CN208549780U - Cell cryopreservation kit - Google Patents
Cell cryopreservation kit Download PDFInfo
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- CN208549780U CN208549780U CN201820743101.1U CN201820743101U CN208549780U CN 208549780 U CN208549780 U CN 208549780U CN 201820743101 U CN201820743101 U CN 201820743101U CN 208549780 U CN208549780 U CN 208549780U
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- reagent
- cell cryopreservation
- cell
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- fixed plate
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Abstract
The utility model relates to from the cell cryopreservation kit newly developed, the kit is sealing, including box body and openable box cover；The tray interior is equipped with fixed plate, several holes for being placed with reagent consumptive material and groove are set in the fixed plate, the reagent consumptive material includes reagent bottle, pipette, mixing vessel, cryopreservation tube/freeze bag, mentioned reagent consumptive material is sealing, sterile, for reagent bottle dress there are three types of frozen stock solution reagent, mixing vessel, cryopreservation tube/freeze bag are old soldi.This kit is easy to use, is suitable for freeze-stored cell, conducive to intracellular activity is maintained, cell recovery rate can be improved to avoid cross contamination, ensure that cell growth quality, shorten the proliferating cycle of cell recovery.
Description
Technical field
The utility model relates to kit or reagent box, in particular to a kind of cell cryopreservation kit.
Background technique
Cryo-conservation technology is a kind of method that the long-time being widely used saves biological sample.Usually by biology living
Body is cooled to low temperature (generally -196 DEG C) using special method, and long-term preservation.It when needed, can be by organism according to spy
Different method is heated to normal temperature, still can get the organism of survival.This method is storage cell and preservation cell characteristics
Effective ways.
Cell freezing preservation needs that cell cools down first, and with the decline of temperature, intraor extracellular can freeze, and be formed by
Ice crystal will cause cell membrane and organelle damage.The factor for influencing degree of injury mainly has chilling rate, thawing speed and freezing
Protective agent.
First two factor chilling rate and thawing speed are limited by state-of-the-art, can not there is larger change in a short time
Under the premise of, the correct selection and application of cryoprotector just become determine to freeze effect and cell recovery rate, it is active most
Key factor.It is protectant simultaneously in research novel freezing, it is also desirable to further to develop the novel suitable freeze-stored cell of one kind
Kit directly freeze-stored cell and can guarantee the recovery vigor of cell.
Utility model content
It is easy to use the purpose of this utility model is to provide a kind of novel kit, it is suitable for cell cryopreservation, is conducive to
The activity of cell is maintained, and guarantees to turn out applicable cell from the cell of recovery.
Cell cryopreservation kit provided by the utility model is sealing, including box body and openable box cover, the box
Internal portion is equipped with fixed plate, and several holes for placing reagent consumptive material or groove are arranged in the fixed plate.Reagent consumptive material includes reagent
Bottle, pipette, mixing vessel, cryopreservation tube/freeze bag.
The reagent bottle is three and seals, is sterile, is respectively provided with three kinds of frozen stock solution reagents.
The pipette is to seal, is sterile, and number is 6-8 branch.
The mixing vessel is two sealings, sterile, empty reagent bottles.
The cryopreservation tube/freeze bag be it is sterile, number is 10-20/1-2.
The reagent bottle is flat aseptic bottle；Mixing vessel is the aseptic bottle of conical bottom；Cryopreservation tube is flat nothing
Bacterium bottle.
That the box body and the box cover are connected to connect or mutually independent, the box cover covers on the box body, makes institute
It states kit and keeps sealing state.
The fixed plate is fixed in the box body or the fixed plate is dismountable.
The reagent consumptive material is individually positioned in the hole or groove, plays fixed function.
The material of two reagent bottles and mixing vessel is transparent plastic, and the bottle material of another reagent is brown modeling
Material；All reagent consumptive materials are individually positioned in the hole or groove.
The material of the cell cryopreservation kit is polypropylene plastics material.
The cell cryopreservation kit, bottleneck are narrow-mouthed bottle, design inside bottleneck for frosted, keep sealing, prevent from trying
Agent leakage.
The utensil further includes refrigerating object, is placed in the groove.
The outside of the box cover is additionally provided with a handle.
The fixed plate is sponge fixed plate or foam fixing plate, is detachably mounted to tray interior.
The kit of the utility model, it is easy to use, it is suitable for freeze-stored cell, conducive to the activity for maintaining cell, can keeps away
Exempt from cross contamination, cell recovery rate can be improved, ensure that cell growth quality, shortens the proliferating cycle of cell recovery.
Kit of the invention can reach higher anabiosis rate and motility rate for freeze-stored cell.
Detailed description of the invention
Fig. 1 is the surface structure schematic diagram of the kit of the utility model.
Fig. 2 is the tray interior structural schematic diagram of the kit of the utility model.
1- box body；2- box cover；3- handle；4- fixed plate；5,6,7- reagent bottle；The hole 8- or groove；9- pipette；10- freezes
Deposit pipe/freeze bag；11,12- mixing vessel.
Specific embodiment
Present inventor after extensive and in-depth study, designs a kind of kit suitable for cell cryopreservation,
The kit is sealing, including box body and openable box cover, and the tray interior is equipped with fixed plate, sets in the fixed plate
Set several holes for being placed with reagent consumptive material or groove.Reagent consumptive material is to seal, is sterile.The kit freezes suitable for cell
It deposits, can directly use, it is convenient and simple, it conducive to the activity for maintaining cell, avoids pollution, keeps sterile.On this basis, it completes
The utility model.
Term
As used herein, " kit " is alternatively referred to as reagent box, has the same meaning, and is used to contain suitable for freezing
The reagent bottle of cell.
As used herein, the preservation liquid that " frozen stock solution " refers to dedicated for freeze-stored cell.Such as freezing immunocyte,
The immunocyte of extraction is put into the cryopreservation tube equipped with frozen stock solution, is frozen using conventional method and apparatus.Frozen stock solution
Use, it is ensured that the cell frozen is still active.
The present invention will be further illustrated below in conjunction with specific embodiments.It should be understood that these embodiments are merely to illustrate this
It utility model rather than limits the scope of the invention.In the following examples, the experimental methods for specific conditions are not specified, usually presses
More solito condition or according to the normal condition proposed by manufacturer.
Unless otherwise defined, it anticipates known to all professional and scientific terms as used herein and one skilled in the art
Justice is identical.In addition, any method similar to or equal to what is recorded and material all can be applied in the present invention.It is described in text
Preferred implement methods and materials be for illustrative purposes only.
As shown in Figure 1, the kit of the utility model is sealing, including box body (1) and openable box cover (2).
That the box body (1) and the box cover (2) are connected to connect or mutually independent, the box cover (2) is covered in the box
On body (1), the kit is made to keep sealing state.
For convenience of carrying, the outside of the box cover (2) is equipped with handle (3).
As shown in Fig. 2, fixed plate (4) are housed inside box body described in the utility model kit (1), the fixed plate (4)
On be provided with it is several place reagent consumptive materials holes or groove (8), the reagent consumptive material include reagent bottle (5), (6), (7), liquid relief
Pipe (9), mixing vessel (11), (12), cryopreservation tube/freeze bag (10).
The reagent bottle (5), (6), (7) are three and seal, is sterile, are respectively provided with three kinds of frozen stock solution reagents.
The pipette (9) is to seal, is sterile, and number is 6-8 branch.
The cryopreservation tube/freeze bag is that (10) are sterile, and number is 10-20/1-2.
The mixing vessel (11,12) is two sealings, sterile, empty reagent bottles.
The reagent bottle (5), (6), (7) are flat aseptic bottles；Mixing vessel (11,12) is the sterile of conical bottom
Bottle；Cryopreservation tube (10) is flat aseptic bottle.
That the box body (1) and the box cover (2) are connected to connect or mutually independent, the box cover (2) is covered in the box
On body (1), the kit is made to keep sealing state.
The fixed plate (4) is fixed in the box body (1) or the fixed plate is dismountable.
Two reagent bottles (5) of the cell cryopreservation kit, (6) and mixing vessel (11), the material of (12) are
Transparent plastic, bottle (7) material of another reagent are brown plastics；All reagent consumptive materials are individually positioned in the hole or groove
(8) in.
The cell cryopreservation kit, material are polypropylene plastics material.
The cell cryopreservation kit, bottleneck are narrow-mouthed bottle, design inside bottleneck for frosted, keep sealing, prevent from trying
Agent leakage.
The cell cryopreservation kit, refrigerating object are placed on the lower section of the fixed plate.
The fixed plate (4) is sponge fixed plate or foam fixing plate, and it is internal to be detachably mounted to box body (1).
Utility model has the advantages that
Kit is suitable for freeze-stored cell, can directly use, convenient and simple.
Conducive to the activity for maintaining cell, cell recovery rate can be improved to avoid cross contamination, ensure that cell grows matter
Amount, shortens the proliferating cycle of cell recovery.
Kit defines the proportion of cell and frozen stock solution, conducive to the cryopreservation resuscitation of cell.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail within the scope of the technical concept of the present invention can be with various simple variants of the technical solution of the present invention are made, this
A little simple variants all belong to the scope of protection of the present invention.
Claims (14)
1. a kind of cell cryopreservation kit, which is characterized in that the cell cryopreservation kit be sealing, including box body (1) and
Openable box cover (2), the box body (1) is internal to be equipped with fixed plate, and several reagents that are placed with are arranged in the fixed plate and consume
The hole of material or groove (8), reagent consumptive material include reagent bottle (5,6,7), pipette (9), cryopreservation tube or freeze bag (10), mixing appearance
Device (11,12).
2. cell cryopreservation kit according to claim 1, which is characterized in that the reagent bottle (5,6,7) is three
It is sealing, sterile, it is respectively provided with three kinds of frozen stock solution reagents.
3. cell cryopreservation kit according to claim 1, which is characterized in that the pipette (9) is sealing, nothing
Bacterium, number is 6-8 branch.
4. cell cryopreservation kit according to claim 1, which is characterized in that the cryopreservation tube or to freeze bag be (10)
Sterile, number is 10-20/1-2.
5. cell cryopreservation kit according to claim 1, which is characterized in that the mixing vessel (11,12) is two
A sealing, sterile, empty reagent bottle.
6. cell cryopreservation kit according to claim 1, which is characterized in that the reagent bottle (5,6,7) is flat
Aseptic bottle；Mixing vessel (11,12) is the aseptic bottle of conical bottom；Cryopreservation tube or to freeze bag (10) be flat aseptic bottle.
7. cell cryopreservation kit according to claim 1, which is characterized in that the box body (1) and the box cover (2) are
Being connected or mutually independent, the box cover (2) is covered on the box body (1), and the kit is made to keep sealing state.
8. cell cryopreservation kit according to claim 1, which is characterized in that the fixed plate (4) is fixed on the box
In body (1) or the fixed plate is dismountable.
9. cell cryopreservation kit according to claim 2, which is characterized in that two reagent bottles (5,6) and mixing vessel
The material of (11,12) is transparent plastic, and bottle (7) material of another reagent is brown plastics；All reagent consumptive materials are placed respectively
In the hole or groove (8).
10. cell cryopreservation kit according to any one of claim 1-3, which is characterized in that its material is poly- third
Alkene plastics material.
11. cell cryopreservation kit according to any one of claim 1-3, which is characterized in that bottleneck is narrow-mouthed bottle, bottle
Mouth is internal to be designed for frosted, is kept sealing, is prevented reagent from leaking outside.
12. cell cryopreservation kit according to claim 1, which is characterized in that refrigerating object is placed on the fixed plate
Lower section.
13. cell cryopreservation kit according to claim 1, which is characterized in that the outside of the box cover (2) is additionally provided with one
A handle (3).
14. cell cryopreservation kit according to claim 1, which is characterized in that the fixed plate (4) is sponge fixed plate
Or foam fixing plate, it is internal to be detachably mounted to box body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820743101.1U CN208549780U (en) | 2018-05-18 | 2018-05-18 | Cell cryopreservation kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820743101.1U CN208549780U (en) | 2018-05-18 | 2018-05-18 | Cell cryopreservation kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208549780U true CN208549780U (en) | 2019-03-01 |
Family
ID=65483491
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820743101.1U Active CN208549780U (en) | 2018-05-18 | 2018-05-18 | Cell cryopreservation kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208549780U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109997841A (en) * | 2019-04-09 | 2019-07-12 | 上海交通大学 | Maintain mankind's gamete fast freeze-thaw visible environment cabin of atmospheric air atmosphere |
-
2018
- 2018-05-18 CN CN201820743101.1U patent/CN208549780U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109997841A (en) * | 2019-04-09 | 2019-07-12 | 上海交通大学 | Maintain mankind's gamete fast freeze-thaw visible environment cabin of atmospheric air atmosphere |
| CN109997841B (en) * | 2019-04-09 | 2021-02-02 | 上海交通大学 | Human gamete rapid freezing and thawing visualization environment cabin maintaining atmospheric air atmosphere |
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