CN210519936U

CN210519936U - Sealing sleeve

Info

Publication number: CN210519936U
Application number: CN201921241826.1U
Authority: CN
Inventors: 彭秋平; 薛松果; 王荣祥; 彭文林; 胥尧; 匡延平
Original assignee: Shanghai Lindian Industrial Co ltd
Current assignee: Jiangsu Ruifuda Medical Instrument Co ltd
Priority date: 2019-01-18
Filing date: 2019-08-02
Publication date: 2020-05-15
Anticipated expiration: 2029-08-02

Abstract

The utility model provides a sealing kit, which comprises an outer sleeve, a sealing sleeve and a sealing sleeve, wherein the outer sleeve is provided with an inner cavity, and a refrigerant is filled in the inner cavity; and the freezing carrier can carry a sample, is arranged in the inner cavity, and is sealed by the outer sleeve so as to seal the freezing carrier carrying the sample. Its advantage lies in, the outer sleeve pipe inner chamber is full of aseptic refrigerant earlier, and the freezing carrier that will load the sample again inserts the inner chamber, realizes that the vitrification freezes, and cooling rate is the same with open carrier, and the survival rate that unfreezes is the same with open carrier, the utility model discloses simple structure, embryologist can realize closed, aseptic, the high-efficient ultra-low temperature long-term storage of sample on the basis that does not basically change current open carrier operation custom.

Description

Sealing sleeve

Technical Field

The utility model relates to a medical treatment auxiliary instrument field especially relates to a sealed external member.

Background

Ultralow temperature freezing is one of the main methods for long-term preservation of biological materials such as embryos, gametes (including sperms and ova) and the like. Open rod carrier vitrification freezing methods (such as Cryotop, Cryoleaf, Strawtop, etc.) are widely used because of their high temperature reduction rate (above 20,000 ℃/min) and high thawing survival rate, but because embryos directly contact liquid nitrogen, they have potential contamination and cross-infection risks, and are always troubling practitioners of assisted reproduction. Closed vitrification freezing methods such as closed drawn straws (CPS) and Rapid-i have also been reported for freezing embryos clinically in order to avoid contamination, but the survival rate is poor and the methods are not widely used. The known closed vitrification freezing methods of CPS, Rapid-i and the like are that freezing carriers loaded with embryos are inserted into an outer sleeve to be sealed, then liquid nitrogen is put into the outer sleeve, the freezing speed of the embryos is slow (only about 2,000 ℃/min), and the thawing survival rate is low.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the technical problem that a sealed external member is provided, its sample that can avoid loading on the freezing carrier is polluted, and can realize the high survival rate the same with open carrier.

In order to solve the above problem, the utility model provides a sealing kit, it includes: an outer sleeve having an opening and an inner cavity, the inner cavity being adapted to be filled with a refrigerant, and the opening being sealable; a freezing carrier capable of carrying a sample, wherein the freezing carrier can be arranged in the inner cavity of the outer sleeve.

In one embodiment, the end of the outer sleeve has an opening through which the frozen carrier can be stored in the inner cavity, and the opening is locked to seal the outer sleeve when the frozen carrier with the sample is placed in the inner cavity.

In one embodiment, an inner thread is disposed on an inner side wall of the opening or an outer thread is disposed on an outer side wall of the opening, and a nut is engaged with the inner thread or the outer thread to seal the inner cavity.

In one embodiment, the side wall of the opening has at least one notch extending toward the center of the outer sleeve, and the side wall of the opening is attached with the notch as a boundary so that the opening is locked.

In one embodiment, the side wall of the opening is provided with two notches extending towards the center of the outer sleeve, the two notches are symmetrically arranged, and the side wall of the opening is attached by taking the notches as boundary lines so that the opening is locked.

In one embodiment, the sealing kit further comprises an indicator portion connected to an end of the outer sleeve having an opening, and the opening is exposed outside the indicator portion.

In one embodiment, a portion of the sidewall of the opening extends away from the outer sleeve to form the indicator.

In an embodiment, the marking portion includes a first section and a connecting section, two ends of the connecting section are respectively connected to the first section and the outer sleeve, and a width of the connecting section is smaller than a width of the first section so as to expose the opening of the outer sleeve.

In one embodiment, the end face of the first section is provided with a label for marking information of the sample carried by the frozen carrier.

In one embodiment, a filter element is disposed at the opening of the outer sleeve for filtering liquid or gas entering the inner chamber to remove microorganisms and impurities. The filter can be removed prior to placing the frozen carrier loaded with frozen sample in the overtube lumen.

The utility model has the advantages that:

(1) when the freezing carrier is used, the inner cavity of the outer sleeve is filled or prepared with the sterile refrigerant, and then the freezing carrier is inserted into the outer sleeve, so that the temperature reduction rate of a sample loaded on the freezing carrier is not obviously different from that of an open carrier (more than 20,000 ℃/min), and the thawing survival rate of the sample is not influenced.

(2) The refrigerant filled in the inner cavity of the outer sleeve can be obtained by filtering commercially available liquid nitrogen (unclean and bacteria) with a filter membrane, or can be derived from sterile liquid air prepared from sterile air treated by the filter membrane.

(3) After the freezing carrier is inserted into the inner cavity of the outer sleeve, the outer sleeve is sealed at the opening of the outer sleeve by ultrasonic sealing, heat sealing or spiral sealing, namely, the sample is stored in a sealed sterile ultralow temperature microenvironment, so that the pollution of commercial sterile liquid nitrogen or cross infection of other patient biological materials in the storage process can be thoroughly avoided.

(4) When unfreezing, expose the liquid nitrogen face with the opening of inner chamber, the scissors is directly cut open, takes out the freezing carrier and unfreezes, and the sample of loading on the freezing carrier can not contact there is the fungus liquid nitrogen, pollution-free risk.

Drawings

Fig. 1 is a schematic structural view of a first embodiment of the sealing sleeve of the present invention;

fig. 2 is a schematic structural view of a second embodiment of the sealing sleeve of the present invention;

fig. 3 is a schematic structural view of a third embodiment of the sealing sleeve of the present invention;

fig. 4 is a schematic structural view of a fourth embodiment of the sealing sleeve of the present invention.

Detailed Description

The following describes in detail a specific embodiment of the sealing kit provided by the present invention with reference to the accompanying drawings.

Fig. 1 is a schematic structural view of a first embodiment of the sealing sleeve of the present invention. Referring to fig. 1, the sealing kit of the present invention includes an outer sleeve 1 and a freezing medium 2.

The outer sleeve 1 has an inner cavity 10. The inner cavity 10 is filled with a refrigerant 11. Including but not limited to liquid nitrogen or liquid air. Preferably, the refrigerant is a sterile refrigerant.

The frozen carrier 2 is disposed in the inner cavity 10. Since the frozen carrier 2 is shielded by the outer sleeve 1, it is shown in fig. 1 by a dotted line. The freezing carrier 2 is a rod-shaped vitrified freezing carrier (such as Cryotop, Cryoleaf, etc.), which can be obtained from the prior art by those skilled in the art and will not be described in detail. The frozen carrier 2 is capable of carrying a sample. Such samples include, but are not limited to, human or animal embryos, zygotes, ova, sperm, tissues (e.g., ovarian tissue, testicular tissue, etc.), and the like, which require long-term cryopreservation of cells or tissues.

When the sample-bearing frozen carrier is placed in the inner cavity 10, the outer sleeve 1 is sealed to seal the sample-bearing frozen carrier 2. The refrigerant 11 is used to freeze the sample.

Specifically, in the present embodiment, the outer sleeve 1 is a tubular structure with one end sealed and the other end having an opening 12. That is, the opening 12 is provided at the end of the outer tube 1. The frozen carrier 2 can be stored in the inner cavity 10 through the opening 12, and when the frozen carrier 2 carrying the sample is placed in the inner cavity 10, the opening 12 is locked to make the outer sleeve 1 sealed.

There are many ways in which the opening 12 may be locked. For example, in the present embodiment, the opening 12 is ultrasonically locked. Specifically, an ultrasonic sealer is applied to the opening 12 to make the side walls of the opening 12 abut, and the opening 12 is locked. Wherein fig. 1 shows the structure of the opening 12 without locking. Further, in this embodiment, the side wall of the opening 12 has at least one notch 13 extending toward the center of the outer sleeve 1, for example, two symmetrically disposed notches 13, and the side wall of the opening 12 is attached with the notches 13 as boundary lines, so that the opening 12 is locked. Due to the existence of the cut-out 13, the side walls on both sides of the opening 12 are more easily kneaded, thereby facilitating the sealing. In other embodiments of the present invention, the notch 13 may not be provided, and the present invention is not limited to this. Wherein, the depth of the cut-out 13 can be set according to the actual requirement.

For example, in the second embodiment of the present invention, the opening 12 is locked by means of a screw seal. Fig. 2 is a schematic structural view of a second embodiment of the sealing sleeve of the present invention. Referring to fig. 2, the second embodiment of the sealing sleeve is different from the first embodiment of the sealing sleeve in that an external thread 121 is disposed on an outer side wall of the opening 12, a nut 122 is engaged with the external thread 121, and the opening 12 is locked to seal the inner cavity 10. In another embodiment of the present invention, an inner wall of the opening 12 may be provided with an inner thread, a nut is engaged with the inner thread, and the opening 12 is locked to seal the inner cavity 10.

In addition, in other embodiments of the present invention, the opening 12 may be locked by a method such as thermocompression, which is not described herein again. The material of the outer sleeve 1 may be transparent or opaque, and may be a plastic material or a metal material, which is not limited herein.

With continued reference to fig. 1, a filter element 14 is disposed in the inner cavity 10 of the outer sleeve 1 at the opening 12 to filter impurities entering the inner cavity 10. The filter element 14 may be a membrane filter or the like known in the art, which is capable of allowing gas to enter the interior 10, but does not allow impurities such as dust to enter the interior 10.

In this embodiment, a label may be attached to the outer sidewall of the outer sleeve 1 to record sample information, such as the name of the patient and the date of freezing. The label includes but is not limited to a conventional paper label or an RFID chip.

Further, the sealing kit further comprises a marking part 3, and a label can be attached to the marking part 3 to record sample information.

Fig. 3 is a schematic structural view of a third embodiment of the sealing sleeve of the present invention. Referring to fig. 3, in the present embodiment, the indicating portion 3 is connected to an end of the outer sleeve 1 having an opening 12, and the opening 12 is exposed outside the indicating portion 3. Specifically, a part of the side wall of the opening 12 extends in a direction away from the outer sleeve 1 to form the indicator 3 so that the indicator 3 does not block the opening 12. For example, a half side wall of the opening 12 extends away from the outer tube 1 to form the semi-circular arc-shaped index portion 3. Of course, in other embodiments of the present invention, a part of the sidewall of the opening 12 extends in a direction away from the outer sleeve 1, and the marking portion 3 with other radians may also be formed, which is not described herein again. A label is attached to the side wall of the label portion 3 to record sample information such as the name of the patient and the date of freezing. The label includes but is not limited to a conventional paper label or an RFID chip.

Fig. 4 is a schematic structural view of a fourth embodiment of the sealing sleeve of the present invention. Referring to fig. 4, in the present embodiment, the indicating portion 3 includes a first section 30 and a connecting section 31, and two ends of the connecting section 31 are respectively connected to the first section 30 and the outer sleeve 1. Wherein the width of the connecting section 31 is smaller than the width of the first section 30, so as to expose the opening 12 of the outer sleeve 1. Specifically, a part of the side wall of the opening 12 extends away from the outer sleeve 1 to form the connecting section 31 so that the marking portion 3 does not block the opening 12. For example, half of the side wall of the opening 12 extends away from the outer sleeve 1 to form a 240-degree arc-shaped connecting section 31. Of course, in other embodiments of the present invention, a portion of the sidewall of the opening 12 extends in a direction away from the outer sleeve 1, and the connecting section 31 with other radians may also be formed, which is not described herein again.

Wherein the end face of the first section 30 is provided with a label for marking information of the sample carried by the frozen carrier. In particular, the end face of the first section 30 facing away from the connecting section 31 is provided with a label. In order to facilitate the search for the sealing kit placed inside the liquid nitrogen tank. The length, shape, etc. of the first section 31 can be set according to actual requirements. Specifically, in the present embodiment, the first section 31 is a solid cylinder with a length of about 25 mm, and in other embodiments, the first section 31 may also be in the shape of a rectangular parallelepiped or a cone, or may also be a hollow cylinder with a length that can be adjusted according to the requirement of the outer sleeve 1.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and decorations can be made without departing from the principle of the present invention, and these improvements and decorations should also be regarded as the protection scope of the present invention.

Claims

1. A sealed kit for freezing and preserving a vitrified frozen sample, comprising:

an outer sleeve having an opening and an inner cavity, the inner cavity being adapted to be filled with a refrigerant, and the opening being sealable;

a freezing carrier capable of carrying a sample, wherein the freezing carrier can be arranged in the inner cavity of the outer sleeve.

2. The sealing kit of claim 1, wherein the end of the outer sleeve has an opening through which the frozen carrier can be placed in the inner cavity, and wherein when the frozen carrier with the sample is placed in the inner cavity, the opening is closed to seal the outer sleeve.

3. The seal kit of claim 2, wherein an internal thread is provided on an inner sidewall of the opening or an external thread is provided on an outer sidewall of the opening, and a nut is engaged with the internal thread or the external thread to seal the inner cavity.

4. The seal kit of claim 2, wherein the sidewall of the opening has at least one notch extending toward the center of the outer sleeve, the sidewall of the opening being attached about the notch to allow the opening to be locked.

5. The seal kit of claim 2, wherein the side wall of the opening has two notches extending toward the center of the outer sleeve, the two notches are symmetrically arranged, and the side wall of the opening is attached with the notches as boundary lines so that the opening is locked.

6. The sealing kit of claim 2, further comprising an indicator coupled to the end of the outer sleeve having the opening, wherein the opening is exposed beyond the indicator.

7. The sealing kit of claim 6, wherein a portion of the sidewall of the opening extends away from the outer sleeve to form the indicator.

8. The sealing kit as claimed in claim 6, wherein the indicator includes a first section and a connecting section, both ends of the connecting section are connected to the first section and the outer sleeve, respectively, and the width of the connecting section is smaller than that of the first section to expose the opening of the outer sleeve.

9. The sealing kit according to claim 8, characterized in that the end face of the head section is provided with a label for marking information of the sample carried by the frozen carrier.

10. The sealing kit of claim 1, wherein a filter is disposed at the opening of the outer sleeve for filtering the liquid or gas entering the inner cavity to remove microorganisms and contaminants.