CN210269860U - Novel detection card - Google Patents
Novel detection card Download PDFInfo
- Publication number
- CN210269860U CN210269860U CN201921279393.9U CN201921279393U CN210269860U CN 210269860 U CN210269860 U CN 210269860U CN 201921279393 U CN201921279393 U CN 201921279393U CN 210269860 U CN210269860 U CN 210269860U
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- Prior art keywords
- sample
- liquid feeding
- feeding hole
- hole
- aperture
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- 238000001514 detection method Methods 0.000 title claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 63
- 238000012360 testing method Methods 0.000 claims abstract description 32
- 241000463219 Epitheca Species 0.000 claims abstract description 12
- 239000012898 sample dilution Substances 0.000 claims abstract description 11
- 238000003491 array Methods 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 239000003085 diluting agent Substances 0.000 abstract description 10
- 230000035945 sensitivity Effects 0.000 abstract description 8
- 238000013461 design Methods 0.000 abstract description 6
- 238000006243 chemical reaction Methods 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 3
- 238000007789 sealing Methods 0.000 abstract description 3
- 230000008595 infiltration Effects 0.000 abstract description 2
- 238000001764 infiltration Methods 0.000 abstract description 2
- 241000220317 Rosa Species 0.000 abstract 1
- 238000007865 diluting Methods 0.000 description 5
- 238000012123 point-of-care testing Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000000853 adhesive Substances 0.000 description 3
- 230000001070 adhesive effect Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Images
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a novel detection card wholly is the bar form, including epitheca, inferior valve and the test paper strip of setting between epitheca and inferior valve, the epitheca includes sample dilution liquid feeding hole, sample liquid feeding hole and observation window, sample liquid feeding hole forms and dilutes between liquid feeding hole and observation window at the sample, the inside wall that the liquid feeding hole was diluted to the sample is square hourglass hopper-shaped, and its bottom towards the test paper strip is for sealing the form and having seted up a plurality of aperture. Implement the utility model discloses, through the bottom with sample dilution liquid feeding hole for sealing the form and having seted up a plurality of aperture, the form of seting up the aperture is designed into to the bottom and all compares for the form of through-hole with the bottom among the prior art, can the infiltration liquid speed of effective control diluent, because if the diluent application of sample volume is bigger, very easy washover, the time of reaction is short, the color development can receive the influence, through the design of aperture, slowly control the velocity of flow of liquid, reach the mesh that the sensitivity rose at last, improve the back than improve a high colourity of prerequisite.
Description
Technical Field
The utility model relates to a biological detection technical field, more specifically relate to a novel detect card.
Background
In vitro diagnostic reagents, particularly items detected by using a detection card, when a trace sample (such as blood, urine and the like) is detected, the sample needs to be diluted and mixed uniformly and then detected, so that the detection accuracy and precision can be improved.
SUMMERY OF THE UTILITY MODEL
In order to solve the not enough of prior art, the utility model provides a novel detection card through the poroid mode with the sample dilution liquid feeding hole design into particular structure, can the velocity of flow of effective control liquid, finally reaches the mesh that the sensitivity that detects rises, can improve a colourity.
The utility model discloses the technological effect that will reach is realized through following scheme: the utility model provides a novel detection card, wholly is the bar form, includes epitheca, inferior valve and sets up the test paper strip between epitheca and inferior valve, the epitheca includes sample dilution liquid feeding hole, sample liquid feeding hole and observation window, the sample liquid feeding hole forms between sample dilution liquid feeding hole and observation window, the inside wall that the sample diluted the liquid feeding hole is square hourglass hopper-shaped, and its bottom towards the test paper strip is for the closed form and seted up a plurality of aperture.
Preferably, the number of the small holes is three, and the small holes are arranged in a linear array in the length direction of the upper shell.
Preferably, the pore size of each of said pores is the same.
Preferably, the aperture of each said aperture is in the range 1mm to 4 mm.
Preferably, the bottom end of the sample dilution liquid adding hole is 0.1 mm-1 mm away from the gap between the test strips.
Preferably, the upper case and the lower case are fixed by snap-fitting.
Preferably, the novel detection card further comprises a plurality of air holes.
Preferably, the number of the air holes is 6, two rows are formed in the width direction of the upper shell, and each row is provided with 3 arrays.
Preferably, the sample diluting liquid adding hole, the sample liquid adding hole, the observation window and the air vent are sequentially arranged in a straight line shape on the upper shell in the length direction of the detection card.
The utility model has the advantages of it is following:
1. compared with the mode that the bottom ends of the sample diluting and liquid adding holes are all through holes in the prior art, the mode that the bottom ends of the sample diluting and liquid adding holes are closed and provided with the small holes can effectively control the seepage speed of the diluent, because the diluent is easy to wash away if the sample adding amount of the diluent is large, the reaction time is short, the color development can be influenced, the flow speed of the liquid is slowly controlled through the design of the small holes, the purpose of sensitivity increase is finally achieved, and the improved color is one color higher than that of the improved condition;
2. the double-hole arrangement of the sample dilution liquid adding hole and the sample liquid adding hole enables the higher detection sensitivity to be achieved by using less sample amount, the interference of other substances in the sample is reduced due to the reduction of the sample amount, the detection precision and accuracy are improved, the quality standard of quantitative diagnosis is suitable, and an achievable basis is provided for point-of-care testing (POCT);
3. the surface of the inner side wall of the sample liquid adding hole and the horizontal surface of the upper shell form an included angle of 80-90 degrees, so that when a sample is dripped, the sample is smoothly injected onto the test strip by virtue of gravity and surface adhesive force, the liquid residue on the hole wall is reduced, and the detection accuracy and tightness are improved.
Drawings
Fig. 1 is a schematic plan view of a novel detection card of the present invention;
fig. 2 is a schematic side view of a novel detection card of the present invention showing an internal structure in the longitudinal direction;
fig. 3 is the utility model discloses in a novel show sample liquid feeding hole and test paper strip structure on the detection card width direction look sideways at the schematic diagram.
Detailed Description
The invention is described in detail below with reference to the drawings, wherein examples of the embodiments are shown in the drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.
In the description of the present invention, it is to be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings for convenience in describing the present invention and for simplicity in description, and are not intended to indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.
Furthermore, the terms "first", "second", "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," or "third" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and "disposed" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral part; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or may be connected through the interconnection of two elements or through the interaction of two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
As shown in fig. 1-2, the embodiment of the utility model provides a novel detection card wholly is the strip form, novel detection card includes epitheca 10, inferior valve 20 and sets up the test paper strip 30 between epitheca 10 and inferior valve 20, epitheca 10 includes that the sample dilutes liquid feeding hole 11, sample liquid feeding hole 12 and observation window 13, sample liquid feeding hole 12 forms between sample dilutes liquid feeding hole 11 and observation window 13. The double-hole arrangement of the sample dilution liquid adding hole 11 and the sample liquid adding hole 12 enables higher detection sensitivity to be achieved with fewer sample volumes, interference of other substances in a sample is reduced due to the reduction of the sample volumes, detection precision and accuracy are improved, the method is suitable for quality standards of quantitative diagnosis, and a basis for realizing point-of-care testing (POCT) is provided.
The embodiment of the utility model provides an in, the inside wall that the liquid feeding hole 11 was diluted to the sample is square hourglass hopper-shaped, its bottom towards the test paper strip is for sealing the form and having seted up a plurality of aperture 111, the form that sets up aperture 111 is designed to the bottom and all compares for the form of through-hole in the prior art, can the infiltration liquid speed of effective control diluent, because if the diluent application of sample volume is bigger, it is very easy to wash over, the time of reaction is short, the color development can receive the influence, design through aperture 111, the velocity of flow of slowly controlling liquid, reach the mesh that the sensitivity rises at last, improve the back than improving a higher colourity of prerequisite. More preferably, the number of the small holes 111 is three, and the small holes are linearly arranged in the longitudinal direction of the upper casing 10, which provides the best effect of controlling the liquid permeation speed.
As a further improvement of the embodiment of the present invention, the aperture of each of the small holes 111 is preferably the same, and the aperture range of each of the small holes 111 is 1mm to 4 mm.
The distance between the bottom end of the sample dilution liquid adding hole 11 and the test strip is 0.1 mm-1 mm.
The upper shell 10 and the lower shell 20 are preferably assembled and fixed through a buckle, the upper shell 10 and the lower shell 20 are used for placing and fixing the test strip 30, and the assembly is rapid and convenient.
The embodiment of the present invention provides a detection reaction device for detecting corresponding target substance in vitro, which is prepared by the test strip 30 using fluorescence immunochromatography. After the sample and the sample diluent are respectively added, part of the sample starts immune combination reaction with the fluorescent conjugate under the condition of not being diluted, the sample diluent can elute the sample remained in the sample liquid adding hole 12 by means of siphon action and can assist the sample and the immune complex to move forward, so that the sample can fully participate in detection reaction, and a fluorescent signal line is formed at a detection line or a quality control line.
As shown in fig. 3, in the embodiment of the present invention, the inner sidewall surface of the sample liquid adding hole 12 is smooth, preferably, the inner sidewall surface is a flat plane, and the inner sidewall surface and the horizontal surface of the upper shell 10 form an included angle d of 80-90 degrees, so that when the sample is added dropwise, the sample is smoothly injected onto the test strip 30 by gravity and surface adhesion, thereby reducing the liquid residue on the hole wall and improving the accuracy and tightness of the detection; the clearance D between the lower edge of the inner side wall of the sample liquid adding hole 12 and the test strip 30 is 0-1mm, the clearance D can reduce the spreading resistance of the sample liquid, and meanwhile, the residue on the wall of the sample liquid adding hole 12 can be reduced by utilizing the surface adhesive force of the sample, so that the detection accuracy and tightness are improved; the width W of the sample liquid adding hole 12 is 2.5mm-4.0mm, the width W is +/-1 mm of the width of the test strip 30, the sample can be fully spread on the test strip 30 by utilizing the surface adhesive force of the sample liquid and cannot overflow out of the test strip 30, the liquid adding amount of the sample is ensured to be constant, and the accuracy and precision of detection are also improved. In addition, the gap D between the lower edge of the inner side wall of the sample liquid adding hole 12 and the test strip 30 is 0-1mm, and the width W of the sample liquid adding hole 12 is +/-1 mm of the width of the test strip 30, so that liquid surface viscous force and three-dimensional space capillary action can be utilized, an upwelling phenomenon occurs when sample diluent in the sample diluting liquid adding hole 11 flows through the sample liquid adding hole 12, residual samples on the inner side wall of the sample liquid adding hole 12 are automatically eluted in a moving mode, and the accuracy and precision of detection are improved.
The embodiment of the utility model provides a through adopting diplopore setting and sample liquid feeding hole 12 shape and size design for fluorescence quantitative determination sensitivity can reach the detection level of 10pg/ml level, detects precision (CV) simultaneously and is less than 15%, and the sample demand is few, effectively improves sensitivity and precision.
As a further improvement of the embodiment of the utility model, the novel detection card still includes a plurality of bleeder vent 14 for exhaust when the paper end liquid that absorbs water of test paper strip 30 evaporates, reduce the paper end liquid refluence phenomenon that absorbs water because of the evaporation of membrane face liquid. Preferably, the number of the ventilation holes 14 is 6, and the ventilation holes are arranged in two rows of 3 arrays in the width direction of the upper case 10, but not limited thereto.
As shown in figure 1, the sample diluting liquid adding hole 11, the sample liquid adding hole 12, the observation window 13 and the air vent 14 are sequentially arranged in a straight line on the upper shell 10 in the length direction of the detection card, and the structural design is simple and reasonable.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the embodiments of the present invention and not for limiting the same, and although the embodiments of the present invention are described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the embodiments of the present invention, and these modifications or equivalent substitutions cannot make the modified technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (9)
1. The utility model provides a novel detection card wholly is the bar form, includes epitheca, inferior valve and sets up the test paper strip between epitheca and inferior valve, its characterized in that, the epitheca dilutes liquid feeding hole, sample liquid feeding hole and observation window including the sample, the sample liquid feeding hole forms and dilutes between liquid feeding hole and observation window at the sample, the inside wall that the liquid feeding hole was diluted to the sample is square hourglass hopper-shaped, and its bottom towards the test paper strip is for the closed form and has seted up a plurality of aperture.
2. The novel test card of claim 1, wherein the number of said apertures is three, and said apertures are arranged in a linear array along the length of the upper housing.
3. A novel test card as claimed in claim 1 or 2 wherein the apertures of each said aperture are the same.
4. A novel test card as claimed in claim 1 or 2 wherein each of said wells has a pore size in the range of 1mm to 4 mm.
5. The novel test card of claim 1 or 2, wherein the bottom end of the sample dilution well is spaced from the test strip by a distance of 0.1 mm to 1 mm.
6. The novel test card of claim 1, wherein the upper and lower shells are secured by snap-fit.
7. The novel test card of claim 1, further comprising a plurality of vents.
8. The novel test card of claim 7, wherein the number of the vent holes is 6, and the vent holes are arranged in two rows of 3 arrays in the width direction of the upper case.
9. The novel test card of claim 7, wherein the sample dilution hole, the sample addition hole, the observation window and the air hole are arranged in a straight line on the upper shell of the test card in the longitudinal direction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921279393.9U CN210269860U (en) | 2019-08-08 | 2019-08-08 | Novel detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921279393.9U CN210269860U (en) | 2019-08-08 | 2019-08-08 | Novel detection card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210269860U true CN210269860U (en) | 2020-04-07 |
Family
ID=70016513
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921279393.9U Active CN210269860U (en) | 2019-08-08 | 2019-08-08 | Novel detection card |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210269860U (en) |
-
2019
- 2019-08-08 CN CN201921279393.9U patent/CN210269860U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210709 Address after: 518102 6th floor, building B, Xinzheng factory, area 71, Xingdong community, Xin'an street, Bao'an District, Shenzhen City, Guangdong Province Patentee after: Shenzhen xiupu Biotechnology Co.,Ltd. Address before: 518102 Yirui Biological Park, 2-1 Liuxian 1st Road, Xin'an street, Bao'an District, Shenzhen City, Guangdong Province Patentee before: SHENZHEN BIOEASY BIOTECHNOLOGY Co.,Ltd. |
|
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Zhuhai Ete Biotechnology Co.,Ltd. Assignor: Shenzhen xiupu Biotechnology Co.,Ltd. Contract record no.: X2024980009082 Denomination of utility model: A new type of detection card Granted publication date: 20200407 License type: Common License Record date: 20240708 |