CN207793283U - Constant pressure cell pressurization culture apparatus - Google Patents
Constant pressure cell pressurization culture apparatus Download PDFInfo
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- CN207793283U CN207793283U CN201721739065.3U CN201721739065U CN207793283U CN 207793283 U CN207793283 U CN 207793283U CN 201721739065 U CN201721739065 U CN 201721739065U CN 207793283 U CN207793283 U CN 207793283U
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- pressure
- cell
- constant pressure
- culture apparatus
- pressurizes
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Abstract
A kind of constant pressure cell pressurization culture apparatus, for being cultivated the cell in Tissue Culture Dish under stable static pressure, which is characterized in that including：Cell incubator, for placing Tissue Culture Dish, top have for Tissue Culture Dish by opening, be provided with charging valve and drain tap, barometer, for measuring in real time and showing the air pressure in cell incubator on babinet；And sealing constant pressure unit, opening is sealed mounted on opening and adjusts the air pressure in cell incubator, wherein, sealing constant pressure unit has the piston component for being mounted on top open part and vertical pressure-applying unit, piston component has and the pressurizing piston that can be slided up and down that matches that is open, vertical pressure-applying unit setting is adding top land, and for providing the gas in the cell incubator of pressure compression straight down, vertical pressure-applying unit includes several counterweights and regulating tank.
Description
Technical field
The utility model is related to a kind of cell culture apparatus, it is specifically related to carry out cell culture under a kind of constant-pressure conditions
Device.
Background technology
The biological study of cell at various pressures has the research of union, high blood pressure and glaucoma-controlling etc.
It is significant.Current common cell pressuring method has fluid pressure type pressurization and air pressure pressurization.Hydraulic pressurization is directly to cell
Culture solution pressurizes, although the control of such pressuring method pressure and temperature is relatively stable, it is dense cannot to simulate different oxygen
The influence that degree or gas constituent react cell biology.Air pressure pressurization be by being filled with high pressure gas to closed container,
Increase the pressure of cell culture fluid indirectly and then pressurizes to cell.Air pressure pressurization is relative to the advantages of hydraulic pressurization being capable of mould
The biological impact that quasi- high pressure oxygen environment is proliferated cell metabolism with the gaseous environment for being mixed with hydrogen or other gases.
However conventional air pressure pressurization incubator makes to maintain pressure in incubator to need the high pressure gas being persistently filled with
In incubator gas be always flowing, be unfavorable for it is continual and steady pressurize to cell, and the constant flow of gas
It can lead to the non-uniform temperature in incubator, change greatly, temperature uniformity is poor.
Utility model content
The utility model is to lead to and incubator unstable to cell pressurization to solve the above-mentioned high pressure gas being persistently filled with
The problem of interior temperature uniformity difference is, and it is an object of the present invention to provide a kind of constant pressure cell culture apparatus.
Constant pressure cell pressurization culture apparatus provided by the utility model, is used under stable static pressure to cell culture
Cell in ware is cultivated, which is characterized in that including：Cell incubator, for placing Tissue Culture Dish, top has for thin
Born of the same parents' culture dish by opening, charging valve and drain tap are provided on babinet, barometer is thin for measuring and showing in real time
Air pressure in born of the same parents' incubator；And sealing constant pressure unit, it seals opening mounted on opening and adjusts the gas in cell incubator
Pressure, wherein sealing constant pressure unit has piston component and vertical pressure-applying unit mounted on top open part, piston component tool
Have and match the pressurizing piston that can be slided up and down with opening, vertical pressure-applying unit setting is adding top land, perpendicular for providing
Gas in straight downward pressure compression cell incubator, vertical pressure-applying unit includes several counterweights and regulating tank.
Constant pressure cell pressurization culture apparatus provided by the utility model, can also have the feature that, it is characterised in that：
Wherein, drain tap is arranged on the peripheral wall of babinet, and charging valve is also disposed on the peripheral wall of babinet and under drain tap
Side.
Constant pressure cell pressurization culture apparatus provided by the utility model, can also have the feature that, it is characterised in that：
Wherein, charging valve is triple valve.
Constant pressure cell pressurization culture apparatus provided by the utility model, can also have the feature that, it is characterised in that：
Wherein, pressurizing piston component further includes sealing ring, and sealing ring is socketed in pressurizing piston, is trained for sealing pressing piston and cell
Support the gap between case.
Constant pressure cell pressurization culture apparatus provided by the utility model, can also have the feature that, it is characterised in that：
Wherein, there are several grooves, the groove is for placing vertical pressure-applying unit at the top of pressurizing piston.
Constant pressure cell pressurization culture apparatus provided by the utility model, can also have the feature that, it is characterised in that：
Wherein, several counterweights have different quality, and the quality of counterweight is 5-25kg.
Constant pressure cell pressurization culture apparatus provided by the utility model, can also have the feature that, it is characterised in that：
Wherein, there is regulating tank closed water tank, the top of water tank to have inlet valve, have outlet valve on peripheral wall.
Constant pressure cell pressurization culture apparatus provided by the utility model, can also have the feature that, it is characterised in that：
Wherein, also there is vertical pressure-applying unit constant flow pump, the input terminal of the constant flow pump to be connected to regulating tank, for water in regulating tank
The adjusting of amount.
Constant pressure cell pressurization culture apparatus provided by the utility model, can also have the feature that, it is characterised in that：
Wherein, constant pressure cell culture apparatus also has limiting plate, which is fixed on the box house of cell incubator, for preventing
Pressurizing piston slides, limiting plate have for Tissue Culture Dish by through-hole, the width of the through-hole is less than the width of pressurizing piston
Degree.The effect of utility model
The utility model is related to constant pressure cell pressurization culture apparatus in cell incubator by sealing constant pressure unit seal,
So that fixed air pressure can be maintained in cell incubator to carry out cell culture, avoids and come using the high pressure gas of flowing
Air pressure is maintained, is solved well due to cell pressurization shakiness caused by gas constant flow and cell incubator temperature uniformity difference
Problem.
Constant pressure cell pressurization culture apparatus in the utility model also has can be in the opening of cell culture box top
The pressurizing piston of lower slider and for providing the vertical pressure-applying unit of gas in the compression cell incubator of pressure straight down.When thin
When pressure in born of the same parents' incubator is lost, downward pressure-driven pressurizing piston that vertical pressure-applying unit can be provided timely
It moves downward, reduces the gas volume in cell incubator, and then supplement the pressure of loss so that tieed up always in cell incubator
Hold scheduled air pressure.
Description of the drawings
Fig. 1 is the structural schematic diagram of constant pressure cell pressurization culture apparatus；And
Fig. 2 is the structural schematic diagram of regulating tank.
Specific implementation mode
In order to make the technical means, creative features, achievement of purpose, and effectiveness of the utility model be easy to understand, with
Lower embodiment combination attached drawing pressurizes the composition of culture apparatus, operation principle and beneficial to constant pressure cell provided by the utility model
Effect is specifically addressed.
Fig. 1 is the structural schematic diagram of constant pressure cell pressurization culture apparatus.
As shown in Figure 1, in the present embodiment constant pressure cell pressurization culture apparatus 1 include cell incubator 11, barometer 12,
Seal constant pressure unit 13 and limiting plate 14.
Cell incubator 11 has cylindrical box, charging valve 111 and air bleeding valve for placing Tissue Culture Dish A
Door 112.Top of the box have for Tissue Culture Dish A by circular open, 111 He of drain tap is installed on the peripheral wall of babinet
Charging valve 112, drain tap 111 are mounted in upper the 1/3 of peripheral wall, and charging valve 112 is located in lower the 1/3 of peripheral wall.Inflation
Valve 112 is three-way control valve, for being passed through different types of gas.
Barometer 12 is, test side be arranged inside cell incubator 11, display end is arranged in cell incubator 11
Outside babinet, for detecting in real time and showing the atmospheric pressure value inside cell incubator 11.
It seals constant pressure unit 13 to be mounted at the circular open of cell incubator top of the box, for sealing circular open simultaneously
Adjust the air pressure in cell incubator, including pressurizing piston 131, sealing ring 132 and vertical pressure-applying unit.
Pressurizing piston 131 is cylinder, is mounted in circular open, and outer diameter and the diameter of circular open phase match,
It can be slided up and down in circular open.The top of pressurizing piston 131 has downward groove, for placing vertical pressurization group
Part.Sealing ring 132 is socketed on 131 outer wall of pressurizing piston, for the gap between sealing pressing piston 131 and circular open.It is close
Seal 132 is sealing ring made of medical silica-gel.
Fig. 2 is the structural schematic diagram of regulating tank.
As shown in Fig. 2, vertical pressure-applying unit includes five different counterweights of quality 133, regulating tank 134 and constant flow pump
135.The weight of five counterweights 133 is respectively 5kg, 10kg, 15kg, 20kg and 25kg.Regulating tank 134 has closed water
Babinet, the top of water tank have an inlet valve 1341, peripheral wall lower 1/3 in outlet valve 1342 is installed, outlet valve 1342 with it is permanent
The input terminal connection of stream pump 135.Constant flow pump 135 can accurately control the water being discharged in regulating tank 134 so that regulating tank
134 weight can be finely adjusted, and then the air pressure in cell incubator 11 is adjusted.
Limiting plate 14 is weldingly fixed on the inner wall of cylindrical box, for preventing pressurizing piston from sliding.Limiting plate 14 is
Circle, diameter are equal with the internal diameter of cylindrical box.On limiting plate 14 have for Tissue Culture Dish A by circular through hole, circle
The center of circle of shape through-hole is overlapped with the center of circle of limiting plate 14, and the diameter of circular through hole is less than the outer diameter of pressurizing piston 131.
It elaborates below to the operational process and principle of constant pressure cell provided in this embodiment pressurization culture apparatus.
When needing to study biology translation of the cell under the effect of a certain constant pressure, Tissue Culture Dish A is put into carefully
In born of the same parents' incubator 11, it is inserted into pressurizing piston 131 and sealing ring 132 seals cell incubator 11.Drain tap 111 is fastened, is led to
The air that air valve 112 is filled with certain volume (Vml) is overcharged, observes the reading (P) of barometer 12 when barometer shows cell training
The air pressure supported in case 11 is filled with gas close to stopping when subscribing range, closes charging valve 112.Counterweight 133 is placed on pressurization
The recess at 131 top of piston, pressure in incubator is adjusted by adjusting load two (M) of counterweight 133.Again to regulating tank
Water filling in 134, and regulating tank 134 is also placed to the recess at 131 top of pressurizing piston, constant flow pump 135 is opened according to air pressure
The reading of meter 12 adjusts the water in regulating tank, and extra water is expelled to and obtains scheduled pressure.Again by cell incubator
11 are positioned over progress cell culture in the cultivation temperature of setting.
When how slight change causes the pressure in cell incubator 11 to fluctuate to ambient temperature, by adjusting counterweight
133 and regulating tank 134 can adjust the air pressure in cell incubator 11 in a certain range, ensure 11 internal pressure of cell incubator
By force within a predetermined range, to realize the persistent pressure to cell in Tissue Culture Dish A.
The effect of embodiment
The utility model is related to constant pressure cell pressurization culture apparatus in cell incubator by sealing constant pressure unit seal,
So that fixed air pressure can be maintained in cell incubator to carry out cell culture, avoids and come using the high pressure gas of flowing
Air pressure is maintained, is solved well due to cell pressurization shakiness caused by gas constant flow and cell incubator temperature uniformity difference
Problem.
Constant pressure cell pressurization culture apparatus in the utility model also has can be in the opening of cell culture box top
The pressurizing piston of lower slider and for providing the vertical pressure-applying unit of gas in the compression cell incubator of pressure straight down.When thin
When pressure in born of the same parents' incubator is lost, downward pressure-driven pressurizing piston that vertical pressure-applying unit can be provided timely
It moves downward, reduces the gas volume in cell incubator, and then supplement the pressure of loss so that tieed up always in cell incubator
Hold scheduled air pressure.
Further, since charging valve is three-way valve, the gas group for being filled with cell incubator can easily be adjusted
Point and ratio, to study biological impact of the gas to cell of different constituents.
The water in regulating tank is adjusted using constant flow pump in the utility model, it can be accurately according to pressure gauge
Subtle adjusting is carried out to the air pressure in cell incubator.Make the adjusting of pressure since the drainage speed of constant flow pump is relatively slow
More accurate careful, being not easy to adjust excessively leads to the failure of an experiment.
This test it is novel in limiting plate for prevent experiment midway equipment break down when pressurizing piston slide to bottom
Tissue Culture Dish in breaking test, leads to the failure of an experiment.
The above embodiment is the preferred case of the utility model, is not intended to limit the scope of protection of the utility model.
Claims (9)
- The culture apparatus 1. a kind of constant pressure cell pressurizes, for being carried out to the cell in Tissue Culture Dish under stable static pressure Culture, which is characterized in that including：Cell incubator, for placing Tissue Culture Dish, top have for the Tissue Culture Dish by opening, set on babinet It is equipped with charging valve and drain tap；Barometer, for measuring in real time and showing the air pressure in the cell incubator；AndConstant pressure unit is sealed, the opening is sealed mounted on the opening and adjusts the air pressure in the cell incubator,Wherein, the sealing constant pressure unit has piston component and vertical pressure-applying unit mounted on the top open part,The piston component has to match the pressurizing piston that can be slided up and down with the opening,The vertical pressure-applying unit is arranged at the top of the pressurizing piston, compresses the cell training for providing pressure straight down The gas in case is supported,The vertical pressure-applying unit includes several counterweights and regulating tank.
- The culture apparatus 2. constant pressure cell according to claim 1 pressurizes, it is characterised in that：Wherein, the drain tap is arranged on the peripheral wall of the babinet,The charging valve is also disposed on the peripheral wall of the babinet and below the drain tap.
- The culture apparatus 3. constant pressure cell according to claim 1 pressurizes, it is characterised in that：Wherein, the charging valve is triple valve.
- The culture apparatus 4. constant pressure cell according to claim 1 pressurizes, it is characterised in that：Wherein, the pressurizing piston component further includes sealing ring,The sealing ring is socketed in the pressurizing piston, for sealing the seam between the pressurizing piston and the cell incubator Gap.
- The culture apparatus 5. constant pressure cell according to claim 1 pressurizes, it is characterised in that：Wherein, there are several grooves, the groove is for placing the vertical pressure-applying unit at the top of the pressurizing piston.
- The culture apparatus 6. constant pressure cell according to claim 1 pressurizes, it is characterised in that：Wherein, several described counterweights have different quality,The quality of the counterweight is 5-25kg.
- The culture apparatus 7. constant pressure cell according to claim 1 pressurizes, it is characterised in that：Wherein, there is the regulating tank closed water tank, the top of the water tank to have inlet valve, have on peripheral wall Water valve.
- The culture apparatus 8. constant pressure cell according to claim 7 pressurizes, it is characterised in that：Wherein, also there is the vertical pressure-applying unit constant flow pump, the input terminal of the constant flow pump to be connected to, be used for the regulating tank The adjusting of water in the regulating tank.
- The culture apparatus 9. constant pressure cell according to claim 1 pressurizes, it is characterised in that：Wherein, also there is the constant pressure cell culture apparatus limiting plate, the limiting plate to be fixed on the babinet of the cell incubator Inside, for preventing the pressurizing piston from sliding,The limiting plate have for the Tissue Culture Dish by through-hole, the width of the through-hole is less than the pressurizing piston Width.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721739065.3U CN207793283U (en) | 2017-12-13 | 2017-12-13 | Constant pressure cell pressurization culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721739065.3U CN207793283U (en) | 2017-12-13 | 2017-12-13 | Constant pressure cell pressurization culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207793283U true CN207793283U (en) | 2018-08-31 |
Family
ID=63284075
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721739065.3U Expired - Fee Related CN207793283U (en) | 2017-12-13 | 2017-12-13 | Constant pressure cell pressurization culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207793283U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110511871A (en) * | 2019-09-25 | 2019-11-29 | 西北农林科技大学 | A kind of co-culture method for the device and ox myocyte and fat cell that cell co-cultures |
| WO2022012607A1 (en) * | 2020-07-17 | 2022-01-20 | 上海我武干细胞科技有限公司 | Incubator |
| CN114015545A (en) * | 2021-11-04 | 2022-02-08 | 方国平 | Novel anti-pollution constant-pressure culture device for expression vector |
-
2017
- 2017-12-13 CN CN201721739065.3U patent/CN207793283U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110511871A (en) * | 2019-09-25 | 2019-11-29 | 西北农林科技大学 | A kind of co-culture method for the device and ox myocyte and fat cell that cell co-cultures |
| WO2022012607A1 (en) * | 2020-07-17 | 2022-01-20 | 上海我武干细胞科技有限公司 | Incubator |
| CN115720592A (en) * | 2020-07-17 | 2023-02-28 | 上海我武干细胞科技有限公司 | Culture box |
| JP2023510026A (en) * | 2020-07-17 | 2023-03-10 | 上海我武幹細胞科技有限公司 | incubator |
| JP7368633B2 (en) | 2020-07-17 | 2023-10-24 | 上海我武幹細胞科技有限公司 | incubator |
| CN114015545A (en) * | 2021-11-04 | 2022-02-08 | 方国平 | Novel anti-pollution constant-pressure culture device for expression vector |
| CN114015545B (en) * | 2021-11-04 | 2024-04-19 | 浙江康泽生物技术有限公司 | Anti-pollution constant pressure type culture device for expression vector |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180831 Termination date: 20181213 |