CN207435462U - A kind of hermetic multiple fluorescent PCR detection cartridge - Google Patents
A kind of hermetic multiple fluorescent PCR detection cartridge Download PDFInfo
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- CN207435462U CN207435462U CN201721331216.1U CN201721331216U CN207435462U CN 207435462 U CN207435462 U CN 207435462U CN 201721331216 U CN201721331216 U CN 201721331216U CN 207435462 U CN207435462 U CN 207435462U
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- axis
- suction nozzle
- pedestal
- pcr detection
- filter core
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Abstract
The utility model discloses a kind of hermetic multiple fluorescent PCR detection cartridges, including upper cover, housing, axis, filter core suction nozzle and pedestal, upper cover, housing and pedestal form to form an enclosure space, the centre bore that axis upper end is pierced by upper cover is connected with driving mechanism, in the centre bore of lower end inserted base, it is connected in the middle part of axis by transverse arm with filter core suction nozzle, spacing collar is provided on the axis between upper cover and transverse arm, spring is provided on the axis between pedestal and transverse arm；Several PCR pipes and several reagent troughs are provided on pedestal；The filter core suction nozzle is connected with aspirator.The hermetic multiple fluorescent PCR detection cartridge of the utility model can be by two steps of paramagnetic particle method nucleic acid extraction and fluorescence quantitative PCR detection, it is completed in a small closed box body, it is detected available for the nucleic acid extraction under no molecular diagnostic laboratories environment and fluorescent PCR, there is compact-sized, easily operated, simple in structure, easy assembling, effectively improve the environment fitness of molecule diagnostics classes product.
Description
Technical field
The utility model is related to a kind of extraction of biotinylated nucleic acid molecule and detection cartridges, are specially that a kind of hermetic multiple is glimmering
Light PCR detection cartridges.
Background technology
Nucleic acid extraction and detection are an important process of molecule diagnosis, but in the extraction of nucleic acid and detection process,
Aerosol Pollution is also easy to produce, result is made false positive occur, the diagnosis of conventional molecule is needed in specific molecular diagnostic laboratories
It carries out, seriously constrains the application and popularization of this technology.
Utility model content
The technical problems to be solved in the utility model is that existing routine molecular diagnostics needs is overcome to be examined in specific molecule
The defects of carrying out in disconnected laboratory, seriously constrain the application and popularization of this technology, provides a kind of enclosed cartridge, can use
In the research and development of POCT quasi-molecule diagnostic instrments products.
In order to solve the above-mentioned technical problem, the utility model provides following technical solution：
A kind of hermetic multiple fluorescent PCR detection cartridge, including upper cover, housing, axis, filter core suction nozzle and pedestal, on
Lid, housing and pedestal form to form an enclosure space, and the centre bore that axis upper end is pierced by upper cover is connected with driving mechanism, lower end
It in the centre bore of inserted base, is connected in the middle part of axis by transverse arm with filter core suction nozzle, axis is covered with the junction of upper cover and pedestal
Spacing collar is equipped with, spring is provided on the axis between pedestal and transverse arm；Several PCR pipes and reagent are provided on pedestal
Slot；The filter core suction nozzle is connected with aspirator.
Further, the aspirator is made of flexible tracheae and syringe.Air-path interface is provided on upper lid,
Lower air-path interface is provided on transverse arm, the flexibility tracheae is connected by upper air-path interface and lower air-path interface with filter core suction nozzle.
Preferably, the upper air-path interface both ends use pagoda header structure, convenient for coupling and sealing with flexible tracheae；
Preferably, the lower air-path interface upper end uses pagoda header structure, convenient for coupling and sealing with flexible tracheae；Lower end
Using pyramidal structure, and there is sealing ring, can combine closely with filter core suction nozzle, be not in gas leakage；
Preferably, the axis is using the structure above lower circle, it is preferred to use hard material is made.
Further, the housing is to use cylindrical housings.
Further, reagent trough, PCR pipe, use is transparent, semi-flexible, heat safe material.
When axis under the action of driving mechanism when rotating, upper cover and filter core suction nozzle synchronously rotate, meanwhile,
Under axis and spring effect, filter core suction nozzle can also move up and down with axis, so as to complete to different reagent troughs and PCR pipe
Interior liquid is shifted.Since upper cover and filter core suction nozzle synchronously rotate, the space of cartridge can be saved.
Different detection reagents can be added in reagent trough and PCR pipe, rotation central axis draws reagent trough using filter core suction nozzle
Liquid carries out reagent tank liquid pressure-vaccum mixing, and cooperation magnetic material can realize the combination, repeatedly clear of magnetic bead and nucleic acid
It washes, elute, complete nucleic acid extraction and purifying.It can be added to afterwards by extracting with nucleic acid after purification in PCR pipe, addition reagent is simultaneously
Coordinate the hot well of matched fluorescent quantitative PCR detector, available for fluorescence quantitative PCR detection.
The hermetic multiple fluorescent PCR detection cartridge of the utility model can be by paramagnetic particle method nucleic acid extraction and fluorescent quantitation
PCR detects two steps, is completed in a small closed box body, available for the core under no molecular diagnostic laboratories environment
Acid extraction and fluorescent PCR detection, have many advantages, such as compact-sized, easily operated, simple in structure, easy assembling, effectively improve molecule
The environment fitness of diagnostics classes product.
Description of the drawings
Attached drawing is used to provide a further understanding of the present invention, and a part for constitution instruction, with this practicality
New embodiment for explaining the utility model, does not form the limitation to the utility model together.In the accompanying drawings：
Fig. 1 is the structure diagram of the utility model；
Fig. 2 is the part-structure schematic diagram of the utility model.
Specific embodiment
The preferred embodiment of the utility model is illustrated below in conjunction with attached drawing, it should be understood that described herein excellent
Embodiment is selected to be only used for describing and explaining the present invention, is not used to limit the utility model.
Embodiment
As shown in Figure 1, a kind of hermetic multiple fluorescent PCR detection cartridge, including upper cover 1, cylindrical housings 9, axis
13rd, filter core suction nozzle 6 and pedestal 12, upper cover 1, cylindrical housings 9 and pedestal 12, which are formed, forms an enclosure space, 13 upper end of axis
The centre bore for being pierced by upper cover 1 is connected with driving mechanism, and in the centre bore of lower end inserted base 12,13 middle part of axis passes through transverse arm 5
Be connected with filter core suction nozzle 6, the junction set of axis 13 and upper cover 1 and pedestal 12 is equipped with spacing collar 7, pedestal 12 and transverse arm 5 it
Between axis 13 on be provided with spring 8；6 PCR pipes 10 and reagent trough 11 are provided on pedestal 12；Filter core suction nozzle 6 and flexible air
Pipe 3 connects.Air-path interface 2 is provided in upper cover 1, lower air-path interface 4 is provided on transverse arm 5；Flexible tracheae one end 3 is by upper
Air-path interface 2 and lower air-path interface 4 are connected with filter core suction nozzle 6, and the other end is connected with syringe.
Upper 2 both ends of air-path interface use pagoda header structure, convenient for coupling and sealing with flexible tracheae；On lower air-path interface 4
End is using pagoda header structure, convenient for coupling and sealing with flexible tracheae；Lower end uses pyramidal structure, and has sealing ring, Ke Yiyu
Filter core suction nozzle is combined closely, and is not in gas leakage；Axis is using the structure of lower circle top made of hard material, 11 He of reagent trough
PCR pipe 10 is made of soft heat-resisting material.Upper cover, pedestal, axis, transverse arm, upper air pipe interface, lower air pipe interface and circle
Shape shell is formed using hard transparent plastic injection molding.
The mixed liquor (1000 μ L) of cracking, combination and magnetic bead is placed in reagent trough 1, and adds in 300 μ L of sample to be tested, is tried
Place cleaning solution 1 (300 μ L) in agent slot 2, placement cleaning solution 2 (300 μ L) in 3 hole of reagent trough, not liquid feeding in reagent trough 4, reagent
Eluent (100 μ L) is placed in slot 5, mineral oil (1000 μ L) is placed in 6 hole of reagent trough, being placed respectively in PCR pipe can detect
The PCR reaction solution (40 μ L) of different purpose nucleic acid segments.It is inserted in spacing collar, transverse arm, spacing collar successively on axis, is led with flexibility
Pipe couples the lower port of upper air pipe interface with the upper port of lower air pipe interface, and filter core suction nozzle is inserted into the lower end of lower air pipe interface
Mouthful, and insert firmly, and do encapsulation process, it is ensured that it is air tight.Cylindrical container is placed on pedestal, the axis worked good first is inserted into
Upper cover forms assembly, then the whole loading above circular housing, the flexible tracheae of upper port and syringe of upper gas-tpe fitting
Connection, completes the assembling of entire cartridge.
Body operating process is as follows：
Filter core suction nozzle is directed at reagent trough 1, is then pressed downward axis, the liquid that suction nozzle is inserted in the hole by the 1st, rotation central axis
Interior, pull syringe after the liquid in device to hole carries out pressure-vaccum mixing, stands 10 minutes, the cell cracking in sample to be tested, nucleic acid
It is released in liquid, magnetic bead is combined under the action of liquid is combined with nucleic acid；
2nd, magnet is placed in 1 bottom of reagent trough, magnetic bead is adsorbed, after absorption, filter core suction nozzle is by liquid in reagent trough 1
It suctions out, is transferred in 4 holes, the magnetic bead combined with nucleic acid is retained in reagent trough；
3rd, the cleaning solution 1 in 2 hole of reagent trough is all added in 1 hole of reagent trough with filter core suction nozzle, magnet is removed, with filter
Liquid, which inhale, in wicking head device to hole blows mixing, and magnetic bead surfaces are combined except epinucleic other impurities are cleaned；
4th, after cleaning, magnet is placed on to the bottom of reagent trough 1, magnetic bead is adsorbed, after absorption, filter core suction nozzle will try
Liquid suctions out in agent slot 1, is transferred in reagent trough 2；
5th, the cleaning solution 2 in 3 hole of reagent trough is all added in reagent trough 1 with filter core suction nozzle, removes magnet, use filter core
Suction nozzle, which inhale, blows mixing, and magnetic bead surfaces are combined except epinucleic other impurities carry out secondary cleaning；
6th, after cleaning, magnet is placed on to the bottom of reagent trough 1, magnetic bead is adsorbed, after absorption, filter core suction nozzle will try
Liquid suctions out in agent slot 1, is transferred in reagent trough 3；
7th, the elution in reagent trough 5 is all added in reagent trough 1 with filter core suction nozzle, removes magnet, with filter core suction nozzle
Liquid, which inhale, in device to hole blows mixing, the Nucleic Acid Elution that magnetic bead surfaces are combined；
8th, after eluting, magnet is placed on to the bottom of reagent trough 1, magnetic bead is adsorbed, after absorption, filter core suction nozzle will try
Liquid suctions out in agent slot 1, is transferred in reagent trough 5, and the liquid in reagent trough 5 contains extraction and nucleic acid after purification, can use
In fluorescence quantitative PCR detection；
9th, using filter core suction nozzle, take the nucleic acid solution of the purifying of 10 μ L respectively from reagent trough 5, be added to each PCR pipe
Interior (noticing that suction nozzle cannot be inserted into the liquid in PCR pipe)；
10 then utilize filter core suction nozzle, take the mineral oil of 50 μ L respectively from reagent trough 6, be added in each PCR pipe
(noticing that suction nozzle cannot be inserted into the liquid in PCR pipe), completes the preparation of PCR reaction systems；
11st, cartridge is positioned in the hot well of matched fluorescent quantitative PCR detector, proceeds by fluorescent quantitation
The detection of PCR.
This set mechanism can allow suction nozzle in close box body, and the liquid in different reagent troughs and PCR pipe can be shifted.
Cartridge can be used for no molecular diagnostic laboratories environment under nucleic acid extraction and fluorescent PCR detection, have it is compact-sized, be easy to grasp
Make, environment tolerance is high, can detect the advantages that multiple indexs.
Finally it should be noted that：The above is only the preferred embodiment of the utility model only, is not limited to this
Utility model, although the utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art
For, it can still modify to the technical solution recorded in foregoing embodiments or to which part technical characteristic
Carry out equivalent substitution.Within the spirit and principle of the utility model, any modifications, equivalent replacements and improvements are made,
It should be included within the scope of protection of this utility model.
Claims (7)
1. a kind of hermetic multiple fluorescent PCR detection cartridge, which is characterized in that including upper cover, housing, axis, filter core suction nozzle
And pedestal, upper cover, housing and pedestal form to form an enclosure space, axis upper end is pierced by the centre bore and driving mechanism of upper cover
Connection in the centre bore of lower end inserted base, is connected in the middle part of axis by transverse arm with filter core suction nozzle, axis and upper cover and pedestal
Junction set is equipped with spacing collar, and spring is provided on the axis between pedestal and transverse arm；Several PCR pipes are provided on pedestal
With several reagent troughs；The filter core suction nozzle is connected with aspirator.
2. hermetic multiple fluorescent PCR detection cartridge as described in claim 1, which is characterized in that the aspirator
It is made of flexible tracheae and syringe.
3. hermetic multiple fluorescent PCR detection cartridge as claimed in claim 2, which is characterized in that be provided on upper lid
Air-path interface, lower air-path interface is provided on transverse arm, and the flexibility tracheae passes through upper air-path interface and lower air-path interface and filter core
Suction nozzle connects.
4. hermetic multiple fluorescent PCR detection cartridge as claimed in claim 3, which is characterized in that the upper air-path interface
Both ends use pagoda header structure.
5. the hermetic multiple fluorescent PCR detection cartridge as described in claim 3 or 4, which is characterized in that the lower gas circuit connects
Mouth upper end uses pyramidal structure using pagoda header structure, lower end.
6. hermetic multiple fluorescent PCR detection cartridge as described in claim 1, which is characterized in that under the axis uses
The structure of circle top.
7. hermetic multiple fluorescent PCR detection cartridge as described in claim 1, which is characterized in that the housing is with circle
Cylindrical shell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721331216.1U CN207435462U (en) | 2017-10-16 | 2017-10-16 | A kind of hermetic multiple fluorescent PCR detection cartridge |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721331216.1U CN207435462U (en) | 2017-10-16 | 2017-10-16 | A kind of hermetic multiple fluorescent PCR detection cartridge |
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| Publication Number | Publication Date |
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| CN207435462U true CN207435462U (en) | 2018-06-01 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107653301A (en) * | 2017-10-16 | 2018-02-02 | 南京中科拜尔医学技术有限公司 | A kind of hermetic multiple fluorescent PCR detection cartridge |
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2017
- 2017-10-16 CN CN201721331216.1U patent/CN207435462U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107653301A (en) * | 2017-10-16 | 2018-02-02 | 南京中科拜尔医学技术有限公司 | A kind of hermetic multiple fluorescent PCR detection cartridge |
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