CN210136174U

CN210136174U - Inverted small confocal micro-Raman system based on ship foundation

Info

Publication number: CN210136174U
Application number: CN201920413121.7U
Authority: CN
Inventors: 郭飞; 卢渊
Original assignee: Ocean University of China
Current assignee: Ocean University of China
Priority date: 2019-03-29
Filing date: 2019-03-29
Publication date: 2020-03-10
Anticipated expiration: 2029-03-29

Abstract

The utility model discloses a small-size confocal micro-raman system of inversion based on ship base belongs to optical instrument technical field. The utility model discloses based on the ship base design, facing to the demand of marine microorganism normal position real-time detection, do not adopt the light path design from top to bottom of the probing light of traditional confocal micro-raman instrument, but adopt the light path design of the inversion of optimization, its objective table is located the exciting light, make system main part and experiment platform can extremely short branch meet, greatly improved system stability under the prerequisite of guaranteeing signal intensity; and the compact light path design greatly reduces the volume and the weight of the system and improves the portability of the system. The utility model discloses low in cost, make things convenient for the scientific research personnel to carry, can carry out normal position real-time ground detection to the marine microorganism of on-the-spot collection on the scientific research ship.

Description

Inverted small confocal micro-Raman system based on ship foundation

Technical Field

The utility model relates to an optical instrument technical field, in particular to confocal micro-Raman system, this system can carry out normal position to marine microorganism and observe and detect in real time based on the ship base.

Background

Raman spectroscopy is a scattering spectrum that was discovered by Raman, a physicist of india in 1928. Raman spectroscopy is widely used as an analytical test means for molecular structures of substances, and is widely used in the fields of astronomy, food, macromolecules, pharmacy, biology, medicine and the like. The Raman spectrum is a nondestructive analysis technology, can carry out real-time detection, can effectively avoid the complex sample pretreatment process in the traditional cell biology method, and can also obtain the specific structure information of the biological components in the cells from the molecular level, so that the Raman spectrum is very suitable for the analysis of the microorganism samples.

Confocal microscopy Raman spectroscopy is developed on the basis of Raman spectroscopy technology, has the advantages of inhibiting fluorescence and improving detection resolution besides the advantages of Raman spectroscopy, is applied to single cell research as early as 1990 by G.J. Puppels et al, is regarded as one of ideal means for microbial analysis at the present stage and is in continuous development progress.

The sea is the source of life and is also a natural treasure house of human material resources. While the marine microorganisms including bacteria, actinomycetes, fungi and viruses provide half of the primary productivity on earth, affect the climate change, participate in the global material circulation and energy flow, and the research on marine microorganisms is a necessary development trend in the 21 st century. Due to the specificity of marine environment, a laboratory lacks a proper culture and analysis method, the research progress on marine microorganisms is slow, and the best stage of the research on the marine microorganisms at present is to collect in-situ real-time analysis on a research ship.

Confocal micro-Raman instruments produced commercially at the present stage are large in size and inconvenient for scientific researchers to carry and transport between boarding and disembarking; the maneuverability and the stability are poor, and stable detection is difficult to carry out on a scientific research ship with a bumpy position above the ocean. Therefore, it is highly desirable to design and develop a compact confocal micro-raman system with high stability, portability and mobility.

Disclosure of Invention

To the problem that above-mentioned prior art exists, the utility model discloses aim at designing one kind and use towards the ship base, the high small-size confocal micro-raman system of portability, stability can carry out normal position analysis in real time to the marine microorganism of on-the-spot taking on the scientific research ship.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

an inverted small confocal micro-Raman system based on a ship base comprises the following structures which are sequentially arranged on an optical path: a laser and a filter plate group; the focusing module comprises a microscope objective and an objective table; the confocal module comprises a convex lens A, a confocal pinhole and a convex lens B; the signal collection module comprises a convex lens C, a light coupler, an optical fiber and a spectrometer; the lighting module comprises an LED, a convex lens D and a ground glass sheet; and the imaging module comprises a convex lens E and a camera. The laser and the filter plate group are connected with the reflector B in a light path mode through the reflector A; the filter plate group is connected with the focusing module in a light path mode through a reflector C; the illumination module and the imaging module are optically connected by a beam splitter.

The laser is a small 532nm solid continuous laser with a maximum power of 200 mw.

The filter plate group comprises two identical long-pass filter plates, the transmission wavelength of the long-pass filter plates is greater than 532nm, and the reflection wavelength of the long-pass filter plates is less than or equal to 532 nm.

The microscope objective has a magnification of 50 times and a numerical aperture NA =0.55, and is located below the stage.

The stage is vertically movable and is positioned above the microscope objective.

The microscope objective is a 60-time water immersion microscope objective, and the numerical aperture NA = 1.

The confocal pinhole size is 400 microns and is located at the common focus of the front and rear convex lenses.

The LED is a blue light source.

The ratio of reflection to transmission of light by the beam splitter is 8: 92.

the utility model has the advantages of it is following:

1. the utility model discloses the light path design adopts inversion structure, and the objective table is located the top of light path, and the exciting light up shines on the object that awaits measuring from down for system's main part can meet with extremely short supporting rod with the experiment platform, has greatly improved the stability of system.

2. The utility model discloses the light path design is compact for the whole volume of system reduces, has improved its portability.

3. The utility model discloses use blue LED as light source, its formation of image light can be complete through the filter for the microorganism composition is surveyed and is gone on simultaneously with the microorganism formation of image.

4. The utility model discloses adopt small-size device in the device selection, reduced system's volume and system's quality, convenient transportation is carried.

5. The utility model discloses the light path design is nimble, and mobility is high, can improve at any time according to the scientific research personnel demand.

6. The utility model discloses whole cost is low, compares the commercialization instrument and has greatly reduced the cost.

7. The utility model discloses simple to use can supply zero basis personnel to use.

8. The utility model discloses can conveniently carry out normal position at the ship base and carry out the analysis in real time to the marine microorganism of on-the-spot collection.

Description of the drawings:

fig. 1 is a schematic view of the light path of the present invention.

In the figure: 1 is a laser; 2 is mirror A; 3, a reflector B; 4 is filter A; 5 is a mirror C; 6 is a beam splitter; 7 is a microscope objective; 8 is an object stage; 9 is a filter segment B; 10 is a convex lens A; 11 is a pinhole; 12 is a convex lens B; 13 is a convex lens C; 14 is a fiber coupler; 15 is a spectrometer; 16 is an LED lamp; 17 is a convex lens D; 18 is a ground glass sheet; 19 is a convex lens E; and 20 is a camera.

Fig. 2 is a schematic diagram of a raman spectrum analysis result output according to an embodiment of the present invention.

The specific implementation mode is as follows:

in order to make the objects, technical solutions and advantages of the present invention more clear, the present invention is further described in detail below with reference to the accompanying drawings. As shown in fig. 1, a ship-based inverted compact confocal micro-raman system includes a laser; a filter plate group; a focusing module: comprises a microscope objective and an objective table; a confocal module: the confocal pinhole lens comprises a convex lens A, a confocal pinhole and a convex lens B; a signal collection module: the device comprises a convex lens C, a light coupler, an optical fiber and a spectrometer; an illumination module: comprises an LED, a convex lens D and a ground glass sheet; an imaging module: comprises a convex lens E and a camera. The laser and the filter plate group are connected with the reflector B in a light path mode through the reflector A; the filter plate group is connected with the focusing module in a light path mode through a reflector C; the illumination module and the imaging module are optically connected by a beam splitter. Laser emitted from the laser 1 is reflected by a reflector A2, a reflector B3, a filter 4 and a reflector C4, passes through a beam splitter 6, reaches a microscope objective 7, is focused on a sample to be detected on an objective table 7, and generates Raman scattering light. The Raman scattered light is collimated by a microscope objective 7, passes through a beam splitter 6, is reflected by a reflector C5, is filtered by a filter A4 and a filter B5 to remove light except signal light, is focused to a pinhole 11 by a convex lens A10 to remove interference light except a detection point, is collimated by a convex lens B12, is focused to an optical fiber coupler 14 by a convex lens C13 and is transmitted to a spectrometer 15 by an optical fiber to finish the detection of the signal light. The LED light 16 emits illumination light, the illumination light is collimated by the convex lens D17, the ground glass sheet 18 illuminates an object to be detected on the objective table 7 after the object to be detected is homogenized, the image light is collimated by the microscope objective 7, reflected by the beam splitter 6 to reach the convex lens E19 for focusing, and enters the camera 20 to image the object to be detected.

As shown in fig. 2, select for use marine vibrio to be the analysis object, external spectrum appearance is ANDOR Kymera 328i spectrum appearance and Newton EMCCD, and is right the utility model discloses a detection analysis ability tests, presents the signal that the spectrum appearance was collected on the computer, removes baseline and analysis with the spectrogram, has found most raman peak position that the cell exists, proves the utility model discloses it is good to marine microorganism composition detection ability.

Claims

1. The inverted small confocal micro-Raman system based on the ship base is characterized by comprising the following structures which are sequentially arranged on an optical path: a laser and a filter plate group; the focusing module comprises a microscope objective and an objective table; the confocal module comprises a convex lens A, a confocal pinhole and a convex lens B; the signal collection module comprises a convex lens C, a light coupler, an optical fiber and a spectrometer; the lighting module comprises an LED, a convex lens D and a ground glass sheet; and the imaging module comprises a convex lens E and a camera.

2. The ship-based inverted compact confocal micro-raman system according to claim 1, wherein the laser and the filter set are optically connected with a reflecting mirror a and a reflecting mirror B; the filter plate group is connected with the focusing module in a light path mode through a reflector C; the illumination module and the imaging module are optically connected by a beam splitter.

3. The ship-based inverted compact confocal micro-raman system according to claim 1, wherein said laser is a small 532nm solid continuous laser with a maximum power of 200 mw.

4. The ship-based inverted compact confocal micro-raman system according to claim 1, wherein said filter set comprises two identical long pass filters that transmit light with a wavelength greater than 532nm and reflect light with a wavelength less than or equal to 532 nm.

5. The boat-based inverted compact confocal micro-raman system according to claim 1, wherein in the focusing module, the microscope objective has a magnification of 50 times and a numerical aperture NA =0.55, and is located below the stage.

6. The ship-based inverted compact confocal micro-raman system according to claim 1, wherein the stage is vertically movable in the focusing module above the microscope objective.

7. The ship-based inverted compact confocal micro-raman system according to claim 1, wherein in the confocal module, the confocal pinhole size is 400 μm, and is located at the common focus point of the front and rear convex lenses.

8. The ship-based inverted compact confocal micro-raman system according to claim 1, wherein in the illumination module, the LED is a blue light source.

9. The ship-based inverted compact confocal micro-raman system according to claim 2, wherein the ratio of reflection to transmission of light by said beam splitter is 8: 92.