CN209185323U - A kind of explant Aseptic sterilisation cyclotron oscillation device - Google Patents

Abstract

The utility model relates to a kind of explant Aseptic sterilisation cyclotron oscillation devices, including oscillator and disinfectant cup；Oscillator includes closed case and the supporting table being arranged in closed case, the double-deck oscillation frame and ultraviolet lamp, driving motor is provided with below supporting table, driving motor is connect by T mouthfuls of rotary shafts with the bottom driving of the double-deck oscillation frame, is provided with the round saucer for placing disinfectant cup on bilayer oscillation frame.The utility model explant Aseptic sterilisation cyclotron oscillation device has independent sterile working operating room, the entire disinfecting process of explant can be made to be in a gnotobasis and carried out；Oscillation mode is circumnutation, can farthest restore artificial oscillation disinfection mode, disinfection uniformly, thoroughly, mitigate explant bulk damage to the maximum extent；It saves manually, realizes that the automation of medicament oscillation treatment and time, speed is adjustable.

Description

A kind of explant Aseptic sterilisation cyclotron oscillation device

Technical field

The utility model relates to a kind of explant Aseptic sterilisation cyclotron oscillation devices, belong to experiment equipment technical field.

Background technique

Can explant disinfection be very important link in Plant Tissue Breeding, establish regenerating system to species and play certainly It is set for using.Explant is often taken from natural environment or greenhouse, and a large amount of microorganisms and germ are adhered in surface, not only with explant contention Nutrient can also pollute culture medium.Explant is typically passed through disinfectant processing of more than two kinds, time 2s-60min, processing When to improve antivirus effect need continuous oscillation, will be with rinsed with sterile water for several times after every kind of chemicals treatment, process is cumbersome.? In production and experiment, disinfecting process relies primarily on artificial progress, and equipment is mostly used beaker and tweezers, and disinfection is unevenly not thorough, expense When expenses of labour, it is inefficient；It is also easy to leakage explant in disinfection rinse cycle, wastes vegetable material, especially seed, stem apex With stem section is isometric and the lesser test material of weight ratio.

A kind of spray is disclosed now with some chlorination equipments for explant, such as Chinese patent literature CN204191321U Leaching formula Explant sterilizing device, the device include fluid reservoir, conduit, cleaning cabinet and the net box for accessing explant, cleaning Cabinet is connect by conduit with fluid reservoir, and conduit connect place with cleaning cabinet and is provided with spray head, and spray head is located at the inside of cleaning cabinet；Storage Air pump and the first driving device for driving air pump to run are provided at the top of flow container, air pump includes air outlet valve, air outlet valve position In the inside of fluid reservoir；Cleaning cabinet includes top plate, and top plate is provided with through-hole, and net box is inserted in through-hole, the outside socket of net box There is driven wheel, driven wheel is located at the outside of cleaning cabinet, and second for driving driven wheel to rotate is provided at the top of cleaning cabinet and is driven Dynamic device；The bottom of cleaning cabinet is provided with drain pipe, is provided with tapping valve on drain pipe.Chinese patent literature CN102018969A Disclose a kind of plant implant surface sterilization device, the chlorination equipment include: liquid storage bottle, chlorine still, hydrochloric acid instill pipe, Storage cylinder and sterilizing bottle, in which: the first liquid storage bottle is connected with chlorine still, and hydrochloric acid instills the output end of pipe and chlorine occurs Device is connected, and the output end of chlorine still is connected with sterilizing bottle, and the bottom of sterilizing bottle is connected with the input terminal of storage cylinder, storage cylinder Output end be connected with the second liquid storage bottle, the bottom of the first liquid storage bottle and the second liquid storage bottle is connected.Utility model device It is closed, it is ensured that chlorine does not leak；It may be reused after primary generation chlorine, save the cost；The amount of chlorine is kept not in device Become, different vegetable materials can determine suitable disinfecting time by test, convenient for conditions for sterilization accurate quantification.

But although the prior art increases reticular structure or dynamical system to disinfecting container, there are still problems: 1. disappear Malicious container function is single, cannot be changed and select according to explant source, size, cannot be by multiple explants of single treatment Body is effectively isolated to contact to avoid germ and be propagated；2. needing repeatedly to be put into disinfecting container in disinfecting process, taking out disinfection Medicament, process is cumbersome, easily causes secondary pollution to explant and working environment；3. dynamical system (manually or automatically) and explant Shared disinfecting container, easily causes mechanical damage and secondary pollution；4. the explant limited amount of single treatment；5. explant impregnates Time and oscillation rate can not be adjusted；6. some are only applicable to disinfection early periods such as tap water flushing, it is not suitable for disinfectant processing； 7. aseptic processing environment cannot be provided, secondary pollution is easily caused；8. oscillator is designed as moving back and forth or using vibration-type, fortune more Dynamic direction is single, rinsing is insufficient, disinfection is not thorough uneven, easy damaged explant.

Utility model content

In view of the deficiencies of the prior art, the utility model provides a kind of explant Aseptic sterilisation cyclotron oscillation device.

The technical solution of the utility model is as follows:

A kind of explant Aseptic sterilisation cyclotron oscillation device, including oscillator and disinfectant cup；Oscillator includes closed case And supporting table, the double-deck oscillation frame and ultraviolet lamp in closed case are set, it is provided with driving motor below supporting table, is driven Dynamic motor is connect by T mouthfuls of rotary shafts with the bottom driving of the double-deck oscillation frame, is provided on bilayer oscillation frame and is placed disinfectant cup Round saucer.

Preferably, the inner wall of the closed case is each provided with a ultraviolet lamp up and down.The benefit of this design is two purples Outer lamp carries out germicidal irradiation to the lower regions of the double-deck oscillation frame respectively, provides gnotobasis to closed case inside.

Preferably, the double-deck oscillation frame includes the upper layer oscillation frame being vertically connected with and lower layer's oscillation frame, and upper layer vibrates frame It is respectively set that there are four round saucers with lower layer oscillation frame, and four round saucers are evenly arranged on to vibrate frame center as the same of the center of circle On one circumference.The benefit of this design, which is that round saucer is uniformly arranged, can guarantee the double-deck oscillation frame uniform force, carry out oscillation work When industry, stability is more preferable.

Preferably, the disinfectant cup includes cup body, cup lid and locellus sieve plate, and locellus sieve plate is placed in cup body and passes through cup lid Cup body is fastened, cup body inner cavity is divided into multiple independent chambers by locellus sieve plate.

Preferably, it is equipped with a leakage fluid dram at the rim of a cup of the cup body, is equipped with a triangle sieve plate in leakage fluid dram.

Preferably, the leakage fluid dram is triangular pyramidal, and the height of leakage fluid dram is at least the half of cup body height.This sets The benefit of meter is the big leakage fluid dram for selecting triangular pyramidal, is poured out rapidly convenient for liquid.

Preferably, the cup lid includes the arc sections and triangular part of integrally connected.The benefit of this design is that cup lid is fabricated to The shape to match with cup body top shape when being fastened on it in cup body, can cover rim of a cup and leakage fluid dram completely.

Preferably, the locellus sieve plate is cruciform shape locellus sieve plate, and the bottom of cup body inner cavity is provided with locellus sieve Board slot, cruciform shape locellus sieve plate are inserted in locellus sieve plate card slot.

Preferably, triangle sieve plate card slot is provided at the inner wall of the leakage fluid dram, triangle sieve plate is inserted into triangle sieve plate card slot It is interior.

Preferably, the locellus sieve plate, the sieve pore on triangle sieve plate are respectively 5mm, 3mm, 1mm, 100 mesh and 200 mesh.This The benefit of design is, according to different requirement of experiment, to can choose the sieve plate of different sieve pore models, by way of card slot grafting It is conveniently replaceable.

Preferably, the height of the round saucer is the half of cup body height.The benefit of this design is, by disinfectant cup It is placed in round saucer, can guarantee the stabilization of disinfectant cup when oscillation.

Preferably, it is set in the supporting table there are three T mouthfuls of rotary shafts, one of T mouthfuls of rotary shaft is located in supporting table The heart, other two T mouthfuls of rotary shaft are located at the both ends of the same diameter of supporting table, the T mouth rotary shaft and driving motor at supporting table center Output shaft transmission connection.

Preferably, the T mouthfuls of rotary shaft includes connector, upper T mouthfuls of bolt and lower T mouthfuls of bolts, upper T mouthfuls of bolt and T mouthfuls lower Bolt passes through nut respectively and is fixedly connected with connector.

Preferably, the bottom setting of lower layer oscillation frame is there are three C mouthfuls of connection seats, three C mouthfuls of connection seats and three T mouthfuls Rotary shaft connects one to one.

Preferably, the C mouthfuls of connection seat includes opening up in A, the C mouthfuls of connection seat upper ends in C mouthfuls of connection seat upper ends and fixture nut There are C mouthfuls of connection seat grooves, upper T mouthfuls of bolt passes through fixture nut A fitting connection in the C mouth connection seat groove of C mouthfuls of connection seat upper ends Interior, upper T mouthfuls of bolt can rotate in C mouthfuls of connection seat grooves, and the bottom end of upper T mouthfuls of bolt is connect by fixture nut B with connector.

Preferably, the lower T mouthfuls of bolts pass through supporting table, connector and are connect by fixture nut B with connector.

Preferably, transparent plane chamber door is provided with above the supporting table, the cabinet inside below supporting table is equipped with industry control Machine, the surface of cabinet are provided with power switch, adjust the speed knob, time control knob, ultraviolet violet light switch and digital display screen, power switch, Speed regulation knob, time control knob, ultraviolet violet light switch, digital display screen and driving motor connect control with industrial personal computer.This design Benefit is, the enclosure interior above transparent case goalkeeper's supporting table is isolated from the outside out a sterile room, double-layer vibration It swings frame and disinfectant cup is located in this sterile working room, and inner case can be observed by transparent plane chamber door.

The utility model has the beneficial effects that:

1) the utility model explant Aseptic sterilisation cyclotron oscillation device, can will be sterilized using cruciform shape locellus sieve plate Cup inner cavity is divided into mutually independent four chambers, can disposably handle the sterilization operation of a variety of explants, improve disinfection The efficiency of operation.

2) big leakage fluid dram is provided at rim of a cup, liquid is more convenient, while totally enclosed type cup lid is avoided that operation The hand and working environment of personnel causes secondary pollution.

3) the triangle sieve plate at rim of a cup prevents the when of falling liquid explant from missing；Cruciform shape locellus sieve plate is isolated by explant In different spaces, germ contact is avoided to propagate；And triangle sieve plate and cruciform shape locellus sieve plate are Activities Design, can be at any time It is put into and removes, two kinds of sieve plates are equipped with Multiple Type, can be selected according to explant size.

4) the utility model explant Aseptic sterilisation cyclotron oscillation device, disinfectant cup is completely separable with dynamical system, mutually not Interference, will not cause mechanical damage and secondary pollution to explant；Medical fluid oscillation treatment is automatic by being improved to manually, simplifies people Work, and oscillation treatment time and rate are adjustable, oscillation Disinfection Effect is more preferable.

5) the utility model explant Aseptic sterilisation cyclotron oscillation device has independent sterile working operating room, passes through Ultraviolet lamp sterilization provides aseptic processing environment, avoids secondary pollution in treatment process, can make the entire disinfecting process of explant It is in a gnotobasis and carries out；Oscillation mode is circumnutation, can farthest restore artificial oscillation disinfection mode, disappear It is malicious uniform, thorough.

Detailed description of the invention

Fig. 1 is the main view of the utility model Aseptic sterilisation cyclotron oscillation device；

Fig. 2 is disinfectant cup profilograph；

Fig. 3 is the disinfectant cup profilograph with Fig. 2 vertical direction；

Fig. 4 is disinfection cup lid top view；

Fig. 5 is cruciform shape locellus sieve-plate structure schematic diagram；

Fig. 6 is the disinfectant cup top view equipped with triangle sieve plate and locellus sieve plate；

Fig. 7 is the top view of round saucer；

Fig. 8 is the profilograph of the utility model Aseptic sterilisation cyclotron oscillation device；

Fig. 9 is C mouthfuls of connection seat structural schematic diagrams；

Figure 10 is C mouthfuls of connection seat bottom views；

Figure 11 is T mouthfuls of rotation axis structure diagrams；

Figure 12 is T mouthfuls of double helix connected piece structure schematic diagrames；

Figure 13 a is upper T mouthfuls of bolt shape structure schematic diagram I；

Figure 13 b is upper T mouthfuls of bolt shape structure schematic diagram II；

Figure 14 a is lower T mouthfuls of bolt shape structure schematic diagram I；

Figure 14 b is lower T mouthfuls of bolt shape structure schematic diagram II；

Figure 15 is T mouthfuls of rotary shaft top views；

Wherein: 1, disinfectant cup；11, cup lid is sterilized；12, leakage fluid dram；13, triangle sieve plate；14, triangle sieve plate card slot；15, ten Word X-shape locellus sieve plate；16, locellus sieve plate card slot；2, cabinet；21, sterile room；211, machine box door；2111, machine box door Handle；212, ultraviolet lamp；213, the double-deck oscillation frame；2131, upper layer vibrates frame；2132, frame is vibrated in lower layer；214, C mouthfuls of connection seats； 2141, C mouthfuls of connection seat upper ends；2142, C mouthfuls of connection seat grooves；2143, fixture nut A；215, round saucer；216, T mouthfuls of rotations Axis；2161, upper T mouthfuls of bolt；21611, nut A circle bearing；21612, T mouthfuls of double bolt connection part hexagon joining places； 21613, nut B circle bearing；2162, fixture nut B；2163, T mouthfuls of double bolt connection parts；21631, hexagonal connection holes； 2164, lower T mouthfuls of bolt；21641, nut B circle bearing；21642, T mouthfuls of double bolt connection part hexagon joining places；21643, Nut B circle bearing；21644, supporting table circle junction；217, supporting table；22, dynamical system and actuation member；221, Adjust the speed knob；2211, rotational speed regulation element；222, time control knob；2221, time control components；223, digital display screen；2231, Digital display element；224, ultraviolet violet light switch；2241, ultraviolet violet light switch element；225, power switch；2251, power switch element； 226, motor.

Specific embodiment

The utility model is described further by way of example and in conjunction with the accompanying drawings, but not limited to this.

Embodiment 1:

As shown in Figure 1, the equipment mainly includes vibration the present embodiment provides a kind of explant Aseptic sterilisation cyclotron oscillation device Swing device and disinfectant cup 1；Oscillator includes closed case 2 and the supporting table being arranged in closed case 217, the double-deck oscillation frame 213 and ultraviolet lamp 212, the lower section of supporting table 217 is provided with driving motor 226, driving motor 226 by T mouthfuls of rotary shafts 216 with The bottom driving connection of bilayer oscillation frame 213, bilayer vibrate the round saucer 215 for being provided on frame 213 and placing disinfectant cup 1.

Specifically, as shown in Figures 2 to 6, disinfectant cup 1 includes that cup lid 11, leakage fluid dram 12, triangle sieve plate 13 and cross are handed over Fork-shaped locellus sieve plate 15.Disinfection 11 one end of cup lid is arc sections, one end is triangular part, and triangular part and arc sections are integrally formed knot Structure, convenient for cup body and big leakage fluid dram 12 are all sealed, liquid spluttering when preventing oscillation treatment.Big leakage fluid dram 12 is pyrometric cone Shape, the highly half for cup body height, are discharged rapidly convenient for liquid.Big leakage fluid dram 12 is equipped with three with cup body joining place inside Angle sieve plate card slot 14 is connected close with cup body with fixed triangle sieve plate 13, triangle sieve plate 13 for being put into, removing；Cup body bottom The inside is equipped with locellus sieve plate card slot 16, for being put into, removing and fixed cross X-shape locellus sieve plate 15, cruciform shape locellus Sieve plate 15 is connected close with cup body.Triangle sieve plate 13 and cruciform shape locellus sieve plate 15 are equipped with sieve pore above, are convenient for liquid flow Out；Two kinds of sieve plates are equipped with different model according to slot size, and the aperture on different model sieve plate is respectively 5mm, 3mm, 1mm or sieve Hole number is 100 mesh, 200 mesh, can select sieve plate model according to explant size；Cruciform shape locellus sieve plate 15 will once be located Multiple explants of reason separate in 4 different spaces, play the role of that explant is isolated, germ contact is avoided to propagate.

As shown in figure 8, the double-deck oscillation frame 213 includes that frame 2132 is vibrated in upper layer oscillation frame 2131 and lower layer, it is bilevel It vibrates frame to connect by the vertical plate of two sides, upper layer and lower layer vibrate respectively there are four round saucer 215 on frame, and disinfectant cup 1 is placed on circle In shape saucer 215, fixed disinfectant cup 1 when playing the role of oscillation, round saucer height is the half of cup body height, every layer Four round saucers 215 be evenly arranged on using bilayer oscillation 213 center of frame as on the same circumference in the center of circle；Frame 2132 is vibrated in lower layer 3 C mouthfuls of connection seats 214, including C mouthfuls of connection seat upper ends 2141 and fixture nut A 2,143 two are fixed on the central axes of bottom Point, 3 C mouthfuls of 214 equidistantly distributeds of connection seat.

The crate part of 217 top of supporting table is divided into an independent sterile room 21, and is furnished with special transparent case Door 211, for internal aseptic operating environment and external environment is isolated；Two sterilizations are fixed on 21 rear side inner wall of sterile room With ultraviolet lamp 212.3 T mouthfuls of rotary shafts, 216,3 T mouthfuls of rotary shafts 216 are equipped in supporting table 217 altogether and are located at 217 axis of supporting table Line, for corresponding rotation 3 C mouthfuls of connection seats 214 of connection, one of T mouthfuls of rotary shaft 216 is located in supporting table 217 The heart, other two T mouthfuls of rotary shaft 216 are located at the both ends of the same diameter of supporting table 217, the T mouth rotary shaft 216 at supporting table center with The output shaft of driving motor 226 is sequentially connected.T mouthfuls of rotary shafts 216 include upper T mouthfuls of bolt 2161, lower T mouthfuls of bolt 2164, T mouthfuls pairs Bolt connection part 2163 and 3 piece fixture nut B 2162.C mouthfuls of connection seats 214 are T mouthfuls upper for the upper T mouthfuls of bolts 2161 of chimeric connection Bolt 2161 can rotate in C mouthfuls of connection seat grooves 2142.Lower T mouthfuls of bolts 2164 are revolved T mouthfuls by fixture nut B 2162 Shaft 216 is fixed on supporting table 217 and prevents from being detached from below, can make spinning motion after energization.Upper T mouthfuls of bolt 2161, lower T mouthfuls of bolt 2164 are fixedly connected by T mouthfuls of double helix connectors 2163；Respectively there are a hexagonal connection holes in T mouthfuls of 2163 two sides of double helix connector 21631；Upper T mouthfuls of bolt 2161, lower T mouthfuls of bolt 2164 and fixture nut A2143,2162 coupling part 21611 fixture nut B, 21641,21643 be circle, is hexagon with 21631 coupling part 21612,21642 of hexagonal connection holes.

Cabinet door handle 2111 is equipped on the outside of machine box door 211, convenient for switch machine box door.There is speed regulation knob in 2 outer surface of cabinet 221, time control knob 222, ultraviolet violet light switch 224, power switch 225 and digital display screen 223, it is internal respectively with rotational speed regulation element 2211, time control components 2221, power switch element 2251, ultraviolet violet light switch element 2241 are connected with digital display element 2231, It finally connect and is controlled by it with the industrial personal computer in cabinet, specific connection line can be realized according to routine techniques；2 inside electricity of cabinet Machine 226 is engaged connection with the T mouth rotary shaft 216 in the middle part of supporting table, T mouthfuls of 216 lower part rotations of rotary shaft after energization, T mouthfuls in drive Bolt 2161 is circled around motor axis；C mouthfuls of connection seats 214 in 3 be actively coupled chimeric with upper T mouthfuls of bolt 2161, phase It is circled around lower T mouthfuls of bolt 2164 with answering, and then the double-deck oscillation frame is driven to make circumnutation, to realize on oscillation frame Disinfectant cup simulation manpower rotates back and forth the purpose for rocking disinfection.

Embodiment 2:

The present embodiment provides a kind of explant application method of Aseptic sterilisation cyclotron oscillation device, oscillator used is to implement Technical solution described in example 1, the specific operation process is as follows:

(1) explant disinfection pretreatment: select size for 2cm or so, have the pomegranate stem section explant 32 of the full bud of stalwartness It is a, it is put in be placed under water tap in leakage basket and rinse, impregnate 10min with 0.5% commercially available dish washing liquid solution, flowing water washes down；Nothing Bacterium water rinses 2 times；

(2) disinfectant cup and sterile room with preceding antivirus: select the triangle sieve plate 13 and cruciform shape locellus of 5mm model Sieve plate 15 is installed in eight disinfectant cups 1 in corresponding triangle sieve plate card slot 14 and locellus sieve plate card slot 16, is put into cabinet 2 pairs In eight round saucer 215 of layer oscillation frame 213, machine box door 211 is closed, 225 and ultraviolet violet light switch 224 are turned on the power switch, The ultraviolet lamp disinfection time is adjusted by time control knob 222 as 40min；Disinfectant cup 1 is taken out after 40min rapidly and shuts machine box door 211；

(3) divide and put explant, the first thimerosal is added: in superclean bench, being put into each locellus of disinfectant cup 1 1 explant；The alcohol for pouring into half 75% in eight disinfectant cups respectively, buckles lid 11；

(4) timing constant speed oscillation treatment in sterile room: eight disinfectant cups 1 are placed again into the round saucer 215 of cabinet 2 And close machine box door 211；The power switch 225 on cabinet 2 is opened, is 25s by 222 adjustment time of time control knob, passes through tune It is 180r/min that fast knob 221, which adjusts revolving speed,；Pass through 223 cross-check information of digital display screen；Frame 213 is vibrated after 25s stops convolution fortune It is dynamic；Machine box door 211 is opened, disinfectant cup 1 is removed rapidly and shuts machine box door 211, cup lid 11 is opened in superclean bench, by wine Essence is poured into returnable, and triangle sieve plate 13 and cruciform shape locellus sieve plate 15 effectively prevent stem section to flow out with alcohol；? Enter aseptic water washing 2 times；

(5) second of thimerosal and oscillation treatment is added: in superclean bench, being poured into respectively in eight disinfectant cups 1 10% NaCl0 covers lid 11；It repeats step (4), unlike: 6min will be adjusted to the time, revolving speed is adjusted to 150r/ Min, aseptic water washing number are 5 times；

(6) explant is seeded to culture medium: eight disinfectant cups 1 is finally removed, in superclean bench with more sterile tweezer Son successively presss from both sides out stem section in turn, is respectively put into moist heat sterilization, is covered in the culture dish of filter paper, not contact between explant, uses Scalpel cuts redundance, is sized, and is inoculated in through in moist heat sterilization, the suitable culture medium of ingredient.

Embodiment 3:

The present embodiment provides a kind of explant application method of Aseptic sterilisation cyclotron oscillation device, oscillator used is to implement Technical solution described in example 1, the specific operation process is as follows:

(1) explant disinfection pretreatment: the tomato seeds of 8 parts of 1g (total 8g) without mildew are weighed, are placed in the 100 mesh string bags It is rinsed under water tap, impregnates 15min with 1% commercially available dish washing liquid solution, flowing water washes down；Aseptic water washing 2 times；

(2) disinfectant cup and sterile room with preceding antivirus: select the triangle sieve plate 13 and cruciform shape point of 100 mesh models Room sieve plate 15 is installed in disinfectant cup 1 in corresponding triangle sieve plate card slot 14 and locellus sieve plate card slot 16, and it is double-deck to be put into cabinet 2 It vibrates in eight round saucers 215 of frame 213, closes machine box door 211, turn on the power switch 225 and ultraviolet violet light switch 224, lead to It crosses time control knob 222 and adjusts the ultraviolet lamp disinfection time as 30min；Disinfectant cup 1 is taken out after 30min rapidly and shuts machine box door 211；

(3) divide and put explant, the first thimerosal is added: in superclean bench, putting 1 in each disinfectant cup 1 and especially plant Body is put into 1/4 especially implant in that is, each locellus, pours into the alcohol of half 75% in 8 disinfectant cups 1 respectively, cover lid 11；

(4) timing constant speed oscillation treatment in sterile room: eight disinfectant cups 1 are placed again into the round saucer 215 of cabinet 2 And close machine box door 211；The power switch 225 on cabinet 2 is opened, is 25s by 222 adjustment time of time control knob, passes through tune It is 200r/min that fast knob 221, which adjusts revolving speed,；Pass through 223 cross-check information of digital display screen；Frame 213 is vibrated after 25s stops convolution fortune It is dynamic；Machine box door 211 is opened, disinfectant cup 1 is removed rapidly and shuts machine box door 211, cup lid 11 is opened in superclean bench, by wine Essence is poured into returnable, and triangle sieve plate 13 and cruciform shape locellus sieve plate 15 effectively prevent seed to flow out with alcohol；? Enter aseptic water washing 2 times；

(5) second of thimerosal and oscillation treatment is added: in superclean bench, pouring into 10% respectively in 8 disinfectant cups 1 NaCl0, cover lid 11；It repeats step (4), unlike: 7min will be adjusted to the time, revolving speed is adjusted to 180r/min, Aseptic water washing number is 5 times；

(6) explant is seeded to culture medium: 8 disinfectant cups is removed, in superclean bench with more sterile metal spoon Seed is successively scraped in turn, moist heat sterilization is respectively put into, is covered in the culture dish of filter paper, and each locellus seed will be separated and be put, and be blotted It is inoculated in after moisture through in moist heat sterilization, the suitable culture medium of ingredient.

Claims (9)

1. a kind of explant Aseptic sterilisation cyclotron oscillation device, which is characterized in that including oscillator and disinfectant cup；Oscillator includes Closed case and the supporting table being arranged in closed case, bilayer vibrate frame and ultraviolet lamp, are provided with drive below supporting table Dynamic motor, driving motor are connect by T mouthfuls of rotary shafts with the bottom driving of the double-deck oscillation frame, are provided with placement on bilayer oscillation frame The round saucer of disinfectant cup.

2. explant as described in claim 1 Aseptic sterilisation cyclotron oscillation device, which is characterized in that the double-deck oscillation frame packet The upper layer oscillation frame being vertically connected with and lower layer's oscillation frame are included, upper layer oscillation frame and lower layer's oscillation frame are respectively set there are four circular cup Support, and four round saucers are evenly arranged on to vibrate frame center as on the same circumference in the center of circle.

3. explant as described in claim 1 Aseptic sterilisation cyclotron oscillation device, which is characterized in that the disinfectant cup includes cup Body, cup lid and locellus sieve plate, locellus sieve plate are placed in cup body and fasten cup body by cup lid, and locellus sieve plate separates cup body inner cavity For multiple independent chambers.

4. explant as claimed in claim 3 Aseptic sterilisation cyclotron oscillation device, which is characterized in that at the rim of a cup of the cup body Equipped with a leakage fluid dram, a triangle sieve plate is equipped in leakage fluid dram.

5. explant as claimed in claim 3 Aseptic sterilisation cyclotron oscillation device, which is characterized in that the locellus sieve plate is ten Word X-shape locellus sieve plate is provided with locellus sieve plate card slot in the bottom of cup body inner cavity, and cruciform shape locellus sieve plate is inserted in point In the sieve plate card slot of room.

6. explant as claimed in claim 2 Aseptic sterilisation cyclotron oscillation device, which is characterized in that the supporting table is equipped with Three T mouthfuls of rotary shafts, one of T mouthfuls of rotary shaft are located at the center of supporting table, and it is same that other two T mouthfuls of rotary shaft is located at supporting table The both ends of one diameter, the T mouth rotary shaft at supporting table center and the output shaft of driving motor are sequentially connected；

The T mouthfuls of rotary shaft includes connector, upper T mouthfuls of bolt and lower T mouthfuls of bolts, and upper T mouthfuls of bolt and lower T mouthfuls of bolts pass through respectively Nut is fixedly connected with connector；

There are three C mouthfuls of connection seats for the bottom setting of lower layer's oscillation frame, and three C mouthfuls of connection seats and three T mouthfuls of rotary shafts are one by one It is correspondingly connected with.

7. explant as claimed in claim 6 Aseptic sterilisation cyclotron oscillation device, which is characterized in that the C mouthfuls of connection seat packet It includes and offers C mouthfuls of connection seat grooves in A, the C mouthfuls of connection seat upper ends in C mouthfuls of connection seat upper ends and fixture nut, upper T mouthfuls of bolt passes through solid Determine nut A to be fitting to connection in the C mouth connection seat groove of C mouthfuls of connection seat upper ends, upper T mouthfuls of bolt can be in C mouthfuls of connection seat grooves The bottom end of rotation, upper T mouthfuls of bolt is connect by fixture nut B with connector.

8. explant as claimed in claim 6 Aseptic sterilisation cyclotron oscillation device, which is characterized in that the lower T mouthfuls of bolt is worn It crosses supporting table, connector and is connect by fixture nut B with connector.

9. explant as described in claim 1 Aseptic sterilisation cyclotron oscillation device, which is characterized in that the top of the supporting table It is provided with transparent plane chamber door, the cabinet inside below supporting table is equipped with industrial personal computer, and the surface of cabinet is provided with power switch, speed regulation Knob, time control knob, ultraviolet violet light switch and digital display screen, power switch, speed regulation knob, time control knob, ultraviolet violet light switch, digital display Screen and driving motor connect control with industrial personal computer.