CN209764680U - Pressure control circuit of fluid module based on flow cytometer - Google Patents
Pressure control circuit of fluid module based on flow cytometer Download PDFInfo
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- CN209764680U CN209764680U CN201920434818.2U CN201920434818U CN209764680U CN 209764680 U CN209764680 U CN 209764680U CN 201920434818 U CN201920434818 U CN 201920434818U CN 209764680 U CN209764680 U CN 209764680U
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Abstract
the utility model discloses a pressure control circuit of fluid module based on flow cytometer, include: the pressure detection circuit comprises a power supply conversion circuit, a pressure acquisition circuit, a pressure detection circuit, a pressure adjustment circuit and a reference signal selection circuit; the pressure acquisition circuit and the pressure adjusting circuit are respectively in communication connection with the input end of the pressure detection circuit; the output end of the pressure detection circuit is in communication connection with the pressure adjusting circuit; when the pressure control circuit is powered by the power supply conversion circuit, the flowing pressure signal of the sheath fluid collected by the pressure collection circuit is input to the pressure detection circuit, compared with the reference pressure signal provided by the reference signal selection circuit and outputs the generated pressure difference, the pressure difference output by the pressure detection circuit is input to the pressure adjustment circuit, and the pressure adjustment circuit adjusts the flow of the sheath fluid flowing through the pressure control valve according to the pressure difference, so that the flowing pressure of the sheath fluid is controlled.
Description
Technical Field
the utility model relates to the field of electronic technology, especially, relate to a fluid module's pressure control circuit based on flow cytometer.
background
At present, a plurality of pressure control circuits are arranged in a fluid module of a flow cytometer, and the control circuits are complex and have poor stability, so that unstable pressure control is easy to generate or the overall cost is high.
therefore, based on the technical problem who exists above, the utility model provides a solve above technical problem's technical scheme.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a pressure control circuit based on fluid module of flow cytometer utilizes pressure sensor to listen actual operation's sheath fluid pressure and converts corresponding voltage into, carries out comparison with reference voltage through the comparator and enlargies the back, and the output transistor control adds the voltage size of pressure control valve, and the pressure control valve can change the aperture size of sheath liquid process under the voltage size of difference to the size of control sheath fluid pressure, the circuit is simple reliable and stable.
The utility model provides a technical scheme as follows:
A pressure control circuit of a flow cytometer based fluidic module, comprising: the pressure detection circuit comprises a power supply conversion circuit, a pressure acquisition circuit, a pressure detection circuit, a pressure adjustment circuit and a reference signal selection circuit; the power supply conversion circuit is respectively in communication connection with the pressure acquisition circuit, the pressure detection circuit, the pressure adjustment circuit and the reference signal selection circuit; the pressure acquisition circuit and the pressure adjusting circuit are respectively in communication connection with the input end of the pressure detection circuit; the output end of the pressure detection circuit is in communication connection with the pressure adjusting circuit; when the pressure control circuit is powered by the power conversion circuit, the flowing pressure signal of the sheath fluid collected by the pressure collection circuit is input to the pressure detection circuit, compared with the reference pressure signal provided by the reference signal selection circuit and outputs the generated pressure difference, the pressure difference output by the pressure detection circuit is input to the pressure adjustment circuit, and the flow of the sheath fluid flowing through the pressure control valve is adjusted by the pressure adjustment circuit according to the pressure difference, so that the flowing pressure of the sheath fluid is controlled.
in the scheme, the pressure sensor is used for detecting the pressure of sheath fluid in actual operation and converting the pressure into a corresponding voltage signal, the output transistor controls and loads the voltage signal to the pressure adjusting circuit after the voltage signal is compared and amplified with the reference voltage through the comparator, the voltage of the pressure control valve is controlled, the pressure control valve can change the size of the aperture through which the sheath fluid passes under different voltage sizes, the size of the pressure of the sheath fluid is controlled, and the circuit is simple, stable and reliable.
Further preferably, the pressure acquisition circuit includes: pressure sensors, R1, R2, C5, C6; the signal detection ends of the pressure sensors are respectively connected with one ends of R1 and R2 in a communication way; the other end of R2 is grounded; the other end of the R1 is connected with the pressure detection circuit in a communication way; the signal detection end of the pressure sensor is also in communication connection with one ends of C5 and C6, and the other ends of C5 and C6 are grounded.
Further preferably, the pressure detection circuit includes: a comparison amplifier; the reverse input end of the comparison amplifier is in communication connection with the other end of the R1 in the pressure acquisition circuit; the positive input end of the comparison amplifier is in communication connection with the reference signal selection circuit; the output end of the comparison amplifier is connected with the pressure adjusting circuit in a communication mode.
Further preferably, the reference signal selection circuit includes: an analog voltage gating chip, R3, R4, R5, VR1, VR2, VR 3; one end of R3 is connected with a power supply, the other end of R3 is connected with one end of VR1, and the other end of VR1 is grounded; the common connection end of the R3 and the VR1 is in communication connection with the first signal gating end of the analog voltage gating chip; the common connection end of the R4 and the VR2 is in communication connection with the second signal gating end of the analog voltage gating chip; the common connection end of the R5 and the VR3 is in communication connection with the third signal gating end of the analog voltage gating chip; and the communication end of the analog voltage gating chip is in communication connection with the positive input end of the comparison amplifier in the pressure detection circuit.
Further preferably, the system also comprises a central processing unit; and the central processing unit is respectively in communication connection with the signal switching control end of the analog voltage gating chip in the reference signal selection circuit.
Further preferably, the pressure adjustment circuit includes: a switching tube and a pressure control valve; the collector end of the switching tube is in communication connection with the output end of a comparison amplifier in the pressure detection circuit; the collector terminal of the switching tube is in communication connection with the power conversion circuit; the emitter end of the switching tube is in communication connection with the power supply end of the pressure control valve; the ground end of the pressure control valve is grounded.
further preferably, the power conversion circuit includes: the first voltage stabilizing chip and the second voltage stabilizing chip are connected in series; the power supply input end of the first voltage stabilizing chip is in communication connection with a power supply end, and the voltage output end of the first voltage stabilizing chip outputs a first power supply voltage; the power supply input end of the second voltage stabilizing chip is in communication connection with the power supply end, and the voltage output end of the second voltage stabilizing chip outputs a second power supply voltage.
The utility model provides a pair of fluid module's pressure control circuit based on flow cytometer brings following beneficial effect at least as follows:
the utility model discloses in, utilize pressure sensor to carry out pressure voltage signal conversion, then set for the voltage signal that 3 kinds of velocity of flow correspond as reference voltage, the voltage size of opening the control supply pressure control valve by the triode after amplifier carries out comparative amplification, thereby control sheath liquid and flow through the pressure that the aperture size of pressure control valve controlled the sheath liquid, 3 kinds of velocity of flow that we chose are controlled through MCU's GPIO, complicated control circuit and unstable control ring have been avoided, let the pressure control of fluid cell test more stable, and good cost-effectiveness.
Drawings
The foregoing features, technical features, and advantages of a flow cytometer based pressure control circuit for a fluidic module and implementations thereof will be further described in the following detailed description of preferred embodiments in conjunction with the accompanying drawings.
fig. 1 is a schematic diagram of an embodiment of a pressure control circuit of a flow cytometer based fluidic module according to the present invention;
fig. 2 is a circuit diagram of the pressure acquisition circuit of the present invention;
Fig. 3 is a circuit diagram of the pressure detection circuit of the present invention;
Fig. 4 is a circuit diagram of the reference signal selection circuit of the present invention;
Fig. 5 is a circuit diagram of the pressure regulating circuit of the present invention;
Fig. 6 is a circuit diagram of the power conversion circuit diagram of the present invention.
The symbols of the attached drawings: 100. the pressure detection circuit comprises a power supply conversion circuit, a 200 pressure acquisition circuit, a 300 pressure detection circuit, a 400 pressure adjustment circuit, a 500 reference signal selection circuit and a 600 central processing unit.
Detailed Description
In order to more clearly illustrate embodiments of the present invention or technical solutions in the prior art, specific embodiments of the present invention will be described below with reference to the accompanying drawings. It is obvious that the drawings in the following description are only examples of the invention, and that for a person skilled in the art, other drawings and embodiments can be obtained from these drawings without inventive effort.
For the sake of simplicity, the drawings only schematically show the parts relevant to the present invention, and they do not represent the actual structure of the product.
Referring to fig. 1, a schematic diagram of an embodiment of a pressure control circuit of a flow cytometer based fluid module is provided; the pressure control circuit of the fluid module of the flow cytometer comprises: the pressure detection circuit comprises a power supply conversion circuit 100, a pressure acquisition circuit 200, a pressure detection circuit 300, a pressure adjustment circuit 400 and a reference signal selection circuit 500; the power conversion circuit 100 is respectively connected with the pressure acquisition circuit 200, the pressure detection circuit 300, the pressure adjustment circuit 400 and the reference signal selection circuit 500 in a communication manner; the pressure acquisition circuit 200 and the pressure adjusting circuit 400 are respectively in communication connection with the input end of the pressure detection circuit 300; the output end of the pressure detection circuit 300 is in communication connection with the pressure adjustment circuit 400; after the pressure control circuit is powered by the power conversion circuit, the flowing pressure signal of the sheath fluid collected by the pressure collection circuit is input to the pressure detection circuit, compared with the reference pressure signal provided by the reference signal selection circuit 500, and the generated pressure difference is output, the pressure difference output by the pressure detection circuit 300 is input to the pressure adjustment circuit, and the flow of the sheath fluid flowing through the pressure control valve is adjusted by the pressure adjustment circuit 400 according to the magnitude of the pressure difference, so that the flowing pressure of the sheath fluid is controlled.
specifically, in the present application, the power conversion circuit provides two voltages, which convert the input 15V voltage into 12V and 5V; respectively and normally supplying power to each corresponding circuit of 12V and 5V; the pressure sensor is used for detecting the pressure of sheath fluid in actual operation, the sheath fluid is converted into a corresponding voltage signal, after the sheath fluid is compared and amplified with reference voltage through the comparator, the output transistor is controlled and loaded to the pressure adjusting circuit, the voltage of the pressure control valve is controlled, the pressure control valve can change the size of the aperture through which the sheath fluid passes under different voltage sizes, the size of the sheath fluid pressure is controlled, and the circuit is simple, stable and reliable.
Referring to fig. 2, the present invention provides a pressure acquisition circuit of a pressure control circuit of a flow cytometer-based fluid module, comprising: pressure sensors U1, R1, R2, C5, C6; the signal detection end (V _ Sense) of the pressure sensor is respectively connected with one ends of R1 and R2 in a communication way; the other end of R2 is grounded; meanwhile, the divider resistors R1 and R2 are used for reducing the induction voltage to a proper value in proportion and inputting the reduced induction voltage to a comparator of the pressure detection circuit; the signal detection end (V _ Sense) of the pressure sensor is also connected with one end of C5 and C6 in a communication way, and the other ends of C5 and C6 are grounded; the filter capacitor C5 and the pressure conversion voltage of the C6 sensor perform a filtering function. The pressure acquisition circuit realizes the real-time acquisition of the flowing signal of the sheath fluid, and can realize the real-time monitoring and acquisition.
referring to fig. 3, the present invention provides a pressure detection circuit of a pressure control circuit of a flow cytometer-based fluid module, comprising: a comparison amplifier; the reverse input end of the comparison amplifier is in communication connection with the other end of the R1 in the pressure acquisition circuit; the positive input end of the comparison amplifier is in communication connection with the reference signal selection circuit; the output of the comparison amplifier is communicatively coupled to the pressure adjustment circuit.
Specifically, in the present application, referring to fig. 3, an operational amplifier U4A, a filter capacitor C11, a feedback capacitor C12, a feedback resistor R6; the output end of the operational amplifier U4A is connected with the pressure adjusting circuit 400; the positive input end of the operational amplifier U4A is connected with the output end Vcom of the analog signal gating chip of the reference signal selection circuit 500, and the negative input end of the operational amplifier U4A is connected with the output end of the pressure sensor output of the pressure sensor conversion circuit after voltage division by R1 and R2; one end of the filter capacitor C11 is connected with the VCC power supply end of the amplifier chip U4A, the second end is connected with the ground, one end of the feedback capacitor C12 is connected with the negative input end of the operational amplifier U4A, the second end of the feedback capacitor C12 is connected with the feedback resistor R6, one end of the feedback resistor R6 is connected with the output end of the operational amplifier U4A, and the second end of the feedback capacitor C12 is connected with the R6. The pressure detection circuit realizes the detection of the flowing pressure signal of the sheath fluid, in the application, reference signals are set to be 3, the reference signals are respectively compared with the flowing pressure signal of the detected sheath fluid according to three grades of high, medium and low voltage signals of the voltage value, and the difference is amplified by the comparator and then fed back to the pressure adjusting circuit.
Referring to fig. 4, the present invention provides a reference signal selection circuit of a pressure control circuit of a flow cytometer-based fluid module, comprising: the device comprises an analog voltage gating chip U5, R3, R4, R5, VR1, VR2 and VR3, wherein one end of the R3 is connected with a power supply, the other end of the R3 is connected with one end of the VR1, and the other end of the VR1 is grounded; the common connection of the R3 and the VR1 is communicatively connected to a first signal gating terminal (NO1) of the analog voltage gating chip, which is a high voltage reference signal, e.g., 5V; the common connection terminal of R4 and VR2 is communicatively connected to the second signal gating terminal (NO2) of the analog voltage gating chip, between the mid-range voltage reference signal, e.g., 4V; (ii) a The common connection terminal of R5 and VR3 is communicatively connected to the third signal gating terminal (NO3) of the analog voltage gating chip, which is a low voltage reference signal, e.g., 2.5V; and a communication terminal (COM) of the analog voltage gating chip is in communication connection with a positive input terminal of a comparison amplifier in the pressure detection circuit.
preferably, the present invention provides a pressure control circuit of a flow cytometer-based fluid module, further comprising a central processing unit 600; the central processing unit (MCU chip) is respectively connected with the signal switching control ends (IN1 and IN2) of the analog voltage gating chip IN the reference signal selection circuit IN a communication way.
specifically, R3, R4, R5 are voltage dividing resistors; VR1, VR2 and VR3 divide adjustable resistors, adjust according to the size of signals, filter capacitor C10 and MCU chip UX; the voltage dividing resistor R3 and the voltage dividing adjustable resistor VR1 are used for dividing voltage of 15V to set reference voltage of corresponding pressure of the sheath liquid at a high flow rate and are connected to a gating chip U5, the voltage dividing resistor R4 and the voltage dividing adjustable resistor VR2 are used for dividing voltage of 15V to set reference voltage of corresponding pressure of the sheath liquid at a medium flow rate and are connected to a gating chip U5, the voltage dividing resistor R5 and the voltage dividing adjustable resistor VR3 are used for dividing voltage of 15V to set reference voltage of corresponding pressure of the sheath liquid at a low flow rate and are connected to a gating chip U5, the filter capacitor C10 is used for supplying power to the gating chip VCC and is connected to the gating chip U5 through an aluminum foil, the MCU chip provides a gating signal and is connected to the gating chip U5, and reference voltage Vcom output by the gating chip is connected. Therefore, the central processing unit and the signal selection circuit of the application realize the adjustment of the size of the reference voltage; the switching of the signals is realized through the central processing unit; the signal switching is realized by switching high, middle and low signals.
Referring to fig. 5, the present invention provides a pressure adjustment circuit of a pressure control circuit of a flow cytometer-based fluid module, comprising: a pressure control valve VSQ1, a voltage regulation output triode Q1, a voltage overshoot prevention diode D2; a transistor Q1 for adjusting the voltage supplied to the pressure control valve, and a diode D2 for suppressing the voltage applied to the pressure control valve to a maximum of 12V + VD (diode forward conduction voltage); the VCC of the pressure control valve VSQ1 is connected to the emitter of the voltage regulation output triode Q1, and the GND terminal of the pressure control valve VSQ1 is connected to the ground; the base electrode of the voltage regulation output triode Q1 is connected to the output end of the amplifier, and the emitter electrode of the voltage regulation output triode Q1 is connected to VCC of the pressure control valve VSQ 1; the positive electrode of the overshoot voltage diode D2 is connected to the collector of the voltage regulation output triode Q1, and the negative electrode of the overshoot voltage diode D2 is connected to the output end 12V of the linear power supply U2; the overshoot voltage diode D2 is used to suppress a transient spike applied to the control valve when the voltage applied to the pressure control valve changes, and to make the voltage at most 12V + VD (diode conduction voltage) and suppress overshoot.
Referring to fig. 2, the present invention provides a power conversion circuit diagram of a pressure control circuit of a flow cytometer-based fluid module, including: the power supply is supplied to the 12V pressure control valve and the 5V gating chip, the linear voltage-stabilizing chip U2 (a first voltage-stabilizing chip) and the filter capacitor C2; a filter capacitor C3; a filter capacitor C4, a filter inductor L1, a linear voltage stabilization chip U3 (a second voltage stabilization chip), a filter capacitor C7; a filter capacitor C8; a filter capacitor C9, a filter inductor L2; the output 12V of the linear voltage stabilizing chip U2 is connected to a collector of a Q1 of a pressure control valve supply power circuit, the output 5V of the linear voltage stabilizing chip U3 is connected to a gating chip, the filtering capacitor C2 wire connection voltage stabilizing power chip U2, the filtering capacitor C7 wire connection voltage stabilizing power chip U3 is connected with the second end of the linear voltage stabilizing chip U3 to be grounded, the C3, the L1 and the C4 form pi-type filter wire connecting power chip U2 output, and the C8, the L2 and the C9 form pi-type filter wire connecting power chip U3 output and are used for removing high-frequency ripples and noises.
the utility model discloses in, utilize pressure sensor to carry out pressure voltage signal conversion, then set for the voltage signal that 3 kinds of velocity of flow correspond as reference voltage, the voltage size of opening the control supply pressure control valve by the triode after amplifier carries out comparative amplification, thereby control sheath liquid and flow through the pressure that the aperture size of pressure control valve controlled the sheath liquid, 3 kinds of velocity of flow that we chose are controlled through MCU's GPIO, complicated control circuit and unstable control ring have been avoided, let the pressure control of fluid cell test more stable, and good cost-effectiveness.
it should be noted that the above embodiments can be freely combined as necessary. The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and decorations can be made without departing from the principle of the present invention, and these improvements and decorations should also be regarded as the protection scope of the present invention.
Claims (7)
1. A flow cytometer based pressure control circuit for a fluidic module, comprising:
The pressure detection circuit comprises a power supply conversion circuit, a pressure acquisition circuit, a pressure detection circuit, a pressure adjustment circuit and a reference signal selection circuit;
the power supply conversion circuit is respectively in communication connection with the pressure acquisition circuit, the pressure detection circuit, the pressure adjustment circuit and the reference signal selection circuit;
the pressure acquisition circuit and the pressure adjusting circuit are respectively in communication connection with the input end of the pressure detection circuit; the output end of the pressure detection circuit is in communication connection with the pressure adjusting circuit;
when the pressure control circuit is powered by the power conversion circuit, the flowing pressure signal of the sheath fluid collected by the pressure collection circuit is input to the pressure detection circuit, compared with the reference pressure signal provided by the reference signal selection circuit and outputs the generated pressure difference, the pressure difference output by the pressure detection circuit is input to the pressure adjustment circuit, and the flow of the sheath fluid flowing through the pressure control valve is adjusted by the pressure adjustment circuit according to the pressure difference, so that the flowing pressure of the sheath fluid is controlled.
2. The pressure control circuit of claim 1, wherein the pressure acquisition circuit comprises: pressure sensors, R1, R2, C5, C6;
The signal detection ends of the pressure sensors are respectively connected with one ends of R1 and R2 in a communication way; the other end of R2 is grounded; the other end of the R1 is connected with the pressure detection circuit in a communication way;
The signal detection end of the pressure sensor is also in communication connection with one ends of C5 and C6, and the other ends of C5 and C6 are grounded.
3. The pressure control circuit of claim 2, wherein the pressure detection circuit comprises: a comparison amplifier;
The reverse input end of the comparison amplifier is in communication connection with the other end of the R1 in the pressure acquisition circuit;
The positive input end of the comparison amplifier is in communication connection with the reference signal selection circuit;
the output end of the comparison amplifier is connected with the pressure adjusting circuit in a communication mode.
4. The pressure control circuit of claim 3, wherein the reference signal selection circuit comprises: an analog voltage gating chip, R3, R4, R5, VR1, VR2, VR 3;
One end of R3 is connected with a power supply, the other end of R3 is connected with one end of VR1, and the other end of VR1 is grounded; the common connection end of the R3 and the VR1 is in communication connection with the first signal gating end of the analog voltage gating chip;
the common connection end of the R4 and the VR2 is in communication connection with the second signal gating end of the analog voltage gating chip;
the common connection end of the R5 and the VR3 is in communication connection with the third signal gating end of the analog voltage gating chip;
And the communication end of the analog voltage gating chip is in communication connection with the positive input end of a comparison amplifier in the pressure detection circuit.
5. the pressure control circuit of claim 4, further comprising: a central processing unit;
and the central processing unit is respectively in communication connection with each signal switching control end of the analog voltage gating chip in the reference signal selection circuit.
6. The pressure control circuit of claim 3, wherein the pressure adjustment circuit comprises: a switching tube and a pressure control valve;
the collector end of the switching tube is in communication connection with the output end of a comparison amplifier in the pressure detection circuit;
The collector terminal of the switching tube is in communication connection with the power conversion circuit;
The emitter end of the switching tube is in communication connection with the power supply end of the pressure control valve;
The ground end of the pressure control valve is grounded.
7. The pressure control circuit of any of claims 1-6, wherein the power conversion circuit comprises: the first voltage stabilizing chip and the second voltage stabilizing chip are connected in series;
The power supply input end of the first voltage stabilizing chip is in communication connection with a power supply end, and the voltage output end of the first voltage stabilizing chip outputs a first power supply voltage;
The power supply input end of the second voltage stabilizing chip is in communication connection with the power supply end, and the voltage output end of the second voltage stabilizing chip outputs a second power supply voltage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920434818.2U CN209764680U (en) | 2019-04-02 | 2019-04-02 | Pressure control circuit of fluid module based on flow cytometer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920434818.2U CN209764680U (en) | 2019-04-02 | 2019-04-02 | Pressure control circuit of fluid module based on flow cytometer |
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| CN209764680U true CN209764680U (en) | 2019-12-10 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109916805A (en) * | 2019-04-02 | 2019-06-21 | 无锡厦泰生物科技有限公司 | A kind of pressure control circuit of the fluid modules based on flow cytometer |
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2019
- 2019-04-02 CN CN201920434818.2U patent/CN209764680U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109916805A (en) * | 2019-04-02 | 2019-06-21 | 无锡厦泰生物科技有限公司 | A kind of pressure control circuit of the fluid modules based on flow cytometer |
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