CN209468444U - Cryopreservation tube stirs thawing apparatus - Google Patents

Abstract

The utility model discloses a kind of cryopreservation tubes to stir thawing apparatus, including handle, decelerating motor and fixed sleeving are fixedly connected sequentially from top to bottom, and battery is equipped in intracavity of handle and is connected with the deceleration motor by conducting wire and open-close on-off；Shaft is equipped with by bearing or axle sleeve in fixed sleeving, geared motor output shaft is connect with rotating shaft transmission, the part that shaft is stretched out except fixed sleeving is equipped with attachment base, the attachment base side is equipped with pin shaft and passes through the upper limb of the hinged funnel of the pin shaft, the bottom of funnel is provided with bottom outlet and gasket, cryopreservation tube sealing shroud is interior loaded on bottom outlet and gasket and pipe cap is located in funnel, and funnel brim height is higher than pipe cap thickness.The utility model is quickly stirred in thawing solution by controlling cryopreservation tube, make cryopreservation tube with thawing solution is full and uniform contacts, raising defrosting efficiency and cell survival rate.It is easy to operate, remote-controlled operation is carried out using handle and fixed sleeving, freezing liquid frostbite experimenter's arm can be prevented.

Description

Cryopreservation tube stirs thawing apparatus

Technical field

The utility model belongs to cryopreservation tube deforst technique field, and in particular to it is a kind of by way of control stirring to freezing Pipe carries out quick-thawing device.

Background technique

Freeze-stored cell recovery thaw that time be it is very crucial, to shorten dissolution time as far as possible, so as not to be formed ice Brilliant (making cellular damage because Free water condenses into ice crystal in film) scratches cell, begins to operate if not being completely dissolved, to thin The injury of born of the same parents is quite big.It is handled on super-clean bench if thawing just to take without complete solution, also results in dead cell than just having recovered When more factories it is a lot of.Freeze-stored cell is thawed frequently with Unfreezing apparatus ware with warm water when thawing, as Publication No. CN201634669U is mentioned The simple thermal insulation and anti-contamination cell thawing cup of confession can carry out batch defrosting to multiple groups cryovial simultaneously, easy to operate.Actually It thaws for experimental purposes to cryopreservation tube, without thawing multiple groups simultaneously, in contrast the defrosting quality requirement of every group of cryopreservation tube is more It is high.On the other hand, the liquid level of the thawing solution in thawing cup is deeper, but actually cryopreservation tube is in course of defrosting, pipe cap Liquid pollution cannot be defrosted, so cryopreservation tube can only thaw in thawing solution surface operation, due in thawing solution heat transfer speed compared with Slowly, so there are defrosting low efficiencys and insufficient problem of thawing.

Summary of the invention

The utility model at present to cryopreservation tube course of defrosting there are problems that defrosting low efficiency and it is inconvenient even The problem of being easy frostbite arm provides a kind of easy to operate and efficiently high-quality defrosting device.

Realize that technical solution used by above-mentioned purpose is to stir thawing apparatus using a kind of cryopreservation tube, including handle, subtract Speed motor and fixed sleeving are fixedly connected sequentially from top to bottom, and battery is equipped in intracavity of handle and is opened by conducting wire and opening and closing Pass connects with the deceleration motor, and open-close on-off is located at handle front end；Shaft is equipped with by bearing or axle sleeve in fixed sleeving, is subtracted Speed motor output shaft is connect with rotating shaft transmission, and the part that shaft is stretched out except fixed sleeving is equipped with attachment base, the attachment base one Side is equipped with pin shaft and passes through the upper limb of the hinged funnel of the pin shaft, and the bottom of funnel is provided with bottom outlet and sealing Pad, cryopreservation tube sealing shroud is interior loaded on bottom outlet and gasket and pipe cap is located in funnel, and it is thick that funnel brim height is higher than pipe cap Degree.

The attachment base side is equipped with pin shaft and passes through the upper limb of the hinged the taper bucket of the pin shaft, in the bottom of the taper bucket It is provided with bottom outlet and gasket,

Sealing cover is connected in the upper port side of funnel or the taper bucket.

The utility model has the beneficial effects that the utility model provides a kind of easy device that operates and can efficiently thaw, Cryopreservation tube need to only be moved in corresponding the taper bucket, quickly be stirred in thawing solution by switch control cryopreservation tube, make cryopreservation tube With thawing solution sufficiently and uniformly contact, can be improved defrosting efficiency and cell survival rate.The utility model is simple to operate, benefit Remote-controlled operation is carried out with handle and fixed sleeving, can prevent freezing liquid from may cause frostbite problem to experimenter's arm, Hand operated mixing can also be cooperated, further increase defrosting efficiency.

Detailed description of the invention

Fig. 1 is the use state diagram of the utility model.

Fig. 2 is the positive structure schematic of the utility model.

Fig. 3 is the utility model rotary state schematic diagram.

Figure label: 1 is thawing cup, and 2 be thawing solution, and 3 be cryopreservation tube, and 4 be pipe cap, and 5 be handle, and 6 be open-close on-off, 7 It is fixed sleeving for decelerating motor, 8,9 be shaft, and 10 be attachment base, and 11 be pin shaft, and 12 be the taper bucket, and 13 be bottom outlet and sealing Pad.

Specific embodiment

Embodiment 1: cryopreservation tube 3 stirs thawing apparatus as shown in Figure 1, for installing cryopreservation tube 3 and being inserted into thawing cup 1 and solution Freeze the contact of 2 upper layer of liquid, is usually not easy to be inserted into too deep to prevent the pipe cap 4 of thawing solution immersion cryopreservation tube 3 from polluting.Pass through opening and closing Switch 6 controls decelerating motor 7 and rotates, and then cryopreservation tube 3 is driven to rotate in thawing solution, and cryopreservation tube 3 is made to repeat to connect with thawing solution Quick uniform defrosting purpose is realized in touching.

As shown in Figure 1, which includes handle 5, decelerating motor 7 and fixed sleeving 8, and three is fixedly connected sequentially.Wherein, Handle 5 is tubulose, and containing inner cavity, Matching installation has battery in 5 inner cavity of handle, and open-close on-off 6 is located at 5 front end of handle, passes through Battery, open-close on-off 6 and decelerating motor 7 are cascaded by conducting wire.Open-close on-off 6 is preferably push button, by pressing and releasing Open-close on-off 6 is put, motor rotation can be controlled and is stopped.Decelerating motor 7 is common DC speed-reducing 7, and as motor exports One miniature gear box of axis connection realizes speed changing function, controls motor speed and gear stages realize 30-120 revs/min of output end The velocity interval of clock.

Figure it is seen that being equipped with shaft 9,7 output shaft of decelerating motor by bearing or axle sleeve in fixed sleeving 8 It is sequentially connected with shaft 9.The lower end of shaft 9 is stretched out except fixed sleeving 8, and the part of stretching is equipped with attachment base 10.

The present embodiment assembles single cryopreservation tube 3 using a taper bucket 12, and the bottom of the taper bucket 12 is provided with bottom Hole and gasket 13,3 sealing shroud of cryopreservation tube is interior loaded on bottom outlet and gasket 13 and pipe cap 4 is located in the taper bucket 12.In addition, connection The side of seat 10 is equipped with pin shaft 11, and passes through the upper limb of 11 hinged the taper buckets 12 of pin shaft.To the taper bucket 12 and its The cryopreservation tube 3 of inside is in hang in its natural state, as shown in figure 3, no matter how shaft 9 rotates, the taper bucket 12 is taken Band cryopreservation tube 3 prevents frozen stock solution from arranging near vertical state to prevent cryopreservation tube 3 from problem of significantly tumbling occur always It lets out.Since the brim height of the taper bucket 12 is higher than 4 thickness of pipe cap, freeze so can prevent thawing solution from entering pollution in the taper bucket 12 The pipe cap 4 of pipe is deposited, sealing cover can also be connected in the upper port side of the taper bucket 12.When motor driven stirs, it can also cooperate Hand operated mixing further increases defrosting efficiency.

Claims (3)

1. a kind of cryopreservation tube stirs thawing apparatus, it is characterised in that: including handle, decelerating motor and fixed sleeving from top to bottom according to It is secondary to be fixedly connected, battery is installed in intracavity of handle and is connected with the deceleration motor by conducting wire and open-close on-off, open-close on-off Positioned at handle front end；Shaft is equipped with by bearing or axle sleeve in fixed sleeving, geared motor output shaft and rotating shaft transmission connect It connects, the part that shaft is stretched out except fixed sleeving is equipped with attachment base, which is equipped with pin shaft and by the pin shaft The upper limb of a hinged funnel is provided with bottom outlet and gasket in the bottom of funnel, cryopreservation tube sealing shroud loaded on bottom outlet and In gasket and pipe cap is located in funnel, and funnel brim height is higher than pipe cap thickness.

2. cryopreservation tube according to claim 1 stirs thawing apparatus, it is characterised in that: the attachment base side is equipped with pin shaft And pass through the upper limb of the hinged the taper bucket of the pin shaft, the bottom of the taper bucket is provided with bottom outlet and gasket.

3. cryopreservation tube according to claim 1 or 2 stirs thawing apparatus, it is characterised in that: in funnel or the taper bucket Upper port side is connected with sealing cover.