CN209753133U - Centrifugal equipment for cell culture - Google Patents

Centrifugal equipment for cell culture Download PDF

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Publication number
CN209753133U
CN209753133U CN201920599279.8U CN201920599279U CN209753133U CN 209753133 U CN209753133 U CN 209753133U CN 201920599279 U CN201920599279 U CN 201920599279U CN 209753133 U CN209753133 U CN 209753133U
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CN
China
Prior art keywords
centrifugal
bearing
shell
transmission shaft
gear
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201920599279.8U
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Chinese (zh)
Inventor
郭鸿
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanjing Yifeixue Biotechnology Co Ltd
Original Assignee
Nanjing Yifeixue Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Nanjing Yifeixue Biotechnology Co Ltd filed Critical Nanjing Yifeixue Biotechnology Co Ltd
Priority to CN201920599279.8U priority Critical patent/CN209753133U/en
Application granted granted Critical
Publication of CN209753133U publication Critical patent/CN209753133U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Centrifugal Separators (AREA)

Abstract

The utility model discloses a centrifugal equipment for cell culture, including casing, motor, transmission shaft, driven shaft, centrifuge bowl, loading board and apron, its characterized in that: the centrifugal pump is characterized in that a first bearing is arranged on the inner wall of the shell, the motor is arranged on the outer wall of the shell, the transmission shaft is arranged in the shell, one end of the transmission shaft is connected with the motor, the other end of the transmission shaft is arranged in the first bearing, the driven shaft vertically penetrates through a second bearing, the centrifugal cylinder is arranged in the shell, the bearing plate is movably arranged on the cushion block and provided with a bearing ring, and the cover plate is connected with the top end of the shell. The utility model discloses be provided with the anchorage bar through the spring in the carrier ring, the cell culture test tube that will need centrifugal treatment is vertical to be put into between anchorage bar and the anchorage bar, and the elasticity of spring promotes the anchorage bar and to test tube production pressure, makes the test tube firmly fix between anchorage bar and anchorage bar, improves the stability of test tube in centrifugal treatment process, and then improves centrifugal effect.

Description

Centrifugal equipment for cell culture
Technical Field
The utility model relates to a cell culture technical field specifically is a centrifugal equipment for cell culture.
Background
The cell separation technology is a branch of cell culture, centrifugation is a basic means for researching cell nucleus, mitochondria, Golgi body, lysosome and microbody and various macromolecules, and the cells need to be centrifugally rotated, but in the prior art, the cultured cells are generally taken out from an incubator and then transferred to a separate centrifugal device for centrifugation, so that the operation is complex, the stability of a cell culture test tube in the centrifugation process is poor, the possibility of cell pollution is increased, and the existing centrifugal device also has the problems of complex structure and high operation cost.
Disclosure of Invention
The utility model aims at overcoming the problem that the structure that centrifugal equipment for cell culture exists is complicated, inconvenient operation, running cost are high, cell culture test tube stability is not good in centrifugal process, providing a centrifugal equipment for cell culture that structural design is reasonable, work efficiency is high, convenient operation, cell culture test tube stability is good, running cost is low in centrifugal process.
the utility model provides a technical scheme that technical problem taken does:
The utility model provides a centrifugal equipment for cell culture, includes casing, motor, transmission shaft, driven shaft, centrifuge bowl, loading board and apron, its characterized in that: the inner wall of the shell is provided with a first bearing, a transverse fixing plate is arranged in the shell above the first bearing, a second bearing penetrating through the fixing plate up and down is arranged on the fixing plate, the motor is arranged on the outer wall of the shell, a power cord is arranged on the motor, the transmission shaft is arranged in the shell, one end of the transmission shaft is connected with the motor, the other end of the transmission shaft is arranged in the first bearing, a first gear is arranged on the transmission shaft, the driven shaft vertically penetrates through the second bearing, a second gear is arranged at the bottom end of the driven shaft, the centrifugal cylinder is arranged in the shell, the centrifugal cylinder is connected with the top end of the driven shaft, a cushion block is arranged on the inner wall of the centrifugal cylinder, the bearing plate is movably arranged on the cushion block, a bearing ring is arranged on the bearing plate, the centrifugal cylinder in the rotating process drives the cushion block to, the loading board drives the vertical test tube which is provided with cells and placed in the loading ring to rotate, centrifugal treatment is carried out on the cells, the centrifugal treatment efficiency and the centrifugal treatment quality of the cells are improved, the cover plate is connected with the top end of the shell, the cover plate is connected with the shell through threads, and in the centrifugal treatment process of the cells, the cover plate covers the shell, so that the centrifugal equipment is in a sealing state, and the safety in the centrifugal process of the cells is improved.
Preferably, the first gear and the second gear are respectively provided with an isosceles trapezoid cross section, the first gear is connected with the second gear, the motor drives the transmission shaft to rotate, the first gear on the transmission shaft drives the second gear on the driven shaft to rotate, so that the driven shaft and the centrifugal cylinder on the driven shaft are driven to rotate, the test tube filled with cells is subjected to centrifugal treatment, the rotating speed of the motor or the matching ratio of the first gear and the second gear is controlled according to the rotating speeds required by different cell centrifuges, and the mode of replacing the first gear on the transmission shaft or replacing the second gear on the driven shaft can be adopted by selecting a proper matching ratio.
Preferably, the loading board on be provided with the anchorage bar through the spring in the carrier ring, and set up the anchorage bar into the arc structure, the vertical bearing ring that inserts of test tube that will be equipped with the cell, the outer wall of test tube produces the extrusion to the anchorage bar, anchorage bar compression spring, the elasticity of spring also enables the anchorage bar and produces the extrusion to the test tube, make the test tube reach balanced state in the carrier ring, improve the test tube and at the stability of centrifugal processing in-process in the carrier ring, reinforcing cell centrifugal processing quality, set up the anchorage bar into the arc structure, make the anchorage bar present the top, the bottom is big, the thin hourglass shape structure in middle part, be convenient for insert between anchorage bar and the anchorage bar with the test tube that needs centrifugal processing, also be convenient for take out the test tube after the centrifugal processing from between anchorage bar and the anchorage bar.
Preferably, the apron on be provided with spacing post perpendicularly, the apron passes through the screw thread and is connected with the casing, make spacing post on the apron can play limiting displacement to the loading board, centrifuge tube in the rotation process drives the cushion rotatory, the cushion drives the loading board of placing on the cushion rotatory, the loading board drives vertical the test tube rotation of being equipped with the cell of putting in the bearing ring, carry out centrifugal treatment to the cell, can avoid the loading board to be bounced at rotation process through spacing post, strengthen the stability of cell centrifugal treatment in-process, and then strengthen cell centrifugal treatment quality.
The working principle is as follows: connect power cord on the motor with external power source, in the test tube vertical insertion that will be equipped with the cell bears the weight of the intra-annular, the outer wall of test tube produces the extrusion to the anchorage bar, anchorage bar compression spring, the elasticity of spring also enables the anchorage bar to produce the extrusion to the test tube, make the test tube reach balanced state in bearing the intra-annular, place the loading board on the cushion in the centrifuge tube, rotatory apron, be connected apron and casing, make spacing post on the apron play limiting displacement to the loading board, the starter motor, the motor drives the transmission shaft rotation, epaxial gear one drives two rotations of gear on the driven shaft, thereby it is rotatory to drive the centrifuge tube on driven shaft and the driven shaft, carry out centrifugal treatment to the test tube that is equipped with the cell, after the centrifugal treatment, take off the apron, take out the loading board, take off.
Has the advantages that: the utility model discloses be provided with the anchorage bar through the spring in the carrier ring, the cell culture test tube that will need centrifugal treatment is vertical to be put into between anchorage bar and the anchorage bar, and the elasticity of spring promotes the anchorage bar and to test tube production pressure, makes the test tube firmly fix between anchorage bar and anchorage bar, improves the stability of test tube in centrifugal treatment process, and then improves centrifugal effect.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic view of a part of the structure of the present invention, illustrating a connection structure of the first gear and the second gear.
Fig. 3 is a schematic view of a part of the structure of the present invention, illustrating the connection structure between the centrifugal cylinder and the cushion block.
Fig. 4 is a schematic partial structural diagram of the present invention, illustrating a connection structure of the bearing plate and the bearing ring.
fig. 5 is a schematic view of a part of the structure of the present invention, illustrating the connection structure of the cover plate and the limit post.
Fig. 6 is a schematic view of a part of the structure of the present invention, illustrating the connection structure of the load ring and the fastening rod.
Fig. 7 is a schematic diagram of another embodiment of the present invention.
Fig. 8 is a schematic view of a portion of the structure of fig. 7 of the present invention, illustrating a connection structure of the limiting post and the pressing wheel.
In the figure: 1. the centrifugal pump comprises a shell, a motor, a transmission shaft, a driven shaft, a centrifugal cylinder, a bearing plate, a cover plate, a bearing I, a bearing II, a power line, a gear I, a gear II, a bearing block, a bearing ring, a spring, a fastening rod, a limiting column, a fixing groove, a connecting shaft, a pressing wheel and a spring, wherein the motor is 2, the transmission shaft is 3, the driven shaft is 4, the centrifugal cylinder is 5, the bearing plate is 6, the cover plate is 7, the bearing I is 8, the fixing plate is 9, the.
Detailed Description
The present invention will be described in more detail with reference to the accompanying drawings.
The first embodiment is as follows:
As shown in the attached figures 1-6, a centrifugal device for cell culture comprises a shell 1, a motor 2, a transmission shaft 3, a driven shaft 4, a centrifugal cylinder 5, a bearing plate 6 and a cover plate 7, and is characterized in that: the inner wall of the shell 1 is provided with a bearing I8, a transverse fixing plate 9 is arranged in the shell 1 above the bearing I8, a bearing II 10 which vertically penetrates through the fixing plate 9 is arranged on the fixing plate 9, the motor 2 is arranged on the outer wall of the shell 1, a power cord 11 is arranged on the motor 2, the transmission shaft 3 is arranged in the shell 1, one end of the transmission shaft 3 is connected with the motor 2, the other end of the transmission shaft is arranged in the bearing I8, a gear I12 is arranged on the transmission shaft 3, the driven shaft 4 vertically penetrates through the bearing II 10, a gear II 13 is arranged at the bottom end of the driven shaft 4, the centrifugal cylinder 5 is arranged in the shell 1, the centrifugal cylinder 5 is connected with the top end of the driven shaft 4, a cushion block 14 is arranged on the inner wall of the centrifugal cylinder 5, the bearing plate 6 is movably arranged on the cushion block 14, and a bearing ring 15 is arranged, centrifugal cylinder 5 in the rotation process drives cushion 14 rotatory, and cushion 14 drives and places the loading board 6 rotation on cushion 14, and loading board 6 drives vertical test tube that is equipped with the cell of putting in bearing ring 15 rotatory, carries out centrifugal treatment to the cell, has improved cell centrifugal treatment efficiency and centrifugal treatment quality, apron 7 be connected with casing 1 top, pass through threaded connection with apron 7 and casing 1, in cell centrifugal treatment process, apron 7 covers casing 1, makes centrifugal equipment be in encapsulated situation, improves the security in the cell centrifugation process.
Preferably, the first gear 12 and the second gear 13 are both provided with isosceles trapezoid cross sections, the first gear 12 is connected with the second gear 13, the motor 2 drives the transmission shaft 3 to rotate, the first gear 12 on the transmission shaft 3 drives the second gear 13 on the driven shaft 4 to rotate, so that the driven shaft 4 and the centrifugal cylinder 5 on the driven shaft 4 are driven to rotate, the test tube with cells is subjected to centrifugal treatment, the rotating speed of the motor 2 or the matching ratio of the first gear 12 to the second gear 13 can be controlled according to the rotating speeds required by different cell centrifuges, and the mode of replacing the first gear 12 on the transmission shaft 3 or replacing the second gear 13 on the driven shaft 4 can be adopted by selecting a proper matching ratio.
preferably, bearing ring 15 on loading board 6 in be provided with anchorage bar 17 through spring 16, and set up anchorage bar 17 into the arc structure, the test tube that will be equipped with the cell is vertical to be inserted in bearing ring 15, the outer wall of test tube produces the extrusion to anchorage bar 17, anchorage bar 17 compression spring 16, 16 elasticity also enables anchorage bar 17 to produce the extrusion to the test tube, make the test tube reach balanced state in bearing ring 15, improve the test tube in bearing ring 15 and the stability in centrifugal treatment in-process, reinforcing cell centrifugal treatment quality, set up anchorage bar 17 into the arc structure, make anchorage bar 17 present the top, the bottom is big, the thin hourglass shape structure in middle part, be convenient for insert between anchorage bar 17 and the anchorage bar 17 the test tube that will need centrifugal treatment, also be convenient for take out the test tube after the centrifugal treatment from between anchorage bar 17 and the anchorage bar 17.
Preferably, the apron 7 on be provided with spacing post 18 perpendicularly, the apron 7 is connected with casing 1 through the screw, make spacing post 18 on the apron 7 can play limiting displacement to loading board 6, centrifuge tube 5 in the rotation process drives cushion 14 rotatory, cushion 14 drives loading board 6 of placing on cushion 14 rotatory, loading board 6 drives vertical test tube that is equipped with the cell of putting in bearing ring 15 rotatory, carry out centrifugal treatment to the cell, can avoid loading board 6 to be bounced at the rotation in-process through spacing post 18, strengthen the stability in the cell centrifugal treatment process, and then strengthen cell centrifugal treatment quality.
Example two:
As shown in the attached figures 7-8, a centrifugal device for cell culture comprises a shell 1, a motor 2, a transmission shaft 3, a driven shaft 4, a centrifugal cylinder 5, a bearing plate 6 and a cover plate 7, and is characterized in that: the inner wall of the shell 1 is provided with a bearing I8, a transverse fixing plate 9 is arranged in the shell 1 above the bearing I8, a bearing II 10 which vertically penetrates through the fixing plate 9 is arranged on the fixing plate 9, the motor 2 is arranged on the outer wall of the shell 1, a power cord 11 is arranged on the motor 2, the transmission shaft 3 is arranged in the shell 1, one end of the transmission shaft 3 is connected with the motor 2, the other end of the transmission shaft is arranged in the bearing I8, a gear I12 is arranged on the transmission shaft 3, the driven shaft 4 vertically penetrates through the bearing II 10, a gear II 13 is arranged at the bottom end of the driven shaft 4, the centrifugal cylinder 5 is arranged in the shell 1, the centrifugal cylinder 5 is connected with the top end of the driven shaft 4, a cushion block 14 is arranged on the inner wall of the centrifugal cylinder 5, the bearing plate 6 is movably arranged on the cushion block 14, and a bearing ring 15 is arranged, centrifugal cylinder 5 in the rotation process drives cushion 14 rotatory, and cushion 14 drives and places the loading board 6 rotation on cushion 14, and loading board 6 drives vertical test tube that is equipped with the cell of putting in bearing ring 15 rotatory, carries out centrifugal treatment to the cell, has improved cell centrifugal treatment efficiency and centrifugal treatment quality, apron 7 be connected with casing 1 top, pass through threaded connection with apron 7 and casing 1, in cell centrifugal treatment process, apron 7 covers casing 1, makes centrifugal equipment be in encapsulated situation, improves the security in the cell centrifugation process.
Preferably, the first gear 12 and the second gear 13 are both provided with isosceles trapezoid cross sections, the first gear 12 is connected with the second gear 13, the motor 2 drives the transmission shaft 3 to rotate, the first gear 12 on the transmission shaft 3 drives the second gear 13 on the driven shaft 4 to rotate, so that the driven shaft 4 and the centrifugal cylinder 5 on the driven shaft 4 are driven to rotate, the test tube with cells is subjected to centrifugal treatment, the rotating speed of the motor 2 or the matching ratio of the first gear 12 to the second gear 13 can be controlled according to the rotating speeds required by different cell centrifuges, and the mode of replacing the first gear 12 on the transmission shaft 3 or replacing the second gear 13 on the driven shaft 4 can be adopted by selecting a proper matching ratio.
Preferably, bearing ring 15 on loading board 6 in be provided with anchorage bar 17 through spring 16, and set up anchorage bar 17 into the arc structure, the test tube that will be equipped with the cell is vertical to be inserted in bearing ring 15, the outer wall of test tube produces the extrusion to anchorage bar 17, anchorage bar 17 compression spring 16, 16 elasticity also enables anchorage bar 17 to produce the extrusion to the test tube, make the test tube reach balanced state in bearing ring 15, improve the test tube in bearing ring 15 and the stability in centrifugal treatment in-process, reinforcing cell centrifugal treatment quality, set up anchorage bar 17 into the arc structure, make anchorage bar 17 present the top, the bottom is big, the thin hourglass shape structure in middle part, be convenient for insert between anchorage bar 17 and the anchorage bar 17 the test tube that will need centrifugal treatment, also be convenient for take out the test tube after the centrifugal treatment from between anchorage bar 17 and the anchorage bar 17.
Preferably, the apron 7 on be provided with spacing post 18 perpendicularly, the apron 7 is connected with casing 1 through the screw, make spacing post 18 on the apron 7 can play limiting displacement to loading board 6, centrifuge tube 5 in the rotation process drives cushion 14 rotatory, cushion 14 drives loading board 6 of placing on cushion 14 rotatory, loading board 6 drives vertical test tube that is equipped with the cell of putting in bearing ring 15 rotatory, carry out centrifugal treatment to the cell, can avoid loading board 6 to be bounced at the rotation in-process through spacing post 18, strengthen the stability in the cell centrifugal treatment process, and then strengthen cell centrifugal treatment quality.
Preferably, the bottom end of the limiting column 18 is provided with a fixing groove 19, a pressing wheel 21 is arranged in the fixing groove 19, a connecting shaft 20 is arranged on the pressing wheel 21, the connecting shaft 20 penetrates through the limiting columns 18 on two sides of the fixing groove 19, in the cell centrifugal treatment process, the pressing wheel 21 at the bottom end of the limiting column 18 presses the bearing plate 6, the bearing plate 6 in the rotating process drives the pressing wheel 21 to rotate, the limiting effect can be achieved on the bearing plate 6 in the rotating process, the resistance of the bearing plate 6 in the rotating process can also be reduced, and the cell centrifugal treatment efficiency and the centrifugal treatment quality are improved.
The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
In the description of the present invention, it is to be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings for convenience in describing the present invention and for simplicity in description, and are not intended to indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.
The utility model discloses the part that does not relate to all is the same with prior art or can adopt prior art to realize.

Claims (4)

1. The utility model provides a centrifugal equipment for cell culture, includes casing, motor, transmission shaft, driven shaft, centrifuge bowl, loading board and apron, its characterized in that: the centrifugal centrifuge comprises a shell, a bearing I, a horizontal fixing plate, a bearing II, a motor, a power line, a transmission shaft, a centrifugal cylinder and a bearing ring, wherein the bearing I is arranged on the inner wall of the shell, the horizontal fixing plate is arranged in the shell above the bearing I, the bearing II which penetrates through the fixing plate from top to bottom is arranged on the fixing plate, the motor is arranged on the outer wall of the shell, the power line is arranged on the motor, the transmission shaft is arranged in the shell, one end of the transmission shaft is connected with the motor, the other end of the transmission shaft is arranged in the bearing I, the gear I is arranged on the transmission shaft, the driven shaft vertically penetrates through the bearing II, the gear II is arranged at the bottom end of the driven shaft, the centrifugal cylinder is arranged in the shell, the centrifugal cylinder is connected with the top.
2. The centrifugal apparatus for cell culture according to claim 1, wherein: the cross sections of the first gear and the second gear are both of isosceles trapezoid structures, and the first gear is connected with the second gear.
3. The centrifugal apparatus for cell culture according to claim 1, wherein: the bearing ring on the bearing plate is internally provided with a fastening rod through a spring, and the fastening rod is of an arc-shaped structure.
4. The centrifugal apparatus for cell culture according to claim 1, wherein: and the cover plate is vertically provided with a limiting column.
CN201920599279.8U 2019-04-28 2019-04-28 Centrifugal equipment for cell culture Expired - Fee Related CN209753133U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201920599279.8U CN209753133U (en) 2019-04-28 2019-04-28 Centrifugal equipment for cell culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201920599279.8U CN209753133U (en) 2019-04-28 2019-04-28 Centrifugal equipment for cell culture

Publications (1)

Publication Number Publication Date
CN209753133U true CN209753133U (en) 2019-12-10

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201920599279.8U Expired - Fee Related CN209753133U (en) 2019-04-28 2019-04-28 Centrifugal equipment for cell culture

Country Status (1)

Country Link
CN (1) CN209753133U (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111036420A (en) * 2020-01-23 2020-04-21 中科宝承生物医学科技有限公司 High-efficiency centrifugal equipment for bispecific antibody and operation method thereof
CN112264200A (en) * 2020-09-30 2021-01-26 北京运峰晟合科贸有限公司 Marine invertebrate cell culture medium

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111036420A (en) * 2020-01-23 2020-04-21 中科宝承生物医学科技有限公司 High-efficiency centrifugal equipment for bispecific antibody and operation method thereof
CN112264200A (en) * 2020-09-30 2021-01-26 北京运峰晟合科贸有限公司 Marine invertebrate cell culture medium

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GR01 Patent grant
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CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20191210

Termination date: 20210428