CN209752915U

CN209752915U - Multichannel liquid drop generating device based on macroporous perfusion microspheres

Info

Publication number: CN209752915U
Application number: CN201821128405.3U
Authority: CN
Inventors: 王晓飞; 张博
Original assignee: Nanjing Yichun Technology Co Ltd
Current assignee: Nanjing Yichun Technology Co Ltd
Priority date: 2018-07-17
Filing date: 2018-07-17
Publication date: 2019-12-10
Anticipated expiration: 2028-07-17

Abstract

A multi-channel droplet generation device based on macroporous perfusion microspheres relates to the field of microfluidic droplet generation. The liquid drop generating device is provided with a capillary tube, a macroporous perfusion microsphere, a liquid conveying device and a liquid drop collecting device; the macroporous perfusion microspheres are fixed at the end ports of the capillary tubes and are provided with a plurality of through-holes; the capillary is a disperse phase channel; the liquid conveying device is used for conveying dispersed phases; the droplet collection device is for holding the continuous phase and for collecting droplets. The defect of the existing liquid drop preparation is overcome, the density of a liquid drop preparation channel is obviously improved by utilizing rich and uniform pores in the macroporous perfusion microspheres, the liquid drop generation flux is greatly improved, the large-scale preparation of liquid drops can be realized in a short time, the high uniformity of the size of the liquid drops can be controlled, the batch parallel liquid drop preparation is easy to realize, and the method has huge application potential.

Description

Multichannel liquid drop generating device based on macroporous perfusion microspheres

Technical Field

The utility model relates to a micro-fluidic droplet generates the field, especially relates to multichannel droplet generation device based on macropore perfusion microballon.

Background

The microfluidic droplet preparation technology is a brand-new tiny droplet preparation and operation technology developed in recent years, and divides fluid into droplets with certain volumes by utilizing two immiscible liquid phases and utilizing shearing force, surface tension and the like. Due to the advantages of micro-droplets in mass transfer, heat transfer, mass exchange, quantitative control, etc., micro-fluidic droplet technology has attracted extensive attention and research in recent years.

At present, the preparation of the liquid drops mainly forms the liquid drops through the crossing and relative movement between a continuous phase and a dispersed phase, for example, the dispersed phase and the continuous phase are processed by a T-shaped channel method, a flow focusing method and a coaxial flow method by a pressure pump, the dispersed phase liquid generates the liquid drops under the action of the continuous phase, and the three methods are widely applied to a chip method and a capillary channel method. In practical application, however, power sources (pressure pumps, injection pumps and the like) of the dispersed phase and the continuous phase are easy to be unstable in a long-time operation process, so that the uniformity and consistency of the generated size of the liquid drops are influenced; another disadvantage is that high density multi-channel parallelism cannot be achieved, the droplet volumes are all on the nanoliter scale, or smaller, and how to achieve large scale production is also a significant challenge.

Disclosure of Invention

The utility model aims at providing a droplet generation device is simple, the secondary reproducibility is good, easily realize the preparation of parallel liquid drop in batches, makes liquid drop preparation flux and precision obtain showing a multichannel droplet generation device based on macropore perfusion microballon that improves.

The utility model is provided with a capillary tube, a macroporous perfusion microsphere, a liquid conveying device and a liquid drop collecting device; the macroporous perfusion microspheres are fixed at the end ports of the capillary tubes and are provided with a plurality of through-holes; the capillary is a disperse phase channel; the liquid conveying device is used for conveying dispersed phases; the droplet collection device is for holding the continuous phase and for collecting droplets.

And the dispersed phase is conveyed into the capillary tube by a liquid conveying device at a certain flow rate, is cut into liquid drops with a certain size after passing through a pore channel in the macroporous perfusion microsphere, and is collected in a container for containing the continuous phase.

the liquid delivery device can adopt a syringe pump, a syringe barrel, a peristaltic pump, a high-pressure pump and the like which can deliver liquid at accurate flow rate.

The utility model discloses in have abundant micron order pore based on macropore perfusion microballon inside for realize the purpose that multichannel liquid drop generated.

The utility model discloses the material of well used capillary can adopt organic polymer material or inorganic material, organic polymer material can adopt polyether ether ketone (PEEK) etc., inorganic material can adopt quartzy, metal etc.. The inner diameter of the capillary tube can be 10-500 μm.

The material of the medium-large hole perfusion microsphere can be silicon dioxide inorganic material, organic polymer material, organic-inorganic hybrid material, and the large hole perfusion microsphere contains rich large holes with about 1 mu m inner diameter.

The method for fixing the middle-large hole perfusion microspheres at the capillary port of the utility model can be based on the physical methods such as the keystone Effect (Key Stone Effect) and the thermal deformation mechanical extrusion, and can also be based on the chemical reaction method for bonding at the nozzle.

The utility model discloses after the well disperse phase prepares out the liquid drop through macropore perfusion microballon, based on the difference of continuous phase and disperse phase density, the liquid drop can come up to the continuous phase liquid level, also can sink to continuous phase bottom below, or homodisperse inside the continuous phase.

The utility model discloses in the single drop preparation process, can parallelly use many capillaries that are equipped with macropore perfusion microballon, realize the preparation of the parallel multichannel drop of multichannel.

The preparation method of the utility model is as follows:

Based on the kerbstone effect, fixing a perfusion silicon ball at the end of a capillary, placing one end of the capillary with the macroporous perfusion microspheres into a container filled with a fluorocarbon-40 continuous phase, connecting the other end of the capillary with the macroporous perfusion microspheres with a PEEK (polyetheretherketone) hose and an injector, and pressing the continuous phase into the capillary by taking water/methanol (1/1, v/v) as a dispersion phase, thereby quickly generating a large amount of liquid drops.

The capillary tube can be a quartz capillary tube, the inner diameter of the capillary tube is 100 mu m, and the length of the capillary tube is 10 cm; the outer diameter of the perfusion silicon ball can be 100 microns, and 1mL of continuous phase can be manually pressed into the capillary by pressing the continuous phase into the capillary.

The utility model discloses well liquid drop generates the device simply, and liquid drop preparation flux is high, and liquid drop size reproducibility is good.

The utility model overcomes the shortcoming of current liquid drop preparation utilizes the inside abundant even hole of macropore perfusion microballon, is showing the density that has improved the liquid drop preparation passageway, has improved the liquid drop and has generated flux by a wide margin, can realize a large amount of preparations of liquid drop in the short time, also can control the high homogeneity of liquid drop size simultaneously, and easily realize the preparation of parallel liquid drop in batches, has huge application potentiality.

Drawings

Fig. 1 shows the set-up of a capillary droplet generator.

FIG. 2 is a schematic diagram of a single capillary multi-channel droplet preparation apparatus in example 1.

Fig. 3 is a schematic diagram of a parallel 8 multi-channel capillary drop generating device in example 2.

FIG. 4 is an optical microscope photograph of highly uniform sized droplets obtained in example 3.

Detailed Description

The invention will be further described with reference to the drawings and several alternative embodiments. It is to be noted that: the present invention is not limited to the following embodiments. The examples do not show the specific techniques and conditions, and the reagents and apparatus are not shown in the manufacturers, and the reagents and apparatus are all conventional products commercially available, according to the techniques and conditions described in the literature in the field or according to the specifications of the products.

Example 1: construction of multi-channel liquid drop generating device with inner diameter of 100 mu m

Referring to fig. 1 and 2, a 10cm capillary tube 3 with an inner diameter of 100 μm is cut, a dispersion phase 10 is pressed into the parallel capillary tube 3 by an injection pump 5, a macroporous perfusion silica sphere 7 with an outer diameter of 100 μm is fixed at the port of the capillary tube 3 based on the keystone effect, one end of the capillary tube 3 with macroporous perfusion microspheres is placed into a container 8 containing a fluorocarbon-40 (FC-40)4 continuous phase, the other end of the capillary tube 3 is connected with an injector 1 by a PEEK hose 2, water/methanol (1/1, v/v) is used as the dispersion phase 10, and 1mL of the continuous phase 9 is manually pressed into the capillary tube 3, so that a large amount of droplets 11 can be rapidly generated.

Example 2: construction of parallel 8-multi-channel capillary liquid drop generating device

8 quartz capillaries 3 having a length of 10cm and an inner diameter of 100 μm were cut out. Using the method of example 1, a perfused silica sphere with an outer diameter of 100 μm was fixed at the port of each capillary based on the keystone effect, one end of the capillary having the macroporous perfused microspheres was placed in a container containing FC-404 continuous phase, the other end was connected to an injector using PEEK tubing 2, and the dispersed phase was split into 8 channels using four tee 6 devices (see fig. 3). Water/methanol (1/1, v/v) is used as a dispersion phase, and the dispersion phase is pressed into 8 parallel capillary tubes 3 at the flow rate of 500 mu L/min by using a syringe pump 5, so that the construction of a parallel multi-channel capillary tube droplet generation device is realized.

Example 3: generation of highly uniform sized droplets

With the droplet generation apparatus set up in example 1, FC-40 was used as the continuous phase, water/methanol (1/1, v/v) was used as the dispersed phase, the dispersed phase was injected into the continuous phase at a flow rate of 50 μ L/min with a precision syringe pump to prepare droplets, and the sizes of the droplets were observed and measured under a microscope, and by measuring the diameters of 50 droplets, the CV value of the resulting droplets was calculated to be 3%, indicating that the resulting droplets were highly uniform in size (fig. 4).

The utility model discloses constitute by capillary, macropore perfusion microballon, liquid conveyor and liquid drop collection device: the macroporous perfusion microspheres are fixed at the end of the capillary, then the capillary is placed under the liquid level of the continuous phase, and the dispersed phase is injected into the continuous phase through the capillary at a certain flow rate. When the dispersion phase passes through the macroporous perfusion microspheres, a multichannel microfluidic flow is formed through rich and uniform pores in the macroporous perfusion microspheres, and the multichannel microfluidic flow is cut into liquid drops at the pore outlets. The preparation device is simple, the method is reliable, and the obtained liquid drops have good size uniformity; a large number of pore channels with the diameter of 1 mu m penetrate through the microspheres to form a plurality of liquid drop generating micro-channels, so that the liquid drop generating flux is greatly improved; the device is used for preparing the liquid drops, the accurate control of the size and the generated flux of the liquid drops is easier to realize, and the defects of small number of channels, easy blockage, low generated flux of the liquid drops and the like of the conventional microfluidic chip are overcome.

Claims

1. A multi-channel liquid drop generating device based on macroporous perfusion microspheres is characterized by being provided with a capillary tube, macroporous perfusion microspheres, a liquid conveying device and a liquid drop collecting device; the macroporous perfusion microspheres are fixed at the end ports of the capillary tubes and are provided with a plurality of through-holes; the capillary is a disperse phase channel; the liquid conveying device is used for conveying dispersed phases; the liquid drop collecting device is used for containing the continuous phase and collecting liquid drops; the liquid conveying device adopts an injection pump, an injector needle cylinder, a peristaltic pump and a high-pressure pump; the inner diameter of the capillary tube is 10-500 mu m.

2. The macroporous perfusion microsphere-based multi-channel droplet generation device of claim 1, wherein the macroporous perfusion microspheres comprise macropores with an inner diameter of 1 μm.