CN207862353U - A kind of Tissue Culture Flask of attached cell and suspension cell co-cultivation - Google Patents
A kind of Tissue Culture Flask of attached cell and suspension cell co-cultivation Download PDFInfo
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- CN207862353U CN207862353U CN201820101135.0U CN201820101135U CN207862353U CN 207862353 U CN207862353 U CN 207862353U CN 201820101135 U CN201820101135 U CN 201820101135U CN 207862353 U CN207862353 U CN 207862353U
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Abstract
The utility model discloses the Tissue Culture Flasks that a kind of attached cell and suspension cell co-culture, it is equipped with bottleneck, bottle cap including body, body, nanometer semi-permeable membrane is equipped in the body, space in body is divided into two parts by nanometer semi-permeable membrane, the culture vessel of attached cell, the culture vessel of suspension cell are served as, bottle cap is gas permeability bottle cap.The cell factor that two kinds of cell manipulation phases of the technical program are independent of each other, but are secreted between two kinds of cells can pass through the semi-transparent membrane interaction of nanometer, the in a sense interaction and influence between two kinds of cells of actual response.
Description
Technical field
The utility model is related in Bioexperiment consumptive material field, be exactly that a kind of attached cell and suspension cell are trained altogether
Foster Tissue Culture Flask.
Background technology
Tissue Culture Flask, as the term suggests it is exactly to be used for cultivating cell.But the classification one of all cultures currently on the market
As can be divided into according to material glass and plastics, bottom shape has square, rectangle, triangle etc., is held according to bottle
Product has 5 to 500ml selective.In addition, surface after special modification, can allow cell preferably to carry out adhere-wall culture,
25~175cm can be divided into according to the adherent area of cell again in this way2It is a variety of.But two kinds of different Cultural types it are not carried out at the same time
（Attached cell and suspension cell）The cell bottle of co-incubation.
Semi-permeable membrane is a kind of film passed in and out to certain molecule or ion diffusion, to different particles by having selection
The film of property.Such as cell membrane, pericystium, parchment and the collodion film etc. manually made.Semi-permeable membrane for permeate colloidal sol and
Measure osmotic pressure etc..It is also to be carried out by semi-permeable membrane that biology, which draws nutrient,.Semi-permeable membrane, which is a kind of, allows ion and small point
The freely through membrane structure of son, large biological molecule cannot pass freely through semi-permeable membrane, the reason is that the size ratio of the hole of semi-permeable membrane
Ion and small molecule are big but smaller than large biological molecule such as protein and starch.Semi-permeable membrane only allows solution to pass through in chemistry, glue
Body and turbid cannot pass through.The aperture of different types of semi-permeable membrane is different.Biomembrane is exactly a kind of semi-permeable membrane, and plant is thin
The plasm layer of born of the same parents collectively constitutes an osmosis system with cell liquid, can allow for some small molecules such as water, oxygen, dioxy
Change carbon etc. to pass freely through.
Cytology research always tends to simulation human body environment and removes culture cell at present, it is intended to find out crucial in cell culture
Intercellular interaction mechanism, but the limitation of experimental tool, frequently resulting in experiment can not carry out or failure, be not achieved pre-
Phase effect cannot judge the correctness of experimental considerations.Such as it studies：Study blood in cell how with the cell on blood vessel
It influences each other.So how to invent a kind of attached cell and Tissue Culture Flask that suspension cell co-cultures is asked at urgently to be resolved hurrily
Topic.
Current Tissue Culture Flask can not all accomplish the cells of two kinds of different Cultural types co-incubation together, due to not having
There are suitable simulated environment container in vivo or cell to co-culture bottle, frequently resulting in the co-culture experiments of two kinds of cells can not carry out,
Many cytology research experimental designs and Research approaches are caused to terminate or go for a stroll.
Existing substitute technology has following two at present, but has serious insufficient or defect：
1, the first alternative solution：The culture medium of one of which cell culture is used to be added to as cell culture additive
In the cell culture medium of two kinds of cells, but this method cannot be permanent allow second of cytositimulation culture, because concentrating
When the first cell culture medium, the variation of content and concentration is may result in, if being continuously added into the concentration training of the first cell
Base is supported, the various culture indexs of the cell culture medium of second of cell can be destroyed, and cause experiment that can not carry out.
2, second of alternative solution：By force by two distinct types of cell co-culture, actual conditions are two kinds of growths
Different types of cell is cultivated in the same Tissue Culture Flask, when variety classes cell is cultivated in space can because
It is smaller that suspension cell settlement action covering attached cell causes attached cell to be contacted with culture medium, and then leads to adherent cell growth
It is relatively slow or even dead, while two kinds of cells of more difficult sorting, there is very big obstruction to follow-up test or leads to experimental error；Two kinds simultaneously
Whether the cell culture medium of cell is identical, if can use with will also treat as the case may be.
Utility model content
The technical problem to be solved by the present invention is to provide the cell trainings that a kind of attached cell and suspension cell co-culture
Bottle is supported, the cell factor which can allow two kinds of cells to be secreted can carry out infiltration interaction, actual response by semi-permeable membrane
The interaction of two kinds of cells, can solve the problems, such as well attached cell and suspension cell cannot co-incubation.
In order to solve the above technical problems, the utility model uses following technological means：
A kind of Tissue Culture Flask of attached cell and suspension cell co-cultivation, including body, body are equipped with bottleneck, bottle cap,
Nanometer semi-permeable membrane is equipped in the body, the space in body is divided into two parts, serves as patch by nanometer semi-permeable membrane
The culture vessel of parietal cell, the culture vessel of suspension cell, bottle cap are gas permeability bottle cap.
The technical program, it is intermediate on the basis of traditional Tissue Culture Flask to have added one layer of nanometer semi-permeable membrane, nanometer semi-transparent
The molecule of film can be set as needed through aperture, specifically, according to interactional outer secretion factor between the cell of research
It is of different sizes, the nanometer semi-permeable membrane of different pore size is designed, the culture between different cells is met.The cell co-cultures bottle bottle simultaneously
Mouthful changing liquid demand also according to liquid feeding becomes be combined two semicircle bottlenecks, convenient for isolation culture space and separate liquid feeding with
Change liquid.The cell factor that two kinds of cell manipulation phases of the technical program are independent of each other, but are secreted between two kinds of cells can be with
Pass through the semi-transparent membrane interaction of nanometer, the in a sense interaction and influence between two kinds of cells of actual response.
Co-cultured cell culture bottle process for using：
Culture bottle cap is opened, takes the resuspension cell culture fluid containing attached cell of certain volume that body is added with pipette
Nanometer semi-permeable membrane side a part, culture volume, which is settled at nanometer semi-permeable membrane, is impregnated with a nanometer semi-permeable membrane；It uses again another
Another part of body is added in the cell culture fluid that a piece pipette takes certain volume to contain suspension cell, then places titanium dioxide
Carbon culture is cultivated.When passage cell, first by suspension cell mixing, then cell suspension is shifted with pipette
Into centrifuge tube, then plus culture medium rinses twice, then flushing liquor is transferred in centrifuge tube；Secondly nanometer semi-permeable membrane is another
The cell culture fluid suction of side is abandoned, and gently rinses bottom twice with physiological saline, cell dissociation is got off according to cell dissociation flow
And it is transferred in centrifuge tube and waits for that lower step experiment is used.
Further optimal technical scheme is as follows：
The nanometer semi-permeable membrane is 0.3-1cm at a distance from the bottle wall of wherein side.Range is two kinds thin in 0.3-1cm
What born of the same parents operated the phase cell factor that is independent of each other, but is secreted between two kinds of cells can be by the semi-transparent membrane interaction of nanometer.
The center of the bottle cap is equipped with ventilation mouth, and ventilation mouth fills gas permeable material.The cell co-cultures bottle body whole body
For polystyrene material, bottleneck designs for torticollis.
The advantageous effect of the technical program：
1, two distinct types of cell can be made to be co-cultured, infiltration interaction can be carried out by nanometer semi-permeable membrane,
The interaction of two kinds of cells of actual response, can solve well attached cell and suspension cell cannot co-incubation this is difficult
Topic, to reach experiment effect.
2, in the same relatively independent space, cell culture time, consumptive material are saved, cell is improved and co-cultures phase interaction
With efficiency, the success rate of experiment is improved.
3, two kinds of cells reduce open-sky technique number in the same culture bottle culture, greatly reduce internal and external
Cross contamination probability.
Description of the drawings
Fig. 1 is the structural schematic diagram of the utility model.
Fig. 2 is the vertical view of Fig. 1.
Reference sign：1- bottle caps；2- bottlenecks；3- bodies；The culture vessel of 4- attached cells；5- nanometers of semi-permeable membranes；
The culture vessel of 6- suspension cells.
Specific implementation mode
With reference to embodiment, the utility model is further illustrated.
Referring to Fig. 1, Fig. 2 it is found that the Tissue Culture Flask that a kind of attached cell of the utility model and suspension cell co-culture,
It is made of body 3, bottleneck 2 and bottle cap 1；Body 3 is equipped with bottleneck 2, and nanometer semi-permeable membrane 5 is equipped in body 3, and nanometer semi-permeable membrane 5 will
Space in body 3 is divided into two parts, serves as the culture vessel 4 of attached cell, the culture vessel 6 of suspension cell,
Bottle cap 1 is gas permeability bottle cap 1.
The technical program, intermediate on the basis of traditional Tissue Culture Flask to have added one layer of nanometer semi-permeable membrane 5, nanometer is semi-transparent
The molecule of film 5 can be set as needed through aperture, specifically, according to interactional outer secretion factor between the cell of research
It is of different sizes, the nanometer semi-permeable membrane 5 of different pore size is designed, the culture between different cells is met.The cell co-cultures bottle simultaneously
Bottleneck 2 changes liquid demand also according to liquid feeding and becomes be combined two semicircle bottlenecks 2, convenient for completely cutting off culture space and separately adding
Liquid and change liquid.The cell factor that two kinds of cell manipulation phases of the technical program are independent of each other, but are secreted between two kinds of cells
It can be interacted by nanometer semi-permeable membrane 5, in a sense the interaction and influence between two kinds of cells of actual response.
The nanometer semi-permeable membrane 5 is 0.3-1cm at a distance from the bottle wall of wherein side.Range is two kinds thin in 0.3-1cm
Born of the same parents operate can interacting by nanometer semi-permeable membrane 5 for the cell factor that phase is independent of each other, but is secreted between two kinds of cells.
The center of the bottle cap 1 is equipped with ventilation mouth, and ventilation mouth fills gas permeable material.It is complete that the cell co-cultures bottle body 3
As polystyrene material, bottleneck 2 designs for torticollis.
Co-cultured cell culture bottle process for using：
Culture bottle cap 1 is opened, takes the resuspension cell culture fluid containing attached cell of certain volume that bottle is added with pipette
One part of 5 side of nanometer semi-permeable membrane of body 3, culture volume, which is settled at nanometer semi-permeable membrane 5, is impregnated with nanometer semi-permeable membrane 5；
Another part of body 3 is added in the cell culture fluid for taking certain volume to contain suspension cell with another pipette again, then puts
Carbon dioxide culture is set to be cultivated.When passage cell, first by suspension cell mixing, then with pipette by cell
Suspension is transferred in centrifuge tube, and then plus culture medium rinses twice, then flushing liquor is transferred in centrifuge tube；Secondly by nanometer half
The cell culture fluid suction of 5 other side of permeable membrane is abandoned, and bottom is gently rinsed twice with physiological saline, according to cell dissociation flow cell
It digests and is transferred in centrifuge tube and wait for that lower step experiment is used.
The advantageous effect of the technical program：
1, two distinct types of cell can be made to be co-cultured, infiltration interaction can be carried out by nanometer semi-permeable membrane 5,
The interaction of two kinds of cells of actual response, can solve well attached cell and suspension cell cannot co-incubation this is difficult
Topic, to reach experiment effect.
2, in the same relatively independent space, cell culture time, consumptive material are saved, cell is improved and co-cultures phase interaction
With efficiency, the success rate of experiment is improved.
3, two kinds of cells reduce open-sky technique number in the same culture bottle culture, greatly reduce internal and external
Cross contamination probability.
Since the foregoing is merely specific embodiment of the present utility model, but the protection of the utility model is without being limited thereto,
Any those skilled in the art it is contemplated that the equivalent variation or replacement of the technical program technical characteristic, all covers
Within the scope of protection of the utility model.
Claims (3)
1. the Tissue Culture Flask that a kind of attached cell and suspension cell co-culture, including body, body are equipped with bottleneck, bottle cap,
It is characterized in that：Nanometer semi-permeable membrane is equipped in the body, the space in body is divided into two parts by nanometer semi-permeable membrane, respectively
It is used as the culture vessel of attached cell, the culture vessel of suspension cell, bottle cap is gas permeability bottle cap.
2. the Tissue Culture Flask that attached cell according to claim 1 and suspension cell co-culture, it is characterised in that：It is described
Nanometer semi-permeable membrane at a distance from the bottle wall of wherein side be 0.3-1cm.
3. the Tissue Culture Flask that attached cell according to claim 1 and suspension cell co-culture, it is characterised in that：It is described
Bottle cap center be equipped with ventilation mouth, ventilation mouth fill gas permeable material.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820101135.0U CN207862353U (en) | 2018-01-22 | 2018-01-22 | A kind of Tissue Culture Flask of attached cell and suspension cell co-cultivation |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201820101135.0U CN207862353U (en) | 2018-01-22 | 2018-01-22 | A kind of Tissue Culture Flask of attached cell and suspension cell co-cultivation |
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| CN207862353U true CN207862353U (en) | 2018-09-14 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107937271A (en) * | 2018-01-22 | 2018-04-20 | 湖北省银丰鼎诚生物工程有限公司 | The Tissue Culture Flask that a kind of attached cell and suspension cell co-culture |
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2018
- 2018-01-22 CN CN201820101135.0U patent/CN207862353U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107937271A (en) * | 2018-01-22 | 2018-04-20 | 湖北省银丰鼎诚生物工程有限公司 | The Tissue Culture Flask that a kind of attached cell and suspension cell co-culture |
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