CN207537455U - A kind of Rapid nucleic acid amplification system - Google Patents
A kind of Rapid nucleic acid amplification system Download PDFInfo
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- CN207537455U CN207537455U CN201721483492.XU CN201721483492U CN207537455U CN 207537455 U CN207537455 U CN 207537455U CN 201721483492 U CN201721483492 U CN 201721483492U CN 207537455 U CN207537455 U CN 207537455U
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Abstract
The utility model provides a kind of Rapid nucleic acid amplification system, the nucleic acid amplification reaction solution in reaction consumptive material is made to be shifted between different warm areas by rotation mode and realize nucleic acid rapid amplifying, overcome existing nucleic acid amplification system or time-consuming or reacts the technical issues of consumptive material is difficult to realize.Tthe utility model system includes the reaction consumptive material, temperature-controlling module and rotation support component of a kind of disc or sector.The PCR conversion zones by proximal edge are provided on reaction consumptive material, temperature-controlling module includes the PCR warm area control units of two or more sets circumference arrangement, and the temperature of PCR conversion zones on reaction consumptive material is controlled.Reaction consumptive material is placed in the support member of rotation support component, and PCR conversion zones control the transfer between different warm area control units to realize that Rapid nucleic acid expands by electric rotating machine.
Description
Technical field
The utility model is related to molecular biology and molecular diagnosis field, and in particular to a kind of quick realization nucleic acid amplification
System.
Background technology
Detection of nucleic acids is the basic experiment method of molecular biology.Molecule diagnosis is that the detection of applied molecular biology method is suffered from
In person's body or body fluid exogenous or own genetic material " nucleic acid " and its expression and make the technology of diagnosis, be contemporary doctor
Learn one of important front edge field of development.The basis of molecule diagnosis is detection and analysis for target nucleic acid, molecular diagnostic techniques
Mainly include real-time fluorescence quantitative PCR, fluorescence in situ hybridization (FISH), gene sequencing and genetic chip etc..Molecular diagnostic techniques
It is substantially all including three steps:Nucleic acid extraction purifies, nucleic acid amplification and detection.Nucleic acid amplification is target nucleic acid or target gene
The process amplified in vitro is had been developed at present including PCR (PCR), loop-mediated isothermal amplification
(LAMP), rolling circle amplification reaction (RCA), ligation amplification reaction (LCR) and recombinase polymeric enzymatic amplification (RPA) etc..PCR is most often
The method of nucleic acid in vitro amplification, it is necessity of real-time fluorescence quantitative PCR, gene sequencing and the diagnosis of genetic chip equimolecular
Link.
Most common gene-amplificative instrament and real-time fluorescence quantitative PCR be mostly in molecular biology research and clinical molecular diagnosis
It is that consumptive material is reacted based on porous PCR plate or PCR pipe etc., the heating and cooling of Metal Temperature block is controlled to realize that PCR becomes by semiconductor devices
Property, annealing and the Thermal Cycling of extension.But very long, the PCR mistakes comprising reverse transcription and pre-degeneration the time required to this kind of method
Journey is generally in 1.5 to 3 hours;By the improvement of new material, new process, this kind of method can will shorten to 1 hour the time
Within.The LightCycler 2.0 of the Roche companies and Rotor-Gene Q of Qiagen companies is heated using centrifugal type air
Thermal system, Cooling rate is very quick, and temperature uniformity is fine, and the control of this kind of method will be comprising reverse transcription and pre-degeneration
PCR processes were foreshortened within 1.5 hours, and wherein LightCycler uses capillary glass, and as reaction consumptive material, required time is only
It is 0.5-1 hours.But the temperature control accuracy of this kind of method and precision are not so good.The method that third class realizes PCR amplification is PCR
Reaction system shifts between fixed warm area, and round pcr is exactly in this way by PCR pipe in three temperature when invention
Water-bath between shift.Some existing systems by PCR reaction chambers metal bath or water-bath, oil bath different warm areas between turn
It moves or is flowed between different warm areas PCR reaction solutions by microflow control technique.The mistake of heating and cooling is not present in this method
Journey, the PCR processes comprising reverse transcription and pre-degeneration are it could even be possible to shorten within 15 minutes.But this method implements
There are many problems, first, reaction consumptive material difficult design, mechanical transfer come into full contact with warm area require to react consumptive material have it is certain
Intensity, while require good heat conductivity;Second is that transfer of the reaction consumptive material between multi-temperature zone needs complicated mechanical movement control,
Flux, the degree of automation and the size of instrument are difficult to take into account, so there is presently no the Rapid nucleic acids based on warm area branch mode
Amplification system obtains clinical practice.
Invention content
The purpose of this utility model is to provide a kind of Rapid nucleic acid amplification systems, make the nucleic acid amplification in reaction consumptive material anti-
Solution is answered to be shifted between different warm areas and realizes nucleic acid rapid amplifying, to overcome existing nucleic acid amplification system or take or be difficult to realize
The technical issues of.
To achieve these goals, the utility model provides a kind of Rapid nucleic acid amplification system, including reaction consumptive material and
Temperature-controlling module, described react are provided with PCR conversion zones on consumptive material, are characterized in that:
The PCR conversion zones are arranged on the region that reaction consumptive material leans on proximal edge;
The temperature-controlling module includes the PCR warm area control units of two or more sets circumference arrangement, and the control of PCR warm areas is single
Member controls the temperature of PCR conversion zones on reaction consumptive material, and the temperature of different PCR warm areas control unit controls can phase
Together, it can also be different;The nucleic acid amplification system further includes rotation support component, and the rotation support component includes support member
And electric rotating machine;The support member is horizontally disposed with, and is used to support fixed reaction consumptive material;The electric rotating machine supports for controlling
The rotation of component, the PCR that the PCR conversion zones of fixed reaction consumptive material are arranged in two or more sets circumference in the support member
By rotation transformation warm area between warm area control unit, Rapid nucleic acid amplification is realized.
Further, the support member is turntable;The reaction consumptive material is a disk shaped reaction consumptive material or multiple fans
Shaped reaction consumptive material, multiple fan-shaped reaction consumptive materials on the supporting member arrange by circumference.
In some embodiments, disk shaped reaction consumptive material includes the PCR units of one or more circumference uniform distributions, each
PCR units include an adding mouth that can be closed and a PCR conversion zone, and adding mouth and PCR conversion zones pass through runner
Connection, each PCR conversion zones include the PCR reaction chambers of one or more circumference uniform distributions;PCR reaction chambers are arranged at reaction consumption
On the circumference of material outmost turns.
In some embodiments, the PCR units of the disk shaped reaction consumptive material further include a PCR premix chamber or with it is more
Multiple PCR premix chambers, PCR premix chambers are connected a PCR reaction chambers with adding mouth and PCR reaction chambers by runner correspondingly.
In some embodiments, there are one the adding mouth that can be closed and one and adding mouth companies for sector reaction consumptive material tool
The PCR conversion zones connect, each PCR conversion zones include the PCR reaction chambers of one or more circle distributions;PCR reaction chambers are set
It puts on the circumference of the outmost turns of reaction consumptive material.
In some embodiments, it is provided with one between the adding mouth of the fan-shaped reaction consumptive material and multiple PCR reaction chambers
A PCR premix chambers or with multiple PCR reaction chambers multiple PCR premix chambers correspondingly, adding mouth, PCR premix chambers and PCR reactions
It is connected between chamber.
In some embodiments, the PCR warm areas control unit can be a temperature control module, and the front end of the module has
Notch forms a local air heating zone in indentation, there region by constant temperature air-flow, and it is anti-can to enter PCR therein to rotating bezel
Each PCR reaction chambers in region is answered to provide a temperature needed for PCR reactions.
In some embodiments, the PCR warm areas control unit can also include two opposite are mounted on to react consumption
The temperature control module of material upper and lower part, the temperature of two temperature control module controls are identical.The two temperature control modules are driven by driving structure
It is dynamic to clamp PCR conversion zones, provide a temperature needed for PCR reactions to each PCR reaction chambers in PCR conversion zones.
In some embodiments, the temperature control module of control annealing extension, temperature control are included in the PCR warm areas control unit
The top or lower part of module or top temperature control module or lower part temperature control module are provided with fluorescence detection unit, when PCR reaction zones
When domain rotates to the temperature control module, fluorescence detection unit carries out real-time fluorescence detection, realizes nucleic acid quantification detection function.
In some embodiments, there is clamp system or fixed card slot on the turntable, it can be by disc or sector
Reaction consumptive material be fixedly clamped on turntable and carry out rotational positioning or high speed centrifugation.
In some embodiments, the electric rotating machine is equipped with coding disk, can be to reacting the absolute position of consumptive material and turning
Speed carries out negative feedback control.The rotating speed control, rate curve can be triangular wave, sawtooth wave, sine wave or parabola
Frequency and amplitude Deng, speed curves can be with dynamic regulations, and then realize in reaction box different volumes liquid in different cavity body
Rapidly and efficiently mix.
In some embodiments, the electric rotating machine can drive reaction consumptive material to carry out high speed single direction rotation, rotating speed
Up to 2000~10000 revs/min.The liquid such as sample, template, reagent or amplified reaction solution can be driven under suitable rotating speed
Body flows to the remote cavity of distance center from the near cavity of reaction consumptive material distance center.
Compared with prior art, advantageous effect is the utility model:
1st, Rapid nucleic acid amplification system disclosed in the utility model provides the reaction consumptive material of a kind of disc or sector,
Nucleic acid amplification reaction solution, which finally all enters, to be distributed in the PCR reaction chambers of consumptive material periphery, and controlled by system in PCR warm area controls
Fast transfer realizes Rapid nucleic acid amplification between unit processed.Since there is no the process of heating and cooling, so theoretically comprising reverse
The PCR processes of record and pre-degeneration are completed it could even be possible to shortening within 15 minutes.
2nd, the reaction consumptive material of the disc of the utility model or sector design can make nucleic acid amplification reaction solution tile, more
Be conducive to the temperature transition of nucleic acid amplification reaction solution.
3rd, the Rapid nucleic acid amplification system of the utility model, by reacting the rotation of consumptive material circumferencial direction, so as to carry out not
With the switching between warm area, compared with existing reaction consumptive material warm area switching mode, there is fast speed, low noise, accurate positioning
And the features such as reproducible;In addition it can realize centrifugal fluid driving and reaction solution mixing function, the system integration is improved
Degree.
4th, the Rapid nucleic acid amplification system of the utility model, PCR warm area control units local air bath temperature control mode with
Water-bath, oil bath in background technology are compared with the temperature control mode of metal bath, fast, non-with heat exchanger effectiveness height, thermal balance speed
Contact, the features such as uniformity is good, pollution-free, lossless, the non-specificity during PCR amplification can be reduced.
5th, the utility model reaction consumptive material flexible design, can realize the Multiple detection of a sample, can also realize multiple
The Multiple detection of sample, and there is reagent pre-mixing function, the scope of application is wider.
Description of the drawings
Fig. 1 shows the floor map of one disk shaped reaction consumptive material of the utility model embodiment;
Fig. 2 represents the schematic diagram of one Rapid nucleic acid amplification system of the utility model embodiment;
Fig. 3 represents the vertical view of one Rapid nucleic acid amplification system of the utility model embodiment;
Fig. 4 represents the floor map of the fan-shaped reaction consumptive material of the utility model embodiment two;
Fig. 5 represents the schematic diagram of two Rapid nucleic acid amplification system of the utility model embodiment;
Fig. 6 represents the vertical view of two Rapid nucleic acid amplification system of the utility model embodiment;
Fig. 7 and Fig. 8 represents showing for the temperature control module switching of three Rapid nucleic acid amplification system of the utility model embodiment respectively
It is intended to;
Fig. 9 represents the floor map of second of disk shaped reaction consumptive material embodiment of the utility model;
Figure 10 represents the floor map of the third disk shaped reaction consumptive material embodiment of the utility model;
Figure 11 shows the floor map of second of fan-shaped reaction consumptive material embodiment of the utility model.
Wherein reference numeral is:21st, 31,41,51,61,71- reaction consumptive materials;22nd, 32,52,62,72- shaft card slots;23、
33rd, 53,63,73-PCR units;24th, 34,54,64,74- adding mouths;25th, 35,65,75-PCR premix chambers;26、36、56、66、
76-PCR reaction chambers;27th, 37,67,77-PCR conversion zones;28th, 38,58,68- sample-adding runners;29th, 39,69,79- liquid separation streams
Road;210th, 310- turntables;211st, 311- electric rotating machines;212nd, 312-PCR warm areas control unit;12a1- the one PCR warm areas control
Temperature control module on unit;12a2Temperature control module under-the one PCR warm area control units;12b1It is warm in-the two PCR warm area control units
Control module;12b2Temperature control module under-the two PCR warm area control units;213rd, 313- rotates fixing axle;314th, 414- optical fiber is visited
Head.
Specific embodiment
Embodiment one:
Below with reference to Fig. 1, Fig. 2, Fig. 3, a specific embodiment of the utility model is illustrated, the present embodiment
Shown in Rapid nucleic acid amplification system can load a disk shaped reaction consumptive material, and by the way of local air bath, together
The rapid amplifying of the multiple sample of nucleic acid of Shi Shixian.
As shown in Figure 1, the disk shaped reaction consumptive material 21 in the present embodiment includes a shaft card slot 22 and six PCR units
23, each PCR units 23 include an adding mouth 24, a pre-packaged PCR premix chamber 25 for having powdered reagent and three PCR
Reaction chamber 26, three PCR reaction chambers 26 constitute a PCR conversion zone 27;Adding mouth 24 is pre- by being loaded runner 28 and PCR
Mixed chamber 25 is connected;PCR premix chambers 25 are connected by liquid separation runner 29 with three PCR reaction chambers 26.
As shown in Figure 2 and Figure 3, the rotation amplification device in the present embodiment includes a turntable 210, an electric rotating machine 11
In Fig. 2 for convenience of description, with six PCR warm areas control units 212 (two of which PCR warm areas control unit is not shown).Turntable
Rotation fixing axle 213 is provided on 210, for loading, fixing reaction consumptive material 21.Electric rotating machine 211 is connected with turntable 210, can
With the reaction consumptive material 21 for driving turntable 210 and being loaded rotate or high speed centrifugation with drive reaction consumptive material 21 in liquid flow.
The front end of six PCR warm areas control units 212 is equipped with notch, and the six PCR conversion zones 27 reacted on consumptive material 21 can be embedded in
The notch.Meanwhile six PCR warm areas control units 212 provide constant temperature air-flow by the notch and form a local air bath,
In, three PCR warm areas control units 212 provide same temperature (higher temperature), and the other three PCR warm areas control unit 212 carries
For same temperature (lower temperature), high/low temperature PCR warm areas control unit 212 is alternately distributed to form the difference needed for PCR reactions
Heater strip or heating film may be used in warm area, PCR warm areas control unit 212.
The amplification process of the present embodiment is:Six identical or different liquid sample of nucleic acid are added to from adding mouth 24
It reacts in consumptive material 21, the sample added flows into PCR premix chambers 25 through being loaded runner 28, and subsequent adding mouth 24 will be sealed, instead
The system for answering consumptive material 21 that will become closing.Reaction consumptive material 21 after closing is loaded on the turntable 210 of rotation amplification device, tool
Gymnastics is fixed on by its shaft card slot 22 in the rotation fixing axle 213 of turntable as that will react consumptive material.Reaction consumptive material 21 loads
After, electric rotating machine 211 drives reaction 21 reciprocating rotary of consumptive material, and the sample of nucleic acid added in from adding mouth is made to flow into PCR premixs
The abundant mixing of PCR powdered reagents in chamber 25 and PCR premix chambers 25, subsequent electric rotating machine 211 drive reaction 21 high speed of consumptive material from
The heart rotates, and the mixed solution in PCR premix chambers 25 is made to pass through liquid separation runner 29 and enters three PCR reaction chambers 26, rotation amplification dress
It puts and six PCR warm areas control units 212 is controlled to move to six PCR conversion zones, the 27 corresponding position with reacting consumptive material 21
It puts, makes the front end notch of each PCR conversion zones 27 embedded PCR warm areas control unit 212 corresponding thereto.Finally, it rotates
Motor 211 drives the reaction reciprocating rotary between three groups of high/low temperature PCR warm areas control units 212 of consumptive material 21, provides PCR reactions institute
The different temperatures needed, to realize the rapid amplifying of sample of nucleic acid.
Embodiment two:
Below with reference to Fig. 4, Fig. 5, Fig. 6, a specific embodiment of the utility model is illustrated, this implementation column
Shown in Rapid nucleic acid amplification system can load six fan-shaped reaction consumptive materials, and by the way of being bathed using local air, simultaneously
Realize the rapid amplifying of multiple sample of nucleic acid.
As shown in figure 4, the fan-shaped reaction consumptive material 31 in the present embodiment includes two shaft card slots 32 and a PCR unit
33, PCR units 33 include an adding mouth 34, a pre-packaged PCR premix chamber 35 for having powdered reagent and three PCR reactions
Chamber 36, three PCR reaction chambers 36 constitute a PCR conversion zone 37;Adding mouth 34 is by being loaded runner 38 and PCR premix chambers
35 are connected, and PCR premix chambers 35 are connected by liquid separation runner 39 with PCR reaction chambers 36.
As shown in Figure 5, Figure 6, the rotation amplification device in the present embodiment includes a turntable 310, an electric rotating machine 311
In Fig. 5 for convenience of description, with six PCR warm areas control units 312 (two of which PCR warm areas control unit is not shown).Turntable
12 rotation fixing axles 313 are provided on 310, two rotation fixing axles 313 are one group, for loading, fixing a reaction
Consumptive material 31.Electric rotating machine 311 is connected with turntable 310, can drive turntable 310 and the reaction consumptive material 31 that is loaded rotates or at a high speed
It centrifuges that the liquid in reaction consumptive material 31 is driven to flow.The front end of six PCR warm areas control units 312 is equipped with notch, reaction consumption
PCR conversion zones 37 on material 31 can be embedded in the notch.Meanwhile six PCR warm areas control units 312 are provided by the notch
Constant temperature air-flow forms a local air bath, wherein, three PCR warm areas control units 312 provide same temperature (higher temperature),
The other three PCR warm areas control unit 312 provides same temperature (lower temperature), and high/low temperature PCR warm areas control unit 312 replaces
To form the different warm areas needed for PCR reactions, heater strip or heating film may be used in PCR warm areas control unit 312 for distribution.Together
When, installation is there are three fibre-optical probe 314 in three low temperature PCR warm areas control units 312, and for reacting PCR, to carry out multichannel glimmering
Light detection.
The amplification process of the present embodiment is:Liquid sample of nucleic acid is added to from adding mouth 34 in reaction consumptive material 31, institute
The sample of addition flows into PCR premix chambers 35 through being loaded runner 38, and subsequent adding mouth 34 will be sealed, and reaction consumptive material 31 will become sealing
The system closed.Later by six be added to identical or different sample of nucleic acid and close after reaction consumptive material 31 be loaded into rotation expand
It is installed in addition on the turntable 310 put, concrete operations are to consolidate six reaction consumptive materials 31 by its respective two shaft card slot 32 respectively
It is scheduled in six groups of rotation fixing axles 312 of turntable.After six reaction 31 loadeds of consumptive material, electric rotating machine 311 drives six instead
31 reciprocating rotary of consumptive material is answered, makes sample of nucleic acid and the abundant mixing of PCR powdered reagents in PCR premix chambers 35, subsequent electric rotating machine
311 drive six reaction consumptive material 31 high speed centrifugation rotations, and the mixed solution in PCR premix chambers 35 is made to pass through liquid separation runner 39 and is entered
Three PCR reaction chambers 36.Rotation six PCR warm areas control units 312 of amplification device control are moved to reacts consumptive material 31 with six
37 corresponding position of PCR conversion zones, make the embedded PCR warm areas control corresponding thereto of each PCR conversion zones 37 single
The front end notch of member 312.Finally, electric rotating machine 311 drive reaction consumptive material 31 three groups of high temperature PCR warm areas control units 312 it
Between reciprocating rotary, provide PCR reaction needed for different temperatures, to realize nucleic acid rapid amplifying;Whenever PCR conversion zones 37 are embedded in
During the front end notch of three low temperature PCR warm areas control units 312, three fibre-optical probes in low temperature PCR warm areas control unit 312
314 can react PCR progress multichannel fluoroscopic examination, realize nucleic acid fluorescent detection.
Embodiment three:
Below with reference to Fig. 7, Fig. 8, a specific embodiment of the utility model rotation amplification device is illustrated,
Rapid nucleic acid amplification system shown in this implementation column realizes the fast of single or multiple sample of nucleic acid by the way of temperature control module
Speed amplification.
As Figure 7-8, the rotation amplification device in the present embodiment includes a turntable (not shown), an electric rotating machine
411 and two PCR warm area control units:First PCR warm areas control unit includes upper temperature control module 12a1With lower temperature control module
12a2, the 2nd PCR warm areas control unit include upper temperature control module 12b1With lower temperature control module 12b2, about two groups temperature control module phases
To being mounted on reaction consumptive material upper and lower part, identical temperature is controlled.Two groups of temperature control modules can be driven by driving structure to be clamped
PCR conversion zones provide a temperature needed for PCR reactions to each PCR reaction chambers in PCR conversion zones;Meanwhile second
The upper temperature control module 12b of PCR warm area control units1There are five fibre-optical probes 414 for installation, glimmering for reacting PCR progress multichannel
Light detection.
The specific embodiment of the present embodiment is:After reaction consumptive material 41 is loaded into rotation amplification device, electric rotating machine 411
41 reciprocating rotary of reaction consumptive material is driven to complete the mixing of the sample in PCR premix chambers and encapsulation powdered reagent;After mixing, electric rotating
Machine 411 drives reaction consumptive material 41 quick mobile back and forth between the first PCR warm areas control unit, the 2nd PCR warm area control units,
To carry out PCR amplification, heater strip or heating film may be used in the first PCR warm areas control unit, the 2nd PCR warm area control units.
As shown in fig. 7, when the PCR conversion zones of reaction consumptive material 41 are moved to the control of the first PCR warm areas under the drive of electric rotating machine 411
During unit, the upper temperature control module 12a of the first PCR warm area control units1, lower temperature control module 12a2It is driven by driving structure and clamps PCR
Conversion zone is gone forward side by side trip temperature control;As shown in figure 8, when drive of the PCR conversion zones in electric rotating machine 411 for reacting consumptive material 41
Under when being moved to the 2nd PCR warm area control units, the upper temperature control module 12b of the 2nd PCR warm area control units1, lower temperature control module
12b2It is driven by driving structure and clamps PCR conversion zones and go forward side by side trip temperature control, provided in this way needed for PCR reactions not
It is synthermal, realize nucleic acid rapid amplifying;Whenever the upper temperature control module 12b of the 2nd PCR warm area control units1, lower temperature control module 12b2
Clamp PCR conversion zones into trip temperature control when, upper temperature control module 12b1On five fibre-optical probes 414 PCR can be reacted
Multichannel fluoroscopic examination is carried out, realizes nucleic acid fluorescent detection.
Other embodiments
Fig. 9 represents the floor map of second of disk shaped reaction consumptive material embodiment of the utility model.In the embodiment, circle
Dish type reaction consumptive material 51 includes a shaft card slot 52 and six PCR units 53, and each PCR units 53 include an adding mouth 54
With a PCR reaction chamber 56;Adding mouth 54 is connected by being loaded runner 58 with PCR reaction chambers 56.The disk shaped reaction consumptive material
The rapid amplifying that six kinds of identical or different PCR reaction solutions having had been provided with carry out nucleic acid can be added.
Figure 10 represents the floor map of the third disk shaped reaction consumptive material embodiment of the utility model.In the embodiment,
Disk shaped reaction consumptive material 61 includes a shaft card slot 62 and four PCR units 63, and each PCR units 63 include an adding mouth
64th, three pre-packaged PCR premix chambers 65 for having identical or different powdered reagent and three PCR reaction chambers 66, three PCR reaction chambers
66 constitute a PCR conversion zone 67.Adding mouth 64 is connected by being loaded runner 68 with three PCR premix chambers 65, three
PCR premix chambers 65 are connected by three runners 69 with three PCR reaction chambers 66.Four PCR of the disk shaped reaction consumptive material are mono-
Member 63 can add four identical or different sample of nucleic acid, and three PCR reaction chambers 66 of each PCR unit 63 can carry out
Three identical or different pcr amplification reactions.
Figure 11 represents the floor map of second of fan-shaped reaction consumptive material embodiment of the utility model.In the embodiment, fan
Shaped reaction consumptive material 71 includes two shaft card slots 72 and a PCR unit 73.PCR units 73 include an adding mouth 74, three
The pre-packaged PCR premix chambers 75 for having identical or different PCR powdered reagents and three PCR reaction chambers 76, three 76 structures of PCR reaction chambers
Into a PCR conversion zone 77.Adding mouth 74 is connected by being loaded runner 78 with three PCR premix chambers 75, and three PCR are pre-
Mixed chamber 75 is connected by three runners 79 with three PCR reaction chambers 76.It is anti-that four sectors can be loaded in rotation amplification device
Answer consumptive material 71, each fan-shaped reaction consumptive material 71 can add a kind of sample of nucleic acid, each sample of nucleic acid can carry out three it is identical
Or different pcr amplification reaction.
The nucleic acid sample added by adding mouth in the utility model embodiment one, embodiment two, embodiment three and Figure 10, Figure 11
Originally can be extracted, purifying sample of nucleic acid or original sample of nucleic acid, when sample of nucleic acid is original sample of nucleic acid
When, can by reaction consumptive material and premix chamber between runner structure design so that sample of nucleic acid when flowing into premix chamber
Through the sample of nucleic acid for being purifying.
Obviously, above-described embodiment of the utility model is only intended to clearly illustrate the utility model example, and
It is not the restriction to the embodiment of the utility model.It can also make on the basis of the above description other various forms of
Variation changes, and there is no necessity and possibility to exhaust all the enbodiments.And these belong to the spirit of the utility model
Among the obvious changes or variations extended out are still in the scope of protection of the utility model.
Claims (10)
1. including reaction consumptive material and temperature-controlling module, PCR is provided on the reaction consumptive material for a kind of Rapid nucleic acid amplification system
Conversion zone, it is characterised in that:
The PCR conversion zones are arranged on the region that reaction consumptive material leans on proximal edge;
The temperature-controlling module includes the PCR warm area control units of two or more sets circumference arrangement, PCR warm area control units pair
The temperature of PCR conversion zones is controlled on reaction consumptive material;
The nucleic acid amplification system further includes rotation support component, and the rotation support component includes support member and electric rotating
Machine;The support member is horizontally disposed with, and is used to support and fixes reaction consumptive material;The electric rotating machine is used to control support member
It rotates, one or more PCR conversion zones of fixed reaction consumptive material are arranged in two or more sets circumference in the support member
PCR warm area control units between pass through rotation mode convert warm area, realize nucleic acid amplification.
2. Rapid nucleic acid amplification system according to claim 1, it is characterised in that:
The support member is turntable;The reaction consumptive material reacts consumptive material for a disk shaped reaction consumptive material or multiple sectors, more
A fan-shaped reaction consumptive material on the supporting member arrange by circumference.
3. Rapid nucleic acid amplification system according to claim 2, it is characterised in that:
The disk shaped reaction consumptive material includes the PCR units of one or more circumference uniform distributions, and each PCR units include one can be with
The adding mouth of closing and a PCR conversion zone, adding mouth are connected with PCR conversion zones by runner;
Each PCR conversion zones include the PCR reaction chambers of one or more circumference uniform distributions.
4. Rapid nucleic acid amplification system according to claim 3, it is characterised in that:
Each PCR units of the disk shaped reaction consumptive material further include a PCR premix chamber or a pair of with multiple PCR reaction chambers one
The multiple PCR premix chambers answered, PCR premix chambers are connected with adding mouth and PCR reaction chambers by runner.
5. Rapid nucleic acid amplification system according to claim 2, it is characterised in that:
The fan-shaped reaction consumptive material includes an adding mouth that can be closed and a PCR conversion zone being connect with adding mouth,
The PCR conversion zones include the PCR reaction chambers of one or more circle distributions.
6. Rapid nucleic acid amplification system according to claim 5, it is characterised in that:
Between the adding mouth and multiple PCR reaction chambers of the fan-shaped reaction consumptive material setting there are one PCR premix chambers or with multiple PCR
Reaction chamber multiple PCR premix chambers correspondingly, are connected between adding mouth, PCR premix chambers and PCR reaction chambers.
7. Rapid nucleic acid amplification system according to any one of claims 1 to 6, it is characterised in that:
The PCR warm areas control unit reacts consumptive material upper and lower part for an individual temperature control module or two opposite are mounted on
Upper temperature control module and lower temperature control module,
The front end setting of individual temperature control module is jagged, and the indentation, there region is the air heating zone of temperature control module, is reacted
Circumferential area where the PCR reaction chambers of consumptive material is embedded in the indentation, there of multiple temperature control modules;
Upper temperature control module is identical with the temperature that lower temperature control module controls;Two temperature control modules drive clamping PCR anti-by driving structure
Region is answered, a temperature needed for PCR reactions is provided to each PCR reaction chambers in PCR conversion zones.
8. Rapid nucleic acid amplification system according to claim 7, it is characterised in that:
In the PCR warm areas control unit comprising control annealing extension temperature control module, the top or lower part of temperature control module or on
Portion's temperature control module or lower part temperature control module are provided with fluorescence detection unit.
9. Rapid nucleic acid amplification system according to claim 2, it is characterised in that:
There is clamp system or fixed card slot, for being fixedly clamped disc or fan-shaped reaction consumptive material in turntable on the turntable
On.
10. Rapid nucleic acid amplification system according to claim 1, it is characterised in that:
The electric rotating machine is equipped with coding disk, and the coding disk carries out negative-feedback control to the absolute position and rotating speed of reacting consumptive material
System, rate curve is triangular wave, sawtooth wave, sine wave or parabola.
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Cited By (5)
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CN109810875A (en) * | 2019-03-28 | 2019-05-28 | 苏州点晶生物科技有限公司 | A kind of multi-joint checking device of sector nucleic acid |
CN110114475A (en) * | 2018-12-20 | 2019-08-09 | 广州市华南医学研究中心 | The method and its equipment of a kind of very fast nucleic acid amplification and application |
CN110180611A (en) * | 2019-07-05 | 2019-08-30 | 科赫生物科技(北京)有限公司 | Micro-fluidic chip component |
CN112094740A (en) * | 2020-09-25 | 2020-12-18 | 壹宏(深圳)基因有限公司 | PCR amplification method and PCR amplification device |
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CN112094740A (en) * | 2020-09-25 | 2020-12-18 | 壹宏(深圳)基因有限公司 | PCR amplification method and PCR amplification device |
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