CN207713497U - A kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria - Google Patents

A kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria Download PDF

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Publication number
CN207713497U
CN207713497U CN201721805051.7U CN201721805051U CN207713497U CN 207713497 U CN207713497 U CN 207713497U CN 201721805051 U CN201721805051 U CN 201721805051U CN 207713497 U CN207713497 U CN 207713497U
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China
Prior art keywords
liquid level
reactor body
oxidizing bacteria
ammonia oxidizing
enrichment culture
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Expired - Fee Related
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CN201721805051.7U
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Chinese (zh)
Inventor
张帆
兰兰
王晓慧
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Changan University
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Changan University
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  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The utility model discloses a kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria,The present apparatus on the ontology cover board of reactor body upper end by being arranged water sealing (sealing water) pipe and gas gathering mask,The recycling and its recycling to nitrogen are realized simultaneously,Embody economical and practical principle,And coordinate the multiplication characteristic and its enrichment culture mode of anaerobic ammonia oxidizing bacteria,It devises a full set of automatic control device and avoids the problem of not agreeing with,Because SBR can be divided into it is aerobic,The methods of operation such as anaerobism,The present apparatus removes the oxygen in reactor to build an anaerobic environment by exposing nitrogen into reactor,When exposing oxygen or other various gases for needing to be passed through reactor into reactor,The device is also used for other various anaerobism except anaerobic ammonia oxidizing bacteria,The enrichment culture of aerobic bacteria,The function of collecting the gas generated in reaction is had both simultaneously,The gas of generation is carried out so as to further qualitative,Quantitative analysis.

Description

A kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria
Technical field
The utility model belongs to technical field of sewage, and in particular to a kind of enrichment culture gas collection of anaerobic ammonia oxidizing bacteria Device.
Background technology
Currently, Anammox (anaerobic ammonium oxidation, ANAMMOX), which is one kind, having efficiently warp The denitrification process of Ji, anaerobic ammonia oxidizing bacteria (AOB) utilize NH4 +For electron donor, NO2 -For electron acceptor, reaction generates nitrogen, To realize denitrogenation of waste water.Meanwhile SBR (Sequencing Batch Reactor Activated Sludge Process, Sequencing batch reactor) it is a kind of activated sludge sewage disposal technology of intermittent mode operation, the method for operation is various, It is chiefly used in the culture and sewage disposal of bacterium.Advantageous SBR needs more to suit anaerobism ammonia oxygen in starting Anammox The improvement of change.In existing SBR starts Anammox technology, the traditional SBR of the required anaerobic environments of AOB can not be very Good offer, while Anammox this bioprocess will produce nitrogen and other possible intermediate gas, traditional SBR The demand that researcher studies the gas generated in reaction cannot be met, therefore advantageous in starting Anammox SBR needs the improvement for more suiting Anammox.
Utility model content
It is existing to overcome the purpose of this utility model is to provide a kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria The deficiency of technology.
In order to achieve the above objectives, the utility model adopts the following technical solution:
A kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria, including control device, reactor body and for anti- The babinet of device ontology heating water bath, reactor body upper end is answered to be equipped with ontology cover board, bottom is equipped with aeration sand in reactor body Disk, ontology cover board are equipped with water sealing (sealing water) pipe and gas gathering mask, and gas gathering mask is connected with plenum chamber by conveyance conduit, and aeration sand table passes through gas Pipe is connected with nitrogen cylinder, and aeration sand table is connected to plenum chamber by tracheae, and liquid level measurer, stirring are additionally provided in reactor body Device, temperature probe, liquid level measurer are connected to liquid level relay, and temperature probe is connected to temperature controller, liquid level relay, stirring Device and temperature controller are all connected to control device.
Further, control device includes control cabinet, and control cabinet is equipped with time switch, attaching plug, liquid level relay And master switch, reactor body upper end are equipped with lug, lug is equipped with through-hole, and reactor body is connected with ontology cover board by bolt It connects.
Further, gas gathering mask by reactor body to outside be tapered mouth;Water is equipped between water sealing (sealing water) pipe and ontology cover board Tube sealing set.
Further, upper end is equipped with buckle in babinet, is stamped cover board on the buckle of babinet, heating rod is fixed on cover board.
Further, peristaltic pump is connected with by water inlet pipe in reactor body, reactor body is interior defeated by peristaltic pump Nutrient needed for anaerobic ammonia oxidizing bacteria is sent, reactor body is interior to be connected with immersible pump by outlet pipe, and outlet pipe is placed in reactor sheet Internal lower end.
Further, agitating device includes the stirring-head being placed in reactor body and the machine that is placed on the outside of reactor body Tool blender, mechanical agitator are stirring motor or stirring motor;Mechanical agitator passes through gear drive with stirring.
Further, liquid level measurer includes the low liquid level electric wire being set in reactor body and high liquid level electric wire, low Liquid level electric wire is less than high liquid level electric wire.
Further, the tracheae that aeration sand table is connect with plenum chamber and nitrogen cylinder is equipped with triple valve, triple valve to gas collection It is equipped with solenoid valve on case and the tracheae of nitrogen cylinder section, drying tube and stream are equipped with successively on the tracheae of triple valve to aeration sand table end Gauge.
Further, gas gathering mask outer sheath is equipped with gas collection pipe fitting, and gas collection pipe fitting is connected to plenum chamber by delivery pipe.
Further, plenum chamber side is equipped with gas sample mouth;On-line monitoring aperture and sampling are offered on ontology cover board Mouth, water inlet pipe mouth, liquid level relay wire-socket and effluent pipe mouth, line monitor aperture and sample tap, water inlet pipe mouth, liquid level after It is equipped with perforated rubber stopper in electrical appliance wires socket and effluent pipe mouth.
Compared with prior art, the utility model has technique effect beneficial below:
A kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria of the utility model, the present apparatus pass through on reactor body Water sealing (sealing water) pipe and gas gathering mask are set on the ontology cover board at end, while realizing the recycling and its recycling to nitrogen, embodies economy Practical principle, and coordinate the multiplication characteristic and its enrichment culture mode of anaerobic ammonia oxidizing bacteria, devise a full set of automatically control Device avoids the problem of not agreeing with, and because SBR can be divided into aerobic, the methods of operation such as anaerobism, the present apparatus into reactor by exposing Nitrogen removes the oxygen in reactor to build an anaerobic environment, when the exposure oxygen into reactor or other various needs are logical When entering the gas of reactor, which is also used for other various anaerobism except anaerobic ammonia oxidizing bacteria, the enrichment culture of aerobic bacteria, together When have both the function of gas of collecting and generated in reaction, so as to further carry out qualitative, quantitative analysis to the gas of generation.
Further, reactor body upper end is equipped with lug, and lug is equipped with through-hole, and reactor body is logical with ontology cover board It crosses and is bolted, be convenient for the connective stability of reactor body and ontology cover board.
Further, upper end is equipped with buckle in babinet, is stamped cover board on the buckle of babinet, prevents moisture from quickly volatilize and dirt Object is fallen into.
Further, agitating device includes the stirring-head being placed in reactor body and the machine that is placed on the outside of reactor body Tool blender, mechanical agitator are stirring motor or stirring motor;Mechanical agitator, by gear drive, is conducive to stirring Accelerate the reaction speed of inside reactor.
Description of the drawings
FIG. 1 is a schematic structural view of the utility model.
Fig. 2 is the utility model body cap plate structure schematic diagram.
Wherein, 1, peristaltic pump, 2, immersible pump, 3, babinet, 4, heating rod, 5, low liquid level electric wire, 6, high liquid level electric wire, 7, water Tube sealing, 8, gas gathering mask, 9, reactor body, 10, plenum chamber, 11, nitrogen cylinder, 12, gas sample mouth, 13, mechanical agitator, 14, it is aerated sand table, 15, triple valve, 16, solenoid valve, 17, outlet pipe, 18, water inlet pipe, 19, agitating device, 20, timed unit, 21, attaching plug, 22, liquid level relay, 23, master switch, 24, ontology cover board, 25, gas collection tube head interface, 26, on-line monitoring hole Mouthful and sample tap, 27, water inlet pipe mouth, 28, liquid level relay wire-socket, 29, effluent pipe mouth, 30, water seal pipe sleeve, 32, with holes Rubber stopper, 33, drying tube, 34, flowmeter, 35, conveyance conduit, 36, temperature controller, 37, cover board, 38, temperature probe.
Specific implementation mode
The utility model is described in further detail below in conjunction with the accompanying drawings:
As shown in Figure 1 and Figure 2, the enrichment culture gas collecting apparatus of a kind of anaerobic ammonia oxidizing bacteria, including control device, reactor Ontology 9 and babinet 3 for 9 heating water bath of reactor body, 9 upper end of reactor body are equipped with ontology cover board 24, reactor Bottom is equipped with aeration sand table 14 in ontology 9, and ontology cover board 24 is equipped with water sealing (sealing water) pipe 7 and gas gathering mask 8, and gas gathering mask 8 passes through delivery pipe Road 35 is connected with plenum chamber 10, and aeration sand table 14 is connected with nitrogen cylinder 11 by tracheae, and aeration sand table 14 is connected to by tracheae Plenum chamber 10 is additionally provided with liquid level measurer, agitating device 19, temperature probe 38 in reactor body 9, and liquid level measurer is connected to Liquid level relay 22, temperature probe 38 are connected to temperature controller 36, and liquid level relay 22, agitating device 19 and temperature controller 36 are all connected with In control device;
9 upper end of reactor body is equipped with lug, and lug is equipped with through-hole, and reactor body 9 passes through spiral shell with ontology cover board 24 It tethers and connects;
Gas gathering mask 8 by reactor body 9 to being tapered mouth outside;Water sealing (sealing water) pipe is equipped between water sealing (sealing water) pipe 7 and ontology cover board 24 Set 30;
Upper end is equipped with buckle in babinet 3, and cover board 37 is stamped on the buckle of babinet 3, and cover board 37 is acrylic glass plate, is prevented Quickly volatilization and dirt are fallen into moisture;It is fixed with heating rod 4 on cover board 37, is used for the heating water bath of babinet 3;
Peristaltic pump 1 is connected with by water inlet pipe 18 in reactor body 9, is detested by the conveying of peristaltic pump 1 in reactor body 9 Nutrient needed for anaerobic ammonium oxidation bacterium, reactor body 9 is interior to be connected with immersible pump 2 by outlet pipe 17, and outlet pipe 17 is placed in reactor Lower end in ontology 9,17 nozzle of outlet pipe are located at sludge liquid level or more;
Control device includes control cabinet, and control cabinet is equipped with time switch 20, attaching plug 21, liquid level relay 22 and total Switch 23;
Agitating device 19 includes the stirring-head being placed in reactor body 9 and the mechanical agitation being placed on the outside of reactor body Device 13, mechanical agitator 13 are stirring motor or stirring motor;Mechanical agitator 13 passes through gear drive with stirring;
Liquid level measurer includes the low liquid level electric wire 5 being set in reactor body 9 and high liquid level electric wire 6, low liquid level electricity Line 5 is less than high liquid level electric wire 6;
The tracheae that aeration sand table 14 is connect with plenum chamber 10 and nitrogen cylinder 11 is equipped with triple valve 15, triple valve 15 to gas collection It is equipped with solenoid valve 16 on the tracheae of 11 sections of case 10 and nitrogen cylinder, is equipped with successively on the tracheae of triple valve 15 to 14 end of aeration sand table Drying tube 33 and flowmeter 34;
10 side of plenum chamber is equipped with gas sample mouth 12;
8 outer sheath of gas gathering mask is equipped with gas collection pipe fitting 25, and gas collection pipe fitting 25 is connected to plenum chamber 10 by delivery pipe 35,
On-line monitoring aperture and sample tap 26, water inlet pipe mouth 27, liquid level relay wire-socket are offered on ontology cover board 28 and effluent pipe mouth 29, line monitors aperture and sample tap 26, water inlet pipe mouth 27, liquid level relay wire-socket 28 and outlet pipe It is equipped with perforated rubber stopper 32 in mouth 29.
It is described further below in conjunction with the accompanying drawings to the structural principle of the utility model and using step:
In use, determining the period of culture and into water constituent, time switch 20 is adjusted with automatic cycling operating performance period, Nutrient needed for anaerobic ammonia oxidizing bacteria is transported to inside reactor by reactor body by peristaltic pump 1 by water inlet pipe 18, is passed through Liquid level relay 22 ensures water inlet water level, mainly by being controlled whether the connection of the electric wire of high liquid level 6 and low liquid level electric wire 5 Whether water inlet peristaltic pump stops, and after water inlet end, time switch can start 16 Open valve of solenoid valve, nitrogen cylinder 11 and plenum chamber To carrying out exposing nitrogen deoxygenation in reactor, 33 dry gas of drying tube prevents the 10 aeration sand tables 14 connected by reactor bottom Steam enters spinner flowmeter 34, and the gas flow rate of the adjustable aeration of flowmeter, by preset time, in reactor, DO, which is dropped to, needs After the numerical value wanted, time switch can close electromagnetic valve, and aeration terminates, and time switch starts mechanical agitator within the cycle of operation Make to realize uniformly mixed state in reactor;PH in reactor is monitored by pH meter Quick testing instrument, and with addition buffering Substance adjusts pH in reactor, reaches suitable pH ranges.Reactor after preset mixing cycle is stirred to react, Timing is closed mechanical agitator 13 by time switch, and after sedimentation cycle, time switch can start immersible pump 2, and reactor is logical The water outlet of outlet pipe 17 is crossed, a complete cycle terminates, and achievees the purpose that bacterial growth breeding and removes into pollutant in water.This Device by the way that water sealing (sealing water) pipe and gas gathering mask are arranged on the ontology cover board of reactor body upper end, while realize recycling to nitrogen and It is reused, and embodies economical and practical principle, and coordinate the multiplication characteristic and its enrichment culture mode of anaerobic ammonia oxidizing bacteria, It devises a full set of automatic control device and avoids the problem of not agreeing with, because SBR can be divided into aerobic, the methods of operation such as anaerobism, this Device removes the oxygen in reactor to build an anaerobic environment, when being exposed into reactor by exposing nitrogen into reactor When oxygen or other various gases for needing to be passed through reactor, which is also used for various detesting except other of anaerobic ammonia oxidizing bacteria Oxygen, the enrichment culture of aerobic bacteria, while the function of collecting the gas generated in reaction is had both, so as to further to the gas of generation Body carries out qualitative, quantitative analysis.

Claims (10)

1. a kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria, which is characterized in that including control device, reactor body (9) and for reactor body (9) heating water bath babinet (3), reactor body (9) upper end be equipped with ontology cover board (24), Reactor body (9) interior bottom is equipped with aeration sand table (14), and ontology cover board (24) is equipped with water sealing (sealing water) pipe (7) and gas gathering mask (8), collection Gas hood (8) is connected with plenum chamber (10) by conveyance conduit (35), and aeration sand table (14) is connected with nitrogen cylinder (11) by tracheae, Aeration sand table (14) is connected to plenum chamber (10) by tracheae, and liquid level measurer, agitating device are additionally provided in reactor body (9) (19), temperature probe (38), liquid level measurer are connected to liquid level relay (22), and temperature probe (38) is connected to temperature controller (36), liquid level relay (22), agitating device (19) and temperature controller (36) are all connected to control device.
2. a kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria according to claim 1, which is characterized in that control dress It sets including control cabinet, control cabinet is equipped with time switch (20), attaching plug (21), liquid level relay (22) and master switch (23), reactor body (9) upper end is equipped with lug, and lug is equipped with through-hole, and reactor body (9) passes through with ontology cover board (24) It is bolted.
3. a kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria according to claim 1, which is characterized in that gas gathering mask (8) by reactor body (9) to being tapered mouth outside;Water seal pipe sleeve (30) is equipped between water sealing (sealing water) pipe (7) and ontology cover board (24).
4. a kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria according to claim 1, which is characterized in that babinet (3) interior upper end is equipped with buckle, is stamped cover board (37) on the buckle of babinet (3), heating rod (4) is fixed on cover board (37).
5. a kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria according to claim 1, which is characterized in that reactor Peristaltic pump (1) is connected with by water inlet pipe (18) in ontology (9), passes through peristaltic pump (1) conveying anaerobism ammonia in reactor body (9) Nutrient needed for oxidation bacteria, reactor body (9) is interior to be connected with immersible pump (2) by outlet pipe (17), and outlet pipe (17) is placed in instead Answer device ontology (9) interior lower end.
6. a kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria according to claim 1, which is characterized in that stirring dress It sets (19) and includes the stirring-head being placed in reactor body (9) and the mechanical agitator (13) being placed on the outside of reactor body (9), Mechanical agitator (13) is stirring motor or stirring motor;Mechanical agitator (13) passes through gear drive with stirring.
7. a kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria according to claim 1, which is characterized in that liquid level is surveyed Measuring device includes the low liquid level electric wire (5) and high liquid level electric wire (6) being set in reactor body (9), and low liquid level electric wire (5) is less than High liquid level electric wire (6).
8. a kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria according to claim 1, which is characterized in that aeration is husky The tracheae that disk (14) is connect with plenum chamber (10) and nitrogen cylinder (11) is equipped with triple valve (15), triple valve (15) to plenum chamber (10) and on the tracheae of nitrogen cylinder (11) section it is equipped with solenoid valve (16), triple valve (15) is extremely aerated on the tracheae that sand table (14) is held It is equipped with drying tube (33) and flowmeter (34) successively.
9. a kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria according to claim 1, which is characterized in that gas gathering mask (8) outer sheath is equipped with gas collection pipe fitting (25), and gas collection pipe fitting (25) is connected to plenum chamber (10) by conveyance conduit (35).
10. a kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria according to claim 1, which is characterized in that gas collection Case (10) side is equipped with gas sample mouth (12);On-line monitoring aperture and sample tap (26), water inlet pipe are offered on ontology cover board Mouth (27), liquid level relay wire-socket (28) and effluent pipe mouth (29) monitor aperture and sample tap (26), water inlet pipe on-line It is equipped with perforated rubber stopper (32) in mouth (27), liquid level relay wire-socket (28) and effluent pipe mouth (29).
CN201721805051.7U 2017-12-21 2017-12-21 A kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria Expired - Fee Related CN207713497U (en)

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CN201721805051.7U CN207713497U (en) 2017-12-21 2017-12-21 A kind of enrichment culture gas collecting apparatus of anaerobic ammonia oxidizing bacteria

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113789277A (en) * 2021-09-15 2021-12-14 广州绿邦环境技术有限公司 Rhodococcus culture method and device for anaerobic ammonia nitrogen conversion

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113789277A (en) * 2021-09-15 2021-12-14 广州绿邦环境技术有限公司 Rhodococcus culture method and device for anaerobic ammonia nitrogen conversion

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Granted publication date: 20180810

Termination date: 20181221