CN208407028U - Fluid path gas path module for micro-fluidic chip detection - Google Patents

Abstract

It include upper sliding block, sample introduction gas circuit column, fluid path column, liquid level bar and valve compression bar the utility model discloses a kind of fluid path gas path module；Sample introduction gas circuit column end is provided with gas circuit sealing element, has sealing ring on gas circuit sealing element；Valve compression bar controls motor control by valve, and valve compression bar control motor and fluid path gas path module control motor are arranged above fluid path gas path module.Sample introduction gas circuit column is blown afloat for positive pressure pushes the sample being added in chip to reach reaction zone, gas circuit sealing element, for guarantee and chip cooperation at sealing reliability, using sealing ring, integrated sealing mode utmostly meets the sample introduction and cleaning seal request of micro-fluidic chip；Fluid path column does not allow it ventilative for the cleaning solution mouth in encapsulating chip；Whether liquid level bar reaches and for incuding sample full of reaction zone；Valve compression bar is for guaranteeing that chip reaction warehouse is reacted full of sample and reagent；Liquid level sensing is carried out using liquid level bar, it can accurate sense micro-fluidic chip fluid flow inside situation.

Description

Fluid path gas path module for micro-fluidic chip detection

Technical field

The utility model belongs to technical field of medical equipment, more particularly, to a kind of fluid path for micro-fluidic chip detection Gas path module.

Background technique

Micro-fluidic chip (microfluidic chip) is current micro-total analysis system (Miniaturized Total Analysis Systems) development hot fields.Microfluidic chip analysis is using chip as operating platform, while with analytical chemistry Based on, it is to rely on micro electro mechanical processing technology, is main application at present with life science using microchannel network as structure feature Object is the emphasis of current micro-total analysis system field development.Its target is the function entire laboratory, including sampling, Dilution, reagent adding, reaction, separation, detection etc. are integrated on microchip.Micro-fluidic chip is the main flat of microflow control technique realization Platform.Its device characteristic is mainly that its resulting structure (channel, reaction chamber and other certain functional components) for accommodating fluid at least exists It is micron order scale on one latitude.Due to micron-sized structure, fluid shows and produces wherein different from macro-scale Property.Therefore the performance that unique analysis generates is developed.The characteristics of micro-fluidic chip and advantages for development: micro-fluidic core Piece has the characteristics that liquid flowing controllable, consumption sample and reagent are few, improve to analysis speed tenfold hundreds of times, it can be with Analysis while carrying out a sample up to a hundred within a few minutes or even shorter time, and can be with the pretreatment of canbe used on line sample And analysis overall process.Its application purpose generated is to realize ultimate aim-chip lab of micro-total analysis system, at present work The focus on the application field for making to develop is life science.

In Chinese patent literature CN107843583A, a kind of micro-fluidic chemiluminescent analyzer and user are disclosed Method, including fluid path gas path module, chip loading module, ultrasonic module, fluid path gas path module, chip loading module, ultrasonic module Sequence is arranged.The utility model collection is multiple to be installed on one, can carry out barcode scanning to each chip, determine personalization detection Mesh；Sample, reaction reagent, luminous substrate are mixed well using ultrasonic module, finally carries out optical detection, data is read, makes Entire detection program standardization, automation, and detection sensitivity is high, result is reliable.The utility model is guaranteeing every detection function On the basis of energy, structure has carried out great simplification, and small volume, manufacturing cost is low, easy to operate, be suitble to patient at one's side into Row real-time test can be quickly obtained testing result.

But above-mentioned technical proposal for ensuring that chip reaction warehouse is reacted full of sample and reagent, do not give by fluid path gas path module Ideal solution out, therefore, it is necessary to a kind of new fluid path gas path module.

Utility model content

Problem to be solved in the utility model is to provide a kind of structure novel, easy to operate, it is ensured that chip reaction warehouse fills The fluid path gas path module for micro-fluidic chip detection of full sample and reagent reaction.

In order to solve the above technical problems, the technical solution adopted in the utility model is, on which includes Sliding block, sample introduction gas circuit column, fluid path column, liquid level bar and valve compression bar；The sample introduction gas circuit column end is provided with gas circuit sealing element, There is sealing ring on the gas circuit sealing element；The valve compression bar controls motor control by valve, and the valve compression bar controls horse It is arranged above the fluid path gas path module up to fluid path gas path module control motor.

The setting of upper sliding block, the direction and position pushed to chip for ensuring fluid path gas path module is accurate, into Sample gas circuit column is blown afloat for positive pressure pushes the sample being added in chip to reach reaction zone, gas circuit sealing element, for guaranteeing device Sealing reliability at (fluid path gas path module) and chip cooperation, using sealing ring (flexible, such as rubber material), integration Sealing means utmostly meet the sample introduction and cleaning seal request of micro-fluidic chip；Fluid path column is for clear in encapsulating chip Washing lotion mouth does not allow it ventilative；Whether liquid level bar reaches and for incuding sample full of reaction zone；Valve compression bar is used to work as liquid level bar Sense that sample reaches and be full of reaction zone, valve control motor is made immediately closes valve response, and valve compression bar kills Valve guarantees that chip reaction warehouse is reacted full of sample and reagent；Using liquid level bar carry out liquid level sensing, can accurate sense it is micro-fluidic Chip interior liquid mobility status, grasps the volume of sample and cleaning solution in micro-fluidic chip in time, and can judge automatically miniflow Control chip pipeline blockage situation.

Preferably, multiple tracks muscle bridge structure is used on sealing ring semi arch；Multiple tracks muscle bridge setting, so that it is easier by crowded Compressive strain starts sealing function.

Preferably, which has upper bracket；The valve compression bar control motor and the fluid path gas circuit mould Block control motor is arranged on upper bracket；Lower bracket is flexibly connected by the upper sliding block with the upper bracket, wherein institute Sample introduction gas circuit column, fluid path column and liquid level bar is stated to be installed on lower bracket；It is compact-sized, it installs, is easy to operate.

Preferably, the fluid path gas path module control motor is lead screw motor, controls motor with the fluid path gas path module On the feed screw nut that is mutually equipped with of screw rod be fixedly mounted on the lower bracket, drive the lower bracket to slide up and down.

Preferably, the lower bracket is divided into two layers, is upper end surface layer and lower end surface layer respectively, the upper end surface layer is for pacifying The feed screw nut is filled, and also there is a notch in the upper end surface layer；The valve compression bar is actively plugged in lower end In surface layer, the fluid path column is also connected with liquid pump, and the sample introduction gas circuit column is also connected with air pump.

Preferably, lightening hole is additionally provided on the upper bracket.

Another technical problem to be solved in the utility model be to provide it is a kind of take into account micro-fluidic chip and NC film detection Technology application scene meets micro-fluidic and NC film and detects the more for micro-fluidic chip and NC film of two kinds of measuring tables sample introduction simultaneously The device of flux detection all-in-one machine.

In order to solve the above technical problems, the technical solution adopted in the utility model is, this is used for micro-fluidic chip and NC film Multi-pass amount detect all-in-one machine device, include pedestal, be provided on the pedestal sample introduction module, incubate module, cleaning module, NC film detection module and chip detection module；

Wherein, it is provided with mutually independent chip sample introduction card slot and NC film sample introduction card slot in the sample introduction module, uses respectively In insertion chip or NC film strip；Fluid path gas path module is additionally provided in the sample introduction module；

There is mutually independent chip to incubate slot and NC film in the incubation module and incubate slot, and is the sample introduction module, clear The side of mold cleaning block and NC film detection module on the incubation block length direction is arranged；It is cleaned in the cleaning module Chip afterwards send into the chip detection module and is detected.

Fluid path gas path module in sample introduction module, for ensuring that chip reaction warehouse is reacted full of sample and reagent；Chip temperature Slot is educated, for carrying out constant-temperature incubation to chip；Similarly, NC film incubates slot, for carrying out constant-temperature incubation to NC film strip；Using upper Technical solution is stated, modules component is incorporated on pedestal and forms complete equipment, it is structure-integrated, it can especially take into account The Technology application scene of micro-fluidic chip and the detection of NC film, micro-fluidic chip detection have precision high, and speed is fast, testing cost compared with High feature is appropriate for the detection that accurate medical rings carry out, and the detection of NC film has speed fast, and precision is low, and testing cost is low The characteristics of, it is generally used for the detection of screening type；It all can go to detect with high-precision methodology to avoid any project, lead to medical money It the advantages of the problem of overconsumption in source, technology that there are two types of tools, can rationally be detected with the actual conditions of R. concomitans scene, The organic unity for reaching Social benefit and economic benefit can use NC film lateral chromatography for the less high project of sensitivity requirement Method solves, and can not only reduce cost, but also flux can be improved；However and stability requirement especially high to sensitivity requirement is also very High, highly difficult project can be detected with microfluidic methods, that is, sensitivity can be improved, also can reach accuracy.Furthermore in this reality With in new technique scheme, micro-fluidic chip and the detection of NC film are mutually indepedent, do not interfere with each other, and can meet while two kinds of measuring tables Sample introduction simultaneously meets the cooperation demand of two methods during the system detection of POCT detection process, improves detection efficiency.

Preferably, the side of the chip sample introduction card slot is provided with chip sample introduction stirring hand, the chip sample introduction stirring hand is solid Surely it is connected on the first transmission connection block, the first transmission connection block passes through synchronization by the chip sample introduction stirring hand power motor Band driving moves back and forth；The side of the NC film sample introduction card slot is provided with NC film sample introduction stirring hand, the NC film sample introduction stirring hand is solid Surely it is connected on the second transmission connection block, the second transmission connection block passes through synchronization by the NC film sample introduction stirring hand power motor Band driving moves back and forth.

Chip is inserted into chip sample introduction card slot, chip sample introduction card slot enters in the device of the utility model all-in-one machine Chip is moved in the fluid path gas path module in sample introduction module by portion, in-position, by fluid path gas path module to joined sample This chip is handled；The side of chip sample introduction card slot is arranged in chip sample introduction stirring hand, so that chip sample introduction stirring hand controls horse Constant-temperature incubation is carried out up to driving chip sample introduction stirring hand to dial in chip in chip incubation slot；By NC film strip be inserted into NC film into In scale slot, NC film sample introduction card slot enters inside the device of the utility model all-in-one machine, in-position, NC film sample introduction stirring hand power Motor drives NC film sample introduction stirring hand to dial in NC film strip in NC film incubation slot and carries out constant-temperature incubation.

Preferably, the first transmission draw runner is provided below in the first transmission connection block；Under the second transmission connection block Face is provided with the second transmission draw runner；The chip sample introduction card slot controls motor control, the NC film sample introduction by chip sample introduction card slot Card slot controls motor control by NC film sample introduction card slot, and the fluid path gas path module is carried out by fluid path gas path module control motor control Movement.

Transmission connection block is moved back and forth along transmission draw runner, and position is accurate, mobile to stablize, and will not generate shaking；It adopts Motor-driven control chip provided sample introduction card slot is controlled with chip sample introduction card slot, motor control NC film sample introduction card is controlled using NC film sample introduction card slot Slot, keeps independence between the two, chip, NC film strip enter inside the device of the utility model all-in-one machine it is more convenient, It is convenient；Fluid path gas path module is pushed by fluid path gas path module control motor control, and then is handled chip.

Preferably, in the incubation module, the chip incubates slot and NC film incubates slot closed assembly setting up and down；The core Piece incubates slot, and there is at least one slot position to be used for chip placement, and the chip incubates slot and controls motor control by chip incubation slot, So that the chip incubation slot is free to slide along chip incubation slot polished rod；The NC film incubates slot, and there is at least one slot position to be used for NC film strip is placed, the NC film incubates slot and incubates slot control motor control by NC film, so that the NC film incubates slot along NC film It is free to slide to incubate slot polished rod.

Chip incubates slot and NC film incubates slot closed assembly setting up and down, compact-sized, and embodies the independence of the two, not phase Interference；It is free to slide along chip incubation slot polished rod that chip incubates slot, and sample introduction module, cleaning module and chip detection module are along institute The side setting incubated on block length direction is stated, facilitates and dials in the chip being located in sample introduction module in chip incubation slot, Also the chip that will be located in chip incubation slot is facilitated to dial in cleaning module；Likewise, NC film, which incubates slot, incubates slot along NC film Polished rod is free to slide, and the side of sample introduction module, cleaning module and NC film detection module on the incubation block length direction Setting facilitates and dials in NC film strip in NC film incubation slot, and the NC film strip that NC film incubates in slot will be located at by, which also facilitating, push In NC film detection module；Slot position can be set multiple, and the synchronous detection of multi-chip or more NC film strips can meet multiple chips, more NC film strip Synchronous Transmission in systems, meets the needs of detection system is to flux and efficiency.

Preferably, the cleaning module has chip rinse bath, the chip rinse bath and chip cleaning transmission connection block It is fixedly connected, the synchronous belt that control motor driving is driven by chip rinse bath is fixedly mounted in the chip cleaning transmission connection block On, the chip rinse bath moves back and forth in linear guide；The cleaning module controls motor control by cleaning fluid path gas circuit, And the cleaning fluid path gas circuit control motor is arranged above the cleaning module.

In the present invention, cleaning module is identical as fluid path gas path module on specific structure, cleans the control of fluid path gas circuit Motor control cleaning module processed pushes, cleaning module using structure same with fluid path gas path module design (gas circuit sealing element) come Guarantee air-tightness, cleaning solution is beaten using liquid pump positive pressure and is cleaned, cleaning for several times, has been beaten cleaning solution every time, controlled using air pump The cleaning solution in barotropic gas emptying chip reaction zone is blown, until cleaning is completed；It is gone the same way mode using gas-liquid, utilizes a sample introduction Mouth and valve pump control system can meet specific demand of the micro-fluidic chip to gas-liquid sample introduction in conjunction with microcomputer control sequential.

Preferably, the NC film detection module includes NC film light path, is provided with below the NC film light path NC film test card slot detects NC film light path described in control motor control by NC film and moves above the NC film test card slot It is dynamic；The side of the NC film test card slot is provided with limited post, the NC film test card slot as described in discarding strip control motor control It is mobile.

After NC film strip is pushed in NC film test card slot from NC film incubation slot, NC film detection control motor control NC film Light path is moved forward and backward acquisition signal above NC film test card slot, is analyzed, after detection, is controlled by discarding strip Motor control NC film test card slot moves right, and the side of NC film test card slot is provided with limited post, and limitation NC film strip is to the right Mobile, when NC film test card slot is moved to certain position, NC film strip falls down NC film test card slot, and it is dynamic that strip is abandoned in completion Make.

Preferably, the NC film gone out in sample detection means by NC film goes out sample stirring hand and NC film strip is incubated slot from the NC film In push in the NC film test card slot.

Preferably, the chip detection module includes excitation module, is provided with sending and receiving below the excitation module Optical port is penetrated, mobile by barn door drive motor driving barn door, the lower section that optical port is penetrated in the sending and receiving is arranged in the barn door；Position Photomultiplier tube PMT is additionally provided in the bottom of the chip detection module；Barn door is set, prevents side light source to detection Interference, improves the sensitivity of optical detection.

Preferably, it is connected with turntable on the output shaft of the barn door drive motor, has on the turntable and stirs column, institute It states and stirs column and be inserted into the groove of the barn door.

By the rotation of turntable, drives and stir column movement on turntable, so that barn door moves left and right.

Detailed description of the invention

It is further described with reference to the accompanying drawing with the embodiments of the present invention:

Fig. 1 is apparatus structure schematic diagram of the utility model for micro-fluidic chip and NC film multi-pass amount detection all-in-one machine；

Fig. 2 is the sample introduction module structural schematic diagram in Fig. 1；

Fig. 3 is the sample introduction module backsight structural representation in Fig. 2；

Fig. 4 is the fluid path gas path module structural schematic diagram in the utility model；

Fig. 5 is the gas circuit seal configuration schematic diagram in Fig. 4；

Fig. 6 is the incubation modular structure schematic diagram in Fig. 1；

Fig. 7 is the cleaning module structural schematic diagram in Fig. 1；

Fig. 8 is the NC film detection module structural schematic diagram in Fig. 1；

Fig. 9 is the chip detection module structural schematic diagram in the utility model；

Figure 10 is the detonation configuration figure of Fig. 4；

Wherein: 1- pedestal；2- sample introduction module；201- chip sample introduction card slot；202-NC film sample introduction card slot；203- chip sample introduction Stirring hand；The transmission connection block of 204- first；205- chip sample introduction stirring hand power motor；206-NC film sample introduction stirring hand；207- second is passed Dynamic link block；208-NC film sample introduction stirring hand power motor；209- first is driven draw runner；2010- second is driven draw runner；2011- core Piece sample introduction card slot controls motor；2012-NC film sample introduction card slot controls motor；3- incubates module；301- chip incubates slot；302-NC Film incubates slot；303-NC film incubates slot and controls motor；304- chip incubates slot and controls motor；305- chip incubates slot polished rod； 306-NC film incubates slot polished rod；307- driving block；308- bracket；309- skids off mouth；4- cleaning module；The cleaning of 401- chip Slot；402- chip cleaning transmission connection block；403- chip rinse bath transmission control motor；404- linear guide；405- cleaning solution Road gas circuit controls motor；5-NC film detection module；501-NC film light path；502-NC film test card slot；The detection of 503-NC film Control motor；504- limited post；505- abandons strip and controls motor；506- detection platform；507- resisting piece；508-NC film strip Rest area；509-NC film strip falls into mouth；5010- surrounding edge；5011- limits post holes；5012-NC film detects sliding block；The inspection of 6- chip Survey module；601- excitation module；Optical port is penetrated in 602- sending and receiving；603- barn door drive motor；604- barn door；605- photoelectricity times Increase pipe PMT；606- output shaft；607- turntable；608- stirs column；609- groove；7- fluid path gas path module；701- fluid path gas circuit mould Block controls motor；The upper sliding block of 702-；703- sample introduction gas circuit column；704- fluid path column；705- liquid level bar；706- valve compression bar； 707- gas circuit sealing element；708- sealing ring；709- valve controls motor；7010- upper bracket；7011- lower bracket；7012,7018- Feed screw nut；The upper end 7013- surface layer；The lower end 7014- surface layer；7015- notch；7016,7017- screw rod；7019- lightening hole； 7020- guide rail；8-NC film goes out sample detection means；801-NC film goes out sample stirring hand.

Specific embodiment

As shown in Figure 1, being the device knot for micro-fluidic chip and NC film multi-pass amount detection all-in-one machine of the utility model Structure schematic diagram includes pedestal 1, and sample introduction module 2 is provided on the pedestal 1, incubates module 3, cleaning module 4, the detection of NC film Module 5 and chip detection module 6；

Wherein, mutually independent chip sample introduction card slot 201 and NC film sample introduction card slot 202 are provided in the sample introduction module 2, It is respectively for insertion into chip or NC film strip；Fluid path gas path module 7 is additionally provided in the sample introduction module 2；

There is mutually independent chip to incubate slot 301 and NC film incubation slot 302, and the sample introduction in the incubation module 3 The side of module 2, cleaning module 4 and NC film detection module 5 on incubation 3 length direction of module is arranged；In the cleaning Chip after being cleaned in module 4, which is sent into the chip detection module 6, to be detected.

As shown in Figure 2,3, the side of the chip sample introduction card slot 201 is provided with chip sample introduction stirring hand 203, the chip Sample introduction stirring hand 203 is fixedly connected on the first transmission connection block 204, and the first transmission connection block 204 is by the chip sample introduction Stirring hand power motor 205 is moved back and forth by synchronous belt driving；The side of the NC film sample introduction card slot 202 be provided with NC film into Sample stirring hand 206, the NC film sample introduction stirring hand 206 are fixedly connected on the second transmission connection block 207, the second transmission connection block 207 are moved back and forth by the NC film sample introduction stirring hand power motor 208 by synchronous belt driving.

The first transmission draw runner 209 is provided below in the first transmission connection block 204；The second transmission connection block 207 The second transmission draw runner 2010 is provided below；The chip sample introduction card slot 201 is controlled by chip sample introduction card slot control motor 2011, The NC film sample introduction card slot 202 is controlled by NC film sample introduction card slot control motor 208, and the fluid path gas path module 7 is by fluid path gas circuit The module control control of motor 701 is acted.

As shown in figure 4, the fluid path gas path module 7 includes upper sliding block 702, sample introduction gas circuit column 703, fluid path column 704, Liquid level bar 705 and valve compression bar 706；The end of the sample introduction gas circuit column 703 is provided with gas circuit sealing element 707, and the gas circuit is close There is sealing ring 708 (as shown in Figure 5) on sealing 707, in the present embodiment, the material of sealing ring 708 is rubber, realizes soft connect It touches, multiple tracks muscle bridge structure is used on sealing ring semi arch, muscle bridge is squeezed deformation, starts sealing function；The valve compression bar 706 are controlled by valve control motor 709, and the valve compression bar control motor 709 and the fluid path gas path module control motor 701 It is arranged at 7 top of fluid path gas path module.

In the present embodiment, the end of fluid path column 704 is also to be provided with gas circuit sealing element 707, on gas circuit sealing element 707 Sealing ring 708 is installed；In addition, with regard to the fluid path gas path module 7 of the present embodiment, specifically, there are also upper bracket 7010, valve compression bar Control motor 709 and fluid path gas path module control motor 701 are arranged on upper bracket 7010, and lower bracket 7011 passes through upper downslide Block 702, and the guide rail 7020 being equipped with upper 702 phase of sliding block are flexibly connected with upper bracket 7010, wherein sample introduction gas circuit column 703, fluid path column 704 and liquid level bar 705 are installed on lower bracket 7011, and it is screw rod electricity that fluid path gas path module, which controls motor 701, The feed screw nut 7012 that 7016 phase of screw rod on machine, with fluid path gas path module control motor 701 is equipped with is fixedly mounted on lower bracket On 7011, driving lower bracket 7011 is slided up and down, and it is upper end surface layer 7013 and lower end surface layer respectively that it is two layers that lower bracket 7011, which divides, 7014, upper end surface layer 7013 is used to install feed screw nut 7012, and also has a notch 7015 in upper end surface layer 7013, The equipped feed screw nut 7018 of 7017 phase of screw rod for giving valve control motor 709 reserves installation site, 706 activity of valve compression bar It is inserted into lower end surface layer 7014, fluid path column 704 is also connected with liquid pump, and sample introduction gas circuit column 703 is also connected with air pump；Shown in Figure 10 Be above-mentioned fluid path gas path module 7 detonation configuration figure, the as can be seen from the figure more detailed structure of fluid path gas path module 7, on Lightening hole 7019 is additionally provided on bracket 7010.

In the incubation module 3, as shown in fig. 6, the chip incubates slot 301 and NC film incubates about 302 closed assembly of slot Setting；The chip incubates slot 301, and there is at least one slot position to be used for chip placement (in the present embodiment, having 8 slot positions), The chip incubates slot 301 and is controlled by chip incubation slot control motor 304, so that the chip incubates slot 301 and incubates along chip Slot polished rod 305 is free to slide；The NC film, which incubates slot 302, has at least one slot position for placing NC film strip (in this implementation In example, and there are 8 slot positions), the NC film incubates slot 302 and is controlled by NC film incubation slot control motor 303, so that the NC It is free to slide along NC film incubation slot polished rod 306 that film incubates slot 302.

In incubating module 3, there is bracket 308, NC film incubates slot 302 and is located above, and there are two NC films to incubate slot light Bar 306 is incubated in two NC films there are synchronous belt installation space between slot polished rod 306, and NC film incubates slot control motor 303 and connects There is synchronous belt, NC film incubates slot 302 and driving block 307 is provided below, and driving block 307 and NC film incubate slot and control motor The synchronous belt of 303 drivings is fixedly connected；Chip incubates slot 301 and is located at the lower section that NC film incubates slot 302, structure and above-mentioned NC film temperature It educates that slot 302 is identical, in addition, on the right side of bracket 308, and with chip incubates the corresponding position of slot 301 and be provided with and skid off mouth 309, The chip that chip is incubated incubation time completion in slot 301 is facilitated to be allocated in chip rinse bath 401.

Cleaning module 4 has chip rinse bath 401, and the chip rinse bath 401 and chip cleaning transmission connection block 402 are solid Fixed connection, the chip cleaning transmission connection block 402 be fixedly mounted on by chip rinse bath be driven control motor 403 drive it is same Step takes, and the chip rinse bath 401 moves back and forth in linear guide 404；The cleaning module 4 is by cleaning fluid path gas circuit control Motor 405 processed controls, and cleaning fluid path gas circuit control motor 405 is arranged above the cleaning module 4, as shown in Figure 7.

The NC film detection module 5 of the utility model as shown in Fig. 8, includes NC film light path 501, the NC film It is provided with NC film test card slot 502 below light path 501, control motor 503 is detected by NC film and controls the NC film detection Optical path 501 moves above the NC film test card slot 502；The side of the NC film test card slot 502 is provided with limited post 504, it is mobile that the NC film test card slot 502 is controlled by discarding strip control motor 505.

Specifically, NC film detection module 5 includes detection platform 506, and the edge of detection platform 506 is provided with resisting piece 507, Resisting piece 507 and NC film test card slot 502 are surrounded by NC film strip rest area 508；Below NC film strip rest area 508, and It is provided with NC film strip in detection platform 506 and falls into mouth 509；Surrounding edge 5010 in NC film test card slot 502 has limit Post holes 5011；NC film light path 501 is fixedly connected with NC film detection sliding block 5012, and NC film detection sliding block 5012 is detected by NC film The control movement of motor 503 is controlled, and then NC film light path 501 is driven to move above NC film test card slot 502.

In Fig. 1, we can further be seen that the NC film gone out in sample detection means 8 by NC film goes out sample stirring hand 801 for NC film Strip is incubated in slot 302 from the NC film and is pushed in the NC film test card slot 502.

Shown in Fig. 9 is chip detection module 6, includes excitation module 601, is set below the excitation module 601 It is equipped with sending and receiving and penetrates optical port 602, drive barn door 604 mobile by barn door drive motor 603, the barn door 604 is arranged in institute The lower section that optical port 602 is penetrated in sending and receiving, mountable different optical filter on barn door 604 are stated, barn door controls the mobile shading of motor 603 Plate 604 carries out different optical wavelength and receives；Bottom positioned at the chip detection module 6 is additionally provided with photomultiplier tube PMT605, chip is placed on excitation module 601 and sending and receiving are penetrated between optical port 602；The output shaft of barn door drive motor 603 It is connected with turntable 607 on 606, has on the turntable 607 and stirs column 608, the column 608 of stirring is inserted into the barn door In 604 groove 609.

When specifically used:

(1) NC film strip is detected: NC film strip is inserted into NC film sample introduction card slot 202, NC film sample introduction card slot It controls the control NC film sample introduction card slot 202 of motor 2012 to enter inside device, reaches predetermined position, NC film sample introduction stirring hand power motor NC film strip is dialled in NC film incubation slot 302 and carries out constant-temperature incubation by 208 drive NC film sample introduction stirring hands 206, and incubation time is complete At NC film incubates slot control motor 303 and NC film incubation slot 302 is driven to move to designated position, and NC film goes out sample stirring hand 801 for NC film Strip is pushed from the slot position that NC film incubates slot 302 in the NC film test card slot 502 in NC film detection module 5, the detection control of NC film Motor 503 processed controls NC film light path 501 and is moved forward and backward acquisition signal, is analyzed, and after detection, abandons strip control Motor 505 controls NC film test card slot 502 and moves right, and limited post 504 passes through in the surrounding edge 5010 of NC film test card slot 502 Post holes 5011 is limited, limitation NC film strip moves right, when NC film test card slot 502 continues to move to certain position, NC film Strip falls down NC film test card slot 502 from NC film strip rest area 508, falls into mouth 509 into NC film strip, NC film is abandoned in completion Strip movement；

(2) micro-fluidic chip is detected: the chip that sample has been added is inserted into chip sample introduction card slot 201, core Piece sample introduction card slot controls the control chip sample introduction card slot 201 of motor 2011 and enters inside device, reaches predetermined position, fluid path gas circuit mould Block controls motor 701 and controls the pushing of fluid path gas path module 7, guarantees the sealing at device (fluid path gas path module 7) and chip cooperation Reliability, fluid path column 704 seal the cleaning solution mouth on chip, and sample introduction gas circuit column 703 pushes down the injection port on chip, then, into Sample gas circuit column 703 injection port positive pressure blow afloat push sample reach reaction zone, at this moment, liquid level bar 705 sense sample reach and Full of reaction zone, valve control motor 709 is made immediately closes valve response, and valve compression bar 706 kills valve, guarantees core Piece reaction warehouse is reacted full of sample and reagent；Positioned at 2 bottom of sample introduction module ultrasonic module (existing) upwards contact chip into Row ultrasound mixes, and guarantees the abundant reaction of reagent and sample；Chip sample introduction stirring hand power motor 205 drives chip sample introduction stirring hand Chip is dialled in chip by 203 to be incubated in slot 301 and carries out constant-temperature incubation, and incubation time is completed；Chip incubates slot and controls motor 304 It drives chip to incubate slot 301 and moves to designated position, chip is pushed in chip rinse bath 401, the transmission control of chip rinse bath Motor 403 is mobile to indirectly control chip rinse bath 401, is moved to immediately below cleaning liquid circuit module, cleans the control of fluid path gas circuit Motor 405 processed controls cleaning module 4 and pushes, and cleaning module 4 guarantees gas using designing with the same structure of fluid path gas path module 7 Close property, then, liquid pump positive pressure is beaten cleaning solution and is cleaned, and cleaning for several times, has beaten cleaning solution every time, and barotropic gas is blown in air pump control The cleaning solution in chip reaction zone is emptied, cleaning is completed, and chip rinse bath 401 moves backward to chip detection module 6 and carries out light Road acquisition process (chip is placed on excitation module 601 and sending and receiving are penetrated between optical port 602), after data acquire, by chip It loses.

Above-mentioned two detecting step can be carried out individually, can also be carried out simultaneously；In the present embodiment, it can also use micro- Fluidic chip testing process monitoring system, the real-time reaction process of real time monitoring micro-fluidic chip and detection time, meet and use Person understands detection progress, and system can enter instrument and start first time of detection in chip distributes the detection management shelves of chip automatically Case knows chip project and parameter curve by chip two dimensional code, thus display chip time of measuring and remaining detection time, User is facilitated to grasp detection procedure in real time；Automatically the consumptive material that will be generated in NC film and micro-fluidic chip detection process separately abandons, It stores classifiedly；Dual area sample mode improves detection accuracy and stability, cleverly meet micro-fluidic chip to sampling precision and The requirement of stability.

The basic principles and main features and advantage of the utility model have been shown and described above.The technical staff of the industry It should be appreciated that the present utility model is not limited to the above embodiments, the above embodiments and description only describe this The principle of utility model, on the premise of not departing from the spirit and scope of the utility model, the utility model also has various change And improvement, such as some adjustment are done for the position for being mounted on the base all parts, these changes and improvements both fall within requirement In the scope of the utility model of protection.The utility model requires protection scope is by appended claims and its equivalent circle It is fixed.

Claims (6)

1. a kind of fluid path gas path module for micro-fluidic chip detection, which is characterized in that the fluid path gas path module includes There are upper sliding block, sample introduction gas circuit column, fluid path column, liquid level bar and valve compression bar；It is close that the sample introduction gas circuit column end is provided with gas circuit Sealing has sealing ring on the gas circuit sealing element；The valve compression bar controls motor control, the valve compression bar control by valve Motor processed and fluid path gas path module control motor are arranged above the fluid path gas path module.

2. the fluid path gas path module according to claim 1 for micro-fluidic chip detection, which is characterized in that sealing ring half Multiple tracks muscle bridge structure is used on circular arc.

3. the fluid path gas path module according to claim 1 for micro-fluidic chip detection, which is characterized in that the fluid path gas Road module has upper bracket；The valve compression bar control motor and fluid path gas path module control motor are arranged at upper bracket On；Lower bracket is flexibly connected by the upper sliding block with the upper bracket, wherein the sample introduction gas circuit column, fluid path column and liquid Position bar is installed on lower bracket.

4. the fluid path gas path module according to claim 3 for micro-fluidic chip detection, which is characterized in that the fluid path It is lead screw motor that gas path module, which controls motor, the feed screw nut being mutually equipped with the screw rod on fluid path gas path module control motor It is fixedly mounted on the lower bracket, the lower bracket is driven to slide up and down.

5. the fluid path gas path module according to claim 4 for micro-fluidic chip detection, which is characterized in that the lower branch Frame is divided into two layers, is upper end surface layer and lower end surface layer respectively, and the upper end surface layer is used to install the feed screw nut, and in institute Stating also has a notch in the surface layer of upper end；The valve compression bar is actively plugged in the surface layer of lower end, and the fluid path column is also connected with There is liquid pump, the sample introduction gas circuit column is also connected with air pump.

6. the fluid path gas path module according to claim 5 for micro-fluidic chip detection, which is characterized in that the upper branch Lightening hole is additionally provided on frame.