CN208980705U - The micro-fluidic chip of cell aggregation is carried out based on microelectrode array - Google Patents
The micro-fluidic chip of cell aggregation is carried out based on microelectrode array Download PDFInfo
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Abstract
The technical issues of a kind of micro-fluidic chip carrying out cell aggregation based on microelectrode array, is related to microfluidic art, and the solution is to high-flux cell collections.The chip includes the container floor stacked gradually from top to bottom, microelectrode layer, basal layer；The container floor is equipped at least one container hole and at least two electrode holes, is plugged with positive electricity pole pipe, negative electrode tube respectively in each electrode hole；The microelectrode layer is made of at least one planar electrode array, the microelectrode piece that each planar electrode array is tiled by even number forms, the hole heart that each microelectrode piece of each planar electrode array surrounds corresponding container hole is symmetrically laid, and each microelectrode piece is divided into positive microelectrode piece, negative microelectrode piece, each positive microelectrode piece is coupled with each positive electricity pole pipe, and each negative microelectrode piece is coupled with each negative electrode tube.Chip provided by the utility model may be implemented high-throughput various kinds of cell and collect.
Description
Technical field
The utility model relates to microflow control techniques, carry out the micro- of cell aggregation based on microelectrode array more particularly to a kind of
The technology of fluidic chip.
Background technique
Microfluidic chip technology is to be put forward for the first time by the Manz and Widmer of Switzerland early 1990s, due to its tool
Have analysis speed is fast, reagent consumption less, be easily integrated and many advantages, such as high throughput analysis, over the last couple of decades, miniflow
Control chip in terms of the analysis processing of biological and chemical sample achieves significant progress, in recent years microfluidic chip technology
Researcher has been transferred to research direction increasingly complex biosystem, such as carries out cell analysis using micro-fluidic chip
The research such micro-fluidic chip of plays more the exploratory development in the fields such as cell biology, neurology, pharmacy and organizational project
Carry out more important role.
Micro-fluidic chip cell operation is that cell is transported to scheduled site, after being fixed, carries out cell component, knot
The analysis such as structure and function.Existing microflow control technique all uses micro Process mode to make micro- well, micropore, Wei Ba, micro- slit and micro-pipe
The geometry trap such as road or obstacle capture cell, can not only form open array system, can also realize in microchannel to thin
The control of born of the same parents.But the processing request of micro Process mode is higher, and microchannel is easy blocking, preparation of samples requires high, discomfort
Close the collection of high-throughput various kinds of cell.
Utility model content
For above-mentioned defect existing in the prior art, the technical problem to be solved by the utility model is to provide one kind not
Easily blocking, it is low to sample requirement, and the micro-fluidic chip that cell aggregation is carried out based on microelectrode array of handling ease.
In order to solve the above-mentioned technical problem, provided by the utility model a kind of based on microelectrode array progress cell aggregation
Micro-fluidic chip, it is characterised in that: including container floor, the microelectrode layer, basal layer stacked gradually from top to bottom；
The container floor is equipped at least one container hole and at least two electrode holes, and the container hole, electrode hole are upward
It is open, a conductive electrode tube is plugged in each electrode hole；
Each electrode tube is divided into two groups, and one group of electrode tube therein is positive electrode tube, and another group of electrode tube is negative electrode tube；
The microelectrode layer is made of at least one planar electrode array, and each planar electrode array is tiled by even number
Microelectrode piece composition, each planar electrode array of microelectrode layer and each container hole of container floor correspond；
The hole heart that each microelectrode piece of each planar electrode array surrounds corresponding container hole is symmetrically laid, and each
Microelectrode piece is divided into two groups, and one group of microelectrode piece therein is positive microelectrode piece, and another group of microelectrode piece is negative microelectrode piece,
Each positive microelectrode piece is coupled with each positive electricity pole pipe, and each negative microelectrode piece is coupled with each negative electrode tube.
Further, each microelectrode piece of each planar electrode array has the lower end of regional area with corresponding container hole
Aperture overlapping.
Further, the microelectrode piece is gold electrode piece.
Further, the electrode tube is the copper pipe of surface gold-plating.
Further, the basal layer is slide of the thickness less than 2 millimeters.
The micro-fluidic chip provided by the utility model that cell aggregation is carried out based on microelectrode array, using flat geometry
Configuration makes microelectrode piece on the base layer, applies the control electricity that voltage signal is collected as dielectrophoresis in microelectrode on piece
Pressure, so that dielectrophoresis process be made to realize on each container hole, container hole, electrode hole aperture can design relatively
Greatly, therefore lower to the processing request of container hole, electrode hole, and container hole is also not easy to plug, the requirement to sample is also relatively
It is low.
Detailed description of the invention
Fig. 1 is the vertical view knot of the micro-fluidic chip that cell aggregation is carried out based on microelectrode array of the utility model embodiment
Structure schematic diagram；
Fig. 2 is that the left view of the micro-fluidic chip for carrying out cell aggregation based on microelectrode array of the utility model embodiment is cutd open
Cut figure.
Specific embodiment
The embodiments of the present invention are described in further detail below in conjunction with Detailed description of the invention, but the present embodiment and are not had to
In limitation the utility model, all similar structures and its similar variation using the utility model should all be included in the utility model
Protection scope, the pause mark in the utility model indicates the relationship of sum, and the English alphabet in the utility model is case sensitive.
As Figure 1-Figure 2, one kind provided by the utility model embodiment is based on microelectrode array and carries out cell aggregation
Micro-fluidic chip, it is characterised in that: including the container floor 1, microelectrode layer 2, basal layer 3 stacked gradually from top to bottom；
The container floor 1 is equipped with multiple containers hole 11 and multiple electrodes hole 12, and the container hole 11, electrode hole 12 are upwards
It is open, a conductive electrode tube 13 is plugged in each electrode hole 12；
Each electrode tube 13 is divided into two groups, and one group of electrode tube 13 therein is positive electrode tube, and another group of electrode tube 13 is negative
Electrode tube；
The microelectrode layer 2 is made of multiple planar electrode arrays, micro- electricity that each planar electrode array is tiled by four
Pole piece 21 forms, and each planar electrode array of microelectrode layer 2 and each container hole 11 of container floor 1 correspond；
The hole heart that four microelectrode pieces 21 of each planar electrode array surround corresponding container hole 11 is symmetrically laid, and
Each microelectrode piece 21 has that regional area is Chong Die with the lower end aperture of container hole 11, and four microelectrode pieces 21 are divided into two
Group, one group of microelectrode piece 21 therein are positive microelectrode piece, and another group of microelectrode piece 21 is negative microelectrode piece, each positive microelectrode
Piece 21 is coupled with each positive electricity pole pipe 13, and each negative microelectrode piece 21 is coupled with each negative electrode tube 13.
In the utility model embodiment, the container hole number on the container floor has 1 to 200, the preferred hole of container hole
Diameter is 4 millimeters, and there are four the microelectrode pieces in planar electrode array, the gold electrode piece of 100 nanometers of microelectrode piece preferred thickness, together
The laying spacing of each microelectrode piece in one planar electrode array is 100um；The preferred surface gold-plating of electrode tube in electrode hole
Copper pipe；In the utility model other embodiments, the quantity of the microelectrode piece in planar electrode array is also possible to other quantity, but
The quantity for being microelectrode piece should be even number (such as 2,6 etc.), can make the quantity one of positive and negative microelectrode piece in this way
It causes, electrode tube can also be using the pipe fitting, such as pure bobbin etc. that can be conductive of other materials.
In the utility model embodiment, the basal layer is the thickness by the preparation of glass, quartz or calcirm-fluoride less than 2 millimeters
Diascope, the microelectrode piece in planar electrode array on the base layer, then passes through bonding mode connection by coated film deposition
Container floor.
The voltage signal generator that the utility model embodiment cooperation can export 0.5V to 50V voltage uses, and may be implemented
High-throughput various kinds of cell is collected, stimulation of the parallel parsing different pharmaceutical concentration to different cells and its real-time sight in piece
It examines and detects, can be used for various concentration concurrent effect various kinds of cell, carry out the screening of high-flux cell drug susceptibility.
For collecting Escherichia coli, Escherichia coli solution is injected into each container hole, by the electricity of voltage signal generator
Pressure signal output end is connected to the electrode tube in each electrode hole, and voltage signal generator exports the voltage signal of an alternate peaks
(for example frequency is the 4V alternating current of 1MHz) is loaded into the electrode tube in each electrode hole, and then is loaded into planar electrode array
Each microelectrode piece, voltage signal driving under generate inhomogeneous field formed dielectrophoretic motion, resulting electrophoretic force
It is directed toward the center (and hole heart of container hole) of planar electrode array, it is after a period of time (such as 20 minutes), aobvious by fluorescence
Micro mirror can see the Escherichia coli being collected into and form a compact three-dimensional globoid.
Dielectrophoresis is the lower object of dielectric constant orientation of stress generation due to polarization in non-uniform electric field
Movement, can effectively realize accurate positioning, separation, collection, capture and transport of micron ion etc..Dielectric power size is with object
No electrification is unrelated, field strength, the field strength change with the size, electrical properties, the electrical properties of surrounding medium and extra electric field of object
Rate, frequency are related.
Claims (5)
1. a kind of micro-fluidic chip for carrying out cell aggregation based on microelectrode array, it is characterised in that: including from top to bottom successively
Container floor, the microelectrode layer, basal layer of stacking；
The container floor is equipped at least one container hole and at least two electrode holes, and the container hole, electrode hole open upwards,
A conductive electrode tube is plugged in each electrode hole；
Each electrode tube is divided into two groups, and one group of electrode tube therein is positive electrode tube, and another group of electrode tube is negative electrode tube；
The microelectrode layer is made of at least one planar electrode array, micro- electricity that each planar electrode array is tiled by even number
Pole piece composition, each planar electrode array of microelectrode layer and each container hole of container floor correspond；
The hole heart that each microelectrode piece of each planar electrode array surrounds corresponding container hole is symmetrically laid, and each micro- electricity
Pole piece is divided into two groups, and one group of microelectrode piece therein is positive microelectrode piece, and another group of microelectrode piece is negative microelectrode piece, each
Positive microelectrode piece is coupled with each positive electricity pole pipe, and each negative microelectrode piece is coupled with each negative electrode tube.
2. the micro-fluidic chip according to claim 1 for carrying out cell aggregation based on microelectrode array, it is characterised in that: every
Each microelectrode piece of a planar electrode array has regional area to be overlapped with the lower end aperture of corresponding container hole.
3. the micro-fluidic chip according to claim 1 for carrying out cell aggregation based on microelectrode array, it is characterised in that: institute
Stating microelectrode piece is gold electrode piece.
4. the micro-fluidic chip according to claim 1 for carrying out cell aggregation based on microelectrode array, it is characterised in that: institute
State the copper pipe that electrode tube is surface gold-plating.
5. the micro-fluidic chip according to claim 1 for carrying out cell aggregation based on microelectrode array, it is characterised in that: institute
Stating basal layer is slide of the thickness less than 2 millimeters.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110680527A (en) * | 2019-09-24 | 2020-01-14 | 西安交通大学 | Implant system, microelectrode module and dielectrophoresis method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110680527A (en) * | 2019-09-24 | 2020-01-14 | 西安交通大学 | Implant system, microelectrode module and dielectrophoresis method |
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