CN209797981U - Microbial fermentation system - Google Patents
Microbial fermentation system Download PDFInfo
- Publication number
- CN209797981U CN209797981U CN201920518553.4U CN201920518553U CN209797981U CN 209797981 U CN209797981 U CN 209797981U CN 201920518553 U CN201920518553 U CN 201920518553U CN 209797981 U CN209797981 U CN 209797981U
- Authority
- CN
- China
- Prior art keywords
- tank
- tank body
- inlet pipe
- pipe
- seed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
the utility model relates to a microbial fermentation system, which comprises a seed tank, a fermentation tank, an adjusting device and a monitoring device, wherein the seed tank, the fermentation tank and the adjusting device are connected through pipelines; the seed tank comprises a first-stage seed tank and a second-stage seed tank; the fermentation tank comprises a tank body and a tank cover, an outer tank is arranged outside the tank body, and a temperature control cavity is formed between the tank body and the outer tank; the upper part of the tank body is provided with a feeding pipe communicated with the inside of the tank body and an air inlet pipe extending to the bottom of the tank body, and the bottom of the tank body is provided with a discharge hole; the tank cover is provided with a motor, a defoamer, an acid-base liquid inlet pipe and an exhaust pipe; the lower part of the outer tank is provided with a medium inlet pipe and a medium outlet pipe which are communicated with the temperature control cavity; the internal agitating unit that is equipped with of jar, agitating unit upper end and the motor output shaft fixed connection on the cover. The microbial fermentation system integrates the process technology, so that the culture medium and the fermentation liquid are fully utilized, the defects of fungus mass deposition, wall attachment and the like are overcome, and the yield of the fermentation product is obviously improved.
Description
Technical Field
The utility model relates to a microbial fermentation system.
Background
the general process of the existing microbial fermentation engineering comprises the following steps: directional variation induction and alternate rejuvenation to form first-stage seeds, then discharging, transferring the first-stage seeds into a large tank for amplification culture to form large-quantity second-stage seeds, and transferring the second-stage seeds into a fermentation tank for fermentation to form a product. The fermentation process carried out by the seed separation process has a plurality of problems, such as the introduction of exogenous strains in the seed transfer process; the first and second seeds are cultured in the same tank, resulting in small variation, incomplete utilization of culture medium, colony deposition, etc. In addition, the existing fermentation tank has the condition that radial force is formed by stirring to centrifugally throw fungi to the vicinity of the tank wall, so that fermentation liquor cannot be fully utilized, and the yield is influenced by small output of fermentation products, incomplete fermentation and the like.
SUMMERY OF THE UTILITY MODEL
the technical problem to be solved by the utility model is how to overcome the technical defects and integrate the process steps, and a microbial fermentation system is provided.
In order to solve the technical problem, the microbial fermentation system comprises a seed tank, a fermentation tank, an adjusting device and a monitoring device, wherein the seed tank, the fermentation tank and the adjusting device are connected through pipelines; wherein, the seed tank comprises a plurality of first-stage seed tanks for directional variation induction and alternate generation rejuvenation and a second-stage seed tank for expanded propagation culture; the fermentation tank comprises a tank body and a tank cover, an outer tank is arranged outside the tank body, and a temperature control cavity is formed between the tank body and the outer tank; the upper part of the tank body is provided with a feeding pipe communicated with the inside of the tank body and an air inlet pipe extending to the bottom of the tank body, and the bottom of the tank body is provided with a discharge hole; the tank cover is provided with a motor, a defoamer, an acid-base liquid inlet pipe and an exhaust pipe; the lower part of the outer tank is provided with a medium inlet pipe and a medium outlet pipe which are communicated with the temperature control cavity; a stirring device is arranged in the tank body, and the upper end of the stirring device is fixedly connected with a motor output shaft on the tank cover; the adjusting device comprises a heat exchange medium storage tank and an acid-base tank; the monitoring and adjusting mechanism comprises an induction component arranged in the tank body and a display screen arranged outside the tank body. And pump valve mechanisms are arranged on the pipelines and are used for controlling the starting and stopping of the conveying of the bacteria liquid and providing conveying power.
Preferably, the stirring device comprises a rotating shaft and a plurality of stirring shafts arranged on the rotating shaft in a staggered mode, and stirring paddles and flow guide paddles are arranged on the stirring shafts. The stirring paddles are arranged on the upper side and the lower side of the stirring shaft in parallel, and the flow guide paddles are arranged at the end part of the stirring shaft; the stirring paddle is provided with cutting fins, and an included angle between the flow guide paddle and the stirring paddle is smaller than 90 degrees. So design, the fermentation in-process uses two oar formulas stirring, can be on the stirring rake with higher speed dissolved oxygen's basis, shovels back the effect through the guiding paddle, leads radial centrifugal force to be circumferential vortex to overcome the fungus group and piled up at jar external layer, the unable make full use of zymotic fluid, lead to the defect that fermentation product volume is low, the rising bubble can further be broken into pieces to the cutting wing on the guiding paddle, further improves the dissolved oxygen effect in the zymotic fluid.
Preferably, the end part of the air inlet pipe is an arc-shaped blind end, and a section of the air inlet pipe, which is positioned at the arc-shaped blind end, is provided with a large number of air outlet holes. By the design, the pumped sterile oxygen can be distributed more uniformly and bubbles can be smaller, so that the dissolved oxygen density of each region in the fermentation tank is balanced, the utilization rate of thalli is improved, and the fermentation efficiency is improved.
Preferably, an air filter is connected to the outside of the air inlet pipe. So design, air cleaner is the aseptic air cleaner of PP melt-blown filter core stainless steel of Yuwang machinery manufacturing Limited company production in Yuzhou city, can ally oneself with ordinary air extractor or air compressor machine outward and carry out the preceding stage degerming of single filter core reverse osmosis, avoids the introduction of the gaseous a large amount of exogenous microorganisms of entering.
As optimization, the first-stage seed tank is a conical bottom tank, and a lifting auger is arranged in the second-stage seed tank. By the design, the large-diameter first-stage seeding tank with the conical bottom can fully spread the directed mutagenesis culture medium on an oxygen contact surface, and is favorable for directed variation induction and alternate rejuvenation of primary microorganisms; the secondary seed tank enables the bacterial quantity and the concentration of the culture medium to be balanced in the upper part and the lower part of the pipe under the action of continuously lifting and overturning the auger, and avoids the phenomenon of bacterial colony type concentration in the proliferation process.
Preferably, the sensing assembly comprises a temperature sensor, a pH sensor and a dissolved oxygen sensor. So design, pH sensor, temperature sensor, dissolved oxygen sensor all use the current abluent probe form sensor of being convenient for (temperature probe, pH probe, dissolved oxygen probe), and the probe is connected with the outside LCD display screen that is used for showing the registration.
as optimization, the first-stage seed tank and the second-stage seed tank are connected through a seed transferring pipeline, and the second-stage seed tank is connected with a feeding pipe through a feeding pipeline; the heat exchange medium storage tank is connected with the medium inlet pipe and the medium outlet pipe through the temperature control pipe; the acid-base tank is connected with the acid-base liquid inlet pipe through an adjusting pipe.
The design is that the ingredient strain is delivered to a second-stage seed tank filled with the ingredient strain in a directional mutagenesis culture medium paved in a conical bottom of a first-stage seed tank to complete directional variation induction and alternate rejuvenation, the ingredient strain is transferred to the second-stage seed tank for amplification culture by a seed transplanting pipe, the bacterium amount and the concentration of the culture medium are balanced at the upper part and the lower part in the seed tank under the action of continuous lifting and overturning of a packing auger, the phenomenon of bacterium cluster type concentration in the proliferation process is avoided, the proliferated bacterium solution is delivered to a fermentation tank for fermentation, condition parameters such as the temperature, the pH value, the dissolved oxygen concentration and the like in the tank are monitored by a monitoring device, water or oil is introduced into a medium inlet pipe and a medium outlet pipe to perform heat exchange in the tank by taking the water or the oil as a cold and hot medium, the fermentation temperature is controlled, acid-alkali solution is introduced into the acid-alkali tank to perform pH adjustment, the double-paddle type stirring is performed, thereby overcome the fungus group and piled up at the external layer of jar, the fermented liquid can't make full use of, leads to the defect that fermentation product volume is low, and the cutting wing on the water conservancy diversion oar can further smash the rising bubble, further improves the dissolved oxygen effect in the fermented liquid.
The utility model relates to a microbial fermentation system has integrated flow process, makes culture medium and zymotic fluid obtain make full use of to overcome defects such as fungus group deposit, attaches the wall, and then show the productivity that has improved the fermentation product.
Drawings
The following description of the microbial fermentation system of the present invention is made with reference to the accompanying drawings:
FIG. 1 is a schematic plan view of the present microbial fermentation system;
FIG. 2 is a schematic view of the internal structure of the present microbial fermentation system;
FIG. 3 is a partial enlarged view of FIG. 2 (enlarged in the area A of FIG. 2);
FIG. 4 is a perspective view of a fermenter of the present microorganism fermentation system;
FIG. 5 is a schematic plan view of a fermenter of the present microorganism fermentation system;
FIG. 6 is a schematic sectional view taken along line A-A of FIG. 5;
FIG. 7 is a schematic top view of the stirring device of the microbial fermentation system.
In the figure:
1-a seed tank, 2-a fermentation tank, 3-an adjusting device, 4-a monitoring device, 5-a pipeline and 6-a stirring device;
11-a first-stage seed tank, 12-a second-stage seed tank, 21-a tank body, 22-a tank cover, 23-an outer tank, 31-a heat exchange medium storage tank, 32-an acid-base tank, 41-a temperature sensor, 42-a pH sensor, 43-a dissolved oxygen sensor, 51-a seed transferring pipeline, 52-a feeding pipeline, 53-a temperature control pipe, 54-an adjusting pipe, 61-a rotating shaft and 62-a stirring shaft;
123-lifting auger, 203-temperature control cavity, 211-feeding pipe, 212-air inlet pipe, 213-discharging port, 221-motor, 222-defoamer, 223-acid-alkali liquid inlet pipe, 224-exhaust pipe, 231-medium inlet pipe, 232-medium outlet pipe, 621-stirring paddle, 622-flow guide paddle and 623-cutting fin;
2121-air outlet, 2122-air filter.
Detailed Description
as shown in fig. 1 to 7, the microbial fermentation system comprises a seed tank 1, a fermentation tank 2, an adjusting device 3 and a monitoring device 4, wherein the seed tank 1, the fermentation tank 2 and the adjusting device 3 are connected through a pipeline 5; wherein, the seed tank 1 comprises a plurality of first-stage seed tanks 11 for directional variation induction and alternate generation rejuvenation and a second-stage seed tank 12 for expanded propagation culture; the fermentation tank 2 comprises a tank body 21 and a tank cover 22, an outer tank 23 is arranged outside the tank body 21, and a temperature control cavity 203 is formed between the tank body 21 and the outer tank 23; the upper part of the tank body 21 is provided with a feeding pipe 211 communicated with the inside of the tank body 21 and an air inlet pipe 212 extending to the bottom of the tank body 21, and the bottom of the tank body 21 is provided with a discharging hole 213; the tank cover 22 is provided with a motor 221, a foam breaker 222, an acid and alkali liquid inlet pipe 223 and an exhaust pipe 224; a medium inlet pipe 231 and a medium outlet pipe 232 which are communicated with the temperature control cavity 203 are arranged at the lower part of the outer tank 23; a stirring device 6 is arranged in the tank body 21, and the upper end of the stirring device 6 is fixedly connected with an output shaft of a motor 221 on the tank cover 22; the adjusting device 3 comprises a heat exchange medium storage tank 31 and an acid-base tank 32; the monitoring and adjusting mechanism 4 comprises a sensing component arranged in the tank body 21 and a display screen arranged outside the tank body 21. And the pipeline 5 is provided with a pump valve mechanism for controlling the start and stop of the bacterial liquid conveying and providing conveying power.
the stirring device 6 comprises a rotating shaft 61 and a plurality of stirring shafts 62 arranged on the rotating shaft 61 in a staggered manner, wherein stirring paddles 621 and diversion paddles 622 are arranged on the stirring shafts 62. The stirring paddles 621 are arranged on the upper side and the lower side of the stirring shaft 62 in parallel, and the flow guide paddles 622 are arranged at the end part of the stirring shaft 62; the stirring paddle 621 is provided with cutting fins 623, and an included angle between the flow guide paddle 622 and the stirring paddle 621 is smaller than 90 degrees. The double-paddle stirring is used in the fermentation process, on the basis that the stirring paddle accelerates the oxygen dissolution, the shoveling effect of the flow guiding paddle is used for guiding the radial centrifugal force into the circumferential vortex, so that the defect that the fermentation product amount is low due to the fact that the fungus mass is accumulated on the outer layer of the tank body and the fermentation liquid cannot be fully utilized is overcome, the cutting fins on the flow guiding paddle can further break the rising bubbles, and the oxygen dissolution effect in the fermentation liquid is further improved.
The end of the inlet pipe 212 is an arc-shaped blind end, and a section of the inlet pipe 212 at the arc-shaped blind end is provided with a plurality of air outlet holes 2121. The distribution of the pumped sterile oxygen is more uniform, the bubbles are smaller, so that the dissolved oxygen density in each area in the fermentation tank is improved, the utilization rate of thalli is improved, and the fermentation efficiency is improved.
An air filter 2122 is connected to the outside of the intake pipe 212. The air filter is a PP melt-blown filter element stainless steel sterile air filter produced by Yuwang machinery manufacturing Limited of Yuzhou city, and can be externally connected with a common air pump or an air compressor to perform single-filter-element reverse osmosis pre-stage sterilization, so that the introduction of a large amount of exogenous microorganisms into the intake gas is avoided.
The first-stage seeding tank 11 is a conical bottom tank, and a lifting auger 123 is arranged in the second-stage seeding tank 12. The large-diameter first-stage seeding tank with the conical bottom can fully spread the directed mutagenesis culture medium on an oxygen contact surface, and is favorable for directed mutation induction and alternate rejuvenation of primary microorganisms; the secondary seed tank enables the bacterial quantity and the concentration of the culture medium to be balanced in the upper part and the lower part of the pipe under the action of continuously lifting and overturning the auger, and avoids the phenomenon of bacterial colony type concentration in the proliferation process.
The sensing components include a temperature sensor 41, a pH sensor 42, and a dissolved oxygen sensor 43. The pH sensor, the temperature sensor and the dissolved oxygen sensor all use the existing probe form sensor temperature probe, the pH probe and the dissolved oxygen probe which are convenient to clean, and the probes are connected with an LCD display screen which is used for displaying readings outside.
The first-stage seed tank 11 and the second-stage seed tank 12 are connected through a seed transferring pipeline 51, and the second-stage seed tank 12 is connected with a feeding pipe 211 through a feeding pipeline 52; the heat exchange medium storage tank 31 is connected with a medium inlet pipe 231 and a medium outlet pipe 232 through a temperature control pipe 53; the acid-base tank 32 is connected with an acid-base liquid inlet pipe 223 through a regulating pipe 54.
The method comprises the steps of preparing a strain, spreading the strain in a directional mutagenesis culture medium in a conical bottom of a first-stage seed tank to complete directional variation induction and alternate rejuvenation, conveying the strain to a second-stage seed tank filled with the strain through a seed transplanting pipe for amplification propagation culture, balancing the strain quantity and the concentration of the culture medium at the upper part and the lower part in the pipe under the action of continuous lifting and overturning of an auger to avoid the phenomenon of colony type concentration in the propagation process, conveying the propagated strain liquid into a fermentation tank for fermentation, monitoring the condition parameters such as the temperature, the pH value, the concentration of dissolved oxygen and the like in the tank by using a monitoring device, introducing water or oil into an inlet pipe and a medium outlet pipe as cold and hot media to perform in-tank heat exchange and control the fermentation temperature, introducing acid-alkali liquid into the acid-alkali tank to perform pH adjustment, stirring with double paddles in the process on the basis of accelerating dissolved oxygen by using stirring paddles, and guiding the radial centrifugal force into a circumferential, thereby overcome the fungus group and piled up at the external layer of jar, the fermented liquid can't make full use of, leads to the defect that fermentation product volume is low, and the cutting wing on the water conservancy diversion oar can further smash the rising bubble, further improves the dissolved oxygen effect in the fermented liquid.
The microbial fermentation system integrates the process technology, so that the culture medium and the fermentation liquid are fully utilized, the defects of fungus mass deposition, wall attachment and the like are overcome, and the yield of the fermentation product is obviously improved.
The above embodiments are intended to be illustrative of the manner in which the invention may be made and used by persons skilled in the art, and modifications to the above embodiments will be apparent to those skilled in the art, and it is therefore intended that the invention, including but not limited to the above embodiments, be limited to any methods, processes and products consistent with the principles and novel and inventive features disclosed herein, and which are to be interpreted as illustrative and not in a limiting sense.
Claims (8)
1. A microbial fermentation system is characterized in that: the device comprises a seed tank (1), a fermentation tank (2), an adjusting device (3) and a monitoring device (4), wherein the seed tank (1), the fermentation tank (2) and the adjusting device (3) are connected through a pipeline (5); wherein the content of the first and second substances,
The seeding tank (1) comprises a plurality of first-stage seeding tanks (11) for directional variation induction and alternate generation rejuvenation and second-stage seeding tanks (12) for expanded propagation culture;
The fermentation tank (2) comprises a tank body (21) and a tank cover (22), an outer tank (23) is arranged outside the tank body (21), and a temperature control cavity (203) is formed between the tank body (21) and the outer tank (23); a feeding pipe (211) communicated with the interior of the tank body (21) and an air inlet pipe (212) extending to the bottom of the tank body (21) are arranged at the upper part of the tank body (21), and a discharging hole (213) is arranged at the bottom of the tank body (21); the tank cover (22) is provided with a motor (221), a defoamer (222), an acid and alkali liquid inlet pipe (223) and an exhaust pipe (224); a medium inlet pipe (231) and a medium outlet pipe (232) which are communicated with the temperature control cavity (203) are arranged at the lower part of the outer tank (23); a stirring device (6) is arranged in the tank body (21), and the upper end of the stirring device (6) is fixedly connected with an output shaft of a motor (221) on the tank cover (22);
The adjusting device (3) comprises a heat exchange medium storage tank (31) and an acid-base tank (32); the monitoring device (4) comprises a sensing assembly arranged in the tank body (21) and a display screen arranged outside the tank body (21).
2. The microbial fermentation system of claim 1, wherein: agitating unit (6) are including pivot (61), a plurality of (mixing) shaft (62) of dislocation set on this pivot (61), be equipped with stirring rake (621) and water conservancy diversion oar (622) on (mixing) shaft (62).
3. The microbial fermentation system of claim 2, wherein: the stirring paddles (621) are arranged on the upper side and the lower side of the stirring shaft (62) in parallel, and the flow guide paddles (622) are arranged at the end part of the stirring shaft (62); cutting fins (623) are arranged on the stirring paddle (621), and the included angle between the flow guide paddle (622) and the stirring paddle (621) is smaller than 90 degrees.
4. the microbial fermentation system of claim 3, wherein: the end part of the air inlet pipe (212) is an arc-shaped blind end, and a section of the air inlet pipe (212) positioned at the arc-shaped blind end is provided with a large number of air outlet holes (2121).
5. the microbial fermentation system of claim 4, wherein: an air filter (2122) is connected to the outside of the air inlet pipe (212).
6. The microbial fermentation system of claim 1, wherein: the first-stage seed tank (11) is a conical bottom tank, and a lifting auger (123) is arranged in the second-stage seed tank (12).
7. The microbial fermentation system of claim 3, wherein: the sensing assembly comprises a temperature sensor (41), a pH sensor (42) and a dissolved oxygen sensor (43).
8. The microbial fermentation system of claim 1, wherein: the first-stage seed tank (11) and the second-stage seed tank (12) are connected through a seed transferring pipeline (51), and the second-stage seed tank (12) is connected with a feeding pipe (211) through a feeding pipeline (52); the heat exchange medium storage tank (31) is connected with the medium inlet pipe (231) and the medium outlet pipe (232) through the temperature control pipe (53); the acid-base tank (32) is connected with an acid-base liquid inlet pipe (223) through a regulating pipe (54).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920518553.4U CN209797981U (en) | 2019-04-16 | 2019-04-16 | Microbial fermentation system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920518553.4U CN209797981U (en) | 2019-04-16 | 2019-04-16 | Microbial fermentation system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209797981U true CN209797981U (en) | 2019-12-17 |
Family
ID=68829692
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920518553.4U Expired - Fee Related CN209797981U (en) | 2019-04-16 | 2019-04-16 | Microbial fermentation system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209797981U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112877202A (en) * | 2021-01-18 | 2021-06-01 | 刘永昶 | Automatic monitoring control system of edible and medicinal fungus liquid culture fermentation system |
-
2019
- 2019-04-16 CN CN201920518553.4U patent/CN209797981U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112877202A (en) * | 2021-01-18 | 2021-06-01 | 刘永昶 | Automatic monitoring control system of edible and medicinal fungus liquid culture fermentation system |
| CN112877202B (en) * | 2021-01-18 | 2024-04-16 | 天津山珍科技有限公司 | Automatic monitoring control system of edible and medicinal fungus liquid culture fermentation system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101671625A (en) | Method and device for preparing trichoderma conidium by liquid state submerged fermentation | |
| CN102318789A (en) | Solid-state fermentation equipment and method for preparing red yeast rice using same | |
| CN209797981U (en) | Microbial fermentation system | |
| CN103525689B (en) | High dissolved oxygen bioreactor for high-density culture of genetically engineered bacteria and culture control method | |
| CN110656043A (en) | Microbial fermentation tank for producing liquid feed additive | |
| CN206545023U (en) | Solid microbe production equipment and microbial manure production system | |
| CN210394377U (en) | Anaerobe culture system | |
| CN201834920U (en) | Rotary drum bacterial cellulose fermentation device | |
| CN103865753B (en) | Liquid and solid combined fungicide fermentation and preparation system | |
| CN207313607U (en) | A kind of fermentation tank for promoting bacterial metabolism | |
| CN202127781U (en) | Solid state fermentation equipment | |
| CN105273994B (en) | A kind of synthesis gas anaerobic fermentation tower reactor | |
| CN114134038A (en) | Novel aerobic fermentation device for experiments | |
| CN207079239U (en) | Solid state fermentation system | |
| CN206680491U (en) | Novel microbial installation for fermenting | |
| CN206375894U (en) | Cultivate the second order fermentation system of bacillus thuringiensis | |
| CN208604121U (en) | A kind of spawn fermentation tank | |
| CN208250300U (en) | A kind of ferment strain three-level continuously spreads cultivation device | |
| CN206680474U (en) | Microbial fermentation inlet duct | |
| CN201398323Y (en) | Fermentation device for edible fungi liquid seed production | |
| CN205635621U (en) | Fermentation cylinder convenient to retrieve bacterial | |
| CN214881465U (en) | Fermentation tank | |
| CN205062072U (en) | Yellow fermenting installation of mulberry | |
| CN117256478B (en) | Continuous fermentation culture system for plant tissues | |
| CN109439515B (en) | Rotary ball type microorganism two-stage expanding culture device and method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191217 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |