Fermentation device with dissolved oxygen control and sampling control functions
Technical Field
The utility model relates to a fermentation technical field, more specifically relates to a fermenting installation with dissolved oxygen control and sample control function.
Background
The fermentation tank used at present has the following problems in the fermentation process of facultative anaerobe: oxygen is easily brought in when the fermentation tank is fed with materials, and the oxygen brought in easily causes the dissolved oxygen value in the fermentation tank to be higher, which is extremely unfavorable for bacteria which just start to ferment; with the promotion of the fermentation process, the demand of the bacteria on oxygen begins to increase; since the oxygen demand of the bacteria at different stages of the fermentation process varies, a fermentation device capable of controlling the dissolved oxygen value of the fermentation broth is urgently needed. In addition, in the fermentation process, the bacteria in the tank body need to be sampled and detected in real time so as to know the fermentation condition of the bacteria in the tank body conveniently. The sampling device who uses at present can not extract the sample quantitatively, and draws the back sample and can remain in the sampling tube to the sample that leads to next and testing result appear the deviation, can not accurately embody the real-time fungus in the fermentation cylinder thick.
SUMMERY OF THE UTILITY MODEL
The utility model aims to overcome the defects of the prior art and provide a fermentation device with dissolved oxygen control and sampling control functions.
In order to solve the technical problem, the utility model discloses a technical scheme is: a fermentation device with dissolved oxygen control and sampling control functions comprises a tank body with a sealing structure, an air compressor and a sampling bottle; an oxygen control device for controlling the dissolved oxygen value in the tank body is connected between the tank body and the air compressor through an air pipe; the jar body with be provided with the proportioning device of ration sample through the sampling tube between the sampling bottle.
In the technical scheme, the oxygen control device arranged between the compressor and the tank body can reduce the dissolved oxygen value of fermentation liquor in the tank body when the feeding of strains and serum is finished; along with the promotion of the fermentation process, oxygen can be input into the tank body through the oxygen control device, and the dissolved oxygen value in the tank body is increased so as to meet the requirement of the dissolved oxygen value of the fermentation liquor; the proportioning device who sets up between jar body and the sampling bottle can guarantee the degree of accuracy of sampling volume at every turn, and the proportioning device can also be with remaining the sample transport return jar body in the sampling tube simultaneously, has guaranteed that the testing result of sampling at every turn can accurately embody the internal real-time fermentation condition of jar.
Preferably, the quantitative device comprises a quantitative ring, a first switch valve arranged on a liquid inlet of the quantitative ring and an air inlet branch pipe communicated with the quantitative ring, a second switch valve communicated or closed with the outside air is arranged on the air inlet branch pipe, and a third switch valve is arranged on the quantitative ring and between the air inlet branch pipe and a liquid outlet of the quantitative ring.
In the technical scheme, in the sampling process, the first switch valve and the third switch valve are opened, and the second switch valve is closed; because the air pressure in the sampling bottle is less than the air pressure in the tank body, a sample enters the quantitative ring through the sampling tube, when the sample flows out of the liquid outlet of the quantitative ring, the second switch valve is opened after the first switch valve is closed, so that the sample remained in the quantitative ring flows into the sampling bottle, after the sampling is finished, the third switch valve is closed, the first switch valve is opened, and because the external air pressure is greater than the air pressure in the tank body, the sample remained in the sampling tube is smoothly conveyed back to the tank body.
Preferably, the air inlet branch pipe is communicated with the liquid inlet of the quantitative ring and is positioned below the first switch valve; the third switch valve is arranged at the liquid outlet of the quantitative ring. In this technical scheme, set up like this and be convenient for guarantee the accuracy of sample volume better.
Preferably, the liquid inlet of the quantitative ring is communicated with the sampling tube, and the liquid outlet of the quantitative ring is inserted into the sampling bottle. In this technical scheme, the liquid outlet of ration ring inserts to the sampling bottle in, is convenient for observe the moment that the liquid outlet of ration ring just begins to go out liquid at the in-process of sample.
Preferably, an air filter is arranged on the air inlet branch pipe. In this technical scheme, air cleaner's being provided with does benefit to the impurity of detaching in the air, prevents that impurity from causing the destruction to the sample to influence the accuracy of testing result.
Preferably, the oxygen control device comprises a first pipeline and a second pipeline which are respectively communicated with the air pipe, the first pipeline is provided with a first flow control valve and an oxygen removal device, and the second pipeline is provided with a second flow control valve. In the technical scheme, the oxygen removing device can remove oxygen input into the tank body so as to achieve the purpose of reducing the dissolved oxygen value of the fermentation liquor in the tank body. The first flow control valve and the second flow control valve are arranged to control the flow rate of the gas input into the tank body according to conditions.
Preferably, the air inlets of the first pipeline and the second pipeline are communicated with the air pipe through a three-way joint; and the air outlets of the first pipeline and the second pipeline are communicated with the air pipe through a three-way joint.
Preferably, an oxygen dissolving instrument for detecting the dissolved oxygen value is arranged in the tank body. In the technical scheme, the dissolved oxygen value in the tank body can be controlled in real time by the arrangement of the dissolved oxygen meter, so that the oxygen control device can be accurately operated, and the dissolved oxygen value of the fermentation liquor in the fermentation tank can meet the requirement.
Preferably, a motor is arranged on the tank body, and an output shaft of the motor is provided with a stirring plate. In this technical scheme, the setting of stirring board is in order to make the fermentation more quick, balanced.
Preferably, the sampling port of the sampling tube extends into the bottom of the tank body. In this technical scheme, set up like this and be convenient for can gain the sample of jar body bottom.
Preferably, the air outlet of the air pipe extends into the bottom of the tank body. The arrangement can ensure that the introduced gas is dissolved into the fermentation liquor and the gas drives the fermentation liquor to roll.
Preferably, the dosing ring is of helical configuration. In this technical scheme, the ration ring is helical structure, can be so that at the sample in-process, slow down the speed that the sample flowed into in the ration ring to be convenient for have the first ooff valve of in time closing in the twinkling of an eye that the sample flows out at the liquid outlet of ration ring, guaranteed the accuracy of sample.
Compared with the prior art, the beneficial effects of the utility model are that:
the utility model can adjust the dissolved oxygen value of the fermentation liquor in the tank body in time according to the fermentation condition so as to meet the requirement of controlling the dissolved oxygen value of the fermentation liquor; the accuracy of sampling volume at every turn can be guaranteed in proportioning device's setting, can deliver to the sampling bottle with the sample in the ration ring, and the jar body is sent back to the fungus liquid of remaining in the sampling tube when the sample finishes to guarantee that the testing result of sampling at every turn can accurately embody the real-time fermentation condition in the fermentation cylinder.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Detailed Description
The present invention will be further described with reference to the following embodiments. Wherein the showings are for the purpose of illustration only and are shown by way of illustration only and not in actual form, and are not to be construed as limiting the present patent; for a better understanding of the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar parts; in the description of the present invention, it should be understood that if there are the terms "upper", "lower", "left", "right", etc. indicating the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, it is only for convenience of description and simplification of the description, but it is not intended to indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore the terms describing the positional relationship in the drawings are only for illustrative purposes and are not to be construed as limitations of the present patent, and those skilled in the art can understand the specific meanings of the terms according to specific situations.
Examples
Fig. 1 is a first embodiment of a fermentation device with dissolved oxygen control and sampling control functions, which comprises a tank body 1 with a sealed structure, an air compressor 2 and a sampling bottle 3; an oxygen control device 5 for controlling the dissolved oxygen value of the fermentation liquor in the tank body is connected between the tank body 1 and the air compressor 2 through an air pipe 4; a quantitative device 7 for quantitative sampling is arranged between the tank body 1 and the sampling bottle 3 through a sampling tube 6. An oxygen control device 5 arranged between the air compressor 2 and the tank body 1 can reduce the dissolved oxygen value of the tank body 1 when the feeding of strains and serum is finished; along with the promotion of the fermentation process, oxygen can be input into the tank body 1 through the oxygen control device 5, and the dissolved oxygen value in the tank body 1 is increased, so as to meet the requirement of the dissolved oxygen value of the fermentation liquor; the proportioning device 7 that sets up between jar body 1 and sampling bottle 3 can guarantee the degree of accuracy of sampling volume at every turn, and proportioning device 7 can also carry the sample that remains in sampling tube 6 simultaneously and can ferment body 1, has guaranteed that the testing result of sampling at every turn can accurately embody the fermentation condition in jar body 1.
The quantitative device 7 comprises a quantitative ring 71, a first switch valve 72 arranged on a liquid inlet of the quantitative ring 71, and an air inlet branch pipe 73 communicated with the liquid inlet of the quantitative ring 71, wherein a second switch valve 8 communicated or closed with the outside air is arranged on the air inlet branch pipe 73, and a third switch valve 9 is arranged on a liquid outlet of the quantitative ring 71. In the present embodiment, the quantitative ring 71 is a pipe structure with a certain volume, and the quantitative ring 7 can take a certain volume of sample when the quantitative ring 71 is filled with the sample through the first switch valve 72, the third switch valve 9 and the second switch valve 8 on the matching air inlet branch pipe 73.
In addition, the liquid inlet of the quantitative ring 71 is communicated with the sampling tube 6, and the liquid outlet of the quantitative ring 71 is inserted into the sampling bottle 3. The liquid outlet of the quantitative ring 71 is inserted into the sampling bottle 3, so that the instant when the liquid outlet of the quantitative ring 71 begins to discharge liquid can be observed conveniently in the sampling process.
The intake manifold 73 is provided with an air cleaner 10. The air filter 10 is arranged to remove impurities in the air, so that the impurities are prevented from damaging the sample, and the accuracy of the detection result is affected.
The oxygen controller 5 includes a first pipe 51 and a second pipe 52 communicating with the gas pipe 4, respectively, the first pipe 51 is provided with a first flow rate control valve 11 and an oxygen remover 12, and the second pipe 52 is provided with a second flow rate control valve 13. The oxygen removing device 5 can remove oxygen input into the tank body 1 so as to achieve the purpose of reducing the dissolved oxygen value in the tank body 1. The first flow control valve 11 and the second flow control valve 13 are provided to control the flow rate of the gas supplied to the tank 1 according to circumstances.
Wherein, the air inlets of the first pipeline 51 and the second pipeline 52 are communicated with the air pipe 4 through a three-way joint 17; the air outlets of the first pipeline 51 and the second pipeline 52 are communicated with the air pipe 4 through a three-way joint 17.
In addition, a dissolved oxygen meter 14 for detecting the dissolved oxygen value of the fermentation broth is provided in the tank 1. The oxygen dissolving instrument 14 can control the dissolved oxygen value of the fermentation liquor in the tank body 1 in real time so as to accurately operate the oxygen control device 5, thereby ensuring that the dissolved oxygen value of the fermentation liquor in the fermentation tank meets the requirement. The tank body 1 is provided with a motor 15, and an output shaft of the motor 15 is provided with a stirring plate 16. The stirring plate 16 is provided to make the fermentation faster and more uniform.
Wherein, the sampling port of the sampling tube 6 extends into the bottom of the tank body 1. This arrangement facilitates access to the sample at the bottom of the tank 1. The dosing ring 71 has a helical structure. The quantitative ring 71 is of a spiral structure, so that the speed of the sample flowing into the quantitative ring 71 is reduced in the sampling process, the first switch valve 72 is conveniently closed in time at the moment that the sample flows out of the liquid outlet of the quantitative ring 71, and the sampling accuracy is ensured. The air outlet of the air pipe 4 extends into the bottom of the tank body 1, so that the introduced air can be dissolved into the fermentation liquor, and the air drives the fermentation liquor to roll.
The specific implementation process comprises the following steps:
and (3) dissolved oxygen control process: and starting the air compressor, closing the second flow control valve 13, and opening the first flow control valve 11, so that oxygen in the air input into the tank body 1 by the air compressor 2 is removed by the oxygen removal device 12, and strains and serum are fed, thereby achieving the purpose of reducing the dissolved oxygen value in the tank body 1. After fermentation is started, the second flow control valve 13 is opened, air containing oxygen is input into the tank body 1, the first flow control valve 11 and the second flow control valve 13 are set according to the monitored real-time dissolved oxygen value of the fermentation liquor, and the flow rate of the input air into the tank body 1 is controlled, so that the dissolved oxygen value of the fermentation liquor meets the requirement. Along with the promotion of the fermentation process, the demand of the bacteria in the tank body 1 on the oxygen content is increased, and when the demand is needed, the first flow control valve 11 can be closed to increase the dissolved oxygen value in the tank body 1.
During sampling, the first switch valve 72 and the third switch valve 9 are opened, and the second switch valve 8 is closed; because the air pressure in the sampling bottle 3 is less than the air pressure in the tank body 1, the sample enters the quantitative ring 71 through the sampling tube 6; when a sample flows out from the liquid outlet of the quantitative ring 71, the second switch valve 8 is opened after the first switch valve 72 is closed, so that the sample remained in the quantitative ring 71 flows into the sampling bottle 3; after the sampling is finished, the third switch valve 9 is closed, the first switch valve 72 is opened, and the second switch valve 8 is communicated with the outside, so that the outside air pressure is greater than the air pressure in the tank body 1, and the sample remained in the sampling tube 6 is smoothly conveyed back to the tank body 1.
It is obvious that the above embodiments of the present invention are only examples for clearly illustrating the present invention, and are not limitations to the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.