular. One side of the cell slide is provided with a u-shaped groove extending along the length direction of the cell slide, and the u-shaped groove is positioned in the middle of the cell slide in the width direction. By improving the structure of the cell slide, the restriction factor of the cell slide on the survival rate in cell preservation is eliminated, and the survival rate of the frozen cell sample is improved.

Description

Vitrification freezing carries pole

Technical Field

The utility model relates to a cold-stored technical field of cell is particularly a pole is carried in vitrification freezing.

Background

With the continuous development of biotechnology, the application of the method for performing vitrification freezing storage on biological cells is more and more extensive. The prior vitrification freezing storage device mainly comprises a core bar, a cell slide fixedly arranged at one end of the core bar and a sleeve which can be sleeved outside the core bar to seal the cell slide. The cell slide is made of transparent materials and can bear ultralow temperature. In use, biological cells are attached to a section of the cell slide adjacent the free end thereof. In the process of using the existing biological cell preservation rod, when biological cells are attached to a cell slide, if the volume of the cells is relatively large, the cell body part cannot be well infiltrated by the vitrification solution, and is exposed outside the liquid, so that the survival of the cells is adversely affected, and the survival rate of the preservation is low.

SUMMERY OF THE UTILITY MODEL

For the structure that improves the cell slide glass to the restriction degree of survival rate in the cell preservation, improve the survival rate that the cell was frozen and was preserved, the utility model provides a pole is carried in vitrification freezing.

The utility model provides a technical scheme that its technical problem adopted is: the utility model provides a pole is carried in vitrification refrigeration, includes the body of rod and sleeve pipe, and the body of rod is including the pole body, toper pole and the cell slide glass that link up in order, and the one end that toper pole and cell slide glass are connected is the path end. The sleeve can be sleeved outside the cell slide and the conical rod. The cross section of the cell slide is rectangular. One side of the cell slide is provided with a u-shaped groove extending along the length direction of the cell slide, and the u-shaped groove is positioned in the middle of the cell slide in the width direction. Black marks are typically provided at the free end of the cell slide. Further, one end of the u-shaped groove extends to the black mark part and the end of the groove is spaced from the end of the black mark part. One end that u type groove extends to black mark is semicircle flute shape, and the bottom surface of this semicircle flute is the fillet form with the turning between the facade, and the both ends of fillet are tangent with bottom surface and facade respectively, establish the dog-ear department in groove into the fillet structure, can prevent that the cell from pressing from both sides in the dog-ear position that becomes 90 degrees, be not convenient for draw. When the u-shaped groove is arranged, the cells with relatively large volume can still be fully immersed in the vitrification solution, and the adverse effect on the survival rate of the preserved cells caused by the fact that the parts of the cells are exposed out of the liquid is prevented. After the u-shaped groove is arranged on the cell slide, the thickness of the bottom wall and the side wall of the groove can still ensure that the cell slide has enough rigidity on the whole under the condition of relatively thin thickness, and the cell slide can form a supporting function to bear the weight of cells and vitrification solution, and cannot be easily deformed. The end part of the u-shaped groove is set to be in a semicircular groove shape, and the corner between the bottom surface and the vertical surface of the semicircular groove is in a round angle shape, so that convenience is brought to the cell taken out of the u-shaped groove, and meanwhile, the cell is guaranteed to float in the liquid at the semicircular groove end of the u-shaped groove and cannot slide to the outside from the u-shaped groove.

In a specific embodiment of the patent, the other end of the rod body is provided with a tag section, the transverse cross section of which is rectangular in structure, and the free end is provided with an end block. Steps are formed at the joint of the two ends of the label section, the end block and the rod body. When the label is used, the label is adhered and sleeved on the label section and cannot slide to the rod body or slide from one end of the end block when being separated from the label section due to the limitation of the step structures at the two ends.

In the specific implementation manner of this patent, the one end of the nearly pole body of toper pole is formed with the tight structure of cover, this tight structure of cover can with the inner wall of sheathed tube column chamber port matches, makes the body of rod with the sleeve pipe is inserted and is connected integratively, and can break away from each other again when exerting with the axial force that carries on the back mutually.

Specifically, the tightening structure is a conical ring arranged on the outer wall of the conical rod, and the small-diameter end of the conical ring is close to the cell slide.

Specifically, the tightening structure comprises a sleeve ring and a cylindrical rod section formed by the tapered rod and the root of the rod body, and the outer diameter of the cylindrical rod section is smaller than the large outer diameter of the tapered rod. The lantern ring is sleeved on the cylindrical rod section. The outer peripheral surface of the lantern ring, which is far away from one side of the rod body, is a conical surface a, the small-diameter end of the conical surface a faces outwards, and a radial flange is arranged on the other side of the lantern ring. The two ends of the lantern ring are limited by a shaft shoulder formed by the rod body relative to the cylindrical rod section and a shaft shoulder formed by the large outer diameter end of the conical rod relative to the cylindrical rod section, and only can do limited reciprocating sliding motion relative to the cylindrical rod section along the axial direction. A section of conical surface cavity is formed at the end port of the cylindrical cavity of the sleeve. The conical surface a can be inserted into the conical surface cavity, so that the outer conical surface of the conical surface a and the inner conical surface of the conical surface cavity are mutually pressed to connect the sleeve and the sleeve ring together. Preferably, the collar has a radial flange portion with an outer diameter greater than an outer diameter of the stem body.

The utility model has the advantages that: by improving the structure of the cell slide, the restriction factor of the cell slide on the survival rate in cell preservation is eliminated, and the survival rate of the frozen cell sample is improved. In addition, by improving the structure form of the inserting connection between the rod body and the sleeve, the instantaneous vibration action can not occur in the operation process of separating the rod body and the sleeve, so that the separation operation becomes stable and controllable, the possibility of the contact between a cell slide and the inner wall of the sleeve in the separation operation is effectively reduced, namely, the possibility of damage to the cell sample in the operation is reduced, and the survival rate of the frozen cell sample is improved.

Drawings

Fig. 1 is a schematic structural diagram of an overall embodiment of the present patent.

3 fig. 32 3 ( 3 a 3) 3 is 3 a 3 schematic 3 cross 3- 3 sectional 3 structure 3 at 3 a 3- 3 a 3 in 3 fig. 31 3. 3

FIG. 2(b) is a partial axial sectional structure diagram of the cell slide in FIG. 1.

Fig. 3 is a schematic structural diagram of another overall embodiment of the present patent.

Fig. 4 is a partially enlarged structural view at B in fig. 3.

In the figure: 1 rod body, 11 rod bodies, 12 conical rods, 121 conical surface rings, 122 cylindrical rod sections, 13 cell slides, 131 u-shaped grooves, 132 black marks, 14 label sections, 141 end blocks, 2 sleeves, 21 cylindrical cavities, 22 conical cavities and 3 lantern rings

Detailed Description

The drawings in the specification show the structure, ratio, size, etc. only for the purpose of matching with the content disclosed in the specification, so as to be known and read by those skilled in the art, and not for the purpose of limiting the present invention, so the present invention does not have the essential meaning in the art, and any structure modification, ratio relationship change or size adjustment should still fall within the scope covered by the technical content disclosed in the present invention without affecting the function and achievable purpose of the present invention. Meanwhile, the terms "upper", "lower", "front", "rear", "middle", and the like used in the present specification are for the sake of clarity only, and are not intended to limit the scope of the present invention, and changes or adjustments of the relative relationship thereof are also considered to be the scope of the present invention without substantial changes in the technical content.

Fig. 1 to 3 show a vitrification frozen bar, in which fig. 1 is a plan view and fig. 3 is a front view partially in section. This vitrification freezing carries pole includes the body of rod 1 and sleeve pipe 2, and the body of rod 1 is including the pole body 11, toper pole 12 and the cell slide 13 that link up in order, and the conical rod 12 link mutually with cell slide 13 is the path end. The sleeve 2 can be sleeved outside the cell slide 13 and the conical rod 12. One end of the tapered rod 12 close to the rod body 11 is provided with a tightening structure which can be matched with the inner wall of the port of the cylindrical cavity 21 of the sleeve 2, so that the rod body 1 and the sleeve 2 are tightly connected into a whole and can be separated from each other when axial force in the opposite direction is applied.

The cross section of the cell slide 13 is rectangular-like (as shown in fig. 2 (a)). One surface of the cell slide 13 is provided with a u-shaped groove 131 extending along the length direction thereof, and the u-shaped groove 131 is located at the central position in the width direction of the cell slide 13, as shown in fig. 2 (a). Generally black markings 132 are provided at the free end of the cell slide 13. Further, one end of the u-shaped groove 131 extends to the black mark portion and the end of the groove is spaced from the end of the black mark portion, as shown in fig. 2 (b). One end of the u-shaped groove 131 extending to the black mark 132 is shaped like a semicircular groove, a corner between the bottom surface and the vertical surface of the semicircular groove is shaped like a rounded corner, and two ends of the rounded corner are tangent to the bottom surface and the vertical surface respectively, as shown in fig. 1 and fig. 2 (b). The dog-ear of the groove chamber with u type groove is established to the fillet structure, can prevent that the cell from pressing from both sides in 90 degrees dog-ear positions, is not convenient for draw. When the u-shaped groove 131 is arranged, cells with relatively large volume can still be fully immersed in the vitrification solution, and the cells are prevented from being partially exposed out of the solution to cause adverse effects on the survival rate of the preserved cells. After the u-shaped groove 131 is formed on the cell slide, the thickness of the bottom wall and the side wall of the groove can still ensure that the cell slide has enough rigidity as a whole and can form a supporting function to bear the weight of cells and vitrification solution without easy deformation under the condition of relatively thin thickness. The end part of the u-shaped groove 131 is designed to be in a semicircular groove shape, and the corner between the bottom surface and the vertical surface of the semicircular groove is in a round angle shape, so that convenience is brought to taking out cells from the u-shaped groove, and meanwhile, the cells are guaranteed to float in liquid at the semicircular groove end of the u-shaped groove and cannot slide to the outside from the u-shaped groove. When the sleeve is taken off from the rod body, the rod body (namely the cell slide) is subjected to obvious inertial impact if the operation is slightly improper, so that the liquid arranged at the end part of the u-shaped groove is easy to axially move, and the liquid is easy to slide from the groove if the semicircular groove at the free end of the u-shaped groove is not of a closed structure. The reason for the larger impact relationship is mainly that the separation resistance formed at the splicing connection of the sleeve and the rod body is larger, namely the splicing connection is too tight. Therefore, the half-round groove-shaped part at the free end of the u-shaped groove is of a closed structure, and the preservation survival rate of the cell sample can be ensured on a certain layer. In addition, if the movement is not well controlled when the sleeve is separated from the stem body, the sleeve and the stem body are suddenly separated, so that the cell slide is in contact with the inner wall of the sleeve, and the cell sample is damaged. Therefore, the connection structure of the two needs to be further optimized.

Embodiment as shown in fig. 1: the tightening structure is a conical ring 121 arranged on the outer wall of the conical rod 12, and the small diameter end of the conical ring 121 is close to the cell slide 13. Under this structure, the port inner wall of column chamber 21 forms line face cooperation relation with conical surface ring 121, can reduce the body of rod and the resistance when sleeve pipe breaks away from to a certain extent for the separation operation of the two is more steady, can avoid having big shake, has reduced the possibility of cell slide and the contact of cover inside pipe wall.

As shown in fig. 3 and 4, the tightening structure includes a collar 3, and a cylindrical rod section 122 formed at the root of the tapered rod 12 near the rod body 11, and the outer diameter of the cylindrical rod section 122 is smaller than the large outer diameter of the tapered rod 12. The collar 3 is fitted over the cylindrical rod section 122. The outer peripheral surface of the lantern ring 3, which is far away from one side of the rod body 11, is a conical surface a, the small-diameter end of the conical surface a faces outwards, and the other side of the conical surface a is provided with a radial flange. Both ends of the lantern ring 3 are limited by a shaft shoulder formed by the rod body 11 relative to the cylindrical rod section 122 and a shaft shoulder formed by the large outer diameter end of the conical rod 12 relative to the cylindrical rod section 122, and can only do limited reciprocating sliding motion relative to the cylindrical rod section 122 along the axial direction. The cylindrical cavity 21 of the sleeve 2 is provided with a section of conical cavity 22 at the end. The conical surface a can be inserted into the conical cavity 22, so that the outer conical surface of the conical surface a and the inner conical surface of the conical cavity 22 are pressed against each other to connect the sleeve 2 and the collar 3 together. As shown in fig. 4, the outer diameter of the radial flange portion of the collar 3 is larger than the outer diameter of the rod body 11. Thus, when the sleeve and the rod body are connected or separated, the sleeve ring can axially move relative to the sleeve or the cylindrical rod section by poking the radial flange towards different axial directions. The structure can further ensure the controllability and the stability of the separation operation of the sleeve and the rod body, and can not vibrate violently in the moment, thereby greatly reducing the contact probability of the cell slide and the inner wall of the sleeve and effectively reducing the possibility that the cell sample is damaged in the operation.

As shown in fig. 1 and 3, the other end of the rod body 11 is provided with a tab section 14, the tab section 14 has a rectangular cross-section, and an end block 141 is provided at a free end thereof. The joint between the two ends of the label section 14 and the end block 141 and the rod body 11 is formed with a step. When the label is used, the label is adhered and sleeved on the label section and cannot slide to the rod body or slide from one end of the end block when being separated from the label section due to the limitation of the step structures at the two ends.

The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. The present invention can be modified in many ways without departing from the spirit and scope of the present invention, and those skilled in the art can modify or change the embodiments described above without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which may be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims

1. The utility model provides a pole is carried in vitrification, includes the body of rod and sleeve pipe, and the body of rod is including setting up the cell slide glass in its pole body one end, be equipped with the toper pole between pole body and the cell slide glass, the one end that this toper pole and cell slide glass are connected is the path end, the sleeve pipe can overlap and put outside cell slide glass and the toper pole, its characterized in that: the cross section of the cell slide is in a rectangle-like shape; one side of the cell slide is provided with a u-shaped groove extending along the length direction of the cell slide, and the u-shaped groove is positioned in the middle of the cell slide in the width direction.

2. The vitrification freezing carrier bar of claim 1 wherein: the end of the u-shaped groove close to the free end of the cell slide is spaced from the end of the cell slide.

3. The vitrification freezing carrier bar of claim 2 wherein: the end part of the free end of the cell slide is provided with a black mark; one end of the u-shaped groove extends to the black mark part, and the end head of the groove at the end has a distance relative to the end part of the black mark part.

4. The vitrification freezing carrier bar of claim 2 or 3 wherein: the end of the groove body, extending to the position near the free end of the cell slide, of the u-shaped groove is in a semicircular groove shape, the corner between the bottom surface of the semicircular groove and the vertical surface is in a fillet shape, and the two ends of the fillet are tangent to the bottom surface and the vertical surface respectively.

5. The vitrification freezing carrier bar of any one of claims 1 to 3 wherein: the other end of the rod body is provided with a label section, the transverse section of the label section is of a rectangular structure, and the free end part of the label section is provided with an end block; steps are formed at the joint of the two ends of the label section, the end block and the rod body.

6. The vitrification freezing carrier bar of any one of claims 1 to 3 wherein: one end of the tapered rod leaning rod body is provided with a tightening structure, and the tightening structure can be matched with the inner wall of the end port of the cylindrical cavity of the sleeve, so that the rod body and the sleeve are tightly connected into a whole and can be separated from each other when axial force which is opposite to each other is applied.

7. The vitrification freezing carrier bar of claim 6 wherein: the tightening structure is a conical surface ring arranged on the outer wall of the conical rod, and the small-diameter end of the conical surface ring is close to the cell slide.

8. The vitrification freezing carrier bar of claim 6 wherein: the tightening structure comprises a sleeve ring and a cylindrical rod section formed at the root of the conical rod close to the rod body, and the outer diameter of the cylindrical rod section is smaller than the large outer diameter of the conical rod;

the lantern ring is sleeved on the cylindrical rod section; the outer peripheral surface of one side of the lantern ring, which is far away from the rod body, is a conical surface a, the small diameter end of the conical surface a faces outwards, and the other side of the lantern ring is provided with a radial flange;

the two ends of the lantern ring are limited by a shaft shoulder formed by the rod body relative to the cylindrical rod section and a shaft shoulder formed by the large outer diameter end of the conical rod relative to the cylindrical rod section, and can only do limited reciprocating sliding motion relative to the cylindrical rod section along the axial direction;

a section of conical surface cavity is formed at the end port of the cylindrical cavity of the sleeve; the conical surface a can be inserted into the conical surface cavity, so that the outer conical surface of the conical surface a and the inner conical surface of the conical surface cavity are mutually pressed to connect the sleeve and the sleeve ring together.

9. The vitrification freezing carrier bar of claim 8 wherein: the collar has a radial flange portion with an outer diameter greater than an outer diameter of the stem body.