CN209406785U - A kind of Wet-dry Meso-micro soil fauna separator - Google Patents

A kind of Wet-dry Meso-micro soil fauna separator Download PDF

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Publication number
CN209406785U
CN209406785U CN201920003067.9U CN201920003067U CN209406785U CN 209406785 U CN209406785 U CN 209406785U CN 201920003067 U CN201920003067 U CN 201920003067U CN 209406785 U CN209406785 U CN 209406785U
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separatory funnel
separator
cabinet
sample
sample holder
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CN201920003067.9U
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Inventor
刘继亮
潘成臣
杨淇越
赵文智
罗亚勇
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Northwest Institute of Eco Environment and Resources of CAS
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Cold and Arid Regions Environmental and Engineering Research Institute of CAS
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Abstract

The utility model discloses a kind of Wet-dry Meso-micro soil fauna separators, including cabinet (1), it is located at several light heat sources (7) on the cabinet (1) top, it is located at the adjustable partition of the cabinet (1) middle height (3), several separation assemblies for being located on the partition (3) and for the temperature control component by the cabinet (1) internal temperature control in preset temperature range;The separation assembly includes separatory funnel (10), sample holder (9) and animal receiving flask (11), and it is both in cutting ring shape that the sample holder (9), which includes humidogene sample holder and dry raw sample holder,.The utility model separator has two kinds of functions of separating dry space from moist space and temperature automatically controlled function, significantly improves working efficiency in terms of sampling, separation.

Description

A kind of Wet-dry Meso-micro soil fauna separator
Technical field
The utility model relates to soil animal separation technology field more particularly to a kind of Wet-dry Meso-micro soil faunas Separator.
Background technique
Soil animal is the key component in soil ecosystem, they form clast and small food together with microorganism Net controls substance circulation and Nutrient Flow process.And edaphology development is closely related with soil animal isolation technics.
, can be according to different soil animal Individual Sizes and life habit in soil animal separation practice, selection is different Collection separation method.For soil macro-invertebrate (such as spider, myriapod, link class animal and insect larvae), usually make Method, trapping method and large-scale baking worm funnel are picked up with hand to separate;For Meso-micro soil fauna, Individual Size is from 100 microns to several A millimeter mainly includes Nematoda, mite class, projectile tail guiding principle, Symphyla, protocercal tail guiding principle, double tail guiding principles, Pauropoda, centipede, julid and some The lesser insect of individual, usually using dry and wet funnel separator, sucrose centrifuge separation, tray method, trap and floatation etc..
Since Meso-micro soil fauna individual is flat small, isolation technics is always to restrict the difficult point of soil zoology research. For example, sucrose centrifuge separation nematode and the middle-size and small-size arthropod of FLOTATION SEPARATION need to carry out in laboratory, the two sets separation Standby and personnel operating technologies are more demanding, and general laboratory is difficult to meet experiment demand.Dry and wet funnel separator is by artificial Factor influence it is smaller, this be it is a kind of be easy to make, a kind of low and convenient to use method of faking, this device is using simply can be with Batch making can handle large quantities of pedotheques in a short time, the investigation suitable for large scale and big region.However, at present very The design of more separators only has made some improvements only in accordance with principle, has ignored quantity of sampling quantity, disengaging time and soil and withers and falls Influence of the sample properties such as the object sample difference to its separative efficiency, this causes some devices that can not use in other regions.Or When Soil Animals Community system investigation is unfolded, it usually needs a few set equipment (dry and wet funnel separator) are used cooperatively, and expend a large amount of Man power and material.
In addition, overdrying or soil overly moist or Litter-fall sample are all made of the separation of same equipment, can it is excessively high because of temperature and The too fast animal for causing some transfer abilities weak of heating rate, which can not move out, reduces the type and quantity for collecting animal, and then can shadow Ring test result.In recent years, there are also laboratories to use the dry funnel separator of natural air drying, but separation cycle needs one to two It week, can not short-term batch processing gross sample.
Utility model content
The technical problem to be solved by the utility model is to provide a kind of Wet-dry Meso-micro soil fauna separator, It has two kinds of functions of separating dry space from moist space and temperature automatically controlled function, significantly improves working efficiency in terms of sampling, separation.
To solve the above problems, a kind of Wet-dry Meso-micro soil fauna separator described in the utility model, it should Separator includes cabinet, is located at several light heat sources on the cabinet top, is located at the adjustable partition of cabinet middle height, Several separation assemblies for being located on the partition and for controlling the box interior temperature in preset temperature range Temperature control component;
The separation assembly includes separatory funnel, the sample holder being located inside the separatory funnel bucket body and is located at described Animal receiving flask below separatory funnel neck;There is the band mesh plate for supporting the sample holder inside the separatory funnel;
Wherein, the sample holder includes humidogene sample holder and dry raw sample holder, is both contained in cutting ring shape and the humidogene Sample device peripheral wall is equipped with aperture;The separatory funnel is divided into humidogene separatory funnel and dry raw separatory funnel.
Preferably, the separatory funnel is the funnel that filler neck is equipped with water blocking valve, and when use, the water blocking valve closed It closes the separatory funnel to use as humidogene separatory funnel, it is estranged from leakage as doing that the water blocking valve opens the separatory funnel Bucket uses.
Preferably, the separatory funnel is specially glass funnel, and the band mesh plate is glass plate.
Preferably, which further includes sample particle barrier layer, for wrapping up after the sample holder acquires sample Firmly sample.
Preferably, height adjustment plug-in unit is symmetrically arranged on the cabinet wall, the height adjustment plug-in unit vertically opens up There is jack;The partition is inserted in the adjusting that height is realized in the jack of different location by both ends.
Preferably, the temperature control component includes being located at the temperature sensing being located above the partition on the cabinet wall Device, be located at exhaust fan that the lower box is located at below the partition and with the temperature control processor that is both connected;Wherein, The temperature control processor judges the temperature value and preset temperature range for receiving the temperature value from the temperature sensor Relationship, when the temperature value be higher than upper limit value when start the exhaust fan carry out cooling and in the case where received temperature value is lower than again It is turned off automatically when limit value.
Preferably, which further includes the baffle being located at the bottom of box with drainage hole and is located at the row The water container of water receiving is used for below water hole.
Preferably, the animal receiving flask is connected with the neck bottom end of the separatory funnel.
Preferably, the light heat source is specially incandescent lamp bulb, which uses Waterproof lampholder.
Compared with the prior art, the utility model has the following advantages:
1, in the utility model separator, sample holder is in cutting ring shape, is not only sample holder or sampler, such Words can directly acquire the soil or Litter-fall sample, sampler and sample of different depth using sample holder when (1) samples on the spot It does not need to be separated, integrally takes back interior, improve sample collection efficiency, be conducive to batch processing;(2) sample acquisition is adopted using special Sample device is conducive to specification number of samples and depth;(3) guarantee that sample is complete and complete, the sample in animal migration channel when animal separates Product are placed downwards when placing close to earth's surface side, guarantee that soil animal migrates downward into, and avoid causing because of sample broke, extruding The young can not move out.By selecting different samplers that the demand of separation nematode and Small-middle size soil animals may be implemented.
2, the utility model separator is used using special separatory funnel and the matching of different types of sample holder, is had Two kinds of functions of dry and wet funnel realize a tractor serves several purposes, can significantly improve acquisition, separation etc. when in particular for batch processing sample Partial working efficiency.Wherein, it is fixed with band mesh plate inside separatory funnel, on the one hand plays the role of supporting sample holder, separately On the one hand increase the contact area of sample and air or water, isolation soil particle will be native during avoiding soil animal from migrating downward into Earth particle is brought into sample collection bottle.
3, divider height is adjustable in the utility model separator, can quickly adjust the height between sample and light bulb, Convenient for the initial temperature and setting temperature threshold according to sample characteristic adjustment heating.Meanwhile temperature control component can be by box house Temperature controls in preset temperature range, in sample upper and lower surface temperature gradients, migrates downward into conducive to soil animal, improves Separative efficiency.
Detailed description of the invention
Specific embodiment of the present utility model is described in further detail with reference to the accompanying drawing.
Fig. 1 is the schematic diagram of internal structure of separator provided by the embodiment of the utility model.
Fig. 2 is the appearance front view of separator provided by the embodiment of the utility model.
Fig. 3 is the outside side view of separator provided by the embodiment of the utility model.
Fig. 4 is the structural schematic diagram of separatory funnel in separator provided by the embodiment of the utility model.
Fig. 5 is the longitudinal section that raw sample holder is done in separator provided by the embodiment of the utility model.
Fig. 6 is the longitudinal section of humidogene sample holder in separator provided by the embodiment of the utility model.
In figure: 1-cabinet, 2-insulation boards, 3-partitions, 4-height adjustment plug-in units, 5-temperature control processors, 6-air Switch, 7-light heat sources, 8-temperature sensors, 9-sample holders, 10-separatory funnels, 11-animal receiving flasks, 12-air drafts Fan, 13-water containers, 14-drainage holes, 15-band mesh plates, 16-chamber doors, 17-handles, 18-fan housings.
Specific embodiment
With reference to Fig. 1 ~ Fig. 6, the utility model embodiment provides a kind of Wet-dry Meso-micro soil fauna separator, The whole cabinet 1 in cuboid is specifically included, several light heat sources 7 on 1 top of cabinet is located at, it is adjustable to be located at 1 middle height of cabinet Partition 3, the several separation assemblies being located on partition 3 and for by 1 internal temperature control of cabinet in preset temperature range Temperature control component.Wherein, light heat source 7 can specifically use incandescent lamp bulb, which uses Waterproof lampholder;For example, using 25w incandescent lamp bulb, the corresponding separation assembly of a light bulb.
The separator further includes being located at 1 bottom of cabinet to use with the baffle and being located at below drainage hole 14 of drainage hole 14 In the water container 13 of water receiving;When separating hygrocole sample, the water that top is leaked down is collected, steam is reduced and bottom plate is invaded Erosion.
Height adjustment plug-in unit 4 is symmetrically arranged on 1 inner wall of cabinet, height adjustment plug-in unit 4 vertically offers jack;Partition 3 The adjusting that height is realized in the jack of different location is inserted in by both ends.
Above-mentioned separation assembly specifically includes separatory funnel 10, the sample holder 9 being located inside 10 bucket body of separatory funnel and sets Animal receiving flask 11 below 10 neck of separatory funnel;There is the band mesh plate 15 of support sample holder 9 inside separatory funnel 10. Specifically, there is the through-hole for blocking 10 bucket body of separatory funnel on partition 3;The neck bottom end of animal receiving flask 11 and separatory funnel 10 It is directly detachably connected, can accurately and effectively receive the soil animal migrated from top in this way, while also facilitating more Change the preservation liquid in receiving flask or receiving flask.
Wherein, separatory funnel 10 divides for humidogene separatory funnel and dry raw separatory funnel, humidogene sample holder and humidogene when use Separatory funnel matches, and dry raw sample holder is matched with dry raw separatory funnel, replaces and chooses depending on demand 4.
Further, the utility model humidogene separatory funnel and dry raw separatory funnel use same funnel, i.e. separation leakage Bucket 10 is equipped with the funnel of water blocking valve for filler neck, and water blocking valve closes separatory funnel 10 and regards humidogene separatory funnel when use It uses, water blocking valve is opened separatory funnel 10 and used as dry raw separatory funnel;In this case, it is opened when separating different animal species Or water blocking valve is closed, it avoids replacing entire 10 bring troublesome operation of separatory funnel, that is to say, that contain sample by replacement Device can both separate the hygrocoles such as nematode, enchytrae in soil or Litter-fall, and it is dynamic can also to separate the small-sized segmental appendage such as mite and springtail Object, effectively realization a tractor serves several purposes.
In view of when the separation of humidogene soil animal needing that water is added to maintain humidogene environment, therefore separatory funnel 10 is used uniformly glass The waterproofs such as glass or plastic funnel, anti-corrosion material are made, the band mesh plate 15 inside corresponding separatory funnel 10 also for glass plate or Plastic plate.
The utility model sample holder 9 specifically includes humidogene sample holder and dry raw sample holder, is both in cutting ring shape, i.e., this two Kind sample holder is cylindrical and wherein one end has the inclination angle of similar blade, convenient for sampling soil or Litter-fall on the spot Sample.Further, since the separation of humidogene soil animal needs plus water maintains humidogene environment, therefore, humidogene sample holder peripheral wall is equipped with For the aperture intake.Further, it can also be used using sample particle barrier layer cooperation sample holder 9, sample particle barrier Layer stops sample particle to enter for wrapping sample (soil animal can be with free migration) after sample holder 9 acquires sample Animal receiving flask 11;Correspondingly, sample holder 9 has outside annular protrusion far from the one end at inclination angle, hinders convenient for sample particle Interlayer completes the fixation after sample package.
In practical applications, above-mentioned sample particle barrier layer can use nylon wire, can be with for humidogene soil animal One layer of interior lining conventional face tissue, can be with one layer of interior lining conventional hospital gauze, face tissue and yarn for dry raw soil animal Cloth further can effectively prevent loose soil slide-in glass funnel lower part from entering in sample collection bottle.Need exist for explanation It is that these soil animals to be separated are that nylon wire living, above-mentioned, face tissue and gauze do not stop them downward to move It moves.
In the utility model separator, temperature control component specifically includes the temperature for being located at and being located at 3 top of partition on 1 inner wall of cabinet Spend sensor 8, be located at exhaust fan 12 that 1 lower part of cabinet is located at below partition 3 and with the temperature control processor 5 that is both connected; Wherein, temperature control processor 5 is for receiving the temperature value from temperature sensor 8, and judges the temperature value and preset temperature range Relationship, when the temperature value is higher than upper limit value, starting exhaust fan 12 carries out cooling and in received temperature value again lower than lower limit It is turned off automatically when value.
Based on separator disclosed above, method is used in conjunction with practical application and is illustrated.Execute mask work Before, determine that the initial temperature of appropriate sample separation (avoids temperature excessively high or quickly increase to lead to some young by preliminary experiment It is dead), formulate the upper limit of sample separation on this basis, then subtract 5 DEG C as lower limit using the temperature, sample disengaging time according to Depending on the water content and attribute of sample, it usually needs at 48-72 hours.
For the separation of the dry raw soil animal such as mite and springtail: directly being adopted using the special dry raw sample holder of the utility model Collect the soil or Litter-fall sample of different depth;Then the sample holder equipped with sample is wrapped with nylon wire (if it is sandy soil, it can In one layer of hospital gauze of nylon wire liner), number be put into sample collection bag and take back separation, this step can be by sample band It is carried out after returning, saves the outside work time;Dry raw separatory separatory funnel 10 is got out, makes sample closely when placing sample On the band mesh plate 15 of table being placed in separatory funnel 10 on one side, can guarantee animal in this way, migrating channels are smooth in the sample, sample Product can allow soil animal quickly to migrate into funnel close to earth's surface side close to funnel.In the animal receiving flask bottle 11 of funnel bottom Use 75% alcoholic solution as saving liquid.
For the separation of the humidogenes soil animal such as nematode: directly being acquired not using the special humidogene sample holder of the utility model With the soil or Litter-fall sample of depth.Then sample number into spectrum is wrapped into (liner face tissue) with nylon wire and is put into sample acquisition Indoor separation is taken back in bag, this step can carry out after taking back sample, save the outside work time.Get out humidogene separation Separatory funnel 10 makes sample on the band mesh plate 15 of earth's surface being placed in separatory funnel 10 on one side when placing sample, Water is added, water surface elevation is advisable to flood sample.Use water as preservation liquid in the animal receiving flask bottle 11 of funnel bottom.
The utility model Wet-dry Meso-micro soil fauna separator can separate forest, meadow, farmland and desert Meso-micro soil fauna in soil and Litter-fall.Its principle be protected from light using soil animal, To Be Protected from Heat can Litter-fall, the soil body or The principle migrated up and down in water drives soil using incandescent lamp in sample top heated baking formation temperature and intensity of illumination gradient Earth animal migrates eventually fall into sample collecting bottle from up to down, to realize soil animal and soil quick separating.
Disclosed separator based on the above embodiment, another embodiment of the utility model are illustrated for practical application Explanation.
With reference to Fig. 1 ~ Fig. 3, it is in cuboid that the cabinet 1 of the utility model separator is whole, high length and width be 120cm × 130cm × 45cm, interior case includes 12 sets of incandescent lamp bulbs and separation assembly, can handle 12 samples simultaneously.It is upper that cabinet 1, which divides, In lower three parts, middle part has the two split chamber doors 16 of fan, has handle 17 on chamber door 16.
1 upper interior portion of cabinet is fixed with insulation board 2, and insulation board 2 is fixed on bottom plate, there is threading hole on bottom plate, and lamp line is worn It crosses line traffic control and is connected to wiring board above, the corresponding separation assembly of a light bulb;Temperature control processor 5 is located on insulation board 2, with Temperature sensor 8 and exhaust fan 12 connect.
It is provided with air switch 6 in Live wires road in addition, going back, guarantees to isolated protection, and passes through connection inversion Device, solar panel and battery can be used using solar powered or generator powered in power free region, be expanded Use environment and the region of separator are opened up.
At 1 middle part of cabinet, there are 12 circular holes on partition 3, both ends are inserted in the jack of height adjustment plug-in unit 4, between jack Away from for 1cm;The separatory funnel (glass production) that 12 filler necks are equipped with water blocking valve is placed on partition 3 in circular hole;Contain sample 9 volume of device is set as 100cm3, it is highly 5cm;Two temperature sensors 8 are symmetrically fixed on 1 two sides of cabinet, away from sample upper surface height Degree is 5cm, and two exhaust fans 12 are symmetrically positioned in the slightly prominent cabinet in 1 middle and lower part of cabinet, and the part of prominent cabinet is equipped with fan housing 18。
In addition, the box design of the utility model separator reduces the influence that external environment separates sample, Ji Ke Interior can also use in the field environment.The equipment volume is small and exquisite to be readily transported and stores, both can be used Alternating Current Power Supply (power supply or It is generator) again using direct current supply (configuration inverter, battery or solar panel), field is without under exchange electrical environment It can also be used.The equipment solves the limitation in house, parking space and AC power source in soil fauna fact-finding process, sampling It is synchronous with mask work to carry out, the field work time is greatlyd save, sample transportation cost is reduced and improves the rate of departure.
Technical solution provided by the utility model is described in detail above.Specific case pair used herein The principles of the present invention and embodiment are expounded, and the above embodiments are only used to help understand, and this is practical new The method and its core concept of type.It is practical not departing from it should be pointed out that for those skilled in the art Under the premise of new principle, several improvements and modifications can be made to this utility model, these improvement and modification also fall into this In the protection scope of utility model claims.

Claims (9)

1. a kind of Wet-dry Meso-micro soil fauna separator, which is characterized in that the separator includes cabinet (1), if Several light heat sources (7) on the cabinet (1) top, are located at the adjustable partition of the cabinet (1) middle height (3), are located at institute It states several separation assemblies on partition (3) and is used for the cabinet (1) internal temperature control in preset temperature range Temperature control component;
The separation assembly include separatory funnel (10), the sample holder (9) being located inside the separatory funnel (10) bucket body and The animal receiving flask (11) being located at below the separatory funnel (10) neck;Have described in support inside the separatory funnel (10) Band mesh plate (15) of sample holder (9);
Wherein, the sample holder (9) includes humidogene sample holder and dry raw sample holder, both contains sample in cutting ring shape and the humidogene Device peripheral wall is equipped with aperture;The separatory funnel (10) is divided into humidogene separatory funnel and dry raw separatory funnel.
2. separator as described in claim 1, which is characterized in that the separatory funnel (10) is that filler neck is equipped with water blocking The funnel of valve, when use, the water blocking valve closed the separatory funnel (10) as the use of humidogene separatory funnel, the gear Penstock is opened the separatory funnel (10) and is used as dry raw separatory funnel.
3. separator as claimed in claim 2, which is characterized in that the separatory funnel (10) is specially glass funnel, institute Stating band mesh plate (15) is glass plate.
4. separator as described in claim 1, which is characterized in that the separator further includes sample particle barrier layer, is used In the sample holder (9) acquire sample after wrap sample.
5. separator as described in claim 1, which is characterized in that be symmetrically arranged with height adjustment on cabinet (1) inner wall Plug-in unit (4), the height adjustment plug-in unit (4) vertically offer jack;The partition (3) is inserted in different location by both ends The adjusting of height is realized in jack.
6. separator as described in claim 1, which is characterized in that the temperature control component includes being located in the cabinet (1) The temperature sensor (8) being located above the partition (3) on wall is located at the cabinet (1) lower part and is located at below the partition (3) Exhaust fan (12) and with the temperature control processor (5) that is both connected;Wherein, the temperature control processor (5) is come for receiving Temperature value from the temperature sensor (8), and judge the relationship of the temperature value and preset temperature range, when the temperature value is higher than Start the exhaust fan (12) when upper limit value to carry out cooling and closed automatically when received temperature value again is lower than lower limit value It closes.
7. separator as described in claim 1, which is characterized in that the separator further includes being located at the cabinet (1) bottom Portion has the baffle of drainage hole (14) and is located at the water container (13) for being used for water receiving below the drainage hole (14).
8. separator as described in claim 1, which is characterized in that the animal receiving flask (11) and the separatory funnel (10) neck bottom end is connected.
9. separator as described in claim 1, which is characterized in that the light heat source (7) is specially incandescent lamp bulb, the white heat Light bulb uses Waterproof lampholder.
CN201920003067.9U 2019-01-02 2019-01-02 A kind of Wet-dry Meso-micro soil fauna separator Active CN209406785U (en)

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CN201920003067.9U CN209406785U (en) 2019-01-02 2019-01-02 A kind of Wet-dry Meso-micro soil fauna separator

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CN201920003067.9U CN209406785U (en) 2019-01-02 2019-01-02 A kind of Wet-dry Meso-micro soil fauna separator

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109453981A (en) * 2019-01-02 2019-03-12 中国科学院寒区旱区环境与工程研究所 A kind of Wet-dry Meso-micro soil fauna separator

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109453981A (en) * 2019-01-02 2019-03-12 中国科学院寒区旱区环境与工程研究所 A kind of Wet-dry Meso-micro soil fauna separator

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Effective date of registration: 20200724

Address after: 730000 No.318, Donggang West Road, Chengguan District, Lanzhou City, Gansu Province

Patentee after: NORTHWEST INSTITUTE OF ECO-ENVIRONMENT AND RESOURCES, CAS

Address before: 730000 Gansu city of Lanzhou province Donggang West Road No. 320

Patentee before: Institute of environment and Engineering in cold and dry areas, Chinese Academy of Sciences

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