CN208872759U - A kind of vomitoxin detection card - Google Patents
A kind of vomitoxin detection card Download PDFInfo
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- CN208872759U CN208872759U CN201821658216.7U CN201821658216U CN208872759U CN 208872759 U CN208872759 U CN 208872759U CN 201821658216 U CN201821658216 U CN 201821658216U CN 208872759 U CN208872759 U CN 208872759U
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Abstract
The utility model discloses a kind of detections of vomitoxin to block, belong to vomitoxin Immunofluorescence test technical field, the detection card includes bracket and detection strip, being arranged side by side on the bracket has multiple box bodys, well and display port are offered on the box body, vertical columns on the bracket, the box body is fixed on the bracket by column, described box body one end offers opening, the detection strip is plugged in the box body by the opening, the box body is provided with lid in open at one end, magnetite pieces are fixed on the lid, iron plate is provided on the box body, the iron plate is connect with the magnetite pieces magnetism, it solves in detection multiple groups sample, by the testing result of multiple groups sample more cumbersome problem side by side.
Description
Technical field
The utility model relates to vomitoxin Immunofluorescence test technical field, in particular to a kind of vomitoxin detection
Card.
Background technique
Vomitoxin, also known as deoxynivalenol are a kind of trichothecenes, mainly by cereal sickle
The sickle-like bacteria such as knife bacterium, fusarium moniliforme, Fusarlum roseum, Fusarium nivale generate.Vomitoxin is fungi common in food
Toxin, pollution are widely present in world community, and toxigenic bacterium strain is suitable for growing under conditions of shady and cool, moist, mainly pollute small
The cereal crops such as wheat, barley, oat, corn, followed by oilseeds class raw material, also pollute cereal product, such as bread, biscuit, wheat system
Dessert etc..Vomitoxin has very strong toxicity to humans and animals, and humans and animals vomiting, diarrhea, food refusal, nerve can be caused disorderly
Unrest, miscarriage, stillborn foetus etc., pig are the animals most sensitive to vomitoxin.Vomitoxin chemical property is highly stable, generally will not be
It is destroyed in processing, storage and gastronomical process, 121 DEG C of hyperbaric heating 25min are only a small amount of to be destroyed, and acidic environment does not influence its poison
Power.
Currently used vomitoxin detection method has two class of confirmation method and rapid screening method, and confirmation method has high-efficient liquid phase color
Spectrometry, enzyme-linked immunosorbent assay etc..Such method high sensitivity, can quantitative detection, but need expensive instrument and skilled
Operator, detection time is long, and sample pre-treatments are also more complex, and especially each test experience personnel will contact the mark of severe toxicity
Quasi- product, so being not used to base or on-site supervision.The detection method time of applied immunology principle is short, flux is high, at low cost,
It is widely used.Immune colloidal gold technique is immune applied to one kind of antigen-antibody using colloidal gold as tracer label object
Labelling technique.Colloidal gold by gold chloride the reducing agent such as white phosphorus, ascorbic acid, sodium citrate, tannic acid the effects of under, polymerization becomes
The gold particle of particular size, and since electrostatic interaction becomes a kind of stable colloidal state.Colloidal gold can with conjugated protein and
Many other large biological molecules, the physical behavior having keep it wide such as high electron density, granular size, shape and color reaction
It is general to be applied to the fields such as immunology, histology, pathology and cell biology.Colloidal gold is at low cost, easy to use quick, is convenient for
Base use and scene use, but its can only qualitative detection, no standard measure.
The Chinese patent that Authorization Notice No. is CN206710440U, authorized announcement date is on December 05th, 2017 discloses one
The fluorescence immune chromatography kit of kind of rapid quantitative detection vomitoxin, including box body and lid, lid from box opening one
Side seal box body, lid are equipped with well and observation window, are equipped with immuno-chromatographic test paper strip, the immune chromatography test paper in box body
Item includes bottom plate, is overlapped with sample pad, bonding pad, reacting pad and suction according to by the square upstream sequence of one end to the other end on bottom plate
Water cushion.
It is in place of the deficiencies in the prior art, the corresponding immuno-chromatographic test paper strip of a tray interior, in detection multiple groups
Sample needs to be detected one by one using multiple fluorescence immune chromatography kits, if wanting to carry out the testing result of multiple groups sample
Compare side by side, then need to arrange in a line multiple fluorescence immune chromatography kits, operates more troublesome.
Utility model content
The purpose of the utility model is to provide a kind of vomitoxin detection block, which solve detection multiple groups sample in,
By the testing result of multiple groups sample more cumbersome problem side by side.
The above-mentioned technical purpose of the utility model has the technical scheme that
A kind of vomitoxin detection card, including bracket and detection strip, being arranged side by side on the bracket has multiple box bodys, institute
It states and offers well and display port on box body, vertical columns on the bracket, the box body is fixed on the branch by column
On frame, described box body one end offers opening, and the detection strip is plugged in the box body by the opening, the box body
Open at one end is provided with lid, magnetite pieces are fixed on the lid, iron plate, the iron plate and institute are provided on the box body
State the connection of magnetite pieces magnetism.
Using the above structure, a certain amount of detection liquid is taken to be added in well, so that detection liquid detects on detection strip.
When multiple groups sample detection, detection liquid can be added dropwise into different box body wells, different poison can also be inserted into respectively in multiple box bodys
The detection strip of element carries out detection comparison.After having detected, lid can be opened, take out the detection strip detected, change new
Detection strip can reuse.Scene uses or laboratory use is more convenient, convenient for being compareed from display port
Compare.
It advanced optimizes are as follows: support plate is fixed on the column, the box body is located in the support plate, adjacent described
It is fixed with positioning plate between column, magnet is fixed on the positioning plate, the box body is fixed in relatively described lid one end
There is iron plate, the iron plate is connect with the magnet magnetism.
Using the above structure, it convenient for removing box body from bracket, conveniently removes box body and is cleaned, sterilized or replaced
Deng use is more flexible.
It advanced optimizes are as follows: sliding rail is provided on the column, the box body two sides offer sliding slot, the sliding slot and institute
State sliding rail sliding setting.
Using the above structure, it is fixed convenient for positioning, improves the being completely embedded property and stability between box body and bracket.
Advanced optimize are as follows: the detection strip includes PVC board, be successively pasted in the PVC board sample pad, NC film and
Blotting paper, the sample pad side are bent upwards and are fixed on NC film upper surface, the blotting paper side be bent upwards and
It is fixed on NC film upper surface；
It is coated with the vomitoxin antibody of superconduction amount point label in the sample pad, has spraying vomitoxin on the NC film
The detection line of antigen and the control line for being coated with sheep anti mouse secondary antibody, the detection line are parallel to each other with the control line, the inspection
Survey line is close to the sample pad side, and the control line is close to the blotting paper side.
Using the above structure, a certain amount of detection liquid is taken to be added in well, detection liquid chromatographs in sample pad, is absorbing water
It is chromatographed under the action of paper to NC film direction, reaches detection line and control line, extra detection liquid are received by blotting paper, convenient for quick
Quantitative detection.
It advanced optimizes are as follows: the box body is there are three rectangle and settings, and four angles of three box bodys are all provided with
It is equipped with column.
It advanced optimizes are as follows: enable box body horizontal and be stably placed on bracket, to prevent influencing detection.
In conclusion detecting the utility model has the following beneficial effects: a certain amount of detection liquid is taken to be added in well
Liquid chromatographs in sample pad, chromatographs under the action of blotting paper to NC film direction, reaches detection line and control line, extra detection
Liquid is received by blotting paper.When multiple groups sample detection, detection liquid can be added dropwise into different box body wells, it can also be in three box bodys
The detection strip for being inserted into different toxin respectively carries out detection comparison.After having detected, lid can be opened, take out the detection detected
Strip, changing new detection strip can reuse.Scene uses or laboratory use is more convenient, is convenient for from display port
Observation, is compared.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of embodiment, is mainly used for embodying the structure of detection card；
Fig. 2 is the structural schematic diagram of embodiment, is mainly used for embodying the structure of detection card；
Fig. 3 is the partial structural diagram of embodiment, is mainly used for embodying the structure of detection card；
Fig. 4 is the structural schematic diagram of embodiment, is mainly used for embodying the structure of detection strip.
In figure, 1, bracket；2, strip is detected；21, PVC board；22, sample pad；23, NC film；24, blotting paper；25, it detects
Line；26, control line；3, box body；4, column；5, sliding slot；6, lid；7, magnetite pieces；8, iron plate；9, positioning plate；10, iron block；11,
Magnet；12, support plate；13, sliding rail；14, well；15, display port.
Specific embodiment
The utility model is described in further detail below in conjunction with attached drawing.
Embodiment: a kind of vomitoxin detection card, as shown in Figure 1,2,3, 4, including bracket 1 and detection strip 2.Bracket 1
Upper to be arranged side by side there are three rectangular box 3, four angles of three box bodys 3 are provided with column 4, and column 4 is fixed on bracket 1
On, box body 3 is fixed on bracket 1 by column 4.3 one end of box body offers opening, and detection strip 2 is plugged in box by opening
In body 3, box body 3 is provided with lid 6 in open at one end, and magnetite pieces 7 are fixed on lid 6, iron plate 8, iron plate are provided on box body 3
8 connect with 7 magnetism of magnetite pieces.Support plate 12 is fixed on column 4, box body 3 is located in support plate 12, solid between adjacent columns 4
Surely there is positioning plate 9, positioning plate 9 is located at 3 one end of box body, magnet 11 is fixed on positioning plate 9, box body 3 is in opposite 6 one end of lid
It is fixed with iron plate 8, iron plate 8 is connect with 11 magnetism of magnet.Sliding rail 13 is provided on column 4,3 two sides of box body offer sliding slot 5,
Sliding slot 5 and the sliding of sliding rail 13 are arranged.
Referring to Fig.1,2,3,4, detection strip 2 includes PVC board 21, is successively pasted with sample pad 22, NC film 23 in PVC board 21
With blotting paper 24,22 side of sample pad is bent upwards and is fixed on 23 upper surface of NC film, and 24 side of blotting paper is bent upwards and consolidates
It is scheduled on 23 upper surface of NC film.Sample pad 22 is made of glass fibre element film, and superconduction amount point label is coated in sample pad 22
Vomitoxin antibody.There is the detection line 25 of spraying vomitoxin antigen on NC film 23 and is coated with the control line of sheep anti mouse secondary antibody
26, detection line 25 is parallel to each other with control line 26, and detection line 25 is close to 22 side of sample pad, and control line 26 is close to blotting paper
24 sides.Well 14 and display port 15 are offered on box body 3, well 14 is corresponding with 22 position of sample pad, display port 15
It is corresponding with detection line 25 and 26 position of control line.
Use process and principle: taking a certain amount of detection liquid to be added in well 14, and detection liquid chromatographs in sample pad 22,
It is chromatographed under the action of blotting paper 24 to 23 direction of NC film, reaches detection line 25 and control line 26, extra detection liquid are absorbed water
Paper 24 receives.When multiple groups sample detection, detection liquid can be added dropwise into different 3 wells 14 of box body, can also divide in three box bodys 3
The detection strip 2 for not being inserted into different toxin carries out detection comparison.After having detected, lid 6 can be opened, take out the detection detected
Strip 2, changing new detection strip 2 can reuse.
For the detection performance for verifying the utility model, the vomitoxin rapid quantitative detection card provided in embodiment is carried out
Experiment detection: adding vomitoxin concentration respectively in being measured as 0 variety classes blank sample with National Standard Method is 200,500,
The standard items of 1000,1500,2500,5000 μ g/kg, it is then quickly fixed using vomitoxin provided by the utility model (DON)
Amount detection card is detected.Testing result is shown in Table 1.
The accuracy and repeatability of the different sample detections of table 1
As it can be seen from table 1 vomitoxin detection card provided by the utility model detects and vomits poison in different grain samples
The TIANZHU XINGNAO Capsul of element is higher, and the coefficient of variation is lower.
This specific embodiment is only the explanation to the utility model, is not limitations of the present invention, ability
Field technique personnel can according to need the modification that not creative contribution is made to the present embodiment after reading this specification, but
As long as all by the protection of Patent Law in the scope of the claims of the utility model.
Claims (5)
1. a kind of vomitoxin detection card, it is characterized in that: including bracket (1) and detection strip (2), on the bracket (1) side by side
It is provided with multiple box bodys (3), well (14) and display port (15) is offered on the box body (3), is fixed on the bracket (1)
Column (4), the box body (3) are fixed on the bracket (1) by column (4), and described box body (3) one end offers opening,
The detection strip (2) is plugged in the box body (3) by the opening, and the box body (3) is provided with lid in open at one end
Body (6) is fixed with magnetite pieces (7) on the lid (6), is provided with iron plate (8) on the box body (3), the iron plate (8) and institute
State the magnetic connection of magnetite pieces (7).
2. a kind of vomitoxin detection card according to claim 1, it is characterized in that: being fixed with support on the column (4)
Plate (12), the box body (3) are located in the support plate (12), are fixed with positioning plate (9), institute between the adjacent column (4)
It states and is fixed on positioning plate (9) magnet (11), the box body (3) is fixed with iron plate (8) in relatively described lid (6) one end,
The iron plate (8) connect with the magnet (11) magnetism.
3. a kind of vomitoxin detection card according to claim 1, it is characterized in that: being provided with sliding rail on the column (4)
(13), box body (3) two sides offer sliding slot (5), and the sliding slot (5) and the sliding rail (13) sliding are arranged.
4. a kind of vomitoxin detection card according to claim 1, it is characterized in that: the detection strip (2) includes PVC board
(21), sample pad (22), NC film (23) and blotting paper (24), the sample pad (22) are successively pasted on the PVC board (21)
Side is bent upwards and is fixed on NC film (23) upper surface, and blotting paper (24) side is bent upwards and is fixed on described
NC film (23) upper surface；
It is coated with the vomitoxin antibody of superconduction amount point label on the sample pad (22), has spraying to vomit on the NC film (23)
The detection line (25) of Venom antigens and the control line (26) for being coated with sheep anti mouse secondary antibody, the detection line (25) and the control line
(26) it is parallel to each other, the detection line (25) is close to the sample pad (22) side, and the control line (26) is close to described
Blotting paper (24) side.
5. a kind of vomitoxin detection card according to claim 1, it is characterized in that: the box body (3) is rectangle and setting
There are three, four angles of three box bodys (3) are provided with column (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821658216.7U CN208872759U (en) | 2018-10-12 | 2018-10-12 | A kind of vomitoxin detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821658216.7U CN208872759U (en) | 2018-10-12 | 2018-10-12 | A kind of vomitoxin detection card |
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| Publication Number | Publication Date |
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| CN208872759U true CN208872759U (en) | 2019-05-17 |
Family
ID=66470027
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201821658216.7U Active CN208872759U (en) | 2018-10-12 | 2018-10-12 | A kind of vomitoxin detection card |
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| Country | Link |
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| CN (1) | CN208872759U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113884631A (en) * | 2021-08-26 | 2022-01-04 | 中农康正技术服务有限公司 | Detection apparatus based on vomitoxin in agricultural product grains |
| CN115932249A (en) * | 2022-12-30 | 2023-04-07 | 杭州纽太生物科技有限公司 | Real-time calibration immunochromatography detection method |
-
2018
- 2018-10-12 CN CN201821658216.7U patent/CN208872759U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113884631A (en) * | 2021-08-26 | 2022-01-04 | 中农康正技术服务有限公司 | Detection apparatus based on vomitoxin in agricultural product grains |
| CN113884631B (en) * | 2021-08-26 | 2024-04-19 | 中农康正技术服务有限公司 | Detection device based on vomitoxin in agricultural product cereal |
| CN115932249A (en) * | 2022-12-30 | 2023-04-07 | 杭州纽太生物科技有限公司 | Real-time calibration immunochromatography detection method |
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