CN208255123U - A kind of bioelectricity pole plate, bioelectronics detection device and biologic electrode array - Google Patents
A kind of bioelectricity pole plate, bioelectronics detection device and biologic electrode array Download PDFInfo
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- CN208255123U CN208255123U CN201820552473.6U CN201820552473U CN208255123U CN 208255123 U CN208255123 U CN 208255123U CN 201820552473 U CN201820552473 U CN 201820552473U CN 208255123 U CN208255123 U CN 208255123U
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Abstract
The utility model discloses a kind of bioelectricity pole plate, bioelectronics detection device and biologic electrode array.The bioelectricity pole plate includes: bioelectrode substrate, the bioelectrode for being fixed on bioelectrode substrate front side, positioned at the electrode contact of bioelectrode substrate back side；The quantity of electrode contact and the quantity of bioelectrode are identical；Each electrode contact is corresponding with the position of corresponding bioelectrode；Each bioelectrode is connected with corresponding electrode contact by penetrating the conductive material of bioelectrode substrate.Bioelectricity pole plate, bioelectronics detection device and biologic electrode array disclosed by the utility model, can enhance interface suitability.
Description
Technical field
The utility model relates to field of biosensors, more particularly to a kind of bioelectricity pole plate, bioelectronics detection dress
It sets and biologic electrode array.
Background technique
Biosensor (biosensor) is to make recognition component and physical and chemical energy converter group appropriate using biological sensitive materials
At a kind of bioelectronics analysis device and detection device, core component is bioelectrode, including enzyme electrode, immunoelectrode,
Nucleic acid electrode, cell electrode, tissue electrode etc. are widely used in medicine detection, residential care, food safety, industrial process, agriculture
The fields such as industry application, environment pollution control, military biochemical protection.
Currently, biosensor has developed to the third generation analytical technology stage based on direct bioelectrode, feature is
It is direct electron transfer process between biosensor and electrode, closer to the archetype of organism chemical reaction system.
The research and application of direct biosensor can not only obtain the dynamics of biochemical reaction and the important application ginseng of mechanical process
Number, and people are understood with the structure and various physicochemical properties of biomolecule machine, explore enzyme, antigen-antibody, aptamer
Etc. physiological action and mechanism of action of the biomolecule in life entity all have great importance.
Fig. 1 is the structure chart of direct biosensor in the prior art.Referring to Fig. 1, which includes
Positioned at the bioelectrode slot of electrode holder upper surface, electrode cable slot, the bioelectrode on bioelectrode slot and it is located at electricity
One end of electrode cable on polar conductor slot, each electrode cable slot is respectively connected to a bioelectrode slot, the other end
Electrode holder edge is all extended to, each electrode cable is all connected to the electrode contact on the outside of electrode holder, each biology
One bioelectrode is set in slot electrode, is connected to corresponding electrode cable.Bioelectrode include auxiliary electrode, working electrode and
Reference electrode.Sample is added dropwise on bioelectrode surface when measurement, electrode contact insertion biological respinse detecting instrument connects
Mouthful, which can only realize disposable grafting, and interface suitability is poor.
Summary of the invention
The purpose of the utility model is to provide a kind of bioelectricity pole plate, bioelectronics detection device and biologic electrode array,
Improve interface suitability.
To achieve the above object, the utility model provides following scheme:
A kind of bioelectricity pole plate, comprising: bioelectrode substrate, the bioelectricity for being fixed on the bioelectrode substrate front side
Pole, positioned at the electrode contact of the bioelectrode substrate back side；The quantity of the quantity of the electrode contact and the bioelectrode
It is identical；Each electrode contact is corresponding with the position of corresponding bioelectrode；Each bioelectrode with it is corresponding
Electrode contact is connected by penetrating the conductive material of the bioelectrode substrate.
Optionally, the quantity of the bioelectrode and the electrode contact is three；Three bioelectrode difference
For auxiliary electrode, working electrode and reference electrode.
Optionally, three electrode contacts are equidistantly arranged in a row；Be located in the middle the electrode contact with it is described
Working electrode connection, two electrode contacts positioned at both ends are connect with the auxiliary electrode and the reference electrode respectively.
Optionally, the shape of the working electrode is circle, positioned at the center of the bioelectrode substrate；The auxiliary electricity
Pole and the reference electrode are positioned at the working electrode periphery and using the center of circle of the working electrode as the arc in the center of circle.
Optionally, the material of the bioelectrode substrate is polytetrafluoroethylene (PTFE), ceramics or glass.
Optionally, the conductive material is gold, platinum gold, silver, copper, carbon nanotube or graphene.
It further includes anti-that the invention also discloses a kind of bioelectronics detection devices including above-mentioned bioelectricity pole plate
Ying Chi, blender and electrode bar；The reaction tank is circular cylindrical cavity；It is provided on the reaction tank side wall and the bioelectricity
The corresponding electrode interface of the electrode contact of pole plate；The bioelectricity pole plate is attached on the reaction tank side wall and the bioelectricity
Each electrode contact of pole plate is respectively corresponded in each electrode interface, and one end of the electrode bar is provided with and each electrode interface
Corresponding electrode contact, each electrode contact is connected on corresponding electrode contact by the electrode interface, each
The electrode contact is connect with the conducting wire inside the electrode bar, and the conducting wire is connected in biological respinse analysis instrument；
The blender is arranged in the reaction bottom of pond portion；
Liquid is additionally provided in the reaction bottom of pond portion and enters pipeline, is provided with liquid outlet tube at the top of the reaction tank
Road and sample holes.
A kind of biologic electrode array is also disclosed in the utility model, including multiple above-mentioned bioelectricity pole plates, multiple lifes
Object electrode plate is equidistantly arranged in multiple lines and multiple rows.
According to specific embodiment provided by the utility model, the utility model discloses following technical effects: this is practical new
Bioelectricity pole plate, bioelectronics detection device disclosed in type and biologic electrode array, bioelectrode and electrode contact are distributed in
Bioelectrode substrate two sides, so as to make electrode that with biological respinse analysis instrument grafting, it is suitable more easily to improve interface
With property.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Needed in attached drawing be briefly described, it should be apparent that, the accompanying drawings in the following description is only the utility model
Some embodiments for those of ordinary skill in the art without any creative labor, can also basis
These attached drawings obtain other attached drawings.
Fig. 1 is the structure chart of direct biosensor in the prior art；
Fig. 2 is the Facad structure figure of the utility model bioelectricity pole plate embodiment；
Fig. 3 is the inverse layer structure figure of the utility model bioelectricity pole plate embodiment；
Fig. 4 is the structure drawing of device of the utility model bioelectronics detection device embodiment；
Fig. 5 is bioelectricity pole plate and electrode bar connection schematic diagram in the utility model bioelectricity force checking device embodiment；
Fig. 6 be the utility model by 12 bioelectrode board groups at biologic electrode array embodiment structure chart；
Fig. 7 be the utility model by 48 bioelectrode board groups at biologic electrode array embodiment structure chart.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
Fig. 1 is the structure chart of direct biosensor in the prior art.Direct biosensor presence can only be realized
Disposable grafting, the problem of interface suitability difference.The reason of finding after study, causing the problem is that the biosensor is
Bioelectrode and electrode contact are arranged at the same face of substrate by single side biosensor.Therefore the utility model passes through
Change this design in the prior art to provide it is a kind of improve interface suitability bioelectricity pole plate and bioelectronics detection
Device.
To keep the above objects, features, and advantages of the utility model more obvious and easy to understand, with reference to the accompanying drawing and have
Body embodiment is described in further detail the utility model.
Fig. 2 is the Facad structure figure of the utility model bioelectricity pole plate embodiment.
Fig. 3 is the inverse layer structure figure of the utility model bioelectricity pole plate embodiment.
Referring to figs. 2 and 3, bioelectricity pole plate, comprising: bioelectrode substrate 1 is being fixed on the bioelectrode substrate just
The bioelectrode 2 in face, positioned at the electrode contact 3 of the bioelectrode substrate back side；The quantity and the life of the electrode contact 3
The quantity of object electrode 2 is identical；Each electrode contact 3 is corresponding with the position of corresponding bioelectrode 2；Each biology
Electrode 2 is connected with corresponding electrode contact by penetrating the conductive material of the bioelectrode substrate 1.
The quantity of the bioelectrode 2 and the electrode contact 3 is three；Supplemented by three difference of bioelectrodes 2
Help electrode 201, working electrode 202 and reference electrode 203.
Three electrode contacts 3 are equidistantly arranged in a row；It is located in the middle the electrode contact 3 and the work electricity
Pole 202 connects, and two electrode contacts 3 positioned at both ends are connect with the auxiliary electrode 201 and the reference electrode 203 respectively.
The shape of the working electrode 202 is circle, positioned at the center of the bioelectrode substrate 1；The auxiliary electrode
201 and the reference electrode 203 be positioned at 202 periphery of the working electrode and with the center of circle of the working electrode 202 for circle
The arc of the heart.Central angle corresponding to the auxiliary electrode 201 is greater than 180 degree, the center of circle corresponding to the reference electrode 203
Angle is less than 90 degree.
The material of the bioelectrode substrate 1 is polytetrafluoroethylene (PTFE), ceramics or glass.The shape of the bioelectrode substrate 1
Shape is round or rectangular.
The conductive material is gold, platinum gold, silver, copper, carbon nanotube or graphene.
The bioelectrode 2 is the biomaterials such as mould, antigen-antibody or nucleic acid.
The bioelectricity pole plate of the utility model the production method is as follows:
(1) bioelectrode substrate is made.Water-fast, the erosion resisting insulation material with polytetrafluoroethylene (PTFE) (PTFE), ceramics, glass etc.
For baseplate material, there is certain shapes, size and the component hole being distributed by electrode circuit contact using 3D printing technique production.Member
It is filled using conductive material in part hole.
The methods of (2) basic electrode is made: at telegraph circuit plate facade element hole position, plated using printing, sputter, change,
On circuit boards by the printing of the electrode materials such as Au, Pt, Ag, Cu, carbon nanotube, graphene, basis of formation electrode.
(3) biosensor immobilization: existed using covalent, crosslinking, absorption, self assembly, nanometer-material-modified method etc.
Immobilized biomolecule on the positive electrode of bioelectrode substrate 1 forms bioelectrode.
The utility model is a kind of dual-surface stereo formula bioelectricity pole plate (Double-Side Printed Plate), i.e., electric
It is signal circuit interface, i.e. electrode contact that pole plate, which is biological (enzyme, antigen-antibody, nucleic acid etc.) electrode, another side on one side, two sides it
Between have conductive material connection.The type electrode is convenient to connect with biological respinse analysis instrument, patches or " welding ", forms one
The strong biomolecule electronic device of kind suitability.The bioelectricity pole plate represents the developing direction of modern biotechnology microelectronics, right
Using direct bioelectrode construct high-precision bio-sensing analysis system, biosensor analysis instrument, biosensor arrays,
The fields such as the intelligent lifes systems such as bio-sensing chip, biochip, vital organ model have broad application prospects.
Fig. 4 is the structure drawing of device of the utility model bioelectronics detection device embodiment.
Fig. 5 is bioelectricity pole plate and electrode bar connection schematic diagram in the utility model bioelectricity force checking device embodiment.
Further include reaction tank 4, stir including above-mentioned bioelectricity pole plate with Fig. 5, the bioelectronics detection device referring to fig. 4
Mix device 5 and electrode bar 6；The reaction tank 4 is circular cylindrical cavity；It is provided with and the bioelectrode on 4 side wall of reaction tank
The corresponding electrode interface of the electrode contact of plate；The bioelectricity pole plate is attached on 4 side wall of reaction tank and the bioelectricity
Each electrode contact of pole plate is respectively corresponded in each electrode interface, and one end of the electrode bar 6 is provided with to be connect with each electrode
The corresponding electrode contact of mouth, each electrode contact are connected on corresponding electrode contact by the electrode interface, respectively
A electrode contact is connect with the conducting wire 10 inside the electrode bar 6, and the conducting wire 10 is connected to biological respinse analysis instrument
On；
The blender 5 is arranged in 4 bottom of reaction tank；
Liquid is additionally provided in 4 bottom of reaction tank and enters pipeline 7, is provided with liquid discharge at the top of the reaction tank 4
Mouth pipeline 8 and sample holes 9.
The electrode bar 6 is cylindrical conductive substance support stick.
The bioelectronics detection device is configured with following reagent:
Titer, buffer, cleaning solution；
It is also configured with lower component:
Reagent bottle, waste liquid bottle, sample and titer sample injector；
The biological respinse analysis instrument has power supply, circuit, panel, casing, liquid pump, and has mixing control, number
According to processing, the as the result is shown functions such as printing.
Fig. 6 be the utility model by 12 bioelectrode board groups at biologic electrode array embodiment structure chart.
Fig. 7 be the utility model by 48 bioelectrode board groups at biologic electrode array embodiment structure chart.
Multiple bioelectricity pole plates can be pressed referring to Fig. 6 and Fig. 7 according to the requirement docked with biological respinse analysis instrument
Certain sequence arranges, and prepares multi-electrode biosensor array detection device.For example 12 bioelectricity pole plates are according to the sequence of 2*6
Be arranged in 12 bioelectrode board groups at biologic electrode array, 48 bioelectricity pole plates are arranged in 48 according to the sequence of 4*8
Bioelectrode board group at biologic electrode array.
12,24,48,96 orifice plates are usually used in a variety of bioanalysis such as cell culture, the analysis of enzyme mark, biochip.This is practical
Novel bioelectricity pole plate can be combined into biologic electrode array, realize docking and detection to porous plate biological reaction pool.
Each embodiment in this specification is described in a progressive manner, the highlights of each of the examples are with other
The difference of embodiment, the same or similar parts in each embodiment may refer to each other.
Specific case used herein is expounded the principles of the present invention and embodiment, above embodiments
Explanation be merely used to help understand the method and its core concept of the utility model；Meanwhile for the general technology of this field
Personnel, based on the idea of the present invention, there will be changes in the specific implementation manner and application range.In conclusion
The content of the present specification should not be construed as a limitation of the present invention.
Claims (8)
1. a kind of bioelectricity pole plate characterized by comprising bioelectrode substrate is fixed on the bioelectrode substrate front side
Bioelectrode, positioned at the electrode contact of the bioelectrode substrate back side；The quantity of the electrode contact and the bioelectricity
The quantity of pole is identical；Each electrode contact is corresponding with the position of corresponding bioelectrode；Each bioelectrode is equal
It is connected with corresponding electrode contact by penetrating the conductive material of the bioelectrode substrate.
2. bioelectricity pole plate according to claim 1, which is characterized in that the number of the bioelectrode and the electrode contact
Amount is three；Three bioelectrodes are respectively auxiliary electrode, working electrode and reference electrode.
3. bioelectricity pole plate according to claim 2, which is characterized in that three electrode contacts are equidistantly arranged in one
Row；The electrode contact is located in the middle to connect with the working electrode, positioned at both ends two electrode contacts respectively with it is described
Auxiliary electrode is connected with the reference electrode.
4. bioelectricity pole plate according to claim 3, which is characterized in that the shape of the working electrode is circle, is located at
The center of the bioelectrode substrate；The auxiliary electrode and the reference electrode be positioned at working electrode periphery and with
The center of circle of the working electrode is the arc in the center of circle.
5. bioelectricity pole plate according to claim 1, which is characterized in that the material of the bioelectrode substrate is polytetrafluoro
Ethylene, ceramics or glass.
6. bioelectricity pole plate according to claim 1, which is characterized in that the conductive material is gold, platinum gold, silver, copper, carbon
Nanotube or graphene.
7. a kind of bioelectronics detection device, which is characterized in that including the biology as described in any one of claim 1~6
Electrode plate further includes reaction tank, blender and electrode bar；The reaction tank is circular cylindrical cavity；It is set on the reaction tank side wall
It is equipped with electrode interface corresponding with the electrode contact of the bioelectricity pole plate；The bioelectricity pole plate is attached to the reaction tank side
On wall and each electrode contact of the bioelectricity pole plate is respectively corresponded in each electrode interface, and one end of the electrode bar is arranged
There is electrode contact corresponding with each electrode interface, each electrode contact is connected to corresponding by the electrode interface
On electrode contact, each electrode contact is connect with the conducting wire inside the electrode bar, and the conducting wire is connected to biological respinse
In analysis instrument；
The blender is arranged in the reaction bottom of pond portion；
Be additionally provided with liquid in the reaction bottom of pond portion and enter pipeline, be provided at the top of the reaction tank liquid outlet conduit and
Sample holes.
8. a kind of biologic electrode array, which is characterized in that including multiple biologies as described in any one of claim 1~6
Electrode plate, multiple bioelectricity pole plates are equidistantly arranged in multiple lines and multiple rows.
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| CN201820552473.6U CN208255123U (en) | 2018-04-18 | 2018-04-18 | A kind of bioelectricity pole plate, bioelectronics detection device and biologic electrode array |
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| CN201820552473.6U CN208255123U (en) | 2018-04-18 | 2018-04-18 | A kind of bioelectricity pole plate, bioelectronics detection device and biologic electrode array |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108548857A (en) * | 2018-04-18 | 2018-09-18 | 山东省科学院生物研究所 | A kind of bioelectricity pole plate and bioelectronics detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108548857A (en) * | 2018-04-18 | 2018-09-18 | 山东省科学院生物研究所 | A kind of bioelectricity pole plate and bioelectronics detection device |
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