CN207435437U - A kind of circulating tumor cell piece-rate system - Google Patents
A kind of circulating tumor cell piece-rate system Download PDFInfo
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- CN207435437U CN207435437U CN201721445768.5U CN201721445768U CN207435437U CN 207435437 U CN207435437 U CN 207435437U CN 201721445768 U CN201721445768 U CN 201721445768U CN 207435437 U CN207435437 U CN 207435437U
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- tumor cell
- pedestal
- circulating tumor
- centrifugal device
- rate system
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Abstract
The utility model discloses a kind of circulating tumor cell piece-rate system, including filter device, centrifugal device and pedestal, funnel-form is in the filter device lower end and can be connected with centrifugal device upper end, filter device includes 8 μm of polycarbonate leaching films of intermediate installation, upper end is the filtering cover being connected through a screw thread, filtering cover upper end is provided with sample added holes and port lid, the centrifugal device upper side is provided with the medicament added holes with check valve, scale is provided on centrifugal device, storage groove and centrifugation turntable are set respectively on the pedestal, it is provided on the centrifugation turntable and the matched mounting groove of centrifugal device, the centrifugation turntable one side of the pedestal is provided with control display screen, switch, the side of pedestal is provided with power interface.The utility model circulating tumor cell piece-rate system by filter device and centrifugal device, can efficiently, efficiently be isolated the circulating tumor cell in peripheral blood, and its is of simple structure and low cost.
Description
Technical field
The utility model is related to technical field of medical examination, and in particular to a kind of circulating tumor cell piece-rate system.
Background technology
Circulating tumor cell (circulating tumor cells, CTCs) refers to spontaneous or because operation of diagnosis and treatment is by entity
Knurl or transfer stove discharge into the tumour cell of Peripheral Circulation, are that malignant tumor patient postoperative recurrence and DISTANT METASTASES IN occurs
Major reason.In recent years, continuously improving with detection technique, CTCs are detected as a kind of new non-invasive diagnostic work
Tool has become clinical in the application value of early detection patient postoperative recurrence and DISTANT METASTASES IN, assessment curative effect and prognosis etc.
The hot spot of research.
Since quantity of the CTCs in peripheral blood is few, need to usually be sought in about 100,000,000 leucocytes and 50,000,000,000 red blood cells
It looks for only several tumour cells, therefore for the recall rate for improving CTCs, row CTCs need to usually be enriched with before detection, it will
CTCs is separated from blood or other samples.The enrichment method of CTCs is broadly divided into biochemistry separation by its principle at present
The concentration method of method, physical property based on CTCs.
Density-gradient centrifugation method based on physical property can separate mononuclearcell (including CTCs), equipment from peripheral blood
Of less demanding, method is relatively simple, but this method is used alone and lacks specificity, easily leads to the tumour cell for lacking corresponding density
It loses.Filtration method is partition method (the isolation by size of epithelial based on tumour cell size
Tumorcells, ISET), tumour cell is mainly separated according to the of different sizes of tumour cell and normal cell.It is this to pass through
The cellular morphology of physical method for separation preserves completely, and the various antigens or molecular labeling on surface do not influence cell without destruction
Characteristic provides good condition for subsequent detection, and this method equipment and technology is of less demanding, and process is easy to grasp, still
There is also the problems such as purity is low, effect is undesirable.Then there is separation effect in the magnetic activated cell seperation based on surface specific molecule
The problems such as rate.In recent years a series of circulating tumor cell core has been developed by the way that microflow control technique is combined with antibody capture
Piece capture technique can keep the activity of CTC, but cost is relatively high.
Efficient, to efficiently separate circulating tumor cell system can be realized with relatively low cost therefore, it is necessary to a kind of.
Utility model content
The purpose of the utility model is to overcome the above-mentioned problems in the prior art, provide a kind of circulating tumor cell
Piece-rate system by filter device and centrifugal device, can efficiently, efficiently separate the circulating tumor cell in peripheral blood
Go out, and its is of simple structure and low cost.
To realize above-mentioned technical purpose and the technique effect, the utility model is to be achieved through the following technical solutions：
A kind of circulating tumor cell piece-rate system, including filter device, centrifugal device and pedestal, the filter device lower end
It can be connected for funnel-form and with centrifugal device upper end, filter device includes 8 μm of polycarbonate leaching films, upper ends of intermediate installation
For the filtering cover being connected through a screw thread, filtering cover upper end is provided with sample added holes and port lid, the centrifugal device upper end
Side is provided with the medicament added holes with check valve, and scale is provided on centrifugal device, sets storage recessed respectively on the pedestal
Slot and centrifugation turntable, described centrifuge are provided with and the matched mounting groove of centrifugal device, the centrifugation turntable one of the pedestal on turntable
Side is provided with control display screen, switch, and the side of pedestal is provided with power interface.
Preferably, the filter device lower end is provided with boss and funnel-form pipeline and can closely connect with centrifugal device upper end
It connects.
Preferably, the filter device is hollow structure, is internally provided with 8 μm of polycarbonate leaching films and places boss.
Preferably, the centrifugal device is hollow cylindrical tube, and lower end is arc-shaped.
Preferably, centrifuged on the pedestal in turntable and be provided with micromachine, micromachine can by control display screen, open
Close control.
Preferably, stored on the pedestal and detected sample, test agents and remaining detection articles for use are placed in groove.
The beneficial effects of the utility model are：The utility model circulating tumor cell piece-rate system, be provided with filter device,
Centrifugal device and pedestal can meet the separated demand of circulating tumor cell, and wherein filter device and centrifugal device passes through Xun Huan
The filtering of the density gradient centrifugation of tumour cell, 8 μm of polycarbonate leaching films, can be high by the circulating tumor cell in peripheral blood
Effect is efficiently isolated, and its is of simple structure and low cost.
Certainly, any product for implementing the utility model does not necessarily require achieving all the advantages described above at the same time.
Description of the drawings
In order to illustrate more clearly of the technical solution of the utility model embodiment, make required for being described below to embodiment
Attached drawing is briefly described, it should be apparent that, the accompanying drawings in the following description is only some embodiments of the utility model,
For those of ordinary skill in the art, without creative efforts, can also be obtained according to these attached drawings
Other attached drawings.
Fig. 1 is the structure diagram of circulating tumor cell piece-rate system described in the utility model；
In attached drawing, parts list represented by the reference numerals is as follows：
1- filter devices, 101-8 μm of polycarbonate leaching film, 102- filtering covers, 103- samples added holes and port lid, 2- from
Center device, 201- medicament added holes, 202- scales, 3- pedestals, 301- storage grooves, 302- centrifugation turntables, 303- mounting grooves,
304- power interfaces, 305- control display screens, 306- switches.
Specific embodiment
The following is a combination of the drawings in the embodiments of the present utility model, and the technical scheme in the embodiment of the utility model is carried out
It clearly and completely describes, it is clear that the described embodiments are only a part of the embodiments of the utility model rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are without creative efforts
The all other embodiment obtained, shall fall within the protection scope of the present invention.
Refering to Figure 1, the utility model is a kind of circulating tumor cell piece-rate system, including filter device 1, centrifugation
Device 2 and pedestal 3, funnel-form is in 1 lower end of filter device and can be connected with 2 upper end of centrifugal device, and filter device 1 wraps
8 μm of polycarbonate leaching films 101, upper ends for including intermediate installation are the filtering cover 102 being connected through a screw thread, and are filtered in cover 102
End is provided with sample added holes and port lid 103, and 2 upper side of centrifugal device is provided with the medicament added holes with check valve
201, scale 202 is provided on centrifugal device 2, storage groove 301 and centrifugation turntable 302 are set respectively on the pedestal 3, it is described
It is provided on centrifugation turntable 302 and is set with 2 matched mounting groove 303 of centrifugal device, 302 one side of centrifugation turntable of the pedestal 3
There are control display screen 305, switch 306, the side of pedestal 3 is provided with power interface 304.
Wherein, 1 lower end of filter device is provided with boss and funnel-form pipeline and can be closely connected with 2 upper end of centrifugal device.
Wherein, filter device 1 is hollow structure, is internally provided with 8 μm of polycarbonate leaching films 102 and places boss.
Wherein, centrifugal device 2 is hollow cylindrical tube, and lower end is arc-shaped.
Wherein, centrifuged on pedestal 3 in turntable 302 and be provided with micromachine, micromachine can by control display screen 305,
306 control of switch.
Wherein, stored on pedestal 3 and detected sample, test agents and remaining detection articles for use are placed in groove 301.
Embodiment
The utility model circulating tumor cell piece-rate system is provided with filter device 1, centrifugal device 2 and pedestal 3, uses
When, filter device 1 and centrifugal device 2 connect；The detected sample for storing storage in groove 301 is taken out, by filtering cover
102 upper ends are provided with sample added holes and port lid 103 and sample to be tested are added to filter device 1, and filter device 1 is poly- by 8 μm
Carbonic ester filter membrane 102 separates part circulating tumor cell on 8 μm of polycarbonate leaching films 102, haemocyte and remaining Xun Huan
Tumour cell then flows to the bottom of centrifugal device, and the medicament added holes 201 with check valve is provided with from 2 upper side of centrifugal device
Density gradient centrifugation medicament is added, then centrifugal device 2 is mounted on the mounting groove 303 of centrifugation turntable 302 of pedestal 3, is led to
Cross control display screen 305, circulating tumor cell remaining after filtering is separated in 306 control centrifugation of switch；The utility model
The separated demand of circulating tumor cell, the density that wherein filter device 1 and centrifugal device 2 pass through circulating tumor cell can be met
The filtering of gradient centrifugation, 8 μm of polycarbonate leaching films 101 can efficiently, efficiently separate the circulating tumor cell in peripheral blood
Go out, and its is of simple structure and low cost.
In the description of this specification, the description of reference term " one embodiment ", " example ", " specific example " etc. means
At least one of the utility model is contained in reference to the embodiment or example particular features, structures, materials, or characteristics described
In embodiment or example.In the present specification, schematic expression of the above terms be not necessarily referring to identical embodiment or
Example.Moreover, particular features, structures, materials, or characteristics described can be in any one or more embodiments or example
In combine in an appropriate manner.
The preferred embodiment in the utility model disclosed above is only intended to help to illustrate the utility model.Preferred embodiment is simultaneously
There is no the details that detailed descriptionthe is all, it is only the specific embodiment also not limit the utility model.Obviously, according to this theory
The content of bright book can make many modifications and variations.This specification is chosen and specifically describes these embodiments, is in order to preferably
The principle and practical application of the utility model is explained, so that skilled artisan can be best understood by and utilize this
Utility model.The utility model is limited only by the claims and their full scope and equivalents.
Claims (6)
1. a kind of circulating tumor cell piece-rate system, it is characterised in that：Including filter device, centrifugal device and pedestal, the mistake
Funnel-form is in filter device lower end and can be connected with centrifugal device upper end, and filter device includes 8 μm of poly- carbonic acid of intermediate installation
Ester filter membrane, upper end are the filtering cover being connected through a screw thread, and filtering cover upper end is provided with sample added holes and port lid, it is described from
Center device upper side is provided with the medicament added holes with check valve, and scale is provided on centrifugal device, is distinguished on the pedestal
Set storage groove and centrifugation turntable, it is described centrifugation turntable on be provided with the matched mounting groove of centrifugal device, the pedestal
Centrifugation turntable one side is provided with control display screen, switch, and the side of pedestal is provided with power interface.
2. a kind of circulating tumor cell piece-rate system as described in claim 1, it is characterised in that：The filter device lower end is set
Being equipped with boss and funnel-form pipeline can closely be connected with centrifugal device upper end.
3. a kind of circulating tumor cell piece-rate system as described in claim 1, it is characterised in that：The filter device is hollow
Structure is internally provided with 8 μm of polycarbonate leaching films and places boss.
4. a kind of circulating tumor cell piece-rate system as described in claim 1, it is characterised in that：The centrifugal device is hollow
Cylindrical tube, lower end are arc-shaped.
5. a kind of circulating tumor cell piece-rate system as described in claim 1, it is characterised in that：Turntable is centrifuged on the pedestal
Micromachine is inside provided with, micromachine can be controlled by control display screen, switch.
6. a kind of circulating tumor cell piece-rate system as described in claim 1, it is characterised in that：Groove is stored on the pedestal
Inside it is placed with detected sample, test agents and remaining detection articles for use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721445768.5U CN207435437U (en) | 2017-11-02 | 2017-11-02 | A kind of circulating tumor cell piece-rate system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721445768.5U CN207435437U (en) | 2017-11-02 | 2017-11-02 | A kind of circulating tumor cell piece-rate system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207435437U true CN207435437U (en) | 2018-06-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721445768.5U Expired - Fee Related CN207435437U (en) | 2017-11-02 | 2017-11-02 | A kind of circulating tumor cell piece-rate system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207435437U (en) |
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2017
- 2017-11-02 CN CN201721445768.5U patent/CN207435437U/en not_active Expired - Fee Related
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180601 Termination date: 20181102 |
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| CF01 | Termination of patent right due to non-payment of annual fee |