CN208187974U - The vitamin B12 clearance rate test macro of dialyzer - Google Patents
The vitamin B12 clearance rate test macro of dialyzer Download PDFInfo
- Publication number
- CN208187974U CN208187974U CN201820898112.7U CN201820898112U CN208187974U CN 208187974 U CN208187974 U CN 208187974U CN 201820898112 U CN201820898112 U CN 201820898112U CN 208187974 U CN208187974 U CN 208187974U
- Authority
- CN
- China
- Prior art keywords
- dialyzer
- vitamin
- visible spectrophotometer
- clearance rate
- dual wavelength
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229930003779 Vitamin B12 Natural products 0.000 title claims abstract description 31
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 title claims abstract description 31
- 239000011715 vitamin B12 Substances 0.000 title claims abstract description 31
- 235000019163 vitamin B12 Nutrition 0.000 title claims abstract description 31
- 238000012360 testing method Methods 0.000 title claims abstract description 23
- 238000010521 absorption reaction Methods 0.000 claims abstract description 30
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 230000009977 dual effect Effects 0.000 claims abstract description 18
- 238000000870 ultraviolet spectroscopy Methods 0.000 claims abstract description 17
- 238000000502 dialysis Methods 0.000 claims abstract description 15
- 239000008280 blood Substances 0.000 claims abstract description 14
- 210000004369 blood Anatomy 0.000 claims abstract description 14
- 239000002699 waste material Substances 0.000 claims abstract description 13
- 238000004088 simulation Methods 0.000 claims abstract description 9
- 239000011521 glass Substances 0.000 claims description 3
- 239000010453 quartz Substances 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000000463 material Substances 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 238000002835 absorbance Methods 0.000 description 12
- 238000000034 method Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 5
- 238000005259 measurement Methods 0.000 description 4
- 239000003053 toxin Substances 0.000 description 3
- 231100000765 toxin Toxicity 0.000 description 3
- 108700012359 toxins Proteins 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 239000011782 vitamin Substances 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- 150000003722 vitamin derivatives Chemical class 0.000 description 3
- 230000017531 blood circulation Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000002000 scavenging effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000013102 re-test Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- External Artificial Organs (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
A kind of vitamin B12 clearance rate test macro of dialyzer, it is related to dialyzer performance testing device field, including dialysis machine, simulate liquid container and waste liquid barrel, the dialyzate inlet and outlet of dialyzer are connected with dialysis machine, the blood inlet of dialyzer is connected through pump with simulation liquid container, the blood outlet of dialyzer is connected through venous chambers with waste liquid barrel, equipped with dual wavelength ultraviolet visible spectrophotometer, pipeline between venous chambers and waste liquid barrel is equipped with transparent absorption cell, the light output part of dual wavelength ultraviolet visible spectrophotometer sets up separately in the side of absorption cell, the detector of dual wavelength ultraviolet visible spectrophotometer is located at the other side of absorption cell.With measure it is simple and convenient, use manpower and material resources sparingly, test result precisely etc. beneficial effects.
Description
Technical field
The utility model relates to dialyzer performance testing device field, elaborating is that a kind of measurement is simple and convenient, saves people
Power material resources, the vitamin B12 clearance rate test macro of test result accurately dialyzer.
Background technique
It is known that generalling use the certain density toxin of configuration in vitro during loop test dialyzer performance
Simulated solution replaces blood to carry out in vitro testing, its clearance rate is calculated, as dialyzer to the index of toxin Scavenging activity.Make
For the representative species of middle molecule toxins, vitamin B12 is used to evaluate dialyzer to medium molecular substance Scavenging activity.Existing dialysis
The vitamin B12 clearance rate test system of device includes dialysis machine, simulation liquid container and waste liquid barrel, the dialyzate inlet and outlet of dialyzer with
Dialysis machine is connected, and the blood inlet of dialyzer is connected through pump with simulation liquid container, and the blood outlet of dialyzer through venous chambers and is given up
Liquid bucket is connected, and the pipeline below venous chambers is equipped with thief hatch.It is needed in test process continuously from the thief hatch below venous chambers
Collecting sample, process is cumbersome, and the sampling time is not accurate；And continuously sampled below venous chambers, it will lead to thief hatch breakage and let out
Leakage;Pressure fluctuates in pipeline liquid injecting device, and dialyzer changes therewith to vitamin B12 cleaning performance.On the other hand, dimension life
The plain light-exposed easy decomposition of B12, re-test can have certain influence to the accuracy of result after sampling.
Utility model content
The purpose of this utility model is to solve above-mentioned the deficiencies in the prior art, and it is simple and convenient to provide a kind of measurement, saves people
Power material resources, the vitamin B12 clearance rate test macro of test result accurately dialyzer.
A kind of vitamin B12 clearance rate test macro of dialyzer, including dialysis machine, simulation liquid container and waste liquid barrel, thoroughly
The dialyzate inlet and outlet of parser are connected with dialysis machine, and the blood inlet of dialyzer is connected through pump with simulation liquid container, dialyzer
Blood outlet is connected through venous chambers with waste liquid barrel, it is characterised in that is equipped with dual wavelength ultraviolet visible spectrophotometer, venous chambers
Pipeline between waste liquid barrel is equipped with transparent absorption cell, and the light output part of dual wavelength ultraviolet visible spectrophotometer sets up separately
The detector of the side of absorption cell, dual wavelength ultraviolet visible spectrophotometer is located at the other side of absorption cell.
The light output part of dual wavelength ultraviolet visible spectrophotometer described in the utility model point includes light source, monochromatic
Device one, monochromator two and chopper.It is λ 1 and λ 2 that the light that light source issues, which is separated into wavelength by monochromator one and monochromator two respectively,
Two monochromic beams (wave-length coverage be 200-380 nm), two monochromic beam λ 1 and λ 2 are replaced by chopper is incident on absorption
Pond.
The detector of dual wavelength ultraviolet visible spectrophotometer described in the utility model include photoelectric converter and
Signal processor, the photoelectric converter are photomultiplier tube.It is another that light after absorption cell absorbs is radiated at absorption cell
On the detector of side, detector detection penetrates the intensity of light, converts optical signals into electric signal, electric signal through signal processor at
Reason, the absorbance difference Δ A being converted between them calculate the concentration C i of VB12 in absorption cell.
Absorption cell described in the utility model is by quartz or glass system squarely container, surrounding uniform wall thickness one
It causes.
Absorption cell described in the utility model and the setting of dual wavelength ultraviolet visible spectrophotometer are opaque in black
Box in.Vitamin B12 will not decompose in light in detection process, and data are more accurate.
When the utility model is used, when the device used, purified water flushing pipeline and dialyzer are used first, so
" drawing blood " behaviour is carried out afterwards；Poor absorbance value is measured at this time, is denoted as A0, is the original liquid concentration in vitamin B12 simulated solution.Then into
The outer vitamin B12 clearance rate experiment of row analogue body detects the vitamin in an absorption cell at runtime at regular intervals
B12 difference absorbance A i(i=1,2,3).Poor absorbance A i is brought into the standard curve of vitamin B12, is found out corresponding
Vitamin B12 concentration C i in absorption cell.It finally carries it into clearance rate formula and calculates clearance rate.The utility model has measurement
It is simple and convenient, use manpower and material resources sparingly, test result precisely etc. beneficial effects.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model.
Specific embodiment
A kind of vitamin B12 clearance rate test macro of dialyzer, including dialysis machine 1, simulation liquid container 7 and waste liquid barrel 8,
The dialyzate inlet and outlet of dialyzer 2 are connected with dialysis machine 1, and the blood inlet of dialyzer 2 is connected through pump with simulation liquid container 7, thoroughly
The blood outlet of parser 2 is connected through venous chambers 5 with waste liquid barrel 8, and above structure is same as the prior art, repeats no more.This is practical
Novel feature is: being equipped with dual wavelength ultraviolet visible spectrophotometer, dual wavelength ultraviolet visible spectrophotometer includes light
Output par, c and detector 6, light output part point include light source 3, monochromator 1, monochromator 2 11 and chopper 10；Detector 6
Including photoelectric converter and signal processor, the photoelectric converter is photomultiplier tube.Between venous chambers 5 and waste liquid barrel 8
Pipeline be equipped with transparent absorption cell 9, absorption cell 9 be quartz or glass made of, surrounding uniform wall thickness it is consistent rectangular
Container, the light output part of dual wavelength ultraviolet visible spectrophotometer set up separately in the side of absorption cell 9, dual wavelength ultraviolet visible light
The detector 6 of spectrophotometer is located at the other side of absorption cell；The light that light source issues is respectively by monochromator 1 and monochromator 2 11
Being separated into wavelength is λ 1 and two monochromic beams of λ 2 (wave-length coverage is 200-380 nm), and two monochromic beam λ 1 and λ 2 are by cutting light
Device 10 is alternately incident on absorption cell 9, and the light after absorption cell absorbs is radiated on the detector of the absorption cell other side, detector
Detection penetrates the intensity of light, converts optical signals into electric signal, electric signal is converted between them through signal processor processes
Absorbance difference Δ A, calculates the concentration C i of VB12 in absorption cell, signal processor is according to clearance rate calculation formula and relevant information
The clearance rate of system can be calculated；Or the Ci measured is transferred to plc or other host computers by signal processor, to calculate
The clearance rate of system.
The utility model is further improved, and the absorption cell and dual wavelength ultraviolet visible spectrophotometer are arranged black
In the lighttight box of color.Vitamin B12 will not decompose in light in detection process, and data are more accurate.
When the utility model is used, purified water flushing pipeline and dialyzer are used first, then carries out " drawing blood " operation, i.e., will
Configured vitamin B12 simulated solution is full of pipeline and dialyzer.Poor absorbance value is measured at this time, is denoted as A0, A0It is vitamin
Original liquid concentration in B12 simulated solution.Then the outer vitamin B12 clearance rate experiment of analogue body is carried out, velocity of blood flow, which is respectively set, is
200/300/400ml/h, dialysate flow rate 500ml/h.At runtime, (times such as 1 or 5s or 10s at regular intervals
Interval) detection absorption cell in vitamin B12 difference absorbance A i(i=1,2,3).Each velocity of blood flow down toward
It runs 10 minutes less.Poor absorbance A i is brought into the standard curve of vitamin B12, vitamin in corresponding absorption cell is found out
The concentration C i of B12.
Wherein the standard curve of vitamin B12 obtains by the following method:
Gradient concentration is the vitamin B12 solution of 0,5,10,15,20,25,30 m mol/L, by these gradient concentrations
Vitamin B12 solution is put into absorption cell, and record absorbance value is B1, B2, B3, B4, B5, B6, B7.According to the dimension of gradient concentration
Raw element B12 solution and the poor absorbance value measured, it is mutual to draw out the corresponding poor absorbance value of vitamin B12 solution concentration
Corresponding standard curve, calculates its standard curve (form are as follows: y=ax+b), and note standard curve is Ci=Ai+ b, C in formulaiFor dimension life
The concentration of plain B12, AiFor poor absorbance, b is constant, it is desirable that the related coefficient of standard curve is more than or equal to 0.999.
It finally carries it into clearance rate formula and calculates clearance rate.
Clearance rate formula:
Clearance rate formula:
Ki: clearance rate ml/min;
C0: vitamin B12 concentration in stoste before dialysing；C0=A0+b
Ci: duct of Arantius vitamin B12 concentration after dialysis；Ci=Ai+b
QBI: blood chamber inlet flow velocity ml/min；The data of dialysis machine setting display
QF: filter out flow velocity ml/min.The data of dialysis machine setting display
The beneficial effects such as the utility model has measurement simple and convenient, uses manpower and material resources sparingly, and test result is accurate.
Claims (5)
1. a kind of vitamin B12 clearance rate test macro of dialyzer, including dialysis machine, simulation liquid container and waste liquid barrel, dialysis
The dialyzate inlet and outlet of device are connected with dialysis machine, and the blood inlet of dialyzer is connected through pump with simulation liquid container, the blood of dialyzer
Liquid outlet is connected through venous chambers with waste liquid barrel, it is characterised in that equipped with dual wavelength ultraviolet visible spectrophotometer, venous chambers and
Pipeline between waste liquid barrel is equipped with transparent absorption cell, and the light output part of dual wavelength ultraviolet visible spectrophotometer, which sets up separately, to be inhaled
The detector of the side of receives pond, dual wavelength ultraviolet visible spectrophotometer is located at the other side of absorption cell.
2. the vitamin B12 clearance rate test macro of dialyzer according to claim 1, it is characterised in that the double wave
The light output part of long ultra-violet and visible spectrophotometer point includes light source, monochromator one, monochromator two and chopper.
3. the vitamin B12 clearance rate test macro of dialyzer according to claim 2, it is characterised in that the double wave
The detector of long ultra-violet and visible spectrophotometer includes photoelectric converter and signal processor, and the photoelectric converter is light
Electric multiplier tube.
4. the vitamin B12 clearance rate test macro of dialyzer according to claim 3, it is characterised in that the absorption
Pond is consistent by quartz or glass system squarely container, surrounding uniform wall thickness.
5. the vitamin B12 clearance rate test macro of dialyzer according to claim 4, it is characterised in that the absorption
Pond and dual wavelength ultraviolet visible spectrophotometer are arranged in the lighttight box of black.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820898112.7U CN208187974U (en) | 2018-06-11 | 2018-06-11 | The vitamin B12 clearance rate test macro of dialyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820898112.7U CN208187974U (en) | 2018-06-11 | 2018-06-11 | The vitamin B12 clearance rate test macro of dialyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208187974U true CN208187974U (en) | 2018-12-04 |
Family
ID=64428166
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820898112.7U Active CN208187974U (en) | 2018-06-11 | 2018-06-11 | The vitamin B12 clearance rate test macro of dialyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208187974U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112525845A (en) * | 2020-11-12 | 2021-03-19 | 德莱福(重庆)医疗器械有限公司 | Dual-wavelength ultraviolet spectrophotometry for simultaneously testing creatinine and VB12 concentration in dialyzer clearance simulation solution |
-
2018
- 2018-06-11 CN CN201820898112.7U patent/CN208187974U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112525845A (en) * | 2020-11-12 | 2021-03-19 | 德莱福(重庆)医疗器械有限公司 | Dual-wavelength ultraviolet spectrophotometry for simultaneously testing creatinine and VB12 concentration in dialyzer clearance simulation solution |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Strathmann | Estimating the organic carbon content of phytoplankton from cell volume or plasma volume 1 | |
| CN104515857B (en) | Whole blood C reactive protein measuring method, device and sample analyser | |
| CN102519916B (en) | Method and device for on-line detecting concentration of pesticide | |
| CN102419367B (en) | Whole blood immunity measuring device and whole blood immunity measuring method | |
| CN203299129U (en) | Gravity-flow formaldehyde detection device based on microfluidic chip | |
| CN108801959A (en) | One kind being based on microflow control technique original position ammonia nitrogen on-line computing model | |
| CN104062247A (en) | Measurement device and measurement method for high-accuracy in-situ detection on pH of seawater | |
| CN101105440A (en) | Method for measuring water body total nitrogen and total phosphorous by digestion spectrophotometry of ultraviolet cooperating with ozone | |
| CN208187974U (en) | The vitamin B12 clearance rate test macro of dialyzer | |
| CN102564983A (en) | Optical detection device and method for concentration of components in turbid medium based on limited range wavelength method | |
| CN100543455C (en) | Ozone oxidation and ultraviolet scanning spectrum integral way is measured the method for total organic carbon | |
| CN107643261A (en) | A kind of monitor of long light path White pond DOAS methods measurement dusty gas concentration | |
| CN203310744U (en) | Liquid core waveguide food detector | |
| CN206906239U (en) | Water quality detection probe and water quality testing meter | |
| CN102692396B (en) | Endotoxin detection system and quantitative detection method thereof | |
| CN208537406U (en) | One kind being based on microflow control technique original position ammonia nitrogen on-line computing model | |
| CN209247626U (en) | Suitable for free chloro concentration or the on-line detector of total cl concn | |
| CN107817219A (en) | A kind of twin-stage enhanced photo acoustic spectroscopic detector device and its detection method | |
| CN103398966A (en) | Method for detecting TMC concentration in organic solution by using spectrometer | |
| CN209372694U (en) | A kind of sewage monitoring system | |
| CN206540820U (en) | A kind of chromium method UVCOD all-in-one on-line computing models | |
| CN107796800A (en) | A kind of water environment on-Line Monitor Device and method | |
| CN109238996A (en) | A kind of real-time online continuously monitors the device and its application method of nitrate concentration | |
| CN213482064U (en) | Information acquisition equipment for accurately detecting concentration of nutrient solution based on micro spectrometer | |
| CN203337551U (en) | Novel fluorescent optical fiber sensor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: No.20, Xingshan Road, Weihai Torch hi tech Industrial Development Zone, Weihai City, Shandong Province, 264210 Patentee after: Shandong Weigao blood purification products Co.,Ltd. Address before: No.20, Xingshan Road, torch hi tech Industrial Development Zone, Huancui District, Weihai City, Shandong Province 264200 Patentee before: WEIHAI WEIGAO BLOOD PURIFICATION PRODUCTS Co.,Ltd. |