CN207418758U - A kind of stem cell culture perfusing device - Google Patents
A kind of stem cell culture perfusing device Download PDFInfo
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- CN207418758U CN207418758U CN201721406242.6U CN201721406242U CN207418758U CN 207418758 U CN207418758 U CN 207418758U CN 201721406242 U CN201721406242 U CN 201721406242U CN 207418758 U CN207418758 U CN 207418758U
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- end cap
- perfusion
- connecting rod
- stem cell
- cell culture
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Abstract
The utility model provides a kind of stem cell culture perfusing device,Including perfusion bottle,End cap,Connecting rod,Return spring,Piston,Water tank,Hose,Electric telescopic rod,Annular conduit and fan,End cap is placed in perfusion bottle upper end,And end cap is mounted on connecting rod annular side,Return spring upper end is fixed on end cap lower end,And return spring lower end is mounted on connecting rod annular side medium position,Piston is fixed on connecting rod lower end,The design can stop perfusion at any time,Water tank is fixed on end cap upper surface,Hose is assemblied in water tank right end,Electric telescopic rod upper end is fixed on end cap lower face,Electric telescopic rod lower end is equipped with annular conduit,Hose lower end is connected through end cap with annular conduit,Fan is assemblied in end cap lower face,The design achieves the functions of cleaning perfusion bottle,The utility model is highly practical,It is easy to operate,Security is good,Cleannes are high,It is easy to use,Reliability is high.
Description
Technical field
The utility model is a kind of stem cell culture perfusing device, belongs to biological technical field.
Background technology
Stem cell is a kind of multipotential cell with the of self-replication capacity, and under certain condition, it can be divided into more
Kind functioning cell, the stage of development according to residing for stem cell is divided into embryonic stem cell and adult stem cell, according to the hair of stem cell
Potential is educated to be divided into three classes；Myeloid-lymphoid stem cell, multipotential stem cell and unipotent stem cell, stem cell are a kind of inabundant differentiation, still not
Ripe cell, has the potential function for regenerating various histoorgans and human body, and medical field is known as " general-purpose cell ".
In the prior art, stem cell culture perfusing device is similar to funnel, is unable to control stem cell perfusion quantity, influences to train
Efficiency is supported, while lacks cleaning function in perfusion device, easily pollutes stem cell, dry cell mass is reduced, so needing one kind
Stem cell culture perfusing device solves problem above.
Utility model content
In view of the deficienciess of the prior art, the utility model aim is to provide a kind of stem cell culture perfusing device, with
The problems mentioned above in the background art are solved, the utility model is highly practical, easy to operate, and security is good, and cleannes are high, make
With convenient, reliability height.
To achieve these goals, the utility model is to realize by the following technical solutions：A kind of stem cell culture
Perfusion device, including perfusion bottle, perfusion mechanism and cleaning mechanism, the perfusion bottle upper end installation perfusion mechanism, the cleaning
Mechanism is assemblied in perfusion mechanism upper surface, and the perfusion mechanism includes end cap, connecting rod, return spring and piston, the end
Lid is placed in perfusion bottle upper end, and end cap is mounted on connecting rod annular side, and the return spring upper end is fixed on end cap lower end,
And return spring lower end is mounted on connecting rod annular side medium position, the piston is fixed on connecting rod lower end, the perfusion
Bottle is internally provided with connecting rod, return spring and piston, and the cleaning mechanism includes water tank, hose, electric telescopic rod, annular water
Pipe and fan, the water tank are fixed on end cap upper surface, and the hose is assemblied in water tank right end, the electric telescopic rod upper end
End cap lower face is fixed on, the electric telescopic rod lower end is equipped with annular conduit, and the hose lower end passes through end cap and annular
Water pipe is connected, and the fan is assemblied in end cap lower face, and annular conduit, the annular conduit outside are equipped on the outside of the fan
Equipped with electric telescopic rod.
Further, the perfusion bottle lower end is equipped with outlet tube, and the perfusion bottle is connected by outlet tube with culture dish
It connects, the piston is arranged on outlet tube inner surface.
Further, the end cap upper surface offers perfusion hole, and the end cap upper surface offers through hole, and in through hole
Sliding sleeve is installed, the connecting rod is connected by sliding sleeve and through hole with end cap.
Further, the connecting rod upper end is equipped with handle, and handle is connected by fixing bolt with connecting rod, and
Handle outer end is equipped with anti-skid chequer.
Further, the water tank inner bottom is equipped with water pump, and the hose left end is connected through water tank with water pump,
The annular conduit lower face is uniformly equipped with atomizer more than two, and annular conduit inner surface is equipped with annular-heating
Piece.
Further, the electric telescopic rod and fan are equipped with two groups, and electric telescopic rod described in two groups is symmetrically fixed
In annular conduit upper end, fan described in two groups is symmetrically mounted on end cap lower face.
The beneficial effects of the utility model：A kind of stem cell culture perfusing device of the utility model, because of the utility model
End cap, connecting rod, return spring and piston are with the addition of, which can stop perfusion at any time, preferably control perfusion flow,
It prevents that perfusion flow is exceeded, improves the efficiency of stem cell culture, avoid in traditional stem cell culture perfusing device, it can not temporarily at any time
Stop perfusing course, easily because staff neglects, stem cell extra in perfusion device is caused all to be added in culture dish, is led
It causes stem cell population excessive, since the nutrition liquid proportional in culture dish is fixed, stem cell culture efficiency is caused to decline, influence dry thin
The situation of intracellular growth.
Because the utility model is with the addition of water tank, hose, electric telescopic rod, annular conduit and fan, the design achieves
The function of perfusion bottle is cleaned, improves the cleannes of perfusion bottle, and then improves perfusion quality, solves conventional dry cell culture filling
Lack cleaning function in stream device, be easy to cause dust impurity and entered by perfusion bottle in culture dish, pollute stem cell, reduce dry
The problem of cell culture efficiency.
Because being with the addition of outlet tube, the design achieves the outputs of stem cell, and because being with the addition of perfusion hole, the design is convenient for dry thin
The addition of born of the same parents, because being with the addition of sliding sleeve, the design achieves the movements of connecting rod, because being with the addition of handwheel and anti-skid chequer, the design
Convenient for controlling connecting rod, because being with the addition of water pump, the design is convenient for the water in extraction water tank, because being with the addition of atomizer, the design
The function of uniform moisture sprinkling is realized, because being with the addition of annular-heating piece, which can accelerate moisture evaporation, improve cleaning effect
Rate, the utility model is highly practical, easy to operate, and security is good, and cleannes are high, easy to use, and reliability is high.
Description of the drawings
Upon reading the detailed description of non-limiting embodiments with reference to the following drawings, other spies of the utility model
Sign, objects and advantages will become more apparent upon：
Fig. 1 is a kind of structure diagram of stem cell culture perfusing device of the utility model；
Fig. 2 is the schematic diagram of perfusion mechanism in a kind of stem cell culture perfusing device of the utility model；
Fig. 3 is the schematic diagram of cleaning mechanism in a kind of stem cell culture perfusing device of the utility model；
In figure：1- perfusions bottle, 2- perfusions mechanism, 3- cleaning mechanisms, 21- end caps, 22- connecting rods, 23- return springs,
24- pistons, 31- water tanks, 32- hoses, 33- electric telescopic rods, 34- annular conduits, 35- fans.
Specific embodiment
Technical means, creative features, achievable purpose and effectiveness to realize the utility model are easy to understand, below
With reference to specific embodiment, the utility model is expanded on further.
- Fig. 3 is please referred to Fig.1, the utility model provides a kind of technical solution：A kind of stem cell culture perfusing device, including
Perfusion bottle 1, perfusion mechanism 2 and cleaning mechanism 3,1 upper end of perfusion bottle installation perfusion mechanism 2, cleaning mechanism 3 is assemblied in perfusion machine
2 upper surface of structure.
Perfusion mechanism 2 includes end cap 21, connecting rod 22, return spring 23 and piston 24, and end cap 21 is placed in perfusion bottle
1 upper end, and end cap 21 is mounted on 22 annular side of connecting rod, 23 upper end of return spring is fixed on 21 lower end of end cap, and return bullet
23 lower end of spring is mounted on 22 annular side medium position of connecting rod, and piston 24 is fixed on 22 lower end of connecting rod, is set inside perfusion bottle 1
There are connecting rod 22, return spring 23 and piston 24, which can stop perfusion, preferably control perfusion flow at any time, prevent
Only perfusion flow is exceeded, improves the efficiency of stem cell culture.
Cleaning mechanism 3 includes water tank 31, hose 32, electric telescopic rod 33, annular conduit 34 and fan 35, and water tank 31 is solid
21 upper surface of end cap is scheduled on, hose 32 is assemblied in 31 right end of water tank, and 33 upper end of electric telescopic rod is fixed on 21 lower face of end cap, electricity
Dynamic 33 lower end of telescopic rod is equipped with annular conduit 34, and 32 lower end of hose is connected through end cap 21 with annular conduit 34, fan 35
21 lower face of end cap is assemblied in, 35 outside of fan is equipped with annular conduit 34, and 34 outside of annular conduit is equipped with electric telescopic rod 33, should
Design realizes the function of cleaning perfusion bottle 1, improves the cleannes of perfusion bottle 1, and then improves perfusion quality.
1 lower end of perfusion bottle is equipped with outlet tube, and perfusion bottle 1 is connected by outlet tube with culture dish, and piston 24 is arranged on
Outlet tube inner surface, 21 upper surface of end cap offer perfusion hole, and 21 upper surface of end cap offers through hole, and cunning is equipped in through hole
Set, connecting rod 22 are connected by sliding sleeve and through hole with end cap 21, and 22 upper end of connecting rod is equipped with handle, and handle passes through admittedly
Determine bolt with connecting rod 22 to be connected, and handle outer end is equipped with anti-skid chequer, 31 inner bottom of water tank is equipped with water pump, hose 32
Left end is connected through water tank 31 with water pump, and 34 lower face of annular conduit is uniformly equipped with atomizer more than two, and annular
34 inner surface of water pipe is equipped with annular-heating piece, and electric telescopic rod 33 and fan 35 are equipped with two groups, two groups of electric telescopic rods
33 are symmetrically fixed on 34 upper end of annular conduit, and two groups of fans 35 are symmetrically mounted on 21 lower face of end cap.
Specific embodiment：In progress in use, staff first checks the utility model, check whether and deposit
In defect, it can not just carry out using if there is defect, need that maintenance personal is notified to carry out maintenance at this time, if do not deposited
It can be carried out using if problem, the utility model is placed in culture dish upper end by staff first, and then work people
Member injects stem cell by perfusion hole into perfusion bottle 1, and the personnel of proceeding with one's work pull up handle, and handle moves upwards drive and connects
Extension bar 22 moves upwards, and connecting rod 22 moves upwards band piston 24 and moves upwards, while return spring 23 is forced to compress, return
The compression of spring 23 generates screen resilience, and when piston 24, which is moved to, to be separated with outlet tube, stem cell flows to culture dish by outlet tube,
When needing to shut off immediately, staff unclamps hand, and return spring 23 will recover deformation under the action of screen resilience, and then drive
Connecting rod 22 moves downward, and final to realize that piston 24 returns to original position, realization is shut off immediately, which can stop perfusion at any time,
Perfusion flow preferably is controlled, prevents that perfusion flow is exceeded, improves the efficiency of stem cell culture.
Staff starts water pump, electric telescopic rod 33 and annular-heating piece simultaneously, and pump working will be in water tank 31
Water is pumped to hose 32, and water flows to annular conduit 34 by hose 32, and annular-heating piece works the water in annular conduit 34
Heating works at the same time personnel and opens atomizer, and hot water will uniformly be sprayed to 1 inner surface of perfusion bottle by atomizer, simultaneously
The work of electric telescopic rod 33 drives annular conduit 34 to move downward, and then 1 inner surface of perfusion bottle is cleaned completely, then
Staff opens fan 35, and the air flow in perfusion bottle 1 is accelerated in the work of fan 35, improves cleaning efficiency, which realizes
The function of cleaning perfusion bottle 1, improves the cleannes of perfusion bottle 1, and then improves perfusion quality.
The basic principle of the utility model and main feature and the advantages of the utility model has been shown and described above, for
For those skilled in the art, it is clear that the utility model is not limited to the details of above-mentioned exemplary embodiment, and without departing substantially from this
In the case of the spirit or essential attributes of utility model, the utility model can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the utility model is by institute
Attached claim rather than above description limit, it is intended that will fall within the meaning and scope of the equivalent requirements of the claims
All changes are embraced therein.Any reference numeral in claim should not be considered as to the involved right of limitation
It is required that.
Moreover, it will be appreciated that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
Using specification as an entirety, the technical solutions in each embodiment can also be properly combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (6)
1. a kind of stem cell culture perfusing device, including perfusion bottle (1), perfusion mechanism (2) and cleaning mechanism (3), feature
It is：Perfusion bottle (1) the upper end installation perfusion mechanism (2), the cleaning mechanism (3) are assemblied in perfusion mechanism (2) upper surface；
The perfusion mechanism (2) includes end cap (21), connecting rod (22), return spring (23) and piston (24), the end cap
(21) perfusion bottle (1) upper end is placed in, and end cap (21) is in connecting rod (22) annular side, the return spring (23)
End is fixed on end cap (21) lower end, and return spring (23) lower end is mounted on connecting rod (22) annular side medium position, described
Piston (24) is fixed on connecting rod (22) lower end, the perfusion bottle (1) be internally provided with connecting rod (22), return spring (23) and
Piston (24)；
The cleaning mechanism (3) includes water tank (31), hose (32), electric telescopic rod (33), annular conduit (34) and fan
(35), the water tank (31) is fixed on end cap (21) upper surface, and the hose (32) is assemblied in water tank (31) right end, described electronic
Telescopic rod (33) upper end is fixed on end cap (21) lower face, and electric telescopic rod (33) lower end is equipped with annular conduit (34),
Hose (32) lower end is connected through end cap (21) with annular conduit (34), and the fan (35) is assemblied under end cap (21)
End face, fan (35) outside are equipped with annular conduit (34), electric telescopic rod (33) are equipped on the outside of the annular conduit (34).
2. a kind of stem cell culture perfusing device according to claim 1, it is characterised in that：Perfusion bottle (1) lower end
Outlet tube is installed, the perfusion bottle (1) is connected by outlet tube with culture dish, and the piston (24) is arranged in outlet tube
Surface.
3. a kind of stem cell culture perfusing device according to claim 1, it is characterised in that：End cap (21) upper surface
Perfusion hole is offered, end cap (21) upper surface offers through hole, and sliding sleeve is equipped in through hole, and the connecting rod (22) is logical
Sliding sleeve and through hole is crossed with end cap (21) to be connected.
4. a kind of stem cell culture perfusing device according to claim 1, it is characterised in that：Connecting rod (22) upper end
Handle is installed, and handle is connected by fixing bolt with connecting rod (22), and handle outer end is equipped with anti-skid chequer.
5. a kind of stem cell culture perfusing device according to claim 1, it is characterised in that：Water tank (31) inner bottom
End is equipped with water pump, and hose (32) left end is connected through water tank (31) with water pump, annular conduit (34) lower face
Atomizer more than two is uniformly installed, and annular conduit (34) inner surface is equipped with annular-heating piece.
6. a kind of stem cell culture perfusing device according to claim 1, it is characterised in that：The electric telescopic rod (33)
And fan (35) is equipped with two groups, electric telescopic rod (33) described in two groups is symmetrically fixed on annular conduit (34) upper end, two groups
The fan (35) is symmetrically mounted on end cap (21) lower face.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721406242.6U CN207418758U (en) | 2017-10-26 | 2017-10-26 | A kind of stem cell culture perfusing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721406242.6U CN207418758U (en) | 2017-10-26 | 2017-10-26 | A kind of stem cell culture perfusing device |
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| Publication Number | Publication Date |
|---|---|
| CN207418758U true CN207418758U (en) | 2018-05-29 |
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ID=62309126
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721406242.6U Active CN207418758U (en) | 2017-10-26 | 2017-10-26 | A kind of stem cell culture perfusing device |
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| Country | Link |
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| CN (1) | CN207418758U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109251858A (en) * | 2018-09-20 | 2019-01-22 | 南京至泰生物医药科技有限公司 | Stem cell culture perfusing device |
| CN111826287A (en) * | 2020-08-04 | 2020-10-27 | 湖北明德健康科技有限公司 | Improved cell culture perfusion device |
-
2017
- 2017-10-26 CN CN201721406242.6U patent/CN207418758U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109251858A (en) * | 2018-09-20 | 2019-01-22 | 南京至泰生物医药科技有限公司 | Stem cell culture perfusing device |
| CN111826287A (en) * | 2020-08-04 | 2020-10-27 | 湖北明德健康科技有限公司 | Improved cell culture perfusion device |
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