CN209485976U - A kind of dedicated infrared transflector spectral measurement attachment of glass slide sample preparation - Google Patents
A kind of dedicated infrared transflector spectral measurement attachment of glass slide sample preparation Download PDFInfo
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- CN209485976U CN209485976U CN201822279777.2U CN201822279777U CN209485976U CN 209485976 U CN209485976 U CN 209485976U CN 201822279777 U CN201822279777 U CN 201822279777U CN 209485976 U CN209485976 U CN 209485976U
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- glass slide
- transflector
- sample preparation
- infrared
- attachment
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Abstract
The utility model discloses a kind of dedicated infrared transflector spectral measurement attachmentes of glass slide sample preparation, the attachment is hollow hood-like structure, for covering on glass slide with completely obscured glass slide but not touching the sample on glass slide, the hood-like structure includes roof and the side wall that extends downwardly from roof, the inner surface of the roof is the plane parallel with glass slide and is covered with reflectorized material and is used to form reflection layer, and the inner surface of the side wall is black and opaque.The utility model attachment is suitable for the infrared spectrometric analyzer configured with integrating sphere accessory, it can be under the premise of not influencing the original devices such as infrared spectrometric analyzer optical path, realize the infrared transflector spectral measurement of glass slide sample preparation, overcome operating difficulties existing when existing infrared spectrometric analyzer measurement attachment does not support the spectrometric insufficient and prior art measurement glass slide sample preparation of the transflector of glass slide sample preparation, it is cumbersome the defects of, have the advantages that assembly is simple, detects accurately and rapidly.
Description
Technical field
The utility model relates to a kind of device for being convenient for infrared transflector spectral measurement, in particular to a kind of glass slides
The dedicated infrared transflector spectral measurement attachment of sample preparation.
Background technique
Biological tissue or cell are carried using glass slide, using the methods of slice, smear, tabletting, load, is made for micro-
The sample of microscopic observation or imaging analysis, is the common sample making technology of biological detection, is widely used in biology, medicine (such as disease
Of science, pestology), agronomy (such as Plant Pathology) field.But if the above morphological analysis being capable of binding molecule water
The characteristic of flat quantitative information, biological sample will be characterized more effectively.
Infrared spectroscopy because have many advantages, such as it is quick, lossless, and equipped with being suitable for transmission measurement mode
Sample cell, the integrating sphere accessory for being suitable for diffusing reflection measurement pattern and sample rotates cup are suitable for decaying total reflection measurement pattern
ATR attachment, be suitable near line detection optical fiber attachment, even have dedicated tablet transflector measurement attachment, so infrared light
Spectrum analysis method is widely used in petroleum (bibliography: petroleum component of Qian Ping, Sun Guoqin, the Zhang Cunzhou based on near-infrared spectrum technique
Quantitative analysis new method [J] spectroscopy and spectrum analysis .2008,28 (12): 2851-2854.), food (bibliography: Sun Zong
Protect, the fast qualitative of Fourier transform infrared spectroscopy the combination chemometrics method dialogue wine base wine such as Xin Xin, Zou little Bo and calmly
Amount analysis [J] spectroscopy and spectrum analysis .2017,37 (9): 2756-2762.), (bibliography: Yin Fei, Li Ke are close for drug
Application [J] Pharmaceutical Analysis magazine .2010 of the infrared spectroscopy drug quick identification technology in base's drug inspection, 30 (10):
The fields such as 1996-2000.).
Although existing research person carries out auxiliary diagnosis (reference to histopathologic slide using infrared diffusing reflection spectrum analytic approach
Document: the near-infrared spectrum technique combination Principal Component Analysis such as Xu Ke, Xiang Yuhong, Dai Yinmei is used for the diagnosis of carcinoma of endometrium
[J] Chemical Journal of Chinese Universities .2009,30 (8): 1543-1547.), but use when diffusing reflection mode usually because largely dividing
Analysis light passes through relatively thin tissue sample and glass slide and can not form reflection, and measurement is caused to fail.Infrared transflector spectrum be
It is generated on the basis of integrating sphere is irreflexive, existing infrared spectrometric analyzer measurement attachment does not support the transflector of glass slide sample preparation
There is the defects of operating difficulties, cumbersome again in spectroscopic assay, the infrared transflector spectrum using the measurement glass slide sample preparation of other methods.
Therefore, the utility model is intended to provide a kind of dedicated infrared transflector spectral measurement attachment of glass slide sample preparation, to cooperate infrared spectroscopy
Characteristic of the analyzer in molecular level quantization signifying biological sample.
Utility model content
In order to overcome existing infrared spectrometric analyzer measurement attachment not support the transflector of glass slide sample preparation spectrometric
Existing operating difficulties when the measurement glass slide sample preparation of the insufficient and prior art, it is cumbersome the defects of, the utility model provides
A kind of dedicated infrared transflector spectral measurement attachment of glass slide sample preparation, simply, easily to realize the accurate, fast of glass slide sample preparation
Speed and nondestructive analysis.
The dedicated infrared transflector spectral measurement attachment of the glass slide sample preparation of the utility model is hollow hood-like structure, is used for
It covers on glass slide with completely obscured glass slide but does not touch the sample on glass slide, the hood-like structure includes roof and Zi Ding
The side wall that wall extends downwardly, the inner surface of the roof are the plane parallel with glass slide and are covered with reflectorized material for shape
At reflection layer, the inner surface of the side wall is black and opaque.
Preferably, the reflectorized material is goldleaf.
Preferably, the hood-like structure is the open cuboid cover in bottom.
Glass slide sample preparation described in the utility model is (including to be cut using glass slide as carrier using existing microsection technology
Piece method, smear method, pressed disc method, load method etc.) sample observed under an optical microscope will be suitable for made of biomaterial.
The inner surface of the utility model attachment side wall is set as black and opaque, helps avoid stray light and surveys to spectrum
Fixed interference.
The dedicated infrared transflector spectral measurement attachment of the glass slide sample preparation of the utility model, for cooperating infrared spectrum analysis
Instrument carries out the infrared transflector spectral measurement of glass slide sample preparation, is suitable for current common producer, common model configured with integrating sphere
The infrared spectrometric analyzer of attachment can directly be covered on and be set under the premise of not influencing the original devices such as infrared spectrometric analyzer optical path
In integrating sphere detection window on glass slide above with completely obscured glass slide after, carry out the measurement of infrared transflector spectrum.Measurement
When, it is anti-by the reflection layer of attachment roof inner surface after the analysis light that projects penetrates glass slide sample preparation in integrating sphere detection window
It penetrates, penetrates glass slide sample preparation again in a reverse direction, detected after returning to integrating sphere by detector.Due to analysis light back and forth totally 2
Secondary to penetrate sample to be tested, the concentration for being equivalent to target substance improves 1 times (for 2 times of original content), effectively improves detection
Sensitivity, to obtain the characteristic information of target substance in sample to be tested.
The utility model has the beneficial effects that: the utility model provides a kind of dedicated infrared transflector of glass slide sample preparation
Spectral measurement attachment is mainly used for measuring the variation of biological sample molecular level, characterizes the characteristic of biological sample, such as predict pathology
Whether the tissue in histotomy occurs lesion and lesion degree etc. to assist pathological diagnosis.The utility model overcomes existing
Infrared spectrometric analyzer measurement attachment does not support the spectrometric insufficient and prior art measurement of the transflector of glass slide sample preparation
Existing operating difficulties when glass slide sample preparation, it is cumbersome the defects of, can simply, easily realize glass slide sample preparation it is accurate,
Quickly and nondestructive analysis, be biology, medicine (such as pathology, pestology), agronomy (such as Plant Pathology) field sample
Product analysis provides good spectral measurement auxiliary tool.
Detailed description of the invention
Fig. 1 is the stereochemical structure of the dedicated infrared transflector spectral measurement attachment of glass slide sample preparation of the utility model embodiment
Schematic diagram.In figure: 1 is roof, and 2 be side wall, and 3 be reflection layer.
Fig. 2 is Antaris II FT-NIR spectrometer (Thermo Fisher used in the utility model embodiment
Scientific structure composition schematic diagram).
Specific embodiment
With reference to the accompanying drawing, by specific embodiment, the utility model is further described.
The dedicated infrared transflector spectral measurement attachment of the glass slide sample preparation of the utility model, for cooperating infrared spectrum analysis
The infrared transflector spectral measurement of instrument progress glass slide sample preparation.
Fig. 1 shows the solid of the dedicated infrared transflector spectral measurement attachment of glass slide sample preparation of the utility model embodiment
Structure.As shown, the attachment is hollow hood-like structure, the hood-like structure includes roof 1 and extends downwardly from roof 1
Side wall 2, the inner surface of the roof 1 are the plane parallel with glass slide and are covered with reflectorized material and are used to form reflection layer
3, the inner surface of the side wall 2 is black and opaque.The inside dimension of the hood-like structure should ensure that when it covers on glass slide
When upper can the reflection layer on completely obscured glass slide and roof inner surface will not touch the sample on glass slide.
Specifically, the cuboid cover that the hood-like structure used in the utility model embodiment is open for bottom, cuboid cover
The inner surface covering goldleaf of roof forms reflection layer.
Specifically, the inside dimension of the cuboid cover be 80 × 29 × 5mm, greater than conventional slide size 76 × 25 ×
1mm。
When measuring the infrared transflector spectrum of glass slide sample preparation using the utility model attachment, first glass slide sample preparation is placed in
On the integrating sphere detection window of infrared spectrometric analyzer (overall diameter that the width of glass slide should be greater than unthreaded hole on integrating sphere detection window),
The dedicated infrared transflector spectral measurement attachment of the glass slide sample preparation of the utility model is directly covered on above glass slide with complete again
Glass slide is covered, the measurement of infrared transflector spectrum is then carried out.
In order to prove the feasibility of the utility model attachment, Antaris II FT-NIR spectrometer (Thermo is used
Fisher Scientific) integrating sphere diffusing reflection platform and the present embodiment the dedicated infrared transflector spectrum of glass slide sample preparation
The near-infrared transflector spectrum of attachment measurement Colorectal Carcinoma slice and normal mucosa slice is measured, to establish differentiation large intestine
The near-infrared transflector spectra methods of cancerous tissue.
Fig. 2 shows infrared spectrometric analyzer used in the present embodiment, that is, Antaris II FT-NIR spectrometer knots
Structure is mainly made of light source, beam splitting system, integrating sphere accessory, detector, control and display module.Wherein, the light source energy
In 12000~3800cm of wave-length coverage-1Inside launch stable, uniform, high-intensitive, continuous wavelength near infrared light；The light splitting
Complex light can be divided into monochromatic light by system；The integrating sphere accessory is for collecting the light to be detected from the different directions of propagation；
The detector is used to the optical signal for carrying sample message being converted to electric signal；It is described control with display module for regulate and control and
Show the operation of above each section.
Unthreaded hole overall diameter on the integrating sphere detection window of Antaris II FT-NIR spectrometer is 12.68mm, colorectal cancer group
The glass slide of slice and normal mucosa slice is knitted having a size of 76 × 25 × 1mm.
Method particularly includes: first collect 11 normal mucosas slice and 11 Colorectal Carcinomas slices respectively, then with
Resolution ratio 8cm-1, scanning times 64 times, in 10000~3800cm-1The near-infrared transflector spectrum of acquisition slice, institute in range
It is not preprocessed to obtain spectrum, selecting modeling spectral region is 9877~3922cm-1, using Principal Component Analysis to selected modeling light
The Data Dimensionality Reduction of spectral limit chooses preceding 5 principal components as Modelling feature variable, from 11 according to the sequence of contribution rate from high to low
Taken respectively in piece normal mucosa slice and 11 Colorectal Carcinomas slice 8 histotomies totally 16 histotomies as school
Positive collection slice, remaining is verifying collection slice, uses discriminant analysis using the principal component scores of calibration set and verifying collection spectroscopic data
(DA) method establishes the prediction model with verifying identification human body large intestine (cancer) tissue.The results show that the calibration set of built DA model is just
Sentencing rate is 91.67%, and verifying collection right judging rate is 88.89%.
Above data proves, can be surveyed using the dedicated infrared transflector spectral measurement attachment of the glass slide sample preparation of the present embodiment
Obtain high quality (containing tissue signature's information) spectrum of tissue slice.As it can be seen that using the glass slide system of the utility model
The dedicated infrared transflector spectral measurement attachment of sample can simply, easily realize accurate, quick and lossless point of glass slide sample preparation
Analysis.
Finally, it is stated that above embodiments are merely intended for describing the technical solutions of the present application, but not for limiting the present application, although logical
The preferred embodiment crossed referring to the utility model has been described the utility model, but those skilled in the art should manage
Solution, can make various changes, without departing from defined by the appended claims to it in the form and details
The protection scope of the utility model.
Claims (3)
1. a kind of dedicated infrared transflector spectral measurement attachment of glass slide sample preparation, which is characterized in that the attachment is hollow cover
Shape structure, for covering on glass slide with completely obscured glass slide but not touching the sample on glass slide, the hood-like structure packet
The side wall for including roof and extending downwardly from roof, the inner surface of the roof are the plane parallel with glass slide and are covered with anti-
Luminescent material is used to form reflection layer, and the inner surface of the side wall is black and opaque.
2. the dedicated infrared transflector spectral measurement attachment of a kind of glass slide sample preparation according to claim 1, it is characterised in that:
The reflectorized material is goldleaf.
3. the dedicated infrared transflector spectral measurement attachment of a kind of glass slide sample preparation according to claim 1 or 2, feature exist
In: the hood-like structure is the open cuboid cover in bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822279777.2U CN209485976U (en) | 2018-12-27 | 2018-12-27 | A kind of dedicated infrared transflector spectral measurement attachment of glass slide sample preparation |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201822279777.2U CN209485976U (en) | 2018-12-27 | 2018-12-27 | A kind of dedicated infrared transflector spectral measurement attachment of glass slide sample preparation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109490239A (en) * | 2018-12-27 | 2019-03-19 | 重庆医科大学 | A kind of dedicated infrared transflector spectral measurement attachment of glass slide sample preparation |
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2018
- 2018-12-27 CN CN201822279777.2U patent/CN209485976U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109490239A (en) * | 2018-12-27 | 2019-03-19 | 重庆医科大学 | A kind of dedicated infrared transflector spectral measurement attachment of glass slide sample preparation |
| CN109490239B (en) * | 2018-12-27 | 2024-02-02 | 重庆医科大学 | Special infrared transmission and reflection spectrum measurement accessory for glass slide sample preparation |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191011 Termination date: 20201227 |