CN210314271U

CN210314271U - Nucleic acid extraction equipment and detection system

Info

Publication number: CN210314271U
Application number: CN201921261840.8U
Authority: CN
Inventors: 王升启; 肖瑞; 荣振
Original assignee: Institute of Pharmacology and Toxicology of AMMS
Current assignee: Institute of Pharmacology and Toxicology of AMMS; Academy of Military Medical Sciences AMMS of PLA
Priority date: 2019-08-06
Filing date: 2019-08-06
Publication date: 2020-04-14
Anticipated expiration: 2029-08-06

Abstract

The utility model provides a nucleic acid extraction equipment and detecting system, relates to nucleic acid extraction technical field, nucleic acid extraction equipment includes the shell, is provided with nucleic acid in the shell and draws the cavity, and the shell is provided with the inlet port with nucleic acid extraction cavity intercommunication, installs the exhaust fan on the shell, and the exhaust fan is used for drawing the gaseous exhaust in the cavity with nucleic acid, and filter mechanism is installed to inlet port department. In carrying out the nucleic acid and extracting the operation in-process, the air supply fan discharges the gaseous emission in the nucleic acid extraction cavity to the shell outside, form the negative pressure in the nucleic acid extraction cavity, make gaseous via filter mechanism entering, thereby make gaseous and the shell outside in the nucleic acid extraction cavity gaseous can carry out gas exchange, with the material discharge such as the aerosol that produces in the nucleic acid extraction cavity, avoid aerosol to stay for a long time in the nucleic acid extraction cavity and disturb the extraction process, and the gaseous via filter mechanism filtration that gets into the nucleic acid extraction cavity, can avoid sending into the pollutant in the nucleic acid extraction cavity.

Description

Nucleic acid extraction equipment and detection system

Technical Field

The utility model relates to a nucleic acid draws the field, particularly, relates to a nucleic acid draws equipment and detecting system.

Background

Nucleic acid is the basic object of molecular biology research, and the nucleic acid extraction step is the basic link in molecular diagnosis. Therefore, the quality of nucleic acids extracted in the nucleic acid extraction process greatly affects the results of molecular diagnosis.

In the prior art, nucleic acid extraction is generally performed using a nucleic acid extractor. Nucleic acid extraction appearance includes the shell, and the shell inboard forms closed cavity, is provided with reagent in the closed cavity and holds mechanism and nucleic acid extraction mechanism, and reagent holds the mechanism and has a plurality of holding tanks, and nucleic acid extraction mechanism inserts a plurality of holding tanks of placing different reagents in proper order to carry out the nucleic acid extraction operation.

However, in the process of nucleic acid extraction, substances such as aerosol may be generated by reaction, and since the working chamber of the nucleic acid extraction instrument is a sealed environment, the substances such as aerosol may stay in the working chamber all the time after being generated, so that the contact time between the substances such as aerosol and the nucleic acid extraction mechanism is increased, the possibility of contamination of the nucleic acid extraction mechanism by aerosol is increased, and if the nucleic acid extraction mechanism is contaminated, the substances extracted by the nucleic acid extraction mechanism cannot obtain accurate detection results in the subsequent detection process, for example, viruses such as hepatitis a, hepatitis b, and the like cannot be detected.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a nucleic acid extraction equipment and detecting system, it can alleviate the pollution phenomenon of nucleic acid extraction in-process to a certain extent.

The embodiment of the utility model is realized like this:

the utility model provides a nucleic acid draws equipment, its includes the shell, be provided with the nucleic acid in the shell and draw the cavity, install air supply fan and exhaust fan on the shell, the air supply fan be used for to the nucleic acid draws the cavity air supply, the exhaust fan be used for with the interior gas outgoing of nucleic acid extraction cavity, air supply fan department installs filter mechanism.

In the preferred embodiment of the present invention, the filtering mechanism includes a mounting frame, at least two filtering nets are disposed on the mounting frame, and two adjacent filtering nets are filled with activated carbon particles.

In the preferred embodiment of the present invention, the filtering mechanism includes an installation frame, a sponge net is installed in the installation frame, the sponge net is embedded or coated with activated carbon, and at least one side of the sponge net is provided with a HEPA filter screen.

In the preferred embodiment of the present invention, the exhaust fan is connected to the housing through an exhaust pipe, and the diameter of the connection port between the exhaust pipe and the housing is smaller than the diameter of the connection port between the exhaust pipe and the fan.

In a preferred embodiment of the present invention, an extension pipe is connected to a side of the exhaust fan away from the housing.

In a preferred embodiment of the present invention, the air inlet and the exhaust fan are respectively installed on two opposite sides of the housing.

In the preferred embodiment of the present invention, the nucleic acid extracting chamber is provided with a sterilizing ultraviolet lamp inside.

In the preferred embodiment of the present invention, an air supply fan is disposed at the air inlet, the air supply fan is used for supplying air to the nucleic acid extracting chamber, and the filtering mechanism is disposed opposite to the air inlet or the air outlet of the air supply fan.

In the preferred embodiment of the present invention, the nucleic acid extraction chamber is provided with a reagent holding mechanism and a nucleic acid extraction mechanism, the reagent holding mechanism is installed in the sealing box, a plurality of isolation regions are provided in the sealing box, the sealing box separates the reagent holding mechanism into a plurality of regions, the sealing box is provided with a through hole, the nucleic acid extraction mechanism can stretch into the through hole, the through hole and there is a set distance between the openings of the reagent holding mechanism.

A detection system comprising the contamination-preventive nucleic acid extraction apparatus according to the above-mentioned technical aspect.

The embodiment of the utility model provides a beneficial effect is:

in carrying out the nucleic acid and extracting the operation in-process, the air supply fan is with shell external gas pump send to the nucleic acid extraction cavity, the air supply fan discharges the gas in the nucleic acid extraction cavity to the shell outside, thereby make the gas in the nucleic acid extraction cavity and shell outside gas can carry out gas exchange, discharge material such as the aerosol that produces in the nucleic acid extraction cavity, avoid aerosol to stay for a long time in the nucleic acid extraction cavity and disturb the extraction process, and the gas that gets into the nucleic acid extraction cavity via the air supply fan filters via filter mechanism, thereby can avoid sending the pollutant into in the nucleic acid extraction cavity.

In conclusion, the anti-pollution nucleic acid extraction equipment provided by the technical scheme can discharge pollutants in the nucleic acid extraction cavity and send the pollutants into cleaner gas in the working process, so that the condition of large-area infection caused by generation of substances such as aerosol in the nucleic acid extraction cavity can be reduced to a certain extent.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a schematic structural view (including an extension tube) of an anti-contamination nucleic acid extraction apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic structural view (excluding an extension tube) I of an anti-contamination nucleic acid extraction apparatus according to an embodiment of the present invention;

FIG. 3 is a perspective view showing the internal structure of a contamination-preventive nucleic acid extraction apparatus according to an embodiment of the present invention;

FIG. 4 is a schematic structural diagram (not including an extension tube) II of the anti-contamination nucleic acid extraction apparatus provided in the embodiment of the present invention;

FIG. 5 is a first schematic structural view of a seal box in the contamination-preventive nucleic acid extraction apparatus according to the embodiment of the present invention;

FIG. 6 is a second schematic structural view of a seal box in the contamination-preventive nucleic acid extraction apparatus according to the embodiment of the present invention.

In the figure:

10-a housing; 11-a door body; 12-a display screen; 13-a push-pull mechanism; 21-a blowing fan; 22-exhaust fan; 23-an exhaust pipe; 24-an extension tube; 30-sterilizing ultraviolet lamp; 40-sealing the box; 41-box body; 42-a cover body; 43-a through hole; 44-a separator; 45-fixture block; 50-a filter mechanism; 60-a reagent containment mechanism; 70-nucleic acid extraction mechanism.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of the embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate the position or positional relationship based on the position or positional relationship shown in the drawings, or the position or positional relationship that the products of the present invention are usually placed when used, and are only for convenience of description and simplification of the description, but do not indicate or imply that the nucleic acid extracting apparatus or element that is referred to must have a specific position, be constructed and operated in a specific position, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not imply that the components are required to be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Some embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The embodiments described below and the features of the embodiments can be combined with each other without conflict.

First embodiment

Referring to fig. 1-4, the present embodiment provides a nucleic acid extraction apparatus, which includes a housing 10, a nucleic acid extraction chamber is disposed in the housing 10, an air inlet and an exhaust fan 22 are installed on the housing 10, the air inlet is communicated with the nucleic acid extraction chamber, the exhaust fan 22 is used for exhausting air in the nucleic acid extraction chamber, and a filtering mechanism 50 is installed at the air inlet.

In a preferred embodiment, the exhaust fan 22 is 90mm in size, 24V in voltage, and 1.22A in current.

The filter mechanism 50 is used to filter the gas to improve the cleanliness of the gas entering the nucleic acid extraction chamber. The filter mechanism 50 may be used to filter dust, impurities, or PM2.5 particles in the air, or to filter toxic gases.

In one embodiment, the filter mechanism 50 may be configured as follows: the filtering mechanism 50 comprises an installation frame, at least two filter screens are arranged on the installation frame, activated carbon particles are filled between every two adjacent filter screens, the installation frame and the filter screens limit the activated carbon particles, and the activated carbon particles are used for filtering gas.

For example, be provided with two filter screens on the installing frame, two filter screens enclose with the installing frame and establish formation accommodation space, and this accommodation space fills has the active carbon granule. Further, can be provided with between two filter screens and separate the shelves spare, separate the shelves spare and divide into a plurality of regions with accommodation space, each region all fills has the active carbon granule, so sets up for the active carbon granule is more even in accommodation space's distribution.

Or, can also be provided with three filter screen on the installing frame, three filter screen can enclose with the installing frame and establish two accommodation space of formation, and two accommodation space all fill have the active carbon granule.

Further, the number of the filter screens can be more.

In another possible embodiment, the filtering mechanism 50 includes a mounting frame, a sponge net is mounted in the mounting frame, the sponge net is embedded with activated carbon particles or coated with an activated carbon coating, and furthermore, a HEPA filter net is disposed on at least one side of the sponge net. The edge and the installing frame of HEPA filter screen are connected, and the HEPA filter screen can only set up in one side of sponge net, also can all be provided with the HEPA filter screen in the both sides of sponge net. HEPA is called high efficiency particulate air Filter in English, Chinese meaning is high efficiency air Filter, reaches HEPA standard Filter screen, reaches 99.7% to 0.1 micron and 0.3 micron's effective rate, HEPA net's characteristics are that the air can pass through, but tiny particle can't pass through to can Filter at air intake department, do not block the air and get into through the air intake again. HEPA divides five materials of PP filter paper, glass fiber, compound PPPET filter paper, melt-blown polyester non-woven fabrics and melt-blown glass fiber, and its windage is little, and the dust holding capacity is big, and filter accuracy is high. In addition, both the sponge net and the activated carbon can filter the gas. The mounting frame is used to fix the sponge net to the casing 10 such that the sponge net is opposite to the air inlet or outlet of the blowing fan 21.

Further, the filtering mechanism 50 may further include a filtering net, and the filtering net may be disposed at one side of the sponge net, or at one side coated with activated carbon.

In an alternative embodiment, a blower fan 21 may be installed at the air inlet hole, the blower fan 21 is used for pumping the air outside the casing 10 into the nucleic acid extracting chamber, and the blower fan 21 and the exhaust fan 22 cooperate to accelerate the flow rate of the air in the nucleic acid extracting chamber.

The filter mechanism 50 may be installed on the air inlet side of the blower fan 21, or may be installed on the air outlet side of the blower fan 21.

When the filter mechanism 50 is attached to the air supply side of the air supply fan 21, as shown in fig. 3, the filter mechanism 50 is attached to the outside of the casing 10, the air supply fan 21 is attached to the inside of the casing 10, and the filter mechanism 50 faces the air inlet of the air supply fan 21.

When the filter mechanism 50 is mounted on the air outlet side of the blower fan 21, the filter mechanism 50 is mounted on the inner side of the casing 10, the blower fan 21 may be mounted on the inner side of the casing 10, or may be mounted on the outer side of the casing 10, and the filter mechanism 50 faces the air outlet of the blower fan 21.

In fig. 3, since the filter structure is installed at the air inlet to completely shield the air inlet, the air inlet is not visible, and the position of the air inlet can be estimated from the position of the filter structure, and in a preferred embodiment, the air inlet and the exhaust fan 22 are respectively installed at two opposite sides of the housing 10. When the air inlet holes are formed in the first side plate of the casing 10, the exhaust fan 22 is disposed on the second side plate. So set up, when gaseous in the inlet vent to exhaust fan 22 department flow, the whole nucleic acid extraction cavity is run through in the gas flow process, is favorable to the gas exchange of nucleic acid extraction cavity department.

In particular, the air inlet and the exhaust fan 22 may be disposed on two opposite side plates, and may be disposed in a staggered manner. For example, the air inlet is disposed in the middle region of the first side plate of the housing 10, the exhaust fan 22 is disposed in the middle region of the second side plate of the housing 10, and the central axis of the air inlet and the central axis of the exhaust fan 22 are located on the same horizontal plane. Alternatively, the air inlet is disposed in a lower region or a middle region of the first side plate, and the exhaust fan 22 is disposed in an upper region of the second side plate. Alternatively, the exhaust fan 22 is disposed in the lower area or the middle area of the second side plate, and the air inlet is disposed in the upper area of the first side plate.

Preferably, as shown in FIG. 3, the air inlet hole is provided in the region of the casing 10 opposed to the lower region of the nucleic acid extraction chamber, and the exhaust fan 22 is provided in the region of the casing 10 opposed to the upper region of the nucleic acid extraction chamber, so that the air flow in the nucleic acid extraction chamber is in a state of flowing obliquely upward under the action of the air inlet hole and the exhaust fan 22, so that the impurities generated in the reagent holding mechanism 60 located in the lower region of the nucleic acid extraction chamber move obliquely upward directly with the air flow and are discharged out of the nucleic acid extraction chamber, to reduce the possibility of the impurities moving laterally to other reagents.

As shown in fig. 1-4, the exhaust fan 22 is communicated with the casing 10 through an exhaust duct 23, and the diameter of a communication port between the exhaust duct 23 and the casing 10 is smaller than that between the exhaust duct 23 and the fan. That is, the exhaust pipe 23 is a reducer pipe, and the pumping force can be improved by using the exhaust pipe 23.

In the preferred embodiment of the present invention, an extension pipe 24 is connected to a side of the exhaust fan 22 away from the housing 10. One end of the extension pipe 24 is connected to an exhaust outlet of the exhaust fan 22, and the other end extends to the outside. Specifically, the contamination-preventive nucleic acid extraction apparatus is usually placed indoors, and the exhaust gas from the exhaust fan 22 can be discharged outdoors using the extension pipe 24, so that the flow of substances such as aerosol generated during the extraction process into the room can be prevented, the indoor air quality can be improved, and the workload of the filter mechanism 50 can be reduced.

In the preferred embodiment of the present invention, the sterilizing UV lamp 30 is disposed inside the nucleic acid extracting chamber. The ultraviolet sterilizing lamp 30 is used for sterilizing the nucleic acid extraction chamber. Specifically, the germicidal ultraviolet lamp 30 emits short-wave ultraviolet rays to kill or inactivate bacteria, viruses and microorganisms, thereby performing a sterilizing function.

In the preferred embodiment of the present invention, the nucleic acid extracting chamber is provided with a reagent containing mechanism 60 and a nucleic acid extracting mechanism 70, and the irradiation range of the ultraviolet sterilizing lamp 30 includes the reagent containing mechanism 60 and the nucleic acid extracting mechanism 70 and the region therebetween.

Further, the number of the ultraviolet sterilizing lamps 30 is one or more, as shown in fig. 3, in one embodiment, a plurality of ultraviolet sterilizing lamps 30 are disposed in the nucleic acid extracting chamber, the ultraviolet sterilizing lamps 30 are divided into two groups, and the two groups of ultraviolet sterilizing lamps 30 are respectively mounted on two opposite inner walls of the nucleic acid extracting chamber. Each set of germicidal ultraviolet lamps 30 may include a plurality of germicidal ultraviolet lamps 30, two germicidal ultraviolet lamps 30 per set being illustrated in fig. 3 as an example.

Specifically, the germicidal ultraviolet lamp 30 may be a lamp tube capable of emitting ultraviolet light; or a lamp tube group consisting of a plurality of lamp tubes capable of emitting ultraviolet light; or, the LED lamp bead or the LED paster that can send ultraviolet light can also be constituteed by a plurality of.

As shown in FIGS. 3, 5 and 6, in order to avoid cross-contamination between different reagents in the actual containing mechanism, it is preferable that the reagent containing mechanism 60 is installed in a sealed box 40, a plurality of isolation regions are provided in the sealed box 40, the sealed box 40 divides the reagent containing mechanism 60 into a plurality of regions, the sealed box 40 is provided with a through hole 43, the nucleic acid extracting mechanism 70 can protrude into the through hole 43, and a set distance exists between the through hole 43 and the opening of the reagent containing mechanism 60. With this arrangement, even if the reagent in one isolation region generates aerosol and other substances, the substances will move along the isolation region to the through hole 43 instead of directly scattering to the reagent beside as in the prior art, so that the reagent in other isolation regions can be prevented from cross-infection. The aerosol or the like discharged from the through hole 43 flows upward obliquely with the air flow in the nucleic acid isolation mechanism 70, and is discharged from the exhaust fan.

Specifically, the sealing case 40 may have the following structure: the sealing box 40 includes a box body 41 and a cover body 42, a partition 44 is installed on the box body 41, a through hole 43 is installed on the cover body 42, a latch 45 is disposed on the outer side of the box body 41, a slot is disposed on the cover body 42, and the box body 41 and the cover body 42 are engaged with each other through the engagement of the latch 45 and the slot.

The box body 41 may be provided with a fixing frame for fixing the reagent holding mechanism 60, or the box body 41 may be provided with a socket for extending the bottom region of the reagent holding mechanism 60, and the opening of the reagent holding mechanism 60 is located in the isolation region of the box body 41.

Further, the bottom of the reagent holding mechanism 60 is fixed to the push-pull mechanism 13, and the push-pull mechanism 13 is slidably fitted to the housing and can be pulled out of the nucleic acid extraction chamber.

In addition, a door 11 is arranged on the shell 10 in a region opposite to the nucleic acid extraction chamber, and the door 11 is made of transparent or semitransparent materials.

The housing 10 may further be provided with a display 12, and the display 12 may be used to display information such as the nucleic acid extraction process, relevant parameters (e.g., temperature, humidity, air quality, etc.) in the nucleic acid extraction chamber, etc. The display 12 may be a touch screen through which control information may be input.

Second embodiment

This embodiment provides a detection system including the nucleic acid extraction apparatus provided in the first embodiment described above.

Further, the detection system may further include a detection device for detecting the substance extracted by the nucleic acid extraction device.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides a nucleic acid extraction equipment, its characterized in that, which comprises an outer shell, be provided with the nucleic acid extraction cavity in the shell, the shell be provided with the inlet port of nucleic acid extraction cavity intercommunication, install the exhaust fan on the shell, the exhaust fan be used for with the gaseous emission in the nucleic acid extraction cavity, filter mechanism is installed to inlet port department.

2. The nucleic acid extraction apparatus according to claim 1, wherein the filter mechanism includes a mounting frame, at least two filter screens are disposed on the mounting frame, and activated carbon particles are filled between two adjacent filter screens.

3. The nucleic acid extraction apparatus according to claim 1, wherein the filtering mechanism includes a mounting frame, a sponge net is mounted in the mounting frame, the sponge net is embedded or coated with activated carbon, and at least one side of the sponge net is provided with a HEPA filter screen.

4. The nucleic acid extraction apparatus according to claim 1, wherein the exhaust fan communicates with the casing via an exhaust duct, and a communication opening between the exhaust duct and the casing has a diameter smaller than a diameter of a communication opening between the exhaust duct and the fan.

5. The nucleic acid extraction apparatus according to claim 1, wherein an extension tube is connected to a side of the exhaust fan facing away from the housing.

6. The nucleic acid extraction apparatus according to claim 1, wherein the air intake hole and the exhaust fan are respectively installed at opposite sides of the housing.

7. The nucleic acid extraction apparatus of claim 1, wherein the nucleic acid extraction chamber is provided with a germicidal ultraviolet lamp.

8. The nucleic acid isolation apparatus according to claim 1, wherein an air supply fan is provided at the air inlet, the air supply fan supplying air to the nucleic acid isolation chamber, and the filter mechanism is provided opposite to an air inlet or an air outlet of the air supply fan.

9. The nucleic acid extraction apparatus according to claim 1, wherein the nucleic acid extraction chamber is provided with a reagent holding mechanism and a nucleic acid extraction mechanism, the reagent holding mechanism is installed in a sealed box, a plurality of isolation regions are provided in the sealed box, the sealed box divides the reagent holding mechanism into a plurality of regions, the sealed box is provided with a through hole into which the nucleic acid extraction mechanism can extend, and a set distance exists between the through hole and the opening of the reagent holding mechanism.

10. A detection system comprising the nucleic acid extraction apparatus of any one of claims 1 to 9.