CN208420936U - A kind of carbendazim rapid detection card - Google Patents
A kind of carbendazim rapid detection card Download PDFInfo
- Publication number
- CN208420936U CN208420936U CN201821296950.3U CN201821296950U CN208420936U CN 208420936 U CN208420936 U CN 208420936U CN 201821296950 U CN201821296950 U CN 201821296950U CN 208420936 U CN208420936 U CN 208420936U
- Authority
- CN
- China
- Prior art keywords
- carbendazim
- pad
- gold
- antibody
- card body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model discloses a kind of carbendazim rapid detection cards, it is related to detecting card technique field, the utility model includes card body, sample pad is successively pasted in card body internal backplane, colloidal gold pad, coated film, and water absorption pad, card body is equipped with well and form hole, it further include the liquid feeding device to be checked with card body cooperation, liquid feeding device to be checked includes plate and round tube, round tube runs through plate center, it is sealed with plastic foil round tube bottom, the plastic film is covered with colloid gold label object, the cylindricality block across well is fixed in sample pad, in cone at the top of cylindricality block, the colloidal gold pad is resisting carbendazim Antibody Gold gold pad, the coated film is equipped with the detection line of the conjugate of antigen protein containing carbendazim and the nature controlling line of an antibody containing anti-rabbit or anti-mouse antibody；The utility model has easy to operate, the high advantage of detection efficiency by reducing dropper access times.
Description
Technical field
The utility model relates to detect card technique field, more particularly to a kind of carbendazim rapid detection card.
Background technique
China is production and the big country using chemical pesticide, and pesticide is used for a long time to the danger of ecological environment and human health
Evil and influence have caused the highest attention of people.Carbendazim [carbendazim, N- (2- benzopyrene oxazolyl) carbamic acid first
Ester] it is benzopyrene azole, it is a kind of good wide spectrum, systemic fungicide, in sac fungus, basidiomycetes and Fungi Imperfecti
Most of pathogens are all effective, are widely used in the disease control work of crops and Chinese herbal medicine.Carbendazim chemical property
Stablize, can be absorbed by the seed, root and leaf of plant, the residual period is longer, there is certain toxicity to people, animal, can cause twitch, spirit
Dimly, the poisoning symptoms such as nausea and vomiting, uncomfortable in chest, dizzy.Since carbendazim has widely in terms of the prevention and treatment of corps diseases
Using, but it has certain toxic to human body again, therefore the analysis in relation to Determination of carbendazim residue is increasingly taken seriously.
It is directed to carbendazim detection mode at present, the related detecting methods such as existing national standard are that gas phase, liquid phase or makings are used in conjunction
Method, such method equipment procurement is at high cost, and personnel requirement is high, reagent consumptive material is expensive, detection cycle is long, is unfavorable for base
Measuring station carry out primary dcreening operation residual to agriculture；Also there is the principle for taking immunology competition law, association colloid gold (or latex particle) marks skill
A kind of quick detection reagent of art and immunochromatographic method design, this method are needed when in use by measuring samples dilution successively
By colloid gold label and detection card two steps of detection, i.e., first with dropper by measuring samples dilution gold mark micropore, with mating drop
The soft piping and druming of pipe 30 seconds is completely dissolved gold mark micropore bottom red material, and horizontal rest waits reaction 2 minutes, and it is micro- to draw gold mark
Solution in hole is all added drop-wise in detection card well, in this process, since sample-adding amount is few, adds after soft piping and druming 10 second
The bad control of sample amount takes along with needing repeatedly to drip by dropper, so that entire detection process is inconvenient, it is cumbersome.
Utility model content
The purpose of this utility model is that: cumbersome in order to solve existing carbendazim detection card detection process, operating efficiency is low
The problem of, the utility model provides a kind of carbendazim rapid detection card.
The utility model specifically uses following technical scheme to achieve the goals above:
A kind of carbendazim rapid detection card, including card body, be from left to right successively pasted in card body internal backplane sample pad,
Colloidal gold pad, coated film and water absorption pad, card body are equipped with well and form hole, further include adding with the liquid to be checked of card body cooperation
Expect that device, liquid feeding device to be checked include the plate and round tube being fixedly connected, round tube runs through plate center, round tube bottom plastics
Film sealing, the plastic film are covered with the marker of antibody colloidal gold containing carbendazim, are fixed in sample pad across well
Cylindricality block, in cone at the top of cylindricality block, the colloidal gold pad is resisting carbendazim Antibody Gold gold pad, is set on the coated film
There are the detection line of the conjugate of antigen protein containing carbendazim and the nature controlling line of an antibody containing anti-rabbit or anti-mouse antibody.
Further, the coated film is nitrocellulose filter.
Further, the nitrocellulose filter both ends are mutually handed over resisting carbendazim Antibody Gold gold pad and water absorption pad respectively
Folded connection, the sample pad overlay in resisting carbendazim Antibody Gold gold pad.
Further, the round tube diameter is less than sample-adding bore dia.
Further, the card body is equipped with multiple positioning pins, and plate is equipped with the location hole with detent fit.
The beneficial effects of the utility model are as follows:
1. the detection card of the utility model has the advantages that detection is fireballing, while by the way that liquid feeding device to be checked is arranged,
And sealed in the round tube bottom of liquid feeding device to be checked with plastic foil, traditional gold mark micropore is replaced with round tube and plastic foil, to
Examine liquid in liquid feeding device to be checked with colloid gold label object ining conjunction with after, directly using liquid feeding device to be checked to liquid to be checked progress
Transfer, by feeding device plastic foil directly through cylindricality block, liquid to be checked directly enters well along cylindricality block and reaches sample pad,
Dropper access times are reduced, while well is added conveniently in liquid to be checked, reduces operation difficulty.
2. the utility model is less than sample-adding bore dia by setting round tube diameter, facilitates liquid to be checked that well is added, avoid
The unrestrained waste of liquid to be checked.
3. the utility model is positioned by the cooperation of setting positioning pin and hole convenient for feeding device, operation difficulty is reduced.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model
Fig. 2 is the structural schematic diagram of card body；
Fig. 3 is the structural schematic diagram of liquid feeding device to be checked；
Fig. 4 be cylindricality block with sample pad structural schematic diagram；
Appended drawing reference: 1- card body, 2- well, 3- form hole, 4- water absorption pad, 5- nature controlling line, 6- detection line, 7- coating
Film, 8- colloidal gold pad, 9- sample pad, 10- plate, 11- round tube, 12- plastic foil, 13- colloid gold label object, 14- positioning pin,
15- location hole.
Specific embodiment
It is practical new below in conjunction with this to keep the objectives, technical solutions, and advantages of the embodiments of the present invention clearer
Attached drawing in type embodiment, the technical scheme in the utility model embodiment is clearly and completely described, it is clear that is retouched
The embodiment stated is the utility model a part of the embodiment, instead of all the embodiments.Usually here in attached drawing description and
The component of the utility model embodiment shown can be arranged and be designed with a variety of different configurations.
Therefore, requirement is not intended to limit to the detailed description of the embodiments of the present invention provided in the accompanying drawings below
The scope of the utility model of protection, but it is merely representative of the selected embodiment of the utility model.Based in the utility model
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the range of the utility model protection.
It should also be noted that similar label and letter indicate similar terms in following attached drawing, therefore, once a certain Xiang Yi
It is defined in a attached drawing, does not then need that it is further defined and explained in subsequent attached drawing.In addition, term " the
One ", " second " etc. is only used for distinguishing description, is not understood to indicate or imply relative importance.
In the description of the utility model embodiment, it should be noted that the instructions such as term "inner", "outside", "upper"
Orientation or positional relationship is to be based on the orientation or positional relationship shown in the drawings or when utility model product use usually puts
The orientation or positional relationship put, is merely for convenience of describing the present invention and simplifying the description, rather than indication or suggestion is signified
Device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as to this is practical
Novel limitation.
Embodiment 1
As shown in figures 1 and 3, the present embodiment provides a kind of carbendazim rapid detection card, including card body 1,1 inner bottoms of card body
Sample pad 9, colloidal gold pad 8, coated film 7 and water absorption pad 4 are from left to right successively pasted on plate, card body 1 is equipped with well 2
It further include the liquid feeding device to be checked cooperated with card body 1, liquid feeding device to be checked includes the plate being fixedly connected with form hole 3
10 and round tube 11, round tube 11 runs through 10 center of plate, and 11 bottom of round tube is sealed with plastic foil 12,12 surface of the plastic foil covering
There is the marker of antibody colloidal gold containing carbendazim 13, the cylindricality block across well 2 is fixed in sample pad, is in circle at the top of cylindricality block
Taper, the colloidal gold pad 8 are resisting carbendazim Antibody Gold gold pad 8, and the coated film 7 is equipped with an egg of antigen containing carbendazim
The nature controlling line 5 of the detection line 6 of white matter conjugate and an antibody containing anti-rabbit.
Specifically, the coated film 7 is nitrocellulose filter.
Specifically, the nitrocellulose filter both ends are mutually handed over resisting carbendazim Antibody Gold gold pad 8 and water absorption pad 4 respectively
Folded connection, the sample pad 9 overlay in resisting carbendazim Antibody Gold gold pad 8.
Specifically, 11 diameter of round tube is less than 2 diameter of well, facilitates liquid to be checked that well 2 is added, avoids liquid to be checked
Unrestrained waste.
Working principle: traditional gold mark micropore is replaced with round tube 11 and plastic foil 12, liquid to be checked is in liquid feeding device to be checked
In combined with the marker of antibody colloidal gold containing carbendazim 13 after, directly liquid to be checked is shifted using liquid feeding device to be checked,
By liquid feeding device plastic foil 12 to be checked directly through cylindricality block, liquid to be checked directly enters well 2 along cylindricality block and reaches sample
Product pad 9 reduces dropper access times, while 2 convenience of well is added in liquid to be checked, reduces operation difficulty.
Embodiment 2
As shown in Fig. 2, the present embodiment advanced optimizes on the basis of embodiment 1, the specially described card body 1 is equipped with more
A positioning pin, plate 10 are equipped with the location hole 15 cooperated with positioning pin 14.
Application method: obtaining liquid to be checked after sample liquid is diluted, liquid feeding device to be checked is added in liquid to be checked with dropper, together
When with mating dropper softly blow and beat liquid to be checked 30 seconds, the red material being completely dissolved on plastic foil 12, horizontal rest waits reaction
2 minutes, liquid feeding device to be checked is moved into 1 well of card body, 2 top, positioning pin 14 is inserted into location hole 15, is gradually moved down
Feeding device pokes plastic foil 12 using cylindricality block, and liquid to be checked reaches sample pad 9 by well 2, waits 5 minutes, in form
Read reaction result in hole 3.
Negative: the colour developing of detection line 6 is deeper or equally deep than nature controlling line 5, shows that carbendazim pesticide residue concentration is lower than in sample
Detect Limited Doses or without carbendazim pesticide residue.
Positive: the colour developing of detection line 6 is more shallow than nature controlling line 5, shows that carbendazim pesticide residue concentration is higher than detection limitation in sample
Value；Detection line 6 is more shallow than nature controlling line 5, shows that carbendazim pesticide residue concentration is higher in sample.
Invalid: nature controlling line 5 does not occur aubergine band, shows that incorrect operating process or detection block damage of having gone bad
Carbendazim antigen protein conjugate, anti-rabbit antibody and Antibody Gold containing carbendazim used in the utility model
Golden marker is that market is bought, and details are not described herein again for preparation method.
Claims (5)
1. a kind of carbendazim rapid detection card, including card body (1), sample is from left to right successively pasted in card body (1) internal backplane
Product pad (9), colloidal gold pad (8), coated film (7) and water absorption pad (4), card body (1) are equipped with the well being connected to cavity inside
(2) and form hole (3), it is characterised in that: further include the liquid feeding device to be checked with card body (1) cooperation, liquid feeding device to be checked
Including the plate (10) and round tube (11) being fixedly connected, round tube (11) runs through plate (10) center, round tube (11) bottom plastics
Film (12) sealing, plastic foil (12) surface are covered with the marker of antibody colloidal gold containing carbendazim (13), fix in sample pad
There is the cylindricality block across well (2), be in cone at the top of cylindricality block, the colloidal gold pad (8) is resisting carbendazim Antibody Gold
Gold pad (8), the coated film (7) be equipped with conjugate of antigen protein containing carbendazim detection line (6) and one containing resisting
The nature controlling line (5) of rabbit antibody or anti-mouse antibody.
2. carbendazim rapid detection card according to claim 1, it is characterised in that: the coated film (7) is cellulose nitrate
Plain film.
3. carbendazim rapid detection card according to claim 2, it is characterised in that: the nitrocellulose filter both ends difference
With the mutually overlapping connection of resisting carbendazim Antibody Gold gold pad (8) and water absorption pad (4), the sample pad (9) is overlayed in resisting carbendazim
In Antibody Gold gold pad (8).
4. carbendazim rapid detection card according to claim 1, it is characterised in that: round tube (11) diameter is less than sample-adding
Hole (2) diameter.
5. carbendazim rapid detection card according to claim 1, it is characterised in that: it is multiple fixed that the card body (1) is equipped with
Position pin, plate (10) are equipped with the location hole (15) cooperated with positioning pin (14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821296950.3U CN208420936U (en) | 2018-08-13 | 2018-08-13 | A kind of carbendazim rapid detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821296950.3U CN208420936U (en) | 2018-08-13 | 2018-08-13 | A kind of carbendazim rapid detection card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208420936U true CN208420936U (en) | 2019-01-22 |
Family
ID=65122447
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821296950.3U Active CN208420936U (en) | 2018-08-13 | 2018-08-13 | A kind of carbendazim rapid detection card |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208420936U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109813917A (en) * | 2019-02-22 | 2019-05-28 | 山东爱维德生物科技有限公司 | A kind of calprotectin detection kit and its test method |
-
2018
- 2018-08-13 CN CN201821296950.3U patent/CN208420936U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109813917A (en) * | 2019-02-22 | 2019-05-28 | 山东爱维德生物科技有限公司 | A kind of calprotectin detection kit and its test method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Pucher et al. | Determination of ammonia and of amide nitrogen in plant tissue | |
| Udenfriend et al. | Fluorescence characteristics of 5-hydroxytryptamine (serotonin) | |
| CN209167324U (en) | It is a kind of to detect the detecting device integrated of helicobacter pylori in fecal specimens | |
| CN208420936U (en) | A kind of carbendazim rapid detection card | |
| CN106501513B (en) | A kind of Potyvirus Rapid detection test strip and its preparation method and application | |
| CN105588935B (en) | A kind of multinomial urine detection paper box | |
| CN102507931A (en) | Colloidal gold immunochromatographic assay test strip for pyrethroid pesticides and preparation and use methods | |
| CN201464476U (en) | System detecting antibody of schistosomiasis through dot immuno-gold filtration assay | |
| DK142823B (en) | Method and Instrument for Rapid Examination of a Male Patient for Neisseria gonorrheae. | |
| CN110007085A (en) | Rapid detection box and the method that medicinal material is quickly detected using it | |
| CN109307758A (en) | A kind of white blood cell detection kit | |
| CN103675298B (en) | Immune hemolytic anemia detection kit and detection method thereof | |
| CN110161269A (en) | A kind of sample collection device, collection method and detection method | |
| CN209086255U (en) | A kind of white blood cell detection kit | |
| CN209327056U (en) | A kind of corollary apparatus being loaded for the quantitative sampling of micro-fluidic chip and dilution | |
| CN208672649U (en) | A kind of carbofuran rapid detection card | |
| CN205965899U (en) | Laboratory is with test tube of taking sealing device | |
| CN101000342A (en) | Gold-labelled diagnosis test tube of automatic precision micro-quantitative quick sampling | |
| CN101451997A (en) | Reagent strip for rapidly detecting colloidal gold for 2,4-D residual | |
| CN201811921U (en) | Portable rapid determination device for arsenic in water body | |
| CN110161272A (en) | A kind of collecting chamber | |
| CN203053869U (en) | Hemoglobin multichannel detection card | |
| CN208420951U (en) | A kind of portable inspectiont blood group plate | |
| CN214703652U (en) | Card for detecting 6-benzyladenine colloidal gold in bean sprouts | |
| CN206114636U (en) | Multi -functional melamine detects box |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20191028 Address after: 518108 C401, No.2, Industrial Avenue, Buxin village, Buxin community, Dapeng street, Dapeng New District, Shenzhen City, Guangdong Province Patentee after: Shenzhen Jinyu Science and Technology Co., Ltd. Address before: 518120 D building 101.201.301, 2 Industrial Avenue, new community, Dapeng District, Dapeng District, Shenzhen, Guangdong Patentee before: Shenzhen Lvshiyuan Biotechnology Co., Ltd. |