CN209069862U - Mass Spectrometer Method device and its optical system - Google Patents
Mass Spectrometer Method device and its optical system Download PDFInfo
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- CN209069862U CN209069862U CN201821568695.3U CN201821568695U CN209069862U CN 209069862 U CN209069862 U CN 209069862U CN 201821568695 U CN201821568695 U CN 201821568695U CN 209069862 U CN209069862 U CN 209069862U
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Abstract
The utility model relates to a kind of Mass Spectrometer Method device and its optical systems.The laser of laser cell uses solid state laser in optical system, it is very small that the axis direction of the incident direction of laser and ion source cavity is formed by angle, the initial velocity direction for the particle that laser ionization generates is substantially the central axis direction diverging along ion source cavity, therefore the initial kinetic energy diverging of ion is smaller, is conducive to the resolution ratio and sensitivity that improve Mass Spectrometer Method device.And to be formed by angle also very small for the axis direction of illumination light and imaging optical path and ion source cavity that lighting unit issues, and illumination and imaging direction are full symmetric, thus lighting source can achieve maximization in the crossover range of the sample target plate surface irradiated area generated and the area of imaging unit imaging, it both can be to avoid the generation of evil shadow effect, the range of imaging can be greatly improved again, and then image quality greatly improves.
Description
Technical field
The utility model relates to Mass Spectrometer Method instrument fields, more particularly, to a kind of Mass Spectrometer Method device and its optical system
System.
Background technique
The appearance of substance assistant laser desorpted (MALDI) ion source provides very important skill for macromolecular complete analysis
Art means.MALDI technology is very suitable to be combined with flight time mass spectrum (TOFMS) detection technique, this is also MALDI technology
Most successful technical combinations.In recent years, with laser technology, high-speed data acquisition, ion detection, matrix technology quick hair
Exhibition, the performance of MALDI ionization techniques have also obtained promotion at full speed, so that modern MALDI instrument has had been provided with high-resolution
The even quantitative ability of rate, high sensitivity, high quality range.In recent years, MALDI-TOFMS be increasingly becoming protein, polypeptide,
The important means of the large biological molecules such as nucleic acid analysis.It is composed using the protein Ion peak figure that MALDI-TOFMS draws microorganism, with
The mass spectrometric data of Clinical microorganism is compared with standard protein fingerprints database afterwards, so that it may reach microbial identification
Purpose.Compared to the microbial identifications technology such as traditional biochemical method, luminescence method, this method is either in identification speed, knot
Fruit accuracy rate or technical costs, personnel operate requirement etc. all with certain advantage.The height that MALDI-TOFMS has
Sensitivity, wide mass range and the technical characterstics such as moderate resolution ratio and Mass accuracy, it is in addition easy to operate, quick, economical
Etc. handling characteristics, become high-throughput, business and run most potential instrument.It is led in microbial identification and detection of nucleic acids
Domain, MALDI-TOFMS have moved towards the clinical application stage.
MALDI-TOFMS needs the sample material being analysed to mix titration on stainless steel target plate with matrix, mixes
It closes object to air-dry or generate co-crystal thereof after drying up, by the way that the laser point after focusing is irradiated to co-crystal thereof surface, base
Matter transfers energy to sample molecule to be analyzed after absorbing laser energy, so that sample molecule is ionized, it is then sharp
It is detected with time of-flight mass spectrometer, and the corresponding substance of each ion is identified according to the difference of different ions flight time.
In sample analysis, since distribution of the co-crystal thereof on target plate surface of generation is often uneven, the crystalline solid shape of some positions
State is preferable, and the crystalline material of formation is more, and some positions do not form crystalline material then.Therefore laser sample analysis is being carried out
During, operator is required to see the crystallization quality of sample at the crystalline state of sample and different target position in real time,
To select most suitable analysis site to be tested.Since laser light path system is often very long, slight vibration or displacement will
Lead to the center of laser point offset sample to be analysed, therefore the location of observation laser sample point being capable of effective monitor in real time
The working condition of device, to guarantee the performance of the performance of instrument.In addition, for different analysis samples, the color of crystalline solid,
Reflectance, shape etc. are all inconsistent, also inconsistent for the sensitivity of light, therefore imaging device must also be able to adapt to difference
The illumination and imaging demand of analyte.
However, traditional most of Mass Spectrometer Method equipment is still mainly using nitrogen molecular laser (N2 laser) as ionization
Source, mass resolution and image quality are to be improved.
Utility model content
Based on this, it is necessary to provide a kind of Mass Spectrometer Method device and its light that can be improved mass resolution and image quality
System.
The technical solution that the utility model solves the technical problem is as follows.
A kind of optical system of Mass Spectrometer Method device, including laser cell, lighting unit, imaging unit and optical reflection
Mechanism；The laser cell, the lighting unit and the imaging unit are set to the ion source chamber of the Mass Spectrometer Method device
The outside of body, the optical reflection mechanism are set to the inside of the ion source cavity；
The laser of the laser cell is solid state laser, and the laser that the laser cell issues is through the optical reflection
It is mapped to sample surfaces after mechanism reflection and the laser beam of reflection and the angle of the axis perpendicular to sample target plate are greater than 0 ° and not
Greater than 10 °；The illumination light that the lighting unit issues is irradiated to sample surfaces after optical reflection mechanism reflection and through institute
The angle of illumination light light after stating the reflection of optical reflection mechanism and the axis perpendicular to sample target plate is greater than 0 ° and is not more than
10°；The imaging unit is used to receive the illumination light reflected by the sample to be imaged for sample.
In one of the embodiments, the laser cell include optics fixed platform and be set to the optics fixed platform
On the solid state laser, light path system and shell；
The light path system includes the first condenser lens, collimation lens and the second condenser lens, the solid state laser hair
Laser beam out generates the laser beam of diverging through first condenser lens, and the laser beam of the diverging is after the collimation lens
As the laser beam of collimation, the laser beam of the collimation can be through the optical reflection machine after second condenser lens focusing
It is incident on sample after structure reflection；
First condenser lens, the collimation lens and second condenser lens are disposed in the housing, and institute
It is adjustable to state the distance between the first condenser lens and the collimation lens.
The light path system further includes optical filter in one of the embodiments, and the optical filter is for adjusting laser beam
Energy.
The shell is modular construction, including sequentially connected first lens housing mould in one of the embodiments,
Block, the second lens housing module and the third lens housing module；First condenser lens, the collimation lens and described
Two condenser lenses are respectively arranged on the first lens housing module, the second lens housing module and the third lens shell
In module；
It is can be movable relatively between the first lens housing module and the second lens housing module to adjust described
The distance between one condenser lens and the collimation lens.
In one of the embodiments, the shell further include the first reflection housing module, second reflection housing module and/
Or third reflects housing module, correspondingly, the light path system further includes first in the first reflection housing module
Reflecting mirror reflects the second reflecting mirror in housing module set on described second and/or is set in third reflection housing module
Third reflecting mirror；
The first reflection housing module is connected to the first lens housing module and the second lens housing module
Between, the second reflection housing module be connected to the second lens housing module and the third lens housing module it
Between, the third reflection housing module is connected to after the third lens housing module.
Structure is connected through a screw thread between adjacent housing module in one of the embodiments, to link together；
Relative motion is by spirally adjusting between the first lens housing module and the second lens housing module
What section structure was adjusted.
The laser cell further includes optical filtering regulating mechanism, lens adjusting mechanism and reflection in one of the embodiments,
At least one regulating mechanism in regulating mechanism；
The optical filtering regulating mechanism is connect with the optical filter, for driving the optical filter to rotate；
The lens adjusting mechanism is connect with the first lens housing module and/or the second lens housing module,
With the relative position for adjusting the first lens housing module Yu the second lens housing module；
The Reflective regulation mechanism and first reflecting mirror, second reflecting mirror and/or the third reflecting mirror connect
It connects with the angle for adjusting corresponding reflecting mirror.
The optical reflection mechanism tool is respectively used to there are three reflecting surface by incident institute in one of the embodiments,
It states laser reflection and reflexes to the sample and sample is anti-to the sample, by the illumination light that the lighting unit issues
The illumination light penetrated reflexes to the imaging unit.
The imaging unit includes optical lens and filming apparatus in one of the embodiments, by the optical reflection
The illumination light that mechanism reflects takes in the filming apparatus after capturing via the optical lens.
A kind of Mass Spectrometer Method device, including optical system described in ion source component and any of the above-described embodiment；It is described
Ion source component includes ion source cavity, sample target plate and pole piece component, and the sample target plate and the pole piece component are set to
Lower section in the ion source chamber body and positioned at the optical reflection mechanism；The laser cell is located at the ion source cavity
Side.
The study found that influence of the initial characteristic for instrument overall performance for the ion that laser ionization process generates is to Guan Chong
It wants, direct inverse relationship is presented in the initial flight direction of the ion of generation and the direction of laser light incident.Therefore, for theoretically
The ion that laser vertical irradiation sample generates will be dissipated along the axis of instrument, most beneficial for raising resolution ratio.However, traditional
Angle between the laser incident angle and axial direction of most equipment is excessive, and the ion generated diverging direction is caused to deviate axis,
To influence mass resolution.Further study show that since traditional MALDI-TOFMS ion source structure is very compact, it is real
Now there is certain difficulty to the illumination of sample spot and imaging, entire optical system needs a series of groups across ion source system
Part, therefore, the large angle illumination and imaging that traditional mass spectroscopy device generally uses.On the one hand this method will lead to the figure of imaging
It as generating more serious oblique shadow effect, causes the deformation extent that obtained figure is imaged larger, generally requires that do figure whole
Shape processing, it is complex；On the other hand, since illumination and imaging optical path system are frequently not same optical path, the friendship of the two
The region area that converges is smaller, causes the areal extent being ultimately imaged limited.
Based on this, for laser incident angle, illumination and imaging angle present in traditional MALDI-TOFMS instrument
Excessive problem, the utility model provide a kind of low-angle laser light incident, illumination and the Mass Spectrometer Method device of imaging and its
Optical system.Wherein, the laser that laser cell issues is mapped to the laser of sample surfaces and reflection after the reflection of optical reflection mechanism
Light with perpendicular to sample target plate axis angle be greater than 0 ° and be not more than 10 °, in this way due to the incident direction of laser with from
The axis direction of component cavity is formed by that angle is very small, and the initial velocity direction for the particle that laser ionization generates is substantially edge
The central axis direction of ion source cavity dissipates, therefore the initial kinetic energy diverging of ion is smaller, is conducive to improve Mass Spectrometer Method device
Resolution ratio and sensitivity.And the illumination light that lighting unit issues is irradiated to sample surfaces after the reflection of optical reflection mechanism and through light
The angle of illumination light light after learning reflecting mechanism reflection and the axis perpendicular to sample target plate is greater than 0 ° and no more than 10 °, at
As unit is used to receive by the illumination light of sample reflection, the angle of such imaging optical path is also very small.And illumination and imaging direction
It is full symmetric, thus the lighting source area that is imaged with imaging unit in the irradiated area that sample target plate surface generates intersects model
It encloses and can achieve maximization, not only can be to avoid the generation of evil shadow effect, but also the range of imaging can be greatly improved, and then at image quality
Amount greatly improves.
Further, above-mentioned Mass Spectrometer Method device and its optical system use the mass ion source based on solid state laser and set
Meter is compared to the advantages that N2 laser is long with the service life, and the burst length is short, compact, high repetition frequency, helps to improve
Mass Spectrometer Method device resolution ratio and the frequency for reducing maintenance.
Especially, which is consolidated by the way that solid state laser, entire light path system and shell are completely fixed in optics
On fixed platform, light path system is located in shell, and structure is highly stable, and convenient for installation and maintenance.When in use, adjusting can be passed through
The distance between first condenser lens and collimation lens realize the adjusting to laser facula size, easy to use.
Further, in order to reduce the angle in laser light incident direction Yu mass spectrum tof tube axis, entire ion source as far as possible
Other pole piece components of component etc. are all mounted on the lower section of optical reflection mechanism, and the design of pole piece component inner hole can guarantee
Laser and illumination light can smoothly reach sample surfaces without the influence by pole piece component.Therefore, swashing after reflecting
Light can reach sample surfaces with very small angle, and by sample ionization, the area source for illuminating generation can be by pole piece component
Intermediate hole array reaches imaging unit.
Detailed description of the invention
Fig. 1 is the partial structure diagram of the Mass Spectrometer Method device of an embodiment of the present invention；
Fig. 2 is the structural schematic diagram of Fig. 1 intermediate ion source component and partial optical system；
Fig. 3 is the structural schematic diagram of laser cell in Mass Spectrometer Method device shown in Fig. 1；
Fig. 4 is the cross-sectional view of laser cell shown in Fig. 3；
Fig. 5 is the light path principle schematic diagram of laser cell shown in Fig. 3；
Fig. 6 is the displacement of the first condenser lens and the relation curve of focus point spot diameter；
Fig. 7 is actual facula measurement result；
Fig. 8 is the structural schematic diagram of the laser cell of another embodiment.
Specific embodiment
The utility model is more fully retouched below with reference to relevant drawings for the ease of understanding the utility model,
It states.The preferred embodiment of the utility model is given in attached drawing.But the utility model can come in many different forms in fact
It is existing, however it is not limited to embodiment described herein.On the contrary, purpose of providing these embodiments is makes public affairs to the utility model
The understanding for opening content is more thorough and comprehensive.
It should be noted that be referred to as " being set to " another element when element, it can directly on the other element or
There may also be elements placed in the middle by person.When an element is considered as " connection " another element, it can be directly to
Another element may be simultaneously present centering elements.
Unless otherwise defined, all technical and scientific terms used herein are led with the technology for belonging to the utility model
The normally understood meaning of the technical staff in domain is identical.Terminology used in the description of the utility model herein only be
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term as used herein "and/or" includes
Any and all combinations of one or more related listed items.
It please join Fig. 1, an embodiment of the present invention provides a kind of Mass Spectrometer Method device 10 comprising ion source component
100 and optical system 200.Mass Spectrometer Method device 10 can be all kinds of Mass Spectrometer Method devices such as time of-flight mass spectrometer.
As shown in Fig. 2, ion source component 100 includes ion source cavity 110, sample target plate 120 and pole piece component 130.
Ion source cavity 110 has a vacuum chamber 112.In a specific example, the top of ion source cavity 110 is in
Octahedral column structure, be preferably positive octagon column body structure.
Sample target plate 120 and pole piece component 130 are located in the vacuum chamber 112 of ion source cavity 110.Sample target plate 120 is used
In the co-crystal thereof sample for placing sample material and matrix to be analyzed.Pole piece component 130 be used for by sample ionization generate from
Son imports quality detection device and carries out quality analysis.The extraction pole piece 132 of pole piece component 130 is located at the top of sample target plate 120,
And offer the pole piece inner hole 134 passed through for the light of optical system.
It please join Fig. 1, optical system 200 includes that laser cell 210, lighting unit 220, imaging unit 230 and optics are anti-
Penetrate mechanism 240.Laser cell 210, lighting unit 220 and imaging unit 230 are set to the outside of ion source cavity 110, optics
Reflecting mechanism 240 is set to the inside of ion source cavity 110.
Incorporated by reference to Fig. 3, Fig. 4 and Fig. 5, laser cell 210 includes optics fixed platform 211, solid state laser 212, optical path
System 213 and shell 214.Solid state laser 212, light path system 213 and shell 214 are set in optics fixed platform 211.Optics
Fixed platform 211 is fixed on the side of ion source cavity 110.
Solid state laser 212 is fixed in optics fixed platform 211.In a specific example, solid state laser 212 is sent out
The wavelength for the laser penetrated is 343nm.Solid state laser 212 compares more traditional N2 laser, has long service life, laser arteries and veins
The advantages that width is small, repetition rate is high, small in size is rushed, point of substance assistant laser desorpted time of-flight mass spectrometer can be significantly improved
Resolution, reduces the frequency of maintenance, and is conducive to the volume Miniaturization Design of instrument.
Light path system 213 includes the first condenser lens 2131, collimation lens 2132 and the second condenser lens 2133.Solid swashs
The laser beam that light device 212 issues generates the laser beam of diverging, the collimated lens of the laser beam of diverging through the first condenser lens 2131
Become the laser beam of collimation after 2132, the laser beam of collimation can be by optical reflection mechanism after the focusing of the second condenser lens 2133
240 are reflected on sample.First condenser lens 2131, collimation lens 2132 and the second condenser lens 2133 are set to shell 214
It is interior, and the first condenser lens 2131 and the distance between collimation lens 2132 are adjustable.
Shown in fig. 5 is the schematic illustration of entire light path system 10.Solid state laser 212 and the first condenser lens 2131
The distance between (F1, focal length f1 in figure) is L1, and the laser pulse that solid state laser 212 generates focuses saturating by first
Mirror 2131 generates the light beam of diverging, and having collimation lens 2132 at the rear certain distance L 2 of this light beam, (F2, focal length are in figure
F2) for collimating the laser beam of diverging, there is the second condenser lens 2133 (in figure at collimated light beam certain distance L 3
F3, focal length f3) collimated light beam is focused again, it, can be in certain distance according to the focal length parameter of the lens of selection
Desired spot size is obtained at L4.By adjusting the distance between the first condenser lens 2131 and collimation lens 2132
It realizes in the continuously adjustable of fixed position laser facula size.
Fig. 6 is shown in the case where parameter is as shown in table 1, (the light that solid state laser 212 is emitted of different ω 0 that software simulates
Spot diameter size) under ω 1 (fixed position laser spot diameter size) and the first condenser lens 2131 displacement relationship
Curve.
Table 1
In a specific example, light path system 213 further includes optical filter (not shown).Optical filter can be but not limited to
Neutral-density filter etc., for adjusting the energy of laser beam.More specifically, optical filter can be set to the second condenser lens 2133 it
Afterwards, with the energy for adjusting the laser beam after being focused by the second condenser lens 2133.
Further, which further includes optical filtering regulating mechanism.Optical filtering regulating mechanism is connect with optical filter, with
In drive optical filter rotation.Optical filtering regulating mechanism can be the mechanisms such as steering engine, and rotation can drive optical filter to rotate, Jin Ershi
Now laser ability is continuously adjusted.
For the volume for reducing entire laser cell 210 as much as possible, the utility model carries out the structure of laser cell 210
Creative optimization design.In a specific example, shell 214 uses Modular Structure Design, including sequentially connected
First lens housing module 2141, the second lens housing module 2142 and the third lens housing module 2143.First condenser lens
2131, collimation lens 2132 and the second condenser lens 2133 are respectively arranged on the first lens housing module 2141, the second shells of lenses
In module 2142 and the third lens housing module 2143.
It can be movable relatively between first lens housing module 2141 and the second lens housing module 2142 poly- to adjust first
The distance between focus lens 2131 and collimation lens 2132 can such as fix the position of the first condenser lens 2131, and collimation is saturating
The position of mirror 2132 is adjustable；Or the position of the first condenser lens 2131 is adjustable, the position of collimation lens 2132 is fixed；Or
It is adjustable that first condenser lens 2131 and collimation lens 2132 are designed as position simultaneously.Preferably, which further includes
Lens adjusting mechanism.Lens adjusting mechanism is connect with the first lens housing module 2141 and/or the second lens housing module 2142,
With the relative position for adjusting the first lens housing module 2141 and the second lens housing module 2142.Lens adjusting mechanism can
It to be the stepper motor etc. of microminiature, can be automatically controled, degree of regulation is high, easy to operate.
Shell 214 is designed using modular structure, greatly reduces the difficulty of installation and debugging, and be easy to implement tune
The distance between the first condenser lens 2131 and collimation lens 2132 are saved, it such as can be by the first lens housing module 2141 and second
Lens housing module 2142 is threadedly coupled, and drives the first lens housing module 2141 and/or second saturating by lens adjusting mechanism
The rotation of mirror shell module 2142 carries out fine position, realizes the continuously adjustable of final spot size.
Further, such as in Fig. 3 and specific example shown in Fig. 4, shell 214 further includes the first reflection housing module
2144, the second reflection housing module 2145 and third reflect housing module 2146, and correspondingly, light path system 213 further includes being set to
The first reflecting mirror 2134 in first reflection housing module 2144, the second reflecting mirror in the second reflection housing module 2145
2145 and set on third reflection housing module 2146 in third reflecting mirror 2136.First reflection housing module 2144 is connected to the
Between one lens housing module 2141 and the second lens housing module 2142, the second reflection housing module 2145 is connected to second thoroughly
Between mirror shell module 2142 and the third lens housing module 2143, third reflection housing module 2146 is connected to the third lens shell
After module 2143.Pass through the first reflecting mirror 2134 of setting, the second reflecting mirror 2135 and third reflecting mirror 2136, Ke Yishi
Now the incident angle of laser beam is adjusted, and is advantageously implemented and the optimization of the length and volume of entire light path system 10 is set
Meter.
Further, in the specific example of diagram, the first reflection housing module 2144, second reflects housing module 2145
It is fixed by screws in optics fixed platform 211 with third reflection housing module 2146.First reflection housing module 2144, the
The both ends of two reflection housing modules 2145 and third reflection housing module 2146, which all have, sets internal thread outer cylinder, the first lens
Housing module 2141, the second lens housing module 2142 and the third lens housing module 2143 are with externally threaded cylindric knot
Structure reflects housing module 2145 with the first reflection housing module 2144, second respectively as inner cylinder and third reflects housing module
It is connected through a screw thread structure between 2146 threaded connections namely adjacent housing module to link together, the first lens housing mould
Relative motion is adjusted by spiral adjustment structure between block 2141 and the second lens housing module 2142.Shell 214
Each housing module be connected and fixed according to the mounting means of bushing type, install and debug and is very convenient.
In a specific example, laser cell 210 further includes Reflective regulation mechanism.Reflective regulation mechanism and the first reflection
Mirror 2134, the second reflecting mirror 2135 and/or the connection of third reflecting mirror 2136, can be light with the angle for adjusting corresponding reflecting mirror
Pine nut is existing to the adjusting in laser emitting direction and the adjusting of focus position.
Different housings module can be placed in different location according to demand by modular shell 214, flexible and changeable, this
Outside, it can also increase as needed for carrying out flat-top processing to laser facula, the laser of Gaussian Profile is enable to become flat-top energy
It measures the optical shaping eyeglass of equally distributed laser beam, and/or is touched for carrying out zero shake to mass spectrograph electric control pulse system
Photoelectricity trigger module of hair etc..
The shell 214 can also reflect housing module 2145 and third without containing the first reflection housing module 2144, second
Housing module 2146 is reflected, or reflects housing module 2145 and third reflection containing the first reflection housing module 2144, second
One or two of housing module 2146, and the first reflection housing module 2144, second reflects housing module 2145 and third
Reflecting mirror direction in reflection housing module 2146 also can according to need setting.Optical system 20 shown in Fig. 8 does not contain
Second reflection housing module, and the third reflecting mirror in third transmitting housing module 21 is arranged downward, and solid state laser 22 is sent out
Laser out is finally exported along the direction perpendicular to optics fixed platform 23.In addition, optical system 20 shown in Fig. 8 further includes light
Electric trigger module 24 and optical filter 25.
In the present embodiment, the laser that laser cell 210 issues is mapped to sample surfaces after the reflection of optical reflection mechanism 240
And the angle of the laser beam of reflection and the axis perpendicular to sample target plate 120 is greater than 0 ° and is not more than 10 ° of (laser lights of reflection
Angle formed by line and sample target plate 120 is less than 90 ° and is not less than 80 °), namely incident laser is with very small angle incidence
Onto sample target plate 120.Preferably, the laser beam of reflection and the angle of the axis perpendicular to sample target plate 120 are not more than 6 °,
It such as can be 3 °, 4 °, 5 ° or 6 °, further preferably 4 °~5 °.
Further, the illumination light that the lighting unit 220 of the present embodiment issues is irradiated to after the reflection of optical reflection mechanism 240
The angle of sample surfaces and illumination light light and the axis perpendicular to sample target plate 120 after the reflection of optical reflection mechanism 240
Greater than 0 ° and no more than 10 °, (the illumination light light and angle formed by sample target plate 120 reflected through optical reflection mechanism 240 is small
In 90 ° and it is not less than 80 °), namely incident illumination light is in very small angular illumination to sample target plate 120.Preferably, it passes through
Illumination light light and the angle of the axis perpendicular to sample target plate 120 after the reflection of optical reflection mechanism 240 are not more than 6 °, such as may be used
To be 3 °, 4 °, 5 ° or 6 ° etc., further preferably 4 °~5 °.
Imaging unit 230 is used to receive the illumination light reflected by sample.Sample reflection illumination light light with it is anti-through optics
The illumination light light of the reflection of mechanism 240 is penetrated to be symmetrical arranged perpendicular to the vertical line of sample target plate 120.In a specific example
In, the illumination light of sample reflection injects imaging unit 230 after reflecting via optical reflection mechanism 240.
More specifically, imaging unit 230 includes optical lens 232 and filming apparatus 234.It is anti-by optical reflection mechanism 240
Intake filming apparatus 234 is imaged after the illumination light of injection is captured via optical lens 232.Filming apparatus 234 can be CCD
Imaging device, and can also be connect with external connection display apparatus, carry out the display of shooting image.
By taking specific example shown in Fig. 2 as an example, laser cell 210, lighting unit 220 and imaging unit 230 surround from
The setting of component cavity 110.Laser cell 210, lighting unit 220 and imaging unit 230 is corresponded on ion source cavity 110 to open
If there are three transparent windows.
Preferably, the light source of lighting unit 220 and the optical lens 232 of imaging unit 230 are oppositely arranged.
It is highly preferred that the incident direction for the laser that laser cell 210 issues is perpendicular to after the reflection of optical reflection mechanism 240
Illumination light light with as sample reflect illumination light light determined by plane.
In a specific example, the tool of optical reflection mechanism 240 is respectively used to there are three reflecting surface 242 by incidence
Laser reflection reflexes to sample to sample, by the illumination light that lighting unit 220 issues and reflects the illumination light of sample reflection
To imaging unit 230.As shown in Fig. 2, with the illumination light light and vertical sample target plate 120 that are reflected through optical reflection mechanism 240
Axis in for 5 ° of angles, being in 42.5 ° of angle for the reflecting surface 242 of indirect illumination light and incident illumination light light,
Similarly, also it is in the illuminating ray for being emitted to imaging unit 230 by the reflecting surface 242 of the illumination light of sample reflection for reflecting
42.5 ° of angle.Equally, when the laser beam reflected through optical reflection mechanism 240 and the axis perpendicular to sample target plate 120
It is also in 42.5 ° of angle for the reflecting surface 242 of reflection laser and incident laser beam when angle is also 5 °.
Further, by taking top is positive the ion source cavity 110 of octahedral column structure as an example, it is preferable that lighting unit 220 with
Imaging unit 230 corresponds respectively to two opposite side walls of the octahedral cylinder structure division of ion source cavity 110, laser cell
210 correspond to another side wall of the octahedral cylinder structure division of ion source cavity 110 and the side wall and lighting unit 220 or imaging
One side wall is separated between the corresponding side wall of unit 230.Laser optical path is not shown in the figure in signal shown in Fig. 2, and laser optical path hangs down
Directly in the illumination light light after the reflection of optical reflection mechanism 240 and plane determined by the illumination light light as sample reflection.
Further, in a specific example, optical reflection mechanism 240 is located at the middle part of ion source cavity 110, through light
The illumination light light after reflecting mechanism 240 reflects is learned with the illumination light light by sample reflection with the axis of ion source cavity 110
Line is symmetrical.
In order to reduce the angle in laser light incident direction Yu mass spectrum tof tube axis as far as possible, entire ion source component 100
Pole piece component 130 etc. is all mounted on the lower section of optical reflection mechanism 240, and draws the design of the pole piece inner hole 134 of pole piece 132
It can guarantee that laser and illumination light can smoothly reach sample surfaces without the influence by pole piece component 130.Therefore, through anti-
Laser after penetrating can reach sample surfaces with very small angle, and by sample ionization, the area source for illuminating generation can lead to
The hole array crossed among pole piece component 130 reaches imaging unit 230.
Fig. 7 is to carry out actual facula measurement result to focus point using laser cell 210 shown in Fig. 3 and Fig. 4, the results showed that
The minimum focusing effect that can be achieved 16*14 μm of the laser cell 210.
Above-mentioned Mass Spectrometer Method device 10 can significantly improve mass spectral analysis with low-angle laser light incident, illumination and imaging
Resolution ratio and image quality.Specifically, the laser that laser cell 210 issues is mapped to sample after the reflection of optical reflection mechanism 240
Product surface and reflection laser beam with perpendicular to sample target plate 120 axis angle be greater than 0 ° and be not more than 10 °, in this way by
The grain that angle is very small, and laser ionization generates is formed by the incident direction of laser and the axis direction of ion source cavity 110
Son initial velocity direction be substantially along ion source cavity 110 central axis direction dissipate, therefore ion initial kinetic energy diverging compared with
It is small, be conducive to the resolution ratio and sensitivity that improve Mass Spectrometer Method device 10.And the illumination light that lighting unit 220 issues is anti-through optics
Penetrate mechanism 240 reflect after be irradiated to sample surfaces and through optical reflection mechanism 240 reflection after illumination light light with perpendicular to sample
The angle of the axis of product target plate 120 is greater than 0 ° and no more than 10 °, and imaging unit 230 is used to receive the illumination light reflected by sample,
The angle of imaging optical path is also very small in this way.And illumination and imaging direction are full symmetric, thus lighting source is in sample target plate 120
The crossover range of area that the irradiated area and imaging unit 230 that surface generates are imaged can achieve maximization, both can be to avoid
The generation of evil shadow effect, and the range of imaging can be greatly improved, and then image quality greatly improves.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But it cannot be understood as the limitations to utility model patent range.It should be pointed out that for the common skill of this field
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The protection scope of the utility model.Therefore, the scope of protection shall be subject to the appended claims for the utility model patent.
Claims (10)
1. a kind of optical system of Mass Spectrometer Method device, which is characterized in that including laser cell, lighting unit, imaging unit with
And optical reflection mechanism；The laser cell, the lighting unit and the imaging unit are set to the Mass Spectrometer Method device
Ion source cavity outside, the optical reflection mechanism be set to the ion source cavity inside；
The laser of the laser cell is solid state laser, and the laser that the laser cell issues is through the optical reflection mechanism
It is mapped to sample surfaces after reflection and the laser beam of reflection is greater than 0 ° with the angle of the axis perpendicular to sample target plate and is not more than
10°；The illumination light that the lighting unit issues is irradiated to sample surfaces after optical reflection mechanism reflection and through the light
The angle of illumination light light after learning reflecting mechanism reflection and the axis perpendicular to sample target plate is greater than 0 ° and is not more than 10 °；Institute
Imaging unit is stated for receiving the illumination light reflected by the sample to be imaged for sample.
2. optical system as described in claim 1, which is characterized in that the laser cell includes optics fixed platform and is set to
The solid state laser, light path system and shell in the optics fixed platform；
The light path system includes the first condenser lens, collimation lens and the second condenser lens, what the solid state laser issued
Laser beam generates the laser beam of diverging through first condenser lens, and the laser beam of the diverging becomes after the collimation lens
The laser beam of the laser beam of collimation, the collimation can be anti-through the optical reflection mechanism after second condenser lens focusing
It is incident on sample after penetrating；
First condenser lens, the collimation lens and second condenser lens are disposed in the housing, and described
The distance between one condenser lens and the collimation lens are adjustable.
3. optical system as claimed in claim 2, which is characterized in that the light path system further includes optical filter, the optical filtering
Piece is used to adjust the energy of laser beam.
4. optical system as claimed in claim 3, which is characterized in that the shell is modular construction, including is sequentially connected
The first lens housing module, the second lens housing module and the third lens housing module；First condenser lens, the standard
Straight lens and second condenser lens be respectively arranged on the first lens housing module, the second lens housing module and
In the third lens housing module；
It can be movable relatively between the first lens housing module and the second lens housing module poly- to adjust described first
The distance between focus lens and the collimation lens.
5. optical system as claimed in claim 4, which is characterized in that the shell further includes the first reflection housing module,
Two reflection housing modules and/or third reflect housing module, and correspondingly, the light path system further includes being set to first reflection
The first reflecting mirror in housing module, the second reflecting mirror in the second reflection housing module and/or it is set to described the
Third reflecting mirror in three reflection housing modules；
The first reflection housing module is connected between the first lens housing module and the second lens housing module,
The second reflection housing module is connected between the second lens housing module and the third lens housing module, described
Third reflection housing module is connected to after the third lens housing module.
6. optical system as claimed in claim 5, which is characterized in that be connected through a screw thread structure between adjacent housing module
It links together；
Relative motion is by spirally adjusting knot between the first lens housing module and the second lens housing module
What structure was adjusted.
7. optical system as claimed in claim 6, which is characterized in that the laser cell further include optical filtering regulating mechanism, thoroughly
At least one regulating mechanism in mirror adjustment mechanism and Reflective regulation mechanism；
The optical filtering regulating mechanism is connect with the optical filter, for driving the optical filter to rotate；
The lens adjusting mechanism is connect with the first lens housing module and/or the second lens housing module, with
In the relative position for adjusting the first lens housing module and the second lens housing module；
The Reflective regulation mechanism connect with first reflecting mirror, second reflecting mirror and/or the third reflecting mirror with
For adjusting the angle of corresponding reflecting mirror.
8. such as optical system according to any one of claims 1 to 7, which is characterized in that the optical reflection mechanism has three
A reflecting surface is respectively used to the incident laser reflection is anti-to the sample, the illumination light for issuing the lighting unit
It is incident upon the sample and the illumination light that the sample reflects is reflexed into the imaging unit.
9. such as optical system according to any one of claims 1 to 7, which is characterized in that the imaging unit includes optical frames
Head and filming apparatus, the illumination light reflected by the optical reflection mechanism take in the bat after capturing via the optical lens
Take the photograph device.
10. a kind of Mass Spectrometer Method device, which is characterized in that including ion source component and such as any one of claim 1~9 institute
The optical system stated；The ion source component includes ion source cavity, sample target plate and pole piece component, the sample target plate and
The pole piece component is set to the lower section in the ion source chamber body and being located at the optical reflection mechanism；The laser cell is located at
The side of the ion source cavity.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821568695.3U CN209069862U (en) | 2018-09-25 | 2018-09-25 | Mass Spectrometer Method device and its optical system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821568695.3U CN209069862U (en) | 2018-09-25 | 2018-09-25 | Mass Spectrometer Method device and its optical system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110942973A (en) * | 2018-09-25 | 2020-03-31 | 广州禾信康源医疗科技有限公司 | Mass spectrometer and optical system thereof |
| CN110940723A (en) * | 2018-09-25 | 2020-03-31 | 广州禾信康源医疗科技有限公司 | Mass spectrum detection device and optical system thereof |
-
2018
- 2018-09-25 CN CN201821568695.3U patent/CN209069862U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110942973A (en) * | 2018-09-25 | 2020-03-31 | 广州禾信康源医疗科技有限公司 | Mass spectrometer and optical system thereof |
| CN110940723A (en) * | 2018-09-25 | 2020-03-31 | 广州禾信康源医疗科技有限公司 | Mass spectrum detection device and optical system thereof |
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Address after: 510535 building 501, No. 1, No. 16, Xinrui Road, Huangpu District, Guangzhou, Guangdong Province (office only) Patentee after: GUANGZHOU HEXIN KANGYUAN MEDICAL TECHNOLOGY CO.,LTD. Address before: 510700 3 / F, building A3, science and technology enterprise accelerator, 11 Kaiyuan Avenue, Huangpu District, Guangzhou City, Guangdong Province Patentee before: GUANGZHOU HEXIN KANGYUAN MEDICAL TECHNOLOGY CO.,LTD. |
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